| isolation and partial characterization of a marine bacteriophage. | | 1972 | 4663505 |
| action of staphylococcal epidermolytic toxin on mouse skin in organ culture. | | 1972 | 4675031 |
| isolation and characterization of tubules and plasma membranes from cytophaga columnaris. | tubular structures are released from cells of cytophaga columnaris after lysis of the cells. to determine the nature of these tubules, they were purified and their composition was determined. tubules were isolated after treating cell lysates with 1.0% sodium dodecyl sulfate at ph 8.1, which solubilizes all structural components except tubules. plasma membranes from the same organism were isolated by discontinuous sucrose gradient centrifugation of lysed cells. both tubules and membranes are comp ... | 1973 | 4712571 |
| characterization of the -d-glucan from the plastids of cecropia peltata as a glycogen-type polysaccharide. | | 1973 | 4738054 |
| studies on beta-glucanases. some properties of a bacterial endo-beta-(1 leads to 3)-glucanase system. | a commercial enzyme preparation, originally obtained from a flavobacterium(cytophaga), was fractionated by continuous electrophoresis, giving a protein fraction which hydrolysed laminarin, carboxymethylpachyman, barley beta-glucan, lichenin and cellodextrin in random fashion. this enzymic activity was not very stable. ion-exchange chromatography and molecular-sieve chromatography on bio-gel p-60 showed that this activity was due to two specific beta-glucanases, an endo-beta-(1-->3)-glucanase and ... | 1973 | 4776863 |
| [protonated polynucleotides. v. study of the protonation of nucleic acids and polynucleotides by optical rotatory dispersion and circular dichroism]. | | 1968 | 4890846 |
| cytophaga that kills or lyses algae. | a myxobacterium (cytophaga n-5) isolated from sewage kills or lyses an array of living green and blue-green algae. when assayed with nostoc muscorum or plectonema boryanum, plaques form like those caused by the blue-green algal virus lpp-1. this isolate lyses or inhibits mutually gram-positive and gram-negative eubacteria. | 1969 | 4891858 |
| production of a cholinesterase-solubilizing factor from a cytophaga sp. by continuous cultivation. | | 1969 | 4891885 |
| variations in the metal content of some commercial media and their effect on microbial growth. | the cation content in commercial media obtained from two manufacturers showed considerable variation. even different batches of the same make were found to be inconsistent in the content of metal ions. with cultures of cytophaga sp. and yersinia pseudotuberculosis in base media, growth stimulation was dependent on additions of certain commercial media. it could be demonstrated that this stimulation was derived solely from increased mg(2+) concentration in the media. | 1971 | 4940866 |
| an initial approach to the taxonomy of some gram negative yellow pigmented rods. | | 1971 | 4947438 |
| gamma-irradiation of deoxyribonucleic acid in dilute solutions. i. a sensitive method for detection of single-strand breaks in polydisperse dna samples. | | 1967 | 4962560 |
| increased extracellular production of a cholinesterase-solubilising factor by cytophaga ncmb 1314 during magnesium starvation. | a cytophaga sp. with the property of liberating a cholinesterase which is found in body muscle of plaice was studied. the liberation was caused by a factor of which more than 90% was found outside the bacterial cell and might possibly be associated within the slime material surrounding the bacteria. magnesium limitation during growth of cytophaga sp. in batch cultures resulted in an about 10-fold increase in extracellular factor activity. the increase could be immediately stopped by addition of ... | 1978 | 638892 |
| isolation and preliminary characterization of an extracellular protease of cytophaga sp. | | 1971 | 5000404 |
| the adansonian taxonomy and the deoxyribonucleic acid base composition of some gram negative, yellow pigmented rods. | | 1972 | 5025432 |
| sensitivity of cellulolytic bacteria to antibiotics. | the sensitivity of eight cellulolytic bacterial strains to eight antibiotics was tested. the results showed that, in general, the strains belonging to cytophaga, cellvibrio, and cellfalcicula are more sensitive to antibiotics than those strains that belong to sporocytophaga and cellulomonas. the inhibitory activity of the tested antibiotics, though differing with different strains, showed the following categories: tetracycline, erythromycin, and chloromycetin were most active, kanamycin, strepto ... | 1977 | 414477 |
| breakdown of chitin by cytophaga johnsonii. | | 1972 | 5073232 |
| myxobacteria exert partial control of damping-off and root disease in container-grown tree seedlings. | | 1972 | 5081120 |
| an electron microscope study of the cell surface of cytophaga johnsonii and some observations on related organisms. | | 1965 | 5296353 |
| a comparison of the lytic action of cytophaga johnsonii on a eubacterium and a yeast. | | 1967 | 5298433 |
| the history, biology, and taxonomy of the cytophaga group. | the first section of this review covers the important characteristics of the genera cytophaga and sporocytophaga. the topics discussed include vegetative cell structure, the spreading habit, and degradation of macromolecules. a historical account of these two genera follows, together with a discussion on the definition of, and species differentiation with the genus cytophaga, and on the taxonomy of sporocytophaga. the third section deals with the relationships of the cytophagas with the flavobac ... | 1977 | 413616 |
| [some new data on the ecology and properties of a bacterium belonging to the promyxobacterium genus]. | | 1969 | 5345724 |
| bacteriological study of laboratory-reared juvenile american oysters (crassostrea virginica). | | 1969 | 5371083 |
| characterization of cdc group df-3 by cellular fatty acid analysis. | fourteen strains of centers for disease control group df-3 bacteria were examined for cellular fatty acid composition to evaluate their chemical relatedness to known bacterial species and groups. the fatty acids were liberated from whole cells by base hydrolysis, methylated, and analyzed by capillary gas-liquid chromatography. all group df-3 strains possessed a distinct fatty acid profile which was characterized by large amounts (24%) of 12-methyltetradecanoate (a-c15:0), moderate amounts of sat ... | 1989 | 2542365 |
| iso-branched 2- and 3-hydroxy fatty acids as characteristic lipid constituents of some gliding bacteria. | the fatty acids present in the total hydrolysates of several gliding bacteria (myxococcus fulvus, stigmatella aurantiaca, cytophaga johnsonae, cytophaga sp. strain samoa and flexibacter elegans) were analyzed by combined gas-liquid chromatography and mass spectrometry. in addition to 13-methyl-tetradecanoic acid, 15-methyl-hexadecanoic acid, hexadecanoic acid, and hexadecenoic acid, 2- and 3-hydroxy fatty acids comprised up to 50% of the total fatty acids. the majority was odd-numbered and iso-b ... | 1979 | 118159 |
| solubilization of autoclaved feathers and wool by myxobacteria. | | 1969 | 5392470 |
| the cellulase system of a cytophaga species. | cellulases (ec 3.2.1.4) of a cytophaga species wthc 2421 (atcc 29474) were found in the soluble portion of the cell (the periplasm and the cytoplasm) and on the membrane. cell-free cellulases were not found. most of the carboxymethylcellulase activity associated with reduction of viscosity was membrane bound, whereas most of the carboxymethylcellulose (cmc) saccharifying activity was soluble. the cmc-saccharifying activity was increased 534 x by purification procedures which included ammonium su ... | 1977 | 20217 |
| the degradation of nucleic acids by cytophaga johnsonii. | | 1970 | 5448252 |
| [boron assimilation by cellulose-decomposing microorganisms]. | | 1970 | 5493353 |
| the separation of beta-glucanases produced by cytophaga johnsonii and their role in the lysis of yeast cell walls. | 1. when cytophaga johnsonii was grown in the presence of suitable inducers the culture fluid was capable of lysing thiol-treated yeast cell walls in vitro. 2. autoclaved or alkali-extracted cells, isolated cell walls and glucan preparations made from them were effective inducers, but living yeast cells or cells killed by minimal heat treatment were not. 3. chromatographic fractionation of lytic culture fluids showed the presence of two types of endo-beta-(1-->3)-glucanase and several beta-(1-->6 ... | 1970 | 5494230 |
| [polysaccharidases of sporocytophaga myxococcoides beta mannanase, cellulase and xylanase]. | | 1970 | 5507734 |
| endo-polygalacturonate lyase of cytophaga johnsonii. | | 1971 | 5559468 |
| purification of a cholinesterase from the body muscles of plaice (pleuronectes platessa). | | 1967 | 5585682 |
| [on the surface of myxobacteria. i. chemistry and morphology of the cell walls of cytophaga hutchinsonii and sporocytophaga myxococcoides]. | | 1967 | 5603104 |
| the extracellular agarase from a cytophaga species. | | 1968 | 5679384 |
| the effect of processing and marketing procedures on the bacteriological condition and shelf life of eviscerated turkeys. | | 1968 | 5691756 |
| [aerobic decomposition of cellulose and the active structure of soils (résumé of 20 years of research, 1946-1967)]. | | 1968 | 5697214 |
| [on the surface structure of myxobacteria. ii. anionic heteropolysaccharides as components of the slime layer of cytophaga hutchinsonii and sporocytophaga myxococcoides]. | | 1968 | 5709357 |
| gluconic acid forming bacteria from the soil: a new definition for the lwoffi-glucidolytica group of bacteria. | | 1968 | 5742778 |
| [the degradation of cellulose in the soil. enzymatic mechanisms. general report]. | | 1968 | 5748701 |
| cis-11-hexadecenoic acid from cytophaga hutchinsonii lipids. | | 1969 | 5773100 |
| problems concerning the relationships of cytophagas and flavobacteria. | | 1969 | 5791930 |
| the taxonomy, differentiation and identification of cytophaga species. | | 1969 | 5791934 |
| properties of some pectolytic, yellow pigmented, gram negative bacteria isolated from fresh cauliflowers. | | 1969 | 5791936 |
| the identification of gram-negative, nonfermentative bacteria from water: problems and alternative approaches to identification. | | 1986 | 2424280 |
| the pathogenic myxobacteria with special reference to fish diseases. | | 1969 | 5815479 |
| [studies on the cellulolytic activity of sporocytophaga myxococcoides]. | | 1965 | 5851824 |
| impaired defense mechanisms in bay mussels, mytilus edulis, with hemic neoplasia. | immunocompetence of bay mussels, mytilus edulis, with hemic neoplasia was investigated with an in vitro yeast phagocytosis assay and by in vivo clearance from the blood of injected cytophaga sp. bacteria. the yeast phagocytosis assay was conducted with hemocytes maintained in 90% plasma. neoplastic hemocytes, characterized by enlarged nuclei and scant cytoplasm, failed to phagocytose yeast cells. in contrast, greater than 90% of hemocytes from unaffected animals and morphologically normal hemocy ... | 1989 | 2723448 |
| [on the ecology of water myxobacteria (sporocytophaga cauliformis) in lake constance]. | | 1965 | 5858956 |
| production of gibberellin-like substances by bacteria and actinomycetes. | | 1965 | 5861292 |
| fine structure and host-virus relationship of a marine bacterium and its bacteriophage. | valentine, artrice f. (georgetown university, washington, d.c.), and george b. chapman. fine structure and host-virus relationship of a marine bacterium and its bacteriophage. j. bacteriol. 92:1535-1554. 1966.-the fine structure of a gram-negative marine bacterium, cytophaga marinoflava sp. n., has been revealed by ultrathin sectioning and electron microscopy. stages in the morphogenesis of the bacterial virus ncmb 385, which has been shown to be highly specific for this organism, were also demo ... | 1966 | 5924277 |
| properties of two marine bacteriophages. | chen, peter k. (georgetown university, washington, d.c.), ronald v. citarella, omar salazar, and rita r. colwell. properties of two marine bacteriophages. j. bacteriol. 91:1136-1139. 1966.-various properties have been determined for two bacteriophages, ncmb 384 and 385, and their host, ncmb 397, a cytophaga sp., isolated from the marine environment. the purified bacteriophages have been subjected to serological analysis, results of which indicate a high degree of relatedness. purified, highly po ... | 1966 | 5929746 |
| the solubilization of a cholinesterase from plaice muscle by bacteria. | | 1966 | 5941307 |
| a bacteriophage for myxococcus xanthus: isolation, characterization and relation of infectivity to host morphogenesis. | burchard, robert p. (university of minnesota, minneapolis), and m. dworkin. a bacteriophage for myxococcus xanthus: isolation, characterization and relation of infectivity to host morphogenesis. j. bacteriol. 91:1305-1313. 1966.-a bacteriophage (mx-1) infecting myxococcus xanthus fb(t) has been isolated from cow dung. the bacteriophage particle is approximately 175 mmu long. a tail about 100 mmu in length is encased in a contractile sheath and terminates in a tail plate. the head is polyhedral w ... | 1966 | 5948779 |
| dna base composition of cytophaga marinoflava n. sp. determined by buoyant density measurements in cesium chloride. | | 1966 | 5963325 |
| [study on the possibility of identification of b. anitratum and m. lwoffi (acinetobacter) by a serological method]. | | 1966 | 5976381 |
| chemistry and ultrastructure of surface layers in primitive myxobacteria: cytophaga hutchinsonii and sporocytophaga myxococcoides. | | 1967 | 6048423 |
| evidence for colonization and destruction of hinge ligaments in cultured juvenile pacific oysters (crassostrea gigas) by cytophaga-like bacteria. | several strains of cytophaga-like gliding bacteria (clb) were isolated as numerically dominant or codominant components of bacterial populations associated with proteinaceous hinge ligaments of cultured juvenile pacific oysters, crassostrea gigas. these bacteria were morphologically similar to long, flexible bacilli occurring within degenerative lesions in oyster hinge ligaments. among bacteria isolated from hinge ligaments, only clb strains were capable of sustained growth with hinge ligament m ... | 1989 | 2757377 |
| the enzymic degradation of porphyran. | 1. the algal galactan, porphyran, was incubated with enzymes from a cytophaga sp. and the products were examined. 2. only about 30% of the porphyran was recovered in the form of oligosaccharides, the remainder being of high molecular weight. 3. among the saccharides were d-galactose, 6-o-methyl-d-galactose, neoagarobiose, neoagarotetraose and oligosaccharides containing 6-o-methyl-d-galactose, the principal of which has been tentatively identified as 6(3)-o-methyl-neoagarotetraose. fragments con ... | 1967 | 6060448 |
| numerical taxonomy of some named bacterial cultures. | | 1967 | 6064342 |
| effect of packaging on the major microbial flora of irradiated haddock. | | 1967 | 6067600 |
| phenotypic and genomic studies of "cytophaga psychrophila" isolated from diseased rainbow trout (oncorhynchus mykiss) in france. | five strains of gliding bacteria were isolated in france from farmed diseased rainbow trouts reared at low water temperature. the resemblance of these bacteria to the known fish pathogen "cytophaga psychrophila" led to their comparative study with reference strain ncmb 1947 and with an american isolate. morphological, physiological, and biochemical characteristics of the seven strains proved to be similar. comparison of their dna by the s1 nuclease dna-dna hybridization method showed that the se ... | 1989 | 2764577 |
| coaggregation of human oral cytophaga species and actinomyces israelii. | a total of 19 strains of oral cytophaga sp. obtained from subgingival plaque deposits were tested for their ability to coaggregate with strains of actinomyces israelii, a. viscosus, a. naeslundii, streptococcus sanguis, s. mutans, s. salivarius, and s. mitis. coaggregation was observed only with a. israelii. based on their coaggregation patterns with eight a. israelii strains, the cytophaga strains were distributed among three distinct groups: those that coaggregated with a. israelii pk16 but no ... | 1983 | 6133836 |
| studies on psychrophilic bacteria in two lakes of different trophy. | the number of bacteria capable of growth at low temperatures in two lakes was found to be subject to considerable variation throughout the year although the changes were not correlated with changes in ambient temperature. a correlation was, however, observed between temperature and ratio of number of psychrophiles to "total" count. all of the psychrophilic bacteria isolated from the studied lakes were able to grow at temperatures below 0 degrees c. microorganisms isolated from eutrophic lakes we ... | 1981 | 6174030 |
| studies on pectolytic bacteria in water and bottom sediments of two lakes of different trophy. | pectolytic bacteria were found in greater numbers in the eutrophic than in the mesotrophic lake. however, higher pectolytic activity was exhibited by the bacteria derived from the mesotrophic lake. in the eutrophic lake higher endo- and exo-pg activity was found in the benthic than in the panktonic bacteria, whereas in the mesotrophic one higher endo-pg activity was exhibited by the planktonic and higher exo-pg activity by the benthic bacteria. the polygalacturonases synthesized by the bacteria ... | 1982 | 6189379 |
| interference reflection microscopic study of sites of association between gliding bacteria and glass substrata. | sites of close contact between gliding cytophaga sp. strain u67 cells and glass were examined by interference reflection microscopy. site patterns changed during translocation and moved relative to the substratum, in contrast to previous interference reflection microscopy observations of fibroblast and amoeboid motility. sinistral rotation around the long axis of the cell was coupled with gliding, except when curved cells traversed curvilinear pathways. close contact was temporary, since cells f ... | 1989 | 2768185 |
| unusual sulfonolipids are characteristic of the cytophaga-flexibacter group. | capnocytophaga spp. contain a group of unusual sulfonolipids, called capnoids (w. godchaux iii and e. r. leadbetter, j. bacteriol. 144:592-602, 1980). one of these lipids, capnine, is 2-amino-3-hydroxy-15-methylhexadecane-1-sulfonic acid; the others are, apparently, n-acylated versions of capnine. the lipids were found, in amounts ranging from 2.5 to 16 mumol of capnoid sulfur per g of cells (wet weight), in two cytophaga spp. and also in several closely related organisms: several capnocytophaga ... | 1983 | 6298180 |
| biosynthesis of a sulfonolipid in gliding bacteria. | gliding bacteria of the genus cytophaga synthesize sulfonolipids (1,2) that contain capnine (1-deoxy-15-methylhexadecasphinganine-1-sulfonic acid). studies of the incorporation of radiolabeled compounds by c. johnsonae show that cysteate is utilized preferentially to both cystine and inorganic sulfate as a precursor of capnine sulfur and to both cystine and serine as a precursor of carbons 1 and 2 of capnine. the results are consistent with a pathway in which capnine is formed by condensation of ... | 1985 | 2992489 |
| biosynthesis of the sulfonolipid 2-amino-3-hydroxy-15-methylhexadecane-1-sulfonic acid in the gliding bacterium cytophaga johnsonae. | the biosynthesis of the sulfonolipid 2-amino-3-hydroxy-15-methylhexadecane-1-sulfonic acid (capnine) was studied by measuring the incorporation of possible precursors into the lipid by cells grown in the presence of precursors which were labeled with stable isotopes. cells grown on yeast extract in the presence of dl-[3,3-2h2]serine contained 40.1 mol% of the protein-bound serine and 5.0 mol% of the protein-bound cysteine derived from the labeled serine. cells grown in the presence of dl-[3,3-2h ... | 1984 | 6330048 |
| a cytophaga species endotoxin as a putative agent of occupation-related lung disease. | a previous study suggested that a biologically active bacterial endotoxin was a putative agent of lung disease in a textile-producing facility. the endotoxin was isolated from the biomass growing in a chilled-water spray air humidification system. the bacterial flora of the air humidification system were isolated and taxonomically identified to the genus level. by using indirect immunofluorescence assays, a serologically reactive cytophaga species was identified. a serologically reactive, biolog ... | 1984 | 6360896 |
| temperate phages and bacteriocins of the gliding bacterium cytophaga johnsonae. | a collection of 30 independently isolated strains of cytophaga johnsonae was screened for the presence of temperate bacteriophages. two strains were found to harbour phages. the newly isolated phages differ in several respects from the 43 previously isolated phages for c. johnsonae. both phages are polyhedral, approximately 60 nm in diameter, and have no apparent tail structure. they are chloroform sensitive, and plaque formation is inhibited by agar. both are capable of establishing a stable as ... | 1988 | 3171539 |
| isolation and characterization of katanosins a and b. | two peptide antibiotics katanosins a and b were isolated from the culture broth of a strain related to the genus cytophaga. these antibiotics are basic peptides soluble in aqueous alcohols. the molecular formulae c57h95n15o17 for a and c58h97n15o17 for b were indicated. the constituent amino acids of katanosin a are suggested to be thr (1), ser (1), val (1), leu (3), arg (1) and three unusual amino acids. in katanosin b, the val residue is replaced by ile. katanosins a and b are active against g ... | 1988 | 3403364 |
| identification and evolution of the cellulolytic microflora present during composting of cattle manure: on the role of actinomycetes sp. | population changes in cellulolytic microflora were studied during composting of cattle manure. six bacterial and nine actinomycetes species were isolated. among the isolates, two actinomycetes, micromonospora chalcae and pseudonocardia thermophila, were cellulolytic strains which were numerically dominant and might therefore play a notable role in the degradation of cellulose in cattle manure. fungi were practically absent during the process, but two cellulose-degrading bacterial species, sporoc ... | 1984 | 6508072 |
| isolation and characterization of a new cytophaga species implicated in a work-related lung disease. | a yellow-pigmented, gram-negative, gliding bacterium isolated from an industrial water spray air humidification system was implicated as a causative agent in several occurrences of lung disease with hypersensitivity pneumonitis-like symptoms. the bacterium, designated wf-164, lacked microcysts or fruiting bodies and had a dna base composition of 34.8 mol% of guanine plus cytosine. gliding, flexing, nonflagellated cells measuring 0.3 by 3.5 to 8.9 micron were observed by using light and electron ... | 1984 | 6508308 |
| evidence for the participation of n-acetylated amino sugars in the coaggregation between cytophaga species strain dr2001 and actinomyces israelii pk16. | coaggregation between cytophaga sp. strain dr2001 and actinomyces israelii pk16 was partially inhibited by certain n-acetylated amino sugars (n-acetylneuraminic acid, n-acetylgalactosamine, and n-acetylglucosamine) and was completely inhibited by the trisaccharide neuraminin-lactose. the monosaccharides exerted their effect at concentrations between 30 to 100 mm, whereas the trisaccharide was an effective inhibitor at significantly lower concentrations. outer membrane preparations caused a. isra ... | 1984 | 6715035 |
| induction of human immunoglobulin synthesis (igg, iga) by the novel, t cell independent mitogen cytophaga allerginae endotoxin. | cytophaga allerginae endotoxin (cae) has been purified from c. allerginae, a newly discovered bacterial species isolated from a chilled water spray humidification system. the present study was undertaken in order to determine whether cae can induce immunoglobulin synthesis by human peripheral blood lymphocytes (pbl) in culture. to this end, human pbl were purified and cultured with either pokeweed mitogen at 5 micrograms/ml, or cae (at varying concentrations) for 6 days. the levels of igg and ig ... | 1987 | 3497882 |
| trail following by gliding bacteria. | slime trails, which are deposited on surfaces by gliding bacteria and which serve as preferential pathways for gliding motility, were tested for the species specificity of their support of movement. among the pairs of bacteria tested, a variety of gliding bacteria and a flagellated bacterium moved along trails of unrelated species. thus, the trails did not serve as pheromones. rather, they may have guided gliding elasticotactically. some biological implications of this finding are considered. | 1982 | 6811562 |
| pb-5266 a, b and c, new monobactams. i. taxonomy, fermentation and isolation. | new monobactams, pb-5266 a, b and c were isolated from the culture filtrate of cytophaga johnsonae pb-5266 by various types of column chromatography and preparative reverse phase hplc. pb-5266 a, b and c exhibited weak antibacterial activity against a sensitive mutant of escherichia coli to beta-lactam antibiotics. | 1987 | 3570960 |
| pb-5266 a, b and c, new monobactams. ii. physico-chemical properties and chemical structures. | the chemical structures of three new monobactams, pb-5266 a, b and c, were elucidated by their physico-chemical properties and spectrometric studies. in contrast to previously described monobactams, they all possess a dehydroasparagine residue. | 1987 | 3570961 |
| capnocytophaga septicemia. | | 1980 | 7360185 |
| deoxyribonucleic acid relatedness of some menaquinone-producing flavobacterium and cytophaga strains. | nine menaquinone-forming strains of the flavobacterium--cytophaga complex with dna base compositions between 35 and 45 moles percent guanine-plus-cytosine were investigated for genome sizes and dna relatedness by dna:dna hybridization in vitro, using the optically recorded initial reassociation kinetics. two strains representing c. hutchinsonii and c. marinoflava proved to be related on the 50 percent binding level, i.e. on a level of dna relatedness commonly found within well-classified convent ... | 1980 | 7396480 |
| cell wall and lipid composition of isosphaera pallida, a budding eubacterium from hot springs. | isosphaera pallida is an unusual gliding, budding eubacterium recently isolated from north american hot springs. electron micrographs of ultrathin sections revealed a cell wall atypical of eubacteria: two electrondense layers separated by an electron-transparent layer, with no evident peptidoglycan layer. growth was not inhibited by penicillin. cell walls were isolated from sheared cells by velocity sedimentation. the rigid-layer fraction, prepared from cell walls by treatment with boiling 10% s ... | 1987 | 3584067 |
| purification and characterization of a raw-starch digesting amylase from a soil bacterium--cytophaga sp. | a newly isolated bacterium from soil, identified as cytophaga sp. was found to produce raw-starch digesting amylase. the enzyme was purified from 24-hr cultured medium through ammonium sulfate fractionation, deae-sepharose cl 6b ion exchange chromatography and sephacryl s-200 gel filtration. the preparation was proved to be homogeneous by sds-page. the subunit molecular weight determined by sds-page was 59 kd. the optimum temperature was 50 degrees c on soluble starch and 60 degrees c on raw sta ... | 1995 | 7539663 |
| purification of a kappa-carrageenase from marine cytophaga species. | a mixture of extracellular carrageenases was isolated from the cell-free medium of a culture of marine cytophaga sp. 1k-c783 grown on zobell 2216 e broth with 0.1% commercial carrageenan. a single active peak of kappa-carrageenase was separated and purified from the mixture by ammonium sulfate precipitation, ion-exchange chromatography, and sephadex g-200 gel filtration chromatography. molecular weight of the purified kappa-carrageenase was estimated as 100,000 by sodium dodecyl sulfate-polyacry ... | 1987 | 3696007 |
| a numerical taxonomic study of flavobacterium-cytophaga strains from dairy sources. | phenotypic data on 203 gram-negative non-fermentative bacteria of the flavobacterium-cytophaga group isolated from milk and butter were analyzed by numerical taxonomic techniques. twenty reference strains including species of flavobacterium, cytophaga and strains of pseudomonas paucimobilis were included in the study. using the matching coefficient of sokal & michener with antibiotic susceptibility data included, 189 isolates were recovered in nine clusters. six of these clusters were linked at ... | 1985 | 4066550 |
| biodiversity of gas vacuolate bacteria from antarctic sea ice and water. | psychrophilic, gas vacuolate, heterotrophic bacteria indigenous to sea ice communities in antarctica have been isolated. phylogenetic analysis of representative members of these bacteria shows that they belong to the alpha, beta, and gamma proteobacteria and the flavobacteria-cytophaga group. this is the first report of gas vacuolate bacteria from the beta proteobacteria and the flavobacteria-cytophaga groups. | 1995 | 7574660 |
| fatty acids of myxococcus xanthus. | fatty acids were extracted from saponified vegetative cells and myxospores of myxococcus xanthus and examined as the methyl esters by gas-liquid chromatography. the acids consisted mainly of c(14) to c(17) species. branched acids predominated, and iso-pentadecanoic acid constituted half or more of the mixture. the other leading component (11-28%) was found to be 11-n-hexadecenoic acid. among the unsaturated acids were two diunsaturated ones, an n-hexadecadienoic acid and an iso-heptadecadienoic ... | 1973 | 4197903 |
| [the level of dna homology in species of the genus cytophaga]. | nucleotide composition, genome size and dna homologies were studied for strains cytophaga johnsonae dsm 425, c. pectinovora dsm 6368, c. saccharophyla dsm 1811, c. aquatilis dsm 2063, c. flevensis dsm 1076, c. lytica atcc 23 178, c. arvensicola dsm 3695, c. heparina dsm 2356. a considerable difference in gs-bases level between species of genus cytophaga (33.3-34.9 mol %) and incertae sedis species (c. heparina and c. arvensicola) (45.0-46.7 mol. %) was shown. the differentiation between freshwat ... | 1995 | 7655657 |
| phylogenetic diversity of the genus cytophaga revealed by 16s rrna sequencing and menaquinone analysis. | to clarify the intra- and intergeneric relationships of the genus cytophaga, 16s rrna sequences and respiratory isoprenoid quinones were determined for the type strains of the 21 validly published species and one isolate in the genus cytophaga. the sequence analysis revealed extreme heterogeneity of this genus, which diverged into nine distinct lines of descent. each lineage of cytophaga was characterized by possessing either menaquinone-6 (mk-6) or mk-7. the mk-6-possessing species were located ... | 1993 | 7689634 |
| selective release of recombinant protein particles (vlps) from yeast using a pure lytic glucanase enzyme. | we have used a pure lytic glucanase enzyme to selectively release recombinant 60 nm protein particles (virus like particles or vlps) from yeast cells. although the protease components of the lytic enzyme complexes were found to degrade the vlps, purified glucanase enzymes from these complexes (derived from cytophaga sp. and oerskovia sp.) produced cell lysis without degradation and released the vlps in the absence of mercaptoethanol, a reducing agent commonly used in cell lysis. the oerskovia gl ... | 1993 | 7764040 |
| polymorphism of natural dna. | | 1972 | 4349669 |
| direct evidence of the c-like form of sodium deoxyribonucleate. | from infrared linear dichroism studies, direct evidence is given for a new na dna form, which is similar to the c form of li dna. this c-like form in na dna occurs under conditions of very low nacl content (at high relative humidity), high nacl content (at low relative humidity), and low g + c content of the dna (at low relative humitiy). the c form of li dna was originally discovered by x-ray diffraction, but has not previously been characterized by infrared spectroscopy. the c form is primaril ... | 1973 | 4357868 |
| effect of amendments on the microbial utilization of oil applied to soil. | replicate field plots comprising a control, plus oil, plus oil and bacteria, plus oil and fertilizer (urea-phosphate; 27:27:0), and plus oil, bacteria, and fertilizer were monitored over a 308-day period for changes in bacterial and mold numbers. changes in the chemical composition of the oil applied to the plots was followed by using chromatographic techniques. application of fertilizer resulted in a stimulation of bacterial numbers and in the rate of utilization of n-alkane components of the s ... | 1974 | 4589125 |
| deoxyribonucleic acid homology in bacterial taxonomy: effect of incubation temperature on reaction specificity. | parameters affecting deoxyribonucleic acid duplex (dna-dna) formation on membrane filters were evaluated. the reference strains used were cytophaga succinicans strain 8, which has a guanine plus cytosine (gc) content of 38%, and myxococcus xanthus strain fb, which has a gc content of 70%. both organisms are gliding bacteria classified among the myxobacteria. among the parameters evaluated, the incubation temperature used during duplex formation was found to be the most important in terms of the ... | 1968 | 4966832 |
| facile purification of a -glucan hydrolase by chromatography on deae-cellulose. | | 1972 | 5057122 |
| [myxobacterial type quotient as an eutrophy indicator in surface waters]. | | 1965 | 5323597 |
| the action of a bacterial agarase on agarose, porphyran and alkali-treated porphyran. | 1. a purified extracellular agarase from a cytophaga species was used to hydrolyse agarose, porphyran and alkali-treated porphyran. 2. the hydrolysate from agarose was separated by gel filtration into the series of neoagarosaccharides, the predominant member of which was the tetrasaccharide. 3. enzyme action on alkali-treated porphyran gave neoagarosaccharides and other oligosaccharides containing 6-o-methyl-d-galactose units. from the composition of these oligosaccharides it is deduced that act ... | 1969 | 5386190 |
| tan-1057 a-d, new antibiotics with potent antibacterial activity against methicillin-resistant staphylococcus aureus. taxonomy, fermentation and biological activity. | two gram-negative bacteria were found to produce the new antibacterial antibiotics tan-1057 a, b, c and d. the producing bacteria were characterized and designated as flexibacter sp. pk-74 and pk-176. these antibiotics were active against gram-negative and gram-positive bacteria, including methicillin-resistant staphylococcus aureus. tan-1057 a inhibited protein biosynthesis in escherichia coli and s. aureus. it showed excellent protective effects against an experimental methicillin-resistant s. ... | 1993 | 8501003 |
| the specificity of an agarase from a cytophaga species. | 1. the extracellular agarase from a cytophaga species was shown to have no action on neoagarobiose, neoagarotetraose or their analogues containing 6-o-methyl-d-galactose residues. 2. the action of the enzyme on neoagaro-octaose suggests that scission of the central beta-d-galactosidic linkage, to form two molecules of tetrasaccharide, is the preferred mode of action; however, both exterior d-galactosidic linkages in the octasaccharide and both in neoagarohexaose are hydrolysed at a somewhat lowe ... | 1969 | 5386191 |
| fractionation of the beta-glucanases in a cytophage johnsonii culture filtrate lysing yeast cell walls. | | 1970 | 5420065 |
| the hydrolysis of algal galactans by enzymes from a cytophaga species. | 1. two bacteria were isolated from sea water by the enrichment culture technique, both of which could utilize the galactan sulphate, porphyran, as sole source of carbon. 2. from the cells of one bacterium, classified as a cytophaga sp., hydrolytic enzymes were isolated. 3. partial purification of the enzymes is described and some of the properties of the principal enzymes have been studied. 4. the action of the enzymes on several galactan sulphates of red algae suggests that an agarase is presen ... | 1967 | 6056629 |