Publications

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isolation and partial characterization of a marine bacteriophage. 19724663505
action of staphylococcal epidermolytic toxin on mouse skin in organ culture. 19724675031
isolation and characterization of tubules and plasma membranes from cytophaga columnaris.tubular structures are released from cells of cytophaga columnaris after lysis of the cells. to determine the nature of these tubules, they were purified and their composition was determined. tubules were isolated after treating cell lysates with 1.0% sodium dodecyl sulfate at ph 8.1, which solubilizes all structural components except tubules. plasma membranes from the same organism were isolated by discontinuous sucrose gradient centrifugation of lysed cells. both tubules and membranes are comp ...19734712571
comparison of the phosphatases of lysobacter enzymogenes with those of related bacteria.lysobacter enzymogenes atcc 29487 (uasm 495) produces an outer-membrane-associated phosphatase and an excreted phosphatase. the cell-associated enzyme was compared to phosphatases of nine other gram-negative gliding bacteria and to that of escherichia coli. the other three species of the genus lysobacter also produce a particulate, cell-associated phosphatase. antiserum prepared against the phosphatase from the outer membrane of l. enzymogenes effectively precipitated the phosphatases of two oth ...19872833562
studies on beta-glucanases. some properties of a bacterial endo-beta-(1 leads to 3)-glucanase system.a commercial enzyme preparation, originally obtained from a flavobacterium(cytophaga), was fractionated by continuous electrophoresis, giving a protein fraction which hydrolysed laminarin, carboxymethylpachyman, barley beta-glucan, lichenin and cellodextrin in random fashion. this enzymic activity was not very stable. ion-exchange chromatography and molecular-sieve chromatography on bio-gel p-60 showed that this activity was due to two specific beta-glucanases, an endo-beta-(1-->3)-glucanase and ...19734776863
[protonated polynucleotides. v. study of the protonation of nucleic acids and polynucleotides by optical rotatory dispersion and circular dichroism]. 19684890846
cytophaga that kills or lyses algae.a myxobacterium (cytophaga n-5) isolated from sewage kills or lyses an array of living green and blue-green algae. when assayed with nostoc muscorum or plectonema boryanum, plaques form like those caused by the blue-green algal virus lpp-1. this isolate lyses or inhibits mutually gram-positive and gram-negative eubacteria.19694891858
production of a cholinesterase-solubilizing factor from a cytophaga sp. by continuous cultivation. 19694891885
variations in the metal content of some commercial media and their effect on microbial growth.the cation content in commercial media obtained from two manufacturers showed considerable variation. even different batches of the same make were found to be inconsistent in the content of metal ions. with cultures of cytophaga sp. and yersinia pseudotuberculosis in base media, growth stimulation was dependent on additions of certain commercial media. it could be demonstrated that this stimulation was derived solely from increased mg(2+) concentration in the media.19714940866
an initial approach to the taxonomy of some gram negative yellow pigmented rods. 19714947438
gamma-irradiation of deoxyribonucleic acid in dilute solutions. i. a sensitive method for detection of single-strand breaks in polydisperse dna samples. 19674962560
changes in the cell surface of the dimorphic forms of candida albicans by treatment with hydrolytic enzymes.the release of acid phosphatase and polysaccharide-peptide complexes by hydrolytic enzymes from the surface of the blastospore and mycelial forms of candida albicans has been examined in cells from 4 h and 18 h cultures and the results correlated with the appearance of the treated cells in the electron microscope. treatment with dithiothreitol was necessary for the degradative action of the enzymes to occur. material released by all the treatments used had a similar qualitative composition, but ...1976784907
isolation and preliminary characterization of an extracellular protease of cytophaga sp. 19715000404
the adansonian taxonomy and the deoxyribonucleic acid base composition of some gram negative, yellow pigmented rods. 19725025432
facile purification of a -glucan hydrolase by chromatography on deae-cellulose. 19725057122
biosynthesis of a sulfonolipid in gliding bacteria.gliding bacteria of the genus cytophaga synthesize sulfonolipids (1,2) that contain capnine (1-deoxy-15-methylhexadecasphinganine-1-sulfonic acid). studies of the incorporation of radiolabeled compounds by c. johnsonae show that cysteate is utilized preferentially to both cystine and inorganic sulfate as a precursor of capnine sulfur and to both cystine and serine as a precursor of carbons 1 and 2 of capnine. the results are consistent with a pathway in which capnine is formed by condensation of ...19852992489
myxobacteria exert partial control of damping-off and root disease in container-grown tree seedlings. 19725081120
an electron microscope study of the cell surface of cytophaga johnsonii and some observations on related organisms. 19655296353
a comparison of the lytic action of cytophaga johnsonii on a eubacterium and a yeast. 19675298433
a note on the microbiology of retail packs of prepared salad vegetables.retail packs of mixed, prepared salad vegetables from two different manufacturers were stored at 7 degrees c until the end of storage-life (sell-by date plus 1 d), when the microbial flora was examined. the quality of the salads was acceptable at the end of storage life. the oxygen concentrations in packs were lower, and the carbon dioxide concentrations were higher, than those in air. high numbers of bacteria were present, with pseudomonas spp. and enterobacter agglomerans predominating in pack ...19873126172
[some new data on the ecology and properties of a bacterium belonging to the promyxobacterium genus]. 19695345724
bacteriological study of laboratory-reared juvenile american oysters (crassostrea virginica). 19695371083
the action of a bacterial agarase on agarose, porphyran and alkali-treated porphyran.1. a purified extracellular agarase from a cytophaga species was used to hydrolyse agarose, porphyran and alkali-treated porphyran. 2. the hydrolysate from agarose was separated by gel filtration into the series of neoagarosaccharides, the predominant member of which was the tetrasaccharide. 3. enzyme action on alkali-treated porphyran gave neoagarosaccharides and other oligosaccharides containing 6-o-methyl-d-galactose units. from the composition of these oligosaccharides it is deduced that act ...19695386190
temperate phages and bacteriocins of the gliding bacterium cytophaga johnsonae.a collection of 30 independently isolated strains of cytophaga johnsonae was screened for the presence of temperate bacteriophages. two strains were found to harbour phages. the newly isolated phages differ in several respects from the 43 previously isolated phages for c. johnsonae. both phages are polyhedral, approximately 60 nm in diameter, and have no apparent tail structure. they are chloroform sensitive, and plaque formation is inhibited by agar. both are capable of establishing a stable as ...19883171539
solubilization of autoclaved feathers and wool by myxobacteria. 19695392470
fractionation of the beta-glucanases in a cytophage johnsonii culture filtrate lysing yeast cell walls. 19705420065
the degradation of nucleic acids by cytophaga johnsonii. 19705448252
[boron assimilation by cellulose-decomposing microorganisms]. 19705493353
the separation of beta-glucanases produced by cytophaga johnsonii and their role in the lysis of yeast cell walls.1. when cytophaga johnsonii was grown in the presence of suitable inducers the culture fluid was capable of lysing thiol-treated yeast cell walls in vitro. 2. autoclaved or alkali-extracted cells, isolated cell walls and glucan preparations made from them were effective inducers, but living yeast cells or cells killed by minimal heat treatment were not. 3. chromatographic fractionation of lytic culture fluids showed the presence of two types of endo-beta-(1-->3)-glucanase and several beta-(1-->6 ...19705494230
[polysaccharidases of sporocytophaga myxococcoides beta mannanase, cellulase and xylanase]. 19705507734
endo-polygalacturonate lyase of cytophaga johnsonii. 19715559468
purification of a cholinesterase from the body muscles of plaice (pleuronectes platessa). 19675585682
[on the surface of myxobacteria. i. chemistry and morphology of the cell walls of cytophaga hutchinsonii and sporocytophaga myxococcoides]. 19675603104
the extracellular agarase from a cytophaga species. 19685679384
the effect of processing and marketing procedures on the bacteriological condition and shelf life of eviscerated turkeys. 19685691756
[aerobic decomposition of cellulose and the active structure of soils (résumé of 20 years of research, 1946-1967)]. 19685697214
[on the surface structure of myxobacteria. ii. anionic heteropolysaccharides as components of the slime layer of cytophaga hutchinsonii and sporocytophaga myxococcoides]. 19685709357
gluconic acid forming bacteria from the soil: a new definition for the lwoffi-glucidolytica group of bacteria. 19685742778
[the degradation of cellulose in the soil. enzymatic mechanisms. general report]. 19685748701
a distributed model of enzymatic lysis of microbial cells. 19873324869
problems concerning the relationships of cytophagas and flavobacteria. 19695791930
the taxonomy, differentiation and identification of cytophaga species. 19695791934
properties of some pectolytic, yellow pigmented, gram negative bacteria isolated from fresh cauliflowers. 19695791936
purification and characterization of chloramphenicol acetyltransferase from flavobacterium cb60.from the highly chloramphenicol-resistant cytophaga-like bacterium flavobacterium cb60, which can both acetylate chloramphenicol and degrade it in co-metabolism, the chloramphenicol acetyltransferase (cat) was purified to homogeneity and characterized. the purification included fractional precipitation with ammonium sulphate and two affinity chromatography steps, eluting cat the first time with 5 mm-chloramphenicol and the second time with a linear gradient (0-10 mm) of chloramphenicol. the puri ...19873327915
the pathogenic myxobacteria with special reference to fish diseases. 19695815479
pb-5266 a, b and c, new monobactams. i. taxonomy, fermentation and isolation.new monobactams, pb-5266 a, b and c were isolated from the culture filtrate of cytophaga johnsonae pb-5266 by various types of column chromatography and preparative reverse phase hplc. pb-5266 a, b and c exhibited weak antibacterial activity against a sensitive mutant of escherichia coli to beta-lactam antibiotics.19873570960
isolation and characterization of filterable marine bacteria.anderson, j. i. w. (northeast shellfish sanitation research center, narragansett, r.i.), and w. p. heffernan. isolation and characterization of filterable marine bacteria. j. bacteriol 90:1713-1718. 1965.-by a process of double filtration of seawater, first through a membrane filter with a pore diameter of 0.45 mu and then through a membrane filter with a pore diameter of 0.22 mu, it was possible to isolate on the surface of the latter membrane a group of marine organisms not usually encountered ...19655854592
[on the ecology of water myxobacteria (sporocytophaga cauliformis) in lake constance]. 19655858956
production of gibberellin-like substances by bacteria and actinomycetes. 19655861292
pb-5266 a, b and c, new monobactams. ii. physico-chemical properties and chemical structures.the chemical structures of three new monobactams, pb-5266 a, b and c, were elucidated by their physico-chemical properties and spectrometric studies. in contrast to previously described monobactams, they all possess a dehydroasparagine residue.19873570961
cell wall and lipid composition of isosphaera pallida, a budding eubacterium from hot springs.isosphaera pallida is an unusual gliding, budding eubacterium recently isolated from north american hot springs. electron micrographs of ultrathin sections revealed a cell wall atypical of eubacteria: two electrondense layers separated by an electron-transparent layer, with no evident peptidoglycan layer. growth was not inhibited by penicillin. cell walls were isolated from sheared cells by velocity sedimentation. the rigid-layer fraction, prepared from cell walls by treatment with boiling 10% s ...19873584067
the solubilization of a cholinesterase from plaice muscle by bacteria. 19665941307
a bacteriophage for myxococcus xanthus: isolation, characterization and relation of infectivity to host morphogenesis.burchard, robert p. (university of minnesota, minneapolis), and m. dworkin. a bacteriophage for myxococcus xanthus: isolation, characterization and relation of infectivity to host morphogenesis. j. bacteriol. 91:1305-1313. 1966.-a bacteriophage (mx-1) infecting myxococcus xanthus fb(t) has been isolated from cow dung. the bacteriophage particle is approximately 175 mmu long. a tail about 100 mmu in length is encased in a contractile sheath and terminates in a tail plate. the head is polyhedral w ...19665948779
dna base composition of cytophaga marinoflava n. sp. determined by buoyant density measurements in cesium chloride. 19665963325
[study on the possibility of identification of b. anitratum and m. lwoffi (acinetobacter) by a serological method]. 19665976381
chemistry and ultrastructure of surface layers in primitive myxobacteria: cytophaga hutchinsonii and sporocytophaga myxococcoides. 19676048423
the hydrolysis of algal galactans by enzymes from a cytophaga species.1. two bacteria were isolated from sea water by the enrichment culture technique, both of which could utilize the galactan sulphate, porphyran, as sole source of carbon. 2. from the cells of one bacterium, classified as a cytophaga sp., hydrolytic enzymes were isolated. 3. partial purification of the enzymes is described and some of the properties of the principal enzymes have been studied. 4. the action of the enzymes on several galactan sulphates of red algae suggests that an agarase is presen ...19676056629
purification of a kappa-carrageenase from marine cytophaga species.a mixture of extracellular carrageenases was isolated from the cell-free medium of a culture of marine cytophaga sp. 1k-c783 grown on zobell 2216 e broth with 0.1% commercial carrageenan. a single active peak of kappa-carrageenase was separated and purified from the mixture by ammonium sulfate precipitation, ion-exchange chromatography, and sephadex g-200 gel filtration chromatography. molecular weight of the purified kappa-carrageenase was estimated as 100,000 by sodium dodecyl sulfate-polyacry ...19873696007
numerical taxonomy of some named bacterial cultures. 19676064342
effect of packaging on the major microbial flora of irradiated haddock. 19676067600
guidance of cytophaga sp. strain u67 gliding on the sheaths of oscillatoria princeps.individual cells of cytophaga sp. strain u67 glided in helical patterns on the surface of sheaths deposited by the cyanobacterium oscillatoria princeps. possible bases for the helical substructure of the sheath are discussed.19853921528
coaggregation of human oral cytophaga species and actinomyces israelii.a total of 19 strains of oral cytophaga sp. obtained from subgingival plaque deposits were tested for their ability to coaggregate with strains of actinomyces israelii, a. viscosus, a. naeslundii, streptococcus sanguis, s. mutans, s. salivarius, and s. mitis. coaggregation was observed only with a. israelii. based on their coaggregation patterns with eight a. israelii strains, the cytophaga strains were distributed among three distinct groups: those that coaggregated with a. israelii pk16 but no ...19836133836
studies on psychrophilic bacteria in two lakes of different trophy.the number of bacteria capable of growth at low temperatures in two lakes was found to be subject to considerable variation throughout the year although the changes were not correlated with changes in ambient temperature. a correlation was, however, observed between temperature and ratio of number of psychrophiles to "total" count. all of the psychrophilic bacteria isolated from the studied lakes were able to grow at temperatures below 0 degrees c. microorganisms isolated from eutrophic lakes we ...19816174030
studies on pectolytic bacteria in water and bottom sediments of two lakes of different trophy.pectolytic bacteria were found in greater numbers in the eutrophic than in the mesotrophic lake. however, higher pectolytic activity was exhibited by the bacteria derived from the mesotrophic lake. in the eutrophic lake higher endo- and exo-pg activity was found in the benthic than in the panktonic bacteria, whereas in the mesotrophic one higher endo-pg activity was exhibited by the planktonic and higher exo-pg activity by the benthic bacteria. the polygalacturonases synthesized by the bacteria ...19826189379
chitynolytic bacteria in water and bottom sediments of two lakes of different trophy.chitynolytic bacteria were found in greater numbers in the eutrophic lake jeziorak than in the mesotrophic lake jasne. in both lakes higher chitynolytic activity was found in the planktonic than in the benthic bacteria. the capacity of chitin hydrolysis was found in bacteria of the genera achromobacter, bacillus, nocardia, pseudomonas, of the flavobacterium-cytophaga group and of the enterobacteriaceae family. the chitinases synthesized by the studied bacteria were active in the ph range 5.0--8. ...19846209933
growth of bacteria on chitin, fungal cell walls and fungal biomass, and the effect of extracellular enzymes produced by these cultures on the antifungal activity of amphotericin b.vibrio alginolyticus, streptomyces griseus, arthrobacter g12, bacillus sp. and cytophaga sp. were grown on solid and liquid media containing soluble and insoluble carbon sources. arthrobacter g12, bacillus sp. and cytophaga sp. grew well on media which contained fungal cell walls or fungal biomass as the main carbon source. all bacteria produced extracellular proteases and all bacteria except arthrobacter g12 produced extracellular chitinases. growth of cytophaga sp. on colloidal chitin was para ...19853929028
distribution of multicopy single-stranded dna among myxobacteria and related species.multicopy single-stranded dna (msdna) is a short single-stranded linear dna originally discovered in myxococcus xanthus and subsequently found in stigmatella aurantiaca. it exists at an estimated 500 to 700 copies per chromosome (t. yee, t. furuichi, s. inouye, and m. inouye, cell 38:203-209, 1984). we found msdna in other myxobacteria, including myxococcus coralloides, cystobacter violaceus, cystobacter ferrugineus (cbfe17), nannocystis exedens, and nine independently isolated strains of m. xan ...19853932332
gliding motility in cytophaga.gliding motility in cytophaga depends on a motility machinery that keeps surfaces of cells in motion and on a surface slime that allows motile cells to translocate over a substratum. the unusual nature of the moving cell surfaces appears to be responsible for several cell-surface properties being motility dependent.19853939983
actinobacillus actinomycetemcomitans strains y4 and n27 adhere to hydroxyapatite by distinctive mechanisms.actinobacillus actinomycetemcomitans strains y4 and n27 absorb to spheroidal hydroxyapatite in roughly the same numbers per milligram of substrate and with the same tenacity as two previously tested cytophaga species. although the two strains of a. actinomycetemcomitans exhibited similar affinities and number of binding sites for sha, their response to enzyme treatment and heating were very different. the capacity of strain y4 to attach to spheroidal hydroxyapatite was diminished by treatment wi ...19853972445
potentiality of artificial sea water salts for the production of carrageenase by a marine cytophaga sp.production of an extracellular enzyme complex (carrageenase) was studied by examining cell-free fluids from cultures of a marine cytophaga, 1k-c783, growing on different media. among artificial sea water salts, only nacl and mgcl2 were utilized by the organism to produce carrageenase. the minimal concentrations of suitable combinations of nacl and mgcl2 were found to be 0.05 m nacl plus 0.25 m mgcl2, and 0.15 m nacl plus 0.15 m mgcl2. kcl and cacl2 did not have any role in carrageenase productio ...19854033467
fatty acids of myxococcus xanthus.fatty acids were extracted from saponified vegetative cells and myxospores of myxococcus xanthus and examined as the methyl esters by gas-liquid chromatography. the acids consisted mainly of c(14) to c(17) species. branched acids predominated, and iso-pentadecanoic acid constituted half or more of the mixture. the other leading component (11-28%) was found to be 11-n-hexadecenoic acid. among the unsaturated acids were two diunsaturated ones, an n-hexadecadienoic acid and an iso-heptadecadienoic ...19734197903
a bacterial factor capable of solubilizing cholinesterase from plaice body muscle. 19684305082
isolation and characterization of a new cytophaga species implicated in a work-related lung disease.a yellow-pigmented, gram-negative, gliding bacterium isolated from an industrial water spray air humidification system was implicated as a causative agent in several occurrences of lung disease with hypersensitivity pneumonitis-like symptoms. the bacterium, designated wf-164, lacked microcysts or fruiting bodies and had a dna base composition of 34.8 mol% of guanine plus cytosine. gliding, flexing, nonflagellated cells measuring 0.3 by 3.5 to 8.9 micron were observed by using light and electron ...19846508308
evidence for the participation of n-acetylated amino sugars in the coaggregation between cytophaga species strain dr2001 and actinomyces israelii pk16.coaggregation between cytophaga sp. strain dr2001 and actinomyces israelii pk16 was partially inhibited by certain n-acetylated amino sugars (n-acetylneuraminic acid, n-acetylgalactosamine, and n-acetylglucosamine) and was completely inhibited by the trisaccharide neuraminin-lactose. the monosaccharides exerted their effect at concentrations between 30 to 100 mm, whereas the trisaccharide was an effective inhibitor at significantly lower concentrations. outer membrane preparations caused a. isra ...19846715035
formation of yeast protoplasts by using an enzyme preparation from cytophaga. 19714335688
effect of amendments on the microbial utilization of oil applied to soil.replicate field plots comprising a control, plus oil, plus oil and bacteria, plus oil and fertilizer (urea-phosphate; 27:27:0), and plus oil, bacteria, and fertilizer were monitored over a 308-day period for changes in bacterial and mold numbers. changes in the chemical composition of the oil applied to the plots was followed by using chromatographic techniques. application of fertilizer resulted in a stimulation of bacterial numbers and in the rate of utilization of n-alkane components of the s ...19744589125
characterization of the -d-glucan from the plastids of cecropia peltata as a glycogen-type polysaccharide. 19734738054
deoxyribonucleic acid homology in bacterial taxonomy: effect of incubation temperature on reaction specificity.parameters affecting deoxyribonucleic acid duplex (dna-dna) formation on membrane filters were evaluated. the reference strains used were cytophaga succinicans strain 8, which has a guanine plus cytosine (gc) content of 38%, and myxococcus xanthus strain fb, which has a gc content of 70%. both organisms are gliding bacteria classified among the myxobacteria. among the parameters evaluated, the incubation temperature used during duplex formation was found to be the most important in terms of the ...19684966832
breakdown of chitin by cytophaga johnsonii. 19725073232
deoxyribonucleic acid relatedness of some menaquinone-producing flavobacterium and cytophaga strains.nine menaquinone-forming strains of the flavobacterium--cytophaga complex with dna base compositions between 35 and 45 moles percent guanine-plus-cytosine were investigated for genome sizes and dna relatedness by dna:dna hybridization in vitro, using the optically recorded initial reassociation kinetics. two strains representing c. hutchinsonii and c. marinoflava proved to be related on the 50 percent binding level, i.e. on a level of dna relatedness commonly found within well-classified convent ...19807396480
development of an rrna-targeted oligonucleotide probe specific for the genus acinetobacter and its application for in situ monitoring in activated sludge.enhanced biological phosphate removal in an anaerobic-aerobic activated sludge system has generally been ascribed to members of the genus acinetobacter. a genus-specific 16s rrna-targeted oligonucleotide probe was developed to investigate the role of acinetobacter spp. in situ. nonisotopic dot blot hybridization to 66 reference strains, including the seven described acinetobacter spp., demonstrated the expected probe specificity. fluorescent derivatives were used for in situ monitoring of acinet ...19947512807
[myxobacterial type quotient as an eutrophy indicator in surface waters]. 19655323597
bacterial community structures of phosphate-removing and non-phosphate-removing activated sludges from sequencing batch reactors.the bacterial community structures of phosphate- and non-phosphate-removing activated sludges were compared. sludge samples were obtained from two sequencing batch reactors (sbrs), and 16s rdna clone libraries of the bacterial sludge populations were established. community structures were determined by phylogenetic analyses of 97 and 92 partial clone sequences from sbr1 (phosphate-removing sludge) and sbr2 (non-phosphate-removing sludge), respectively. for both sludges, the predominant bacterial ...19957544094
recovery of uncommon bacteria from blood: association with neoplastic disease.table 6 is a summary of the organisms discussed with a listing of the environmental source, the endogenous source, the predisposing factors including neoplasms, and the postulated mechanisms by which the organism can gain access to the circulation. the evidence considered indicates that the entrance of one of these microorganisms into the bloodstream of a human being depends on the presence of multiplicity of predisposing factors. in the majority of cases of bacteremia due to one of these unusua ...19957553569
biodiversity of gas vacuolate bacteria from antarctic sea ice and water.psychrophilic, gas vacuolate, heterotrophic bacteria indigenous to sea ice communities in antarctica have been isolated. phylogenetic analysis of representative members of these bacteria shows that they belong to the alpha, beta, and gamma proteobacteria and the flavobacteria-cytophaga group. this is the first report of gas vacuolate bacteria from the beta proteobacteria and the flavobacteria-cytophaga groups.19957574660
sulfobacins a and b, novel von willebrand factor receptor antagonists. ii. structural elucidation.sulfobacins a and b are novel von willebrand factor (vwf) receptor antagonists produced by chryseobacterium sp. nr 2993. the structures of sulfobacins a and b have been determined to be (2r,3r)-3-hydroxy-2-[(r)-3-hydroxy-15-methylhexadecanamido]-15- methylhexadecanesulfonic acid and (2r,3r)-3-hydroxy-15-methyl-2-[13-methyltetradecanamido]- hexadecanesulfonic acid, respectively, by various 2d nmr experiments and by methanolysis. the absolute configurations of the sulfobacins were determined by a ...19957592057
[the level of dna homology in species of the genus cytophaga].nucleotide composition, genome size and dna homologies were studied for strains cytophaga johnsonae dsm 425, c. pectinovora dsm 6368, c. saccharophyla dsm 1811, c. aquatilis dsm 2063, c. flevensis dsm 1076, c. lytica atcc 23 178, c. arvensicola dsm 3695, c. heparina dsm 2356. a considerable difference in gs-bases level between species of genus cytophaga (33.3-34.9 mol %) and incertae sedis species (c. heparina and c. arvensicola) (45.0-46.7 mol. %) was shown. the differentiation between freshwat ...19957655657
phylogenetic diversity of the genus cytophaga revealed by 16s rrna sequencing and menaquinone analysis.to clarify the intra- and intergeneric relationships of the genus cytophaga, 16s rrna sequences and respiratory isoprenoid quinones were determined for the type strains of the 21 validly published species and one isolate in the genus cytophaga. the sequence analysis revealed extreme heterogeneity of this genus, which diverged into nine distinct lines of descent. each lineage of cytophaga was characterized by possessing either menaquinone-6 (mk-6) or mk-7. the mk-6-possessing species were located ...19937689634
the specificity of an agarase from a cytophaga species.1. the extracellular agarase from a cytophaga species was shown to have no action on neoagarobiose, neoagarotetraose or their analogues containing 6-o-methyl-d-galactose residues. 2. the action of the enzyme on neoagaro-octaose suggests that scission of the central beta-d-galactosidic linkage, to form two molecules of tetrasaccharide, is the preferred mode of action; however, both exterior d-galactosidic linkages in the octasaccharide and both in neoagarohexaose are hydrolysed at a somewhat lowe ...19695386191
cis-11-hexadecenoic acid from cytophaga hutchinsonii lipids. 19695773100
hydrogen bonding of sulfur ligands in blue copper and iron-sulfur proteins: detection by resonance raman spectroscopy.the resonance raman spectrum of the blue copper protein azurin from alcaligenes denitrificans exhibits nine vibrational modes between 330 and 460 cm-1, seven of which shift 0.4-3.0 cm-1 to lower energy after incubation of the protein in d2o. these deuterium-dependent shifts have been previously ascribed to exchangeable protons on imidazole ligands [nestor, l., larrabee, j. a., woolery, g., reinhammar, b., & spiro, t. g. (1984) biochemistry 23, 1084] or to exchangeable protons on amide groups whi ...19873442645
growth regulation of the b lymphoma cell line wehi-231 by anti-immunoglobulin, lipopolysaccharide, and other bacterial products.the growth of wehi-231, a murine immature b lymphoma cell line, was inhibited by anti-igm antibodies. the inhibition of proliferation, as measured by [3h]thymidine incorporation, occurred between 16 and 28 hr after addition of anti-igm. moreover, the growth arrest was irreversible: cells that were cultured with anti-igm for 18 hr and then recultured without it failed to recover the ability to proliferate, even though cells treated for up to 30 hr with anti-igm remained viable, as measured by try ...19863489760
induction of human immunoglobulin synthesis (igg, iga) by the novel, t cell independent mitogen cytophaga allerginae endotoxin.cytophaga allerginae endotoxin (cae) has been purified from c. allerginae, a newly discovered bacterial species isolated from a chilled water spray humidification system. the present study was undertaken in order to determine whether cae can induce immunoglobulin synthesis by human peripheral blood lymphocytes (pbl) in culture. to this end, human pbl were purified and cultured with either pokeweed mitogen at 5 micrograms/ml, or cae (at varying concentrations) for 6 days. the levels of igg and ig ...19873497882
fine structure and host-virus relationship of a marine bacterium and its bacteriophage.valentine, artrice f. (georgetown university, washington, d.c.), and george b. chapman. fine structure and host-virus relationship of a marine bacterium and its bacteriophage. j. bacteriol. 92:1535-1554. 1966.-the fine structure of a gram-negative marine bacterium, cytophaga marinoflava sp. n., has been revealed by ultrathin sectioning and electron microscopy. stages in the morphogenesis of the bacterial virus ncmb 385, which has been shown to be highly specific for this organism, were also demo ...19665924277
phylogeny of bacteroides, prevotella, and porphyromonas spp. and related bacteria.the phylogenetic structure of the bacteroides subgroup of the cytophaga-flavobacter-bacteroides (cfb) phylum was examined by 16s rrna sequence comparative analysis. approximately 95% of the 16s rrna sequence was determined for 36 representative strains of species of prevotella, bacteroides, and porphyromonas and related species by a modified sanger sequencing method. a phylogenetic tree was constructed from a corrected distance matrix by the neighbor-joining method, and the reliability of tree b ...19948300528
properties of two marine bacteriophages.chen, peter k. (georgetown university, washington, d.c.), ronald v. citarella, omar salazar, and rita r. colwell. properties of two marine bacteriophages. j. bacteriol. 91:1136-1139. 1966.-various properties have been determined for two bacteriophages, ncmb 384 and 385, and their host, ncmb 397, a cytophaga sp., isolated from the marine environment. the purified bacteriophages have been subjected to serological analysis, results of which indicate a high degree of relatedness. purified, highly po ...19665929746
tan-1057 a-d, new antibiotics with potent antibacterial activity against methicillin-resistant staphylococcus aureus. taxonomy, fermentation and biological activity.two gram-negative bacteria were found to produce the new antibacterial antibiotics tan-1057 a, b, c and d. the producing bacteria were characterized and designated as flexibacter sp. pk-74 and pk-176. these antibiotics were active against gram-negative and gram-positive bacteria, including methicillin-resistant staphylococcus aureus. tan-1057 a inhibited protein biosynthesis in escherichia coli and s. aureus. it showed excellent protective effects against an experimental methicillin-resistant s. ...19938501003
purification, characterization and specificity of chondroitin lyases and glycuronidase from flavobacterium heparinum.the chondroitin lyases from flavobacterium heparinum (cytophaga heparinia) have been widely used in depolymerization of glycosaminoglycan and proteoglycan chondroitin sulphates. oligosaccharide products derived from chondroitin sulphate can be further degraded by glycuronidases and sulphatases obtained from the same organism. there has been no reported purification of these enzymes to homogeneity nor is there any information on their physical and kinetic characteristics. the absence of pure enzy ...19958526872
the enzymic degradation of porphyran.1. the algal galactan, porphyran, was incubated with enzymes from a cytophaga sp. and the products were examined. 2. only about 30% of the porphyran was recovered in the form of oligosaccharides, the remainder being of high molecular weight. 3. among the saccharides were d-galactose, 6-o-methyl-d-galactose, neoagarobiose, neoagarotetraose and oligosaccharides containing 6-o-methyl-d-galactose, the principal of which has been tentatively identified as 6(3)-o-methyl-neoagarotetraose. fragments con ...19676060448
development of techniques for the genetic manipulation of the gliding bacterium cytophaga johnsonae.cytophaga johnsonae displays many features that make it an excellent model of bacterial gliding motility. unfortunately, genetic analyses of c. johnsonae, or any related gliding bacteria, were not possible because of a complete lack of selectable markers, cloning vectors, transposons, and convenient methods of gene transfer. as a first step toward a molecular analysis of gliding motility of c. johnsonae, we developed these genetic techniques and tools. common broad-host-range plasmids and transp ...19968550486
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