| activation of tumoricidal properties in mouse macrophages by lymphokines encapsulated in liposomes. | cell-free culture supernatants rich in macrophage-activating factor (maf) activity obtained from mitogen-stimulated f344 rat lymphocytes have been encapsulated within liposomes of differing size and lipid composition and their ability to render normal mouse macrophages cytotoxic for tumor cells in vitro has been compared with that of unencapsulated (free) maf added to the extracellular medium. normal macrophages from c57bl/6, c3h/hen, and c57bl/6 x c3h f1 mice treated with liposome-encapsulated ... | 1979 | 427777 |
| studies on conformational changes in f-actin of glycerinated muscle fibers during relaxation by means of polarized ultraviolet fluorescence microscopy. | by means of polarized ultraviolet fluorescence microscopy the conformational changes of f-actin occuring in glycerinated muscle fibers of rabbit and barnacle (balanus rostratus hock.) under the influence of adenosine triphosphate in the presence of ethylene glycol bis(beta-amino-ethyl ether)-n,n'-tetraacetic acid were discovered. these changes seem to be located near the surface of the globules thus hampering the penetration of univalent iones and neutral molecules into the f-actin macromolecule ... | 1979 | 470613 |
| origin and early development of canine circumanal glands. | | 1979 | 517821 |
| time-course of changes in baroreflex sensitivity during the development of renovascular hypertension in rats [proceedings]. | | 1979 | 529094 |
| effects of neonatal undernutrition on small intestinal dipeptidase(s) activity. | | 1979 | 546746 |
| [electron microscopical evidence of spiroplasma citri in nucleus of infected testplants (author's transl)]. | this is the first report for the evidence of mycoplasmas in an organelle of a plant cell. spiroplasma citri was detected by electron microscopy in the nucleus of a phloem parenchyma cell of chrysanthemum carinatum schousboe plants, inoculated experimentally by euscelis plebejus fall. | 1979 | 549392 |
| pharmacological and toxicological studies with 1-benzyl-3-(2,3-dihydroxypropoxy)indazole. | 1-benzyl-3-(2,3-dihydroxypropoxy)-indazole (benzidol) is a novel indazolane derivative with marked antiinflammatory, analgesic and antipyretic properties. its acute toxicity is three times less than that of benzidamine and five times less than that of phenylbutazone. rats, mice, guinea pigs and rabbits treated with benzidol during 6 months at doses of 5, 50 and 200 mg/kg did not present any pathological alteration. the anti-inflammatory activity of benzidol is similar to that of phenylbutazone a ... | 1976 | 1087562 |
| polymorphism of human color vision. | the genetic polymorphism of human color vision is examined within the framework of a photopigment replacement model. an analysis of the x-linked recessive dichromacies and anomalous trichromacies indicates that one source of variability of normal color perception may be the inclusion of several distinct phenotypes in what is usually described as normal color vision. this analysis also reveals the cause of the dominance hierarchy at the protan and deutan loci, the perceptual effects of dosage com ... | 1976 | 1087832 |
| endogenous prostaglandin release contributes directly to coronary artery tone. | an in vitro coronary artery preparation of beef heart was found to synthesize and release continuously large amounts of a prostaglandin of the e type. inhibition of prostaglandin synthesis with aspirin, indomethacin, or eicosa-5,8,11,14-tetraynoic acid induced a sustained contraction of the coronary artery, and pretreatment with indomethacin diminished markedly the output of prostaglandin into the bathing medium. it appears that prostaglandin e1, generated from within the vessel wall itself, may ... | 1975 | 1175084 |
| [effect of anoxia and defibrillation on the synthesis of proteins of extracorporally connected dog's heart perfused with the aid of a homologous donor]. | | 1975 | 1183371 |
| attenuated, streptomycin-dependent salmonella typhi oral vaccine: potential deleterious effects of lyophilization. | four studies were done with streptomycin-dependent salmonella typhi as an oral, attenuated vaccine. studies 1 and 3 employed freshly harvested vaccine, whereas studies 2 and 4 involved lyophilized vaccine. five to eight doses (3 x 10(10)-10(11) organisms/dose) were given; oral streptomycin (1.0 g) was administered concomitantly in studies 2 and 3, with only two of the doses of vaccine in study 1, and was not given in study 4. no adverse reactions were encountered in 179 vaccinated men, and 94% o ... | 1976 | 1262709 |
| gill parasites of the spot leiostomus xanthurus from clear lake, texas. | | 1976 | 1265958 |
| species difference in toxicoloty. | | 1976 | 1265977 |
| [effect of tricyclic antidepressive agents on hemodynamics and plasms norepinephrine level in man during rest and exertion]. | the effects of i.v. injected antidepressants imipramine (tofranil, geigy) and dimetacrine (istonil, siegfried) on cardiavascular functions were investigated in 18 patients at diagnostic cardiac catheterization. 4 patients were additionally studied during ergometric exercise before and 45 min. after application of both thymoleptica. determinations of noradrenaline plasma concentrations were carried out in 8 patients. the results show of 25 mg (= 22,1 mg free base) imipramine or 33.5 mg (= 21.4 mg ... | 1976 | 1266277 |
| [test of degranulation of basophils and mast cells for detection of allergic diseases (literature survey)]. | | 1976 | 1274295 |
| tuberculosis in patients infected with human immunodeficiency virus 1. a retrospective multicentre study of 123 cases in france. the groupe des infectiologues du sud de la france. | in order to study the epidemiological, clinical, and progressive characteristics of tb in hiv-infected individuals, a retrospective study was conducted in nine infectious disease centres of university hospitals located in the southern half of france. among the 5730 hiv-seropositive in- and out-patients, 123 (2.1 per cent) had tb (121 infections caused by m. tuberculosis, 2 by m. bovis). tuberculosis was pulmonary in 53 patients (43.1 per cent), extrapulmonary in 36 patients (29.3 per cent), and ... | 1992 | 1287704 |
| expression and release of neuroregulators during development: monoamines and neuropeptides. | the turnover of catecholamines (ca) was determined in the adrenal medulla and brain of rat fetuses and pups. in general we found a considerable increase soon after birth. the expression of mrna for ca-synthesizing enzymes was also considerably enhanced in the adrenals shortly after birth. furthermore, we demonstrated increased expression of neuropeptides after birth, increased synthesis of mrna encoding for neuropeptide y in the adrenals 24 h after birth; and considerable activation of the subst ... | 1992 | 1306802 |
| opioid peptides reduce synaptic transmission in the nucleus accumbens. | behavioral studies implicate the nucleus accumbens (nacc) as a brain area pivotal for the rewarding effects of opiates like heroine and morphine. therefore, we studied the effect of a variety of opioids on membrane properties and responses to synaptic stimulation in a slice preparation of the nacc using intracellular recording. superfusion of opioid peptides did not affect the membrane potential or input resistance of nacc neurons, but significantly reduced both depolarizing and hyperpolarizing ... | 1992 | 1317025 |
| kappa-opioid receptor stimulation abolishes mu- but not delta-mediated inhibitory control of spinal met-enkephalin release. | the possible opioid control through delta, mu and kappa receptors of the spinal release of met-enkephalin-like material (melm) was investigated in halothane-anaesthetized rats. the intrathecal perfusion of the delta agonist dtlet (10 microm) or the mu agonist dago (10 microm) resulted in a marked inhibition of melm release, which could be prevented by the selective antagonists naltrindole and naloxone, respectively. although the kappa agonist u 50488 h (10 microm) was inactive per se, it complet ... | 1992 | 1317026 |
| human papillomavirus (hpv) types specific of epidermodysplasia verruciformis detected in warts induced by hpv3 or hpv3-related types in immunosuppressed patients. | epidermodysplasia verruciformis (ev) is characterized by an abnormal genetic predisposition to infection with specific types of human papillomavirus (hpv). specific defects of the cell-mediated immunity and/or of the control of hpv infection in keratinocytes are assumed to be involved in the development of the disease. as a model to test this hypothesis, we have studied the prevalence of ev-specific hpv in skin warts of 56 immunosuppressed patients. all main types of cutaneous hpv (hpv1, 2, 3, 4 ... | 1992 | 1317396 |
| cytomegalovirus and pneumocystis carinii pneumonia in children with acquired immunodeficiency syndrome. | to ascertain the effect of cytomegalovirus (cmv) infection on the course of pneumocystis carinii pneumonia (pcp) in children with acquired immunodeficiency syndrome (aids), we reviewed the charts of all children with aids who also had a lung biopsy specimen or a bronchoalveolar lavage specimen cultured for cmv at the time pcp was diagnosed. the data indicate that children with aids and pcp whose cultures are positive for cmv do not have a poorer prognosis during a first episode of pcp compared w ... | 1992 | 1317421 |
| conditioned place preference induced by ro 16-6028, a benzodiazepine receptor partial agonist. | a place conditioning situation was used to assess the putative affective properties of benzodiazepine receptor ligands in the rat. the benzodiazepine receptor partial agonist ro 16-6028 induced a conditioned place preference, suggesting that this compound has rewarding properties. the benzodiazepine receptor antagonist ro 15-1788 induced neither place preference nor aversion, but prevented the place preference induced by ro 16-6028, suggesting that the rewarding effects of ro 16-6028 are due to ... | 1992 | 1317585 |
| the metabolism of dup 753, a nonpeptide angiotensin ii receptor antagonist, by rat, monkey, and human liver slices. | the in vitro metabolism of dup 753, a novel nonpeptide angiotensin ii receptor antagonist, has been investigated in incubations with liver slice preparations from rats, monkeys and humans. metabolites were identified by hplc/ms, fab/ms, cl/ms, and/or 1h nmr. in the rat, the primary route of metabolism was oxidative, leading to either monohydroxylated or oxidized (carboxylic acid) metabolites, whereas in monkeys, glucuronidation of the tetrazole moiety predominated. an equal mixture of both oxidi ... | 1992 | 1352222 |
| influence of low-temperature storage and glucose starvation on growth recovery in escherichia coli rela and rela+ strains. | to study the influence of microgravity on bacterial growth behavior during a space mission, the special experimental conditions and the hardware environment necessitate storage of cells at low temperature, and permit a relatively short experimental period. before this experimental period, cells have to recover their condition of steady-state growth, because it is only in this condition that the growth behavior of the flight and ground populations can be adequately compared. to meet these require ... | 1991 | 1367237 |
| characterization of guanosine diphospho-d-mannose dehydrogenase from pseudomonas aeruginosa. structural analysis by limited proteolysis. | alginate is believed to be a major virulence factor in the pathogenicity of pseudomonas aeruginosa in the lungs of patients suffering from cystic fibrosis. guanosine diphospho-d-mannose dehydrogenase (gdpmannose dehydrogenase, ec 1.1.1.132) is a key enzyme in the alginate biosynthetic pathway which catalyzes the oxidation of guanosine diphospho-d-mannose (gdp-d-mannose) to gdp-d-mannuronic acid. in this paper, we report the structural analysis of gmd by limited proteolysis using three different ... | 1992 | 1370473 |
| termination of second messenger signaling in olfaction. | by using isolated rat olfactory cilia and a fast kinetics methodology, it has been demonstrated that odorant-induced second messenger signaling in the millisecond time range is terminated via phosphorylation reactions catalyzed by specific protein kinases. the cyclic adenosine nucleotide pathway is turned off by kinase a activity, whereas the inositol trisphosphate cascade is terminated by kinase c. the data support the concept that desensitization of odorant responses involves phosphorylation o ... | 1992 | 1370581 |
| thallium-201 accumulation by epidermoid inclusion cyst. | an elderly male undergoing re-evaluation for coronary artery disease demonstrated an extra-cardiac focus of 201tl accumulation during the performance of a planar myocardial perfusion scan. this corresponded to a subcutaneous lesion of the left posterior thorax found on a concurrent computerized axial tomographic (cat) scan and upon surgical excision proved to be an "epidermoid inclusion cyst." a follow-up nuclear scan failed to reveal any residual extracardiac tracer localization. confusing this ... | 1992 | 1403158 |
| [a case from practice (257). cryptococcus meningitis in aids (group ivc1, center for disease control)]. | | 1992 | 1448637 |
| molecular correlates of physiological regulation in a p-type h(+)-atpase. | | 1992 | 1449060 |
| fibroblastic regulation of osteoblast function by prostaglandins. | the effects of osteogenic inhibitory factors secreted by human periodontal ligament fibroblasts were studied in rat bone marrow stromal cell cultures. serum-free conditioned medium from cultures of fibroblasts strongly depressed formation of mineralized tissue by bone marrow cell cultures. the inhibitory activity was reduced by treatment of fibroblast cultures with indomethacin or by pretreatment of conditioned medium with specific antibodies to prostaglandins (pgs) e2 and f2 alpha. passage of c ... | 1992 | 1489657 |
| characterization of endogenous substrates for novel-type protein kinase c as well as conventional-type protein kinase c in primary cultured mouse epidermal cells. | in primary cultured mouse epidermal cells, phorbol 12-myristate 13-acetate (pma), which activates protein kinase c (pkc), induced changes in the phosphorylation levels of 10 proteins, termed kp-1 to -10, in two-dimensional page. seven of these proteins were phosphorylated and three were dephosphorylated. similar changes were induced by other pkc activators, but not by inactive phorbol ester. among these substrate proteins, phosphorylation of three proteins, i.e. kp-1 (pi 4.7/23,000 m(r)), kp-2 ( ... | 1992 | 1489665 |
| localization and quantification of nonapeptide binding sites in the kidney of xenopus laevis: evidence for the existence of two different nonapeptide receptors. | the distribution and properties of nonapeptide binding sites in the kidney of the anuran xenopus laevis were investigated using quantitative in vitro autoradiography. the binding studies were performed with [3h]arginine vasopressin (avp) as ligand because [125i]arginine vasotocin (avt) lacks biological activity. specific binding sites for [3h]avp are located in the glomeruli of the kidney. [3h]avp binding results in a steady state of association and dissociation between ligand and binding sites. ... | 1992 | 1563620 |
| the no-reflow phenomenon is a post-mortem artifact. | post-ischemic reperfusion impairment, ("no-reflow phenomenon"), was studied in rats subjected to 8-30 minutes of global brain ischemia. during ischemia, rapid and complete loss of cerebral blood flow, eeg and 31p-high energy phosphates (atp/pcr) was observed. brain intravascular perfusion defects were examined by injecting carbon black intravenously in a group of rats with stable cardiopulmonary function and in another group subjected to rapid thoracotomy and intraarterial infusion of the carbon ... | 1992 | 1595393 |
| [cryptococcosis in aids patients: a study of 19 cases]. | we discuss 19 cases of infection due to cryptococcus neoformans diagnosed in 438 aids patients admitted to our center (4%). fourteen of them showed meningitis confirmed by culture of c. neoformans in csf. clinical features were rather unspecific and disorders in csf parameters were non striking. the diagnostic techniques performed with best results were culture of c. neoformans and antigen determination, especially in serum. survival probability at one year was 75%. treatment response was good. ... | 1992 | 1626090 |
| altered peripheral benzodiazepine receptor density in human granulosa-lutein cells in relation to follicular maturity. | since the introduction of treatment by in vitro fertilization/embryo transfer (ivf-et) in humans, a harmless approach to identifying the ovum with a greater chance of producing an implantable embryo has been sought worldwide. our previous studies indicated a high correlation between degree of follicular maturation and benzodiazepine (bz) receptor density in rats. we hypothesized that if such a correlation existed in humans, the latter might be used to evaluate degree of follicular maturation and ... | 1991 | 1665455 |
| terminal epidermal differentiation of human keratinocytes grown in chemically defined medium on inert filter substrates at the air-liquid interface. | a fully differentiated epithelium having the features of epidermis was obtained in vitro by culturing second-passage normal human keratinocytes (nhk) in the chemically defined medium mcdb 153 on inert filter substrates at the air-liquid interface for 14 d. vertical sections stained for histology and indirect immunofluorescence studies show a correct stratification and expression of differentiation markers. the presence of desmosomes, keratohyalin granules, and lamellar granules, and the formatio ... | 1990 | 1698886 |
| analysis of high psa n-cam expression during mammalian spinal cord and peripheral nervous system development. | using a monoclonal antibody that recognizes specifically a high polysialylated form of n-cam (high psa n-cam), the temporal and spatial expression of this molecule was studied in developing spinal cord and neural crest derivatives of mouse truncal region. temporal expression was analyzed on immunoblots of spinal cord and dorsal root ganglia (drgs) extracts microdissected at different developmental stages. analysis of the ratio of high psa n-cam to total n-cam indicated that sialylation and desia ... | 1991 | 1769342 |
| structural and biochemical changes in the sclera of experimentally myopic eyes. | | 1991 | 1794573 |
| the change in the threshold for short-term desensitization in isolated smooth muscle cells showing an all-or-none response to acetylcholine. | 1. isolated smooth muscle cells from guinea-pig taenia caecum were prepared by collagenase digestion. the cells showed an all-or-none response to acetylcholine (ach) under our experimental conditions. 2. desensitized cells showed an all-or-none response but required a higher concentration of ach for induction of contraction, indicating that the desensitization was due to a change in the threshold concentration. 3. in [3h]-quinuclidinyl benzilate ([3h]-qnb) binding to the desensitized cells, kd a ... | 1991 | 1797324 |
| the change in the threshold for short-term desensitization in isolated smooth muscle cells showing an all-or-none response to acetylcholine. | 1. isolated smooth muscle cells from guinea-pig taenia caecum were prepared by collagenase digestion. the cells showed an all-or-none response to acetylcholine (ach) under our experimental conditions. 2. desensitized cells showed an all-or-none response but required a higher concentration of ach for induction of contraction, indicating that the desensitization was due to a change in the threshold concentration. 3. in [3h]-quinuclidinyl benzilate ([3h]-qnb) binding to the desensitized cells, kd a ... | 1991 | 1797324 |
| epidermal growth factor exerts neuronotrophic effects on dopaminergic and gabaergic cns neurons: comparison with basic fibroblast growth factor. | basic fibroblast growth factor (bfgf) and epidermal growth factor (egf) have been described to exert neuronotrophic effects on central nervous system neurons in culture. to study the selectivity of trophic actions of these growth factors, neurotransmitter-identified populations of embryonic rat mesencephalon were used. at 20 days in vitro, egf (3 ng/ml) promoted survival and neurite outgrowth from these neurons. the neuritogenic effect of bfgf (3 ng/ml) was, however, more robust. quantitative an ... | 1991 | 1800771 |
| the role of c5 and t-cell receptor vb genes in susceptibility to collagen-induced arthritis. | collagen-induced arthritis (cia) is a rodent arthritis model in which immunization with heterologous type ii collagen induces an inflammatory polyarthritis. susceptibility to the disease is mediated by major histocompatibility complex (mhc) genes as well as genes at other loci. previous studies of the swr/j mouse strain, which is resistant to cia despite bearing the susceptible h-2q haplotype, have suggested that this resistance is the result of a deletion of t-cell receptor (tcr) vb gene segmen ... | 1991 | 1855815 |
| relevance of n-nitrosamines to oesophageal cancer in china. | oesophageal cancer occurs at a very high frequency in certain areas of china, especially in linxian county, henan province. previous studies suggested that n-nitroso compounds play a causative role. in order to study further the exposure of linxian inhabitants, the intake of n-nitrosamines in the diet was determined. the total daily intake of volatile nitrosamines and of n-nitroso-n-methylbenzylamine (nmbza) was higher in linxian than in two other counties. nmbza can induce cancer in animal and ... | 1991 | 1855832 |
| role of oncogenes and tumour suppressor genes in human lung carcinogenesis. | six families of activated protooncogenes, ras, raf, fur, neu, jun and myc have so far been associated with human lung cancer. human bronchial epithelial cells in vitro are being used to investigate the functional role of these specific oncogenes and growth regulatory genes in carcinogenesis and tumour progression. when transferred into normal human bronchial epithelial cells by the highly efficient protoplast fusion method, the v-ha-ras oncogene initiates a cascade of events leading to decreased ... | 1991 | 1855868 |
| spontaneous bacterial peritonitis in a healthy male caused by streptococcus viridans. | | 1991 | 1913382 |
| pneumatic antishock garment decreases hemorrhage and mortality from splenic injury. | the effect of the pneumatic antishock garment (pasg) in controlling hemorrhage and death from splenic injury was studied in a canine model. twelve (two groups of 6) anesthetized dogs had their spleens crushed. carotid blood pressure, carotid blood flow, splenic artery flow and abdominal aortic flow, as well as the death rate and blood loss, were measured. group 1 dogs did not have pasg inflation, but group 2 dogs had pasg inflation to an intraperitoneal pressure of 60 mm hg. all group 1 dogs die ... | 1991 | 1913398 |
| [canonical discriminant analysis for hematological and serum biochemical changes during pregnancy period in squirrel monkeys (saimiri sciurea)]. | hematological and serum biochemical data obtained from non-pregnant, pregnant and post-partum squirrel monkeys (saimiri sciurea) were analyzed by canonical discriminant analysis (discriminant analysis with reduction of dimensionality). all animals were of wild origin and had been maintained under uniform environmental conditions at tsukuba primate center for medical science, n.i.h., japan. months were standardized by the day of parturition. the calculated arithmetic means and standard deviations ... | 1991 | 1915598 |
| [creatinine clearance as the foundation for clinical determination of kidney function in swine]. | comparative examinations of renal function using inulin- and endogenous creatinine-clearance tests in 5 pigs showed nearly identical values of glomerular filtration rate (gfr). based on this relation 79 healthy pigs, weighting 2 kg to 230 kg were investigated using the creatinine-clearance test for determination of normal values of gfr and urine flow rate (vu) as well as renal excretion (e), renal clearance (clr) and fractional excretion (fe) of urea, sodium, potassium, calcium, phosphorus, gluc ... | 1991 | 1948984 |
| cytolytic activity in t cell clones derived from human synovial rheumatoid membrane: inhibition by synovial fluid. | a panel of t cell clones was derived from the synovial membrane of a patient with rheumatoid arthritis (ra). we investigated whether t cell clones with cytolytic properties were present and whether t cell cytotoxicity was influenced by the presence of synovial fluid. these issues were studied using anti-cd3 and lectin-induced cytotoxicity assays. the majority of the t cell clones derived from the synovial membrane showed cytotoxic properties although non-cytotoxic clones were also found. three c ... | 1990 | 2148285 |
| protective effect of inactivated pasteurella haemolytica bacterin challenged in bovine herpesvirus-1 experimentally infected calves. | formalin-inactivated and sonicated pasteurella haemolytica bacterins were prepared from 1 h cultures of bacterial pellets in rpmi-1640 medium containing 7% fetal calf serum. the bacterial pellets were obtained from logarithmic phase growth of the organism by centrifugation. the protective effect of the vaccine was evaluated in 43 specific-pathogen-free hereford crossbred calves and yearlings in three experiments. cattle were either single vaccinated or boosted via three routes; intratracheally ( ... | 1990 | 2168606 |
| axoplasmic electrolyte contents measured by x-ray microanalysis in experimental uremic neuropathy. | we investigated the mechanism of uremic neuropathy using experimental acute and chronic renal failure models in rats. after induction of renal failure, the motor nerve conduction velocity, axoplasmic electrolyte content, and cross-sectional area of myelinated fibers in the sciatic nerve were determined. the axoplasmic na, k, cl, and ca contents were measured by electron probe x-ray microanalysis employing with sections from freshly freeze-dried sciatic nerves. the results obtained for both the a ... | 1990 | 2174479 |
| abelson murine leukemia virus transforms preneoplastic emu-myc transgene-carrying cells of the b-lymphocyte lineage into plasmablastic tumors. | e mu-myc transgenic mice were back-crossed to balb/c mice up to back-cross generation 3. the offspring that included transgene-carrying and -negative mice in approximately equal proportions were randomly divided into 2 groups. thirty-four mice (group i) were treated with pristane, followed by a-mulv, and 40 (group ii) were injected with a-mulv alone. altogether, 16 lymphoid tumors developed in group i and 17 in group ii. nine of the tumors in group i and 4 in group ii appeared as ascitic tumors. ... | 1990 | 2228313 |
| staphylococcus aureus infection of human endothelial cells potentiates fc receptor expression. | vasculitis, a recognized complication of staphylococcal-endovascular infections, may result in part, from the expression of fcr by staphylococcus aureus-infected endothelial cells. fcr were measured using [51]cr labeled srbc preincubated with rabbit anti-srbc igg. fcr were not detected on uninfected endothelial cells, but were demonstrated on s. aureus infected cells using igg, but not igm labeled srbc. fcr expression was dependent on the initial bacterial density (greater than or equal to 8 x 1 ... | 1990 | 2258617 |
| structures of the asparagine-linked oligosaccharides of guinea-pig factor b of the alternative complement pathway. | this paper describes the structures of the asparagine-linked oligosaccharides of two forms of guinea-pig factor b of the alternative complement pathway with different mr values. oligosaccharides were quantitatively liberated from both glycoproteins by hydrazinolysis, fractionated by paper electrophoresis and bio-gel p-4 column chromatography, and their structures determined by sequential exoglycosidase digestions in conjunction with methylation analysis. both glycoproteins were shown to have the ... | 1990 | 2268280 |
| induction of carcinoembryonic-antigen-gene expression in human colorectal carcinoma by sodium butyrate. | the effect of sodium butyrate on the expression of the carcinoembryonic-antigen (cea) gene was studied in two poorly differentiated colorectal-carcinoma cell lines (clone-a and mip-101) and in one well-differentiated cell line (ls-174t); a.t.c.c. no. ccl 188). northern-blot and dot-blot analyses indicated a steady increase in cea mrna from day 4 to a maximal level by day 14 after these cells were exposed to 2 mm-sodium butyrate. studies using nuclear run-off assays followed by dot-blot hybridiza ... | 1990 | 2268282 |
| heat sensitivity and protein synthesis during heat-shock in the tobacco hornworm, manduca sexta. | fifth instar larvae of the tobacco hornworm, manduca sexta, tolerate 1-h exposures to temperatures as high as 42 degrees c. above 42 degrees c, survival declines rapidly to 18% at 44 degrees c and 0% at 48 degrees c. as in other insects, the heat-shock response of manduca sexta involves the induction of synthesis of heat-shock proteins very similar in size to the drosophila heat-shock proteins (84, 73, 71, 27, 25, 23, and 22 kd). in the epidermis, heat-shock protein synthesis peaks at 42 degrees ... | 1990 | 2292612 |
| serological reactions to leptospira species in buffalo (syncerus caffer) from the kruger national park. | four hundred and six serum samples from buffalo (syncerus caffer) were tested for leptospirosis, using the microscopic agglutination test. seven buffaloes (1.7%) reacted positive and 27 (6.6%) inconclusive. reactions against l. tarassovi and l. hardjo were the most prevalent. | 1990 | 2293138 |
| release and absorption characteristics of novel theophylline sustained-release formulations: in vitro-in vivo correlation. | five new experimental sustained-release (sr) formulations of theophylline, t-1, t-1-a, t-2, t-2-a, and t-2-e, in a matrix tablet form with a protein were developed. the in vitro release of theophylline from these novel experimental formulations and two commercial (theotrim and theo-dur) sr formulations, was studied for 2 hr immersed in simulated gastric fluid ts, followed by an additional 10 hr immersed in simulated intestinal fluid ts. like therotrim and theo-dur, theophylline release profiles ... | 1990 | 2293217 |
| aids--an interview with ole morten nygaard who is hiv positive. interview by marie farrell and richard wells. | | 1990 | 2394895 |
| substance p and neurotensin: their roles in the regulation of anterior pituitary function. | | 1986 | 2423025 |
| damage of porcine aortic valve tissue caused by the surfactant sodiumdodecylsulphate. | the hancock t6 treatment uses 1% sodiumdodecylsulphate (sds) to prevent or delay calcification in porcine aortic or pericardial bioprostheses. in the current study fresh and glutaraldehyde fixed porcine aortic cusps were treated in 1% sds. the hydrothermal stability, the histological and the electronmicroscopic appearance of the tissue were assessed and compared before and after treatment. the results suggest that the 1% sds solution destroys the fresh material causing acellularity, extreme frag ... | 1986 | 2424134 |
| identification and characterization of the nmyc gene product in human neuroblastoma cells by monoclonal antibodies with defined specificities. | increased n-myc (now designated nmyc in human gene nomenclature) gene expression has been detected at the transcriptional level in certain types of neoplasms. as yet, the n-myc gene product has not been identified. to detect and characterize the n-myc gene product, we have developed monoclonal antibodies against the putative n-myc gene product made in escherichia coli as a fusion protein. the antibodies that recognize the n-myc-specific regions were selected on the basis of their reactivities to ... | 1986 | 2426708 |
| receptors for substance p on isolated intestinal smooth muscle cells of the guinea pig. | two radioligands, 125i-labeled substance p (125i-sp) and 125i-labeled substance k (125i-sk), were used to characterize the kinetics and stoichiometry of binding of mammalian tachykinins [substance p (sp), substance k (sk), and neuromedin k (nk)] to smooth muscle cells isolated from the longitudinal muscle layer of guinea pig intestine. specific binding of 125i-sp and 125i-sk was rapid, saturable, reversible, and temperature dependent. binding attained 63-70% of steady-state binding within 1 min, ... | 1987 | 2446507 |
| immunosuppressive effect of fk506 on collagen-induced arthritis in rats. | fk506, a new immunosuppressive agent, was given intramuscularly to rats for 12 days, starting on the day of type ii collagen immunization. fk506 in doses of 0.32 mg/kg or more suppressed arthritis and also suppressed humoral and skin test response to type ii collagen. fk506 suppressed arthritis only when given during the afferent limbs of immune response (0-4 days), whereas the drug was only marginally effective when treatment was started during the efferent limbs of immune response (7-11 days). ... | 1988 | 2446810 |
| deficiency in interferon production of peripheral blood leukocytes from patients with non-hodgkin lymphoma. | in search for a rationale for the use of interferons (ifns) in treatment of non-hodgkin lymphoma (nhl), we have investigated the ifn system of 13 patients with low-grade nhl, 15 patients with high-grade nhl, and 20 patients with chronic lymphocytic leukemia or leukemic immunocytoma (cll/ic). production of ifn induced by phytohemagglutinin (pha), concanavalin a (con a), pokeweed mitogen (pwm), corynebacterium parvum, herpes simplex virus (hsv), newcastle disease virus (ndv), and interleukin 2 (il ... | 1988 | 2459268 |
| human neutrophil-mediated lysis of ovarian cancer cells. | because of recent questions concerning the sensitivity of human tumor cells to neutrophil-induced oxidative injury, we studied six freshly obtained human ovarian cancer (oc) specimens. stimulation of neutrophils (pmns) by phorbol myristate acetate (pma) did not result in oc cytolysis during the first nine hours of incubation. however, three of six specimens were significantly lysed by stimulated pmns when assay length was increased to 18 hours. cytotoxicity was mediated by pmn production of reac ... | 1989 | 2546632 |
| syncytium formation by recombinant vaccinia viruses carrying bovine parainfluenza 3 virus envelope protein genes. | the highly syncytium-inducing m strain and the weakly syncytium-inducing sc strain of bovine parainfluenza 3 virus differ by a single amino acid substitution in each of the hemagglutinin-neuraminidase (hn) and membrane (m) proteins, while their fusion (f) proteins are identical (t. shioda, s. wakao, s. suzu, and h. shibuta, virology 162:388-396, 1988). we constructed recombinant vaccinia viruses which express separately the m virus hn (vac-mhn), sc virus hn (vac-schn), m virus m (vac-mm), sc vir ... | 1989 | 2547990 |
| characterization of the rela1 mutation and a comparison of rela1 with new rela null alleles in escherichia coli. | the most widely studied "relaxed" mutant of the rela locus, the rela1 allele, is shown here to consist of an is2 insertion between the 85th and 86th codons of the otherwise wild-type rela structural gene, which normally encodes a 743-amino acid (84 kda) protein. the rela protein is a ribosome-dependent atp:gtp (gdp) pyrophosphoryltransferase that is activated during the stringent response to amino acid starvation and thereby occasions the accumulation of guanosine 3',5'-bispyrophosphate (ppgpp). ... | 1989 | 2556396 |
| does adult t-cell leukaemia originate from intestinal mucosa. | | 1989 | 2565495 |
| characterization of rat cerebral cortical beta adrenoceptor subtypes using (-)-[125i]-iodocyanopindolol. | (-)-[125i]-iodocyanopindolol [-)icyp), used to characterize beta adrenoceptors on membrane preparations from rat cerebral cortex, was shown to have affinity for both beta adrenoceptors and serotonin receptors. therefore, 10 microm serotonin was added to the assays to prevent (-)icyp binding to serotonin receptors. under these conditions, (-)icyp binding to the cortical membrane preparation was reversible and saturable, and the association reaction was very slow. the dissociation reaction was als ... | 1989 | 2577008 |
| [platelet-modified ldl: uptake and metabolism in macrophages]. | mouse peritoneal macrophages accumulate large amounts of cholesterol ester when incubated with chemically or biologically modified lipoproteins. incubation of ldl with platelet secretory products for 2 hours at 37 degrees c decreases its protein and cholesterol content. subsequently this modified ldl, named pcm-ldl, enhances in-vitro platelet aggregation. when it is incubated with cultures of macrophages, cholesterol accumulates in the cells and esterification is increased. pcm-ldl is taken up b ... | 1989 | 2583601 |
| [microcirculation in the diaphyses of tubular bones after intramedullary osteosynthesis]. | | 1989 | 2603037 |
| ninety-day inhalation study in rats, comparing smoke from cigarettes that heat tobacco with those that burn tobacco. | eight groups of 30 male and 30 female rats were exposed 1 hr per day, 5 days per week for 13 weeks, to smoke from reference (tobacco burned) or test (tobacco only heated) cigarettes, at nicotine concentrations of 5, 15, or 30 micrograms/liter of air. similar smoke concentrations of wet total particulate matter and carbon monoxide were produced in each of the test/reference comparisons. there was a pronounced depression of minute ventilation of animals in the reference groups, but not in the test ... | 1989 | 2612779 |
| repair of experimental arteriotomy in rabbit aorta using a new resorbable elastin-fibrin biomaterial. | a new artificial connective matrix which results from two reactions of fibrinogen and fibronectin on elastin was used to obturate a slit made in the abdominal aorta of rabbit. the so-called elastin-fibrin biomaterial behaved as a scaffold through which all the different structures were restored to their former condition. at 3 months, the material had disappeared and no thrombus, no inflammation or reject had been detected. | 1989 | 2621215 |
| the rat 78,000 dalton glucose-regulated protein (grp78) as a precursor for the rat steroidogenesis-activator polypeptide (sap): the sap coding sequence is homologous with the terminal end of grp78. | based on the striking sequence identity between the amino acid sequence of rat steroidogenesis-activator polypeptide (sap) and the carboxyl terminus of the 78,000 dalton glucose-regulated protein (grp78), the precursor-product relationship between grp78 and sap was investigated in leydig cells. immunoblot analysis with peptide antibodies specific for grp78 and sap showed that the putative sap precursor is also immunoreactive with the anti-grp78 antibody. genomic blot hybridizations further revea ... | 1989 | 2628731 |
| [angiographic course of coronary atherosclerosis in angina pectoris. its relation to 2 years' treatment with propranolol, nifedipine and nitrates]. | calcium antagonists and beta-blockers may retard or inhibit atherogenesis. we tested whether nifedipine or propranolol may retard or induce regression of coronary atherosclerosis in man. in selected population of 113 patients with effort angina and proven coronary artery disease, the coronary cineangiographic pattern after 2 year therapy with nifedipine (group 1, 39 patients), propranolol (group 2, 36 patients), or isosorbide dinitrate (control group, 38 patients) was compared to the pre-treatme ... | 1989 | 2631989 |
| [angiographic course of coronary atherosclerosis in angina pectoris. its relation to 2 years' treatment with propranolol, nifedipine and nitrates]. | calcium antagonists and beta-blockers may retard or inhibit atherogenesis. we tested whether nifedipine or propranolol may retard or induce regression of coronary atherosclerosis in man. in selected population of 113 patients with effort angina and proven coronary artery disease, the coronary cineangiographic pattern after 2 year therapy with nifedipine (group 1, 39 patients), propranolol (group 2, 36 patients), or isosorbide dinitrate (control group, 38 patients) was compared to the pre-treatme ... | 1989 | 2631989 |
| nasal obstruction and human communication. | nasal obstruction may cause a variety of communication disorders, particularly in children. the effects of nasal obstruction on hearing, speech, language, and voice are examined. methods for assessing the effects of nasal obstruction are delineated, and recommendations for therapeutic interventions are described. | 1989 | 2664661 |
| studies on interactions of endotoxin with factors of the contact system of plasma. | | 1989 | 2675058 |
| in vitro evaluation of the determinants of bactericidal activity of ampicillin dosing regimens against escherichia coli. | an in vitro flow model was used to examine the influence of peak concentration (cmax), the area under the antibiotic concentration-time curve (auc), the magnitude of auc above the mic, and the aggregate time the antibiotic concentration exceeds the mic (tmic) on the bactericidal effect of ampicillin against escherichia coli atcc 12407. bacteria in the log phase were exposed to therapeutically realistic drug regimens. ampicillin concentration and bacterial density (cfu per milliliter) were measur ... | 1989 | 2675754 |
| in vitro evaluation of the determinants of bactericidal activity of ampicillin dosing regimens against escherichia coli. | an in vitro flow model was used to examine the influence of peak concentration (cmax), the area under the antibiotic concentration-time curve (auc), the magnitude of auc above the mic, and the aggregate time the antibiotic concentration exceeds the mic (tmic) on the bactericidal effect of ampicillin against escherichia coli atcc 12407. bacteria in the log phase were exposed to therapeutically realistic drug regimens. ampicillin concentration and bacterial density (cfu per milliliter) were measur ... | 1989 | 2675754 |
| tolerance of mefloquine alone and in combination with sulfadoxine-pyrimethamine in the prophylaxis of malaria. | a randomized double blind study was performed to evaluate the tolerance and the acceptance of mefloquine alone (lariam) compared to a combined drug regimen consisting of mefloquine, sulfadoxine and pyrimethamine (msp; fansimef) in the prophylaxis of malaria. 175 europeans travelling to different malaria endemic areas received either mefloquine alone (250 mg/week) or its combination with sulfadoxine (500 mg/week) plus pyrimethamine (25 mg/week). one person taking mefloquine and two taking msp dis ... | 1989 | 2694482 |
| current management of salivary gland tumors. part 2. | the authors base their treatment of salivary gland malignancies on the size of the primary and the histopathologic diagnosis. group 1 includes smaller tumors in the t1 and t2 classification with cell types that are associated with slow growth. a parotidectomy is usually sufficient therapy for tumors in this group. group 2 contains t1 and 2 tumors with more aggressive behavior. total parotidectomy is indicated here, with postoperative radiotherapy. t3 tumors and patients with nodal metastasis or ... | 1989 | 2701414 |
| in vivo dna cross-linking by cyclophosphamide: comparison of human chronic lymphatic leukemia cells with mouse l1210 leukemia and normal bone marrow cells. | alkaline elution was done on a variety of cells following cyclophosphamide (cy) treatment in vivo. cells used were l1210 leukemia, normal mouse bone marrow, and peripheral blood cells obtained from a patient with chronic lymphatic leukemia (cll). endpoints used were determination of single strand breaks, dna-dna interstrand and dna-protein cross-links. after treatment of mice with cy (4 mg/mouse), low levels of single strand breaks were observed in both l1210 leukemia and cdf1 normal bone marrow ... | 1989 | 2731167 |
| human gastric lipase. the n-terminal tetrapeptide is essential for lipid binding and lipase activity. | human gastric lipase subjected to limited tryptic proteolysis lost its ability to hydrolyze emulsified long-chain triacylglycerol. activity against a water-soluble substrate was however retained, indicating that proteolysis did not affect the active site. sequence analysis revealed that trypsin specifically cleaved the linkage between lysine-4 and leucine-5. this cleavage rendered the enzyme unable to bind to emulsified triacylglycerol particles, e.g. human milk fat globules. we suggest that the ... | 1989 | 2753032 |
| san people (bushmen) from the central kalahari desert found free of antibodies to hiv-1 and hiv-2. | | 1989 | 2754614 |
| adaptation of contractile proteins in human heart and skeletal muscles. | sarcomeric muscles including the myocardium respond to increased workload with adaptation by differential gene expression and without cell division. this is characteristic for postmitotic tissues. we showed that a short period of high intensity endurance training induces a shift from fast myosin heavy (hc) and light (lc) chain isoforms towards the slow variety within histochemically typed fibres of human vastus lateralis muscle. in an intermediary state of transition both varieties of isoforms a ... | 1989 | 2757604 |
| computation of the effective body mass for metabolic studies of lean and obese rats. | the whole-animal metabolic rates of lean and obese rats cannot be directly compared because obese rats have much greater body mass than lean rats. expressing the results as metabolic rate per unit of body mass is also not satisfactory because lean and obese rats of the same size have different body compositions. to make possible comparisons between the two types of rats, metabolic rate must be expressed per unit of effective body mass. effective body mass for lean and obese zucker rats can be ca ... | 1989 | 2761413 |
| biomagnetic functional localization of a peripheral nerve in man. | the first detection of the magnetic field of a stimulated peripheral nerve in man is presented. the measurement was performed noninvasively and in vivo on a healthy subject. the spatio-temporal field distribution is utilized to calculate the location of bioelectric activity on the basis of the equivalent current dipole model. the localization of the active nerve tissue is confirmed by a computer tomography image of the upper arm cross-section. furthermore, a calculation of the total current dist ... | 1989 | 2765652 |
| the mrna for alpha 1-acid glycoprotein is deadenylated during stimulation by dexamethasone. | | 1989 | 2780624 |
| seasonal abundance and parity of culicoides biting midges associated with livestock at roma, lesotho (diptera: ceratopogonidae). | a light-trap survey was undertaken of the species composition, seasonal abundance and parity of culicoides at roma, lesotho, to establish whether the likely vectors for bluetongue and african horse sickness occur in this area as well as the chance of transmission. a total of 34 catches was made between 21 september 1985 and 24 september 1986; 32,819 culicoides were caught belonging to 19 species. culicoides numbers rapidly built up from december to a peak in february which implies that this may ... | 1989 | 2812701 |
| a caring perspective on rehabilitation after myocardial infarction. a theoretical framework and a suggestion for a rehabilitation programme. | several rehabilitation models after myocardial infarction (mi) have been developed during recent years, usually with a biomedical perspective. the mi client is a biopsychosocial human being and therefore a multifactorial approach built on holistic care may offer an efficient rehabilitation process. this includes increased social support and decreased life stress for the mi client in order to obtain an adaptive coping capacity for a life style change. health care professionals are required with s ... | 1989 | 2814082 |
| a caring perspective on rehabilitation after myocardial infarction. a theoretical framework and a suggestion for a rehabilitation programme. | several rehabilitation models after myocardial infarction (mi) have been developed during recent years, usually with a biomedical perspective. the mi client is a biopsychosocial human being and therefore a multifactorial approach built on holistic care may offer an efficient rehabilitation process. this includes increased social support and decreased life stress for the mi client in order to obtain an adaptive coping capacity for a life style change. health care professionals are required with s ... | 1989 | 2814082 |
| use of nude (athymic) mice for the study of hypertrophic scars and keloids: vascular continuity between mouse and implants. | hypertrophic scars and keloids appear to be unique to humans since animals are not known to form these lesions. therefore, in an effort to develop an experimental model for their study, implants of these human lesions were made in nude (athymic) mice (nu/nu) in suprascapular subcutaneous pockets. the implants were recovered from 2 to 246 days. by histological and fine structural parameters all implants remained viable and their morphological character was maintained. selected mice were injected ... | 1989 | 2817436 |
| papillary syringadenoma of the lower lip: report of a case. | a case of papillary syringadenoma that was located on the right side of the vermilion border of the lower lip in a 24-year-old man is described. the neoplastic epithelium showed two types of carbohydrate with variable topographical distribution and histochemical reaction. the cystic tumor areas showed subepithelial accumulations of mucopolysaccharides, probably related to topical collagen degradation. | 1987 | 2821211 |
| a molecular strategy for the study of bacterial invasion. | bacterial populations are often clonal, and even within a bacterial species, the frequency of gene exchange and recombination is quite low. consequently, mobile genetic elements--plasmids, bacteriophages, and transposons--have been the central factors in the evolution of pathogenic traits. one central feature of pathogenicity, the capacity to enter epithelial cells, is encoded by the bacterial chromosome of yersinia pseudotuberculosis but by plasmid genes in enteroinvasive escherichia coli. a si ... | 1987 | 2825322 |
| saccharomyces cerevisiae mutant with a partial defect in the synthesis of cdp-diacylglycerol and altered regulation of phospholipid biosynthesis. | a saccharomyces cerevisiae mutant (cdg1 mutation) was isolated on the basis of an inositol excretion phenotype and exhibited pleiotropic deficiencies in phospholipid biosynthesis. genetic analysis of the mutant confirmed that the cdg1 mutation represents a new genetic locus and that a defect in a single gene was responsible for the cdg1 phenotype. cdp-diacylglycerol synthase activity in mutant haploid cells was 25% of the wild-type derepressed level. biochemical and immunoblot analyses revealed ... | 1988 | 2832385 |
| a dot-immunobinding assay for infectious bronchitis virus. | common whatman filter paper grade 1 and nitrocellulose membrane were compared for their sensitivity in a dot-immunobinding assay for detection of serum antibody titers to arkansas avian infectious bronchitis virus (aibv). for a blue to purple color detection, serum antibodies were bound to aibv antigen adsorbed on the filter-paper discs or nitrocellulose membrane. rabbit anti-chicken igg horseradish-peroxidase (hrp) conjugate and hydrogen peroxide with 4-chloro-1-naphthol (hrp-color development ... | 1988 | 2838014 |
| mk-801, a proposed noncompetitive antagonist of excitatory amino acid neurotransmission, produces phencyclidine-like behavioral effects in pigeons, rats and rhesus monkeys. | the behavioral effects of mk-801 [(+)-5-methyl-10,11-dihydroxy-5h-dibenzo(a,d)cyclohepten-5,10-imin e], a proposed noncompetitive n-methyl-d-aspartate (nmda) antagonist, were compared to those of phencyclidine (pcp). in pigeons, mk-801 produced pcp-like catalepsy (i.e., loss of righting without eye closure and without muscle relaxation) and pcp-like discriminative stimulus effects. in rats, mk-801 produced pcp-like behavior (i.e., locomotion, sniffing, swaying and falling). in rhesus monkeys, li ... | 1988 | 2838610 |
| an acyclovir-resistant strain of herpes simplex virus type 2 which is highly virulent for mice. | herpes simplex virus type 2 (hsv-2), strain ys-4 c-1, isolated by plaque cloning from a clinical isolate was found to be resistant to acyclovir (acv; acycloguanosine) in vitro. it was sensitive to phosphonoacetic acid and 9-beta-d-arabinofuranosyladenine. thymidine kinase (tk) activity of ys-4 c-1 was less than 1% of that of other strains from the same clinical source. however, thymidine plaque autoradiography showed that ys-4 c-1 was not completely deficient in tk activity. ys-4 c-1 showed high ... | 1988 | 2845890 |