| spectroscopic evidence for an all-ferrous [4fe-4s]0 cluster in the superreduced activator of 2-hydroxyglutaryl-coa dehydratase from acidaminococcus fermentans. | the key enzyme of the fermentation of glutamate by acidaminococcus fermentans, 2-hydroxyglutaryl-coenzyme a dehydratase, catalyzes the reversible syn-elimination of water from (r)-2-hydroxyglutaryl-coenzyme a, resulting in (e)-glutaconylcoenzyme a. the dehydratase system consists of two oxygen-sensitive protein components, the activator (hgdc) and the actual dehydratase (hgdab). previous biochemical and spectroscopic studies revealed that the reduced [4fe-4s]+ cluster containing activator transf ... | 2008 | 18274792 |
| an asymmetric model for na+-translocating glutaconyl-coa decarboxylases. | glutaconyl-coa decarboxylase (gcd) couples the biotin-dependent decarboxylation of glutaconyl-coa with the generation of an electrochemical na(+) gradient. sequencing of the genes encoding all subunits of the clostridium symbiosum decarboxylase membrane complex revealed that it comprises two distinct biotin carrier subunits, gcdc(1) and gcdc(2), which differ in the length of a central alanine- and proline-rich linker domain. co-crystallization of the decarboxylase subunit gcda with the substrate ... | 2009 | 19654317 |
| complete genome sequence of acidaminococcus fermentans type strain (vr4). | acidaminococcus fermentans (rogosa 1969) is the type species of the genus acidaminococcus, and is of phylogenetic interest because of its isolated placement in a genomically little characterized region of the firmicutes. a. fermentans is known for its habitation of the gastrointestinal tract and its ability to oxidize trans-aconitate. its anaerobic fermentation of glutamate has been intensively studied and will now be complemented by the genomic basis. the strain described in this report is a no ... | 2010 | 21304687 |
| substrate specificity of 2-hydroxyglutaryl-coa dehydratase from clostridium symbiosum: toward a bio-based production of adipic acid. | expression of six genes from two glutamate fermenting clostridia converted escherichia coli into a producer of glutaconate from 2-oxoglutarate of the general metabolism (djurdjevic, i. et al. 2010, appl. environ. microbiol.77, 320-322). the present work examines whether this pathway can also be used to reduce 2-oxoadipate to (r)-2-hydroxyadipic acid and dehydrate its coa thioester to 2-hexenedioic acid, an unsaturated precursor of the biotechnologically valuable adipic acid (hexanedioic acid). 2 ... | 2011 | 21434666 |
| long-term outpatient vasodilator therapy of congestive heart failure. consideration of agents at rest and during exercise. | increased left ventricular filling pressure and reduced cardiac output are two major hemodynamic deficits in pump failure. in patients with chronic heart failure, consequences of these hemodynamic deficits and diminished cardiac reserve are manifested initially during stress and eventually at rest. the purpose of therapeutic interventions include reduction of ventricular filling pressure increase in cardiac output and improvement in cardiac reserve. to achieve these goals, the hemodynamic effect ... | 2001 | 99031 |
| [problem of overlapping of collagen diseases with other related diseases of autoimmune diseases--chronic thyroiditis]. | | 2002 | 135106 |
| handlight retroillumination in hotv visual acuity testing. | | 2013 | 4062629 |
| letter: bromide intoxication. | | 2002 | 126712 |
| [data on the tumor-inducing effect of n-methyl-n-nitrosourea]. | | 2013 | 3861333 |
| [data on the tumor-inducing effect of n-methyl-n-nitrosourea]. | | 2013 | 3861333 |
| clomipramine and clonazepam in cataplexy. | | 2000 | 91833 |
| performance of cardiac pacemaker pulse generators. | | 2001 | 95319 |
| [study of a further case of bisalbuminemia in brittany]. | a rapidly migrating variant of albumin has been discovered in a 28-year-old breton woman. at least eight relatives also bear the trait. this new variant is described in terms of its electrophoretic mobilities, immunological properties and heat- and storage stability. two other reports of the slow type are presented, but no family study was performed in these cases. it is suggested that bisalbuminaemia could be a relatively common inherited condition in brittany. | 2002 | 134769 |
| seasonal variation of peripheral thyroid hormone levels in major depression. | the seasonal variation in thyroid function tests was examined in 138 patients with major depression. no alterations in thyroxine, free thyroxine index, triiodothyronine, t3 resin uptake and thyrotropin were observed across the four seasons. this applied to both male and female subgroups. these data suggest that seasonality does not account for the wide variability in abnormalities of thyroid function reported in depression. | 2005 | 1774423 |
| treatment of hypertension. | | 2005 | 4250702 |
| effects of tonicity on the resting tension in bullfrog ventricle. | a study of the effects of hyper -and hypotonic media on resting tension were examined in ventricular muscle of the bullfrog was undertaken because only few studies on resting tension from the viewpoint of e-c coupling in the heart muscle are available. | 2005 | 1079889 |
| today's non-orwellian animal farm. | | 2006 | 1538742 |
| nmr relaxation of protein and water protons in diamagnetic hemoglobin solutions. | we have measured t1 and t2 of protein and water protons in hemoglobin solutions using broad-line pulse techniques; selective excitation and detection methods enabled the intrinsic protein and water relaxation rates, as well as the spin-transfer rate between them, to be obtained at 5, 10, and 20 mhz. water and protein t1 data were also obtained at 100 and 200 mhz for hemoglobin in h2o/d2o mixtures by using commercial fourier-transform instruments. the t1 data conform to a simple model of two well ... | 2014 | 4041420 |
| herpes zoster of the stomach. | | 2000 | 91884 |
| [study of adenine mononucleotides of the adductor muscle of the oyster by fractionation on a pei cellulose column]. | adenine mononucleotides of transulucent and white parts in the adductor muscle of japanese oyster are identified and measured after the separation on pei-cellulose column. the studies are performed on oysters kept in aerated sea water aquarium. results obtained for both tissues are compared each other and with the literature data. | 2002 | 134768 |
| [relations between hemoglobin and blood groups in africans]. | | 2014 | 4229327 |
| [experience with the use of electroroentgenography in the diagnosis of endocrine diseases]. | electroentgenographic examination was carried out in 729 patients with various endocrine diseases. it is believed that electroentgenography with consideration to such features as marginal effect, numerous details, volumetric impression, etc. has at its disposal valuable auxillary diagnostic possibilities in the complex examination of the patients. analysis of materials demonstrated electroentgenography to be the method of choice in contrastless x-ray diagnosis of changes in the thyroid and mamma ... | 2004 | 424389 |
| treatment of traumatic perforative fractures of the nasal cavities and paranasal sinus in an american alligator. | | 1999 | 93825 |
| [from our master examination]. | | 2004 | 355976 |
| [fecal incontinence. epidemiology, diagnosis and treatment]. | | 2006 | 1563608 |
| complex partial status epilepticus in a patient with leptomeningeal carcinomatosis. | | 2007 | 2330119 |
| bronchiolitis obliterans organising pneumonia in a patient with ulcerative colitis. | a young woman with ulcerative colitis developed pneumonia, which responded to corticosteroids. histological examination showed this to be bronchiolitis obliterans organising pneumonia. | 2011 | 3194883 |
| [the "optura" system for the injection of thermo-plasticized gutta-percha into root canals]. | | 2013 | 3864811 |
| evaluation of the short child. | | 2014 | 4292473 |
| evaluation of the short child. | | 2014 | 4292473 |
| [abrikossoff's tumor]. | | 2015 | 4365936 |
| antigen-receptor complex stimulation triggers protein kinase c-dependent cd11a/cd18-cytoskeleton association in t lymphocytes. | although it is well accepted that intercellular adhesion involving the cd11a/cd18 (lfa-1) complex is critical in a wide array of t cell-dependent processes, recent demonstrations of an lfa-1 high avidity state, induced by triggering the t cell receptor (tcr) complex, has raised questions about the intracellular signals generated and molecular events leading to effective cell coupling, as well as their orderly sequence. in this study, we assessed the effects of t cell activation on the actin-base ... | 2006 | 1346786 |
| [dose estimation by electron spin resonance (esr) of the tooth enamel of radiologic technologists]. | | 2011 | 3039458 |
| the effects of religious backgrounds on emotional problems. | | 2002 | 126985 |
| [compensatory alveolar inclination in asymmetry of the mandible]. | | 2015 | 4534464 |
| bilateral congenital aplasia of the deep femoral arteries. | a patient with bilateral congenital aplasia of the deep femoral arteries is described. the condition was accompanied by aneurysms of the popliteal arteries. besides the academic interest of such an anatomical rarity, the abnormality is of clinical importance insofar as acute ischemia of the extremity may occur in the presence of superficial femoral artery occlusion. | 2007 | 2223550 |
| bilateral congenital aplasia of the deep femoral arteries. | a patient with bilateral congenital aplasia of the deep femoral arteries is described. the condition was accompanied by aneurysms of the popliteal arteries. besides the academic interest of such an anatomical rarity, the abnormality is of clinical importance insofar as acute ischemia of the extremity may occur in the presence of superficial femoral artery occlusion. | 2007 | 2223550 |
| studies on the mechanism of electron bifurcation catalyzed by electron transferring flavoprotein (etf) and butyryl-coa dehydrogenase (bcd) of acidaminococcus fermentans. | electron bifurcation is a fundamental strategy of energy coupling originally discovered in the q-cycle of many organisms. recently a flavin-based electron bifurcation has been detected in anaerobes, first in clostridia and later in acetogens and methanogens. it enables anaerobic bacteria and archaea to reduce the low-potential [4fe-4s] clusters of ferredoxin, which increases the efficiency of the substrate level and electron transport phosphorylations. here we characterize the bifurcating electr ... | 2014 | 24379410 |
| development of a satisfactory and general continuous assay for aminotransferases by coupling with (r)-2-hydroxyglutarate dehydrogenase. | a continuous general spectrophotometric assay for measuring the activity of aminotransferases has been developed. it is based on the transamination of a keto compound (amino acceptor) and l-glutamate (amino donor), yielding the corresponding amino compound and 2-oxoglutarate. the rate of formation of 2-oxoglutarate is measured in a coupled reaction with overproduced recombinant nicotinamide adenine dinucleotide (nad(+))-dependent (r)-2-hydroxyglutarate dehydrogenase from acidaminococcus fermenta ... | 2012 | 23000002 |
| biological activities of phthalocyanines--vi. photooxidation of l-tryptophan by selectively sulfonated gallium phthalocyanines: singlet oxygen yields and effect of aggregation. | | 2013 | 3602110 |
| single-blind, randomised, parallel group study of the bard biocath catheter and a silicone elastomer coated catheter. | a group of 69 community patients undergoing long-term urethral catheterisation for urinary incontinence took part in this study; 33 patients with a mean age of 70.03 years (+/- 16.6) received the dow corning silastic catheter (16 f 10-ml balloon) and 36 patients with a mean age of 75.61 years (+/- 12.6) received the bard biocath catheter (16 f 10-ml balloon). over a 16-week period catheters were monitored every 2 weeks and changed as necessary. the bard biocath catheter remained in situ for an a ... | 2007 | 1933160 |
| [pavel efimovich zabludovskiĭ]. | | 1974 | 380007 |
| vitamin and mineral usage in adult family practice patients. | | 2013 | 4056668 |
| japan and japanese medicine. | | 2013 | 3618517 |
| teaching with computers. | various methods of using computers in education have been presented. although the interesting file, interactive video, and bibliographic searches stand on their own, a far better approach is to integrate them, so that the radiologist learning new techniques in interventional procedures, for instance, could search the current literature quickly and easily for new articles using the bibliographic search facility and could review interesting cases from the teaching file that pertained to the lesson ... | 2012 | 3515406 |
| the specificity and kinetic mechanism of branched-chain amino acid aminotransferase from escherichia coli studied with a new improved coupled assay procedure and the enzyme's potential for biocatalysis. | branched-chain amino acid aminotransferase (bcat) plays a key role in the biosynthesis of hydrophobic amino acids (such as leucine, isoleucine and valine), and its substrate spectrum has not been fully explored or exploited owing to the inescapable restrictions of previous assays, which were mainly based on following the formation/consumption of the specific branched-chain substrates rather than the common amino group donor/acceptor. in our study, detailed measurements were made using a novel co ... | 2014 | 24206068 |
| reduction of ferredoxin or oxygen by flavin-based electron bifurcation in megasphaera elsdenii. | over 50 years ago, it was reported that, in the anaerobic rumen bacterium megasphaera elsdenii, the reduction of crotonyl-coa to butyryl-coa by nadh involved an electron transferring flavoprotein (etf) as mediator [baldwin rl, milligan lp (1964) biochim biophys acta 92, 421-432]. purification and spectroscopic characterization revealed that this etf contained 2 fad, whereas, in the etfs from aerobic and facultative bacteria, one fad is replaced by amp. recently we detected a similar system in th ... | 2015 | 25903584 |
| reduction of flavodoxin by electron bifurcation and sodium ion-dependent reoxidation by nad+ catalyzed by ferredoxin-nad+ reductase (rnf). | electron-transferring flavoprotein (etf) and butyryl-coa dehydrogenase (bcd) from acidaminococcus fermentans catalyze the endergonic reduction of ferredoxin by nadh, which is also driven by the concomitant reduction of crotonyl-coa by nadh, a process called electron bifurcation. here we show that recombinant flavodoxin from a. fermentans produced in escherichia coli can replace ferredoxin with almost equal efficiency. after complete reduction of the yellow quinone to the blue semiquinone, a seco ... | 2016 | 27048649 |