| [fecal incontinence. epidemiology, diagnosis and treatment]. | | 2006 | 1563608 |
| complex partial status epilepticus in a patient with leptomeningeal carcinomatosis. | | 2007 | 2330119 |
| lethal and sublethal effects of the combination of doxorubicin and the bisdioxopiperazine, (+)-1,2,-bis (3-5-dioxopiperazinyl-1-yl) propane (icrf 187), on murine sarcoma s180 in vitro. | doxorubicin and the bisdioxopiperazine, icrf 187, synergistically inhibit proliferation of murine sarcoma s180 cells in vitro. cell cycle analysis was employed to help discriminate cytokinetic from lethal effects of the drug combination. twenty-four-hour incubation with either agent produced dose-dependent partial g2m arrest. at high doses, icrf 187 produced partial g2m arrest, inhibition of cell division, and continued dna synthesis at a higher ploidy, resulting in a second g2m arrest of an 8n ... | 1987 | 3107574 |
| delayed-amelanotic (dam or smyth) chicken: melanocyte dysfunction in vivo and in vitro. | chickens of the autoimmune delayed-amelanotic (dam or smyth) line develop postnatal feather amelanosis and severe visual defects, both of which are presumed to be due to a dysfunction of melanocytes and a subsequent autoimmune response that eliminates pigment cells. in this report we elucidate further the melanocytic defect. we present a morphologic analysis of the mildly affected erratic (edam) group of smyth chicken whose partial depigmentation and lack of visual impairment resemble human viti ... | 1986 | 3091704 |
| [the "optura" system for the injection of thermo-plasticized gutta-percha into root canals]. | | 2013 | 3864811 |
| japan and japanese medicine. | | 2013 | 3618517 |
| evaluation of the short child. | | 2014 | 4292473 |
| evaluation of the short child. | | 2014 | 4292473 |
| [abrikossoff's tumor]. | | 2015 | 4365936 |
| equilibrium constants of several reactions involved in the fermentation of glutamate. | the equilibrium constants of the reactions catalysed by (s)-citramalate lyase and (r)-2-hydroxyglutarate dehydrogenase were determined using the purified enzymes from clostridium tetanomorphum and acidaminococcus fermentans, respectively. the former constant had to be determined at high ionic strength (i). therefore it was corrected to i = 0.1 m by applying single-ion activity coefficients estimated from literature data. the result (kapp = 4.31 +/- 0.07 m-1; direction of citramalate formation) a ... | 1987 | 2883006 |
| [dose estimation by electron spin resonance (esr) of the tooth enamel of radiologic technologists]. | | 2011 | 3039458 |
| anomalous drainage of the inferior vena cava into a left atrial connection: a case report involving a 41-year-old man. | a 41-year-old man had an anomalous inferior vena cava directly connected to the left atrium demonstrated by contrast echocardiography, magnetic resonance imaging, angiography, and at operation. the anomaly is due to developmental failure of the right sinus venosus valve, septum secundum, and dorsal portion of the atrial septum. | 1989 | 2650882 |
| substrate stereochemistry of the biotin-dependent sodium pump glutaconyl-coa decarboxylase from acidaminococcus fermentans. | the steric course of the decarboxylation of glutaconyl-coa to crotonyl-coa, catalysed by the biotin-dependent sodium pump glutaconyl-coa decarboxylase from acidaminococcus fermentans, was elucidated using the sequence: chiral acetate----citrate----glutamate----glutaconyl-coa----crotonyl-coa ----chiral acetate. since glutaconyl-coa or glutaconate labeled at c-4 was subjected to rapid chemical or enzymatic exchanges, glutamate was fermented to acetate by growing cells of a. fermentans. the analysi ... | 1986 | 2422028 |
| vitamin and mineral usage in adult family practice patients. | | 2013 | 4056668 |
| the sodium pump glutaconyl-coa decarboxylase from acidaminococcus fermentans. specific cleavage by n-alkanols. | glutaconyl-coa decarboxylase from acidaminococcus fermentans was inactivated by incubation with n-alkanols at 37 degrees c. the concentration of the alcohol required for complete inactivation decreased with increasing chain length; e.g. 2 m ethanol was as potent as 2 mm hexanol or 0.5 mm decanol. the data indicate a binding of the alcohol to the enzyme with an energy of about 4 kj/methylene group. sodium ions prevented the inactivation (50% at 30 mm nacl). k+, nh4+, cs+ and mg2+ had no influence ... | 1986 | 2422027 |
| clonal variation of dna repair in a human glioma cell line. | clonal heterogeneity in response to ionizing radiation was found for a human glioma cell line, in859. we have investigated the most sensitive clone, the most resistant clone and the parent line for differences in dna repair fidelity using the method of plasmid reconstitution. significant differences in repair fidelity were found between the two clones, and between the sensitive clone and the parent line. the resistant clone and the parent line showed the greater repair fidelity. a comparison of ... | 1991 | 1924859 |
| regulations for implementing the clinical laboratory improvement amendments of 1988: a summary. | in 1988, congress passed the clinical laboratory improvement amendments (clia), which set standards to improve the quality of clinical laboratory testing in all laboratories in the nation that conduct testing on human specimens for health assessment or for the diagnosis, prevention, or treatment of disease. this report summarizes the final regulations for implementing clia and provides an overview of each section, specifying laboratory standards and requirements. | 1992 | 1538689 |
| single-blind, randomised, parallel group study of the bard biocath catheter and a silicone elastomer coated catheter. | a group of 69 community patients undergoing long-term urethral catheterisation for urinary incontinence took part in this study; 33 patients with a mean age of 70.03 years (+/- 16.6) received the dow corning silastic catheter (16 f 10-ml balloon) and 36 patients with a mean age of 75.61 years (+/- 12.6) received the bard biocath catheter (16 f 10-ml balloon). over a 16-week period catheters were monitored every 2 weeks and changed as necessary. the bard biocath catheter remained in situ for an a ... | 2007 | 1933160 |
| biological activities of phthalocyanines--vi. photooxidation of l-tryptophan by selectively sulfonated gallium phthalocyanines: singlet oxygen yields and effect of aggregation. | | 2013 | 3602110 |
| crystal structure of the acidaminococcus fermentans 2-hydroxyglutaryl-coa dehydratase component a. | acidaminococcus fermentans degrades glutamate via the hydroxyglutarate pathway, which involves the syn-elimination of water from (r)-2-hydroxyglutaryl-coa in a key reaction of the pathway. this anaerobic process is catalyzed by 2-hydroxyglutaryl-coa dehydratase, an enzyme with two components (a and d) that reversibly associate during reaction cycles. component a (compa), a homodimeric protein of 2x27 kda, contains a single, bridging [4fe-4s] cluster and uses the hydrolysis of atp to deliver an e ... | 2001 | 11243821 |
| behavioral effects of dibutyryl cyclic 3'5'amp and biogenic amines in rats. | white male wistar rats were injected intraventricularly with 200 mug of dibutyryl cyclic 3'5'amp (damp. 30 min later part of animals was injected intraventricularly with 10 mug of dopamine (da), 10 mug of 5-hydroxytryptamine (5-ht) or 50 mug of acetylcholine (ach). immediately after injection of the amines the time of walking, convulsions, immobility and number of jumps during 10 min periods of observation was measured. damp induced excitation of animals expressed by convulsions, jumping and sho ... | 1975 | 165657 |
| molecular characterization of phenyllactate dehydratase and its initiator from clostridium sporogenes. | the heterotrimeric phenyllactate dehydratase from clostridium sporogenes, fldabc, catalyses the reversible dehydration of (r)-phenyllactate to (e)-cinnamate in two steps: (i) coa-transfer from the cofactor cinnamoyl-coa to phenyllactate to yield phenyllactyl-coa and the product cinnamate mediated by flda, a (r)-phenyllactate coa-transferase; followed by (ii) dehydration of phenyllactyl-coa to cinnamoyl-coa mediated by heterodimeric fldbc, a phenyllactyl-coa dehydratase. phenyllactate dehydratase ... | 2002 | 11967068 |
| adenosine triphosphate-induced electron transfer in 2-hydroxyglutaryl-coa dehydratase from acidaminococcus fermentans. | 2-hydroxyglutaryl-coa dehydratase from acidaminococcus fermentans catalyzes the chemical difficult elimination of water from (r)-2-hydroxyglutaryl-coa to glutaconyl-coa. the enzyme consists of two oxygen-sensitive protein components, the homodimeric activator (a) with one [4fe-4s]1+/2+ cluster and the heterodimeric dehydratase (d) with one nonreducible [4fe-4s]2+ cluster and reduced riboflavin 5'-monophosphate (fmnh2). for activation, atp, mg2+, and a reduced flavodoxin (16 kda) purified from a. ... | 2002 | 11980491 |
| diet influences the ecology of lactic acid bacteria and escherichia coli along the digestive tract of cattle: neural networks and 16s rdna. | in this manuscript, the authors have sought to gain a better understanding of the interactions between escherichia coli and lactic acid bacteria (lab) isolated from rogossa mrs agar along the digestive tract of grain- and forage-fed cattle. e. coli from cattle receiving a high-grain diet were more numerous (p<0.05) than from the high-forage diet and the highest numbers were in the faeces. isolates on rogossa mrs agar were always greater in the high-grain diet (p<0.05) and contained a significant ... | 2003 | 12576580 |
| a two [4fe-4s]-cluster-containing ferredoxin as an alternative electron donor for 2-hydroxyglutaryl-coa dehydratase from acidaminococcus fermentans. | the key step in the fermentation of glutamate by acidaminococcus fermentans is a reversible syn-elimination of water from ( r)-2-hydroxyglutaryl-coa to ( e)-glutaconyl-coa catalyzed by 2-hydroxyglutaryl-coa dehydratase, a two-component enzyme system. the actual dehydration is mediated by component d, which contains 1.0 [4fe-4s](2+) cluster, 1.0 reduced riboflavin-5'-phosphate and about 0.1 molybdenum (vi) per heterodimer. the enzyme has to be activated by the extremely oxygen-sensitive [4fe-4s]( ... | 2003 | 12610725 |
| crystal structure of the carboxyltransferase subunit of the bacterial sodium ion pump glutaconyl-coenzyme a decarboxylase. | glutaconyl-coa decarboxylase is a biotin-dependent ion pump whereby the free energy of the glutaconyl-coa decarboxylation to crotonyl-coa drives the electrogenic transport of sodium ions from the cytoplasm into the periplasm. here we present the crystal structure of the decarboxylase subunit (gcdalpha) from acidaminococcus fermentans and its complex with glutaconyl-coa. the active sites of the dimeric gcdalpha lie at the two interfaces between the mono mers, whereas the n-terminal domain provide ... | 2003 | 12853465 |
| a 49 kda microtubule cross-linking protein from artemia franciscana is a coenzyme a-transferase. | embryos and larvae of the brine shrimp, artemia franciscana, were shown previously to possess a protein, now termed p49, which cross-links microtubules in vitro. molecular characteristics of p49 were described, but the protein's identity and its role in the cell were not determined. degenerate oligonucleotide primers designed on the basis of peptide sequence obtained by edman degradation during this study were used to generate p49 cdnas by rt-pcr and these were cloned and sequenced. comparison w ... | 2003 | 14653822 |
| dehydration of (r)-2-hydroxyacyl-coa to enoyl-coa in the fermentation of alpha-amino acids by anaerobic bacteria. | several clostridia and fusobacteria ferment alpha-amino acids via (r)-2-hydroxyacyl-coa, which is dehydrated to enoyl-coa by syn-elimination. this reaction is of great mechanistic interest, since the beta-hydrogen, to be eliminated as proton, is not activated (pk 40-50). a mechanism has been proposed, in which one high-energy electron acts as cofactor and transiently reduces the electrophilic thiol ester carbonyl to a nucleophilic ketyl radical anion. the 2-hydroxyacyl-coa dehydratases are two-c ... | 2004 | 15374661 |
| structural basis for stereo-specific catalysis in nad(+)-dependent (r)-2-hydroxyglutarate dehydrogenase from acidaminococcus fermentans. | nad(+)-dependent (r)-2-hydroxyglutarate dehydrogenase (hgdh) catalyses the reduction of 2-oxoglutarate to (r)-2-hydroxyglutarate and belongs to the d-2-hydroxyacid nad(+)-dependent dehydrogenase (d-2-hydroxyacid dehydrogenase) protein family. its crystal structure was determined by phase combination to 1.98 a resolution. structure-function relationships obtained by the comparison of hgdh with other members of the d-2-hydroxyacid dehydrogenase family give a chemically satisfying view of the subst ... | 2005 | 15634349 |
| dual mechanisms of tricarboxylate transport and catabolism by acidaminococcus fermentans. | acidaminococcus fermentans utilized citrate or the citrate analog aconitate as an energy source for growth, and these tricarboxylates were used simultaneously. citrate utilization and uptake showed biphasic kinetics. high-affinity citrate uptake had a k(t) of 40 mum, but the v(max) was only 25 nmol/mg of protein per min. low-affinity citrate utilization had a 10-fold higher v(max), but the k(s) was greater than 1.0 mm. aconitate was a competitive inhibitor (k(i) = 34mum) of high-affinity citrate ... | 1994 | 16349331 |
| acidaminococcus intestini sp. nov., isolated from human clinical samples. | eleven strains of a hitherto unknown, gram-negative, anaerobic coccus were recovered from various human clinical samples of patients hospitalized in two geographically distant french hospitals. these strains displayed the morphology and growth characteristics of those related to the genus acidaminococcus. the clinical isolates shared at least 99.9 and 99.7 % of their nucleotide positions in the 16s and 23s rrna gene sequences, respectively. they displayed 95.6 and 88.9 % 16s and 23s rrna gene se ... | 2007 | 17911303 |
| an asymmetric model for na+-translocating glutaconyl-coa decarboxylases. | glutaconyl-coa decarboxylase (gcd) couples the biotin-dependent decarboxylation of glutaconyl-coa with the generation of an electrochemical na(+) gradient. sequencing of the genes encoding all subunits of the clostridium symbiosum decarboxylase membrane complex revealed that it comprises two distinct biotin carrier subunits, gcdc(1) and gcdc(2), which differ in the length of a central alanine- and proline-rich linker domain. co-crystallization of the decarboxylase subunit gcda with the substrate ... | 2009 | 19654317 |
| complete genome sequence of acidaminococcus fermentans type strain (vr4). | acidaminococcus fermentans (rogosa 1969) is the type species of the genus acidaminococcus, and is of phylogenetic interest because of its isolated placement in a genomically little characterized region of the firmicutes. a. fermentans is known for its habitation of the gastrointestinal tract and its ability to oxidize trans-aconitate. its anaerobic fermentation of glutamate has been intensively studied and will now be complemented by the genomic basis. the strain described in this report is a no ... | 2010 | 21304687 |
| substrate specificity of 2-hydroxyglutaryl-coa dehydratase from clostridium symbiosum: toward a bio-based production of adipic acid. | expression of six genes from two glutamate fermenting clostridia converted escherichia coli into a producer of glutaconate from 2-oxoglutarate of the general metabolism (djurdjevic, i. et al. 2010, appl. environ. microbiol.77, 320-322). the present work examines whether this pathway can also be used to reduce 2-oxoadipate to (r)-2-hydroxyadipic acid and dehydrate its coa thioester to 2-hexenedioic acid, an unsaturated precursor of the biotechnologically valuable adipic acid (hexanedioic acid). 2 ... | 2011 | 21434666 |
| [study of a further case of bisalbuminemia in brittany]. | a rapidly migrating variant of albumin has been discovered in a 28-year-old breton woman. at least eight relatives also bear the trait. this new variant is described in terms of its electrophoretic mobilities, immunological properties and heat- and storage stability. two other reports of the slow type are presented, but no family study was performed in these cases. it is suggested that bisalbuminaemia could be a relatively common inherited condition in brittany. | 2002 | 134769 |
| the specificity and kinetic mechanism of branched-chain amino acid aminotransferase from escherichia coli studied with a new improved coupled assay procedure and the enzyme's potential for biocatalysis. | branched-chain amino acid aminotransferase (bcat) plays a key role in the biosynthesis of hydrophobic amino acids (such as leucine, isoleucine and valine), and its substrate spectrum has not been fully explored or exploited owing to the inescapable restrictions of previous assays, which were mainly based on following the formation/consumption of the specific branched-chain substrates rather than the common amino group donor/acceptor. in our study, detailed measurements were made using a novel co ... | 2014 | 24206068 |
| bronchiolitis obliterans organising pneumonia in a patient with ulcerative colitis. | a young woman with ulcerative colitis developed pneumonia, which responded to corticosteroids. histological examination showed this to be bronchiolitis obliterans organising pneumonia. | 2011 | 3194883 |
| teaching with computers. | various methods of using computers in education have been presented. although the interesting file, interactive video, and bibliographic searches stand on their own, a far better approach is to integrate them, so that the radiologist learning new techniques in interventional procedures, for instance, could search the current literature quickly and easily for new articles using the bibliographic search facility and could review interesting cases from the teaching file that pertained to the lesson ... | 2012 | 3515406 |
| antigen-receptor complex stimulation triggers protein kinase c-dependent cd11a/cd18-cytoskeleton association in t lymphocytes. | although it is well accepted that intercellular adhesion involving the cd11a/cd18 (lfa-1) complex is critical in a wide array of t cell-dependent processes, recent demonstrations of an lfa-1 high avidity state, induced by triggering the t cell receptor (tcr) complex, has raised questions about the intracellular signals generated and molecular events leading to effective cell coupling, as well as their orderly sequence. in this study, we assessed the effects of t cell activation on the actin-base ... | 2006 | 1346786 |
| the effects of religious backgrounds on emotional problems. | | 2002 | 126985 |
| [compensatory alveolar inclination in asymmetry of the mandible]. | | 2015 | 4534464 |
| bilateral congenital aplasia of the deep femoral arteries. | a patient with bilateral congenital aplasia of the deep femoral arteries is described. the condition was accompanied by aneurysms of the popliteal arteries. besides the academic interest of such an anatomical rarity, the abnormality is of clinical importance insofar as acute ischemia of the extremity may occur in the presence of superficial femoral artery occlusion. | 2007 | 2223550 |
| bilateral congenital aplasia of the deep femoral arteries. | a patient with bilateral congenital aplasia of the deep femoral arteries is described. the condition was accompanied by aneurysms of the popliteal arteries. besides the academic interest of such an anatomical rarity, the abnormality is of clinical importance insofar as acute ischemia of the extremity may occur in the presence of superficial femoral artery occlusion. | 2007 | 2223550 |
| [pavel efimovich zabludovskiĭ]. | | 1974 | 380007 |
| studies on the mechanism of electron bifurcation catalyzed by electron transferring flavoprotein (etf) and butyryl-coa dehydrogenase (bcd) of acidaminococcus fermentans. | electron bifurcation is a fundamental strategy of energy coupling originally discovered in the q-cycle of many organisms. recently a flavin-based electron bifurcation has been detected in anaerobes, first in clostridia and later in acetogens and methanogens. it enables anaerobic bacteria and archaea to reduce the low-potential [4fe-4s] clusters of ferredoxin, which increases the efficiency of the substrate level and electron transport phosphorylations. here we characterize the bifurcating electr ... | 2014 | 24379410 |
| reduction of flavodoxin by electron bifurcation and sodium ion-dependent reoxidation by nad+ catalyzed by ferredoxin-nad+ reductase (rnf). | electron-transferring flavoprotein (etf) and butyryl-coa dehydrogenase (bcd) from acidaminococcus fermentans catalyze the endergonic reduction of ferredoxin by nadh, which is also driven by the concomitant reduction of crotonyl-coa by nadh, a process called electron bifurcation. here we show that recombinant flavodoxin from a. fermentans produced in escherichia coli can replace ferredoxin with almost equal efficiency. after complete reduction of the yellow quinone to the blue semiquinone, a seco ... | 2016 | 27048649 |
| reduction of ferredoxin or oxygen by flavin-based electron bifurcation in megasphaera elsdenii. | over 50 years ago, it was reported that, in the anaerobic rumen bacterium megasphaera elsdenii, the reduction of crotonyl-coa to butyryl-coa by nadh involved an electron transferring flavoprotein (etf) as mediator [baldwin rl, milligan lp (1964) biochim biophys acta 92, 421-432]. purification and spectroscopic characterization revealed that this etf contained 2 fad, whereas, in the etfs from aerobic and facultative bacteria, one fad is replaced by amp. recently we detected a similar system in th ... | 2015 | 25903584 |
| development of a satisfactory and general continuous assay for aminotransferases by coupling with (r)-2-hydroxyglutarate dehydrogenase. | a continuous general spectrophotometric assay for measuring the activity of aminotransferases has been developed. it is based on the transamination of a keto compound (amino acceptor) and l-glutamate (amino donor), yielding the corresponding amino compound and 2-oxoglutarate. the rate of formation of 2-oxoglutarate is measured in a coupled reaction with overproduced recombinant nicotinamide adenine dinucleotide (nad(+))-dependent (r)-2-hydroxyglutarate dehydrogenase from acidaminococcus fermenta ... | 2012 | 23000002 |