| localization of the kras2 oncogene in the domestic rabbit (oryctolagus cuniculus). | the kras2 gene was localized in the rabbit by chromosomal in situ hybridization, using a 3h-labeled human cdna probe. there were 201 silver grains on 144 metaphase spreads; 12.9% of the grains resided on chromosome 16, and 54% of these grains were located close to the centromere at 16p11----q11. statistical analysis indicated that labeling at this region represents a significant deviation from a random distribution and thus provided evidence for the assignment of kras2 to 16p11----q11. in additi ... | 1988 | 3073912 |
| estradiol-binding proteins from mycelial and yeast-form cultures of paracoccidioides brasiliensis. | paracoccidioides brasiliensis, the etiologic agent of paracoccidioidomycosis, causes disease much more frequently in men than it does in women, suggesting that the hormonal milieu of the host might influence p. brasiliensis pathogenicity. we recently demonstrated that cytosol from yeast cultures of p. brasiliensis contains a high-affinity, low-capacity, tritiated 17 beta-estradiol [( 3h]estradiol)-binding protein. estradiol and, to a lesser degree, diethylstilbestrol (des), inhibited the transfo ... | 1986 | 3079729 |
| the ability of single sex infections of schistosoma japonicum to induce resistance to reinfection in mice. | in four experiments, mice harbouring an average 50, 76 or over 200 schistosoma japonicum female worms were not resistant when challenged six to eight weeks after infection. the female worms from these single sex infections were stunted and immature (average length 4.6 mm) and induced no overt pathology in the host. male worm burdens of 60, 135 or 140 also induced little or no resistance to challenge in the host. the males from these single sex infections were fully grown for their age (average l ... | 1986 | 3091678 |
| fermentation of xylans by butyrivibrio fibrisolvens and other ruminal bacteria. | the ability of butyrivibrio fibrisolvens and other ruminal bacteria (6 species, 18 strains) to ferment a crude xylan from wheat straw or to ferment xylans from larchwood or oat spelts was studied. liquid cultures were monitored for carbohydrate utilization, cell growth (protein), and fermentation acid production. b. fibrisolvens 49, h17c, actf2, and d1 grew almost as well on one or more of the xylans as they did on cellobiose-maltose. b. fibrisolvens 12, r28, a38, x10c34, ard22a, and x6c61 exhib ... | 1987 | 3124741 |
| some effects of arsenic on the rumen microflora; an in vitro study. | the rate of fermentation of the rumen microflora was inhibited almost 30% by 5 micrograms/ml of arsenic added in the form of arsenite, although 304 micrograms/ml was required to cause 50% inhibition. arsenate was less inhibitory. the rate of fermentation of a separated bacterial fraction was inhibited 37% by 1 microgram of arsenite per millilitre, whereas 100 micrograms/ml had little effect on the fermentation of a separated protozoal fraction. similar results were obtained for arsenate. both fr ... | 1978 | 754875 |
| cellobiose uptake by the cellulolytic ruminal anaerobe fibrobacter (bacteroides) succinogenes. | cellobiose transport by the cellulolytic ruminal anaerobe fibrobacter (bacteroides) succinogenes was measured using randomly tritiated cellobiose. when assayed at the same concentration (1 mm), total cellobiose uptake was one-fourth to one-third that of total glucose uptake. the abilities of f. succinogenes to transport cellobiose or glucose were not affected by the sugar on which the cells were grown. aspects of the simultaneous transport of [14c(u)]glucose and [3h(g)]cellobiose, the failure of ... | 1991 | 2059920 |
| molecular cloning, expression, and characterization of endoglucanase genes from fibrobacter succinogenes ar1. | a cosmid gene library was constructed in escherichia coli from genomic dna isolated from the ruminal anaerobe fibrobacter succinogenes ar1. clones were screened on carboxymethyl cellulose, and 8 colonies that produced large clearing zones and 25 colonies that produced small clearing zones were identified. southern blot hybridization revealed the existence of at least three separate genes encoding cellulase activity. prc093, which is representative of cosmid clones that produce large clearing zon ... | 1991 | 2014986 |
| non-immune igg f(ab')2 binding to group c and g streptococci is mediated by structures on gamma chains. | the present investigation was designed to determine whether the heavy or the light immunoglobulin chain is involved in the non-immune binding of igg f(ab')2 fragments to specific surface receptors on human group c and g streptococci. purified human polyclonal igg was mildly reduced with dithiothreitol and alkylated with iodoacetamide. light (l) and heavy (h) chains were separated. intact igg and purified l and h chains of polyclonal immunoglobulin g were tested in an inhibition assay for non-imm ... | 1985 | 3156400 |
| development of the rumen digestive functions in lambs placed in a sterile isolator a few days after birth. | the development of the rumen digestive functions was studied in lambs placed in sterile isolators at 1, 4, 8 or 9 days of age to define the role of the bacterial species that colonize the rumen just after birth. the values of the main rumen digestive parameters (ph, concentrations of volatile fatty acid, ammonia, lactic acid) in these lambs were close to those observed in conventional controls. likewise, the digestive utilisation of the dry matter and starch was comparable in isolated and contro ... | 1991 | 1768310 |
| effects of plasma ochratoxin a and luminal ph on the jejunal absorption of ochratoxin a in rats. | the effects of iv injection of ochratoxin a (oa) on its absorption from the jejunum in rats were studied in vivo to demonstrate the jejunal absorption of oa in relation to the oa level in blood plasma. in addition the effects of the ph of the medium on oa uptake by the everted jejunum of the rat were studied in vitro to assess the contribution made by transfer of the non-ionized form of oa to the jejunal absorption of the toxin. the in vivo study showed that oa was absorbed from the jejunum even ... | 1988 | 3209137 |
| fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology. | fluorescent-dye-conjugated oligonucleotides were used to classify 14 fibrobacter strains by fluorescence microscopy. on the basis of partial 16s rrna sequences of six fibrobacter strains, four hybridization probes were designed to discriminate between the species fibrobacter succinogenes and fibrobacter intestinalis and to identify f. succinogenes subsp. succinogenes. after in situ hybridization to whole cells of the six sequenced strains, epifluorescence microscopy confirmed probe specificity. ... | 1990 | 1688842 |
| intracellular processing of measles virus fusion protein. | intracellular processing of measles virus fusion (f) protein was studied by radiolabeling and immunoprecipitation with a monoclonal antibody against f protein. the cleavage of f protein into f1 and f2 subunits was complete after 5 hours of chase during which the growth of oligosaccharide chains on the f2 domain of f protein continued. the addition of terminal sialic acid conferred a strong negative charge on the f2 subunit. f protein expressed on the cell surface was removed by a fungal semi-alk ... | 1988 | 3277595 |
| futile cycling of glycogen in fibrobacter succinogenes as shown by in situ 1h-nmr and 13c-nmr investigation. | glycogen was synthesized during all the growth phases in the rumen anaerobic cellulolytic bacterium fibrobacter succinogenes. glycogen synthesis and degradation were monitored using in situ 13c and 1h-nmr spectroscopy in resting cells of f. succinogenes. the cells were incubated at 37 degrees c under anaerobic conditions with [1-13c]glucose and [2-13c]glucose. 1h-nmr spectra were used to quantify enrichment by 13c of metabolism products. glucose was utilized for energy requirements of the bacter ... | 1992 | 1628646 |
| alpha-adrenoceptor responsiveness in the aged rat. | the effects of ageing on vascular alpha 1- and alpha 2-adrenoceptors were examined using anaesthetised and pithed young (3-7 months) and old (21-24 months) sprague-dawley rats. in pithed animals, the pressor and cardioinhibitory effects of the alpha 2-adrenoceptor agonist xylazine were significantly reduced in old animals (8- and 6-fold shift), but the pressor effects of the alpha 1-adrenoceptor agonist amidephrine were not significantly altered by ageing. in anaesthetised rats, the pressor resp ... | 1986 | 3019712 |
| activity of fiber-degrading microorganisms in the pig large intestine. | the large intestine is comparable to the rumen fermentation in many aspects; however, it is understood less well. fiber in the form of cellulose and hemicellulose is one of the major substrates fermented in the large intestine. various studies suggest that the pig can utilize fiber for growth, and up to 30% of its maintenance energy may be derived from volatile fatty acids produced in the large intestine. the total number of microorganisms in the pig large intestine do not change when a high fib ... | 1987 | 3040656 |
| [nosocomial infections of the urinary tract in urology patients]. | researches effected in different nations have revealed that 5-12% of all hospitalized patients was affected with a nosocomial infection and that almost 1/3 of these were urinary tract infections. the hospitalized urological patients have a considerable proneness to contract a nosocomial urinary tract infection, because they are subjected to transurethral or suprapubic bladder catheterisms, to transurethral surgery and to the endoscopies, that are promoting maneuvers the urinary tract infections. ... | 1988 | 3340313 |
| purification and properties of a 1,3-1,4-beta-d-glucanase (lichenase, 1,3-1,4-beta-d-glucan 4-glucanohydrolase, ec 3.2.1.73) from bacteroides succinogenes cloned in escherichia coli. | a 1,3-1,4-beta-d-glucanase (lichenase, 1,3-1,4-beta-d-glucan 4-glucanohydrolase, ec 3.2.1.73) from bacteroides succinogenes cloned in escherichia coli was purified 600-fold by chromatography on q-sepharose and hydroxyapatite. the cloned enzyme hydrolysed lichenin and oat beta-d-glucan but not starch, cm(carboxymethyl)-cellulose, cm-pachyman, laminarin or xylan. the enzyme had a broad ph optimum with maximum activity at approx. ph 6.0 and a temperature optimum of 50 degrees c. the ph of elution f ... | 1988 | 3063255 |
| storage of human platelet concentrates in an artificial medium without dextrose. | it was shown previously that human blood platelets stored in an artificial medium (pcd) for up to 5 days remain functional in vitro and have normal survival and recovery in vivo. this report demonstrates that the medium can be simplified further by the removal of dextrose, leaving for study a medium consisting simply of balanced salts and citrate anticoagulant (pc). some dextrose, 3.2 mm, was present in the fresh pc platelet concentrates due to plasma carryover in the production of platelet conc ... | 1988 | 3368933 |
| purification and properties of an acetylxylan esterase from fibrobacter succinogenes s85. | an acetylxylan esterase (ec 3.1.1.6) was purified to apparent homogeneity from the nonsedimentable extracellular culture fluid of fibrobacter succinogenes s85 grown on cellulose. this enzyme had an apparent molecular mass of 55 kda and an isoelectric point of 4.0. the temperature and ph optima were 45 degrees c and 7.0, respectively. the apparent km and vmax were 2.7 mm and 9,100 u/mg, respectively, for the hydrolysis of alpha-naphthyl acetate. the enzyme cleaved acetyl residues from birchwood a ... | 1990 | 2082827 |
| reversal of pulmonary hypoxic vasoconstriction with pentoxifylline and aminophylline in isolated lungs. | pentoxifylline (pent) is a xanthine known to improve erythrocyte deformability and thought to have little effect on smooth muscle tone. in this study i examined the direct effects of pent on the pulmonary vasculature of isolated lungs and compared them with the effects of aminophylline. the object was to study whether pent can reverse the hypoxic pressor response (hpr) by its hemorheological property. changes in pulmonary arterial pressure (pa) of isolated lungs (pigs and rats) perfused at const ... | 1988 | 3370545 |
| serological and morphological observation on the outer membrane of bacteroides succinogenes. | | 1990 | 2107350 |
| purification and characterization of a chloride-stimulated cellobiosidase from bacteroides succinogenes s85. | a cellobiosidase with unique characteristics from the extracellular culture fluid of the anaerobic gram-negative cellulolytic rumen bacterium bacteroides succinogenes grown on microcrystalline cellulose (avicel) in a continuous culture system was purified to homogeneity by column chromatography. the enzyme was a glycoprotein with a molecular weight of approximately 75,000 and an isoelectric point of 6.7. when assayed at 39 degrees c and ph 6.5, the activity of the enzyme with p-nitrophenyl-beta- ... | 1988 | 3384799 |
| site-specific rate constants for iron removal from diferric transferrin by nitrilotris(methylenephosphonic acid) and pyrophosphate. | the kinetics of iron removal from human serum diferric transferrin by nitrilotris(methylenephosphonic acid) (ntp) and pyrophosphate (ppi) have been studied in 0.1 m, ph 7.4, n-(2-hydroxyethyl)piperazine-n'-2-ethanesulfonate buffer at 25 degrees c using urea/polyacrylamide gel electrophoresis. the four microscopic rate constants required for a complete description of iron removal from the two transferrin metal-binding sites have been measured at 100 mm concentrations of ntp and ppi. there is very ... | 1990 | 2168158 |
| [determination of intracellular concentration of h+, na+, k+ and atp in bacteroides succinogenes adapted to monensin]. | the internal concentrations of the cations h+, na+, k+ and the intracellular atp concentration were measured on b succinogenes adapted to the presence of the ionophore monensin (0.5 microm). adapted bacteria were still able to regulate the intracellular concentrations of na+ and k+, but their internal ph and atp concentration were lower than in control bacteria. | 1990 | 2169758 |
| dna sequence of a fibrobacter succinogenes mixed-linkage beta-glucanase (1,3-1,4-beta-d-glucan 4-glucanohydrolase) gene. | the dna sequence of a mixed-linkage beta-glucanase (1,3-1,4-beta-d-glucan 4-glucanohydrolase [ec 3.2.1.73]) gene from fibrobacter succinogenes cloned in escherichia coli was determined. the general features of this gene are very similar to the consensus features for other gram-negative bacterial genes. the gene product was processed for export in e. coli. there is a high level of sequence homology between the structure of this glucanase and the structure of a mixed-linkage beta-glucanase from ba ... | 1990 | 2193918 |
| effects of potassium ion concentrations on the antimicrobial activities of ionophores against ruminal anaerobes. | the antimicrobial activities of monensin and lasalocid against representative strains of ruminal bacteria were evaluated in medium containing three different concentrations of potassium (1.3, 7.9, or 23.3 mm). the growth of eubacterium ruminantium was inhibited by low concentrations of ionophores (less than or equal to 0.16 mg/liter), while the strain of streptococcus bovis tested was resistant to high concentrations of ionophores (40 mg/liter) at all potassium concentrations tested. the mics of ... | 1987 | 3426214 |
| molecular mimicry in reiter's syndrome: cytotoxicity and elisa studies of hla-microbial relationships. | the pathogenic links between hla antigens, certain bacterial infections and arthritis have not yet been characterized. the hypothesis of cross-reactivity between hla b27, the marker of disease susceptibility for these disorders, and the provocative microorganism has been suggested by studies of klebsiella and ankylosing spondylitis. the present study examines the possibility of molecular mimicry between hla b27 and two organisms implicated more directly in reactive arthritis, yersinia enterocoli ... | 1986 | 3488263 |
| cellulose digestion and cellulase regulation and distribution in fibrobacter succinogenes subsp. succinogenes s85. | fibrobacter succinogenes subsp. succinogenes s85 initiated growth on microcrystalline cellulose without a lag whether inoculated from a glucose, cellobiose, or cellulose culture. during growth on cellulose, there was no accumulation of soluble carbohydrate. when the growth medium contained either glucose or cellobiose in combination with microcrystalline cellulose, there was a lag in cellulose digestion until all of the soluble sugar had been utilized, suggesting an end product feedback mechanis ... | 1990 | 2339881 |
| catalytic and substrate-binding domains of endoglucanase 2 from bacteroides succinogenes. | endoglucanase 2 (eg2) of the cellulolytic ruminal anaerobe bacteroides succinogenes is a 118-kilodalton (kda) enzyme which binds to cellulose and produces cellotetraose as the end product of hydrolysis. the purified enzyme was treated with the protease trypsin in an attempt to isolate peptides which retained the ability to either hydrolyze soluble carboxymethyl cellulose or bind to insoluble cellulose. there was no loss in endoglucanase activity (carboxymethylcellulase) over a period of 2 h foll ... | 1989 | 2470726 |
| development of the cellulolytic microflora in the rumen of lambs transferred into sterile isolators a few days after birth. | seven lambs, separated from their dam 24 h after birth, were kept in a conventional environment until transferred to sterile isolators between 1 and 9 days of age: two on day 1 (ia and ib), two on day 4 (iva and ivb), one on day 8 (viiia), and two on day 9 (ixa and ixb). the lambs were reared in these isolators until 120 days of age. lambs ia, ib, ixa, and ixb were free of cellulolytic bacteria when they were placed in the isolators. they were then inoculated with bacteroides succinogenes s85 wh ... | 1989 | 2731080 |
| effect of season and lactation on postpartum fertility of polypay, dorset, st. croix and targhee ewes. | the objective of this study was to compare rebreeding activities of spring- vs fall-lambing polypay, dorset, st. croix and targhee ewes that either suckled their lambs for 40 d or had lambs weaned at birth. seasonal effects of male fertility were reduced by utilizing an excess number of fertile rams in the spring. plasma concentrations of progesterone were monitored to assess days to the first normal ovulation, days to conception and estrous vs anestrous activity. breed, season and lactation aff ... | 1989 | 2737977 |
| the dilation of arterioles in frog submaxillaris muscle caused by visible light. | transillumination of submaxillaris muscle with incandenscent lamp led to arteriolar dilation if the illuminance exceeded 1-2 x 10(3) lux or reached the time-mean value of 3 x 10(4) lux when a flash lamp was used (pulse duration 10 microseconds, repetition rate 50 s-1). the temperature increase within a light spot on the muscle did not exceed 0.1 degrees c, visible light being the only reason of the dilation of arterioles. at 6.3 x 10(4) lux (incandescent lamp, green filter) the latency of dilati ... | 1989 | 2786511 |
| biological activity of a new neuronal growth factor from injured peripheral nerve. | in response to transection injury, the distal nerve segment produces a soluble neurite promoting factor (sn). in this study, the ability to support neuronal survival and differentiation have been studied. embryonic rat dorsal root ganglion (drg) neurons were plated out on collagen substrates and incubated in medium containing either sn or nerve growth factor (ngf). the number of surviving neurons was counted after 1, 2, 4, 7, and 15 days in vitro. after fixation and staining, the diameter of the ... | 1989 | 2805333 |
| [therapy of aids]. | therapeutic strategies in the management of aids should aim at an inhibition of the replication and spread of hiv; the reconstitution of immune functions; an effective chemotherapy of opportunistic infections and tumors; and the prevention of new opportunistic infections. at present, these goals can be only partially achieved and where beneficial therapeutic results are obtained they are only transient in nature. antiretroviral therapy and chemotherapy of infections have, however, resulted in so ... | 1988 | 2849247 |
| sulfite oxidase from chicken liver. the role of imidazole and carboxyl groups for the reaction with cytochrome c. | oxidation of sulfite to sulfate by sulfite oxidase is inhibited when the enzyme is treated with reagents known to modify imidazole and carboxyl groups. modification inhibits the oxidation of sulfite by the physiological electron acceptor cytochrome c, but not by the artificial acceptor ferricyanide. this indicates interference with reaction steps that follow the oxidation of sulfite by the enzyme's molybdenum cofactor. reaction with diethylpyrocarbonate modifies ten histidines per enzyme monomer ... | 1986 | 3019695 |
| sulfite oxidase from chicken liver. the role of imidazole and carboxyl groups for the reaction with cytochrome c. | oxidation of sulfite to sulfate by sulfite oxidase is inhibited when the enzyme is treated with reagents known to modify imidazole and carboxyl groups. modification inhibits the oxidation of sulfite by the physiological electron acceptor cytochrome c, but not by the artificial acceptor ferricyanide. this indicates interference with reaction steps that follow the oxidation of sulfite by the enzyme's molybdenum cofactor. reaction with diethylpyrocarbonate modifies ten histidines per enzyme monomer ... | 1986 | 3019695 |
| alpha-adrenoceptor responsiveness in the aged rat. | the effects of ageing on vascular alpha 1- and alpha 2-adrenoceptors were examined using anaesthetised and pithed young (3-7 months) and old (21-24 months) sprague-dawley rats. in pithed animals, the pressor and cardioinhibitory effects of the alpha 2-adrenoceptor agonist xylazine were significantly reduced in old animals (8- and 6-fold shift), but the pressor effects of the alpha 1-adrenoceptor agonist amidephrine were not significantly altered by ageing. in anaesthetised rats, the pressor resp ... | 1986 | 3019712 |
| effect of phenolic monomers on the growth and beta-glucosidase activity of bacteroides ruminicola and on the carboxymethylcellulase, beta-glucosidase, and xylanase activities of bacteroides succinogenes. | trans-p-coumaric acid inhibited the growth of bacteroides ruminicola on both cellobiose and glucose, while trans-ferulic acid and vanillin retarded growth. the phenolic monomers varied in their potential to inhibit the bacteroides succinogenes beta-glucosidase, carboxymethylcellulase, and xylanase, with p-coumaric acid being the most inhibitory. the b. ruminicola beta-glucosidase was inhibited less than 10% by all three compounds. | 1988 | 3146948 |
| establishment of bacteroides succinogenes and measurement of the main digestive parameters in the rumen of gnotoxenic lambs. | we attempted to determine the degree of diversification of the microflora that allow the establishment of bacteroides succinogenes s85 in the rumen of gnotoxenic lambs. four lambs (group i) received an inoculum orally, composed of 182 noncellulolytic bacterial strains (inoculum 1) previously isolated from the rumen of conventional young lambs. two lambs (group ii) were inoculated with 32 strains (inoculum 2) selected among the 182 strains of inoculum 1. two lambs (group iii) received an inoculum ... | 1988 | 3208206 |
| alignment of cilia in immotile-cilia syndrome. | alignment of cilia in nasal epithelial cells from eight human subjects suffering from immotile-cilia syndrome was compared with that of cells from five control subjects. individual cilia were assessed according to the orientation of their basal feet. the range of orientation of basal feet on a single cell varied from 26 degrees to 261 degrees and 54 degrees to 275 degrees in controls and patients respectively. less than 10% of the cells from each group supported cilia that were aligned randomly. ... | 1986 | 3750315 |
| toxic shock syndrome associated with tsst-1 producing coagulase-negative staphylococci. | coagulase-positive staphylococci have been isolated from more than 95% of cases of toxic shock syndrome (tss). toxic shock syndrome toxin-1 (tsst-1) is produced in more than 90% of cases. a case is described of recurrent tss in which a coagulase-negative staphylococcus was isolated from the vagina and produced tsst-1. no coagulase-positive staphylococci were isolated. coagulase-negative staphylococci should be considered as causative agents in cases of tss. assay for tsst-1 should be performed o ... | 1986 | 3777014 |
| phosphoenolpyruvate-dependent phosphorylation of hexoses by ruminal bacteria: evidence for the phosphotransferase transport system. | six species of ruminal bacteria were surveyed for the phosphoenolpyruvate (pep)-dependent phosphorylation of glucose. selenomonas ruminantium hd4, streptococcus bovis jb1, and megasphaera elsdenii b159 all showed significant activity, but butyrivibrio fibrisolvens 49, bacteroides succinogenes s85, and bacteroides ruminicola b1(4) showed low rates of pep-dependent phosphorylation and much higher rates in the presence of atp. s. ruminantium hd4, s. bovis jb1, and m. elsdenii b159 also used pep to ... | 1986 | 3789722 |
| hemodynamic effects of sodium in uremia. | | 1987 | 3794516 |
| detection of cellulose-binding proteins in electrophoresis gels by filter paper affinity blotting. | proteins separated in electrophoresis gels were tested for the ability to bind cellulose by a simple blotting procedure. proteins were blotted onto whatman no. 1 filter paper by diffusion or by electrophoretic transfer and detected by coomassie blue staining. certain proteins released into culture supernatant by bacteroides succinogenes nr9 (atcc 43854) adhered strongly to cellulose, but were not found to have carboxymethylcellulose activity. boiling of samples prior to electrophoresis eliminate ... | 1988 | 3218733 |
| ministry animal health schemes. | | 1986 | 3811186 |
| effects of alkaline hydrogen peroxide treatment on in vitro degradation of cellulosic substrates by mixed ruminal microorganisms and bacteroides succinogenes s85. | the effects of sodium hydroxide (naoh) and alkaline hydrogen peroxide (ahp) treatments on wheat straw (ws) and various cellulosic substrates were determined by measuring susceptibility to degradation by mixed ruminal organisms or bacteroides succinogenes s85. in vitro incubations were used to measure differences in fermentation resulting from each successive step in the ahp treatment process. in vitro incubations through 48 or 108 h were conducted to measure these differences. the ahp treatment ... | 1988 | 3291761 |
| hemorrhagic shock induces bacterial translocation from the gut. | sepsis and multiple organ failure are common after hemorrhagic shock. the goal of the current experiments was to determine whether hemorrhagic shock would promote the translocation of bacteria from the gut to visceral organs. twenty-four hours after being subjected to sham shock, or 30, 60, or 90 minutes of shock (30 mm hg), rats were sacrificed and their organs quantitatively cultured for translocating bacteria. there was a direct relationship between the duration of hemorrhagic shock and the 2 ... | 1988 | 3294427 |
| asexual multiplication of tetrathyridia of mesocestoides corti in crotalus viridis viridis. | prairie rattlesnakes, crotalus viridis viridis rafinesque, 1818, were infected with tetrathyridia of mesocestoides corti hoeppli, 1925, by means of oral intubation. snakes were maintained at constant temperatures of either 25, 30, or 35 c for 4-24 wk. postmortem examinations revealed the first experimental evidence of asexual multiplication of tetrathyridia in a laboratory-infected ectothermic host. time and temperature were statistically significant with p values of less than 0.05. | 1985 | 3981738 |
| the effect of ammonia treatment on the solubilization of straw and the growth of cellulolytic rumen bacteria. | pre-treatment of straw with anhydrous ammonia increased its susceptibility to solubilization by the predominant cellulolytic bacteria from the rumen, bacteroides succinogenes, ruminococcus albus and r. flavefaciens. ammonia treatment also increased the production of microbial protein and fermentation products by all three species. scanning electron microscope observations of straw during digestion suggested that the attack of straw by these bacteria was accompanied by the formation of substantia ... | 1985 | 3997690 |
| intracoronary naloxone in hemorrhagic shock: dose-dependent stereospecific effects. | treatment with naloxone improves cardiovascular function and survival in a variety of shock models, and numerous sites and mechanisms for its action have been proposed. data presented in this article support the hypothesis that in hemorrhagic shock naloxone exerts its beneficial hemodynamic effects by acting primarily at cardiac opiate receptors. naloxone or its stereoisomer (d-naloxone) were administered intravenously (iv) and directly into the coronary circulation (ic) in dogs anesthetized wit ... | 1985 | 4025562 |
| the human met oncogene is a member of the tyrosine kinase family. | prolonged exposure of a nontumorigenic human osteogenic sarcoma cell line (hos) with the direct acting carcinogen n-methyl-n'-nitro-n-nitrosoguanidine (mnng) gave rise to morphologically transformed cells which were tumorigenic in nude mice and termed mnng-hos. we have shown that dna from mnng-hos cells will transform nih/3t3 cells and have isolated greater than 35 kb of human dna containing an oncogene, termed met. the activated met oncogene expresses a novel 5.0 kb rna transcript which is a hy ... | 1986 | 3332011 |
| [significance of bordetella bronchiseptica and pasteurella multocida in rhinitis atrophicans suum]. | | 1985 | 4072490 |
| chromosome analysis of human oocytes and embryos: does delayed fertilization increase chromosome imbalance? | thirty per cent of a sample of 120 unfertilized human oocytes carried chromosome abnormalities highly correlated with maternal age (38% in patients greater than 35, as compared with 24% in younger patients). fertilized eggs, when observed 17 h after insemination, showed in 1.6% a single pronucleus suggesting parthenogenetic activation. in 92% of the cases two pronuclei were observed and the rate of chromosome anomalies depended on the morphological aspect of the embryos. triploidy was also encou ... | 1988 | 3350934 |
| ultrastructural observations on the sporogony of eimeria maxima. | | 1985 | 4093234 |
| electrophoretic studies on renin substrate from various species. | | 1971 | 4100516 |
| peptides from the site of rabbit antibody: anti-3-azopyridine antibody. | | 1971 | 4106096 |
| spore appendages and taxonomy of clostridium sordellii. | twenty-five strains of clostridium sordellii were divided into two groups on the basis of spore fine structure. sixteen strains formed spores with smooth tubular appendages, and nine strains formed spores which lacked appendages. the other properties of the 25 strains were relatively constant. since the minor strain variability which was encountered did not correlate with spore appendage status, fragmentation of this species on the basis of spore appendage status is not advocated. | 1971 | 4109864 |
| [the effect of colchicine and aurantin on rosette-forming cells in the secondary immunologic response to administration of the vi-antigen of typhoid bacteria]. | | 1972 | 4117531 |
| synaptic patterns in the visual cortex of the cat and monkey. electron microscopy of golgi preparations. | | 1973 | 4124647 |
| cell envelope morphology of rumen bacteria. | the cell walls of three species of rumen bacteria (bacteroides ruminicola, bacteroides succinogenes, and megasphaera elsdenii) were studied by a variety of morphological methods. although all the cells studied were gram-negative and had typical cytoplasmic membranes and outer membranes, great variation was observed in the thickness of their peptidoglycan layers. megasphaera elsdenii evidenced a phenomenally thick peptidoglycan layer whose participation in septum formation was very clearly seen. ... | 1974 | 4133611 |
| ultrastructural study of membrane-bound enzymes in thymocytes. | | 1974 | 4140869 |
| effect of psychopharmacological agents on the gonads of female rats. | | 1974 | 4153333 |
| [leptotrichia buccalis--culture, biochemistry and antibiotics]. | | 1974 | 4154653 |
| further investigation of specific and non-specific adsorption of serum globulins to staphylococcus aureus. | | 1968 | 4177124 |
| in vivo effect of lsd, morphine, ethanol and delta9-tetrahydrocannabinol on mouse brain adenosine triphosphatase activity. | | 1974 | 4272745 |
| in vivo effect of lsd, morphine, ethanol and delta9-tetrahydrocannabinol on mouse brain adenosine triphosphatase activity. | | 1974 | 4272745 |
| [cytochromes]. | | 1965 | 4287863 |
| [the effect of various gamma aminobutyric acid doses on the total and individual phospholipid levels in whole blood entering and leaving the brain]. | | 1968 | 4300822 |
| pyrophosphate enhancement of hypocalcemic effect of thyrocalcitonin in rat. | | 1969 | 4309707 |
| pyrophosphate enhancement of hypocalcemic effect of thyrocalcitonin in rat. | | 1969 | 4309707 |
| preparation of immune rabbit sera with neutralizing activity against human cytomegalovirus and varicella-zoster virus. | | 1970 | 4316778 |
| a temporal and structural relationship between viral rna polymerase and nicotinamide adenine dinucleotide pyrophosphorylase in the cytoplasm of krebs-2 ascites cells infected with encephalomyocarditis virus. | | 1968 | 4329661 |
| postweaning development of negative feedback in the pituitary-adrenal system of the rat. | | 1973 | 4353346 |
| postweaning development of negative feedback in the pituitary-adrenal system of the rat. | | 1973 | 4353346 |
| quantitative extraction of adenosine triphosphate from cultivable and host-grown microbes: calculation of adenosine triphosphate pools. | existing data on adenosine triphosphate (atp) pools in microbes are deficient for two reasons: (i) incomplete extractions of atp, and (ii) the failure to take into account that the adverse effects of extracting procedures on standard atp exert analogous effects on the atp released from bacterial cells. methods for correcting observed yields and calculating atp pools have been demonstrated. three bacterial species were used in the studies on extraction of atp: escherichia coli, mycobacterium phle ... | 1973 | 4356463 |
| the synthesis of encephalomyocarditis virus polypeptides in infected l-cells and cell-free systems. | | 1974 | 4369366 |
| enzymatic studies of pure cultures of rumen microorganisms. | joyner, a. e., jr. (university of california, davis), and r. l. baldwin. enzymatic studies of pure cultures of rumen microorganisms. j. bacteriol. 92:1321-1330. 1966.-the activities of enzymes representing the major pathways of carbohydrate metabolism and anaerobic electron transport in cell-free extracts of whole rumen contents have been reported. the effects of diet upon the activities of several enzymes suggested that enzymatic measurements might prove useful for the study of rumen metabolism ... | 1966 | 4380801 |
| prolongation of canine renal homograft survival by post-transplantation treatment with antithymocyte serum. | | 1970 | 4392297 |
| value of electrical pacing and beta adrenergic blockers in the prevention of fatal ventricular arrhythmias. | | 1971 | 4399445 |
| value of electrical pacing and beta adrenergic blockers in the prevention of fatal ventricular arrhythmias. | | 1971 | 4399445 |
| the influence of stress on the survival of experimental skin flaps. a study on rats. | | 1972 | 4405684 |
| comparison of the sphingolipid content of rumen bacteroides species. | ten strains of bacteroides ruminicola were found to contain phosphosphingolipids. four strains of bacteroides amylophilus and one strain each of bacteroides succinogenes and bacteroides sp. were devoid of phosphosphingolipids. | 1974 | 4476196 |
| regulation of bacteriophage t5 development by coli factors. | the i-type colicinogenic factor colib transforms escherichia coli from a permissive to a nonpermissive host for bacteriophage t5 reproduction by preventing complete expression of the phage genome. t5-infected colib(+) cells synthesize only class i (early) phage protein and ribonucleic acid (rna). neither phage-specific class ii proteins [associated with viral deoxyribonucleic acid (dna) replication] nor class iii proteins (phage structural components) are formed due to the failure of the infecte ... | 1972 | 4554465 |
| recall of antibody synthesis to the primary antigen following successive immunization with heterologous albumins. a two-cell theory of the original antigenic sin. | | 1972 | 4563348 |
| the construction of lambda transducing phages containing deletions defining regulatory elements of the lac and trp operons in e. coli. | | 1974 | 4601264 |
| spinal projections of renal afferent nerves in the rat. | this study was designed to describe renal afferent information with respect to its intraspinal projections, convergence with cutaneous inputs, ascending projections, and modulation by descending fiber tracts. extracellular recordings were made from neurons in the spinal gray while electrically stimulating the renal nerves in chloralose-anesthetized, artificially ventilated rats. almost all neurons (n = 119) were spontaneously active. some responses consisted of high-frequency bursts while others ... | 1988 | 3370480 |
| gene-specific differences in the aflatoxin b1 adduction of chicken erythrocyte chromatin. | mature and immature chicken erythrocyte nuclei were treated with activated aflatoxin b1 (2,3-dichloroaflatoxin b1), producing covalently bound dna adducts. this reaction produces alkali-labile sites in the dna which can be identified by using a variation of the maxam-gilbert sequencing procedure. we determined the aflatoxin b1 accessibility of defined regions of the erythroid genome by using different specific probes and monitoring the disappearance of similar-sized fragments generated by restri ... | 1988 | 3142684 |
| cloning and expression of a bacteroides succinogenes mixed-linkage beta-glucanase (1,3-1,4-beta-d-glucan 4-glucanohydrolase) gene in escherichia coli. | a pseudorandom genomic library of bacteroides succinogenes dna, cloned into puc8 in escherichia coli, was screened for beta-glucanase activity on 0.1% lichenan plates. six high-activity clones, containing identical 5.2-kilobase inserts of b. succinogenes dna, were obtained. the clones exhibited activity solely on beta-glucan substrates containing beta-(1----3)(1----4) linkages, thus manifesting a specific fibrolytic enzyme previously unrecognized in b. succinogenes. a subclone (pji10) of the ori ... | 1988 | 3145707 |
| 18q+, the progeny of a balanced translocation t(1;18)mat: case report with necropsy findings. | a female infant with additional genetic material on the long arm of chromosome 18 is described. cytogenetic studies of the infant and her mother showed that the altered region resulted from an unbalanced translocation of part of the long arm of chromosome 1. this chromosomal abnormality has not been reported previously, according to a recent registry of abnormal chromosome patterns. the patient had hydrops fetalis and multiple congenital abnormalities, involving the cardiovascular, respiratory, ... | 1986 | 3723558 |
| glucose uptake by the cellulolytic ruminal anaerobe bacteroides succinogenes. | glucose uptake by bacteroides succinogenes s85 was measured under conditions that maintained anaerobiosis and osmotic stability. uptake was inhibited by compounds which interfere with electron transport systems, maintenance of proton or metal ion gradients, or atp synthesis. the most potent inhibitors were proton and metal ionophores. oxygen strongly inhibited glucose uptake. na+ and li+, but not k+, stimulated glucose uptake. a variety of sugars, including alpha-methylglucoside, did not inhibit ... | 1987 | 3804970 |
| an appraisal of the technique of polymorphonuclear leukocyte chemiluminescence as a means to detect compounds with antiinflammatory activity. | the measurement of luminol-dependent chemiluminescence of inflammatory rat polymorphonuclear leukocytes (pmns), when exposed to opsonized zymosan particles, has been used as a possible screening system for nonsteroidal antiinflammatory drugs (nsaid). an in vitro system may be used to detect nsaids, however the sensitivity is not of a high order. on the other hand an inhibitor of the lipoxygenase system (ndga) and a dual lipo-cyclooxygenase inhibitor (bw755c) are easily detectable at low concentr ... | 1985 | 3935877 |
| purification and properties of a protein activator of phosphorylated branched-chain 2-oxo acid dehydrogenase complex. | the protein activator of phosphorylated branched-chain 2-oxo acid dehydrogenase complex was purified greater than 1000-fold from extracts of rat liver mitochondria; the specific activity was greater than 1000 units/mg of protein (1 unit gives half-maximum re-activation of 10 munits of phosphorylated complex). sodium dodecyl sulphate/polyacrylamide-gel electrophoresis gave two bands (mr 47700 and 35300) indistinguishable from the alpha- and beta-subunits of the branched-chain dehydrogenase compon ... | 1985 | 3977842 |
| x-ray tv system for measuring microcirculation in small pulmonary vessels. | we developed a new system that consists of 1) a specially designed x-ray apparatus, 2) an x-ray-sensitive 1-in. vidicon camera, and 3) a digital image-processing device. the picture element is approximately 20 micron in size, and the time required for one frame is 1/30 s. using this system, we measured the internal diameter (id), the cross-sectional area, flow velocity, volume flow, and transit time of small pulmonary vessels of approximately 100-500 micron at control and with serotonin in anest ... | 1985 | 4055558 |
| [data providing a basis for the mpel of tetrahydrophthalic anhydride, tetrahydrophthalamide and hydroxymethyltetrahydrophthalimide in the air of the work environment]. | | 1985 | 4092972 |
| isolation and characterization of endoglucanases 1 and 2 from bacteroides succinogenes s85. | two endoglucanases designated eg1 and eg2 were purified by column chromatography from the nonsedimentable extracellular culture fluid of bacteroides succinogenes s85. they accounted for approximately 32 and 11%, respectively, of the total endoglucanase present in the nonsedimentable fraction. the most active enzyme (eg1) had a molecular weight of 65,000, pi of 4.8, and temperature and ph optima of 39 degrees c and 6.4, respectively. the km for carboxymethyl cellulose was 3.6 mg/ml, and the vmax ... | 1988 | 3384798 |
| use of phylogenetically based hybridization probes for studies of ruminal microbial ecology. | to address the long-standing need for more precise descriptions of natural microbial ecosystems, 16s rrnas were used to track certain species and phylogenetically coherent groups of microorganisms in their natural setting without culturing. species- and group-specific 16s rrna-targeted oligonucleotide hybridization probes were developed to enumerate various strains of bacteroides succinogenes and lachnospira multiparus-like organisms in the bovine rumen before, during, and after perturbation of ... | 1988 | 3389805 |
| satellite cells in the regenerated and regrafted skeletal muscles of rats. | soleus (sol) muscles were grafted into extensor digitorum longus (edl) muscle beds (edl-first-graft). sixty days later, some mature edl-first-grafts were regrafted into their own beds (edl-second-grafts). fully regenerated muscle fibers and satellite cells were observed in both types of mature grafts. the ratios of satellite cell nuclei per total nuclei (myonuclei and satellite cell nuclei) were 4.81 +/- 0.47% for edl-2nd graft, 4.26 +/- 0.51% for edl-1st-graft, 4.30 +/- 0.33% for control sol, a ... | 1988 | 3396655 |
| the effect of tissue culture agar on chromosome breakage, sister-chromatid exchanges and clonogenicity in human cells. | to investigate the cytogenetic effects of electromagnetic fields, a system containing an agar gel was developed to support the growth of various human cell types (peripheral lymphocytes, lymphoblasts, and fibroblasts). when compared to alioquots of identical cells, grown in plastic culture vessels, statistically significant increases in the frequencies of chromosome breakage, sister-chromatid exchange and decreased cloning efficiency were observed in those cells cultured in the agar. these resul ... | 1988 | 3398870 |
| in vitro reversal of cardiac deterioration in septic shock with tetraethylammonium chloride. | overwhelming sepsis associated with cardiac failure continues to be a major clinical problem. this is commonly associated with a failure to respond to conventional pharmacologic therapy. this study was undertaken to see if manipulations of the electrophysiologic defects previously described by treatment with tetraethylammonium chloride (tea) would be advantageous. septic shock was induced in rabbits by a lethal dose of escherichia coli. peak tension and velocities of contraction and relaxation w ... | 1986 | 3510606 |
| fodrin is part of a filamentous cortical sheath of the detergent resistant cytoskeleton of cultured cells before and after cytochalasin treatment. | cytoskeletons of cultured cells prepared under mild conditions in the presence of "stabilization' buffer contain most of the fodrin present in the cells. the fodrin in these cytoskeletons was localized by immunofluorescence microscopy and found to be present in a cortical sheath of fine filaments. in general, the filamentous distribution showed no correspondence with actin bundles as revealed by double-label fluorescence microscopy. however, in cells with large and abundant stress fibers, some c ... | 1986 | 3514218 |