| cell type-specific expression of the human transferrin gene. role of promoter, negative, and enhancer elements. | transferrin (tf), the iron transport protein of vertebrate serum, is mainly synthesized in the liver. cis-acting dna elements required for liver-specific expression of the human tf gene were identified by transient and stable expression assays in human hepatoma (hepg2 and hep3b) and epithelial carcinoma (hela) cell lines. deletion analysis of the 5' dna sequences of the gene have defined four functionally different regions: (a) a cell type-specific promoter located between positions -125 and -45 ... | 1989 | 2708361 |
| protein kinase c is activated in glomeruli from streptozotocin diabetic rats. possible mediation by glucose. | glomerular inositol content and the turnover of polyphosphoinositides was reduced by 58% in 1-2 wk streptozotocin diabetic rats. addition of inositol to the incubation medium increased polyphosphoinositide turnover in glomeruli from diabetic rats to control values. despite the reduction in inositol content and polyphosphoinositide turnover, protein kinase c was activated in glomeruli from diabetic rats, as assessed by an increase in the percentage of enzyme activity associated with the particula ... | 1989 | 2708528 |
| [anaerobic bacteria in chronic renal insufficiency: apropos of a case]. | we relate a case of chronic renal insufficiency complicated by urinary anaerobic infection caused by bacteroides vulgatus and peptococcus asaccharolyticus. | 1989 | 2489402 |
| comparative in vitro activity of cefoxitin, cefotaxime alone, and in combination with desacetylcefotaxime against the bacteroides species. | the agar dilution method was used to determine the inhibitory activity of cefotaxime (ctx) alone, desacetylcefotaxime (dctx) alone, ctx plus dctx, and cefoxitin against 74 clinical isolates of the bacteroides species recovered from diabetic patients with foot ulcers. the study concluded that the addition of dctx to ctx increased the inhibitory activity from 45% to 73% for all strains tested and from 50% to 81% among the 32 strains of bacteroides fragilis. this synergistic interaction against b. ... | 1989 | 2714070 |
| degradation of intestinal glycoproteins by bacteroides vulgatus. | bacteroides vulgatus, isolated from a patient with crohn's disease, produced in gnotobiotic rats 7 constitutive enzymes that might be concerned with the degradation of intestinal glycoproteins. furthermore bacteroides vulgatus caused an almost complete loss of blood group antigenicity of the intestinal glycoproteins. enzymes with the potency to release toxic compounds from hepatic conjugates and plant glycosides, beta-glucuronidase and beta-glucosidase, respectively, were only detectable in smal ... | 1989 | 2721924 |
| role of starch as a substrate for bacteroides vulgatus growing in the human colon. | bacteroides vulgatus is the numerically predominant bacteroides species in the human colonic microflora. unlike other colonic bacteroides species, b. vulgatus is not a versatile utilizer of polysaccharides. the only types of polysaccharide that support rapid growth and high growth yields by all strains are the starches amylose and amylopectin. amylase and alpha-glucosidase activities are among the highest found in a bacterial fraction obtained from human feces. this observation raised the questi ... | 1988 | 2460027 |
| identification by c-banding of two human prostate tumour cell lines, 1013l and du 145. | two human prostate carcinoma cell lines are easily distinguished by c-banding. du 145 has one metacentric chromosome with a large centromeric band, 1 metacentric and 2 acrocentric chromosomes with interstitial c-bands. the y chromosome was present in 48% and 42% of the cells at passages 83 and 106, respectively, whereas in 1013l only one metacentric chromosome contained distinct extra c bands--one at the centromere and one interstitially. the y chromosome was present in 72% of the cells at passa ... | 1989 | 2744886 |
| risk factors for kaposi's sarcoma in the vancouver lymphadenopathy-aids study. | in our ongoing cohort study of homosexual men, the ratio of new kaposi's sarcoma (ks) cases to new opportunistic infections (oi) during the periods 1982-1985, 1986-1987, and 1988-1989 fell from 0.75 (9 ks: 12 oi) to 0.57 (12 ks:21 oi) to 0.27 (4 ks:15 oi), respectively. to examine factors associated with the development of ks as compared to oi, we compared antecedent risk factors in 25 ks cases and 48 oi "controls." in univariate analyses, several classical hiv risk factors including numbers of ... | 1990 | 2395081 |
| antibody responses and protection in mice immunized with herpes simplex virus type 1 antigen immune-stimulating complex preparations. | the formation of immune-stimulating complexes (iscoms) obtained by mixing the glycoside quil a with an antigen preparation derived from herpes simplex virus type 1 (hsv-1)-infected cell cultures using a zwitterionic detergent is described. the hsv-1 antigen preparation incorporated into iscoms elicited significantly greater antibody responses in mice than the same preparation administered together with aluminium hydroxide gel, and provided complete protection against hsv-1 or hsv-2 lethal, syste ... | 1989 | 2769234 |
| dietary modification of potential vitamin k supply from enteric bacterial menaquinones in rats. | rats given a low-fibre diet based on boiled white rice developed symptoms of severe vitamin k deficiency within 23 d. inclusion of autoclaved black-eye beans (vigna unguiculata) in the diet prevented the bleeding syndrome. to test the hypothesis that deficiency resulted from low phylloquinone intake exacerbated by inadequate production of menaquinones by the enteric bacteria, a follow-up experiment was carried out in which groups of rats were given an all-rice diet, a rice + beans diet or a stoc ... | 1990 | 2383538 |
| experimental studies on some effects of prostaglandin e2 in acute renal failure. | acute renal failure has been produced in rats with oral administration of uranyl nitrate 40 mg/kg of body weight once, and histopathological changes in the kidneys with blood biochemical values were studied by sacrificing them after 36 hours of feeding. prostaglandin e2 in 100 micrograms/kg of body weight was orally administered to another group of rats along with uranyl nitrate 40 mg/kg of body weight once and sacrificed them 36 hours after feeding. it was found that prostaglandin e2 has some p ... | 1990 | 2362130 |
| [differentiated changes in the activity of the sympathetic nerves induced by glycopenia in spontaneously hypertensive rats]. | 2-deoxy-d-glucose (2-dg) evokes an increase in the efferent discharge rate of the adrenal nerve in anesthetized rats and rabbits. a 63% increase in adrenal sympathetic nerve activity (adrsna) occurred within 15 min after 125 mg/kg 2-dg administration in alert animals, no changes being revealed in the renal sympathetic nerve activity (rensna), blood pressure (bp) or the heart rate (hr). additional administration of 375 mg/kg 2-dg (cumulative dose 500 mg/kg) led to an over 120% increase in the adr ... | 1989 | 2806664 |
| application of brain microdialysis to study the pharmacology of the 5-ht1a autoreceptor. | 5-hydroxytryptamine (5-ht) receptors of the 5-ht1a subtype are localized on serotoninergic cells and dendrites in the raphe nuclei of the brain stem and are believed to regulate synaptic 5-ht release through an inhibitory influence on serotoninergic impulse flow. the effects of 5-ht1a agonists on 5-ht release can, therefore, only be detected by measurement of 5-ht release from intact serotoninergic neurones. here we review the evidence that the microdialysis technique, when applied to the anaest ... | 1990 | 2259248 |
| bacterial growth control studied by flow cytometry. | by employing flow cytometry, the dna content and cell size of individual bacterial cells may be determined rapidly and with high precision. also, the number of dna replication origins in escherichia coli cells can be measured after treating the cells with rifampicin together with the cell division inhibitor cephalexin. as opposed to wild type cells, certain mutants contain, with high frequency, a number of origins different from 2n, indicating that the mutants do not initiate dna replication at ... | 1991 | 1925010 |
| [role of alpha-1 and alpha-2-adrenoreceptors in achieving the compensatory response of the hemostasis system to plasmin]. | | 1988 | 2849566 |
| antiviral chemoprophylaxis for cytomegalovirus infection. | | 1988 | 2849602 |
| phorbol ester and phospholipase c-induced growth hormone secretion from pituitary somatotroph adenoma cells in culture: effects of somatostatin, bromocriptine, and pertussis toxin. | to clarify the role of the breakdown of phosphatidylinositol 4,5-bisphosphate (pip2) in gh secretion in human somatotrophs and the effects of inhibitors of gh secretion on this mechanism, we studied the effects of 12-tetradecanoylphorbol-13-acetate (tpa) and phospholipase c (plase c) on gh secretion and the interactions of somatostatin (srih), bromocriptine, and pertussis toxin (iap) with tpa or plase c, using human gh-secreting pituitary adenoma cells in culture. srih (10(-9)-10(-7) m) inhibite ... | 1987 | 2880863 |
| structure of bordetella pertussis peptidoglycan. | bordetella pertussis tohama phases i and iii were grown to the late-exponential phase in liquid medium containing [3h]diaminopimelic acid and treated by a hot (96 degrees c) sodium dodecyl sulfate extraction procedure. washed sodium dodecyl sulfate-insoluble residue from phases i and iii consisted of complexes containing protein (ca. 40%) and peptidoglycan (60%). subsequent treatment with proteinase k yielded purified peptidoglycan which contained n-acetylglucosamine, n-acetylmuramic acid, alani ... | 1987 | 2887547 |
| extracellular atp itself elicits superoxide generation in guinea pig peritoneal macrophages. | extracellular atp itself elicited the generation of superoxide (o2-) in guinea pig peritoneal macrophages associated with an increase in cytosolic calcium ([ca2+]i). the atp-induced o2- generation was completely inhibited by pretreatment with pertussis toxin (pt) accompanied by the suppression of [ca2+]i mobilization. pre-exposure to a small amount of phorbol myristate acetate (pma) primed the atp-induced generation of o2- without a change of [ca2+]i. the results suggest that atp-induced o2- gen ... | 1991 | 1851110 |
| intrathecal synthesis of antibodies to human t lymphotropic virus type i and the presence of igg oligoclonal bands in the cerebrospinal fluid of patients with endemic tropical spastic paraparesis. | tropical spastic paraparesis (tsp), a neuromyelopathy predominantly involving the pyramidal tract and commonly observed in tropical and equatorial areas, was recently found to be associated with human t lymphotropic virus type i (htlv-i). we investigated sera and cerebrospinal fluid (csf) from 19 patients with tsp who were from the caribbean area, french guiana, and africa. our results showed an elevated intra-blood-brain barrier igg synthesis rate and an elevated igg index, with an increased ht ... | 1988 | 2897399 |
| future trends in the management of peptic ulcer disease. | future trends in the short- and long-term management of peptic ulcer disease are considered. the present state of development of pharmacologic agents for the short-term healing of duodenal and gastric ulcer is impressive, and high rates of healing with rapid symptom relief can be safely achieved with several agents. the problem of ulcer recurrence has not been solved, although new work concerning the role of campylobacter pylori holds promise. the mortality of acute gastrointestinal haemorrhage ... | 1988 | 2906462 |
| protective role of serum antibody in immunity to chlamydial genital infection. | female guinea pigs were injected intraperitoneally with pooled immunoglobulin derived from animals immunized to the chlamydial agent of guinea pig inclusion conjunctivitis. genital infections in animals receiving pooled immunoglobulin from immune animals were markedly decreased with regard to the number of inclusions detected compared with control animals. these data indicated that serum-derived antibody was able to provide a degree of protection against a chlamydial genital tract infection. | 1989 | 2909492 |
| [adaptive effect and withdrawal syndrome of propranolol in relation to norepinephrine and epinephrine]. | | 1985 | 2934953 |
| influence of anticoagulants on growth and vitamin k production of intestinal bacteria. | the influence of anticoagulants on growth and vitamin k production of intestinal bacteria was studied. bacteroides vulgatus and escherichia coli were grown in vitro in the presence of increasing amounts of warfarin, phenprocoumon and acenocoumarol. it was found that growth of b. vulgatus was inhibited under anaerobic conditions whereas growth of e. coli under aerobic conditions was not inhibited. a specific inhibition of vitamin k biosynthesis was not observed in either case. it is concluded tha ... | 1991 | 1798715 |
| the effect of oral retinoid therapy on the normal human immune system. | twenty four patients were studied prior to and after 6 and 12 weeks therapy with isotretinoin (17 patients) for acne and related disorders, or with etretinate (7 patients) for psoriasis and related disorders. patients treated with isotretinoin had a significant reduction in natural killer cell activity at an effector: target cell ratio of 100: 1 at 12 weeks and also a reduction in natural killer cell numbers at this time. patients treated with etretinate had elevated natural killer cell activity ... | 1988 | 2972309 |
| oestrus phases of the syrian hamster. | | 1988 | 2976222 |
| restriction endonuclease mapping and cloning of mycobacterium fortuitum var. fortuitum plasmid pal5000. | a restriction map of mycobacterium fortuitum var. fortuitum plasmid pal5000 was established. the unique sites for apai, bamhi, bglii, bsteii, clai, ecori, ecorv, hpai, kpni and nari were located on the 5.0-kb plasmid. the plasmid had no sites for ahaiii, bcli, hindiii, psti, sphi and xbai. pal5000 was cloned into pbr322 and propagated in escherichia coli. three hybrid pal5000-pbr322 plasmids carrying the complete pal5000 sequence were constructed by joining the plasmids at their bamhi, ecori or ... | 1985 | 3002238 |
| restriction endonuclease mapping and cloning of mycobacterium fortuitum var. fortuitum plasmid pal5000. | a restriction map of mycobacterium fortuitum var. fortuitum plasmid pal5000 was established. the unique sites for apai, bamhi, bglii, bsteii, clai, ecori, ecorv, hpai, kpni and nari were located on the 5.0-kb plasmid. the plasmid had no sites for ahaiii, bcli, hindiii, psti, sphi and xbai. pal5000 was cloned into pbr322 and propagated in escherichia coli. three hybrid pal5000-pbr322 plasmids carrying the complete pal5000 sequence were constructed by joining the plasmids at their bamhi, ecori or ... | 1985 | 3002238 |
| demonstration of non-degraded aleutian disease virus (adv) proteins in lung tissue from experimentally infected mink kits. brief report. | isolates of adv replicate to rather high quantities in lungs from neonatally infected mink kits. the virus was analysed for polypeptide composition, and for the first time high molecular weight polypeptides have been observed in in vivo produced advs. these polypeptides are analogous to those of in vitro produced advs. the molecular weights of the structural polypeptides of the low virulence pullman adv and the highly virulent dk and utah i isolates of adv were found to be 88kd and 78kd and in v ... | 1986 | 3002299 |
| central nervous system lesions in suckling mice and rats inoculated intranasally with sialodacryoadenitis virus. | suckling cd-1 and cfw mice and wi wistar rats were inoculated intranasally with sialodacryoadenitis virus. in animals inoculated during the first week of life, there was an acute necrotizing encephalitis with malacia and minimal inflammatory cell response. in mice and rats which survived for up to 12 days post inoculation, loss of brain substance, gitter cells and occasionally mineralized debris were seen. viral antigen was readily demonstrated in neurons and nasal epithelium in trypsin-treated, ... | 1986 | 3004003 |
| [morphological changes caused by adrenalectomy in rabbits]. | | 1986 | 3019628 |
| [morphological changes caused by adrenalectomy in rabbits]. | | 1986 | 3019628 |
| [nuclear magnetic resonance spectroscopy in medicine: biochemistry of intact tissues]. | | 1986 | 3020443 |
| [nuclear magnetic resonance spectroscopy in medicine: biochemistry of intact tissues]. | | 1986 | 3020443 |
| formation of lattice-like inclusions in circulating erythroblasts of the laboratory mouse (hea/hea) with hereditary congenital erythroblastic anemia. | | 1986 | 3020854 |
| immunosuppressive properties of human placenta: study of supernatants from short-term syncytiotrophoblast cultures. | using collagenase and mechanical treatment to attempt to eliminate cellular contamination such as macrophages and decidual cells, trophoblast enriched cell suspensions were isolated from the human placenta. with a view to assessing the role of trophoblast in impairing maternal rejection of the fetus, supernatants (spl4) were prepared from these placental cells after short-term culture (4 h). the immunosuppressive activity of these supernatants was studied following application to mitogen-stimula ... | 1986 | 3023607 |
| the anti-tachycardic mechanism of a direct-acting vasodilator, budralazine, in rats. | the present study was undertaken to elucidate the anti-tachycardic effect of a direct-acting vasodilator, budralazine, using an electrophysiological technique. normotensive male wistar rats were used. rats were anesthetized intraperitoneally with urethane and alpha-chloralose. intravenous administration of budralazine (0.5-5.0 mg/kg) produced a dose-dependent reduction of mean arterial pressure in anesthetized rats. at doses of 0.5 and 1.0 mg/kg, budralazine induced bradycardia accompanied with ... | 1991 | 1773498 |
| localization of quantitative changes in pulmonary beta-receptors in ovalbumin-sensitized guinea pigs. | impaired beta-receptor function has been postulated as one factor contributing to airway hyperreactivity in asthmatic patients. although numerous indirect studies have cast doubt on this theory, none of these previous investigations has been able to directly measure changes in beta-receptor number on intrapulmonary structures capable of affecting the physiologic changes seen in this disease state. to help clarify the intrapulmonary location of such changes, a model of allergic bronchoconstrictio ... | 1987 | 3037954 |
| t and b peripheral blood lymphocytes in normal and lymphocytotic sheep. | surface immunoglobulins (sig), peanut agglutinin (pna), spontaneous erythrocyte rosette (e-rosette) and helix pomatia (hp) marker were investigated in normal and bovine leukemia virus (blv)-infected sheep. in normal sheep, 19.3% +/- 4.9 of peripheral blood lymphocytes (pbl) were sig+, whereas 58% +/- 5.69 were pna+, and 19.6 +/- 5.2 were e-rosette forming cells (e-rfc). in blv-induced lymphocytotic sheep, sig+ cells in pbl reached 59.4% +/- 15.06. in the same animals, pna bound to 20.6% +/- 9.69 ... | 1987 | 3040327 |
| roles of hormonal and nutritional factors in the regulation of rat liver alcohol dehydrogenase activity and ethanol elimination rate in vivo. | fasting reduced the liver alcohol dehydrogenase (adh) activity by 51% (p less than 0.001). insulin, within 2 hr, increased the adh activity found in fasted animals by 28% (p less than 0.02). insulin administration failed to stimulate the reduced adh activity in diabetic rats. however, adh activity in the diabetic-fed rats decreased by 52-54% (p less than 0.001) compared to normal-fed rats regardless of whether they were meal-fed or refed the normal chow. glucagon blocked by 15% (p less than 0.02 ... | 1988 | 3044171 |
| free insulin profiles during intensive treatment with biosynthetic human insulin. | 24 h profiles of free insulin and glucose were determined in insulin-dependent diabetic patients on intensive insulin regimens with biosynthetic human insulin, either as continuous subcutaneous insulin infusion (csii) with mealtime bolus doses (n = 6), or intensified conventional insulin therapy (icit) with preprandial injections of regular insulin and intermediate-acting insulin at bedtime (n = 6). the free insulin profiles were similar to the normal profiles but there were some important diffe ... | 1988 | 3044863 |
| cytoarchitecture, neuronal composition, and entorhinal afferents of the flying fox hippocampus. | in a comparative approach, the anatomical organization of the hippocampus was investigated in two species of megachiropteran bats, the grey-headed flying fox, pteropus poliocephalus, and the little red flying fox, pteropus scapulatus. in general, the cytoarchitectonic appearance of the flying fox hippocampus corresponded well with that of other mammals, revealing all major subdivisions. while the dentate fascia was trilaminated with a molecular layer, a granule cell layer, and a distinct polymor ... | 1991 | 1727000 |
| inflammatory bowel disease induced by combined bacterial immunization and oral carrageenan in guinea pigs. model development, histopathology, and effects of sulfasalazine. | a model of experimentally induced inflammatory bowel disease (ibd) featuring colitis, originally devised by onderdonk and co-workers in guinea pigs, was modified to establish the optimal conditions for ulcer development. upon varying the time of subcutaneous immunization with bacteroides vulgatus and concomitant oral administration of acid-degraded iota-carrageenan and viable b. vulgatus, it was found that the optimal times of administering these agents were one to two weeks and five to six days ... | 1991 | 1672517 |
| some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate. | lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ... | 1986 | 3085110 |
| some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate. | lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ... | 1986 | 3085110 |
| antimicrobial resistance in streptococcus pneumoniae: a south african perspective. | resistance to penicillin among south african strains of streptococcus pneumoniae increased from 4.9% in 1979 to 14.4% in 1990. except for resistance to co-trimoxazole (44%), resistance to other antimicrobial agents remained relatively low. multiply resistant strains belonged mainly to serovars 6b, 19a, 14, and, more recently, 23f. use of chloramphenicol to treat meningitis caused by strains relatively resistant to penicillin proved to be unsatisfactory, probably because of the inadequate bacteri ... | 1992 | 1617077 |
| [effect of electro-acupuncture on the cerebral volume of blood flow during experimental cerebral infarction]. | | 1986 | 3102119 |
| survey of bacteroides fragilis group susceptibility patterns in canada. | the in vitro activities of penicillin, clindamycin, chloramphenicol, metronidazole, piperacillin, piperacillin-tazobactam, ticarcillin, ticarcillin-clavulanate, ampicillin-sulbactam, cefoxitin, ceftizoxime, cefotetan, moxalactam, and imipenem against 348 bacteroides fragilis group isolates collected from six canadian cities during 1990 were determined by the national committee for clinical laboratory standards (nccls) agar dilution technique. all isolates were susceptible to chloramphenicol, met ... | 1992 | 1605600 |
| inducible macrolides-lincosamides-streptogramin b resistance in bacteroides species. | the first evidence is presented for the presence of inducible macrolides-lincosamides-streptogramin b resistance in bacteroides species. different macrolides induced clindamycin resistance in bacteroides vulgatus ryc18f6, an erythromycin-resistant and clindamycin-susceptible strain. a study of 144 bacteroides isolates indicated that erythromycin resistance was linked to diminished clindamycin susceptibility. | 1987 | 3111358 |
| [feed-dependent changes in the rumen mucosa of high-producing cows from the dry period to eight weeks post partum]. | | 1987 | 3122467 |
| role of intestinal anaerobic bacteria in colonization resistance. | the purpose of this study was to clarify the role of the intestinal anaerobe bacteria in colonization resistance. germfree mice were associated with escherichia coli c25 and either (a) no other species; (b) enterococcus; (c) escherichia coli m14 and proteus mirabilis, or (d) bacteroides fragilis and bacteroides vulgatus. all species colonized the cecum in high numbers, but only enterococcus significantly limited the translocation of escherichia coli c25 to mesenteric lymph nodes. however, the ov ... | 1988 | 3132368 |
| intravenous immune globulin in symptomatic paediatric human immunodeficiency virus infection. | seven paediatric patients with symptomatic human immunodeficiency virus (hiv) infection were prospectively studied for 6-24 months after the start of intravenous immune globulin (ivig) therapy. there was substantial clinical benefit during ivig treatment with marked reduction of febrile and infectious episodes, normalization of physical and psychomotor development, and absence of mortality. the immunologic monitoring revealed some discrete objective improvements. the results of this study favour ... | 1988 | 3134244 |
| distribution of sensory receptors in the flexor carpi radialis muscle of the cat. | the structures and distribution of encapsulated muscle receptors were examined in serial transverse sections of flexor carpi radialis in the adult cat. four types of receptors (muscle spindles, golgi tendon organs, paciniform, and pacinian corpuscles) were identified. their structures resembled those encountered in other limb muscles. pacinian corpuscles were rare and occurred only in the external fascial coat of the muscle near its origin. the other three receptor types were distributed in an u ... | 1985 | 3156250 |
| non-immune igg f(ab')2 binding to group c and g streptococci is mediated by structures on gamma chains. | the present investigation was designed to determine whether the heavy or the light immunoglobulin chain is involved in the non-immune binding of igg f(ab')2 fragments to specific surface receptors on human group c and g streptococci. purified human polyclonal igg was mildly reduced with dithiothreitol and alkylated with iodoacetamide. light (l) and heavy (h) chains were separated. intact igg and purified l and h chains of polyclonal immunoglobulin g were tested in an inhibition assay for non-imm ... | 1985 | 3156400 |
| mapping the biochemical trajectory of nephrotoxicity by pattern recognition of nmr urinanalysis. | | 1992 | 1489674 |
| sulphasalazine fails to prevent development of mucosal ulceration and 5-lipoxygenase activity in guinea-pigs with chronic inflammatory bowel disease induced by combined bacterial immunization and oral carrageenin. | a model of inflammatory bowel disease in guinea-pigs involving a 14 day initial treatment with formalin-killed bacteroides vulgatus subcutaneously and oral carrageenin plus live b. vulgatus for 10 days was used to determine the effects of sulphasalazine 100 mg kg-1 day-1 b.i.d., p.o. given for 4, 7 and 10 days after cessation of the bacterial/carrageenin treatment on the morphological and histological states of the established disease and on the production of the principal 5-lipoxygenase product ... | 1992 | 1359081 |
| german multicentre study on the in vitro susceptibility of bacteroides species. the german bacteroides study group. | in 1990 the first german multicentre study on the in vitro susceptibility of bacteroides species was completed. employing a commercially prepared microbroth dilution assay, nine participating institutions evaluated approximately 100 consecutive isolates of bacteroides species from relevant clinical specimens. a total of 911 strains (449 bacteroides fragilis, 201 bacteroides thetaiotaomicron, 79 bacteroides ovatus, 78 bacteroides vulgatus, 77 bacteroides distasonis, 25 bacteroides uniformis, 2 ot ... | 1992 | 1295762 |
| incidence and pathological features of spontaneous tumors in athymic nude mice. | systematic observation of 1141 nude mice (swiss background [corrected] strain) that received human tumor transplants revealed 24 spontaneous tumors, 18 of lymphoreticular origin and 6 pulmonary adenomas. spontaneous tumors were seen at an average age of 9.1 months, and 22 of the tumors were seen only in that fraction of our group (324 mice) surviving for 5 months or more (22 of 324 x 100 = 6.8%). transplantation of these tumors to other nude mice was successful in three of five cases. mice trans ... | 1979 | 427772 |
| comparison of primary and secondary stimulation of male rats by estradiol in terms of prolactin synthesis and mrna accumulation in the pituitary. | male rats received acute or chronic primary or acute secondary stimulation with estradiol, and the effects on pituitary prolactin synthesis and its mrna accumulation were examined. prolactin synthesis was determined by the in vitro incorporation of [(3)h]leucine into prolactin over a period of 1 hr. prolactin mrna was measured both by cell-free translation in a nuclease-treated rabbit reticulocyte lysate and by hybridization to the complementary dna. the latter two methods gave similar results u ... | 1979 | 284404 |
| trace polypeptides in cellular extracts and human body fluids detected by two-dimensional electrophoresis and a highly sensitive silver stain. | development of a highly sensitive silver stain permits the characterization of trace cellular and body fluid proteins separated by the two-dimensional electrophoresis technique of o'farrell. many of the proteins detected by the silver stain in urine, spinal fluid, amniotic fluid, and cells were undetected with the widely used coomassie blue stain. trace polypeptides observed in escherichia coli cell lysates with this silver stain could be detected previously only by growth in radioactive precurs ... | 1979 | 92027 |
| the use of trypan blue for counterstaining in the sepharose bead immunofluorescence test. the application of the test for the demonstration of primate retrovirus-specific antibodies and antigens. | the sepharose bead immunoflurorescence test was performed by counterstaining the beads with trypan blue. this results in a red staining of negative beads which allows an easy distinction from positive green-fluorescent beads. sepharose beads conjugated with viral proteins or antiviral antibodies were used to demonstrate mason-pfizer monkey virus (mpmw)- and simian sarcoma virus (ssv) - specific antigens or antibodies. the test shows a high sensitivity and specificity and needs a small amount of ... | 1978 | 86253 |
| evaluation of the syphilis bio-enzabead assay for detection of treponemal antibody. | an enzyme-linked immunosorbent assay for treponema pallidum antibody (syphilis bio-enzabead kit; organon teknika corp., formerly litton bionetics, kensington, md.) was compared with the standard fluorescent treponemal antibody absorption test for syphilis (indirect fluorescent-antibody confirmatory test; zeus scientific inc., raritan, n.j.). six hundred specimens tested in the rapid plasma reagin card test (hynson, westcott and dunning, inc., baltimore, md.) and microhemagglutination assay for a ... | 1987 | 3294884 |
| menaquinone production and utilization in germ-free rats after inoculation with specific organisms. | although menaquinones are biologically active forms of vitamin k, factors that influence their production by bacteria or their absorption from the gut are not well understood. germ-free rats were inoculated with four different strains of organisms and fecal and tissue menaquinone concentrations were determined. no menaquinones were detected in the tissues or feces of rats colonized with bifidobacterium longum or clostridium ramosum, two organisms that have not been reported to produce menaquinon ... | 1987 | 3298580 |
| deconjugation of bile acids by human intestinal bacteria implanted in germ-free rats. | fecal bile acids in germ-free rats were analyzed after inoculation with bacteroides vulgatus, bifidobacterium longum, escherichia coli or clostridium ramosum. b. vulgatus preferentially deconjugated tauro-beta-muricholic acid and b. longum taurocholic acid. c. ramosum deconjugated both bile acids, but e. coli deconjugated neither. 7 alpha-dehydroxylation of bile acids was negligible even after 18 days of inoculation, but a small amount of 7-oxo-bile acids, less than 5%, was formed. fecal excreti ... | 1987 | 3312906 |
| [medico-genetic studies of human provisional organs]. | | 1987 | 3318529 |
| recurrent hypercalcemia and elevated 1,25-dihydroxyvitamin d levels in hodgkin's disease. | hypercalcemia has been infrequently associated with hodgkin's disease. when seen, most cases have been attributable to skeletal invasion by disease. herein is described a 40-year-old man with a 15-year history of hodgkin's disease. each of four disease recurrences was heralded by hypercalcemia occurring in the absence of bone disease or elevation of parathyroid hormone levels. marked elevations of 1,25-dihydroxyvitamin d levels were observed that paralleled his disease course and response to the ... | 1988 | 3337120 |
| the cpg dinucleotide and human genetic disease. | reports of single base-pair mutations within gene coding regions causing human genetic disease were collated. thirty-five per cent of mutations were found to have occurred within cpg dinucleotides. over 90% of these mutations were c----t or g----a transitions, which thus occur within coding regions at a frequency 42-fold higher than that predicted from random mutations. these findings are consistent with methylation-induced deamination of 5-methyl cytosine and suggest that methylation of dna wit ... | 1988 | 3338800 |
| rate of incorporation of radiolabelled nucleosides does not necessarily reflect the metabolic state of cells in culture: effects of latent mycoplasma contamination. | in response to cell-free conditioned medium derived from the human bladder carcinoma line t24 (t24 sn), we found greatly reduced incorporation of tritiated thymidine and uridine ([3h]tdr, [3h]ur) by the human carcinoma lines uchncu (small-cell lung carcinoma) and ls174t (colon carcinoma). the effect was not due to an excess of nucleosides or cytokines known to be present in t24 sn. cell-cycle distribution, increase in cell numbers, and de novo nucleoside synthesis in the indicator cells were onl ... | 1988 | 3338817 |
| formation of dna adducts and water-soluble metabolites of benzo[a]pyrene in human monocytes is genetically controlled. | formation of dna adducts and of water-soluble metabolites was studied in monocytes of 86 first-degree relatives of 15 families. tests were performed with blood monocytes using (g-3h)-benzo[a]pyrene as a model pro-carcinogen. variance analysis revealed significantly higher inter-familial than intra-familial variations. from these data we conclude that the formation of dna adducts is genetically controlled. therefore the enhanced formation of benzo[a]pyrene dna adducts in lung cancer patients foun ... | 1988 | 3338869 |
| chromosome analysis of human oocytes and embryos: does delayed fertilization increase chromosome imbalance? | thirty per cent of a sample of 120 unfertilized human oocytes carried chromosome abnormalities highly correlated with maternal age (38% in patients greater than 35, as compared with 24% in younger patients). fertilized eggs, when observed 17 h after insemination, showed in 1.6% a single pronucleus suggesting parthenogenetic activation. in 92% of the cases two pronuclei were observed and the rate of chromosome anomalies depended on the morphological aspect of the embryos. triploidy was also encou ... | 1988 | 3350934 |
| occurrence of two phases of return to oestrus in sows on commercial units. | | 1988 | 3376405 |
| bacteroides vulgatus outer membrane antigens associated with carrageenan-induced colitis in guinea pigs. | previous experiments with the carrageenan model for ulcerative colitis demonstrated that the inflammatory response in guinea pigs can be enhanced by immunization with bacteroides vulgatus and subsequent feeding of this organism to experimental animals. the studies reported here show that antigens extractable from the bacterial outer membrane by edta are responsible for this effect. sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to analyze the outer membrane proteins from vari ... | 1988 | 3384476 |
| purification and characterization of a new hydrolase for conjugated bile acids, chenodeoxycholyltaurine hydrolase, from bacteroides vulgatus. | a new hydrolase for conjugated bile acids, tentatively named chenodeoxycholyltaurine hydrolase, was purified to homogeneity from bacteroides vulgatus. this enzyme hydrolyzed taurine-conjugated bile acids but showed no activity toward glycine conjugates. among the taurine conjugates, taurochenodeoxycholic acid was most effectively hydrolyzed, tauro-beta-muricholic and ursodeoxycholic acids were moderately well hydrolyzed, and cholic and 7 beta-cholic acids were hardly hydrolyzed, suggesting that ... | 1989 | 2628421 |
| role of amino acids in modulating glucose-induced desensitization of the glucose transport system. | amino acids were found to play an integral role in modulating glucose-induced desensitization of the glucose transport system (gts). when adipocytes were treated for 6 h in a defined buffer containing 25 ng/ml insulin, 20 mm glucose, plus the 15 amino acids found in dulbecco's modified eagle's medium, we observed marked desensitization of the gts, manifested by a 60% decrease in maximal insulin responsiveness (mir) and a 2.5-fold reduction in insulin sensitivity. in contrast, little or no desens ... | 1989 | 2687266 |
| the effect of tissue culture agar on chromosome breakage, sister-chromatid exchanges and clonogenicity in human cells. | to investigate the cytogenetic effects of electromagnetic fields, a system containing an agar gel was developed to support the growth of various human cell types (peripheral lymphocytes, lymphoblasts, and fibroblasts). when compared to alioquots of identical cells, grown in plastic culture vessels, statistically significant increases in the frequencies of chromosome breakage, sister-chromatid exchange and decreased cloning efficiency were observed in those cells cultured in the agar. these resul ... | 1988 | 3398870 |
| high-performance liquid chromatography with on-line radioactivity monitoring for metabolic studies of 2- and 4-[14c]aminobiphenyls. | | 1988 | 3417828 |
| experimental evaluation of the mitroflow pericardial heart valve prosthesis. part ii. pathologic examination. | the results of the morphologic and histopathologic examination of 37 bovine pericardial heart valve prostheses that were retrieved after experimental implantation in chacma baboons for periods of one to twelve months are presented in this study. the implanted prostheses consisted of 33 mitroflow valves, size 21 mm, that belonged to four different groups according to the method of preparation of the pericardium (process i to iv) and 4 commercially available ionescu-shiley valves, size 19 mm. all ... | 1988 | 3421507 |
| effects of cysteine and antioxidants on the hepatic redox-state, acetaldehyde and triglyceride levels after acute ethanol dosing. | cysteine and the synthetic antioxidants butylated hydroxytoluene (bht) and n,n'-diphenyl-phenylenediamine (dppd) have been found to protect against the increase in hepatic triglycerides caused by acute ethanol administration (2 g/kg/i.p.) in rats. none of these agents affected the ethanol-induced increase in the hepatic redox-state, measured as lactate/pyruvate and 3-hydroxybutyrate/acetoacetate ratios, and there was no influence of any of the compounds on ethanol metabolism. of the three agents ... | 1987 | 3426693 |
| electrophysiological and behavioral changes after destruction of the basolateral amygdaloid nucleus in rats. | | 1987 | 3431682 |
| design, expression, and crystallization of recombinant lectin from the garden pea (pisum sativum). | the propeptide form of the lectin from the garden pea (pisum sativum agglutinin) has been expressed in escherichia coli by attaching its cdna to an inducible promoter. by a number of criteria, including the ability to form dimers, hemagglutination titer, western blot, and enzyme-linked immunosorbent assay, the resulting propeptide molecule is virtually indistinguishable from the mature proteolytically processed lectin isolated from peas. preliminary crystallization experiments using the recombin ... | 1989 | 2708344 |
| interactions between polymorphonuclear leucocytes, bacteroides sp, and escherichia coli: their role in the pathogenesis of mixed infection. | five bacteroides fragilis strains and five bacteroides vulgatus strains were compared with regard to their ability to consume complement and to fix c3, their killing by polymorphonuclear leucocytes, and their ability to inhibit the bactericidal effect of serum and polymorphs on escherichia coli strains. complement consumption was positively related to c3 fixation, but no relation was observed between these variables and the killing of the anaerobes. greatest inhibition of the killing of e coli b ... | 1986 | 3517072 |
| calcium: limited indications, some danger. | calcium chloride has been advocated since the 1920s for the resuscitation of asystole, electromechanical dissociation (emd), and ventricular fibrillation. reports of side effects and complications have been numerous. studies of calcium assays following american heart association recommended dosages have shown dangerously elevated serum levels. large retrospective clinical studies in milwaukee and tampa have found no evidence of improved survival with calcium chloride in asystole and emd. a prosp ... | 1986 | 3536166 |
| use of the glucose oxidase/peroxidase method for glucose assay leads to overestimation of the inhibition of gluconeogenesis by aminopyrine. | 4-aminoantipyrine strongly inhibits glucose determination by the glucose oxidase/peroxidase/dianisidine assay but does not interfere in the assay using glucose-6-phosphate dehydrogenase, hexokinase and atp. as a result, the inhibition of gluconeogenesis by aminopyrine reported to be 50-90% (bánhegyi, g., mandl, j., antoni, f. and garzó, t. (1987) biochim. biophys. acta 927, 406-416) is strongly overestimated and amounts to only 10-30%. | 1989 | 2713417 |
| transferable 5-nitroimidazole resistance in the bacteroides fragilis group. | we report the characterization of a strain of bacteroides vulgatus, bv17, that exhibits a moderate resistance to 5-nitroimidazoles and carries plasmids of 4.5, 5, 7.7, and 56 kb. a genetic determinant involved in this resistance is carried by the 7.7 +/- 0.2-kb plasmid (pip417). this plasmid can be introduced and replicated in a sensitive strain of b. fragilis 638r by transformation or by conjugation. in the latter case, the transfer may involve mobilization by the 56-kb conjugative plasmid (pip ... | 1989 | 2740453 |
| identification in rat liver and serum of water-soluble class i mhc molecules possibly homologous to the murine q10 gene product. | we have identified large quantities of a water-soluble, non-rt1.a class i mhc molecule in the serum of the da rat strain, with a similar molecule being found in aqueous extracts of da liver. the non-rt1.a class i molecules have heavy chains of 41 kd, which is smaller than rt1.a class i molecules isolated from liver membranes (45 kd) but larger than water-soluble rt1.a class i molecules previously identified in serum and aqueous extracts of liver and kidney (40 kd). nh3-terminal amino acid sequen ... | 1987 | 3585249 |
| increased faecal glycosidases in patients with crohn's disease. | the strictly anaerobic faecal flora of patients with crohn's disease (cd) contains higher numbers of coccoid rods (eubacterium, peptostreptococcus and coprococcus species) and gram-negative rods (bacteroides vulgatus) than the flora of healthy subjects. the abnormal flora of patients with cd might affect levels of glycolytic enzymes and metabolic products and thus play some role in the pathogenesis of cd. therefore, the activity of 23 glycosidases that can degrade mucus glycoproteins or plant po ... | 1987 | 3609504 |
| [mechanism of changes in irradiated thymocytes detected by anilinonaphthalenesulfonate]. | in experiments on rat thymocytes obtained 15 min, 2 h and 4 h after irradiation of animals with the dose of 10 gy it was found that 15 min and 4 h following irradiation changes occurred in their membranes increasing the lifetime of the excited state of anilinonaphthalene sulfonate and hydrofobicity and viscosity of its microenvironment. the indicated parameters did not vary from the controls 2 h following irradiation. the analysis of the data obtained prompted a suggestion that the observed chan ... | 1987 | 3628737 |
| [ultrastructure of human adrenal glands at various periods of prenatal morphogenesis]. | as demonstrates the electron microscopical investigation of the adrenals in human embryos and fetuses 6--32-week-old, at the age of 10--12 weeks glomerular and fasciculo-reticular zones are already differentiating. subsequently, during following stages of the prenatal morphogenesis, when ultramicroscopical signs of the zonal belonging of cells and specific activity of steroid-synthesizing organells, the cortical reaction to the birth stress is manifested. installation of the cells of the medulla ... | 1989 | 2764742 |
| micropuncture localization of the natriuretic effect of interleukin 1. | interleukin 1 (il-1) has been demonstrated to elicit an increase in renal sodium excretion. this effect occurs in the absence of any increase in the filtered load of sodium, raising the possibility of an il-1-mediated decrease in tubule sodium reabsorption. to localize the nephron segment(s) responsible for the natriuretic effect of il-1, we performed micropuncture experiments on rats. intravenous il-1 administration caused a marked increase in sodium excretion that was not accompanied by change ... | 1989 | 2785767 |
| prevalence of hepatitis b markers in occupational health nurses. | a seroprevalence study was undertaken at the 14th annual conference of the occupational health nurses association of ontario. a total of 151 self-selected registrants (33%) provided demographic, health, and occupational information. blood was drawn for hepatitis b serology (hepatitis b surface antigen, core antibody, and surface antibody). vaccine-induced seropositivity was found in 11 persons (7.3%) while naturally occurring markers were present in 13 nurses (8.6%). this exceeds the natural pre ... | 1987 | 3655952 |
| dual-column immunoassays using protein g affinity chromatography. | tandem protein g affinity and reversed-phase chromatography (rpc) columns, coupled with a switching valve, were used for on-line immunoassays of antibodies and antigens. columns with reversibly immobilized antibodies were prepared by adsorbing antibodies on the protein g column. following antigen capture in the immunoaffinity column, antigen-antibody complexes were desorbed, dissociated, and transferred to the rpc column where they were separated and quantified. this system was used to determine ... | 1989 | 2802148 |
| value of thyroid follow-up registers. | in this study from a county in southern sweden 269 of 271 patients (99.3%) treated with radioiodine for hyperthyroidism over a two-year period were followed up five years later. the routine procedures at hospital clinics and primary health care centres were studied. a high number of toxic multinodular goitre (n = 113; 42.0%) and toxic adenoma (n = 76; 28.3%) emerged, 80 patients (29.7%) had toxic diffuse goitre (graves's disease). during the five-year follow-up the cumulative incidence of hypoth ... | 1987 | 3671919 |
| the influence of various bacteria on the cecal mucosa of monoflora chickens infected with eimeria tenella. a scanning electron microscopic study. | monoflora chickens were established at the age of 2 days by an oral inoculation of one of six species of bacteria (lactobacillus acidophilus, bifidobacterium thermophilum, bacteroides vulgatus, clostridium perfringens, escherichia coli, or streptococcus faecalis). two days later the chickens were infected with eimeria tenella (5 x 10(4) oocytes per bird). there were four groups: uninfected birds as controls, the birds infected with either bacteria or e. tenella alone, and the birds infected with ... | 1987 | 3673342 |
| the binding of decomposition products of udp-galactose to the microsomes and polyribosomes isolated from rat liver. | udp-d-[u-14c]galactose is decomposed to [u-14c]galactose-1-phosphate and [u-14c]galactose by rat liver microsomal and crude polyribosomal fractions, under conditions commonly used to assay of glycosyltransferase activities. udp-d-[u-14c]galactose, at neutral ph, is also chemically degraded to the [u-14c]galactose-1,2-cyclic phosphate. the 1,2-cyclic phosphate derivative of galactose also exists in the commercial udp-d-[u-14c]galactose. it is a very important finding that products of the udp-d-[u ... | 1987 | 3675917 |
| an in vitro method for estimating biologically available vitamin b6 in processed foods. | 1. an in vitro method which used enzymic digestion of the food matrix to release biologically available vitamin b6 is described. 2. vitamin b6-fortified liquid model foods were thermally processed. after these foods had been freeze-dried, one part was subjected to enzymic hydrolysis at ph 2.0 with pepsin (ec 3.4.23.1) followed by a hydrolysis at ph 8.0 with pancreatin. the vitamins that were found in the supernatant fraction, after an acidified methanol treatment of the hydrolysate, were estimat ... | 1986 | 3676156 |
| reciprocal functional interaction of adjacent myocardial segments during regional ischemia: an intraventricular loading phenomenon affecting apparent regional contractile function in the intact heart. | the mechanism of the increase in function in myocardial segments adjacent to those subjected to regional ischemia is not known. to investigate the hypothesis that changes in regional loading are an important causative factor of this phenomenon, 17 dogs were instrumented with segmental shortening sonomicrometers in the left anterior descending and circumflex artery distributions, circumflex coronary flow probes and cuff occluders, as well as circumflex coronary pressure catheters. additionally, t ... | 1986 | 3711491 |
| reciprocal functional interaction of adjacent myocardial segments during regional ischemia: an intraventricular loading phenomenon affecting apparent regional contractile function in the intact heart. | the mechanism of the increase in function in myocardial segments adjacent to those subjected to regional ischemia is not known. to investigate the hypothesis that changes in regional loading are an important causative factor of this phenomenon, 17 dogs were instrumented with segmental shortening sonomicrometers in the left anterior descending and circumflex artery distributions, circumflex coronary flow probes and cuff occluders, as well as circumflex coronary pressure catheters. additionally, t ... | 1986 | 3711491 |
| bvd virus infection and embryo transfer. | | 1986 | 3716157 |
| properties of the penicillin-binding proteins of four species of the genus bacteroides. | the penicillin-binding proteins (pbps) of four species of the genus bacteroides were examined in cell envelope preparations from exponentially growing cultures and intact cells. upon examination by sodium dodecyl sulfate-polyacrylamide electrophoresis, three major high-molecular-weight pbps (molecular weight, 58,000 to 82,000) were resolved, and low-molecular-weight pbps were seen in all strains except bacteroides fragilis. the sporadic appearance of pbp 4 in b. fragilis (molecular weight, appro ... | 1986 | 3729342 |
| evidence that polygalacturonic acid may not be a major source of carbon and energy for some colonic bacteroides species. | five bacteroides species that are found in the human colon can utilize polygalacturonic acid (pga) when they are grown in laboratory media: bacteroides thetaiotaomicron, bacteroides vulgatus, bacteroides ovatus, bacteroides fragilis subsp. a, and bacteroides sp. strain 3452a (an unnamed dna-dna homology group). pga-degrading enzymes from b. thetaiotaomicron have been isolated and characterized previously. to determine whether a pga lyase activity in human feces could be attributed to any of thes ... | 1986 | 3729407 |