| [isolation of rickettsia of the spotted fever group (sf group) from dermacentor marginatus ticks in tirol, austria]. | | 1977 | 410728 |
| in vitro guinea pig leukocyte reactions to rickettsia rickettsii. | the presence of cell-mediated immunity in rocky mountain spotted fever-infected guinea pigs was determined by two in vitro assays: whole blood lymphocyte transformation (lt) and macrophage migration inhibition. increased lt was detected as early as 1 week in guinea pigs infected with rickettsia rickettsii and treated with oxytetracycline and was detected by two weeks in infected but untreated guinea pigs. elevated lt was still detectable at 10 weeks postinfection. guinea pigs vaccinated with kil ... | 1977 | 412792 |
| [comparative precipitation studies between the highly-virulent virus of european swine fever, swine-fever-virus split products and precipitating swine-fever antigens from the organs of swine-fever infected pigs]. | | 1977 | 414484 |
| [auricular fibrillation with ventricular tachycardia. surgical section of a left lateral bundle of kent]. | the authors report the case of a man of 22 years, with no previous cardiac history, in whom a wolff-parkinson-white syndrome was discovered during the course of this first episode of atrial fibrillation with a ventricular rate of 300-350/mn; investigation (vecto-cardiogram, intra-cavitory ecg) showed the presence of a short circuit of the kent type, with a high permeability, and localised in the left lateral position; this was confirmed by pericardial cartography. after the surgical division of ... | 1977 | 414674 |
| complement in cystic fibrosis. | complement components c3, c4, and c3a were estimated in 30 patients with cystic fibrosis aged 1 to 21 years (m:f = 16:14) and were compared with results in 40 healthy, age-matched subjects. the influences of the clinical score, sputum microbiology, and the patients' sex were also investigated. in contrast to most previous communications, this paper shows that, compared to the control group, a significant decrease of c3 (p less than 0.001) and c4 (p less than 0.02) was observed whereas c3a levels ... | 1978 | 414916 |
| comparative electrophoresis of spotted fever group rickettsial proteins. | | 1978 | 415197 |
| listeria monocytogenes meningitis: case report. | a case of listeria monocytogenes septicaemia with meningitis is described. predisposing factors in this case included chronic liver disease, diabetes mellitus and liver malignancy. the infection responded poorly to ampicillin. | 1977 | 415268 |
| [cellular composition of the lymph nodes of monkeys (rhesus macaque) under normal and experimental conditions]. | by means of mathematical methods, quantitative and qualitative changes were studied in different structural components of the mesenteric (ileocecal) lymph nodes in normal monkeys (macaca rhesus) and under per os administration of salmonella typhi murium, streptomycin-dependent. cellular composition was calculated in the cortical plateau, cortical (lymphoid) cords and in follicules. average percent of every cell type was determined. vaccine administration, was stated to inhibit cytopoiesis in the ... | 1978 | 415703 |
| rocky mountain spotted fever caused by blood transfusion. | transfusion of 500 ml of blood, contributed by a donor three days before the onset of rocky mountain spotted fever and refrigerated for nine days, caused this disease in the recipient. the blood donor died of rocky mountain spotted fever after six days; rickettsia were identified in various tissues by immunofluorescence techniques. the recipient of the blood became mildly ill and recovered fully; specific antibiotic treatment was initiated on the fourth day of illness. diagnosis of rocky mountai ... | 1978 | 418193 |
| synthesis of plasma membrane proteins during development of dictyostelium discoideum. | | 1979 | 421897 |
| acute renal failure in rocky mountain spotted fever. | review of clinical and pathologic data from ten fatal cases of rocky mountain spotted fever (rmsf) revealed the importance of acute renal failure in the clinical course and of multifocal perivascular interstitial nephritis as the principal pathologic lesion. in nine cases, rickettsia rickettsii were demonstrated by immunofluorescence in the areas of vasculitis. evidence was lacking for the role of disseminated intravascular coagulation, glomerulonephritis, or myoglobinuria in the pathogenesis of ... | 1979 | 434998 |
| the efficacy of a potassium-rich diet compared to diuretic treatment on the renal elimination of thallium in the rat. | | 1979 | 454183 |
| comparison of individual and cumulative dose-response curves [proceedings]. | | 1979 | 497601 |
| kidney lesions in rocky mountain spotted fever: a light-, immunofluorescence-, and electron-microscopic study. | the essential pathologic lesion in rocky mountain spotted fever (rmsf) is a vasculitis that may involve the kidneys as well as the heart, brain, skin, and subcutaneous tissues. histopathologic information concerning the response of the kidneys in rmsf is rather limited, however. in this study renal tissue from 17 children who died of rmsf was examined by light, electron, and immunofluorescence microscopy. a lymphocytic or mixed inflammation, or both, involving vessels and interstitium of the kid ... | 1979 | 525676 |
| myocardial involvement in rocky mountain spotted fever. | rocky mountain spotted fever (rmsf), an acute febrile exanthematous illness caused by rickettsia rickettsii and transmitted by ticks, is endemic in the southern atlantic states. this report is based on the clinical and pathological findings of myocardial involvement in 16 children who died with severe rmsf. all 16 children had myocardial lesions to some degree, but it was not believed that these could be evaluated in terms of cardiac function and death in the face of the usual peripheral vascula ... | 1978 | 580868 |
| immunological properties of rickettsia rickettsii purified by zonal centrifugation. | the properties of rickettsia rickettsii purified from infected chicken yolk sacs or mouse l cell cultures by sucrose density gradient centrifugation in a zonal rotor were examined in various ways. rickettsiae derived from both l cells and yolk sacs had similar compositions: about 12% nitrogen, 1.5% phosphorus, 5% carbohydrate, and 30% fatty acids. on a dry-weight basis, purified rickettsiae were at least 2,000 times as effective as a commercial spotted fever vaccine in protecting guinea pigs aga ... | 1975 | 806522 |
| rhipicephalus sanguineus: vector of a new spotted fever group rickettsia in the united states. | a rickettsia related to but distinct from the spotted fever agent, rickettsia rickettsii, has been detected in 167 (18.9%) of 884 rhipicephalus sanguineus taken off dogs in central and northern mississippi. the organisms could readily be isolated in male meadow voles (microtus pennsylvanicus), where it produced massive infections in the tissues of tunica vaginalis. it was practically nonpathogenic for male guinea pigs, although inoculation of these animals with infected tunica vaginalis of voles ... | 1975 | 806533 |
| preparation of rocky mountain spotted fever vaccine suitable for human immunization. | rocky mountain spotted fever vaccine was produced from rickettsiae grown in chicken embryo cells in roller bottle cultures. the rickettsiae were concentrated and purified by passage through a sucrose gradient and inactivated with formalin. this vaccine satisfactorily passed preinactivation and final container testing and is believed to be superior to the presently available yolk sac vaccine. | 1975 | 809483 |
| comparison of three rocky mountain spotted fever vaccines. | growth of rocky mountain spotted fever (rmsf) rickettsiae in duck embryo cell (dec) cultures and chicken embryo cell (cec) cultures was evaluated. experimental lots of duck embryo cell- and chicken embryo cell-grown rocky mountain spotted fever vaccines and a commercial lot of yolk sac-grown vaccine were compared for protective efficacy in rhesus monkeys. incidence and magnitude of antibody response, febrile response, and rickettsemia, as well as incidence of fatalities, suggested that both cell ... | 1975 | 810494 |
| a review of rocky mountain spotted fever (tick-borne typhus), its agent, and its tick vectors in the united states. | | 1975 | 810584 |
| rocky mountain spotted fever (tick-borne typhus) in south carolina: an educational program and tick/rickettsial survey in 1973 and 1974. | because the incidence of spotted fever is increasing in south carolina, campaigns were carried out in 1973 and 1974 to provide the public and medical practitioners, through pamphlets and news media, with information about spotted fever and the ticks which transmit the causative agent, rickettsia rickettsii. people were also invited to save and submit live ticks removed from vegetation, animals and humans, for examination by the hemolymph test. a total of 1,186 ticks consisting of 987 dermacentor ... | 1975 | 811127 |
| indirect hemagglutination test for human antibody to typhus and spotted fever group rickettsiae. | an indirect hemagglutination (iha) test is described that uses glutaraldehyde-stabilized erythrocytes treated with a rickettsial erythrocyte-sensitizing substance obtained from rickettsia typhi or rickettsia rickettsii. the serological reagent was stable for at least 3 months at room temperature and 6 months at 4 c. it exhibited group specificity and no group cross-reactivity. at a minimum dilution of 1:40, acute and early convalescent epidemic and murine typhus antisera showed 86% positive reac ... | 1975 | 811685 |
| evaluation ofprimary blood monocyte and bone marrow cell culture for the isolation of rickettsia rickettsii. | rickettsia rickettsii was isolated from experimentally infected guinea pigs by culture of blood monocytes and bone marrow cells, and from experimentally infected rhesus monkeys by blood monocyte culture. rickettsiae were identified in monocyte-macrophage monolayers stained by giménez or flourescent antibody techniques. a total of 78 culture attempts were made from 20 guinea pigs and 16 monkeys. the success of isolation of r. rickettsii in culture was positively correlated with the numbers of ric ... | 1975 | 812828 |
| early diagnosis of rocky mountain spotted fever. use of primary monocyte culture technique. | diagnosis of rocky mountain spotted fever was made as early as the fourth day of febrile illness in rhesus monkeys (macaca mulatta), with a monocyte culture technique that is easily amenable to use in humans. rickettsia rickettsii were identified by direct immunofluorescent and gimenez staining of cultures of circulating monocytes from four experimentally infected monkeys, even in the presence of a mild or inapparent disease in three. levels of rickettsemia were estimated by the plaque-assay tec ... | 1976 | 814256 |
| rocky mountain spotted fever in mississippi: survey for spotted fever antibodies in dogs and for spotted fever group reckettsiae in dog ticks. | during epidemiologic studies of rocky mountain spotted fever in mississippi in 1973, dogs were surveyed for antibodies tp spotted fever group antigens and for tick parasites infected with rickettsiae of the spotted fever group. fifty-three (46%) of 116 serum samples had complement-fixing antibody titers greater than or equal to 1:8 as compared to only one (5%) of 21 samples from a group of dogs obtained from metropolitan chicage. fifty-two per cent of the dogs tested from mississippi had microag ... | 1976 | 814809 |
| microimmunofluorescence test for the serological study of rocky mountain spotted fever and typhus. | a microimmunofluorescence test was used to study antibody responses to various spotted fever group and typhus group rickettsiae during rocky mountain spotted fever (rmsf) and epidemic typhus (et). patients with rmsf reacted most strongly to rickettsia rickettsii; those with et reacted predominantly to r. prowazekii. the degree of cross-reaction to other rickettsial strains varied from patient to patient, but a particular pattern of cross-reaction was consistently observed in serial sera from the ... | 1976 | 815267 |
| alterations in plasma copper, zinc, amino acids, and seromucoid during rocky moutain spotted fever in guinea pigs. | guinea pigs inoculated with virulent rickettsia rickettsii responded with a significant increase in plasma copper concentration within 1 day, preceding fever and detectable rickettsemia by 2 and 4 days, respectively. a decrease in serum zinc concentration coinciding with peak rickettsemia was detectable on day 5. evidence of altered host nitrogen metabolism during this illness included a doubling of plasma seromucoid concentration and a significant rise in the plasma phenylalanine/tyrosine ratio ... | 1976 | 817295 |
| antibody response to rocky mountain spotted fever. | various techniques were compared to determine the most sensitive method for detection of rocky mountain spotted fever antibody. a radiometabolic technique for detection of rocky mountain spotted fever antibody is also described. in infected monkeys, the fluorescent antibody technique yielded the earliest evidence of seroconversion; with some monkeys the microagglutination procedure was equally effective. the fluorescent antibody and microagglutination measurements showed higher titers than those ... | 1976 | 819455 |
| pathology of experimental rocky mountain spotted fever in rhesus monkeys. | rhesus monkeys were inoculated intravenously or intraperitoneally with various numbers of rickettsia rickettsii. monkeys that were given more than 10(4) organisms intravenously died on days 3-6 after inoculation, whereas those given less than 10(3) intravenously or more than 10(4) intraperitoneally died on day 7 or later. there was no significant difference in incidence of lesions between the early- and late-death groups. vasculitis and thrombosis occurred most frequently in the nares, pinna of ... | 1976 | 820052 |
| changes in blood serum constituents and hematologic values in macaca mulatta with rocky mountain spotted fever. | forty-seven male macaca mulatta, 3 to 4 kg weight, were inoculated intravenously or subcutaneously with various doses of yolk sac-grown rickettsia rickettsii. thirty-four macaques became febrile and exhibited signs of infection ranging from transient illness with a few days of fever to severe illness with subsequent death. the rash appeared more frequently in the macaques inoculated subcutaneously. febrile macaques that survived had leukocytosis, with concomitant neutrophilia. febrile macaques t ... | 1976 | 820224 |
| rickettsiae belonging to the spotted fever group from ticks in the tribec mountains. | rickettsiae of the spotted (sf) group were found in a selected locality of the tribec mountains in southern slovakia in 1974. the rickettsiae were found in 10.8% of females and 6.1% of males of haemaphysalis inermis and 7.3% of females and 4.8% of males of ixodes ricinus. no significant differences in the incidence were observed during the year. coxiella burneti was found in 1 male and 4 females of h. inermis and 1 male of i. ricinus in ad adjacent locality. antibodies against rickettsiae of the ... | 1976 | 820614 |
| proteins of typhus and spotted fever group rickettsiae. | purified radioactive rickettsiae were obtained from irradiated and cycloheximide-inhibited l cells, and their proteins were analyzed by polyacrylamide gel electrophoresis. rickettsial species could be distinguished by comparing the relative mobilities of constituent proteins after migration of two differentially labeled preparations in a single gel. distinct differences were observed in gel patterns of rickettsiae from the typhus and spotted fever groups, as well as with different species within ... | 1976 | 820639 |
| functional and morphologic changes during experimental rocky mountain spotted fever in guinea pigs. | experimental rocky mountain spotted fever was studied in guinea pigs following intraperitoneal inoculation of 10(7) rickettsia rickettsii. after a 2-day incubation period, animals developed fever, progressive emaciation, and scrotal swelling with necrosis. vasculitis, with increased small vessel permeability for colloidal carbon, was evident in cremaster muscles as early as 1 day after inoculation. inflammatory changes in vessels became progressively more severe as numbers of circulating rickett ... | 1976 | 822237 |
| demonstration of rickettsia rickettsii in the rhesus monkey by immune fluorescence microscopy. | indirect immune fluorescence was used to detect rickettsiae in the tissues of a primate inoculated subcutaneously with rickettsia rickettsii. rickettsiae were identified by indirect immuno fluorescence predominantly in skin, skeletal muscle, scrotum, testicles, nares, heart, kidney, liver, brain, spleen, pancreas, and larynx. cell culture assay confirmed the presence of infectious organisms in those specimens. | 1976 | 823168 |
| effect of vaccination schedule on immune response of macaca mulatta to cell culture-grown rocky mountain spotted fever vaccine. | the effect of vaccination schedule on the immune response of macaca mulatta to formalin-inactivated chicken embryo cell culture (cec)-grown rickettsia rickettsii vaccine was studied. schedules consisted of inoculation on day 1 only, on days 1 and 15, on days 1 and 30, on days 1, 8, and 15, or on days 1, 15, and 45. humoral antibody measured by microagglutination and indirect immunofluorescence and resistance to challenge with 10(4) plaque-forming units of yolk sac-grown r. rickettsii were assess ... | 1976 | 823173 |
| new assay of protective activity of rocky mountain spotted fever vaccines. | areas under the fever curves of guinea pigs inoculated with rocky mountain spotted fever vaccine over a restricted dose range and infected with a standardized dose of rickettsia rickettsii varied linearly with log10 dose of vaccine. a calculator was programmed to plot fever curves and calculate the vaccine dose that reduced the fever of infected animals by 50%. | 1976 | 823177 |
| prompt confirmation of rocky mountain spotted fever: identification of rickettsiae in skin tissues. | rickettsia rickettsii were identifiable by the immunofluorescence technique in skin specimens obtained by biopsy on days 4 and 8 of illness from patients with rocky mountain spotted fever. the immunofluorescence technique is regarded as a practical means of confirming the diagnosis during the early stages of illness before positive serologic reactions can be obtained. | 1976 | 824374 |
| infection cycle of rickettsia rickettsii in chicken embryo and l-929 cells in culture. | the infection cycle of rickettsia rickettsii, studied in slide chamber cultures of chicken embryo and l-929 cells, was found to be complex and did not conform to a one-step growth cycle. initial uptake kinetics resembled those established for rickettsia prowazekii, but subsequent events showed very marked differences. intracytoplasmic growth commenced exponentially without measurable lag. however, very soon after infection, intracytoplasmic rickettsiae began to escape from the host cell into the ... | 1976 | 825463 |
| study of rickettsioses in slovakia. iii. experimental infection of apodemus flavicollis melch. by rickettsiae of the spotted fever (sf) group isolated in slovakia. | experimental studies of infection of apodemus flavicollis, and for comparison of rickettsiaemia in clethrionomys glareolus and of susceptibility and antibody formation in white mice, with rickettsiae of the sf group isolated in slovakia, gave the following results: the species a. flavicollis reacted by the formation of antibodies on subcutaneous administration of rickettsiae, strain b, in an amount of 10(0.5) eid50/0.25 ml, whereas the white mouse only in an amount of 10(2.5). rickettsiae, strai ... | 1976 | 825571 |
| vectors and vertical transmission: an epidemiologic perspective. | | 1975 | 829470 |
| mechanisms of transovarial infection of spotted fever rickettsiae in ticks. | | 1975 | 829476 |
| studies on the pathogenesis of rickettsia rickettsii in the dog: clinical and clinicopathologic changes of experimental infection. | beagle dogs inoculated with the agent of rocky mountain spotted fever, rickettsia rickettsii, developed a clinical syndrome that extended from febrile exanthema to death and appeared to be dose related. infected dogs were anorectic and lethargic and developed cutaneous lesions characteristic of rocky mountain spotted fever, including petechia, ecchymosis, edema, and necrosis. hematologic changes after inoculation included anemia, leukopenia proceeding to leukocytosis, and thrombocytopenia. chang ... | 1977 | 879582 |
| interaction between coxiella burnetii and guinea pig peritoneal macrophages. | the phagocytosis and subsequent degradation of phase i and ii coxiella burnetii by macrophages obtained from immune and nonimmune guinea pigs were compared. phase i rickettsiae were more resistant to phagocytosis than were phase ii organisms. there was no significant difference in the percentage of phagocytosis of either phase of rickettsiae by macrophages from immune or nonimmune animals. after ingestion, phase i and ii organisms pretreated with normal serum multiplied and destroyed normal macr ... | 1976 | 971955 |
| high resolution polyacrylamide gradient gel electrophoresis. | a method was developed for high resolution electrophoresis of proteins in linear gradient (3 to 30%) polyacrylamide gel rods in a neutral phosphate buffer containing 0.1% sodium dodecyl sulfate. well-defined protein zones were observed and improved resolution was attained especially for low molecular weight proteins in preparations containing a variety of polypeptides, e.g. viruses that are often separated by continuous gel methods. electropherograms of continuous (8%) and gradient (3 to 30%) ge ... | 1976 | 1005359 |
| dynamic cisternography in normal dogs and in human beings. | cisternography and ventriculography were performed in five normal anesthetized dogs to study the dynamics of tracer dispersal. indium 111-diethylenetriaminepentaacetic acid was injected into lateral ventricles, cisterna magna, thoracic subarachnoid space, and lumbar sac. the patterns of dispersal from each injection site support a nonflow dispersal mechanism with a tendency toward ultimate equilibrium distribution throughout the cerebrospinal fluid. quantitative data in humans complement observa ... | 1976 | 1082560 |
| comparative morphology of genital cones of genus ancylostoma dubini, 1843. | male worms of ancylostoma braziliense, a. ceylanicum, a. kusimaense, a. malayanum, a. duodenale, a. caninum, a. tubaeforme, agriostomum vryburgi and cyclodontostomum purvisi have the external appendages beside the anogenital aperture. these anogenital structures are morphologically similar and are assumed to be homologous among the species with three pairs of teeth or more. in hookworms with two pairs of teeth, the anogenital structures are complex. anogenital features can be used as a taxonomic ... | 1975 | 1166351 |
| [spotted fever: isolation of rickettsia from a skin biopsy sample]. | a 2 years old child living in an area of the state of são paulo, known in the past as endemic for rickettsiosis developed clinical evidences of spotted fever after a tick bite. rickettsiae were isolated from guinea pigs inoculated with a skin homogenate. in sera tested by indirect immunofluorescence with rickettsia rickettsii standard antigen, igg specific antibody titers raised from 1:512 in the first sample to 1:2048 in the third one; igm specific antibody titer was 1:128 in the three samples. ... | 1992 | 1307409 |
| determination of genome sizes of rickettsia spp. within the spotted fever group, using pulsed-field gel electrophoresis. | the chromosome lengths of six spotted fever group rickettsia species (rickettsia rickettsii, r. conorii, r. rhipicephali, r. sibirica, r. australis, and r. akari) were estimated by pulsed-field gel electrophoresis. the genome size of r. rickettsii was about 2,100 kb, but the chromosome lengths of the five other species were, surprisingly, much lower and ranged between 1,200 and 1,300 kb. | 1992 | 1331027 |
| rickettsia rickettsii induces superoxide radical and superoxide dismutase in human endothelial cells. | human endothelial cells infected with rickettsia rickettsii, the etiological agent of rocky mountain spotted fever, undergo striking morphological changes to the endoplasmic reticulum-outer nuclear envelope complex. these changes are accompanied by concurrent accumulation of intracellular peroxides. both of these findings are consistent with the notion that cells undergo some form of oxidative stress. since oxidant injury is often initiated or mediated through oxygen radicals, we examined supero ... | 1992 | 1333449 |
| susceptibility of rickettsia conorii, r. rickettsii, and coxiella burnetii to pd 127,391, pd 131,628, pefloxacin, ofloxacin, and ciprofloxacin. | plaque formation and dye uptake assays were used to measure the mics of pd 127,391 and pd 131,628 against rickettsia species. the mics of pd 127,391 were 0.25 microgram/ml for rickettsia rickettsii and 0.125 to 0.25 microgram/ml for rickettsia conorii. the mics of pd 131,628 were 0.25 to 0.5 microgram/ml for r. rickettsii and 0.5 microgram/ml for r. conorii. as determined by the shell vial technique, 15 strains of coxiella burnetii were susceptible to pd 127,391 and pd 131,628 (mic, < or = 1 mic ... | 1992 | 1336950 |
| african tick-bite fever: a new spotted fever group rickettsiosis under an old name. | | 1992 | 1357393 |
| specificity of a tumor marker (ca54/61) and its individual epitopes recognized by monoclonal antibodies, ma54 and ma61, in human tumor patients. | the specificity of a tumor marker (ca54/61) and its individual epitopes (ca54 and ca61) recognized by monoclonal antibodies (ma54 and ma61) and expressed by the same tumor marker were studied. serum levels of ca54 and ca61 were compared with that of ca54/61. in lung adenocarcinoma and ovarian carcinoma, the positive rates of ca61 (42% and 68%) were higher than those of ca54 (32% and 32%) and similar to those of ca54/61 (45% and 74%). the serum levels of ca54 and ca61 showed a significant correla ... | 1992 | 1383482 |
| a cluster of tick-borne infections: association with military training and asymptomatic infections due to rickettsia rickettsii. | during the spring of 1989, 86 members of a military unit from the state of maryland, usa, participated in two-week-long training manoeuvres in the states of arkansas (location fc) and virginia (location faph). acute febrile illnesses due to infections with two tick-borne pathogens, rickettsia rickettsii and ehrlichia sp., were confirmed serologically in 2 initial cases who were admitted to the hospital. a seroepidemiological investigation among unit members found an additional 17 of 109 individu ... | 1992 | 1412666 |
| demonstration and partial characterization of antigens of rickettsia rhipicephali that induce cross-reactive cellular and humoral immune responses to rickettsia rickettsii. | the relatively unrelated spotted fever group rickettsia rickettsia rhipicephali conferred on guinea pigs protective immunity against challenge with virulent r. rickettsii. immunity was conferred at all doses of r. rhipicephali used in the study. because of the serologic unrelatedness of these two rickettsiae, determined by the use of microimmunofluorescence and other serological assays, further studies were performed to define the nature of the immune response elicited by r. rhipicephali and the ... | 1992 | 1452343 |
| characterization and comparison of australian human spotted fever group rickettsiae. | the microbiological and molecular characteristics of the rickettsiae isolated from humans with queensland tick typhus (qtt) caused by rickettsia australis and the recently described flinders island spotted fever (fisf) were compared. clinically and serologically, the diseases are similar. cell culture reveals differences in the plaque-forming abilities of the isolates. characterization of the gene encoding the genus-specific 17-kda antigen of r. australis revealed a unique nucleotide sequence un ... | 1992 | 1452660 |
| rocky mountain "spotless" and "almost spotless" fever: a wolf in sheep's clothing. | in 10 (10.8%) of 93 laboratory-confirmed or probable cases of rocky mountain spotted fever seen at duke university medical center from 1969 to 1991, illness without rash or fleeting or atypical skin eruptions were noted. data from these 10 cases and 33 similar previously reported cases of rocky mountain "spotless" or "almost spotless" fever support the premise that human rickettsia rickettsii infection has a broader spectrum than that indicated by its classic description. sixty-one percent of pa ... | 1992 | 1520791 |
| detection of rickettsia rickettsii in saliva, hemolymph and triturated tissues of infected dermacentor andersoni ticks by polymerase chain reaction. | the technique of polymerase chain reaction (pcr) is potentially superior to existing methods for detecting rickettsial infections in ticks. for this reason, we developed assays for identifying rickettsial infections in ticks by pcr. our assays amplified a 500 bp fragment from the gene encoding the romp b protein of rickettsia rickettsii. the selected primers amplified fragments of the predicted size from all spotted fever group rickettsiae (r. rickettsii, r. parkeri, r. conorii, r. sibirica) tes ... | 1992 | 1528203 |
| analysis of functional domains of endoglucanases from clostridium cellulovorans by gene cloning, nucleotide sequencing and chimeric protein construction. | the nucleotide sequence of engd, an endo-beta-1,4-glucanase gene from clostridium cellulovorans was determined (genbank accession no. m37434). the cooh-terminal part of the gene product, engd, contained a thr-thr-pro repeated sequence followed by a region that has homology to the exoglucanase of cellulomonas fimi. engd and engb, another c. cellulovorans endoglucanase, show 75% amino acid sequence homology at their nh2-termini, in contrast to their carboxyterminal domains which show no homology. ... | 1992 | 1538700 |
| cell-mediated immune responses of adults to vaccination, challenge with rickettsia rickettsii, or both. | as a part of a study to evaluate a formalin-killed rickettsia rickettsii vaccine, lymphoproliferative (lt) and delayed-type hypersensitivity (dth) skin test responses to killed r. rickettsii were measured as correlates of cell-mediated immunity in volunteers who were vaccinated, challenged with r. rickettsii, or both. we detected lt responses in 26 (51%) of 51 volunteers after vaccination. after challenge, six of six unvaccinated volunteers and 12 of 16 vaccinated volunteers developed rocky moun ... | 1992 | 1539743 |
| analysis of leptospira spp., leptonema illini, and rickettsia rickettsii for the 39-kilodalton antigen (p39) of borrelia burgdorferi. | five serovars of leptospira interrogans, leptospira biflexa, leptonema illini, and rickettsia rickettsii were examined and found not to contain the 39-kda antigen (p39) of borrelia burgdorferi, the lyme disease spirochete. the specificity of this antigen and its reactivity with human lyme disease sera should exclude the possibility of false-positive serum samples from patients having had either leptospirosis or rocky mountain spotted fever, as well as tick-borne relapsing fever and syphilis, as ... | 1992 | 1551994 |
| synthetic cephalosporins. viii. synthesis and antibacterial activity of cp6232, a new anti-pseudomonal cephalosporin. | | 1992 | 1592693 |
| spotted fever rickettsiae in ticks from the northern sinai governate, egypt. | a field study was initiated in 1988 to investigate whether spotted fever group rickettsiae occur in geographic areas in egypt that are adjacent to an area in the southern israeli negev that has a defined focus of spotted fever disease. ticks were collected from dogs, sheep, and camels at four study sites in the northern sinai. tick hemolymph was processed for rickettsial detection by staining with fluorescein isothiocyanate-conjugated antibody to rickettsia rickettsii. of the 442 hemolymphs exam ... | 1992 | 1599048 |
| gelatin particle agglutination test for early serodiagnosis of japanese spotted fever. | a gelatin particle agglutination (pa) test for japanese spotted fever has been developed. gelatin particles were sensitized with a sonicated causative rickettsia and used as antigens. the antibodies by pa test were detected as early as days 4-7 after the onset, whereas those by indirect immunoperoxidase (ip) test were detected after days 8-11. in addition, pa titers were higher than ip titers before days 20-23. the agglutinins detected by pa test were proven to be igm because they were all sensi ... | 1992 | 1603001 |
| ultrastructural study of the response of cells infected in vitro with causative agent of spotted fever group rickettsiosis in japan. | the response of host cells l929 infected with causative agent of spotted fever group (sfg) rickettsiosis in japan, the katayama strain, was studied by electron microscopy. the rickettsiae penetrated the cytoplasm and multiplied here and after prolonged incubation progressed into the dilated cisternae of rough endoplasmic reticulum (rer), the perinuclear space, the deep invaginated nuclear membrane, and then the nucleoplasm of the host cells. the intranuclear rickettsiae showed different states: ... | 1992 | 1610553 |
| penetration of host cells by rickettsia rickettsii appears to be mediated by a phospholipase of rickettsial origin. | internalization of obligate intracellular bacteria belonging to the genus rickettsia by eukaryotic cells requires participation of both the parasitized host and the microorganism. the term "induced phagocytosis" has been used specifically to describe the entry of rickettsia prowazekii, although a similar mechanism is likely for r. rickettsii. a role for a phospholipase in the internalization process has been proposed for both of these organisms, with the strongest supporting evidence provided fo ... | 1992 | 1612741 |
| oxidative inactivation of alpha 1-proteinase inhibitor by alveolar macrophages from healthy smokers requires the presence of myeloperoxidase. | the aim of this work was to study the ability of human alveolar macrophages (am) of 10 healthy smokers to inactivate alpha 1-proteinase inhibitor (alpha 1pi). purified alpha 1pi was incubated for 45 min, with human alveolar macrophages before and after stimulation by phorbol myristate acetate (pma) or opsonized zymosan. as a positive control, the same experiments were performed in parallel with blood human neutrophils (pmn). results are expressed as percentage of inactivation of alpha 1pi as eva ... | 1991 | 1657063 |
| efficacy of chloramphenicol, enrofloxacin, and tetracycline for treatment of experimental rocky mountain spotted fever in dogs. | dogs were experimentally inoculated with rickettsia rickettsii to characterize the comparative efficacies of chloramphenicol, enrofloxacin, and tetracycline for the treatment of rocky mountain spotted fever (rmsf). all three antibiotics were equally effective in abrogating the clinical, hematologic, and vascular indicators of rickettsial infection. antibiotic treatment for 24 h was sufficient to decrease the rickettsemia to levels below detection by vero cell culture. early treatment with all th ... | 1991 | 1666498 |
| genotypic identification of rickettsiae and estimation of intraspecies sequence divergence for portions of two rickettsial genes. | dna sequences from specific genes, amplified by the polymerase chain reaction technique, were used as substrata for nonisotopic restriction endonuclease fragment length polymorphism differentiation of rickettsial species and genotypes. the products amplified using a single pair of oligonucleotide primers (derived from a rickettsial citrate synthase gene sequence) and cleaved with restriction endonucleases were used to differentiate almost all recognized species of rickettsiae. a second set of pr ... | 1991 | 1671856 |
| dna polymorphism in the conserved 190 kda antigen gene repeat region among spotted fever group rickettsiae. | the 190 kda protein gene of r. rickettsii (r strain) contains a large region of tandemly arranged repeats. homologous sequences were discovered within the genomes of all but one species of spotted fever group rickettsiae. southern blots showed restriction fragment length polymorphisms between species of rickettsiae indicating structural differences among repeat regions. further analysis using polymerase chain reaction techniques indicated the repeat regions vary in size among rickettsiae. in add ... | 1991 | 1677592 |
| genetics of rickettsiae. | classical genetic approaches useful with free-living bacteria are difficult to apply to the rickettsiae. although rickettsial mutants have been isolated over the years, the genetic basis of these mutants is unknown, limiting their usefulness. the application of molecular biological techniques to rickettsial studies has provided the opportunity to isolate and study specific genes. genes encoding metabolic enzymes from rickettsiae were cloned in escherichia coli and shown to retain their regulator ... | 1991 | 1679397 |
| promoter structure and expression of the 155-kda surface antigen gene of rickettsia rickettsii. | | 1990 | 1696076 |
| species-specific monoclonal antibodies to rickettsia japonica, a newly identified spotted fever group rickettsia. | a total of 192 hybridomas were developed from mice immunized with rickettsia japonica, a newly identified spotted fever group rickettsia pathogenic for humans. of these hybridomas, 101 were species specific, 37 were spotted fever group reactive, and the other 54 were also reactive with one or more of the other pathogenic species of spotted fever group rickettsiae, rickettsia akari, rickettsia australis, rickettsia conorii, rickettsia rickettsii, and rickettsia sibrica. seven of the species-speci ... | 1990 | 1696280 |
| polypeptides constituting the antigenic basis for identification of rickettsia sibirica species by the standard serotyping method for spotted fever group rickettsiae. | the antigens of rickettsia sibirica, rickettsia rickettsii, and rickettsia conorii were examined by western immunoblots and the standard immunofluorescent serotyping assay with mouse anti-rickettsia sibirica serum that is used for determining the species identification of spotted fever group rickettsiae. serum was employed prior to absorption and after absorption with purified native or heated rickettsia sibirica. the unabsorbed antiserum, the antiserum absorbed with heated rickettsia sibirica a ... | 1990 | 1697727 |
| iloprost inhibits neutrophil-induced lung injury and neutrophil adherence to endothelial monolayers. | we hypothesized that iloprost, a long-acting prostacyclin analog, would inhibit neutrophil (pmn)-induced lung injury and decrease pmn adherence to vascular endothelium. human pmns infused into isolated buffer-perfused rat lungs subsequently stimulated with phorbol myristate acetate (pma) resulted in lung injury as assessed by the accumulation of [125i]bovine serum albumin (125i-bsa) in lung parenchyma and alveolar lavage fluid. addition of iloprost to the lung perfusate, prior to activation of t ... | 1990 | 1698399 |
| comparative susceptibility to mouse interferons of rickettsia tsutsugamushi strains with different virulence in mice and of rickettsia rickettsii. | three strains of rickettsia tsutsugamushi (karp, gilliam, and ta716, representing three virulence types in mice) were examined for their sensitivity to the inhibitory effects of recombinant gamma interferon (ifn-gamma) and purified ifn-alpha/beta in two cultured mouse fibroblast cell lines. the susceptibilities of another species, rickettsia rickettsii, and of encephalomyocarditis virus (emcv) were also tested for comparative purposes. ifn-gamma inhibited rickettsial replication in only one of t ... | 1991 | 1718869 |
| the 120 kilodalton outer membrane protein (romp b) of rickettsia rickettsii is encoded by an unusually long open reading frame: evidence for protein processing from a large precursor. | a rickettsia rickettsii outer surface membrane protein (romp b), of an apparent molecular mass of 120 kilodaltons, is a major surface antigen of the rickettsiae that displays genus, species, and sub-species specific antigenic determinants. the 5' portion of this gene was found to be unstable in plasmids, but was stably cloned in a lambda vector. the nucleotide sequence of the 5' terminus has been determined, thus completing the dna sequence of the entire gene. genetic analysis revealed an unusua ... | 1991 | 1724278 |
| evidence for proteolytic cleavage of the 120-kilodalton outer membrane protein of rickettsiae: identification of an avirulent mutant deficient in processing. | the 120-kda rickettsial outer membrane protein (rompb) is encoded by a gene with the capacity to encode a protein of approximately 168 kda. the carboxy-terminal end of the molecule is apparently cleaved to yield 120- and 32-kda products. both polypeptides are surface exposed and remain associated with the outer membrane of intact rickettsiae. all species of rickettsiae examined display similar cleavage of rompb. comparison of diverse species of rickettsiae demonstrate a conserved n terminus of t ... | 1992 | 1729180 |
| linkage of bipolar affective disorders to markers on chromosome 11p is excluded in a second lateral extension of amish pedigree 110. | linkage between markers on chromosome 11p and bipolar affective disorders can be excluded in a second large lateral extension of the original amish pedigree 110. these results, together with previous negative linkage findings, suggest that there is not one single gene on 11p conferring susceptibility for bipolar affective disorders among the old order amish. | 1991 | 1774072 |
| lactoferrin levels and unsaturated iron-binding capacity in colostrum of brazilian women of two socioeconomic levels. | the objective of this study was to compare lactoferrin (lf) levels and unsaturated iron-binding capacity (uibc) of colostrum from brazilian women aged 20-38 years of low (n = 28) and high (n = 15) socioeconomic level. both groups received a similar amount of supplemental iron (25-65 mg/day) during pregnancy. we also determined the relationship between lf levels and uibc, and between these components and maternal iron status. both groups (low and high socioeconomic level) showed adequate and simi ... | 1991 | 1797281 |
| lactoferrin levels and unsaturated iron-binding capacity in colostrum of brazilian women of two socioeconomic levels. | the objective of this study was to compare lactoferrin (lf) levels and unsaturated iron-binding capacity (uibc) of colostrum from brazilian women aged 20-38 years of low (n = 28) and high (n = 15) socioeconomic level. both groups received a similar amount of supplemental iron (25-65 mg/day) during pregnancy. we also determined the relationship between lf levels and uibc, and between these components and maternal iron status. both groups (low and high socioeconomic level) showed adequate and simi ... | 1991 | 1797281 |
| cloning, expression and sequence analysis of the gene encoding the 120 kda surface-exposed protein of rickettsia rickettsii. | | 1991 | 1809847 |
| [anti-inflammatory activities and effect of rhizoma alismatis on immune system]. | 10 and 20 g/kg ig of rhizoma alismatis (ra) markedly decrease the clearance rate of charcoal particles in mice, but have no significant effect on the weight of immune organs, or on the content of serum antibody hemolysin and immunoglobulin g and the delayed footpad edema induced by sheep red blood cell. the contact dermatitis of mouse pinnae immunized with dinitrochlorobenzene is inhibited by ra when it is given before challenge. in addition, ra 20g/kg suppresses the swelling of mouse rinnae ind ... | 1991 | 1820797 |
| rickettsia rickettsii infection of cultured endothelial cells induces release of large von willebrand factor multimers from weibel-palade bodies. | the clinical manifestations of rocky mountain spotted fever (rmsf) result from rickettsia rickettsii (r rickettsii) infection of endothelial cells and are mediated by pathologic changes localized to the vessel, including in situ thrombosis and tissue ischemia. this study uses in vitro infection of cultured human umbilical vein endothelial cells with r rickettsii to test the hypothesis that such infection induces von willebrand factor (vwf) release from weibel-palade bodies, a process that could ... | 1991 | 1824255 |
| effects of venous hypertension on rabbit free flap survival. | a two-stage project was developed to study the effect of increased venous pressure on blood flow and survival in microvascular free tissue transfers. a rabbit epigastric fasciocutaneous free flap model was used. the blood flow in the rabbit epigastric free flap is 1.07 +/- 0.06 ml/min. the average venous pressure is 8.6 +/- 1.7 cm water. a logarithmic relationship between blood flow and venous pressure was demonstrated, with a statistically significant decrease in blood flow to 20-35% of the con ... | 1991 | 1895934 |
| flinders island spotted fever: a newly recognised endemic focus of tick typhus in bass strait. part 2. serological investigations. | twenty-six cases of a spotted-fever-like illness have been identified on flinders island, tasmania, over a 17 year period. these patients and 335 healthy persons from the island were investigated serologically using the weil-felix agglutination test (proteus sp. antigens ox2, ox19, oxk) and rickettsia-specific microimmunofluorescence. the antigens used in these latter tests comprised one member of the typhus group (rickettsia typhi) and three members of the spotted fever group (rickettsia ricket ... | 1991 | 1898756 |
| fatal rocky mountain spotted fever in maryland--1901. | | 1991 | 1899275 |
| in vitro susceptibilities of coxiella burnetii, rickettsia rickettsii, and rickettsia conorii to the fluoroquinolone sparfloxacin. | in vitro susceptibilities of rickettsia rickettsii, rickettsia conorii, and coxiella burnetii to the new fluoroquinolone sparfloxacin (at-4140; rp 64206) were determined. plaque and dye uptake assays were used to measure the mics against r. rickettsii and r. conorii. the susceptibilities of c. burnetii nine mile and q 212 were determined in two acute-infection models and in two chronic-infection models. the mics were 0.125 to 0.25 microgram/ml for r. rickettsii and 0.25 to 0.5 microgram/ml for r ... | 1991 | 1901703 |
| fine needle aspiration biopsy diagnosis of tuberculous lymphadenitis in patients with and without the acquired immune deficiency syndrome. | all bellevue hospital cases from a recent 27-month period whose fine needle aspiration (fna) samples of cervical or supraclavicular masses showed acute and/or granulomatous inflammation were reviewed. the 30 patients included 8 with the acquired immune deficiency syndrome (aids), 3 with the aids-related complex (arc), 2 with aids risk factors and 17 without known risk factors for aids. of these, mycobacterial infections had been diagnosed in 22 patients: 18 by cultures positive for mycobacterium ... | 1991 | 1904179 |
| rocky mountain spotted fever. | rocky mountain spotted fever, the most prevalent rickettsial disease in the united states, is one of the most severe of all infectious diseases. it remains an important infectious disease because of its prevalence, the difficulty of clinical diagnosis, potentially fatal outcome, and lack of a widely available sensitive and specific diagnostic test during the acute stage of the illness. | 1991 | 1904897 |
| dna base composition of rickettsia tsutsugamushi determined by reversed-phase high-performance liquid chromatography. | the dna base composition of rickettsia tsutsugamushi was determined by reversed-phase high-performance liquid chromatography and compared with that of rickettsia rickettsii. the g+c contents were 28.1 to 30.5 mol% for r. tsutsugamushi and 32.1 mol% for r. rickettsii. | 1991 | 1906730 |
| [gas chromatograms of fatty acids from rickettsia and their similarities]. | fatty acid compositions of 7 strains of rickettsia were analyzed by a on-line gc/ms system. these strains were r. prowazekii e, r. conorii simko, r. rickettsii r, r. sibirica barbash and 246, r. sinkiangensis jinghe, and r. heilungkiangensis 54. the samples were purified by means of the concentrated salt-ether method. there were about 50 peaks in the fatty acid profiles, and 16 of these peaks were determined, i.e. c22:0, c19:0, c18:0, c18:1, c18:2, c17:0, c16:0, c16:1, 3-oh-c14:0, c15:0, c14:0, ... | 1991 | 1907779 |
| some contributions of electron microscopy to the study of the rickettsiae. | electron microscopy has provided valuable insights into the study of rickettsiae as intracellular parasites from several important perspectives. this tool has allowed researchers to delineate the fine structural features of these organisms and to show that they truly resemble free-living bacteria. furthermore, it has been shown that there are subtle, but distinct differences in the outer envelope structure of some members of the genus rickettsia that may explain reported differences in tinctoria ... | 1991 | 1909242 |
| antigenic relationships among the rickettsiae of the spotted fever and typhus groups. | using immunoblots to analyze antigenic relationships among the pathogenic spotted fever and typhus group rickettsiae, i found that the rickettsial lipopolysaccharide (lps) was a group-specific antigen. all the rickettsiae examined had 135-kda and 58-kda protein antigens. the spotted fever rickettsiae and rickettsia canada had, in addition, 190-kda protein antigens which were antigenic analogs of previously described protective antigens of r. conorii and r. rickettsii. | 1991 | 1916232 |
| heparin protects human endothelial cells infected by rickettsia rickettsii. | routine culture of endothelial cells currently includes the use of heparin, which significantly reduces cell doubling time and increases cell population size. heparin protects cultured arterial endothelial cells from damage by toxic oxygen metabolites produced by the action of xanthine and xanthine oxidase. because of our hypothesis implicating free radicals in cell injury caused by rickettsia rickettsii, we have carried out a series of experiments to examine the effects of heparin on injury to ... | 1991 | 1937810 |
| site and strand specificity of uvb mutagenesis in the sup4-0 gene of yeast. | | 1991 | 2000411 |
| false-positive results in serologic tests for rocky mountain spotted fever during pregnancy. | the data from this study demonstrate that false-positive results from tests for rocky mountain spotted fever increase with the duration of pregnancy. the sera of 4.0% (2/50) of women in their first trimester of pregnancy, 10.9% (5/46) in their second trimester, and 12.1% (12/99) in their third trimester yielded false-positive results from latex agglutination assays for rickettsia rickettsii infections. the cause of these false-positive results was not determined by this study. these false-positi ... | 1991 | 2000516 |
| rocky mountain spotted fever. | rocky mountain spotted fever is an endemic tickborne disease found throughout the united states and other regions of the world. exposure may result in a spectrum of disease from subclinical infection to severe or fatal multiorgan collapse. the disease is maintained in nature in ixodid tick vectors and their hosts. the most important ticks in the united states are dermacentor variabilis and dermacentor andersoni. small mammals are the natural reservoirs in the wild. dogs become infected when a ti ... | 1991 | 2014623 |
| effects of amino acid infusion on renal hemodynamics. role of endothelium-derived relaxing factor. | ingestion of protein or intravenous infusion of amino acids acutely elevates glomerular filtration rate (gfr) and renal plasma flow (rpf) by unknown mechanisms. endothelium-derived relaxing factor (edrf), now known to be nitric oxide derived from metabolism of l-arginine, participates in local regulation of vascular tone. to investigate the hypothesis that edrf may participate in the renal vasodilatation and increased gfr after amino acid infusion, we characterized the effect of inhibition of ed ... | 1991 | 2045148 |
| [rocky mountain spotted fever]. | after returning from a holiday in the usa a 24-year-old man fell ill with diarrhoea, high fever and marked rash including the palms of the hands and soles of the feet. when a history of a tick bite in the usa was elicited, a rickettsial infection was suspected. treatment with doxycycline, 100 mg twice daily, was instituted finally and the fever slowly resolved. the patient became completely well again within four weeks. serological tests confirmed the diagnosis of rocky mountain spotted fever. | 1990 | 2104798 |
| vascular permeability and coagulation during rickettsia rickettsii infection in dogs. | the vascular permeability of the ocular fundus, alterations in the coagulation system, and plasma concentrations of thromboxane b2 (txb2) and 6-keto-prostaglandin f1 alpha (6-keto-pgf1 alpha) were studied in dogs following intradermal inoculation with 5 x 10(5) tcid50 of rickettsia rickettsii. twenty-four to 48 hours after the onset of fever and rickettsemia, multifocal areas of retinal vasculitis were evident, which corresponded to areas of altered vascular permeability demonstrated by fluoresc ... | 1990 | 2105679 |