| site-directed mutations of thermostable direct hemolysin from grimontia hollisae alter its arrhenius effect and biophysical properties. | recombinant thermostable direct hemolysin from grimontia hollisae (gh-rtdh) exhibits paradoxical arrhenius effect, where the hemolytic activity is inactivated by heating at 60 °c but is reactivated by additional heating above 80 °c. this study investigated individual or collective mutational effect of tyr53, thr59, and ser63 positions of gh-rtdh on hemolytic activity, arrhenius effect, and biophysical properties. in contrast to the gh-rtdh wild-type (gh-rtdh(wt)) protein, a 2-fold decrease of he ... | 2011 | 21494434 |
| analysis of gyrb and toxr gene sequences of vibrio hollisae and development of gyrb- and toxr-targeted pcr methods for isolation of v. hollisae from the environment and its identification. | isolation of vibrio hollisae strains, particularly from the environment, is rare. this may be due, in part, to the difficulty encountered when using conventional biochemical tests to identify the microorganism. in this study, we evaluated whether two particular genes may be useful for the identification of v. hollisae. the two genes are presumed to be conserved among the bacterial species (gyrb) or among the species of the genus vibrio (toxr). a portion of the gyrb sequence of v. hollisae was cl ... | 2000 | 10919814 |
| interactions between response stereotypy and memory strategies on the eight-arm radial maze. | three groups of water-deprived rats collected water from the ends of the 8-arm radial maze. sighted subjects, and subjects blinded either with or without pre-enucleation experience on the radial maze, all retrieved the water efficiently. most of the subjects exhibited the same response stereotypy, regularly choosing 8 adjacent arms of the maze, then stopping in the center of the maze. the strategies underlying this performance were analysed by interrupting trials and rotating the maze 180 degree ... | 1986 | 3954863 |
| liposome as an adjuvant for the production of estradiol antibodies. | mice immunized with liposome associated estradiol-bovine serum albumin conjugate (e2-bsa) showed a significantly higher estradiol-specific immune response than did mice injected with the free conjugate. the conjugation of estradiol with bsa is imperative, since injection of liposomes with estradiol in the lipid bilayer was found to be ineffective in inducing an immune response. entrapment of e2-bsa in neutral and positively charged liposomes was found to be a potent mode of immunostimulation, ev ... | 1987 | 3666803 |
| bone mineral metabolism in experimental diabetes mellitus: osteocalcin as a measure of bone remodeling. | the etiology of diabetic osteopenia has not been established. the value of serum osteocalcin (bgp) as a marker of the bone abnormalities and the possible role of the polyol pathway in diabetic osteopenia were investigated. three groups of rats were studied over 7 weeks: group d (n = 12), rats with streptozotocin (55 mg/kg)-induced diabetes given saline by gavage; group ds (n = 12), rats with streptozotocin-induced diabetes given the aldose reductase inhibitor sorbinil (25 mg/kg) daily by gavage; ... | 1988 | 3133194 |
| dopamine receptors in rabbit and rat eye: characterization and localization of da1 and da2 binding sites. | in vitro autoradiography is used to characterize and localize da1 and da2 receptors in rabbit and rat eyes. the bindings of 125i-sch 23982 (da1 antagonist) and of 125i-iodosulpride (da2 antagonist) to slide mounted tissue sections take place with characteristics expected of substances that recognize da1 and da2 receptors respectively. they are saturable, have high affinity and exhibit an appropriate pharmacology. the regional distribution of these receptors indicates that they are mainly present ... | 1989 | 2707038 |
| grimontia marina sp. nov., a marine bacterium isolated from the yellow sea. | a novel species belonging to the genus grimontia is described in this study. a gram-negative, chemoheterotrophic, obligately aerobic, catalase- and oxidase-positive, motile by a single polar flagellum, and rod-shaped bacterium, designated imcc5001(t), was isolated from surface seawater of the yellow sea. strain imcc5001(t) grew optimally at 30°c in the presence of 3.5% nacl. phylogenetic analysis based on 16s rrna gene sequences showed that the isolate was related most closely to grimontia holli ... | 2012 | 22367954 |
| chlorophyll a might structure a community of potentially pathogenic culturable vibrionaceae. insights from a one-year study of water and mussels surveyed on the french atlantic coast. | the present study focused on the isolation of culturable bacteria from mussels and sea water to identify vibrionaceae potentially pathogenic for humans. three sites located on the french atlantic coast were monitored monthly (twice each month during summer) for 1 year. environmental parameters were surveyed (water temperature, salinity, turbidity, chlorophyll a) and bacteria were detected by culture and identified by api 20e(®) systems (biomérieux) and pcr. a total of seven species were detected ... | 2010 | 23766015 |
| comparison of hemolysins of vibrio cholerae non-o1 and vibrio hollisae with thermostable direct hemolysin of vibrio parahaemolyticus. | hemolysin (vh-rtdh) produced by vibrio hollisae and hemolysin (nag-rtdh) produced by vibrio cholerae non-o1 were characterized and compared with hemolysin (vp-tdh) produced by vibrio parahaemolyticus. these three hemolysins are each composed of two subunits and have similar, but not identical, molecular weights. the amino acid compositions of vp-tdh and nag-rtdh are similar, but are different from that of vh-rtdh. the three hemolysins showed similar lethal toxicities to mice. the effects of temp ... | 1988 | 2466545 |
| properties of a hemolysin related to the thermostable direct hemolysin produced by a kanagawa phenomenon negative, clinical isolate of vibrio parahaemolyticus. | a hemolytic toxin (vp-trh) produced by a kanagawa phenomenon negative, clinical isolate of vibrio parahaemolyticus was further characterized. the purified vp-trh showed various biological activities, such as fluid accumulation in rabbit ileal loops, increase of rabbit skin vascular permeability, and cardiotoxicity on cultured myocardial cells, all of which are essentially similar to the activities found with thermostable direct hemolysin (vp-tdh), a pathogenic toxin produced by kanagawa phenomen ... | 1990 | 2118824 |
| implications for adult roles from differential styles of mother-infant bonding: an ethological study. | ethological observations of maternal and infant behaviors of nine vervet monkey pairs (cercopithecus aethiops sabaeus) showed the effects of differential styles of early maternal responsiveness on later infant competence. those infants receiving the least amount of maternal responsiveness and the most time-off the mother in the first 3 months of development were more socially competent at 6 months of age. the results are discussed within current ethological "attachment" theories. the detachment ... | 1979 | 105083 |
| immunochemical properties of oligosaccharide-protein conjugates with klebsiella-k2 specificity. i. specificity and crossreactivity of anti-conjugate versus anti-bacterial antibodies. | | 1979 | 86152 |
| blood transfusion and athletics. games people play. | | 2014 | 3974666 |
| endocrine and hemodynamic effects of antagonism of fentanyl-induced respiratory depression by nalbuphine. | endocrine and hemodynamic changes associated with the antagonism of fentanyl by nalbuphine have not been reported. therefore, the authors studied ten patients after anesthetic induction with thiopental, fentanyl, tracheal intubation aided by succinylcholine and maintenance with diazepam, pancuronium, n2o, and further doses of fentanyl. eight of the patients underwent cholecystectomy, one had a hysterectomy, and another had an abdominoplasty. after reversal of neuromuscular block at the conclusio ... | 2013 | 3578849 |
| medical numismatic notes. xvi. philemon holland, "the great translator". | | 2012 | 4604744 |
| combined evaluation of first pass radionuclide angiography and equilibrium radionuclide ventriculography in the diagnosis of coronary artery disease. ii. results during exercise. | results of 203 patients who underwent first pass radionuclide angiography (fp) and quantitative equilibrium radionuclide ventriculography (qernv) were stored in a data base system and evaluated statistically. eighty eight of these patients also underwent exercise equilibrium radionuclide ventriculography (e-qernv). in patients with coronary artery disease (cad) without previous myocardial infarction (mi), evaluation of global and regional ejection fraction (gef, ref) at rest revealed a poor sens ... | 2011 | 3034613 |
| immobilon in the otter. | | 2015 | 4450432 |
| [conservative treatment of injuries of the liver]. | | 2014 | 3911104 |
| the use of multivariate techniques in the study of skeletal populations. | | 2014 | 4313256 |
| effects of blocked versus random practice by mentally retarded subjects on learning a novel skill. | the purpose of this study was to assess the effects of different practice conditions on mentally retarded subjects learning a novel task. 20 subjects with a mean iq of 51.82 were randomly assigned to one of two practice conditions. the first group practiced at three different speeds (60, 30, and 15 rpm) on the pursuit rotor using a block arrangement in which each speed was practiced for five consecutive trials of 20 sec. duration before practicing at the next speed. the second group used random ... | 2010 | 2812988 |
| a senior elective in internal medicine. | | 1988 | 266287 |
| [microcirculation of the sulcus fluid in the crevicular epithelium and the epithelial attachment]. | | 1999 | 96395 |
| [newly designed ultraviolet light monochromator]. | | 2015 | 4542243 |
| fda cites veterinary responsibilities in reducing drug residues. | | 2007 | 2045321 |
| utilization of the intestine in surgery for major urological complications following gynaecological cancer. | | 2014 | 4119642 |
| [the activity of factor xiii in plasma of children with acute leucaemia (author's transl)]. | | 2015 | 4518176 |
| novel metagenome-derived, cold-adapted alkaline phospholipase with superior lipase activity as an intermediate between phospholipase and lipase. | a novel lipolytic enzyme was isolated from a metagenomic library obtained from tidal flat sediments on the korean west coast. its putative functional domain, designated mplag, showed the highest similarity to phospholipase a from grimontia hollisae cip 101886, though it was screened from an emulsified tricaprylin plate. phylogenetic analysis showed that mplag is far from family i.6 lipases, including staphylococcus hyicus lipase, a unique lipase which can hydrolyze phospholipids, and is more evo ... | 2012 | 22544255 |
| grimontia celer sp. nov., from sea water. | strain 96-237t, a gram-reaction-negative, curved- to spiral-shaped motile bacterium, isolated from coastal marine water, was found to be related to species of the genus grimontia by 16s rrna gene sequence comparison, sharing 98.3 % similarity to grimontia marina cect 8713tand 98.8 % to 'grimontiaindica' ak16. phenotypic analysis revealed that strain 96-237t is slightly halophilic, mesophilic and facultatively anaerobic, fermenting d-glucose, d-ribose, d-mannose, d-mannitol, maltose and sucrose. ... | 2016 | 27125858 |
| hydrogen peroxide causes vibrio vulnificus bacteriolysis accelerated by sulfonyl fluoride compounds. | induction of bacteriolysis of vibrio vulnificus cells by 10 mm hydrogen peroxide (h(2)o(2)) was analyzed. all vibrio species examined, except for vibrio hollisae, were lysed by 10 mm h(2)o(2). bacteriophage induction was not the cause of h(2)o(2)-induced bacteriolysis. autolysis is also known to cause bacteriolysis. vvps protein is a serine protease of v. vulnificus essential for autolysis. vvps mutant underwent h(2)o(2)-induced bacteriolysis in the same manner as the wild type. protease inhibit ... | 2015 | 26316164 |
| potential antitumor therapeutic application of grimontia hollisae thermostable direct hemolysin mutants. | we report on the preparation of a new type of immunotoxin by conjugation of an epidermal growth factor receptor (egfr)-binding peptide and an r46e mutation of thermostable direct hemolysin from grimontia hollisae, (gh-tdh(r) (46e) /eb). the hybrid immunotoxin was purified to homogeneity and showed a single band with slight slower mobility than that of gh-tdh(r) (46e) . cytotoxicity assay of gh-tdh(r) (46e) /eb on egfr highly, moderately, low, and non-expressed cells, a431, mda-mb-231, hela, and ... | 2015 | 25640743 |
| grimontia indica ak16(t), sp. nov., isolated from a seawater sample reports the presence of pathogenic genes similar to vibrio genus. | grimontia indica strain ak16(t) sp. nov. is the type strain of g. indica sp. nov. a new species within the genus grimontia. this strain, whose genome is described here, was isolated from seawater sample collected from southeast coast of palk bay, india. g. indica ak16(t) is a gram-negative, facultative aerobic rod shaped bacterium. there are only two other strains in the genus grimontia one of which, grimontia hollisae cip 101886(t), is a reported human pathogen isolated from human stool sample ... | 2014 | 24465608 |
| the thermostable direct hemolysin from grimontia hollisae causes acute hepatotoxicity in vitro and in vivo. | g. hollisae thermostable direct hemolysin (gh-tdh) is produced by most strains of g. hollisae. this toxin has been reported to be absorbed in the intestines in humans. secondary liver injury might be caused by venous return of the toxin through the portal system. we aimed to firstly analyze the in vitro and in vivo hepatotoxicity of gh-tdh. | 2013 | 23437095 |