| in vitro activity and stability against novel beta-lactamases of investigational beta-lactams (cefepime, cefpirome, flomoxef, sce2787 and piperacillin plus tazobactam) in comparison with established compounds (cefotaxime, latamoxef and piperacillin). | the therapeutic perspectives of flomoxef, sce 2787, cefpirome, cefepime, latamoxef, cefotaxime and of piperacillin plus tazobactam were comparatively evaluated by their in vitro activity against 1119 clinical isolates of 83 bacterial species. escherichia coli, klebsiella spp. enterobacter sakazakii, proteus spp. and shigella spp. were about equally susceptible to the cephalosporins (mic90: 0.06 to 0.5 mg/l), while the mic90 for piperacillin plus tazobactam was between 2 and 16 mg/l. enterobacter ... | 1991 | 1664418 |
| monoclonal antibodies to quebec strain (q17) of bovine rotavirus. | fourteen monoclonal antibodies (mabs) against "quebec" strain (q17) of bovine rotavirus were isolated and characterized. four were specific for viral protein vp7 and ten were specific for viral protein vp6. five different isotypes were represented by this group of antibodies. all of the anti-vp6 and none of the anti-vp7 antibodies were sensitive to the effects of periodate on their antigen. the antibodies could be separated into three groups based on their relative resistance to the dissociation ... | 1990 | 1698323 |
| effects of interleukin-2 (il-2) on human plasma lipid, lipoprotein, and c-reactive protein. | six patients with confirmed malignant disease received four consecutive weekly cycles of human recombinant interleukin-2 (il-2) 4 days/week, continuous iv. infusion, 3 x 10(6) u/m2/day. plasma cholesterol decreased a mean of 7% within 24 hours after il-2 infusion and decreased by 33% within 4 days. plasma cholesterol was significantly lower than baseline concentration by day 21 (-21%), and day 25 (-41%) was significantly lower than day 21. decreased plasma cholesterol was the result of decreased ... | 1990 | 1698421 |
| the structure of the biological repeating unit of the o-antigen of hafnia alvei o39. | on mild acid-catalysed degradation of the lipopolysaccharide from hafnia alvei o39 followed by gel filtration of sephadex g-50, the o-specific polysaccharide and three oligosaccharides were obtained, which represent the core substituted with 0-2 o-antigen repeating-units. on the basis of sugar and methylation analyses, 13c-n.m.r. data, solvolysis of the polysaccharide with anhydrous hydrogen fluoride, and computer-assisted 13c-n.m.r. analysis of the smith-degraded polysaccharide, it was conclude ... | 1990 | 1703456 |
| [the identification of lactose-negative enterobacteria]. | study of the morphologic and enzymatic characteristics of 10 strains of lactose-negative enterobacteria, sent to the l. a. tarasevich state institute for control and standardization as b. paracoli in order to determine their taxonomic status in accordance with the 1974 and 1984 bergey classifications has referred 9 of these strains to hafnia alvei. | 1990 | 1705611 |
| [diagnosis and therapy of viral hepatitis]. | | 1991 | 1713243 |
| o-specific polysaccharide of hafnia alvei lipopolysaccharide isolated from strain 1211. structural study using chemical methods, gas-liquid chromatography/mass spectrometry and nmr spectroscopy. | the hafnia alvei strain 1211 o-specific polysaccharide is composed of 3-amino-n-(d-3'-hydroxybutyryl)-3,6-dideoxy-d-galactose, n-acetyl-d-galactosamine, n-acetyl-d-glucosamine and d-glucose (1:1:2:2). on the basis of sugar and methylation analyses, smith degradation, and one- and two-dimensional 1h- and 13c-nmr spectroscopy, the polysaccharide was shown to be an o-acetylated polymer of the repeating hexasaccharide unit, ----2d(4-oac)fucp3nacyl beta 1----6dglcpnac alpha 1---- (dglcp beta 1----3)4 ... | 1991 | 1716201 |
| structural studies on hafnia alvei 114-60 o-antigen. | the structure of hafnia alvei 114-60 o-antigen has been established using sugar and methylation analyses as well as 1h-nmr spectroscopy. the results obtained proved that the repeating unit of hafnia alvei 114-60 o-antigen is identical to that of hafnia alvei atcc 13337 standard strain and has the following structure: [formula: see text] | 1990 | 1718234 |
| [hafnia alvei septicemia with shock and dic in an adult with postoperative lung cancer]. | in japan, we experienced the first case of hafnia alvei septicemia with shock and disseminated intravascular coagulation (dic) in an adult with postoperative lung cancer. a 63 year-old male, who had been followed up in our department since 1987, was admitted to our hospital with the complaints of fever, hemoptysis and dyspnea on june 25, 1989. after admission, he was treated with sulbactam/cefoperazone 4 g/day intravenously for suspicion of respiratory-tract infection. after antibiotic administr ... | 1991 | 1761901 |
| identification of salmonellae with the 4-methylumbelliferyl caprilate fluorescence test. | we have tested 750 salmonella strains and 130 strains of other species of the family enterbacteriaceae with the 4-methylumbelliferyl caprilate reagent (mucap) test. the mucap test is a fluorescence test for rapid identification of salmonella strains. the non-salmonella strains were strains sent for identification as suspected salmonella strains and thus have phenotypes similar to those of salmonella strains. all 748 tested salmonella strains of subgroups i, ii, iii, and iv were positive in the m ... | 1991 | 1774276 |
| the effect of exposure to impulse x rays on normal and epileptic activity in rabbit brain. | exposure to x rays (20 impulses of 4 hz frequency, total dose 0.6-1.1 mgy) increased the epileptic activity of a focal area, which was produced in the visual cortex of rabbit brain by freezing with liquid nitrogen, and by stimulating with flashes of light at frequencies of 5-6 hz. the number of seizure complexes during photostimulation for 5 s increased by 80% compared with the initial level, and this effect continued for 15 min. in control animals (with no epileptic foci), a decrease was observ ... | 1991 | 1797142 |
| the effect of exposure to impulse x rays on normal and epileptic activity in rabbit brain. | exposure to x rays (20 impulses of 4 hz frequency, total dose 0.6-1.1 mgy) increased the epileptic activity of a focal area, which was produced in the visual cortex of rabbit brain by freezing with liquid nitrogen, and by stimulating with flashes of light at frequencies of 5-6 hz. the number of seizure complexes during photostimulation for 5 s increased by 80% compared with the initial level, and this effect continued for 15 min. in control animals (with no epileptic foci), a decrease was observ ... | 1991 | 1797142 |
| serological characterization of lipopolysaccharides of hafnia alvei 1, 17, 31, 32, 38 and 39. | lipopolysaccharides of hafnia alvei strains 1, 17, 31, 32, 38 and 39 indicate strong cross-reactivity in passive hemagglutination. all lipopolysaccharides were examined by rocket immunoelectrophoresis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and immunoblotting. | 1991 | 1804049 |
| [clinical significance of the isolation of hafnia alvei from the feces of children with acute gastroenteritis]. | | 1991 | 1822161 |
| astrocytes: a possible primary site for colchicine-mediated neurotoxicity in the rat striatum. | ultrastructural changes in rat astrocytes including endfoot swelling and rupture of plasma membranes were seen as early as 1 h after injection of 6.0 micrograms colchicine (co) into the striatum. significant change in neuronal morphology was not seen by 24 h. astrocytes may therefore be primary targets for co-mediated damage, which then may have secondary neurotoxic consequences. | 1991 | 1828783 |
| mitochondrial damage by active oxygen species in vitro. | under in vitro conditions involving formation of active oxygen species, rat liver mitochondria were found to undergo swelling, peroxidative decomposition of lipids, and distinct disorganization of ultrastructure. supplementation with free radical scavengers such as superoxide dismutase (sod), methionine, histidine, and tryptophan accorded considerable protection to the organelle. a possible correlation between oxygen radicals, membrane integrity, and calcium functions is indicated. | 1991 | 1855671 |
| expression of a bacterial lysine decarboxylase gene and transport of the protein into chloroplasts of transgenic tobacco. | a possible approach for altering alkaloid biosynthesis in plants is the expression of genes encoding key enzymes of a pathway such as lysine decarboxylase (ldc) in transgenic plants. two strategies were followed here: one focused on expression of the gene in the cytoplasm, the other on subsequent targeting of the protein to the chloroplasts. the ldcgene from hafnia alvei was therefore (a) placed under the control of the 1' promoter of the bidirectional tr promoter from agrobacterium tumefaciens ... | 1991 | 1883998 |
| esp3i--a novel type iis restriction endonuclease from hafnia alvei that recognizes the sequence 5'-cgtctc(n)1/5-3'. | | 1991 | 1923776 |
| l-prolineaminopeptidase activity as a tool for identification and differentiation of serratia marcescens, serratia liquefaciens and hafnia alvei strains. | s. marcescens (316 strains), s. liquefaciens (10 strains) and hafnia alvei (20 strains), in contrast to 18 other enterobacteriaceae species, hydrolyzed l-proline-4-nitroanilide within 30 min at 37 degrees c. in this way, rapid identification of these species is possible. the substrate is applied in solution or in a paper disc. the substrate optimum of hafnia alvei strains proved to be 16 times lower than that of the serratia spp. application of a tenth of the substrate concentration necessary fo ... | 1991 | 1930558 |
| reactive modifications of the autonomous time structure in the human organism. | the spectrum of biological rhythms exhibits characteristic principles of biological time structure which also rule the functional behaviour. with increasing period lengths the rhythms become increasingly complex. in the long-wave section the rhythmic functions find their corresponding cycles in the environment, whereas the shorter waves represent only endogenous autonomous rhythms, which maintain an internal time order by means of frequency- and phase-coordination. under resting conditions and i ... | 1991 | 1932773 |
| etiologic role of hafnia alvei in human diarrheal illness. | | 1991 | 1937837 |
| induction of the alka gene of escherichia coli in gram-negative bacteria. | a broad-host-range plasmid containing a fusion of the alka and lacz genes of escherichia coli was introduced into various aerobic and facultative gram-negative bacteria--33 species belonging to 19 genera--to study the induction of expression of the alka gene by alkylating agents. the bacteria included species of the families enterobacteriaceae, pseudomonadaceae, rhizobiaceae, vibrionaceae, neisseriaceae, rhodospirillaceae, and azotobacteraceae. results obtained show that all bacteria tested, exc ... | 1991 | 1938974 |
| pathogenicity and antigenic components of excysted giardia lamblia isolated from patients in riyadh, saudi arabia. | giardia lamblia cysts were isolated from patients in riyadh, saudi arabia. cysts and trophozoites (from axenically excysted cysts) were given orally by gavage to mice to establish the pathogenicity of the riyadh isolate. there was no effect of varying the dose of administered parasite on parasite excretion or morbidity. a typical pathologic pattern of giardiasis was demonstrated by histologic methods and electron microscopy. antigenic components of the riyadh isolate were compared with the portl ... | 1991 | 1951852 |
| production and characterization of a monoclonal antibody specific for enterohemorrhagic escherichia coli of serotypes o157:h7 and o26:h11. | a monoclonal antibody (mab 4e8c12) specific for escherichia coli o157:h7 and o26:h11 was produced by immunizing balb/c mice with a rough strain of e. coli o157:h7. the antibody reacted strongly by a direct enzyme-linked immunosorbent assay with each of 36 strains of e. coli o157:h7. no cross-reactivity was observed with strains of salmonella spp., yersinia enterocolitica, shigella dysenteriae, proteus spp., escherichia hermanii, klebsiella pneumoniae, campylobacter jejuni, serratia marcescens, c ... | 1991 | 1993773 |
| hafnia alvei, a probable cause of diarrhea in humans. | hafnia alvei, a member of the family enterobacteriaceae, was the only species of bacteria cultured from the stool of a 9-month-old child who was admitted with a 3-day history of watery diarrhea. the isolated strain of h. alvei failed to produce heat-labile or heat-stable enterotoxins or shiga-like toxin i or ii and did not invade hela cells, nor did it cause keratoconjunctivitis (determined by the sereny test) in a guinea pig's eye. the strain, however, induced diarrhea in 8 of 12 adult rabbits ... | 1991 | 2004829 |
| structure of the o-specific, sialic acid containing polysaccharide chain and its linkage to the core region in lipopolysaccharide from hafnia alvei strain 2 as elucidated by chemical methods, gas-liquid chromatography/mass spectrometry, and 1h nmr spectroscopy. | mild acid hydrolysis of hafnia alvei strain 2 lipopolysaccharide released no o-specific polysaccharide but instead gave a monomeric octasaccharide repeating unit with n-acetylneuraminic acid as the reducing terminus. in addition, a dimer of the octasaccharide repeating unit, and also a decasaccharide composed of a fragment of the o-specific polysaccharide chain and the core region, were obtained in minute amounts. on the basis of the sugar and methylation analyses, periodate oxidation, and 1h nm ... | 1991 | 2036370 |
| identification of the bacterium associated with haemorrhagic septicaemia in rainbow trout as hafnia alvei. | an unidentified bacterium responsible for epizootic haemorrhagic septicaemic disease in rainbow trout was recently described. this organism exhibited some antigenic similarity to brucella abortus and to yersinia ruckeri and was presumed to belong to the family enterobacteriaceae. further biochemical characterization and dna relatedness studies showed that pathogenic fish strains are hafnia alvei. | 1990 | 2120748 |
| immunogenic and protective properties of outer membrane proteins of hafnia alvei. | outer membrane proteins (omp) extracted from antigenically distinct or related strains of hafnia alvei containing defined composition of major proteins proved to be immunogenic. intraperitoneal immunization of mice with a single dose of such preparations protected the animals against homologous and heterologous hafnia strains. the omp preparations were also found to induce protection with varying intensity against escherichia, proteus, shigella and salmonella. | 1990 | 2130804 |
| the normal microbial flora of the outer ear canal in healthy norwegian individuals. | the microbial flora of the outer ear canal was determined for 77 healthy individuals (m = 44, f = 33). no growth of any microbe was found in 5% of males and 15% of females. coagulase-negative staphylococci, dominated by staphylococcus epidermidis, were the commonest microbe group found (83% of persons sampled). staphylococcus aureus was found in 7% males and no females. diptheroids were cultured from 32% of the samples. the only gram-negative rod found was hafnia alvei in 4% of individuals. no v ... | 1990 | 2216049 |
| [typical nosocomial infection with an unusual cause: hafnia alvei. report of 2 cases and literature review]. | the route of infection and the course of two typical nosocomial infections are described in two patients infected with a rare gram-negative bacterium. both patients underwent cardiovascular surgery. they were placed close to each other in the intensive care unit for several days and suffered from pneumonia and from wound infection respectively. in both patients bacterial culture grew hafnia alvei. successful antibiotic treatment was achieved with netilmicin and imipenem. urinary tract, respirato ... | 1990 | 2218242 |
| purification of cellular retinoic acid-binding protein from human placenta. | | 1990 | 2292943 |
| endogenous endophthalmitis due to salmonella arizonae and hafnia alvei. | enteric pathogens rarely involve organs other than those of the gastrointestinal system. we have reported the case of a woman with rheumatoid arthritis who had endogenous endophthalmitis due to salmonella arizonae and hafnia alvei. the infection probably resulted from the use of snake powder as a food seasoner. after appropriate intravenous, intraocular, subconjunctival, topical, and oral antibiotic therapy based on laboratory susceptibility studies, the patient's condition clinically improved, ... | 1990 | 2346566 |
| kinetics of "p"-site-mediated inhibition of adenylyl cyclase and the requirements for substrate. | the kinetics of "p"-site-mediated inhibition of adenylyl cyclase was studied with the detergent-solubilized enzyme from rat brain. mn2(+)-activated adenylyl cyclase exhibited typical noncompetitive inhibition by 2'-d3'-amp or 2',5'-dideoxyadenosine (2',5'-ddado). however, enzyme that was preactivated with guanosine 5'-o-(3-thiotriphosphate) (gtp gamma s) or proteolytically with ninhibin (+ gtp gamma s) exhibited apparently uncompetitive inhibition with either 2'-d3'-amp or 2',5'-ddado and with e ... | 1990 | 2365688 |
| the sugar moiety of bovine s-antigen. | to obtain insight into the glycoprotein nature of s-antigen (s-ag), we have investigated the affinity of bovine s-ag for plant lectins, location of the sugar moiety within the molecule, and incorporation of radiolabelled mannose and glucosamine into s-ag. it was found (i) that only about 10% of purified s-ag was bound to concanavalin a (con a) and wheat germ agglutinin (wga) columns, (ii) that both concanavalin a and wheat germ agglutinin bound chymotryptic peptides derived from the c-terminal h ... | 1990 | 2384003 |
| hafnia alvei infection. | | 1990 | 2402660 |
| [a test for the identification of hafnia alvei bacteria by the color reaction with a complex of 5-nitro-8-hydroxyquinoline + iron ions]. | | 1986 | 2429042 |
| theophylline's inhibition of antigen-induced contraction of human parenchymal strips is independent of adenosine antagonism. | the effects of theophylline, and adenosine antagonist, and enprofylline, reported to lack adenosine antagonism, were studied using antigen-induced contraction of sensitized human lung parenchymal strips. cumulative addition of adenosine usually resulted in a small concentration-dependent contraction, which was antagonized by theophylline, 100 microm. antigen-induced contraction was reduced by pretreatment with adenosine, 10 microm, but not by adenosine addition after antigen challenge. dipyridam ... | 1987 | 2446888 |
| sequences that promote formation of catenated intertwines during termination of dna replication. | the normal sequence at which sv40 dna replication terminates (ter) is unusual in that it promotes formation of catenated intertwines when two converging replication forks enter to complete replication (weaver et al., 1985). here we show that yeast centromeric sequences also exhibit this phenomenon. cen3 caused accumulation of late replicating intermediates and catenated dimers in plasmids replicating in mammalian cells, but only when it was located in the termination region (180 degrees from ori ... | 1989 | 2542894 |
| o-specific polysaccharides of hafnia alvei lipopolysaccharides isolated from two serologically related strains: atcc 13337 and 1187. a serological and structural study using chemical methods, gas chromatography/mass spectrometry and nmr spectroscopy at 500 mhz. | the o-specific polysaccharides of hafnia alvei atcc 13337 standard strain and 1187 strain have been isolated and characterized. by means of 1h-nmr spectroscopy, methylation analysis and periodate oxidation, the repeating unit of the polysaccharides could be allocated the respective structures. (formula; see text) where acyl = d-3-hydroxybutyryl, and 3-o-acetylation was to about 66%. the structural similarity of the polysaccharides was confirmed in the serological study; their epitopes were deter ... | 1989 | 2606108 |
| alkaline phosphatase in cholestatic and cirrhotic rats. a biochemical and histochemical study. | alkaline phosphatase (alp) was studied by enzyme histochemical methods and by biochemical quantitations in rat livers with chronic bile duct obstruction and experimental cirrhosis. the most evident alp increase was histochemically found in portal tracts of rats with bile duct obstruction and localized to the walls of proliferating blood vessels. furthermore, a slight canalicular membrane enzyme increase was histochemically found in both groups, most evident in cirrhosis, whereas the biochemical ... | 1989 | 2612454 |
| alkaline phosphatase in cholestatic and cirrhotic rats. a biochemical and histochemical study. | alkaline phosphatase (alp) was studied by enzyme histochemical methods and by biochemical quantitations in rat livers with chronic bile duct obstruction and experimental cirrhosis. the most evident alp increase was histochemically found in portal tracts of rats with bile duct obstruction and localized to the walls of proliferating blood vessels. furthermore, a slight canalicular membrane enzyme increase was histochemically found in both groups, most evident in cirrhosis, whereas the biochemical ... | 1989 | 2612454 |
| tissue distribution of a novel neurotensin-degrading metallopeptidase. an immunological approach using monospecific polyclonal antibodies. | a monospecific polyclonal antiserum was raised against a recently purified rat brain neurotensin-degrading metallopeptidase. the purified igg fraction immunoprecipitated the peptidase and inhibited its proteolytic activity. western blot analyses revealed that the immune fraction recognizes only one protein in rat brain homogenates, and this corresponds closely to the purified enzyme. the igg displayed a restricted specificity towards the peptidase from murine origin. in the rat, the neurotensin- ... | 1989 | 2649078 |
| oviducal storage of spermatozoa in the turkey: its relevance to artificial insemination technology. | 1. this paper addresses questions concerning the role of oviducal sperm selection and storage in the relative inefficiency of turkey reproduction. 2. before the onset of egg production, the turkey has the biological potential to store spermatozoa in its oviduct for periods up to 5 weeks; after the onset of production fewer spermatozoa are retained by the oviduct. 3. a better understanding of the biological mechanisms which regulate sperm selection and storage in the turkey oviduct is necessary t ... | 1989 | 2670069 |
| [insulin and glucocorticoid binding by blood cell receptors after hydrocortisone administration in rabbits]. | repeated i.v. administration of hydrocortisone (10 mg/kg) revealed an increase in the resistance against insulin although endogenous corticosterone was decreased in 33 male rabbits. the insulin- and dexamethasone-binding receptors of erythrocytes and mononuclear leucocytes. changed after 3-7 hydrocortisone injections, the binding increasing for insulin and diminishing for dexamethasone. | 1989 | 2680632 |
| quantitation of antegrade and retrograde blood flow in the human aorta by magnetic resonance velocity mapping. | magnetic resonance velocity mapping was used in 24 normal subjects to study two-dimensional velocity profiles in the proximal and mid-ascending aorta, and to quantify both forward and reverse flow. the aortic flow measurements were validated by comparison with left ventricular stroke volume in all subjects and by comparison with pulmonary flow measurements in 12. agreement was good with standard errors of the estimate of 7.8 and 7.1 ml, and correlation coefficients of 0.93 and 0.95, respectively ... | 1989 | 2729051 |
| effect of paf on parasympathetic contraction of canine airways. | platelet-activating factor (paf) increases the bronchoconstrictor response of mammalian airways to cholinergic agonists and is thus implicated as a potential mediator of airway hyperreactivity. this study further defines the nature of the increase in airway responsiveness induced by paf. we employed an in situ canine tracheal preparation, which allows differentiation of the effects paf has on airway smooth muscle contraction from confounding effects it has on inducing airway edema and secretions ... | 1989 | 2745324 |
| volatile compounds associated with microbial growth on normal and high ph beef stored at chill temperatures. | volatile compounds produced by pseudomonas fragi and mixed, natural floras on beef of normal ph (5.5-5.8; glucose greater than 1500 micrograms/g) and high ph (6.3-6.8; glucose less than 10 micrograms/g) included a range of alkyl esters and a number of sulphur-containing compounds including dimethylsulphide but not hydrogen sulphide. production of the last was a property common to the other gram-negative organisms tested viz. hafnia alvei, enterobacter agglomerans, serratia liquefaciens, alteromo ... | 1989 | 2753835 |
| perioral anesthesia disrupts maternal behavior during early lactation in long-evans rats. | the role of perioral somatosensory stimulation from pups in maternal behavior was investigated in lactating long-evans rats (rattus norvegicus) during early lactation by local anesthesia of the mystacial pads with lidocaine, which blocks conduction in the infraorbital nerve. it was concluded that (1) acute perioral anesthesia markedly inhibited pup retrieval when the subjects had no formal pretreatment retrieval experience. further, these dams displayed little tolerance to the lidocaine treatmen ... | 1989 | 2757582 |
| reduction of lipid peroxidation in reperfused isolated rabbit hearts by diltiazem. | the calcium-channel inhibiting agent, diltiazem, has been shown to enhance salvage of reperfused myocardium independent of effects on coronary blood flow or myocardial work. because lipid peroxidation may be a mediator of reperfusion injury and modifiable by calcium-sensitive pathways, we evaluated the effects of diltiazem on the formation of malondialdehyde (mda), a product of lipid peroxidation, in isolated rabbit hearts perfused with buffer under control conditions or after 60 minutes of isch ... | 1989 | 2766494 |
| effects of glucagon on systemic and splanchnic circulation in conscious rats with biliary cirrhosis. | to elucidate the relationship between the haemodynamic changes and glucagon in cirrhosis, we infused physiologic and supraphysiologic doses of this hormone in conscious rats with portal hypertension due to biliary cirrhosis. cardiac output and splanchnic organ blood flows were measured by the radioactive microsphere method before and 30 min after glucagon infusion at doses of 2, 5 and 10 ng/min. serum glucagon increased from a basal level of 92 +/- 17 pg/ml (mean +/- s.e.) to 399 +/- 89, 1151 +/ ... | 1989 | 2768797 |
| [cardiovascular risk factors in children and adolescents in fuenlabrada: objectives, features of the population, design, participation, equipment, laboratory technics, and quality control]. | fuenlabrada is at the present time the most industrialized town placed at the south are of madrid. this population has some peculiar attractive characteristics from the epidemiologic point of view. perhaps the most important is that the majority of its inhabitants are immigrant from other depressed rural areas of the country, with a low socio-economic and cultural level. the new conditions of life could have changed the life expectation and the causes of morbidity and mortality of this populatio ... | 1989 | 2772364 |
| protection induced against acute bronchitis--the use of human and rat models to determine mechanisms of action of oral immunization with haemophilus influenzae. | | 1989 | 2786462 |
| on the estimation of pulmonary capillary pressure from arterial occlusion. | we have attempted to evaluate some approaches for estimating pulmonary capillary pressure from the transient pressure data obtained from the tip of the swanz-ganz catheter after inflation of the balloon. to this end experiments were carried out in anesthetized dogs in which pulmonary vasoconstriction was induced by infusion of histamine, serotonin, or norepinephrine or by hypoxia. in a group of closed chest dogs, the time course for the fall in the catheter tip pressure toward the wedge pressure ... | 1989 | 2817586 |
| response of human chorionic gonadotrophin to luteinizing hormone-releasing hormone stimulation in the culture media of normal human placenta, choriocarcinoma cell lines, and in the serum of patients with gestational trophoblastic disease. | in vitro and in vivo responses to luteinizing hormone-releasing hormone (lhrh) stimulation of human chorionic gonadotrophin (hcg) production were evaluated. we cultured placental tissues of ten weeks and term pregnancy, choriocarcinoma tissues, and monolayers of the bewo cell line, and added serial dilutions of lhrh (1, 5 and 10 micrograms) to the media for five to seven days. in in vivo experiments, 100 micrograms lhrh was intravenously administered to 20 normally cycling women (control group), ... | 1987 | 2821528 |
| response of human chorionic gonadotrophin to luteinizing hormone-releasing hormone stimulation in the culture media of normal human placenta, choriocarcinoma cell lines, and in the serum of patients with gestational trophoblastic disease. | in vitro and in vivo responses to luteinizing hormone-releasing hormone (lhrh) stimulation of human chorionic gonadotrophin (hcg) production were evaluated. we cultured placental tissues of ten weeks and term pregnancy, choriocarcinoma tissues, and monolayers of the bewo cell line, and added serial dilutions of lhrh (1, 5 and 10 micrograms) to the media for five to seven days. in in vivo experiments, 100 micrograms lhrh was intravenously administered to 20 normally cycling women (control group), ... | 1987 | 2821528 |
| selective induction of chromosomal gene expression by human cytomegalovirus. | during permissive infection, human cytomegalovirus (hcmv) increases the activities of certain cellular enzymes. in this report, we show that induction of these cellular genes occurs at the level of rna. the abundance of several additional growth-regulated cellular transcripts in hcmv-infected human foreskin fibroblasts (hff) was also compared to that in serum-stimulated hff cells. hcmv infection of hff cells resulted in increased abundance of ornithine decarboxylase, thymidine kinase, heat-shock ... | 1988 | 2842946 |
| biological and pathological consequences of feline infectious peritonitis virus infection in the cheetah. | an epizootic of feline infectious peritonitis in a captive cheetah population during 1982-1983 served to focus attention on the susceptibility of the cheetah (acinoyx jubatus) to infectious disease. subsequent observations based upon seroepidemiological surveys and electron microscopy of fecal material verified that cheetahs were indeed capable of being infected by coronaviruses, which were antigenically related to coronaviruses affecting domestic cats, i.e. feline infectious peritonitis virus/f ... | 1988 | 2849387 |
| a pulsed epr study of redox-dependent hyperfine interactions for the nickel centre of desulfovibrio gigas hydrogenase. | the nickel centre of hydrogenase from desulfovibrio gigas was studied by electron spin echo envelope modulation (eseem) spectroscopy in the oxidized, unready (ni-a) and h2-reduced active (ni-c) states, both in h2o and 2h2o solutions. fourier transforms of the 3-pulse eseem, taken at 8.7 ghz, for ni-a and ni-c in h2o contained similar peaks with narrow linewidths at frequencies of 0.4, 1.2 and 1.6 mhz, and a broader peak centered at 4.5 mhz. at 11.6 ghz, the low frequency components showed small ... | 1988 | 2849556 |
| peroxisomal oxidases and suggestions for the mechanism of action of insulin and other hormones. | | 1985 | 2863924 |
| hypotensive effects after topical and intra-arterial administration of alpha-adrenergic agonists in isolated perfused rabbit eyes. | the influence of nonselective and selective alpha-adrenergic agonists on intraocular pressure (iop) was studied in isolated arterially perfused rabbit eyes. drugs were given intra-arterially or topically. with either route of administration, no initial hypertensive phase was observed after l-epinephrine, l-norepinephrine, l-phenylephrine (alpha 1), or methoxamine (alpha 1), but there was an immediate ocular hypotensive effect. the alpha 2-agonist b-ht 920 caused a nonsignificant 1 mm hg fall in ... | 1985 | 2866480 |
| experimental approach of treatment of cerebral ischemia by a combination of raubasine and almitrine. | post-oligemic syndrome in rat results from transient reduction of cerebral blood flow obtained by bilateral carotid ligation for 60 min and mild systemic hypotension induced by s.c. injection of sodium nitroprusside. over an acute phase of 3 days the syndrome consisted in a marked neurological deficit evidenced by a decrease in scores obtained by rats in "visual placing" test, a loss in learning ability substantiated by a decrease in the ability of rats to integrate and remember a passive avoida ... | 1987 | 2887170 |
| acetyl-homocysteine-thiolactone-induced increase of superoxide dismutase counteracts the effect of subdiabetogenic doses of streptozocin. | acetyl-homocysteine-thiolactone (cyt) is an organic thio compound that exerts free radical scavenger activity and increases superoxide dismutase (sod) activity. administration of 32 mg cyt/kg body wt/day/30 days in rats increased sod activity in erythrocytes by 126%, and in pancreatic islets by 202%. treatment affected only the cu-zn fraction of the enzyme. transmission electron microscope observations showed that the damage to the pancreatic beta cells induced by single or multiple subdiabetoge ... | 1986 | 2937674 |
| [anopheles breeding in the municipal reservoir of the city of ustinov]. | | 1986 | 2945087 |
| further differentiation of enterobacteriaceae by means of siderophore-pattern analysis. | by means of a combination of 5 siderophore bioassays using several indicator strains, genera, species and subspecies of enterobacteriaceae can be further differentiated. enterobactin, aerobactin and other siderophores produced can be detected. each strain shows specific pattern which we called siderophore-pattern. it is easy to separate morganella, proteus, providencia, yersinia strains from the genera salmonella, shigella, escherichia coli, enterobacter, citrobacter, klebsiella, serratia. enter ... | 1988 | 2970195 |
| opioid influence on some aspects of stereotyped behavior induced by repeated amphetamine treatment. | rats were administered repeated ip injections of dl-amphetamine (amph) according to a chronic escalating dose schedule (three doses per 24 hr, for four days, two days or one day). animals treated for four days exhibited a diminished oral stereotypy in response to a challenge of 12 mg/kg amph or 2 mg/kg sc apomorphine (apo), 72 hr after withdrawal. pretreatment with 2 mg/kg ip naloxone (nal) during the period of chronic amph administration prevented the reduction in oral stereotypy induced by amp ... | 1988 | 2976168 |
| regulation of the glucose phosphotransferase system in brochothrix thermosphacta by membrane energization. | uptake of 2-deoxyglucose, alpha-methylglucopyranoside, and glucose into intact cells of brochothrix thermosphacta (formerly microbacterium thermosphactum, atcc 11509) was stimulated by kcn or cccp. the glucose analogs were recovered almost totally as the sugar phosphates. membrane vesicles were isolated from protoplasts and shown to be right side out by freeze fracturing and by using atpase as a marker for the cytoplasmic membrane surface. uptake of glucose into vesicles was dependent on the pre ... | 1985 | 2995314 |
| anti-ischemic actions of a new thromboxane receptor antagonist during acute myocardial ischemia in cats. | thromboxane a2 (txa2) production increases significantly during acute myocardial ischemia. since txa2 induces platelet aggregation, coronary vasoconstriction, and has a direct cytolytic effect, thromboxane receptor antagonism would be expected to be beneficial in acute myocardial ischemia. thirty minutes after ligation of the left anterior descending coronary artery (lad) in anesthetized cats, the txa2 receptor antagonist bm-13,177 or its vehicle was given as a bolus injection at 20 mg/kg, follo ... | 1985 | 3000159 |
| identification of the stable free radical tyrosine residue in ribonucleotide reductase. | the small subunit of iron-dependent ribonucleotide reductases contains a stable organic free radical, which is essential for enzyme activity and which is localized to a tyrosine residue. tyrosine-122 in the b2 subunit of escherichia coli ribonucleotide reductase has been changed into a phenylalanine. the mutation was introduced with oligonucleotide-directed mutagenesis in an m13 recombinant and verified by dna sequencing. purified native and mutant b2 protein were found to have the same size, ir ... | 1986 | 3019680 |
| identification of the stable free radical tyrosine residue in ribonucleotide reductase. | the small subunit of iron-dependent ribonucleotide reductases contains a stable organic free radical, which is essential for enzyme activity and which is localized to a tyrosine residue. tyrosine-122 in the b2 subunit of escherichia coli ribonucleotide reductase has been changed into a phenylalanine. the mutation was introduced with oligonucleotide-directed mutagenesis in an m13 recombinant and verified by dna sequencing. purified native and mutant b2 protein were found to have the same size, ir ... | 1986 | 3019680 |
| evidence that a human soluble beta-galactoside-binding lectin is encoded by a family of genes. | two cdna clones were isolated by immunoscreening a human hepatoma cdna library with an antiserum that bound specifically to a human soluble beta-galactoside-binding lectin with mr of approximately 14,000. the deduced amino acid sequences of the inserts of these two clones show considerable homology with each other, the sequence of chicken skin beta-galactoside-binding lectin, and eight peptides derived from purified human lung lectin of mr approximately 14,000. however, the sequence differences ... | 1986 | 3020551 |
| evidence that a human soluble beta-galactoside-binding lectin is encoded by a family of genes. | two cdna clones were isolated by immunoscreening a human hepatoma cdna library with an antiserum that bound specifically to a human soluble beta-galactoside-binding lectin with mr of approximately 14,000. the deduced amino acid sequences of the inserts of these two clones show considerable homology with each other, the sequence of chicken skin beta-galactoside-binding lectin, and eight peptides derived from purified human lung lectin of mr approximately 14,000. however, the sequence differences ... | 1986 | 3020551 |
| [effects of 5 derivatives of 3-azabicyclo [3,3,1]nonanes on isolated guinea pig ileum myenteric plexus-longitudinal muscle]. | | 1985 | 3020868 |
| heterologous protection against rotavirus-induced disease in gnotobiotic piglets. | administration per os of 2 x 10(6) fluorescent cell-forming units of a human serotype 3 rotavirus (rv-3) protected all of nine gnotobiotic piglets against severe diarrheal disease when they were challenged 10 to 14 days later with 8 x 10(3) fluorescent cell-forming units of virulent wild-type porcine rotavirus (at/76). the porcine virus was similar antigenically to porcine prototype strain osu, previously described as antigenically distinct from all four recognized human serotypes. administratio ... | 1986 | 3023436 |
| hafnia alvei septicemia in an infant with necrotizing enterocolitis. | hafnia alvei is an infrequently reported pathogen in children, and its isolation in a newborn is even more unusual. this organism is rarely associated with invasive disease. this article reports the first case of a neonate with necrotizing enterocolitis and subsequent ileal perforation who had h. alvei isolated from both blood and stool. | 1988 | 3057140 |
| [biochemical typing of enterobacter isolated from several clinical materials]. | a total of 74 enterobacter species have been isolated from the patients applying to the department of microbiology, university of ankara, studying several biochemical test systems their strains have been found. 37 of these enterobacter species have been found to be enterobacter cloacae, 10 enterobacter agglomerans, 13 enterobacter aerogenes, 3 enterobacter hafniae (hafnia alvei), 1 enterobacter sakazakii. 2 of the strains couldn't be classified. in conclusion most of the strains were found to be ... | 1988 | 3078792 |
| modulation of complement fixation and the phlogistic capacity of group a, b, and d streptococci by human lysozyme acting on their cell walls. | streptococci and streptococcal cell wall fragments induce arthritis in rats, with the severity and duration depending on the capacity of the cells or cell fragments to resist degradation by tissue enzymes. their phlogogenic effects are apparently related to their ability to activate the alternate complement pathway (acp). the in vitro activation of the acp by lysozyme-treated cells and cell walls of group a, b, and d streptococci suggests that both rat and human lysozyme can modulate this activi ... | 1986 | 3086232 |
| in vitro activity of aztreonam against gram negative bacteria from clinical specimens and its comparison with other commonly used antibiotics. | a total of 755 gram negative bacteria isolated from clinical specimens were tested against aztreonam by the disc agar diffusion test. the strains of bacteria used in this study consisted of escherichia coli (314), enterobacter aerogenes (30), e. agglomerans (7), e. cloacae, (39), citrobacter diversus (9), c. freundii (13), hafnia alvei (3), acinetobacter calcoaceticus (10), klebsiella oxytoca (6), k. ozaenae (5), k. pneumoniae (107), morganella morganii (3), moraxella sp. (10), pasteurella multo ... | 1986 | 3088346 |
| galactosyltransferase: multiple forms in serum of normal and hepatoma mc-29 bearing chickens and from liver and hepatoma microsomal and plasma membrane preparations. | the multiple forms of galactosyltransferase in chicken serum and in microsomal and plasma membrane preparations from liver and viral induced hepatoma mc-29 have been studied by isoelectric focussing. an elevation of the hepatoma plasma membrane enzyme activity was described and in the pattern of the multiple forms of the enzyme two forms were found (pi-5.34 and 8.22) which were similar to those described in the serum of hepatoma bearing chickens (pi-5.36 and 8.24). a conclusion is drawn that the ... | 1986 | 3102037 |
| night-day differences in the ultradian rhythmicity of plasma renin activity. | during night-sleep, plasma renin activity displays periodic oscillations which are closely related to the alternation of rem-nrem sleep. to see whether this nocturnal rhythm persisted during the day-time, plasma renin activity was measured every 10 min for 24 hours in 4 human volunteers and in 4 others over a 10-h day-time period. to avoid the influence of repeated food intake which stimulates renin release, the subjects were on continuous enteral nutrition. spectral analyses of the data reveale ... | 1987 | 3108599 |
| lactate dehydrogenase and malate dehydrogenase of sertoli cells in rats. | lactate-dehydrogenase (ldh) and malate-dehydrogenase (mdh) activities and isozyme patterns of maturing sertoli cells were investigated in cultured cells obtained from 2-5-week-old rats. both enzymes, and more strikingly ldh, increased between 2 and 3 weeks of age. ldh isozyme complement included all five isozymes and, in the more mature rats, had a shift toward the isoforms rich in subunit. these data are consistent with an increasing production of lactate/pyruvate and with a finer regulation of ... | 1987 | 3122678 |
| effects of rev 5901, a 5-lipoxygenase inhibitor and leukotriene antagonist, on pulmonary responses to platelet activating factor in the guinea-pig. | 1. the effects of rev 5901 on the platelet activating factor (paf)-induced (a) bronchoconstriction, (b) contraction of lung parenchymal strips and (c) increased airways responsiveness to histamine, were assessed in the guinea-pig. rev 5901 is a 5-lipoxygenase inhibitor and competitive peptidoleukotriene antagonist which does not inhibit multiple forms of phosphodiesterase. 2. in urethane/pentobarbitone anaesthetized animals, rev 5901 (10 or 30 mg kg-1, i.v.) substantially inhibited the bronchoco ... | 1988 | 3145088 |
| a conductance medium to distinguish between salmonella and citrobacter spp. | published methods for detecting salmonella by conductance methods yield some citrobacter spp. as false positives. a medium is described which, when used in conjunction with selenite-cystine/trimethylamine oxide/mannitol (sc/t/m) resolves this difficulty. it is based on the ability of salmonella to decarboxylate lysine in the presence of selenite (4 g/l), after pre-enrichment in buffered peptone water supplemented with glucose and lysine for 7-17 h. salmonella produced a conductance change of 250 ... | 1988 | 3152806 |
| acute circulatory effects of doxorubicin in the conscious dog. | experimental work on the mechanisms of acute doxorubicin-induced cardiotoxicity may contribute to a better understanding of the clinical problem of cardiac failure after treatment with anthracycline derivatives. we studied aortic pressure and heart rate continuously for 1 h following a bolus injection of doxorubicin (1 mg/kg) in 7 dogs. in contrast with previous studies in intact animals, no anesthesia was used in order to eliminate possible interactions of doxorubicin with other drugs. one minu ... | 1986 | 3153244 |
| rodorubicin, a new tetraglycosidic anthracycline. | rodorubicin is a new tetraglycosidic anthracycline, which was detected because of its activity against human tumors in a human tumor based screening system. rodorubicin is not active in the typical animal transplantation tumors and might therefore be a new leading structure with preferential activity against slow proliferating human tumors. in spite the fact that rodorubicin is a chemical anthracycline, the drug has an untypical spectrum of activity and toxicity when compared to standard anthrac ... | 1988 | 3165634 |
| [specific binding of phenol-containing antioxidant with plasma membranes of cells and its inhibition by various biologically active substances]. | | 1988 | 3168734 |
| [psychosocial consequences of hiv infection]. | a questionnaire survey of 103 patients of the paris pitié-salpêtrière hospital measures the changes in family life, work relations and in sexual behaviour occurring after an hiv infection diagnosis. most patients choose to keep their diagnosis secret, an attitude that places them into a double bind situation. this silence, chosen as a mean of self-protection, prevents patients from making themselves understood and from mobilising the material and psychological help they need. | 1988 | 3187146 |
| concerning experimental animal tumors associated with silicone gel. dow corning corporation. | | 1988 | 3191656 |
| crystallization and purification of the enzyme anthranilate phosphoribosyl transferase. | anthranilate phosphoribosyl transferase from the bacterium hafnia alvei has been crystallized. this enzyme is one of a small number that constitute the biosynthetic pathway for tryptophan. large cubic crystals were grown at 4 degrees c by dialyzing away the glycerol from a protein solution that included ammonium sulfate, polyethylene glycol and glycerol. the crystals were much more temperature stable and resistant to x-ray deterioration than a previous, similar crystal form that had included gly ... | 1988 | 3199444 |
| [the aerobic intestinal flora of the microchiropteran bat chaerephon pumila in madagascar]. | from stools of 88 malagasy insect eater bats chaerephon pumila, a large amount of bacterial strains were isolated. twenty different species were recognized, most of them belonged to enterobacteriaceae family. eight strains were identified as salmonella enterica subsp. enterica o48: -; 1.5, despite many atypical characters. strains of salmonella enteritidis, koserella trabulsii, kluyvera sp., odc negative serratia marcescens, atypical hafnia alvei... were also identified. | 1988 | 3240566 |
| outer membrane protein composition of hafnia alvei. | the outer membrane proteins (omp) obtained from 33 strains of hafnia of different origin, were analysed by sds-polyacrylamide gel electrophoresis. about 21 protein bands could be distinguished in omp of each strain. the major proteins of the outer membrane were of apparent molecular weight 34.000 (34k), 37.000 (37k), 39.000 (39k) and 41.000 (41k). on the basis of the electrophoregrams obtained, four groups of strains possessing different composition of major omp were distinguished: (1) 34k and 3 ... | 1988 | 3254120 |
| hafnia alvei lipopolysaccharides: isolation, sugar composition and sds-page analysis. | lipopolysaccharides (lps) of 33 strains of hafnia alvei were isolated and purified. lps content of the dry bacterial mass ranged from 1.2 to 4.5%. all examined lipopolysaccharides contained glucose, glucosamine, heptose, 3-deoxy-octulosonic acid and often galactose. rhamnose, mannose, galactosamine, mannosamine and unidentified amino sugars were found in some h. alvei strains. sialic acid was present in lps of one strain. d-3-hydroxybutyryl groups also were identified in lipopolysaccharides of 5 ... | 1988 | 3273465 |
| [medico-genetic studies of human provisional organs]. | | 1987 | 3318529 |
| inborn errors of biotin metabolism. | the important role of biotin in human physiology has been highlighted by the recognition of two newly discovered human inborn errors of the metabolism of biotin. the molecular defect in the neonatal-onset disease is in the enzyme holocarboxylase synthetase. the defect in the later infantile-onset disease is in the enzyme biotinidase. both disorders present with impressive clinical manifestations involving the skin and hair. in the neonatal disease, alopecia totalis is associated with a bright re ... | 1987 | 3318710 |
| novel oral vaccines: the effectiveness of cloned gene products on inducing secretory immune responses. | | 1987 | 3321939 |
| the elastic anisotropy of bone. | in modeling the anisotropic properties of hydroxyapatite (hap), katz found that two kinds of phenomenological relationships held among the elastic stiffness coefficients. firstly, there are three linear combinations--(c11 + c22 + c33), (c44 + c55 + c66), (c12 + c13 + c23)--which arise naturally when computing the isotropic averages of anisotropic crystal systems over all possible spatial orientations. secondly, the degree of elastic anisotropy in such crystal systems is characterized by two spec ... | 1987 | 3323198 |
| sch 37137, a novel antifungal compound produced by a micromonospora sp. taxonomy, fermentation, isolation, structure and biological properties. | a new antifungal compound, sch 37137, was isolated from the cultured broth of a micromonospora sp., scc 1792. sch 37137 is structurally related to a 19009, a dipeptide previously discovered from a streptomyces sp. the compound was weakly active against species of candida and dermatophytes (mean mics greater than or equal to 128 micrograms/ml) in sabouraud dextrose, yeast-nitrogen and modified eagles minimum essential media; however activity against candida sp. (mean mics greater than or equal to ... | 1988 | 3346184 |
| [pulmonary nocardiosis as a presenting form of acquired immunodeficiency syndrome]. | | 1988 | 3347124 |
| trichosanthin, alpha-momorcharin and beta-momorcharin: identity of abortifacient and ribosome-inactivating proteins. | the abortifacient proteins trichosanthin, alpha-momorcharin and beta-momorcharin at nm concentrations inhibit cell-free protein synthesis. the momorcharins and the ribosome-inactivating proteins isolated from momordica charantia seeds cross-react with the respective antisera. the ribosome-inactivating proteins saporins, pokeweed antiviral protein (pap) and, to a lesser extent, gelonin have abortifacient activity on pregnant mice. | 1988 | 3372132 |
| experimental evaluation of the mitroflow pericardial heart valve prosthesis. part ii. pathologic examination. | the results of the morphologic and histopathologic examination of 37 bovine pericardial heart valve prostheses that were retrieved after experimental implantation in chacma baboons for periods of one to twelve months are presented in this study. the implanted prostheses consisted of 33 mitroflow valves, size 21 mm, that belonged to four different groups according to the method of preparation of the pericardium (process i to iv) and 4 commercially available ionescu-shiley valves, size 19 mm. all ... | 1988 | 3421507 |
| two-hour miniaturized system for detection of enteropathogenic bacteria in stool cultures. | a miniaturized 2-h system for detection of lactose-negative potentially enteropathogenic bacteria on the basis of nine enzymatic tests was evaluated. the system was challenged with 210 strains of lactose-negative and lactose-positive species grown on macconkey agar. specific and constant patterns were found for salmonella, shigella a-c, yersinia enterocolitica, aeromonas spp. and vibrio cholerae. shigella sonnei and hafnia alvei had an identical pattern, likewise plesiomonas shigelloides, haloph ... | 1987 | 3440458 |