| bacterial peptide methionine sulphoxide reductase: co-induction with glutathione s-transferase during chemical stress conditions. | peptide methionine sulphoxide reductase (msra; ec 1.8.4.6) is a ubiquitous enzyme catalysing the reduction of methionine sulphoxide to methionine in proteins, while the glutathione s-transferases (gsts) are a major family of detoxification enzymes. a gene homologous to msra was identified in a chromosomal fragment from the bacterium ochrobactrum anthropi, and this gene is located just downstream of a gst gene identified previously (oagst) [favaloro, tamburro, angelucci, de luca, melino, di ilio ... | 2001 | 11736659 |
| ochrobactrum anthropi bacteremia in pediatric oncology patients. | ochrobactrum anthropi is an emerging pathogen in immunocompromised hosts, particularly in patients with indwelling catheters. we report the characteristics of 14 o. anthropi bacteremic episodes in 11 children with hickman-type central catheters. children presented with fever and nonspecific clinical manifestations. bacteremia was successfully treated with antibiotics, but catheter removal was necessary to achieve cure in four cases. | 2002 | 11791106 |
| ochrobactrum anthropi pseudobacteraemia. | | 2001 | 11798265 |
| ochrobactrum anthropi bacteremia: case report and review of the literature. | | 1999 | 11851711 |
| infection caused by ochrobactrum anthropi. | | 1996 | 11866765 |
| bac library of t. pallidum dna in e. coli. | treponema pallidum subspecies pallidum (nichols) chromosomal dna was used to construct a large insert bacterial artificial chromosome (bac) library in escherichia coli dh10b using the pbelobac11 cloning vector; 678 individual insert termini of 339 bac clones (13.9 x coverage) were sequenced and the cloned chromosomal region in each clone was determined by comparison to the genomic sequence. a single 15.6-kb region of the t. pallidum chromosome was missing in the bac library, between bp 248727 an ... | 2002 | 11875041 |
| recombinant ochrobactrum anthropi expressing brucella abortus cu,zn superoxide dismutase protects mice against b. abortus infection only after switching of immune responses to th1 type. | the members of the genus brucella are gram-negative, facultatively intracellular bacterial pathogens that cause brucellosis in many animal species and humans. although live, attenuated vaccines are available to protect several animal species from the disease, there is no safe and effective vaccine for human use. here we report that a bacterium that is closely related to brucella species, ochrobactrum anthropi, can be used as a vaccine vector for the delivery of brucella antigens to mice, leading ... | 2002 | 11953393 |
| origin and evolution of the ampc beta-lactamases of citrobacter freundii. | to determine whether the widespread clinical use of beta-lactams has been selective for citrobacter freundii-derived alleles of plasmid ampc genes, we generated a bayesian consensus phylogeny of the published ampc sequences and compared the mics of 16 beta-lactam antibiotics for escherichia coli strains containing cloned copies of the c. freundii ampc alleles. we found that for the majority of compounds investigated, there has been essentially no increase in beta-lactam resistance conferred by t ... | 2002 | 11959544 |
| response of endophytic bacterial communities in potato plants to infection with erwinia carotovora subsp. atroseptica. | the term endophyte refers to interior colonization of plants by microorganisms that do not have pathogenic effects on their hosts, and various endophytes have been found to play important roles in plant vitality. in this study, cultivation-independent terminal restriction fragment length polymorphism analysis of 16s ribosomal dna directly amplified from plant tissue dna was used in combination with molecular characterization of isolates to examine the influence of plant stress, achieved by infec ... | 2002 | 11976096 |
| monoassociation of scid mice with helicobacter muridarum, but not four other enterics, provokes ibd upon receipt of t cells. | recently, a number of animal models for different aspects of inflammatory bowel disease (ibd) have been developed. the aim of this study was to use one of these to determine whether particular, ostensibly innocuous, intestinal bacteria could provoke or exacerbate ibd. | 2002 | 11984521 |
| d,l-hydantoinase activity of an ochrobactrum anthropi strain. | a microorganism with the ability to release methionine from d,l-(2-methylthioethyl) hydantoin (strain 245) was isolated from soil. the aim of this study was the identification of the strain and the adjustment of the conditions of growth and of the enzymatic reaction, in order to achieve high specific activities of bioconversion of the hydantoin. | 2002 | 12010542 |
| evaluation of the dipstreak, a new device with an original streaking mechanism for detection, counting, and presumptive identification of urinary tract pathogens. | dipstreak is a new urine culture device with two types of agar attached back-to-back on a plastic paddle. it combines dip-slide technology and an original streaking inoculation mechanism, allowing for bacterial counting and colony isolation. the performance of the dipstreak device with two different medium formulations, chromagar and macconkey media in study a and uriselect 3 and macconkey media in study b, was evaluated and compared to that of the reference streak method by using plates of cyst ... | 2002 | 12037082 |
| prevalent bacterial species and novel phylotypes in advanced noma lesions. | the purpose of this study was to determine the bacterial diversity in advanced noma lesions using culture-independent molecular methods. 16s ribosomal dna bacterial genes from dna isolated from advanced noma lesions of four nigerian children were pcr amplified with universally conserved primers and spirochetal selective primers and cloned into escherichia coli. partial 16s rrna sequences of approximately 500 bases from 212 cloned inserts were used initially to determine species identity or close ... | 2002 | 12037085 |
| population analysis of a binary bacterial culture by multi-parametric flow cytometry. | to study the degradation of a xenobiotic that requires a mixed culture it is essential to monitor the proportions and to control the population dynamics of the component strains. for these purposes fluorochromising techniques and multi-parametric flow cytometry were used to follow rhodococcus erythropolis k2-3 and ochrobactrum anthropi k2-14, both of which are needed to degrade 4-(2,4-dichlorophenoxy)butyric acid (2,4-db). although the two strains can grow in constant proportions in mixed cultur ... | 2002 | 12067522 |
| cloning and heterologous expression of an enantioselective amidase from rhodococcus erythropolis strain mp50. | the gene for an enantioselective amidase was cloned from rhodococcus erythropolis mp50, which utilizes various aromatic nitriles via a nitrile hydratase/amidase system as nitrogen sources. the gene encoded a protein of 525 amino acids which corresponded to a protein with a molecular mass of 55.5 kda. the deduced complete amino acid sequence showed homology to other enantioselective amidases from different bacterial genera. the nucleotide sequence approximately 2.5 kb upstream and downstream of t ... | 2002 | 12089004 |
| biodiversity of denitrifying and dinitrogen-fixing bacteria in an acid forest soil. | isolated soil dna from an oak-hornbeam forest close to cologne, germany, was suitable for pcr amplification of gene segments coding for the 16s rrna and nitrogenase reductase (nifh), nitrous oxide reductase (nosz), cytochrome cd(1)-containing nitrite reductase (nirs), and cu-containing nitrite reductase (nirk) of denitrification. for each gene segment, diverse pcr products were characterized by cloning and sequencing. none of the 16s rrna gene sequences was identical to any deposited in the data ... | 2002 | 12147477 |
| vertebral osteomylitis due to ochrobactrum anthropi. | | 2002 | 12162404 |
| the effects of nacl and some heavy metals on the denitrification activity of ochrobactrum anthropi. | ochrobactrum anthropi is a well-known gram-negative bacterium, with the ability to degrade atrazine, urea-formaldehyde and chlorophenols. investigation were made of the nitrate and nitrite reduction capacities of the strain in succinate and glucose media, and the tolerance of its denitrification to nacl and some heavy metals. succinate proved to be a better carbon source to drive denitrification by o. anthropi. batch fermentation studies in anaerobic succinate medium indicated reduction capaciti ... | 2002 | 12210551 |
| inducible ampc beta-lactamase of a new member enterobacteriaceae. | extensive biochemical testing and 16s rrna and rpob sequence analysis revealed that clinical strain cf01ent1, initially identified as buttiauxella agrestis by the use of api 32 biochemical strips, is a new organism in the enterobacteriaceae family. it produced an inducible ampc-type beta-lactamase whose sequence shares 69 to 72% identity with those of the other ampc-type beta-lactamases of enterobacteriaceae: this enzyme exhibits an atypical high affinity for all beta-lactams tested. | 2002 | 12234870 |
| comparative genome analysis of the alpha -proteobacteria: relationships between plant and animal pathogens and host specificity. | | 2002 | 12271145 |
| ochrobactrum anthropi bloodstream infection complicating hemodialysis. | we report two cases of bacteremia with ochrobactrum anthropi in patients on hemodialysis. bacteremia became clinically manifest by recurrent febrile episodes during and after dialysis. in one patient, bacteremia persisted after ciprofloxacin therapy and was cleared only by removal of the dialysis catheter and a 3-week course of gentamicin. the second patient remained intermittently bacteremic for more than 3 months, although the dialysis catheter had been replaced. a medline search revealed only ... | 2002 | 12324938 |
| microbial population in a hydrogen-dependent denitrification reactor. | the bacterial population in an h2-dependent denitrification system was studied. the laboratory set-up was designed for the treatment of potable water and consisted of an electrochemical cell, where the water to be treated was enriched with h2 prior to entering a bioreactor. bioreactors (columns packed with granulated active carbon) were inoculated with denitrifying bacterial strains isolated from a previous reactor, then sampled immediately after inoculation, or after 1 or 3 months of continuous ... | 2002 | 12405417 |
| rhodanobacter sp. strain bpc1 in a benzo[a]pyrene-mineralizing bacterial consortium. | a bacterial consortium which rapidly mineralizes benzo[a]pyrene when it is grown on a high-boiling-point diesel fuel distillate (hbd) was recovered from soil and maintained for approximately 3 years. previous studies have shown that mobilization of benzo[a]pyrene into the supernatant liquid precedes mineralization of this compound (r. kanaly, r. bartha, k. watanabe, and s. harayama, appl. environ. microbiol. 66:4205-4211, 2000). in the present study, we found that sterilized supernatant liquid f ... | 2002 | 12450801 |
| molecular characterization of a salt-tolerant bacterial community in the rice rhizosphere. | the diversity of salt-tolerant bacteria present in the rhizosphere of oryza sativa was investigated. fourteen bacterial strains, isolated after enrichment in nitrogen-free, semi-solid medium and showing tolerance to 3% nacl, were analyzed by restriction patterns produced by amplified dna coding for 16s rdna (ardra) with enzymes sau3ai, alui and rsai which showed that they were represented by 4 ardra types. biodiversity among the 14 strains was also analyzed by the random amplified polymorphic dn ... | 2002 | 12455705 |
| biochemistry and comparative genomics of sxxk superfamily acyltransferases offer a clue to the mycobacterial paradox: presence of penicillin-susceptible target proteins versus lack of efficiency of penicillin as therapeutic agent. | the bacterial acyltransferases of the sxxk superfamily vary enormously in sequence and function, with conservation of particular amino acid groups and all-alpha and alpha/beta folds. they occur as independent entities (free-standing polypeptides) and as modules linked to other polypeptides (protein fusions). they can be classified into three groups. the group i sxxk d,d-acyltransferases are ubiquitous in the bacterial world. they invariably bear the motifs sxxk, sxn(d), and kt(s)g. anchored in t ... | 2002 | 12456788 |
| development and characterization of murine monoclonal antibodies specific for dissimilatoric copper nitrite reductase. | several hybridoma cell lines from mice were established, producing monoclonal antibodies (mabs) directed against the dissimilatoric copper nitrite reductase (dnir) to detect actual denitrifying bacteria at the single cell level under nondestructive conditions in the environment. the mice were immunized with native or recombinant enzyme gained from two different bacteria, ochrobactrum anthropi and alcaligenes faecalis. the antibodies obtained could be divided into two groups according to their di ... | 2002 | 12470477 |
| genetic diversity and spoilage potentials among pseudomonas spp. isolated from fluid milk products and dairy processing plants. | degradation of milk components through various enzymatic activities associated with the contamination of dairy products by pseudomonas spp. can reduce the shelf life of processed milk. reliable methods for differentiating among pseudomonas spp. strains are necessary to identify and eliminate specific sources of bacterial contamination from dairy processing systems. to that end, we assessed the genetic diversity and dairy product spoilage potentials among a total of 338 pseudomonas spp. isolates ... | 2003 | 12513987 |
| characteristics of massilia timonae and massilia timonae-like isolates from human patients, with an emended description of the species. | the description of massilia timonae, a nonfermentative aerobic gram-negative rod, was based on a single strain. a subsequent report of a second isolate has been recently published. phenotypic descriptions of these two strains were based primarily on commercial test kit results. we have identified three additional strains as m. timonae by 16s rrna sequence analysis and have characterized them phenotypically in parallel with the type strain of m. timonae, cip 105350, by conventional test methods. ... | 2003 | 12517847 |
| evaluation of microscan autoscan for identification of pseudomonas aeruginosa isolates from cystic fibrosis patients. | accurate identification of gram-negative bacilli from cystic fibrosis (cf) patients is essential. only 57% (108 of 189) of nonmucoid strains and 40% (24 of 60) of mucoid strains were definitively identified as pseudomonas aeruginosa with microscan autoscan. most common misidentifications were pseudomonas fluorescens-pseudomonas putida (i.e., the strain was either p. fluorescens or p. putida, but the system did not make the distinction and yielded the result p. fluorescens/putida) and alcaligenes ... | 2003 | 12517904 |
| characterization of a thermostable d-stereospecific alanine amidase from brevibacillus borstelensis bcs-1. | a gene encoding a new thermostable d-stereospecific alanine amidase from the thermophile brevibacillus borstelensis bcs-1 was cloned and sequenced. the molecular mass of the purified enzyme was estimated to be 199 kda after gel filtration chromatography and about 30 kda on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, indicating that the enzyme could be composed of a hexamer with identical subunits. the purified enzyme exhibited strong amidase activity towards d-amino acid-containin ... | 2003 | 12571020 |
| ochrobactrum anthropi bacteraemia in immunocompetent children. | ochrobactrum anthropi is an emerging pathogen in immunocompromised patients but infections with the bacterium have very rarely been documented in normal hosts. we report the characteristics of o. anthropi bacteraemia in 11 immunocompetent children, aged 2 months to 7 y, hospitalized in a general hospital during a 5-y period. children commonly presented with fever, non-specific respiratory or gastrointestinal manifestations, leukocytosis and neutrophilia and had a rapid recovery, even when they d ... | 2002 | 12578145 |
| transcriptome analysis of sinorhizobium meliloti during symbiosis. | rhizobia induce the formation on specific legumes of new organs, the root nodules, as a result of an elaborated developmental program involving the two partners. in order to contribute to a more global view of the genetics underlying this plant-microbe symbiosis, we have mined the recently determined sinorhizobium meliloti genome sequence for genes potentially relevant to symbiosis. we describe here the construction and use of dedicated nylon macroarrays to study simultaneously the expression of ... | 2003 | 12620125 |
| septic shock caused by ochrobactrum anthropi in an otherwise healthy host. | reported is a case of life-threatening septic shock that occurred in an otherwise healthy host after administration of a peripheral venous infusion of a solution contaminated with ochrobactrum anthropi, an unusual human pathogen. the rapid onset of shock may have been due to a large inoculum caused by nonsterile practices at the time of reconstitution. | 2003 | 12624082 |
| [species identification and molecular epidemiology of bacteria belonging to ochrobactrum genus]. | two species of medical interest belong to the genus ochrobactrum, ochrobactrum anthropi and ochrobactrum intermedium. they are members of the microbiota of soil and an increasing number of works report the isolation of o. anthropi from clinical specimen, especially from immunocompromised patients and nosocomial infection. involving of each species in human infection is poorly estimated due to unclear differential phenotypic characters. we performed 16s rdna sequencing for identification of 20 cl ... | 2003 | 12628286 |
| liquid chromatography-electrospray mass spectrometry study of cysteine-10 s-glutathiolation in recombinant glutathione s-transferase of ochrobactrum anthropi. | glutathione s-transferase of ochrobactrum anthropi (oagst), a bacterium isolated from soils contaminated by xenobiotic pollutants, was recently purified, cloned and characterised in our laboratories. the recombinant oagst (roagst), highly expressed in escherichia coli, when purified by glutathione-affinity chromatography and then analysed by electrospray ionisation mass spectrometry (esi-ms), has evidenced a disulphide bond with glutathione (s-glutathiolation), which was removable by reduction w ... | 2003 | 12650762 |
| atypical 16s rrna gene copies in ochrobactrum intermedium strains reveal a large genomic rearrangement by recombination between rrn copies. | ochrobactrum intermedium is an opportunistic human pathogen belonging to the alpha 2 subgroup of proteobacteria. the 16s rdna sequences of nine o. intermedium isolates from a collection of clinical and environmental isolates exhibited a 46-bp insertion at position 187, which was present in only one sequence among the 82 complete or partial 16s rdna sequences of ochrobactrum spp. available in data banks. reverse transcription-pcr experiments showed that the 46-bp insertion remained in the 16s rrn ... | 2003 | 12700269 |
| misidentification of brucella melitensis as ochrobactrum anthropi by api 20ne. | | 2003 | 12721322 |
| discordant phylogenies within the rrn loci of rhizobia. | it is evident from complete genome sequencing results that lateral gene transfer and recombination are essential components in the evolutionary process of bacterial genomes. since this has important implications for bacterial systematics, the primary objective of this study was to compare estimated evolutionary relationships among a representative set of alpha-proteobacteria by sequencing analysis of three loci within their rrn operons. tree topologies generated with 16s rrna gene sequences were ... | 2003 | 12730157 |
| evaluation of the vitek 2 id-gnb assay for identification of members of the family enterobacteriaceae and other nonenteric gram-negative bacilli and comparison with the vitek gni+ card. | we evaluated the vitek 2 id-gnb identification card (biomérieux, inc., durham, n.c.) for its ability to identify members of the family enterobacteriaceae and other gram-negative bacilli that are isolated in clinical microbiology laboratories. using 482 enteric stock cultures and 103 strains of oxidase-positive, gram-negative glucose-fermenting and nonfermenting bacilli that were maintained at -70 degrees c and passaged three times before use, we inoculated cards according to the manufacturer's d ... | 2003 | 12734254 |
| [nosocomial sepsis due to ochrobactrum anthropi in hiv positive patients: two case reports] | the first two case reports of nosocomial ochrobactrum anthropi septicemia occurring in patients with hiv disease are presented, and discussed in light of recent evidence of non-fermenting gran-negative bacilli as emerging pathogens in hospitalized immunocompromised patients. among patients with advanced hiv infection, o. anthropi septicemia may occur even when certain presumed risk factors (notably indwelling catheters and instrumentation) are lacking, while a low cd4+ lymphocyte count, neutrope ... | 1999 | 12759592 |
| towards a standardized format for the description of a novel species (of an established genus): ochrobactrum gallinifaecis sp. nov. | a format for the description of single novel species is proposed, which should facilitate the reviewing process by assisting the provision of data in a standardized form. the abstract must be short and concise, highlighting phylogenetic position, morphology and chemotaxonomy for genus affiliation, the genotypic and phenotypic basis for species differentiation, and the name and deposition numbers from two public culture collections in different countries for the type strain: a gram-negative, rod- ... | 2003 | 12807218 |
| heavy metal biosorption by biomass of ochrobactrum anthropi producing exopolysaccharide in activated sludge. | the removal of chromium, cadmium and copper, toxic metals of high environmental priority due to their toxicity, from dilute aqueous solutions has been studied in the present work, applying a dead exopolysaccharide producing bacterium, ochrobactrum anthropi, isolated from activated sludge. particularly, the effect of ph, metal concentration and the effects of contact time were considered. optimum adsorption ph values of chromium(vi), cadmium(ii) and copper(ii) were 2.0, 8.0 and 3.0 respectively. ... | 2003 | 12835060 |
| real-time pcr detection of brucella abortus: a comparative study of sybr green i, 5'-exonuclease, and hybridization probe assays. | real-time pcr provides a means of detecting and quantifying dna targets by monitoring pcr product accumulation during cycling as indicated by increased fluorescence. a number of different approaches can be used to generate the fluorescence signal. three approaches-sybr green i (a double-stranded dna intercalating dye), 5'-exonuclease (enzymatically released fluors), and hybridization probes (fluorescence resonance energy transfer)-were evaluated for use in a real-time pcr assay to detect brucell ... | 2003 | 12902268 |
| ochrobactrum anthropi misidentified as shewanella putrefaciens. | | 2003 | 12958302 |
| detection methods for the expression of the dissimilatory copper-containing nitrite reductase gene (dnirk) in environmental samples. | in situ assays, based on monoclonal antibodies (mabs), were developed to study the microbial expression of the bacterial dissimilatory copper-containing nitrite reductase gene (dnirk), one of the key enzymes involved in denitrification, in different ecosystems. with a combination of an anti-dnirk mab and phylogenetic oligonucleotide probes, it is possible to bring structural and functional aspects of microbial communities together. to perform a double labelling, yielding a high signal strength f ... | 2003 | 14499994 |
| analysis of bacterial dna patterns--an approach for controlling biotechnological processes. | optimisation of biotechnological processes catalysed by microbial cells requires detailed information about operational limits of the single cells. their performance is correlated with distinct physiological states. we related these states to cell cycle events, which were found to proceed extremely diversely in different bacterial strains. characteristic dna patterns were found flow cytometrically, depending on the type of strain, substrates and growth conditions involved; this information can b ... | 2003 | 14607431 |
| genes for an alkaline d-stereospecific endopeptidase and its homolog are located in tandem on bacillus cereus genome. | alkaline d-peptidase (adp) from bacillus cereus df4-b is a d-stereospecific endopeptidase acting on oligopeptides composed of d-phenylalanine and the primary structure deduced from its gene, adp, shows a similarity with d-stereospecific hydrolases from ochrobactrum anthropi strains. we have isolated dna fragments covering the flanking region of adp from df4-b genome and found an additional gene, adp2, located upstream of adp. the deduced amino acid sequence of adp2 showed 96% and 85% identity wi ... | 2003 | 14612229 |
| protein identification from two-dimensional gel electrophoresis analysis of klebsiella pneumoniae by combined use of mass spectrometry data and raw genome sequences. | separation of proteins by two-dimensional gel electrophoresis (2-de) coupled with identification of proteins through peptide mass fingerprinting (pmf) by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) is the widely used technique for proteomic analysis. this approach relies, however, on the presence of the proteins studied in public-accessible protein databases or the availability of annotated genome sequences of an organism. in this work, we investig ... | 2003 | 14653859 |
| use of borate to control the 5'-position-selective microbial glucosylation of pyridoxine. | nearly 100% 5'-position selectivity of transglucosylation from maltodextrin to pyridoxine (pn) by cells of verticillium dahliae tpu 4900 was observed when the reaction was carried out with borate. the same effect of borate was observed not only during synthesis of pyridoxine 5'-alpha-d-glucoside by partially purified enzyme of this strain but also during synthesis of this compound by other microorganisms and with other enzymes (alpha-glucosidase and cyclomaltodextrin glucanotransferase). the eff ... | 2003 | 14660349 |
| ochrobactrum anthropi bacteraemia. | | 2003 | 14723384 |
| real-time multiplex pcr assay for detection of brucella spp., b. abortus, and b. melitensis. | the identification of brucella can be a time-consuming and labor-intensive process that places personnel at risk for laboratory-acquired infection. here, we describe a real-time pcr assay for confirmation of presumptive brucella isolates. the assay was designed in a multiplex format that will allow the rapid identification of brucella spp., b. abortus, and b. melitensis in a single test. | 2004 | 15004098 |
| validated 5' nuclease pcr assay for rapid identification of the genus brucella. | a real-time, genus-specific 5' nuclease pcr assay for amplification of a 322-bp fragment of the per gene was developed for rapid (<2 h) identification of brucella spp. from agar plates. the assay, including an internal amplification control (116 bp), identified brucella strains (n = 23) and did not detect non-brucella strains (n = 174), indicating its usefulness, particularly for laboratories with stringent quality assurance programs. | 2004 | 15131207 |
| ammonium and methylammonium uptake in a fertilizer-degrading strain of ochrobactrum anthropi. | the transport of ammonium and methylammonium was studied in a strain of ochrobactrum anthropi, a microorganism isolated from garden soil and able to degrade methyleneureas which are used as slow-release nitrogen fertilizer. the activity of both transport systems was determined using [14c]methylammonium. differences between the two transport systems were observed with regard to their ph- and temperature dependence as well as their kinetic parameters and regulation during growth with various nitro ... | 2000 | 15188892 |
| glutathione-dependent biotransformation of the fungicide chlorothalonil. | a gene responsible for the chlorothalonil biotransformation was cloned from the chromosomal dna of ochrobactrum anthropi sh35b, capable of efficiently dissipating the chlorothalonil. the gene encoding glutathione s-transferase (gst) of o. anthropi sh35b was expressed in escherichia coli, and the gst was subsequently purified by affinity chromatography. the fungicide chlorothalonil was rapidly transformed by the gst in the presence of glutathione. lc-ms analysis supported the formation of mono-, ... | 2004 | 15212468 |
| prosthetic mitral valve endocarditis due to ochrobactrum anthropi: case report. | we describe a case of infective endocarditis in a prosthetic mitral valve due to ochrobactrum anthropi. although o. anthropi is an emerging pathogen in immunocompromised patients, infections with the bacterium have very rarely been documented in healthy hosts, and endocarditis is rare. to our knowledge, only two cases of o. anthropi endocarditis have been reported in the medical literature. | 2004 | 15243121 |
| ecological significance of microdiversity: identical 16s rrna gene sequences can be found in bacteria with highly divergent genomes and ecophysiologies. | a combination of cultivation-based methods with a molecular biological approach was used to investigate whether planktonic bacteria with identical 16s rrna gene sequences can represent distinct eco- and genotypes. a set of 11 strains of brevundimonas alba were isolated from a bacterial freshwater community by conventional plating or by using a liquid most-probable-number (mpn) dilution series. these strains had identical 16s rrna gene sequences and represented the dominant phylotype in the plate ... | 2004 | 15294821 |
| use of 16s rrna gene sequencing for rapid confirmatory identification of brucella isolates. | members of the genus brucella are categorized as biothreat agents and pose a hazard for both humans and animals. current identification methods rely on biochemical tests that may require up to 7 days for results. we sequenced the 16s rrna genes of 65 brucella strains along with 17 related strains likely to present a differential diagnostic challenge. all brucella 16s rrna gene sequences were determined to be identical and were clearly different from the 17 related strains, suggesting that 16s rr ... | 2004 | 15297511 |
| interaction of endotoxins with toll-like receptor 4 correlates with their endotoxic potential and may explain the proinflammatory effect of brucella spp. lps. | endotoxins displaying differences in the chemical structure of their lipid a were used to induce the expression of chemokines in the human monocytic thp-1 cell line. lps from two enterobacterial species such as escherichia coli and yersinia enterocolitica induced mrna expression of ifn-gamma-inducible protein (ip)-10, macrophage-inflammatory protein (mip)-1alpha, mip-1beta, monocyte chemoattractant protein (mcp)-1 and il-8. lps from the non-enterobacterial genera brucella and ochrobactrum induce ... | 2004 | 15339879 |
| x-ray crystal structure of ornithine acetyltransferase from the clavulanic acid biosynthesis gene cluster. | the orf6 gene from the clavulanic acid biosynthesis gene cluster encodes an oat (ornithine acetyltransferase). similar to other oats the enzyme has been shown to catalyse the reversible transfer of an acetyl group from n-acetylornithine to glutamate. oats are ntn (n-terminal nucleophile) enzymes, but are distinct from the better-characterized ntn hydrolase enzymes as they catalyse acetyl transfer rather than a hydrolysis reaction. in the present study, we describe the x-ray crystal structure of ... | 2005 | 15352873 |
| occurrence and potential diagnostic applications of serological cross-reactivities between brucella and other alpha-proteobacteria. | agrobacterium, sinorhizobium, and ochrobactrum are genera closely related to brucella but, in contrast to the latter, are not pathogenic for humans and animals. we studied by an indirect enzyme-linked immunosorbent assay (elisa) the reactivities of brucellosis sera against cytosolic (cyt) and membrane (ma) antigens from these nonpathogenic bacteria, and we evaluated the potential usefulness of these cross-reactions for the diagnosis of brucellosis in humans, sheep, cows, and dogs. canine infecti ... | 2004 | 15358645 |
| involvement of linear plasmids in aerobic biodegradation of vinyl chloride. | pseudomonas putida strain aj and ochrobactrum strain td were isolated from hazardous waste sites based on their ability to use vinyl chloride (vc) as the sole source of carbon and energy under aerobic conditions. strains aj and td also use ethene and ethylene oxide as growth substrates. strain aj contained a linear megaplasmid (approximately 260 kb) when grown on vc or ethene, but it contained no circular plasmids. while strain aj was growing on ethylene oxide, it was observed to contain a 100-k ... | 2004 | 15466555 |
| characterization of bacterial community diversity in cystic fibrosis lung infections by use of 16s ribosomal dna terminal restriction fragment length polymorphism profiling. | progressive loss of lung function resulting from the inflammatory response to bacterial colonization is the leading cause of mortality in cystic fibrosis (cf) patients. a greater understanding of these bacterial infections is needed to improve lung disease management. as culture-based diagnoses are associated with fundamental drawbacks, we used terminal restriction fragment (t-rf) length polymorphism profiling and 16s rrna clone data to characterize, without prior cultivation, the bacterial comm ... | 2004 | 15528712 |
| expression of glutathione s-transferase and peptide methionine sulphoxide reductase in ochrobactrum anthropi is correlated to the production of reactive oxygen species caused by aromatic substrates. | peptide methionine sulphoxide reductase (msra) and glutathione s-transferases (gsts) are considered as detoxification enzymes. in the xenobiotics-degrading bacterium ochrobactrum anthropi the two enzymes are co-induced by toxic concentrations of aromatic substrates such as phenol and 4-chlorophenol. in aerobic organisms, degradation of aromatic substrates by mono- and dioxygenases leads to a generation of oxidative stress that causes the occurrence of reactive oxygen species (ros). a capillary e ... | 2004 | 15598526 |
| paraquat resistance of transgenic tobacco plants over-expressing the ochrobactrum anthropi pqra gene. | transgenic tobacco plants over-expressing the ochrobactrum anthropi pqra gene, which encodes a membrane transporter mediating resistance to paraquat, were generated. transgenic plants displayed higher resistance against paraquat than wild-type plants, as estimated by plant viability, ion leakage and chlorophyll loss, but no resistance against other active oxygen generators, such as h2o2 and menadione. moreover, lower levels of paraquat accumulated in transgenic plants, compared to wild-type plan ... | 2004 | 15604769 |
| diversity of green-like and red-like ribulose-1,5-bisphosphate carboxylase/oxygenase large-subunit genes (cbbl) in differently managed agricultural soils. | a pcr-based approach was developed to detect ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) form i large-subunit genes (cbbl) as a functional marker of autotrophic bacteria that fix carbon dioxide via the calvin-benson-bassham cycle. we constructed two different primer sets, targeting the green-like and red-like phylogenetic groups of cbbl genes. the diversity of these cbbl genes was analyzed by the use of three differently managed agricultural soils from a long-term field experiment. ... | 2005 | 15640185 |
| manual and automated instrumentation for identification of enterobacteriaceae and other aerobic gram-negative bacilli. | identification of gram-negative bacilli, both enteric and nonenteric, by conventional methods is not realistic for clinical microbiology laboratories performing routine cultures in today's world. the use of commercial kits, either manual or automated, to identify these organisms is a common practice. the advent of rapid or "spot" testing has eliminated the need for some commonly isolated organisms to be identified with the systems approach. commercially available systems provide more in-depth id ... | 2005 | 15653824 |
| molecular identification of a novel deltaproteobacterium as the etiologic agent of epizootic bovine abortion (foothill abortion). | epizootic bovine abortion (eba) is endemic in california's coastal range and the foothill regions of the sierra nevada, where it has been the primary diagnosed cause of abortion in beef cattle for >50 years. investigation of these losses has defined a specific fetal syndrome characterized by late-term abortion or birth of weak or dead calves. although the unusual clinical presentation and unique fetal pathology associated with eba have been recognized since the 1950s, the identity of the etiolog ... | 2005 | 15695652 |
| [ochrobactrum anthropi infectious endocarditis in an immunocompetent patient]. | | 2005 | 15743587 |
| nodulation of lupinus albus by strains of ochrobactrum lupini sp. nov. | the nodulation of legumes has for more than a century been considered an exclusive capacity of a group of microorganisms commonly known as rhizobia and belonging to the alpha-proteobacteria. however, in the last 3 years four nonrhizobial species, belonging to alpha and beta subclasses of the proteobacteria, have been described as legume-nodulating bacteria. in the present study, two fast-growing strains, lup21 and lup23, were isolated from nodules of lupinus honoratus. the phylogenetic analysis ... | 2005 | 15746334 |
| genotyping of ochrobactrum spp. by aflp analysis. | aflp was used to analyze the genetic diversity among ochrobactrum strains. aflp patterns showed a great genomic variability that separated the samples into three distinct clusters. ochrobactrum intermedium was found to be closely related to brucella abortus s99. | 2005 | 15774899 |
| cloning and characterization of a chromosomal class c beta-lactamase and its regulatory gene in laribacter hongkongensis. | laribacter hongkongensis, a newly discovered bacterium recently shown to be associated with community-acquired gastroenteritis, is generally resistant to most beta-lactams except the carbapenems. we describe the cloning and characterization of a novel chromosomal class c beta-lactamase and its regulatory gene in l. hongkongensis. two genes, ampc and ampr, were cloned by inserting restriction fragments of genomic dna from l. hongkongensis strain hlhk5 into pbk-cmv to give the recombinant plasmid ... | 2005 | 15855519 |
| dynamic kinetic resolution of amino acid amide catalyzed by d-aminopeptidase and alpha-amino-epsilon-caprolactam racemase. | amino acid amide racemizing activity was discovered in alpha-amino-epsilon-caprolactam (acl) racemase (ec 5. 1. 1. 15) from achromobacter obae. the enzymatic synthesis of d-alanine from l-alanine amide has been demonstrated by use of d-aminopeptidase (dap; ec 3. 4. 11. 19) from ochrobactrum anthropi c1-38 and acl racemase. the conversion of 45 mm l-alanine amide was carried out at 30 degrees c for 7 h; l-alanine amide was completely converted to d-alanine, and no l-alanine was detected. the resu ... | 2005 | 15913357 |
| dom-fold: a structure with crossing loops found in dmpa, ornithine acetyltransferase, and molybdenum cofactor-binding domain. | understanding relationships between sequence, structure, and evolution is important for functional characterization of proteins. here, we define a novel dom-fold as a consensus structure of the domains in dmpa (l-aminopeptidase d-ala-esterase/amidase), oat (ornithine acetyltransferase), and mocobd (molybdenum cofactor-binding domain), and discuss possible evolutionary scenarios of its origin. as shown by a comprehensive structure similarity search, dom-fold distinguished by a two-layered beta/al ... | 2005 | 15937278 |
| a dmpa-homologous protein from pseudomonas sp. is a dipeptidase specific for beta-alanyl dipeptides. | we have determined the nucleotide sequence of a dna fragment covering the flanking region of the r-stereoselective amidase gene, rama, from the pseudomonas sp. mci3434 genome and found an additional gene, bapa, coding for a protein showing sequence similarity to dmpa aminopeptidase from ochrobactrum anthropi lmg7991 (43% identity). the dmpa (called l-aminopeptidase d-ala-esterase/amidase) hydrolyzes alanine-p-nitroanilide, alaninamide, and alanine methylester with a preference for the d-configur ... | 2005 | 15955066 |
| [infective endocarditis due to ochrobactrum anthropi]. | | 2005 | 15970175 |
| ['in vitro' activity of different antimicrobial agents on gram-negative nonfermentative bacilli, excluding pseudomonas aeruginosa and acinetobacter spp]. | gram-negative nonfermentative bacilli (nfb) are widely spread in the environment. besides of difficulties for identification, they often have a marked multiresistance to antimicrobial agents, including those active against pseudomonas aeruginosa. the objective of this study was to evaluate the 'in vitro' activity of different antimicrobial agents on 177 gram-negative nonfermentative bacilli isolates (excluding pseudomonas aeruginosa and acinetobacter spp.) isolated from clinical specimens. minim ... | 2005 | 15991478 |
| pulsed-field gel electrophoresis to study the diversity of whole-genome organization in the genus ochrobactrum. | the alpha-proteobacterial genus ochrobactrum groups together organisms that display varied life-styles, such as free-living bacteria, members of rhizosphere and soil, nitrogen-fixing bacteria in plant nodules, xenobiotic-degrading bacteria, colonizers of nematodes and insects, and opportunistic human pathogens. the genomes of nine strains of ochrobactrum anthropi and eight strains of ochrobactrum intermedium were analyzed by pulsed-field gel electrophoresis of the whole genome and of i-ceui dige ... | 2005 | 15995979 |
| superiority of molecular techniques for identification of gram-negative, oxidase-positive rods, including morphologically nontypical pseudomonas aeruginosa, from patients with cystic fibrosis. | phenotypic identification of gram-negative bacteria from cystic fibrosis (cf) patients carries a high risk of misidentification. therefore, we compared the results of biochemical identification by api 20ne with 16s rrna gene sequencing in 88 gram-negative, oxidase-positive rods, other than morphologically and biochemically typical p. aeruginosa, from respiratory secretions of cf patients. the api 20ne allowed correct identification of the bacterial species in 15 out of 88 (17%) isolates investig ... | 2005 | 16081953 |
| specificity inversion of ochrobactrum anthropi d-aminopeptidase to a d,d-carboxypeptidase with new penicillin binding activity by directed mutagenesis. | the serine penicillin-recognizing proteins have been extensively studied. they show a wide range of substrate specificities accompanied by multidomain features. their adaptation capacity has resulted in the emergence of pathogenic bacteria resistant to beta-lactam antibiotics. the most divergent enzymatic activities in this protein family are those of the ochrobactrum anthropi d-aminopeptidase and of the streptomyces r61 d,d-carboxypeptidase/transpeptidase. with the help of structural data, we h ... | 2005 | 16131658 |
| molecular and phenotypic features for identification of the opportunistic pathogens ochrobactrum spp. | among the six species characterized within the genus ochrobactrum, ochrobactrum anthropi and ochrobactrum intermedium are currently reported as opportunistic pathogens in humans. since the species identification is mainly based on 16s rdna analysis, the aim of this study was to search for other characteristics useful for ochrobactrum species discrimination. ribotyping, morphological and biochemical analyses, and antimicrobial susceptibility testing were performed for a panel of 35 clinical isola ... | 2005 | 16157548 |
| identification and analysis of the promoter region of the human methionine sulphoxide reductase a gene. | msra (methionine sulphoxide reductase a) is an antioxidant repair enzyme that reduces oxidized methionine to methionine. moreover, the oxidation of methionine residues in proteins is considered to be an important consequence of oxidative damage to cells. to understand mechanisms of human msra gene expression and regulation, we cloned and characterized the 5' promoter region of the human msra gene. using 5'-race (rapid amplification of cdna ends) analysis of purified mrna from human cells, we loc ... | 2006 | 16162094 |
| identification and analysis of the promoter region of the human methionine sulphoxide reductase a gene. | msra (methionine sulphoxide reductase a) is an antioxidant repair enzyme that reduces oxidized methionine to methionine. moreover, the oxidation of methionine residues in proteins is considered to be an important consequence of oxidative damage to cells. to understand mechanisms of human msra gene expression and regulation, we cloned and characterized the 5' promoter region of the human msra gene. using 5'-race (rapid amplification of cdna ends) analysis of purified mrna from human cells, we loc ... | 2006 | 16162094 |
| evaluation of ochrobactrum intermedium bacteremia in a patient with bladder cancer. | it is generally believed that ochrobactrum anthropi is the only pathogenic species in the genus ochrobactrum. we report a case of presumptive ochrobactrum intermedium bacteremia in a patient with bladder cancer. identification of a rare species using rapid commercial panel could lead to an erroneous result as this clinical isolate was initially reported to be shewanella putrefaciens. the patient was successfully treated with imipenem and ciprofloxacin. | 2005 | 16168615 |
| [duplicate publication: infectious endocarditis by ochrobactrum anthropi on immunocompetent patient]. | | 2005 | 16185575 |
| complete mineralisation of dimethylformamide by ochrobactrum sp. dgvk1 isolated from the soil samples collected from the coalmine leftovers. | a bacterial strain dgvk1 capable of using n,n-dimethylformamide (dmf) as sole source of carbon and nitrogen was isolated from the soil samples collected from the coalmine leftovers. the molecular phylogram generated using the complete sequence of 16s rdna of the strain dgvk1 showed close links to the bacteria grouped under brucellaceae family that belongs to alphaproteobacteria class. specifically, the 16s rdna sequence of strain dgvk1 has shown 97% similarity to ochrobactrum anthropi lmg 3331 ( ... | 2006 | 16211382 |
| bacterial communities in petroleum oil in stockpiles. | bacterial communities in crude-oil samples from japanese oil stockpiles were investigated by 16s rrna gene cloning, followed by denaturing gradient gel electrophoresis (dgge) analysis. 16s rrna genes were successfully amplified by pcr after isooctane treatment from three kinds of crude-oil sample collected at four oil stockpiles in japan. dgge profiles showed that bacteria related to ochrobactrum anthropi, burkholderia cepacia, stenotrophomonas maltophilia, propionibacterium acnes, and brevundim ... | 2005 | 16233771 |
| delta-(d -alpha-aminoadipoyl)-cleaving amidase of ochrobactrum anthropi. | ochrobactrum anthropi cleaved the delta-(d-alpha-aminoadipoyl)-side chain from delta-(d-alpha-aminoadipoyl)-7-amino-4-methylcoumarin, a beta-lactamase-resistant cephalosporin c analogue. in whole cell conversions up to 1 nkat g(-1) dry cell wt were achieved. o. anthropi possesses also gamma-d-glutamyltranspeptidase activity, 8 nkat g(-1) dry cell wt, the likely cause of delta-(d-alpha-aminoadipoyl)-cleavage. | 2005 | 16328990 |
| l-selective amidase with extremely broad substrate specificity from ochrobactrum anthropi ncimb 40321. | an industrially attractive l-specific amidase was purified to homogeneity from ochrobactrum anthropi ncimb 40321 wild-type cells. the purified amidase displayed maximum initial activity between ph 6 and 8.5 and was fully stable for at least 1 h up to 60 degrees c. the purified enzyme was strongly inhibited by the metal-chelating compounds edta and 1,10-phenanthroline. the activity of the edta-treated enzyme could be restored by the addition of zn2+ (to 80%), mn2+ (to 400%), and mg2+ (to 560%). s ... | 2005 | 16332774 |
| light-mediated nitrite accumulation during denitrification by pseudomonas sp. strain jr12. | the effect of light on the denitrifying characteristics of a nonphotosynthetic denitrifier, pseudomonas sp. strain jr12, was examined. already at low light intensities, nitrite accumulated as a result of light inhibition of nitrite but not of nitrate reduction rates. exposure of this bacterium to light caused a photooxidation of cytochrome c, an intermediate electron carrier in its respiratory pathway. photoinhibition of nitrite reduction was reversible, as nitrite reduction rates returned to pr ... | 1998 | 16349525 |
| development and evaluation of a real-time pcr assay targeting the type iii secretion system of burkholderia pseudomallei. | here we report on the development of a discriminatory real-time assay for the rapid identification of burkholderia pseudomallei isolates and the evaluation of this assay for sensitivity against related species and detection in spiked human blood samples. the assay targets a 115-base-pair region within orf2 of the b. pseudomallei type iii secretion system gene cluster and distinguishes b. pseudomallei from other microbial species. assay performance was evaluated with 224 geographically, temporall ... | 2006 | 16390953 |
| genetic and genomic insights into the role of benzoate-catabolic pathway redundancy in burkholderia xenovorans lb400. | transcriptomic and proteomic analyses of burkholderia xenovorans lb400, a potent polychlorinated biphenyl (pcb) degrader, have implicated growth substrate- and phase-dependent expression of three benzoate-catabolizing pathways: a catechol ortho cleavage (ben-cat) pathway and two benzoyl-coenzyme a pathways, encoded by gene clusters on the large chromosome (boxc) and the megaplasmid (boxm). to elucidate the significance of this apparent redundancy, we constructed mutants with deletions of the ben ... | 2006 | 16391095 |
| the effects of salinity and other factors on nitrite reduction by ochrobactrum anthropi 49187. | the nitrite reductase (nir) gene was cloned from ochrobactrum anthropi 49187 and found to contain an open reading frame of 1131 nucleotides, encoding a polypeptide of 376 amino acids. the o. anthropi nir gene encodes a copper-type dissimilatory reductase based on sequence homology with other genes. the polypeptide product is predicted to form a trimeric holoenzyme of 37 kda subunits based on molecular weight estimates of extracts in activity gels. expression of the enzyme is up-regulated by nitr ... | 2006 | 16463313 |
| pelvic abscess due to ochrobactrum intermedium [corrected] in an immunocompetent host: case report and review of the literature. | ochrobactrum intermedium [corrected] infection is rare in humans and is generally associated with immunocompromised hosts with indwelling foreign bodies. we report a case of pelvic abscess with o. intermedium [corrected] after a routine appendectomy in an immunocompetent patient and review the literature on o. intermedium [corrected] infection in patients with normal immune function. | 2006 | 16517927 |
| herbicidal inhibitors of amino acid biosynthesis and herbicide-tolerant crops. | acetohydroxyacid synthase (ahas) inhibitors interfere with branched-chain amino acid biosynthesis by inhibiting ahas. glyphosate affects aromatic amino acid biosynthesis by inhibiting 5-enolpyruvylshikimate-3-phosphate synthase (epsps). glufosinate inhibits glutamine synthetase and blocks biosynthesis of glutamine. ahas gene variants that confer tolerance to ahas inhibitors have been discovered in plants through selection or mutagenesis. imidazolinone-tolerant crops have been commercialized base ... | 2006 | 16547651 |
| genotyping of ochrobactrum anthropi by reca-based comparative sequence, pcr-rflp, and 16s rrna gene analysis. | a reca-pcr restriction fragment length polymorphism assay was developed to study intraspecies variation among ochrobactrum anthropi. primers deduced from the known reca gene sequence of the genetically closely related genus brucella allowed the specific amplification of a 1065 bp reca fragment from each of the 38 o. anthropi and the eight brucella strains investigated. reca was also amplified from the type strains of o. intermedium, o. tritici, and o. lupini but could not be generated from o. gr ... | 2006 | 16553826 |
| 16s rrna gene sequencing versus the api 20 ne system and the vitek 2 id-gnb card for identification of nonfermenting gram-negative bacteria in the clinical laboratory. | over a period of 26 months, we have evaluated in a prospective fashion the use of 16s rrna gene sequencing as a means of identifying clinically relevant isolates of nonfermenting gram-negative bacilli (non-pseudomonas aeruginosa) in the microbiology laboratory. the study was designed to compare phenotypic with molecular identification. results of molecular analyses were compared with two commercially available identification systems (api 20 ne, vitek 2 fluorescent card; biomérieux, marcy l'etoil ... | 2006 | 16597863 |
| genetic and phenotypic microdiversity of ochrobactrum spp. | the diversity of ochrobactrum anthropi, ochrobactrum intermedium, ochrobactrum tritici and ochrobactrum grignonense in agricultural soil and on the wheat rhizoplane was investigated. o. anthropi was isolated both from soil and from the rhizoplane, o. intermedium and grignonense only from bulk soil, and o. tritici only from the wheat rhizoplane. on the genetic level, the immunotrapped isolates and a number of strains from culture collection mainly of clinical origin were compared with rep-pcr pro ... | 2006 | 16629756 |
| rapid detection of brucella spp. in blood cultures by fluorescence in situ hybridization. | brucellosis is a severe systemic disease in humans. we describe a new 16s rrna-based fluorescence in situ hybridization assay that facilitates rapid and specific detection of all human pathogenic species of brucella and that can be applied directly to positive blood cultures. | 2006 | 16672413 |
| evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting gram-negative rods. | to evaluate the activity of pyrrolidonyl arylamidase (pyr) for the differentiation and identification of nonfermenting gram negative rods (nfgnr), 293 isolates were tested. a 24 h culture of each test organism was prepared. from this a 108-109 cfu/ml suspension was added to 0.25 ml of sterile physiologic solution. a pyr disk was then added and the test was incubated for 30 minutes at 35-37 degrees c, at environmental atmosphere. reading was done by adding 1 drop of cinnamaldehyde reagent. strain ... | 2007 | 16822636 |
| ochrobactrum anthropi endocarditis and septic shock in a patient with no prosthetic valve or rheumatic heart disease: case report and review of the literature. | although ochrobactrum anthropi is an opportunistic pathogen in immunocompromised patients, it is increasingly being recognized to be a causative agent in healthy hosts. in this paper, we report a case of o. anthropi endocarditis and septic shock in a patient who had no prosthetic valve or rheumatic heart disease, in contrast to previous reports. | 2006 | 16936348 |