| 2-o-(beta-d-glucuronyl)-7-o-(2-amino-2-deoxy-alpha-d-glucopyranosyl)-l-glycero-d-manno-heptose: a constituent of the bordetella pertussis endotoxin. | the presence of bound d-glucuronic acid in the endotoxin of bordetella pertussis was demonstrated. the branched chain trisaccharide named in the title was isolated after hydrolysis of the endotoxin with 3 m hcl for 2 h at 100 degrees c. its structure was established by chemical and enzymic degradation. | 1977 | 196849 |
| identification of 2-methyl-3-hydroxydecanoic and 2-methyl-3-hydroxytetradecanoic acids in the 'lipid x' fraction of the bordetella pertussis endotoxin. | the 'lipid x' fraction, released from the bordetella pertussis endotoxin upon treatment with trifluoroacetic acid of ph 3 at 50 degrees c, was shown to contain, in addition to 3-hydroxydecanoic, 3-hydroxydodecanoic, 3-hydroxytetradecanoic, and tetradec-2-enoic acids, 2-methyl-3-hydroxydecanoic-, and 2-methyl-3-hydroxytetradecanoic acids. the structure of these was established by gas-liquid chromatography/mass spectrometry. | 1977 | 196857 |
| pertussis outbreak in austin and travis county, texas, 1975. | an outbreak of bacteriologically proven pertussis occurred in austin and travis county, texas, over a 7-month period in 1975. eighty persons were cultured for pertussis in our laboratory. a total of 62% of specimens from 34 individuals with suspected pertussis was positive for bordetella pertussis. diagnosis of acute cases by both culture and fluorescent antibody was attempted, and the correlation of the methods is given. analyses of cases by age, sex, immunization status, and antibiotic treatme ... | 1977 | 197117 |
| the mitogenic effect of the lymphocytosis promoting factor from bordetella pertussis on human lymphocytes. | the purified lymphocytosis promoting factor (lpf) from bordetella pertussis was found to be a potent mitogen for peripheral blood lymphocytes (pbl) from normal adults as well as for cord blood lymphocytes. proliferation occurred in autologous plasma or fetal calf serum, regardless of previous exposure to pertussis infection or immunization. only one adult human serum, from a physician constantly working with b. pertussis, inhibited the mitogenic response to lpf and this serum was shown to contai ... | 1977 | 197121 |
| vascular permeability changes in the central nervous system of rats with hyperacute experimental allergic encephalomyelitis induced with the aid of a substance from bordetella pertussis. | development of hyperacute experimental allergic encephalomyelitis in lewis rats after intraperitoneal administration of a mixture of guinea pig spinal cord emulsion and pertussigen from bordetella pertussis was accompanied by an increase in vascular permeability in the central nervous system. the increased permeability was most striking in the spinal cord and seemed to be associated with the ascending development of paralysis. rats that had completely recovered from paralysis did not have any in ... | 1978 | 211087 |
| pathogenesis of infection with bordetella pertussis in hamster tracheal organ culture. | hamster tracheal organ culture was employed as a model for study of the pathogenesis of infection due to bordetella pertussis. infected tracheal explants were examined with light, immunofluorescence, and electrom microscopy. b. pertussis organisms preferentially attached to the ciliated cells, producing ciliostasis and marked destruction of the subcellular organelles followed by expulsion of these cells from the epithelial layer. other nonciliated respiratory epithelial cells appeared to be unaf ... | 1977 | 197174 |
| lymphocytosis-promoting factor of bordetella pertussis: isolation, characterization, and biological activity. | the leukocytosis and lymphocytosis-promoting factor (lpf) of bordetella pertussis has been isolated in apparently pure form. lpf is a protein essentially free of lipid and carbohydrate with an estimated molecular weight of 67,000-73,600 daltons. purified lpf induced both histamine sensitization and refractoriness to epinephrine-induced hyperglycemia and was a murine thymus-derived (t-) cell mitogen. adenyl cyclase activity also appeared to be associated with lpf. | 1977 | 197175 |
| perfusion of the pancreas isolated from pertussis-sensitized rats: potentiation of insulin secretory responses due to beta-adrenergic stimulation. | in order to study the mechanism by which pertussis-sensitized rats showed enhanced insulin secretory responses to various secretagogues (sumi, t., and m. ui, endocrinology 97: 352, 1975), pancreases of rats receiving a single injection of bordetella pertussis cells 3 days before were perfused with krebs-ringer solution, and release of insulin therefrom was compared with that from the pancreases of normal rats. much more insulin was released from the pancreas of the pertussis-sensitized rat than ... | 1977 | 198199 |
| enrichment medium for the isolation of bordetella. | the development of a specimen collection and transport medium outfit for the rapid laboratory diagnosis of whoping cough is described. the transport medium consisted of a semisolid agar containing charcoal, cephalexin, and defibrinated horse blood. it was also found to be an excellent enrichment medium for the selective isolation of bordetella pertussis and b. parapertussis from scantily populated specimens. the investigation of 3,237 specimens that yielded 1,419 positive isolates of bordetella, ... | 1977 | 198430 |
| resistance of bordetella pertussis phase i to mucociliary clearance by rabbit tracheal mucous membrane. | cultivated mucous membrane of rabbit trachea was point inoculated with bordetella pertussis phase i or iii. phase i (virulent) bacteria were found to be infective at the point-inoculated site, but phase iii (avirulent) bacteria rarely showed such behavior. after inoculation, homogenized segments of mucous membrane were spread on plates. large numbers of phase i bacteria were recovered from the inoculated segment; however, the laryngeal segment was the site of recovery of large numbers of phase i ... | 1977 | 199673 |
| the influence of various methods of sensitization on delayed hypersensitivity to bordetella pertussis in the sprague dawley rat. | in the rat, the injection of bordetella pertussis produces, after prior sensitization, a delayed hypersensitivity inflammatory reaction within the pleural cavity. the influence of various methods of sensitization on this hypersensitivity was studied in the sprague dawley rat. the reaction was very variable according to the experimental conditions used. optimal sensitization was obtained following the injection of antigen mixed with complete freund's adjuvant into the dorsal surface of the paws. | 1977 | 200190 |
| oral feeding of ovalbumin can make rats tolerant to an intraperitoneal injection of dinitrophenylated ovalbumin and bordetella pertussis vaccine [proceedings]. | | 1977 | 200504 |
| [determination of major 1, 2, and 3 agglutinant antigens of bordetella pertussis strains isolated from brazilian children with whooping-cough treated at the emilio ribas hospital of são paulo, brazil]. | | 1977 | 203021 |
| pertussis vaccine--fact and fiction. | the present paper indicates a number of unresolved problems in the metabolism of bordetella pertussis and in the standardization of pertussis vaccines. the use of chemically defined media should enable the metabolic problems to be clarified. the problems concerned with the control and standardization of vaccines can largely be resolved in the laboratory with the introduction of reference preparations but the question of the reactivity of vaccine remains for the future. | 1977 | 203169 |
| bordetella pertussis in the national capital region: prevalent serotype and immunization status of patients. | over a 2-year period 67 strains of bordetella pertussis were identified in 231 single specimens of nasopharyngeal secretions submitted from patients suspected to have whooping cough in the national capital region; 89.5% of the identifications were made by culture. serotype 1,3 was predominant. at least 75% of the patients with bacteriologically confirmed whooping cough had not been fully immunized. there was no evidence that adenoviruses or other viruses played any important etiologic role in th ... | 1977 | 203375 |
| islets-activating protein (iap) in bordetella pertussis that potentiates insulin secretory responses of rats. purification and characterization. | | 1978 | 203574 |
| biological properties of islets-activating protein (iap) purified from the culture medium of bordetella pertussis. | the biological activities were studied of a new protein, islets-activating protein (iap), purified from the culture medium of bordetella pertussis. rats injected intravenously with 1 microgram of purified iap exhibited markedly enhanced insulin secretory responses to glucose, glucagon, epinephrine, and sulfonylureas over a period from 3 to 10 days after the injection. the degree and duration of the enhancement were proportional to the dose of iap; the maximal effect induced by 1-2 microgram of i ... | 1978 | 203575 |
| the role of antibiotics, immunizations, and adenoviruses in pertussis. | sixty-five patients with pertussis were identified by a clinical criterion, and bordetella pertussis was isolated from 75% of these patients or their symptomatic household contacts. negative nasopharyngeal cultures were usually associated with either a history of antibiotic therapy with erythromycin or tetracycline (two of three patients), two or more diphtheria and tetanus toxoids with pertussis (dtp) vaccines (six of eight patients), or both (two of three patients). erythromycin therapy result ... | 1978 | 204899 |
| reactions after pertussis vaccine: a manufacturer's experiences and difficulties since 1964. | pertussis vaccines vary in quality, safety, and efficacy according to the production strains of bordetella pertussis, the method of manufacture, and quality control procedures. it is therefore not justifiable to combine information on the incidence, nature, and severity of reactions after all manufacturers' pertussis vaccines as if they were a single product. attempts were made to collect information on all suspected cases of severe reactions that occurred after administration of about 15 millio ... | 1978 | 205305 |
| a novel type of endotoxin structure present in bordetella pertussis. isolation of two different polysaccharides bound to lipid a. | the endotoxin of bordetella pertussis was cleaved by mild acidic hydrolysis to yield a polysaccharide (polysaccharide i, 15%), a glycolipid (63%) and lipid x (2%). further treatment of the glycolipid with stronger acid released a second polysaccharide (polysaccharide ii, 9%) and material similar to lipid a present in enterobacterial endotoxins. both polysaccharides possess a single molecule of 3-deoxy-2-octulosonic acid as the reducing, terminal sugar. in polysaccharide ii the octulosonic acid i ... | 1978 | 205414 |
| role of complement in the pathogenesis of experimental autoimmune myasthenia gravis. | an acute phase of experimental autoimmune myasthenia gravis (eamg) occurs transiently early in the immune response of lewis rats to nicotinic acetylcholine receptors (achr) when bordetella pertussis is used as adjuvant. it is characterized by a destructive cellular attack directed at the postsynaptic membranes of muscle. acute eamg can be passively transferred to normal rats by igg from serum of rats with chronic eamg. in the present study, acute eamg, induced either by passive transfer of synge ... | 1978 | 206648 |
| [influence of killed bordetella pertussis cells on the resistance against infection with listeria monocytogenes (author's transl)]. | the influence of killed bordetella pertussis cells (b.p.) on the cell-mediated resistance of mice against infection with virulent germs of listeria monocytogenes has been studied. resistance of mice was decreased, when 3 x 10(9) b.p. were injected 1 day before, simultaneously with or 1 day after infection, resulting in augmented amounts of viable listeriae recovered from the spleens 3 days after infection (figure 1). the ld50 was strongly reduced (table 1). transfer of immune spleen cells to rec ... | 1978 | 207050 |
| modulation of the antibody response to sheep erythrocytes in murine spleen cell cultures by a t cell mitogen extracted from bordetella pertussis. | | 1978 | 207953 |
| regulation of bordetella pertussis extracytoplasmic adenylate cyclase. | | 1978 | 208400 |
| machakos project studies. agents affecting health of mother and child in a rural area of kenya. v. pertussis sentypes in kenyan children 1974--1975. | cultures of bordetella pertussis were isolated by pernasal swabs from kenyan children in whom whooping-cough was suspected. serotyping of 94 of these isolates was undertaken by four laboratories in europe, and there was very close agreement in their typing results. each laboratory found that the incidence of type 1, 3 was the lowest of the three types, and that there were approximately equal numbers of types 1, 2, 3 and 1, 2. no new serotype was found. this distribution of serotypes was found in ... | 1978 | 209590 |
| [antigenic activity of bordetella pertussis during cultivation in liquid nutrient media]. | the authors studied the antigenic activity of bordetella pertussis during stab cultivation. for the first time there were revealed changes (oscillations) of antigenic activity: formation of 3 peaks of which the middle was the highest. a possibility of improving the quality of pertussis vaccine by using biomass at the height of the peak was experimentally founded. | 1978 | 209647 |
| colony formation by subpopulations of human t lymphocytes. i. effects of phytohaemagglutinin and lymphocytosis-promoting factor from bordetella pertussis. | | 1978 | 217562 |
| antigens in penicillin allergy. iii. antigen and antibody levels in mice treated with pure and contaminated penicillins. | using a radioimmunoassay, it was shown that commercially available ampicillin preparations often contain penicilloylated high molecular weight impurities. these possess immunological activities and stimulate penicilloyl-specific antibody formation in mice treated according to a therapeutic schedule. using purified and experimentally contaminated preparations it was also found that exposure of the animals to escherichia coli and bordetella pertussis bacteria could increase the antibody formation ... | 1979 | 217840 |
| [proliferation processes in the spleen following administration of bordetella pertussis]. | | 1978 | 218390 |
| on the relationship between inflammation and altered camp metabolism in lungs of b pertussis-vaccinated mice. | bordetella pertussis-vaccinated mice were examined for evidence of inflammation. using polymorphonuclear leukocyte and fluid accumulation as markers, inflammation was evidenced in the lungs and to a lesser extent in the livers of such mice. both heart and kidney showed no evidence of inflammation. development of the inflammatory lesion followed a time course similar to that previously reported for increased sensitivity to histamine-mediated camp accumulation. this close parrallelism between infl ... | 1978 | 210126 |
| purification, characterization and biological activity of islets activating protein (iap) from the supernatant fluids of bordetella pertussis cultures [proceedings]. | | 1978 | 210305 |
| [induction of antibodies to native dna by components of bordetella pertussis]. | administration of bordetella pertussis and some of their components to mice induced an increase of dna-binding activity of the sera revealed under ionic strength conditions of physiological saline, mostly on the 14th day. it was shown by the inhibition method that interaction between mouse sera and native dna was specific. maximum increase in the quantity of mouse sera proteins reacting with dna under low ionic strength condition of physiological saline (0.05 m nacl) is revealed on the 7th day. ... | 1978 | 210865 |
| conversion of bordetella pertussis to bordetella parapertussis. | the epidemiological and drug susceptibility data on whooping cough suggested a possibility that bordetella pertussis converts in some way to bordetella parapertussis. to prove this, b. pertussis strain 75 was treated with n-methyl-n'-nitro-n-nitrosoguanidine and a mutant resistant to staphcillin v and eight mutants resistant to trimethoprim were isolated. the staphcillin v-resistant mutant of b. pertussis agreed with all of the criteria of b. parapertussis and the trimethoprim-resistant mutants ... | 1978 | 211161 |
| action of mannitol in various immunological experimental models. | the authors describe the inhibiting action of mannitol after repeated administration of low subcutaneous doses in a number of experimental immunological models. for example, in the rat it produces a reduction of the secondary arthritis of freund's adjuvant polyarthritis and also of the pleurisy due to bordetella pertussis hypersensitivity. in the mouse it reduces the reaction of delayed hypersensitivity to sheep red cells. its action is also marked against ovalbumin-induced active skin anaphylax ... | 1979 | 229721 |
| [comparative study of lipid a from pertussis microbes differing in the toxicity of their o-antigens. i. chemical composition and stability of the bond between lipid a and the specific polysaccharide in b. pertussis lipopolysaccharide]. | data presented in this work indicated that antigens, contrastive by toxicity, obtained by boiven's method and o'neill and tood's method from two strains of bordetella pertussis differed by stability of lipid a binding with the specific polysaccharide. the influence of duration of the lipopolysaccharide hydrolysis on the fatty acid content in lipid a, and of heptose in the specific polysaccharide was demonstrated. lipid a fatty acid composition was studied. it is supposed that bound fatty acids a ... | 1978 | 211760 |
| protection of mice against viral infection by corynebacterium parvum and bordetella pertussis. | mice could be significantly protected against infection with herpes simplex virus (hsv) by i.p. or i.v. injection of killed corynebacterium parvum 7 days before infection. this protection was seen in inbred strains of mice with a different degree of sensitivity to hsv and after both i.p. and i.v. infection. resistant mice immunosuppressed by x-irradiation and showing an increased susceptibility to hsv could also be protected by a previous injection of c. parvum. elevated levels of interferon wer ... | 1978 | 212522 |
| [morphological changes in the central nervous system of mice inoculated with a bordetella pertussis suspension]. | | 1978 | 212699 |
| bordetella pertussis serotypes in canada. | a study was done to determine the major antigenic factors of bordetella pertussis strains isolated throughout canada and whether these isolates have the same antigenic structure as the bacilli in the currently used vaccines. testing for the major pertussis antigens, factors 1, 2 and 3, was conducted with 440 freshly isolated strains of b. pertussis received from seven canadian provinces between august 1976 and february 1978 and six batches of pertussis vaccine or immunizing agents containing per ... | 1978 | 213182 |
| characterization of mouse peritoneal exudate and associated leukocyte adherence inhibitory activity after intraperitoneal injection of either bordetella pertussis or corynebacterium parvum vaccines. | bordetella pertussis and corynebacterium parvum are commonly used immunopotentiating agents. to explore the inflammatory environment induced by these agents, the peritoneal exudate response in mice following intraperitoneal injection of b. pertussis (pv) and c. parvum (cv) vaccines was investigated. the pv-induced exudate isolated by lavage was characterized by an early neutrophil influx followed by enhanced accumulation of mononuclear cells and fluid protein. the cv exudate was principally mono ... | 1978 | 215552 |
| effect of erythromycin and amoxycillin on bordetella pertussis in the nasopharynx. | the effectiveness of oral erythromycin and amoxycillin in eradicating bordetella pertussis from the nasopharynx was compared. erythromycin in a dosage of 40--50 mg/kg/day was significantly more effective than amoxycillin in a dosage of 25--30 mg/kg/day. the organism did not disappear in three cases receiving a lower dosage of erythromycin. as antibiotic treatment does not affect the clinical course of fully-developed whooping cough, erythromycin is indicated primarily when particularly susceptib ... | 1978 | 215555 |
| action of phenylbutazone, cyclophosphamide and prednacinolone on pleurisy due to bordetella pertussis hypersensitivity in the rat. | phenylbutazone, cyclophosphamide and prednacinolone acetonide, administered around the period of challenge reduced the exudate of pleurisy due to bordetella pertussis hypersensitivity in the rat. the results on the leukocytes of the exudate are different depending on the class of product studied. phenylbutazone only slightly reduced the number of mononuclears cells. both the immunosuppressive and the corticoid induced a clear decrease in the total leukocyte number. but, while prednacinolone effe ... | 1978 | 216327 |
| islet-activating protein. enhanced insulin secretion and cyclic amp accumulation in pancreatic islets due to activation of native calcium ionophores. | the mechanism whereby "islet-activating protein" (iap) purified from the culture medium of bordetella pertussis potentiates insulin secretion was studied by experiments in vitro with islets of rats once injected with iap (0.5 micrograms/100 g body weight, 3 days before killing) or with islets that had been exposed to iap (0.1 to 100 ng/ml) for 24 h. the iap treatment markedly enhanced insulin secretory responses and camp accumulation in islets, facilitated the efflux of 45ca through the cell mem ... | 1979 | 216676 |
| corynebacterium parvum as an adjuvant for trypanosoma cruzi epimastigote vaccines: a comparison with saponin and bordetella pertussis. | the effect was compared in cba mice of adding corynebacterium parvum, saponin, and bordetella pertussis to living or killed trypanosoma cruzi (y strain) epimastigote vaccines on the induction of protective immunity against subcutaneous (s.c.) challenge with blood trypomastigotes. the addition of c. parvum to a low dose of t. cruzi vaccine, which alone was non-protective, generated a greater degree of protection than did saponin or b. pertussis. c. parvum alone increased resistance to infection t ... | 1979 | 216967 |
| influence of vaccination with bordetella pertussis cells on haemopoiesis in sublethally irradiated mice and their radiation lethality. | | 1978 | 217243 |
| the serological diagnosis of whooping cough. | indirect haemagglutination (iha), agglutination and complement fixation tests (cft) for bordetella pertussis antibodies were compared on paired sera from 52 suspected cases of whooping cough and single sera from 83 children with no recent history of whooping cough. all three tests detected serotype antibodies 1, 2 and 3, but the iha test was the most sensitive; in seven cases it was the only test to show a rise in titre. it is recommended, particularly with vaccinated children, that the serologi ... | 1979 | 222838 |
| subcellular fractions for immunizing against pertussis. | differential centrifugation was used to prepare fractions from broken cells of bordetella pertussis strain 114. whole cells and several fractions were then assayed for potency and for safety. crude ribosomal fractions were uniformly protective. however, ribosomes purified by washing in high salt solution and recentrifugation were at least 40 fold less potent. protective antigen was found in the wash fluid. wash fluid was subjected to sds-polyacrylamide gel electrophoresis. no specific protein or ... | 1978 | 223910 |
| host immune responses after administration of inactivated venezuelan equine encephalomyelitis virus vaccines--iii. kinetics for neutralizing antibody immunoglobulin class responses in donors and adoptively-immunized recipients. | c57b16 mice were immunized with either live, attenuated tc-83 strain vee virus vaccine or formalin-inactivated vee vaccine combined with bordetella pertussis. the kinetics of specific donor and adoptively-immunized recipient anti-vee neutralizing antibody responses were studied. donor mice immunized with either live or inactivated vee virus vaccine combined with potent adjuvants develop specific anti-vee igm and igg responses as early as 7 days post-immunization. anti-vee igm antibody responses ... | 1979 | 318539 |
| polymorphonuclear leukocyte-inhibitory factor of bordetella pertussis. i. extraction and partial purification of phagocytosis- and chemotaxis-inhibitory activities. | a new factor that inhibited phagocytosis to opsonized targets and chemotaxis of pmn was extracted from b. pertussis cells, and named pmn-inhibitory factor (pif). cells in phase i produced 10 times more pif than those in phase iii, and like other phase i-associated components--the hemagglutinin, the histamine-sensitizing factor and agglutinogens--pif showed degenerative, phenotypic variation during in vitro culture of phase i bacteria. pif was partially purified by four steps, including adsorptio ... | 1978 | 224858 |
| the influence of different adjuvants on the production of igd and ige antibodies. | the aim of this study was to investigate the relationship between the synthesis of igd and ige antibodies upon the administration of antigen. different adjuvants were used in order to find the optimal way of inducing in rats the synthesis of igd and ige antibodies. ovalbumin was used as antigen. fair amounts of ige antibodies were produced using al(oh)3 or bordetella pertussis and al(oh)3 as adjuvant. only small quantities of ige antibodies were formed when either freund's complete adjuvant (fca ... | 1979 | 219761 |
| [changes in the proliferative response of human lymphocytes in vitro on exposure to subcellular components of bordetella pertussis]. | different components of b. pertussis were found to have a similar inhibitory effect on thymidine-3h incorporation caused by phytohemagglutinin (pha) in the culture of lymphocytes taken from donors immunized with tetanus toxoid. however, the same fractions of b. pertussis produced differential effects on the reaction of lymphocyte proliferation in response to tetanus toxoid: murein-"ontaining membranes enhanced thymidine-3h incorporation, rna-containing fractions reduced it and water-soluble comp ... | 1979 | 219921 |
| adjuvant for ige antibody and islet-activating protein in bordetella pertussis. | a new purified protein, with a molecular weight of 77,000, isolated from the supernatant of bordetella pertussis culture fluid and called islet-activating protein (iap), had adjuvant activity for ige antibody in addition to leukocytosis-promoting, histamine-sensitizing and islet-activating activities. none of the three subunits (f1, f2 and f3) was biologically active by itself. all of the four activities were recovered in the complexes of subunits f1.3 and f2.3. these results suggest that the ac ... | 1979 | 220196 |
| [search for new strains of bordetella pertussis for vaccine production]. | | 1978 | 220475 |
| a protein activator for the adenylate cyclase of bordetella pertussis. | the activity of bordetella pertussis extracytoplasmic adenylate cyclase is 100-fold higher in organisms grown on blood agar than in those grown in synthetic medium. this increase in activity is due to in vivo activation of the enzyme by a factor present in erythrocytes. activation also occurs in killed or disrupted organisms. the activator can be separated from heme proteins and has been purified approximately 100-fold from erythrocytes, yielding material of approximately 105,000 daltons. it is ... | 1979 | 221475 |
| the in vitro effects of bordetella pertussis lymphocytosis-promoting factor on murine lymphocytes. iii. b-cell dependence for t-cell proliferation. | the nature of the helper lymphocytes in lymphocytosis-promoting factor (lpf)-induced proliferation was explored. removal of macrophages from adherent splenocytes by either carbonyl-iron incubation or passage through sephadex g-10 columns did not affect their synergistic function. nor did cytolysis with thy-1.2 antiserum and complement. the helper cells were found to be surface immunoglobulin-positive (sig+) because they are retained by anti-ig columns, susceptible to lysis by rabbit anti-mouse i ... | 1979 | 221609 |
| chemical modification of crude timothy grass pollen extract. iii. the effect of glutaraldehyde-induced aggregation on antigenic and immunogenic properties. | timothy pollen extracts have been reacted with glutaraldehyde under conditions leading to different degrees of aggregation of the product. aggregation tends to enhance the previously demonstrated effects of glutaraldehyde in that reactivity with human ige antibody, and ability to induce ige antibody in the bordetella pertussis-treated rat, are further reduced. ability to induce igg antibody with specificity for unmodified extract is substantially retained in all aggregated products. | 1977 | 406207 |
| the in vitro effects of bordetella pertussis lymphocytosis-promoting factor on murine lymphocytes. iv. generation, characterization, and specificity of cytotoxic lymphocytes. | cytotoxic effector lymphocytes were induced in cultures of mouse spleen or lymph node cells by lymphocytosis promoting factor (lpf). the lpf-activated cytotoxic cells: (a) were not generated unless proliferation occurred; (b) sedimented in the lighter density fraction of a bovine serum albumin gradient; (c) were large, blast-like cells; and (d) were lysed by thy-1.2 antiserum plus complement and, therefore, were t cells. neither lpf alone nor supernates from stimulated cultures were cytotoxic. u ... | 1979 | 221613 |
| evaluation of a ribosomal vaccine against pertussis. | a crude ribosomal vaccine derived from bordetella pertussis administered to icr and n:nih (sw) strains of mice protected them effectively against a standardized intracranial challenge. the dose of vaccine that protected half the mice was less for n:nih (sw) than for icr mice and compared favorably with a killed reference vaccine. ribosomes prepared from bacteria ground with washed sea sand were more immunogenic than those obtained by rupture with alumina or with a braun homogenizer. the protecti ... | 1979 | 222684 |
| intracellular localization of the dermonecrotic toxin of bordetella pertussis. | localization of the heat-labile dermonecrotic toxin of bordetella pertussis strain 114 grown in chemically defined stainer-scholte medium was studied by using skin reaction in 4-day-old suckling mice as the assay for toxin. through log phase and into stationary phase of growth the toxin was cell associated and not detected in the culture supernatant. only about 4% of the activity present in a suspension of lysed cells was detected in a suspension of whole cells, and the dermonecrotic activity wa ... | 1979 | 227787 |
| effects of fatty acids on growth of bordetella pertussis in defined medium. | the effects of saturated and unsaturated fatty acids on the growth of bordetella pertussis strain 114 in defined medium were tested. individual fatty acids were found to be either inhibitory or stimulatory to growth, depending on concentration. myristic (c14), pentadecanoic (c15), and palmitic (c16) acids were the most inhibitory saturated fatty acids tested. b. pertussis 114 was extremely sensitive to the unsaturated fatty acids oleic (c18; cis-9), elaidic (c18; trans-9) and petroselinic (c18; ... | 1979 | 227923 |
| tangential flow filtration of bordetella pertussis submerse cultures. | a procedure is reported for the large scale separation of bordetella pertussis microorganisms from liquid culture media by tangential flow filtration (cross flow filtration) using anisotropic membranes with a cut-off limit of 1 x 10(6) daltons, and microporous membranes with a pore size of 0.22 micrometer. | 1979 | 228969 |
| a method for the induction of a prolonged elevated igm response without the formation of igg antibody, by injecting carrageenan-treated mice with multiple doses of sheep erythrocytes. | carrageenan, a known macrophage toxin and immunosuppressive agent, has been studied for its effect on antibody responses following single doses or multiple daily increasing doses of sheep red blood cells (srbc) injected intraperitoneally (i.p.) in male cba strain mice. results showed that lambda carrageenan administered prior to a single i.p. injection of srbc markedly suppressed both the igm and igg responses. however, when carrageenan-treated mice were injected with multiple daily increasing a ... | 1979 | 313964 |
| antibody-forming potential of lymph nodes in aged mice, with special reference to the influence of adjuvant. | the secondary antibody-forming potential of non-splenic lymphatic tissues during senescence was investigated in nmri/han mice, both at the cellular and humoral levels. the mean life span of conventionally reared nmri/han mice amounts to 19.86 months. after primary immunization of aged (20-month-old) nmri mice with 4 x 10(8) sheep erythrocytes (se) by the intraperitoneal (i.p.) route, the primary antibody-forming potential of both spleen and lymph nodes was significantly reduced, as compared to y ... | 1977 | 318586 |
| immune response in mice to hapten conjugated sepharose. | mice were injected intraperitoneally with sepharose 4b beads coupled with hapten nip, and their anti-nip response was studied by counting antibody forming cells and determining serum titers. mice responded well to doses of 0.7 ml of packed beads but 0.3 and 1.2 doses induced much weaker responses. anti-nip titers in recipients of 0.7 ml of the antigen lasted nearly constant for at least 7 weeks. both t cell status of the recipient and use of adjuvant had an effect on the response. antigen withou ... | 1977 | 320823 |
| islet activating protein (iap) derived from the culture supernatant fluid of bordetella pertussis: effect on spontaneous diabetic rats. | the early phase of insulin secretion to an oral glucose load was blunted in spontaneous diabetic rats. the blunted insulin secretion was associated with markedly impaired glucose tolerance. a single injection of the islet activating protein (iap), a protein derived from the culture medium of bordetella pertussis, into the spontaneous diabetic rats normalised glucose tolerance. the increase in insulin response to glucose was an important contributing factor to the improvement of glucose tolerance ... | 1978 | 348542 |
| effect of in vivo pretreatment of rats with a new protein purified from bordetella pertussis on in vitro secretion of insulin: role of calcium. | rats were injected once iv with an islet-activating protein (iap), a new protein purified from the culture medium of bordetella pertussis. three days later, their pancreases were studied in vitro for insulin secretory responses. as with pertussis vaccine, pretreatment of rats with iap was effective in enhancing insulin release from pancreas during perfusion or from islets during incubation in response to secretagogues such as glucose and glibenclamide. the alpha-adrenergic inhibition of insulin ... | 1979 | 376293 |
| [serotyping of recently isolated strains of bordetella pertussis. ii. disparity of agglutination titers of patients' sera between antigens prepared from the maeno and recently isolated strains (author's transl)]. | | 1979 | 231129 |
| effect of fresh guinea-pig serum on the toxicity of bordetella pertussis and bordetella parapertussis. | | 1979 | 231604 |
| isolation of the lymphocytosis promoting factor-haemagglutinin of bordetella pertussis by affinity chromatography. | the lymphocytosis promoting factor-haemagglutinin of bordetella pertussis was isolated from solutions obtained after cell disintegration by a novel affinity chromatographic method using an adsorbent composed of human haptoglobin covalently attached to a sepharose 4b matrix. the haemagglutinin was bound to the adsorbent at ph 6.5 and eluted by a stepwise change to a ph 10 buffer. a 300--600-fold purification of the haemagglutinin was achieved by this one-step process. the chemical and biological ... | 1979 | 232665 |
| immunological and histological reactions to respiratory administration of ribosomal bordetella pertussis vaccine in mice. | | 1979 | 232799 |
| action of various drugs with anti-inflammatory or anti-rheumatic properties on pleurisy due to bordetella pertussis hypersensitivity in the rat. | the authors studied various drugs with anti-inflammatory or antirheumatic properties on pleurisy due to bordetella pertussis hypersensitivity in the rat. the following compounds were studies according to various methods of treatment: indomethacin, phenylbutazone, cyclophosphamide, desonide, chloroquine, levamisole, d-penicillamine and sodium aurothiopropanol sulphonate. the action on the volume of pleural exudate and on cell events depended on the compounds and the conditions of treatment. only ... | 1979 | 232922 |
| pertussis toxin: the cause of the harmful effects and prolonged immunity of whooping cough. a hypothesis. | the nature of the pathogenesis and of the prolonged immunity of whooping cough has not been clearly defined. the literature of bordetella pertussis indicated that only the antigen that induces histamine sensitization, lymphocytosis, and other biological reactions in mice is the cause of the harmful effects and prolonged immunity of whooping cough. this antigen has the general characteristics of bacterial protein exotoxins that cause the harmful effects of infectious diseases such as diphtheria a ... | 1979 | 233166 |
| specific unresponsiveness to skin allografts in mice. iv. immunological reactivity of mice treated with liver extracts, bordetella pertussis, and antilymphocyte serum. | the strain-specific unresponsiveness to h-2 incompatible skin allografts induced by treatment of adult mice with single inoculations of donor strain liver extract and bordetella pertussis vaccine, as well as three doses of antilymphocyte serum, has been investigated by several in vivo and in vitro methods, with a view to elucidating it mechanism. lymphoid cells from mice with long surviving skin grafts were found to be reactive in graft-versus-host assays (as measured by splenomegaly or poplitea ... | 1975 | 235163 |
| experimental studies on the biological properties of bordetella pertussis. | | 1979 | 262507 |
| reaginic antibody production to ascaris suum allergen, asc-1. i. the function of glutaraldehyde-polymerized antigen in the induction of reaginic (ige) antibodies in the rat. | rats immunized with a purified ascaris suum allergen (asc-1) or with its dinitrophenylated derivate (dnp-asc-1) produced high levels of reaginic (ige) antibodies. a second injection of antigen given 30 days later did not result in an anamnestic ige antibody response. immunization of adult-thymectomized, lethally-irradiated and bone-marrow reconstituted (atxb) rats with soluble asc-1 or dnp-asc-1 failed to stimulate reaginic antibody production. the administration of glutaraldehyde-polymerized an ... | 1977 | 301862 |
| the effects of basic pancreatic trypsin inhibitor (bpti) incorporated in freund's complete adjuvant and in freund's incomplete adjuvant containing bordetella pertussis organisms (per). suppressive effect on cell mediated response to purified protein derivative and competing effect on antibody formation to per in guinea pigs. | | 1977 | 304499 |
| comparison of vaccination of mice and rats with haemophilus influenzae and bordetella pertussis as models of atopy. | 1. rats and mice were vaccinated with haemophilus influenzae in different vaccination schedules whereafter blood eosinophils were counted. in rats a single vaccination resulted in a dose-dependent effect on the blood eosinophil count in a pattern comparable with that after bordetella pertussis vaccination. in a long-term vaccination schedule (five times a week for 5 weeks) rats developed a constant eosinophilia. in mice a single vaccination resulted in an eosinopenia of a consistent pattern whic ... | 1979 | 311260 |
| effect of bordetella pertussis vaccine on the course of lymphocytic choriomeningitis (lcm) virus infection in suckling mice pretreated with dianhydrodulcitol (dad). | in earlier experiments bordetella pertussis vaccine was found to enhance and dianhydrodulcitol (dad) to weaken cellular immune response to intracerebral lcm virus infection in suckling mice. b. pertussis vaccine proved to inhibit the restrictive effect of dad produced on the immune response in mice when 2-to-4-days-old animals were pretreated with dad and subsequently, at the age of 16 to 18 days of life, treated with b. pertussis vaccine then infected with lcm virus. consequently, b. pertussis ... | 1979 | 539467 |
| [opsonizing activity of sera from animals immunized with pertussis vaccine]. | opsonizing activity of guinea pig blood serum containing mercaptoethanol-resistant pertussis antibodies was studied in vitro on a model of microorganism ingestion by the mononuclears of the guinea pig peritoneal exudate. there were revealed distinct differences in the serum activity depending on the phagocytosis object. the blood serum of hyperimmunized rabbits stimulated the ingestion of bordetella pertussis by mononuclears of guinea pigs--normal and immunized with pertussis vaccine. the blood ... | 1978 | 567917 |
| effect of histamine on respiratory movements and isolated ileum of pertussis-vaccinated mice. a negative study. | 1. the sensitivity of mice, of the swiss random bred strain, previously i.p. vaccinated with bordetella pertussis phase i cells, to histamine was investigated. 2. no response and no increase of the sensitivity of the respiratory system of the pertussis-vaccinated mice was observed during and after the inhaled histamine. 3. no contractions and no response of the isolated perfused ileum of the pertussis-vaccinated mice were observed after the addition of histamine. 4. no experimental or control mo ... | 1979 | 582991 |
| production of guinea pig reaginic antibody against the house dust mite extract, dermatophagoides pteronyssinus, without adjuvant. | guinea pigs of hartley strain were sensitized with an extract of the house dust mite, dermatophagoides pteronyssinus. positive immediate skin reactions were obtained in more than half of the animals 6 weeks after repeated intradermal injection. adjuvants of a1(oh)3 gel or bordetella pertussis vaccine did not significantly enhance the production of reaginic antibody. prausnitz-küstner type skin test titers were even higher in guinea pigs sensitized with the mite extract without adjuvant. circadia ... | 1977 | 599718 |
| formation of biologically active protein from the subunits of islets-activating protein (iap), a new protein isolated from bordetella pertussis. | based on the finding reported in the preceding paper (kanbayashi, et al.: j. biochem) that subunits of islets-activating protein (iap), a new protein purified from the culture media of bordetella pertussis, were inactive as such, but regained the original biological activities when recombined, the conditions required for recovery of the biological activities were studied. essentially the same biological activities as the native iap were recovered when the smallest subunit, f-3, was incubated wit ... | 1978 | 359541 |
| histamine hypersensitivity in mice induced by concanavalin a. | this study compared the responses of cfw and cfi mice to concanavalin a (con a) and the histamine-sensitizing factor (hsf) of bordetella pertussis. there were marked similarities between these two agents with regard to systems implicated in induced histamine sensitivity. con a, like hsf, induces the sensitivity in cfw but not in cfi mice. the sensitizing agents both require the same time for optimum sensitization, both induce cutaneous sensitivities to histamine, and the mice are protected from ... | 1978 | 621347 |
| tumour-associated inhibition of immediate hypersensitivity reactions in mice. | the intensity of anaphylactic shock was lower in c3h mice carrying a methylcholanthrene-induced tumour (mcc3) than in their normal counterparts when immunized with ovalbumin and challenged i.v. after 14 days. this tumour-associated inhibitory effect on active systemic anaphylaxis was exerted mainly on events occurring after homocytotropic antibody synthesis because the serum titres of these antibodies were comparable in normal and tumour-bearing animals. in addition, passive systemic anaphylacti ... | 1977 | 405315 |
| host immune responses after administration of inactivated venezuelan equine encephalomyelitis virus vaccines. i. description and characterization of adoptive transfer by immune spleen cells. | cellular immune responses after immunization with a number of inactivated venezuelan equine encephalomyelitis (vee) virus vaccines were evaluated in mice by means of an adoptive transfer system. formalin-inactivated, tc-83 strain vee virus vaccine was immunogenic and highly effective in protecting recipient mice against challenge with virulent vee virus. in contrast to immunization with live tc-83 vee virus vaccine, however, immunization with inactivated vee vaccine did not provide donor mice wi ... | 1976 | 939921 |
| a comparative study of the anti-allergic effects of disodium baicalein 6-phosphate (bps) and disodium cromoglycate (dscg). | a comparative study was carried out on the effects of a soluble derivative of baicalein, disodium baicalein 6-phosphate (bps) and disodium cromoglycate (dscg) on the immediate type allergic reactions. bps not only inhibited reaginic antibody-mediated reactions including antigen-induced mediator release from monkey lung, homologous pca in rats, and reaginic antibody-mediated degranulation of mast cell, but also non-reaginic antibody-mediated reactions such as mediator release from guinea pig lung ... | 1977 | 405519 |
| allergenicity of concanavalin a in mice. | concanavalin a (con a) is a potent allergen in certain strains of mice and in particularly the h-2kk mice, a/j, cba/h, and c3h/he. using a dose of 100 microgram, the subcutaneous route of injection was the most effective means of inducing high, persistent titers of t cell-dependent circulating anti-con a reagins without the addition of the classical ige adjuvants, aluminium hydroxide and bordetella pertussis vaccine. haptenated con a induced reagins with antihapten specificity. in the discussion ... | 1979 | 437908 |
| influence of mode of blood sampling on the immunoreactive insulin concentration in serum of bordetella pertussis-treated mice [proceedings]. | | 1979 | 454932 |
| [counterimmunoelectrophoresis in the diagnosis of bordetella pertussis]. | the serum of 50 patients between 25 days to 15 years old who were admitted with the diagnosis of pertussis syndrome were investigated for precipitating antibodies by means of discontinuous counterimmunolectrophoresis (cied). the presence of antibodies for bordetella pertussis antigen was shown in 28 cases. all samples were taken within the first 24/48 hours of admission and at convalescence. the technique is easy to carry out, quick and of low cost. this test offers an adequate and fast means to ... | 1979 | 486252 |
| comparative antitumor effects of corynebacterium parvum, bordetella pertussis, bacillus calmette-guérin, and levamisole alone or in combination with cyclophosphamide in the cad2 murine mammary adenocarcinoma system. | the antitumor efficacy of various immune stimulants [corynebacterium parvum, bordetella pertussis, and bacillus calmette-guérin (bcg)] and levamisole alone or in conjunction with cyclophosphamide (cy) was studied in the cad2 mammary adenocarcinoma system using schedules developed previously with c. parvum and cy. weekly systemic treatment with c. parvum, b. pertussis, or bcg was effective in controlling tumor growth and had equivalent antitumor effects, but weekly treatment (or a single treatmen ... | 1979 | 498111 |
| loss of adjuvanticity in rats for the hyperacute form of allergic encephalomyelitis and for reaginic antibody production in mice of a phenotypic variant of bordetella pertussis. | the adjuvanticity of a phenotypic (c-mode) variant of b. pertussis, known to be deficient in certain immunological and physiopathological properties, was compared to that of the normal (x-mode) strain. the x-mode vaccine was a potent adjuvant for induction of hyperacute experimental allergic encephalomyelitis to guinea-pig spinal cord in lewis rats whereas c-mode vaccine was inactive. x-mode vaccine was also highly active in the induction of reaginic (both ige and iggl) antibodies to ovalbumin i ... | 1979 | 500113 |
| effect of ascaris suum and other adjuvants on the potentiation of the ige response in guinea-pigs. | the potentiation effect of various adjuvants on the production of guinea-pig ige was investigated using freund's complete and incomplete adjuvant, the lipopolysaccharide of escherichia coli and salmonella typhosa, bordetella pertussis, and the nematodes nippostrongylus brasiliensis and ascaris suum. while all the antigens had a variable effect on the potential of the igg response, only infection with a. suum resulted in an enhanced ige response to the antigen, egg albumin. maximum potentiation o ... | 1979 | 500118 |
| reversion of dextran sulfate-induced loss of antibacterial resistance by bordetella pertussis. | parenteral injection of dextran sulfate 500 (ds 500; 50 mg/kg of body weight) into mice caused a complete loss of resistance to a sublethal (2 x 10(3) to 5 x 10(3)) infection with listeria monocytogenes. such loss could be prevented by pretreatment of animals with 3 x 10(9) heat-killed bordetella pertussis organisms (po) 5 to 30 days before the administration of ds 500. the increased phagocytic capcity induced by po was only exhausted when a fourfold dose of ds 500, effecting complete loss of an ... | 1978 | 640739 |
| the effects of d-pencillamine and levamisole on leucocyte chemotaxis in the rat. | the administration of d-penicillamine (25 mg/kg) or levamisole (5 mg/kg) had no effect on leucocyte emigration into the exudates formed in inert sponges implanted in normal rats. 2 in rats, previously sensitized to bordetella pertussis and implanted with sponges containing pertussis vaccine, an increased leucocyte migration into the exudates occurred; this was significantly enhanced by the administration of the drugs. 3 neither drug in vitro affected the chemotaxis of rat polymorphonuclear leuco ... | 1978 | 647156 |
| bordetella pertussis in the national capital region. | | 1978 | 647536 |
| acute hemolytic anemia related to diphtheria-pertussis-tetanus vaccination. | three infants developed severe hemolytic anemia following the second or third diphtheria-pertussistetanus vaccination. direct antiglobulin tests were positive, and the infant most severely affected also had reduced serum complement levels, indicating an immunological mechanism for the hemolysis. the presence of igm on the erythrocytes from 2 of the infants could be demonstrated by antiglobulin tests or immunization experiments. heat eluates of the erythrocytes from one of the infants contained a ... | 1978 | 654911 |
| [experimental asthma in rats, and the effect of n (3', 4'-dimethoxycinnamoyl) anthranilic acid (n-5') (author's transl)]. | although guinea pigs have been frequently used as a model of asthma, antibodies produced in this species are generally gamma1 and gamma2 and belong to igg. the antibody responsible for asthmatic attacks in humans is ige, and such is quite different from gamma1 and gamma2, immunologically. guinea pigs are not therefore an adequate model for investigating anti-asthmatic drugs which inhibit ige-mediated mediator release, such as disodium cromoglycate. on the other hand, rats do produce an antibody ... | 1978 | 711029 |
| course of lymphocytic choriomeningitis (lcm) virus infection in suckling mice treated with bordetella pertussis vaccine. | death occurred earlier and the mortality rate was higher in one and two-week-old mice pretreated with bordetella pertussis vaccine and infected intracerebrally with lcm virus, than in not pretreated animals of the same litter. pertussis vaccine treatment contributed to the course of lcm virus infection ending in lethal meningitis in suckling mice, by accelerating the development of their cellular immune response. | 1978 | 754519 |
| the deleterious effect of bordetella pertussis vaccine and poly(ri.rc) on the metabolism and disposition of phenytoin. | the elimination of phenytoin from blood was significantly decreased in rats pretreated 24 hr previously with bordetella pertussis vaccine or the synthetic polynucleotide poly(ri.rc.). phenytoin half-life was increased about 4-fold by both agents. in microsomes prepared from rats pretreated with b. pertussis vaccine or poly(ri.rc) the hydroxylation of phenytoin in vitro was decreased by 36 nad 53%, respectively. the addition of these agents to normal microsomal suspensions had no effect on phenyt ... | 1979 | 762660 |
| the effect of bacterial adjuvants on trypanosoma lewisi infections in rats. | the effect of escherichia coli endotoxin, b.c.g. vaccine and bordetella pertussis vaccine on the parasitemia of trypanosoma lewisi was studied in rats. it was found that in general, b. pertussis administration resulted in higher and more prolonged parasitemias than normal. in contrast e. coli endotoxin tended to reduce both the intensity and duration of parasitemia. possible reasons for this phenomenon are discussed. | 1975 | 770268 |
| elevated 7s immunoglobulin and acute phase proteins in adjuvant-injected chickens. | injection of chickens with fca or fia supplemented with either bordetella pertussis or nocardia rubra induced greatly increased serum levels of 7s ig and proteins with alpha-, beta-, and gamma-mobility in electrophoresis. the serum protein changes were correlated with the development of dhs and the formation of a large allergic granuloma. the 7s ig was considered "nonspecific" since it was not adsorbed with the bacterial cells. the alpha-, beta-, and gamma-mobility proteins were identified as ac ... | 1975 | 813235 |