| [antigenic activity of bordetella pertussis during cultivation in liquid nutrient media]. | the authors studied the antigenic activity of bordetella pertussis during stab cultivation. for the first time there were revealed changes (oscillations) of antigenic activity: formation of 3 peaks of which the middle was the highest. a possibility of improving the quality of pertussis vaccine by using biomass at the height of the peak was experimentally founded. | 1978 | 209647 |
| on the relationship between inflammation and altered camp metabolism in lungs of b pertussis-vaccinated mice. | bordetella pertussis-vaccinated mice were examined for evidence of inflammation. using polymorphonuclear leukocyte and fluid accumulation as markers, inflammation was evidenced in the lungs and to a lesser extent in the livers of such mice. both heart and kidney showed no evidence of inflammation. development of the inflammatory lesion followed a time course similar to that previously reported for increased sensitivity to histamine-mediated camp accumulation. this close parrallelism between infl ... | 1978 | 210126 |
| purification, characterization and biological activity of islets activating protein (iap) from the supernatant fluids of bordetella pertussis cultures [proceedings]. | | 1978 | 210305 |
| host immune responses after administration of inactivated venezuelan equine encephalomyelitis virus vaccines. ii. kinetics of neutralizing antibody responses in donors and adoptively immunized recipients. | lymphoid cell responses to immunization with various formalin-inactivated venezuelan equine encephalomyelitis (vee) virus vaccines were monitored in mice by assessment of the development of both the neutralizing antibody response in sera of spleen cell donors and the adoptive neutralizing antibody response induced by spleen cell transfer in recipients. donors immunized intraperitoneally with formalin-inactivated vee vaccine (a single dose or a dose on three consecutive days) developed early seru ... | 1976 | 181497 |
| vascular permeability changes in the central nervous system of rats with hyperacute experimental allergic encephalomyelitis induced with the aid of a substance from bordetella pertussis. | development of hyperacute experimental allergic encephalomyelitis in lewis rats after intraperitoneal administration of a mixture of guinea pig spinal cord emulsion and pertussigen from bordetella pertussis was accompanied by an increase in vascular permeability in the central nervous system. the increased permeability was most striking in the spinal cord and seemed to be associated with the ascending development of paralysis. rats that had completely recovered from paralysis did not have any in ... | 1978 | 211087 |
| conversion of bordetella pertussis to bordetella parapertussis. | the epidemiological and drug susceptibility data on whooping cough suggested a possibility that bordetella pertussis converts in some way to bordetella parapertussis. to prove this, b. pertussis strain 75 was treated with n-methyl-n'-nitro-n-nitrosoguanidine and a mutant resistant to staphcillin v and eight mutants resistant to trimethoprim were isolated. the staphcillin v-resistant mutant of b. pertussis agreed with all of the criteria of b. parapertussis and the trimethoprim-resistant mutants ... | 1978 | 211161 |
| [comparative study of lipid a from pertussis microbes differing in the toxicity of their o-antigens. i. chemical composition and stability of the bond between lipid a and the specific polysaccharide in b. pertussis lipopolysaccharide]. | data presented in this work indicated that antigens, contrastive by toxicity, obtained by boiven's method and o'neill and tood's method from two strains of bordetella pertussis differed by stability of lipid a binding with the specific polysaccharide. the influence of duration of the lipopolysaccharide hydrolysis on the fatty acid content in lipid a, and of heptose in the specific polysaccharide was demonstrated. lipid a fatty acid composition was studied. it is supposed that bound fatty acids a ... | 1978 | 211760 |
| protection of mice against viral infection by corynebacterium parvum and bordetella pertussis. | mice could be significantly protected against infection with herpes simplex virus (hsv) by i.p. or i.v. injection of killed corynebacterium parvum 7 days before infection. this protection was seen in inbred strains of mice with a different degree of sensitivity to hsv and after both i.p. and i.v. infection. resistant mice immunosuppressed by x-irradiation and showing an increased susceptibility to hsv could also be protected by a previous injection of c. parvum. elevated levels of interferon wer ... | 1978 | 212522 |
| [morphological changes in the central nervous system of mice inoculated with a bordetella pertussis suspension]. | | 1978 | 212699 |
| bordetella pertussis serotypes in canada. | a study was done to determine the major antigenic factors of bordetella pertussis strains isolated throughout canada and whether these isolates have the same antigenic structure as the bacilli in the currently used vaccines. testing for the major pertussis antigens, factors 1, 2 and 3, was conducted with 440 freshly isolated strains of b. pertussis received from seven canadian provinces between august 1976 and february 1978 and six batches of pertussis vaccine or immunizing agents containing per ... | 1978 | 213182 |
| production of circulating reaginic (ige) antibodies by oral administration of ovalbumin to rats. | reaginic antibody synthesis following parenteral and/or oral administration of ovalbumin and bordetella pertussis organisms as adjuvant has been evaluated in lou/m/wsl inbred rats. these rats are able to produce high reaginic antibody serum levels after intraperitoneal injection of this antigen. primary oral administration of ovalbumin doses between 10 and 100 mg with bordetella pertussis organisms given as adjuvant by the intraperitoneal or the oral route led to characteristic reagnic responses ... | 1976 | 179939 |
| effect of erythromycin and amoxycillin on bordetella pertussis in the nasopharynx. | the effectiveness of oral erythromycin and amoxycillin in eradicating bordetella pertussis from the nasopharynx was compared. erythromycin in a dosage of 40--50 mg/kg/day was significantly more effective than amoxycillin in a dosage of 25--30 mg/kg/day. the organism did not disappear in three cases receiving a lower dosage of erythromycin. as antibiotic treatment does not affect the clinical course of fully-developed whooping cough, erythromycin is indicated primarily when particularly susceptib ... | 1978 | 215555 |
| the effect of bordetella pertussis on rosette-forming cells present in the peritoneal fluids of mice. | | 1976 | 177778 |
| islet-activating protein. enhanced insulin secretion and cyclic amp accumulation in pancreatic islets due to activation of native calcium ionophores. | the mechanism whereby "islet-activating protein" (iap) purified from the culture medium of bordetella pertussis potentiates insulin secretion was studied by experiments in vitro with islets of rats once injected with iap (0.5 micrograms/100 g body weight, 3 days before killing) or with islets that had been exposed to iap (0.1 to 100 ng/ml) for 24 h. the iap treatment markedly enhanced insulin secretory responses and camp accumulation in islets, facilitated the efflux of 45ca through the cell mem ... | 1979 | 216676 |
| corynebacterium parvum as an adjuvant for trypanosoma cruzi epimastigote vaccines: a comparison with saponin and bordetella pertussis. | the effect was compared in cba mice of adding corynebacterium parvum, saponin, and bordetella pertussis to living or killed trypanosoma cruzi (y strain) epimastigote vaccines on the induction of protective immunity against subcutaneous (s.c.) challenge with blood trypomastigotes. the addition of c. parvum to a low dose of t. cruzi vaccine, which alone was non-protective, generated a greater degree of protection than did saponin or b. pertussis. c. parvum alone increased resistance to infection t ... | 1979 | 216967 |
| influence of vaccination with bordetella pertussis cells on haemopoiesis in sublethally irradiated mice and their radiation lethality. | | 1978 | 217243 |
| colony formation by subpopulations of human t lymphocytes. i. effects of phytohaemagglutinin and lymphocytosis-promoting factor from bordetella pertussis. | | 1978 | 217562 |
| antigens in penicillin allergy. iii. antigen and antibody levels in mice treated with pure and contaminated penicillins. | using a radioimmunoassay, it was shown that commercially available ampicillin preparations often contain penicilloylated high molecular weight impurities. these possess immunological activities and stimulate penicilloyl-specific antibody formation in mice treated according to a therapeutic schedule. using purified and experimentally contaminated preparations it was also found that exposure of the animals to escherichia coli and bordetella pertussis bacteria could increase the antibody formation ... | 1979 | 217840 |
| [proliferation processes in the spleen following administration of bordetella pertussis]. | | 1978 | 218390 |
| 3-deoxy-2-octulosonic acid 5-phosphate: a component of the endotoxin of bordetella pertussis. | upon hydrolysis with 2 n hydrochloric acid for 2 h, a 3-deoxy-octulosonic acid 5-phosphate was released from the endotoxin of bordetella pertussis. the structure of the compound was established through chemical degradation. by periodate treatment of the intact endotoxin it was shown that positions 7 and 8 of the bound octulosonic acid phosphate were free, which, if present in a cyclic form, must be a pyranoside. | 1975 | 173533 |
| [changes in the proliferative response of human lymphocytes in vitro on exposure to subcellular components of bordetella pertussis]. | different components of b. pertussis were found to have a similar inhibitory effect on thymidine-3h incorporation caused by phytohemagglutinin (pha) in the culture of lymphocytes taken from donors immunized with tetanus toxoid. however, the same fractions of b. pertussis produced differential effects on the reaction of lymphocyte proliferation in response to tetanus toxoid: murein-"ontaining membranes enhanced thymidine-3h incorporation, rna-containing fractions reduced it and water-soluble comp ... | 1979 | 219921 |
| adjuvant activity of the histamine-sensitizing factor of bordetella pertussis in different strains of mice. | the effect of an extract of histamine-sensitizing factor (hsf) of bordetella pertussis on the immune response of different strains of mice to ovalbumin (oa) was investigated with regard to optimal dose of antigen and adjuvant. it was observed that all strains of mice treated with hsf during immunization with oa demonstrated enhanced production of hemagglutinating antibodies, as compared to animals treated with antigen alone. this enhancement was generally not as great as that demonstrated when a ... | 1975 | 171224 |
| the use of spheroplast-derived strains to differentiate between bordetella pertussis heat-labile agglutinogens and protective antigen for mice. | | 1975 | 165202 |
| the use of bordetella pertussis preserved in liquid nitrogen as a challenge suspension in the kendrick mouse protection test. | | 1975 | 165201 |
| the in vitro effects of bordetella pertussis lymphocytosis-promoting factor on murine lymphocytes. iii. b-cell dependence for t-cell proliferation. | the nature of the helper lymphocytes in lymphocytosis-promoting factor (lpf)-induced proliferation was explored. removal of macrophages from adherent splenocytes by either carbonyl-iron incubation or passage through sephadex g-10 columns did not affect their synergistic function. nor did cytolysis with thy-1.2 antiserum and complement. the helper cells were found to be surface immunoglobulin-positive (sig+) because they are retained by anti-ig columns, susceptible to lysis by rabbit anti-mouse i ... | 1979 | 221609 |
| the mitogenic effect of the lymphocytosis promoting factor from bordetella pertussis on human lymphocytes. | the purified lymphocytosis promoting factor (lpf) from bordetella pertussis was found to be a potent mitogen for peripheral blood lymphocytes (pbl) from normal adults as well as for cord blood lymphocytes. proliferation occurred in autologous plasma or fetal calf serum, regardless of previous exposure to pertussis infection or immunization. only one adult human serum, from a physician constantly working with b. pertussis, inhibited the mitogenic response to lpf and this serum was shown to contai ... | 1977 | 197121 |
| 2-o-(beta-d-glucuronyl)-7-o-(2-amino-2-deoxy-alpha-d-glucopyranosyl)-l-glycero-d-manno-heptose: a constituent of the bordetella pertussis endotoxin. | the presence of bound d-glucuronic acid in the endotoxin of bordetella pertussis was demonstrated. the branched chain trisaccharide named in the title was isolated after hydrolysis of the endotoxin with 3 m hcl for 2 h at 100 degrees c. its structure was established by chemical and enzymic degradation. | 1977 | 196849 |
| the serological diagnosis of whooping cough. | indirect haemagglutination (iha), agglutination and complement fixation tests (cft) for bordetella pertussis antibodies were compared on paired sera from 52 suspected cases of whooping cough and single sera from 83 children with no recent history of whooping cough. all three tests detected serotype antibodies 1, 2 and 3, but the iha test was the most sensitive; in seven cases it was the only test to show a rise in titre. it is recommended, particularly with vaccinated children, that the serologi ... | 1979 | 222838 |
| subcellular fractions for immunizing against pertussis. | differential centrifugation was used to prepare fractions from broken cells of bordetella pertussis strain 114. whole cells and several fractions were then assayed for potency and for safety. crude ribosomal fractions were uniformly protective. however, ribosomes purified by washing in high salt solution and recentrifugation were at least 40 fold less potent. protective antigen was found in the wash fluid. wash fluid was subjected to sds-polyacrylamide gel electrophoresis. no specific protein or ... | 1978 | 223910 |
| [isolation and purification of a preparation possessing leukocytosis-stimulating properties from pertussis bacteria]. | a homogeneous protein lsf-2 preparation was extracted from the cultural fluid of bordetella pertussis strains of the 1.0.3 serological type by means of precipitation with ammonium sulphate and electrofocussing; this preparation proved to produce a marked leukocytosis-stimulating and a weak toxic action of delayed type in experiments on animals. intraperitoneal administration of 5 mug of the lsf-2 preparation caused a rise of leukocytosis in mice to 100,000 cells per 1 mm3, a delay in the gain in ... | 1977 | 190830 |
| study on toxicity of bordetella pertussis cultures and antigens. | the toxicity of 201 bordetella pertussis strains isolated in belgrade has been studied. the strains were grown on a modification of cohen and wheeler's medium. ld50 of examined strains ranging between 1.06 and 1.95 billion bacteria in 35 (17.41%), between 2.04 and 4.83 billion bacteria in 92 (45.77%) and between 5.31 and 7.79 billion bacteria in 46 (22.88%) were found. low toxic cultures of b. pertussis strains with ld50 ranging between 9.0 and 14.1 billion bacteria in 28 (13.43%) were obtained. ... | 1977 | 190070 |
| effects of fatty acids on growth of bordetella pertussis in defined medium. | the effects of saturated and unsaturated fatty acids on the growth of bordetella pertussis strain 114 in defined medium were tested. individual fatty acids were found to be either inhibitory or stimulatory to growth, depending on concentration. myristic (c14), pentadecanoic (c15), and palmitic (c16) acids were the most inhibitory saturated fatty acids tested. b. pertussis 114 was extremely sensitive to the unsaturated fatty acids oleic (c18; cis-9), elaidic (c18; trans-9) and petroselinic (c18; ... | 1979 | 227923 |
| tangential flow filtration of bordetella pertussis submerse cultures. | a procedure is reported for the large scale separation of bordetella pertussis microorganisms from liquid culture media by tangential flow filtration (cross flow filtration) using anisotropic membranes with a cut-off limit of 1 x 10(6) daltons, and microporous membranes with a pore size of 0.22 micrometer. | 1979 | 228969 |
| action of mannitol in various immunological experimental models. | the authors describe the inhibiting action of mannitol after repeated administration of low subcutaneous doses in a number of experimental immunological models. for example, in the rat it produces a reduction of the secondary arthritis of freund's adjuvant polyarthritis and also of the pleurisy due to bordetella pertussis hypersensitivity. in the mouse it reduces the reaction of delayed hypersensitivity to sheep red cells. its action is also marked against ovalbumin-induced active skin anaphylax ... | 1979 | 229721 |
| [serotyping of recently isolated strains of bordetella pertussis. ii. disparity of agglutination titers of patients' sera between antigens prepared from the maeno and recently isolated strains (author's transl)]. | | 1979 | 231129 |
| effect of fresh guinea-pig serum on the toxicity of bordetella pertussis and bordetella parapertussis. | | 1979 | 231604 |
| isolation of the lymphocytosis promoting factor-haemagglutinin of bordetella pertussis by affinity chromatography. | the lymphocytosis promoting factor-haemagglutinin of bordetella pertussis was isolated from solutions obtained after cell disintegration by a novel affinity chromatographic method using an adsorbent composed of human haptoglobin covalently attached to a sepharose 4b matrix. the haemagglutinin was bound to the adsorbent at ph 6.5 and eluted by a stepwise change to a ph 10 buffer. a 300--600-fold purification of the haemagglutinin was achieved by this one-step process. the chemical and biological ... | 1979 | 232665 |
| immunological and histological reactions to respiratory administration of ribosomal bordetella pertussis vaccine in mice. | | 1979 | 232799 |
| action of various drugs with anti-inflammatory or anti-rheumatic properties on pleurisy due to bordetella pertussis hypersensitivity in the rat. | the authors studied various drugs with anti-inflammatory or antirheumatic properties on pleurisy due to bordetella pertussis hypersensitivity in the rat. the following compounds were studies according to various methods of treatment: indomethacin, phenylbutazone, cyclophosphamide, desonide, chloroquine, levamisole, d-penicillamine and sodium aurothiopropanol sulphonate. the action on the volume of pleural exudate and on cell events depended on the compounds and the conditions of treatment. only ... | 1979 | 232922 |
| pertussis toxin: the cause of the harmful effects and prolonged immunity of whooping cough. a hypothesis. | the nature of the pathogenesis and of the prolonged immunity of whooping cough has not been clearly defined. the literature of bordetella pertussis indicated that only the antigen that induces histamine sensitization, lymphocytosis, and other biological reactions in mice is the cause of the harmful effects and prolonged immunity of whooping cough. this antigen has the general characteristics of bacterial protein exotoxins that cause the harmful effects of infectious diseases such as diphtheria a ... | 1979 | 233166 |
| 7-o-(2-amino-2-deoxy-alpha-d-glucopyranosyl)-l-glycero-d-manno-heptose. a constituent of the endotoxin of bordetella pertussis. | hydrolysis of the bordetella pertussis endotoxin, extracted from both "phase i" and "phase iv" bacteria, with 4 m hcl for 1 h at 100 degrees c, released the disaccharide named in the title; it was isolated by paper electrophoresis or by ion-exchange chromatography in about 1% yield (w/w). the structure of the heptose could be rigorously established by chemical degradation; the facts that the glucosaminidic linkage was hydrolysed by an enzyme preparation containing both, alpha and beta-n-acetylgl ... | 1976 | 188640 |
| experimental studies on the biological properties of bordetella pertussis. | | 1979 | 262507 |
| reaginic antibody production to ascaris suum allergen, asc-1. i. the function of glutaraldehyde-polymerized antigen in the induction of reaginic (ige) antibodies in the rat. | rats immunized with a purified ascaris suum allergen (asc-1) or with its dinitrophenylated derivate (dnp-asc-1) produced high levels of reaginic (ige) antibodies. a second injection of antigen given 30 days later did not result in an anamnestic ige antibody response. immunization of adult-thymectomized, lethally-irradiated and bone-marrow reconstituted (atxb) rats with soluble asc-1 or dnp-asc-1 failed to stimulate reaginic antibody production. the administration of glutaraldehyde-polymerized an ... | 1977 | 301862 |
| the effects of basic pancreatic trypsin inhibitor (bpti) incorporated in freund's complete adjuvant and in freund's incomplete adjuvant containing bordetella pertussis organisms (per). suppressive effect on cell mediated response to purified protein derivative and competing effect on antibody formation to per in guinea pigs. | | 1977 | 304499 |
| comparison of vaccination of mice and rats with haemophilus influenzae and bordetella pertussis as models of atopy. | 1. rats and mice were vaccinated with haemophilus influenzae in different vaccination schedules whereafter blood eosinophils were counted. in rats a single vaccination resulted in a dose-dependent effect on the blood eosinophil count in a pattern comparable with that after bordetella pertussis vaccination. in a long-term vaccination schedule (five times a week for 5 weeks) rats developed a constant eosinophilia. in mice a single vaccination resulted in an eosinopenia of a consistent pattern whic ... | 1979 | 311260 |
| a method for the induction of a prolonged elevated igm response without the formation of igg antibody, by injecting carrageenan-treated mice with multiple doses of sheep erythrocytes. | carrageenan, a known macrophage toxin and immunosuppressive agent, has been studied for its effect on antibody responses following single doses or multiple daily increasing doses of sheep red blood cells (srbc) injected intraperitoneally (i.p.) in male cba strain mice. results showed that lambda carrageenan administered prior to a single i.p. injection of srbc markedly suppressed both the igm and igg responses. however, when carrageenan-treated mice were injected with multiple daily increasing a ... | 1979 | 313964 |
| host immune responses after administration of inactivated venezuelan equine encephalomyelitis virus vaccines--iii. kinetics for neutralizing antibody immunoglobulin class responses in donors and adoptively-immunized recipients. | c57b16 mice were immunized with either live, attenuated tc-83 strain vee virus vaccine or formalin-inactivated vee vaccine combined with bordetella pertussis. the kinetics of specific donor and adoptively-immunized recipient anti-vee neutralizing antibody responses were studied. donor mice immunized with either live or inactivated vee virus vaccine combined with potent adjuvants develop specific anti-vee igm and igg responses as early as 7 days post-immunization. anti-vee igm antibody responses ... | 1979 | 318539 |
| antibody-forming potential of lymph nodes in aged mice, with special reference to the influence of adjuvant. | the secondary antibody-forming potential of non-splenic lymphatic tissues during senescence was investigated in nmri/han mice, both at the cellular and humoral levels. the mean life span of conventionally reared nmri/han mice amounts to 19.86 months. after primary immunization of aged (20-month-old) nmri mice with 4 x 10(8) sheep erythrocytes (se) by the intraperitoneal (i.p.) route, the primary antibody-forming potential of both spleen and lymph nodes was significantly reduced, as compared to y ... | 1977 | 318586 |
| cross-reactions between bordetella pertussis and twenty-eight other bacterial species. | cross-reactions between b. pertussis and 28 other bacterial species were studied by various quantitative immunoelectrophoretic methods. a sonicated b. pertussis antigen preparation and a corresponding pooled rabbit antiserum were used as reference system. two of the b. pertussis antigens were cross-reactive with antigens from 17, respectively 19, other bacterial species mainly gram-negative species. as judged by absorption of antibodies, the degree of cross-reactivity of these b. pertussis antig ... | 1976 | 187010 |
| islet activating protein (iap) derived from the culture supernatant fluid of bordetella pertussis: effect on spontaneous diabetic rats. | the early phase of insulin secretion to an oral glucose load was blunted in spontaneous diabetic rats. the blunted insulin secretion was associated with markedly impaired glucose tolerance. a single injection of the islet activating protein (iap), a protein derived from the culture medium of bordetella pertussis, into the spontaneous diabetic rats normalised glucose tolerance. the increase in insulin response to glucose was an important contributing factor to the improvement of glucose tolerance ... | 1978 | 348542 |
| formation of biologically active protein from the subunits of islets-activating protein (iap), a new protein isolated from bordetella pertussis. | based on the finding reported in the preceding paper (kanbayashi, et al.: j. biochem) that subunits of islets-activating protein (iap), a new protein purified from the culture media of bordetella pertussis, were inactive as such, but regained the original biological activities when recombined, the conditions required for recovery of the biological activities were studied. essentially the same biological activities as the native iap were recovered when the smallest subunit, f-3, was incubated wit ... | 1978 | 359541 |
| crossed immunoelectrophoretic analysis of bordetella pertussis antigens and of corresponding antibodies in human sera. | fourty-four antigens were demonstrated in sonicated preparations of bordetella pertussis (b.p.), using crossed immunoelectrophoresis against antiserum obtained from rabbits. no qualititative differences between the four strains of the danish pertussis vaccine were found. in preparations of b.p. culture medium, purified with respect to lymphocytosis promoting factor (lpf) activity, one antigen was possibly related to lpf. in human sera, antibodies against five of the b.p. antigens were demonstrat ... | 1976 | 187009 |
| machakos project studies. agents affecting health of mother and child in a rural area of kenya. v. pertussis sentypes in kenyan children 1974--1975. | cultures of bordetella pertussis were isolated by pernasal swabs from kenyan children in whom whooping-cough was suspected. serotyping of 94 of these isolates was undertaken by four laboratories in europe, and there was very close agreement in their typing results. each laboratory found that the incidence of type 1, 3 was the lowest of the three types, and that there were approximately equal numbers of types 1, 2, 3 and 1, 2. no new serotype was found. this distribution of serotypes was found in ... | 1978 | 209590 |
| a comparative study of the anti-allergic effects of disodium baicalein 6-phosphate (bps) and disodium cromoglycate (dscg). | a comparative study was carried out on the effects of a soluble derivative of baicalein, disodium baicalein 6-phosphate (bps) and disodium cromoglycate (dscg) on the immediate type allergic reactions. bps not only inhibited reaginic antibody-mediated reactions including antigen-induced mediator release from monkey lung, homologous pca in rats, and reaginic antibody-mediated degranulation of mast cell, but also non-reaginic antibody-mediated reactions such as mediator release from guinea pig lung ... | 1977 | 405519 |
| chemical modification of crude timothy grass pollen extract. iii. the effect of glutaraldehyde-induced aggregation on antigenic and immunogenic properties. | timothy pollen extracts have been reacted with glutaraldehyde under conditions leading to different degrees of aggregation of the product. aggregation tends to enhance the previously demonstrated effects of glutaraldehyde in that reactivity with human ige antibody, and ability to induce ige antibody in the bordetella pertussis-treated rat, are further reduced. ability to induce igg antibody with specificity for unmodified extract is substantially retained in all aggregated products. | 1977 | 406207 |
| allergenicity of concanavalin a in mice. | concanavalin a (con a) is a potent allergen in certain strains of mice and in particularly the h-2kk mice, a/j, cba/h, and c3h/he. using a dose of 100 microgram, the subcutaneous route of injection was the most effective means of inducing high, persistent titers of t cell-dependent circulating anti-con a reagins without the addition of the classical ige adjuvants, aluminium hydroxide and bordetella pertussis vaccine. haptenated con a induced reagins with antihapten specificity. in the discussion ... | 1979 | 437908 |
| influence of mode of blood sampling on the immunoreactive insulin concentration in serum of bordetella pertussis-treated mice [proceedings]. | | 1979 | 454932 |
| [induction of antibodies to native dna by components of bordetella pertussis]. | administration of bordetella pertussis and some of their components to mice induced an increase of dna-binding activity of the sera revealed under ionic strength conditions of physiological saline, mostly on the 14th day. it was shown by the inhibition method that interaction between mouse sera and native dna was specific. maximum increase in the quantity of mouse sera proteins reacting with dna under low ionic strength condition of physiological saline (0.05 m nacl) is revealed on the 7th day. ... | 1978 | 210865 |
| comparative antitumor effects of corynebacterium parvum, bordetella pertussis, bacillus calmette-guérin, and levamisole alone or in combination with cyclophosphamide in the cad2 murine mammary adenocarcinoma system. | the antitumor efficacy of various immune stimulants [corynebacterium parvum, bordetella pertussis, and bacillus calmette-guérin (bcg)] and levamisole alone or in conjunction with cyclophosphamide (cy) was studied in the cad2 mammary adenocarcinoma system using schedules developed previously with c. parvum and cy. weekly systemic treatment with c. parvum, b. pertussis, or bcg was effective in controlling tumor growth and had equivalent antitumor effects, but weekly treatment (or a single treatmen ... | 1979 | 498111 |
| loss of adjuvanticity in rats for the hyperacute form of allergic encephalomyelitis and for reaginic antibody production in mice of a phenotypic variant of bordetella pertussis. | the adjuvanticity of a phenotypic (c-mode) variant of b. pertussis, known to be deficient in certain immunological and physiopathological properties, was compared to that of the normal (x-mode) strain. the x-mode vaccine was a potent adjuvant for induction of hyperacute experimental allergic encephalomyelitis to guinea-pig spinal cord in lewis rats whereas c-mode vaccine was inactive. x-mode vaccine was also highly active in the induction of reaginic (both ige and iggl) antibodies to ovalbumin i ... | 1979 | 500113 |
| effect of ascaris suum and other adjuvants on the potentiation of the ige response in guinea-pigs. | the potentiation effect of various adjuvants on the production of guinea-pig ige was investigated using freund's complete and incomplete adjuvant, the lipopolysaccharide of escherichia coli and salmonella typhosa, bordetella pertussis, and the nematodes nippostrongylus brasiliensis and ascaris suum. while all the antigens had a variable effect on the potential of the igg response, only infection with a. suum resulted in an enhanced ige response to the antigen, egg albumin. maximum potentiation o ... | 1979 | 500118 |
| effect of bordetella pertussis vaccine on the course of lymphocytic choriomeningitis (lcm) virus infection in suckling mice pretreated with dianhydrodulcitol (dad). | in earlier experiments bordetella pertussis vaccine was found to enhance and dianhydrodulcitol (dad) to weaken cellular immune response to intracerebral lcm virus infection in suckling mice. b. pertussis vaccine proved to inhibit the restrictive effect of dad produced on the immune response in mice when 2-to-4-days-old animals were pretreated with dad and subsequently, at the age of 16 to 18 days of life, treated with b. pertussis vaccine then infected with lcm virus. consequently, b. pertussis ... | 1979 | 539467 |
| [opsonizing activity of sera from animals immunized with pertussis vaccine]. | opsonizing activity of guinea pig blood serum containing mercaptoethanol-resistant pertussis antibodies was studied in vitro on a model of microorganism ingestion by the mononuclears of the guinea pig peritoneal exudate. there were revealed distinct differences in the serum activity depending on the phagocytosis object. the blood serum of hyperimmunized rabbits stimulated the ingestion of bordetella pertussis by mononuclears of guinea pigs--normal and immunized with pertussis vaccine. the blood ... | 1978 | 567917 |
| characterization of mouse peritoneal exudate and associated leukocyte adherence inhibitory activity after intraperitoneal injection of either bordetella pertussis or corynebacterium parvum vaccines. | bordetella pertussis and corynebacterium parvum are commonly used immunopotentiating agents. to explore the inflammatory environment induced by these agents, the peritoneal exudate response in mice following intraperitoneal injection of b. pertussis (pv) and c. parvum (cv) vaccines was investigated. the pv-induced exudate isolated by lavage was characterized by an early neutrophil influx followed by enhanced accumulation of mononuclear cells and fluid protein. the cv exudate was principally mono ... | 1978 | 215552 |
| production of guinea pig reaginic antibody against the house dust mite extract, dermatophagoides pteronyssinus, without adjuvant. | guinea pigs of hartley strain were sensitized with an extract of the house dust mite, dermatophagoides pteronyssinus. positive immediate skin reactions were obtained in more than half of the animals 6 weeks after repeated intradermal injection. adjuvants of a1(oh)3 gel or bordetella pertussis vaccine did not significantly enhance the production of reaginic antibody. prausnitz-küstner type skin test titers were even higher in guinea pigs sensitized with the mite extract without adjuvant. circadia ... | 1977 | 599718 |
| histamine hypersensitivity in mice induced by concanavalin a. | this study compared the responses of cfw and cfi mice to concanavalin a (con a) and the histamine-sensitizing factor (hsf) of bordetella pertussis. there were marked similarities between these two agents with regard to systems implicated in induced histamine sensitivity. con a, like hsf, induces the sensitivity in cfw but not in cfi mice. the sensitizing agents both require the same time for optimum sensitization, both induce cutaneous sensitivities to histamine, and the mice are protected from ... | 1978 | 621347 |
| reversion of dextran sulfate-induced loss of antibacterial resistance by bordetella pertussis. | parenteral injection of dextran sulfate 500 (ds 500; 50 mg/kg of body weight) into mice caused a complete loss of resistance to a sublethal (2 x 10(3) to 5 x 10(3)) infection with listeria monocytogenes. such loss could be prevented by pretreatment of animals with 3 x 10(9) heat-killed bordetella pertussis organisms (po) 5 to 30 days before the administration of ds 500. the increased phagocytic capcity induced by po was only exhausted when a fourfold dose of ds 500, effecting complete loss of an ... | 1978 | 640739 |
| action of phenylbutazone, cyclophosphamide and prednacinolone on pleurisy due to bordetella pertussis hypersensitivity in the rat. | phenylbutazone, cyclophosphamide and prednacinolone acetonide, administered around the period of challenge reduced the exudate of pleurisy due to bordetella pertussis hypersensitivity in the rat. the results on the leukocytes of the exudate are different depending on the class of product studied. phenylbutazone only slightly reduced the number of mononuclears cells. both the immunosuppressive and the corticoid induced a clear decrease in the total leukocyte number. but, while prednacinolone effe ... | 1978 | 216327 |
| bordetella pertussis in the national capital region. | | 1978 | 647536 |
| perfusion of the pancreas isolated from pertussis-sensitized rats: potentiation of insulin secretory responses due to beta-adrenergic stimulation. | in order to study the mechanism by which pertussis-sensitized rats showed enhanced insulin secretory responses to various secretagogues (sumi, t., and m. ui, endocrinology 97: 352, 1975), pancreases of rats receiving a single injection of bordetella pertussis cells 3 days before were perfused with krebs-ringer solution, and release of insulin therefrom was compared with that from the pancreases of normal rats. much more insulin was released from the pancreas of the pertussis-sensitized rat than ... | 1977 | 198199 |
| [experimental asthma in rats, and the effect of n (3', 4'-dimethoxycinnamoyl) anthranilic acid (n-5') (author's transl)]. | although guinea pigs have been frequently used as a model of asthma, antibodies produced in this species are generally gamma1 and gamma2 and belong to igg. the antibody responsible for asthmatic attacks in humans is ige, and such is quite different from gamma1 and gamma2, immunologically. guinea pigs are not therefore an adequate model for investigating anti-asthmatic drugs which inhibit ige-mediated mediator release, such as disodium cromoglycate. on the other hand, rats do produce an antibody ... | 1978 | 711029 |
| course of lymphocytic choriomeningitis (lcm) virus infection in suckling mice treated with bordetella pertussis vaccine. | death occurred earlier and the mortality rate was higher in one and two-week-old mice pretreated with bordetella pertussis vaccine and infected intracerebrally with lcm virus, than in not pretreated animals of the same litter. pertussis vaccine treatment contributed to the course of lcm virus infection ending in lethal meningitis in suckling mice, by accelerating the development of their cellular immune response. | 1978 | 754519 |
| enrichment medium for the isolation of bordetella. | the development of a specimen collection and transport medium outfit for the rapid laboratory diagnosis of whoping cough is described. the transport medium consisted of a semisolid agar containing charcoal, cephalexin, and defibrinated horse blood. it was also found to be an excellent enrichment medium for the selective isolation of bordetella pertussis and b. parapertussis from scantily populated specimens. the investigation of 3,237 specimens that yielded 1,419 positive isolates of bordetella, ... | 1977 | 198430 |
| the effect of bacterial adjuvants on trypanosoma lewisi infections in rats. | the effect of escherichia coli endotoxin, b.c.g. vaccine and bordetella pertussis vaccine on the parasitemia of trypanosoma lewisi was studied in rats. it was found that in general, b. pertussis administration resulted in higher and more prolonged parasitemias than normal. in contrast e. coli endotoxin tended to reduce both the intensity and duration of parasitemia. possible reasons for this phenomenon are discussed. | 1975 | 770268 |
| elevated 7s immunoglobulin and acute phase proteins in adjuvant-injected chickens. | injection of chickens with fca or fia supplemented with either bordetella pertussis or nocardia rubra induced greatly increased serum levels of 7s ig and proteins with alpha-, beta-, and gamma-mobility in electrophoresis. the serum protein changes were correlated with the development of dhs and the formation of a large allergic granuloma. the 7s ig was considered "nonspecific" since it was not adsorbed with the bacterial cells. the alpha-, beta-, and gamma-mobility proteins were identified as ac ... | 1975 | 813235 |
| changes in bovine immunoglobulin levels during a response to homologous erythrocyte membrane antigen. | changes in serum immunoglobulin levels of igg1, igg2 and igm were studied in cows immunised with a butanol extract of bovine erythrocytes. donor and recipients were matched to differ at only one known red cell antigen, the v antigen, and after the first course of injections a weak antibody response to v antigen was associated with elevated levels of igg2 immunoglobulins. a second course of injections of v antigens together with bordetella pertussis led to a stronger and more longlasting antibody ... | 1976 | 823619 |
| effects of histamine-sensitizing factor and cortisol on lymphocyte adenyl cyclase responses. | levels of cyclic adenosime monophosphate (camp) in lymphocytes are regulated by beta-adrenergic agonists and pge1. the effect of these agonists is potentiated by cortisol. incubation of lymphocytes with histamine-sensitizing factor (hsf) leads to loss of response to epinephrine and pge1. the response can be partially restored by cortisol. incubation of lymphocytes with beta-adrenergic antagonists such as propranolol leads to the loss of beta-adrenergic agents but not pge1. the inhibition by prop ... | 1977 | 833377 |
| resistance of bordetella pertussis phase i to mucociliary clearance by rabbit tracheal mucous membrane. | cultivated mucous membrane of rabbit trachea was point inoculated with bordetella pertussis phase i or iii. phase i (virulent) bacteria were found to be infective at the point-inoculated site, but phase iii (avirulent) bacteria rarely showed such behavior. after inoculation, homogenized segments of mucous membrane were spread on plates. large numbers of phase i bacteria were recovered from the inoculated segment; however, the laryngeal segment was the site of recovery of large numbers of phase i ... | 1977 | 199673 |
| oral feeding of ovalbumin can make rats tolerant to an intraperitoneal injection of dinitrophenylated ovalbumin and bordetella pertussis vaccine [proceedings]. | | 1977 | 200504 |
| erythromycin prophylaxis for pertussis. | a neonate admitted to the newborn nursery was found to have bacteriologically proven bordetella pertussis whooping cough, and two of seven infants exposed to this child became infected. erythromycin administration to the seven infants apparently prevented further infection as well as the appearance of clinical disease in the two infected infants. this limited experience supports previous reports of the efficacy of erythromycin in preventing b. pertussis infection and disease in susceptible child ... | 1977 | 850603 |
| scanning electron microscopic study of hamster tracheal organ cultures infected with bordetella pertussis. | hamster tracheal organculture was employed as a model for the study of the pathogenesis of infection due to bordetella pertusis. scanning electron microscopy provided a three-dimensional view of the surface infection of the tracheal explants. phase i b. pertussis attached only to the ciliated epithelial cells, and a sequence of events involving the injury, expulsion, and destruction of these differentiated cells occurred. this in vitro model provides insights into the mechanisms by which b. pert ... | 1977 | 200679 |
| pertussis vaccine--fact and fiction. | the present paper indicates a number of unresolved problems in the metabolism of bordetella pertussis and in the standardization of pertussis vaccines. the use of chemically defined media should enable the metabolic problems to be clarified. the problems concerned with the control and standardization of vaccines can largely be resolved in the laboratory with the introduction of reference preparations but the question of the reactivity of vaccine remains for the future. | 1977 | 203169 |
| bordetella pertussis in the national capital region: prevalent serotype and immunization status of patients. | over a 2-year period 67 strains of bordetella pertussis were identified in 231 single specimens of nasopharyngeal secretions submitted from patients suspected to have whooping cough in the national capital region; 89.5% of the identifications were made by culture. serotype 1,3 was predominant. at least 75% of the patients with bacteriologically confirmed whooping cough had not been fully immunized. there was no evidence that adenoviruses or other viruses played any important etiologic role in th ... | 1977 | 203375 |
| host immune responses after administration of inactivated venezuelan equine encephalomyelitis virus vaccines. i. description and characterization of adoptive transfer by immune spleen cells. | cellular immune responses after immunization with a number of inactivated venezuelan equine encephalomyelitis (vee) virus vaccines were evaluated in mice by means of an adoptive transfer system. formalin-inactivated, tc-83 strain vee virus vaccine was immunogenic and highly effective in protecting recipient mice against challenge with virulent vee virus. in contrast to immunization with live tc-83 vee virus vaccine, however, immunization with inactivated vee vaccine did not provide donor mice wi ... | 1976 | 939921 |
| induction and suppression of reagins in the neonatal mouse. | neonatal swr mice are capable of synthesizing reagins when immunized with a mixture of concanavalin and ovalbumin or a mixture of bordetella pertussis, alum and ovalbumin. reaginic antibody-forming cells can be found in the spleen, lymph nodes, bone marrow and peyer's patches. tolerance with respect to ige can be induced by the injection of deaggregated ovalbumin into neonatal mice. | 1976 | 945227 |
| the role of antibiotics, immunizations, and adenoviruses in pertussis. | sixty-five patients with pertussis were identified by a clinical criterion, and bordetella pertussis was isolated from 75% of these patients or their symptomatic household contacts. negative nasopharyngeal cultures were usually associated with either a history of antibiotic therapy with erythromycin or tetracycline (two of three patients), two or more diphtheria and tetanus toxoids with pertussis (dtp) vaccines (six of eight patients), or both (two of three patients). erythromycin therapy result ... | 1978 | 204899 |
| role of complement in the pathogenesis of experimental autoimmune myasthenia gravis. | an acute phase of experimental autoimmune myasthenia gravis (eamg) occurs transiently early in the immune response of lewis rats to nicotinic acetylcholine receptors (achr) when bordetella pertussis is used as adjuvant. it is characterized by a destructive cellular attack directed at the postsynaptic membranes of muscle. acute eamg can be passively transferred to normal rats by igg from serum of rats with chronic eamg. in the present study, acute eamg, induced either by passive transfer of synge ... | 1978 | 206648 |
| evaluation of a ribosomal vaccine against pertussis. | a crude ribosomal vaccine derived from bordetella pertussis administered to icr and n:nih (sw) strains of mice protected them effectively against a standardized intracranial challenge. the dose of vaccine that protected half the mice was less for n:nih (sw) than for icr mice and compared favorably with a killed reference vaccine. ribosomes prepared from bacteria ground with washed sea sand were more immunogenic than those obtained by rupture with alumina or with a braun homogenizer. the protecti ... | 1979 | 222684 |
| polymorphonuclear leukocyte-inhibitory factor of bordetella pertussis. i. extraction and partial purification of phagocytosis- and chemotaxis-inhibitory activities. | a new factor that inhibited phagocytosis to opsonized targets and chemotaxis of pmn was extracted from b. pertussis cells, and named pmn-inhibitory factor (pif). cells in phase i produced 10 times more pif than those in phase iii, and like other phase i-associated components--the hemagglutinin, the histamine-sensitizing factor and agglutinogens--pif showed degenerative, phenotypic variation during in vitro culture of phase i bacteria. pif was partially purified by four steps, including adsorptio ... | 1978 | 224858 |
| intracellular localization of the dermonecrotic toxin of bordetella pertussis. | localization of the heat-labile dermonecrotic toxin of bordetella pertussis strain 114 grown in chemically defined stainer-scholte medium was studied by using skin reaction in 4-day-old suckling mice as the assay for toxin. through log phase and into stationary phase of growth the toxin was cell associated and not detected in the culture supernatant. only about 4% of the activity present in a suspension of lysed cells was detected in a suspension of whole cells, and the dermonecrotic activity wa ... | 1979 | 227787 |
| specific unresponsiveness to skin allografts in mice. iv. immunological reactivity of mice treated with liver extracts, bordetella pertussis, and antilymphocyte serum. | the strain-specific unresponsiveness to h-2 incompatible skin allografts induced by treatment of adult mice with single inoculations of donor strain liver extract and bordetella pertussis vaccine, as well as three doses of antilymphocyte serum, has been investigated by several in vivo and in vitro methods, with a view to elucidating it mechanism. lymphoid cells from mice with long surviving skin grafts were found to be reactive in graft-versus-host assays (as measured by splenomegaly or poplitea ... | 1975 | 235163 |
| immune response in mice to hapten conjugated sepharose. | mice were injected intraperitoneally with sepharose 4b beads coupled with hapten nip, and their anti-nip response was studied by counting antibody forming cells and determining serum titers. mice responded well to doses of 0.7 ml of packed beads but 0.3 and 1.2 doses induced much weaker responses. anti-nip titers in recipients of 0.7 ml of the antigen lasted nearly constant for at least 7 weeks. both t cell status of the recipient and use of adjuvant had an effect on the response. antigen withou ... | 1977 | 320823 |
| effect of in vivo pretreatment of rats with a new protein purified from bordetella pertussis on in vitro secretion of insulin: role of calcium. | rats were injected once iv with an islet-activating protein (iap), a new protein purified from the culture medium of bordetella pertussis. three days later, their pancreases were studied in vitro for insulin secretory responses. as with pertussis vaccine, pretreatment of rats with iap was effective in enhancing insulin release from pancreas during perfusion or from islets during incubation in response to secretagogues such as glucose and glibenclamide. the alpha-adrenergic inhibition of insulin ... | 1979 | 376293 |
| adjuvant effect of bordetella pertussis vaccine to sheep erythrocytes: enhancement of antibody formation by using subcutaneous administration of adjuvant and antigen. | the subcutaneous route (s.c.) was used to study the adjuvant effect of bordetella pertussis vaccine (pv) on the primary antibody response to sheep erythrocytes. the reasons for using the s.c. route are discussed. pv, besides enhancing the hemagglutinin response, also markedly increased the number of plaque-forming cells in the draining lymph nodes. a heated preparation of pv was tested and found to possess significant adjuvant activity. interestingly, the enhancement occurred in the absence of m ... | 1975 | 1104487 |
| tumour-associated inhibition of immediate hypersensitivity reactions in mice. | the intensity of anaphylactic shock was lower in c3h mice carrying a methylcholanthrene-induced tumour (mcc3) than in their normal counterparts when immunized with ovalbumin and challenged i.v. after 14 days. this tumour-associated inhibitory effect on active systemic anaphylaxis was exerted mainly on events occurring after homocytotropic antibody synthesis because the serum titres of these antibodies were comparable in normal and tumour-bearing animals. in addition, passive systemic anaphylacti ... | 1977 | 405315 |
| [leukocytes and antibody activity in lung infection by bordetella pertussis in mice]. | | 1975 | 1240236 |
| mechanism of reappearance of contact sensitivity to dncb in desensitized guinea pigs. | the different immunologic activities of lymph node and spleen cells as compared to peritoneal exudate cells were studied in temporarily desensitized, dncb-contact-sensitive guinea pigs. lymph node (and spleen) lymphocytes: (a) transfer contact sensitivity to syngeneic recipients when harvested at the time of unresponsiveness; (b) are only temporarily inactivated in tolerant recipients by the desensitizing procedure; (c) enter the lymph node (or spleen) compartments in a higher proportion than pe ... | 1976 | 1245756 |
| sensitizing capacity of syngeneic, allogeneic and xenogeneic ocular tissue. effect on intact and injured rat eyes. | experimental allergic uveoretinitis could not be induced by the following procedures in a total of 87 rats of four different inbred strains; intramuscular or intraperitoneal immunization with syngeneic, allogeneic or xenogeneic native ocular tissue (up to 80 eyes, treatment up to 15 months); intramuscular and subcutaneous application of complete freund's adjuvants and bordetella pertussis vaccine followed by perforation (induction of uveal and lenticular lesions) of the left eye. | 1976 | 1250241 |
| [counterimmunoelectrophoresis in the diagnosis of bordetella pertussis]. | the serum of 50 patients between 25 days to 15 years old who were admitted with the diagnosis of pertussis syndrome were investigated for precipitating antibodies by means of discontinuous counterimmunolectrophoresis (cied). the presence of antibodies for bordetella pertussis antigen was shown in 28 cases. all samples were taken within the first 24/48 hours of admission and at convalescence. the technique is easy to carry out, quick and of low cost. this test offers an adequate and fast means to ... | 1979 | 486252 |
| absolute configuration of 3-hydroxy fatty acids present in lipopolysaccharides from various bacterial groups. | the absolute configuration of 3-hydroxy fatty acids has been studied, which are present in the lipopolysaccharides of the following bacteria: phodopseudomonas gelatinosa, rh. viridis, rhodospirillum tenue, chromobacterium violaceum, pseudomonas aeruginosa, bordetella pertussis, vibrio metchnikovii, vibrio cholerae, salmonella spp., escherichia coli, shigella flexneri, proteus mirabilis, yersinia enterocolitica and fusobacterium nucleatum. the 3-hydroxy acids were liberated by strong alkaline hyd ... | 1976 | 1278168 |