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assessing genetic structure, diversity of bacterial aerosol from aeration system in an oxidation ditch wastewater treatment plant by culture methods and bio-molecular tools.airborne bacteria emissions from oxidation ditch with rotating aeration brushes were investigated in a municipal wastewater treatment plant in beijing, china. microbial samples were collected at different distances from the rotating brushes, different heights above the water surface, and different operation state over a 3-month period (april, may, and june) in order to estimate the seasonal variation and site-related distribution characteristics of the microorganisms present. the concentration o ...201322402990
myroides infection in a baboon after prolonged pig kidney graft survival.immunosuppressed patients and experimental nonhuman primates are at risk of opportunistic infection. we report a myroides spp infection in an immunosuppressed baboon that had received a life-supporting kidney from a genetically-engineered pig.201526146660
detection of extended spectrum beta-lactamases resistance genes among bacteria isolated from selected drinking water distribution channels in southwestern nigeria.extended spectrum beta-lactamases (esbl) provide high level resistance to beta-lactam antibiotics among bacteria. in this study, previously described multidrug resistant bacteria from raw, treated, and municipal taps of dwds from selected dams in southwestern nigeria were assessed for the presence of esbl resistance genes which include bla tem, bla shv, and bla ctx by pcr amplification. a total of 164 bacteria spread across treated (33), raw (66), and municipal taps (68), belonging to α-proteoba ...201627563674
metal content of metallo-beta-lactamase l1 is determined by the bioavailability of metal ions.in an effort to probe whether the metal content of metallo-beta-lactamase l1 is affected by metal ion bioavailability, l1 was overexpressed as mature protein (m-l1) and full-length (fl-l1) analogues, and the analogues were characterized with metal analyses, kinetics, and epr spectroscopy. fl-l1, containing the putative leader sequence, was localized in the periplasm of escherichia coli and shown to bind zn(ii) preferentially. the metal content of fl-l1 could be altered if the enzyme was overexpr ...200818597493
myroides injenensis sp. nov., a new member isolated from human urine.a gram-negative, yellow-pigmented, rod-shaped bacteria, designated m09-0166(t)and m09-1053 were isolated from human urine samples. 16s rrna gene sequence analysis revealed that the isolates belong to the myroides cluster and were closely related to myroides phaeus dsm 23313(t) (96.3 %), myroides odoratimimus kctc 23053(t) (96.1 %), myroides profundi kctc 23066(t) (96.0 %), myroides odoratus kctc 23054(t) (95.4 %) and myroides pelagicus kctc 12661(t) (95.2 %). the major mena quinone was identifie ...201525515411
persistence of bacterial indicators and zoonotic pathogens in contaminated cattle wastes.manure can provide a favourable environment for pathogens' survival. de-contamination may be conducted by extended storage, until field conditions are suitable for application to land as source of agricultural nutrients.201627206734
antibiotic resistance mechanisms of myroides sp.bacteria of the genus myroides (myroides spp.) are rare opportunistic pathogens. myroides sp. infections have been reported mainly in china. myroides sp. is highly resistant to most available antibiotics, but the resistance mechanisms are not fully elucidated. current strain identification methods based on biochemical traits are unable to identify strains accurately at the species level. while 16s ribosomal rna (rrna) gene sequencing can accurately achieve this, it fails to give information on t ...201626984839
fatal case of necrotizing fasciitis due to myroides odoratus.myroides sp., previously known as flavobacterium odoratum, is a relatively unknown organism with unclear human pathogenicity. while myroides sp. has been implicated in human infections, many reports have described the organism as a relatively avirulent opportunistic pathogen. we present an unusual case of rapidly fatal necrotizing fasciitis and septic shock due to myroides odoratus. our case demonstrates the pathogenicity of myroides, and highlights potential risk factors for infection including ...201424806817
phylum-wide general protein o-glycosylation system of the bacteroidetes.the human gut symbiont bacteroides fragilis has a general protein o-glycosylation system in which numerous extracytoplasmic proteins are glycosylated at a three amino acid motif. in b. fragilis, protein glycosylation is a fundamental and essential property as mutants with protein glycosylation defects have impaired growth and are unable to competitively colonize the mammalian intestine. in this study, we analysed the phenotype of b. fragilis mutants with defective protein glycosylation and found ...201323551589
dna-dna hybridization study of strains of chryseobacterium, elizabethkingia and empedobacter and of other usually indole-producing non-fermenters of cdc groups iic, iie, iih and iii, mostly from human clinical sources, and proposals of chryseobacterium bernardetii sp. nov., chryseobacterium carnis sp. nov., chryseobacterium lactis sp. nov., chryseobacterium nakagawai sp. nov. and chryseobacterium taklimakanense comb. nov.the taxonomic classification of 182 phenotypically similar isolates was evaluated using dna-dna hybridization and 16s rrna gene sequence analysis. these bacterial isolates were mainly derived from clinical sources; all were gram-negative non-fermenters and most were indole-producing. phenotypically, they resembled species from the genera chryseobacterium, elizabethkingia or empedobacter or belonged to cdc groups iic, iie, iih and iii. based on these analyses, four novel species are described: ch ...201323934253
myroides indicus sp. nov., isolated from garden soil.a novel aerobic, non-motile, rod-shaped, catalase- and oxidase-positive bacterial strain, designated uks3t, was isolated from garden soil, and subjected to polyphasic taxonomic analysis. strain uks3t formed whitish, viscous colonies on nutrient agar and was gram-staining negative. phylogenetic analysis, based on 16s rrna gene sequence, showed that maximum pairwise similarity occurs with representatives of the genus myroides. the most closely related species include myroides marinus js-08t (92.7  ...201526276159
comparison of vitek2, maldi-tof ms, and 16s rdna sequencing for identification of myroides odoratus and myroides odoratimimus.the genus myroides comprises the 2 medically relevant species myroides odoratus and myroides odoratimimus that are rare opportunistic pathogens and cause infections in immunocompromised patients. a fast identification of myroides is of importance because these bacterial strains show multiple resistance against antibiotics and therefore limit treatment options. they are associated, for instance, with urinary tract infections, sepsis, meningitis, pneumonia, and infectious cellulitis. since more an ...201424666701
myroides odoratus and chryseobacterium indologenes: two rare isolates in the immunocompromised.myroides spp and chryseobacterium spp are uncommon clinical isolates, though more frequently reported to cause infections than other pigmented non-fermentors. two cases of myroides odoratus and chryseobacterium indologenes infection in a diabetic with pulmonary tuberculosis and a patient with de-compensated alcoholic liver disease, respectively, are reported here. anti-microbial susceptibility testing of the isolates was performed by determining the minimum inhibitory concentration. the clinical ...201625008832
septic shock due to myroides odoratus in a medical intensive care unit patient with severe necrotising pancreatitis. 201627029668
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