| serological assessment of helicobacter pylori eradication. | | 1992 | 1359233 |
| isolation of helicobacter pylori from human faeces. | helicobacter pylori is arguably the commonest chronic infection in man. however, its route of transmission is unknown. we have isolated viable h pylori from the faeces of an infected individual from the gambia. the organism was cultured on selective media after concentration of faecal bacteria by centrifugation in a buffer equilibrated with a microaerophilic gas mixture. growth characteristics, microscopic appearances, and enzyme activities were the same as those of a typical gastric isolate of ... | 1992 | 1359263 |
| [drug therapy of peptic ulcer in the elderly]. | medical therapy of peptic ulcers in elderly patients should depend on drugs which have to be administered only once daily. in addition, the ideal compound should have minimal side effects as well as minimal, if any, interactions with concomitant forms of therapy. from this point of view, acid-inhibitory drugs are preferable to other anti-ulcer drugs, the latter having little importance in advanced age. for ulcer healing, the proton pump inhibitor omeprazole is recommended, whereas h2-blockers (p ... | 1992 | 1359717 |
| studies on antiulcer drugs. vi. 4-furyl-2-guanidinothiazoles and related compounds as potent histamine h2-receptor antagonists. | a series of 4-furyl-2-guanidinothiazole derivatives and related compounds were synthesized and evaluated for histamine h2-receptor antagonist and gastric acid antisecretory activities. among them, compounds i-17, i-48 and i-49 showed high activities in these tests. in addition, compound i-17 possessed potent inhibitory activities on each of the gastric ulcers induced by stress, ethanol and hcl-aspirin. on the other hand, compound i-48 demonstrated antimicrobial activity against helicobacter pylo ... | 1992 | 1359931 |
| helicobacter pylori and peptic ulcers in rheumatoid arthritis patients receiving gold, sulfasalazine, and nonsteroidal anti-inflammatory drugs. | the conflicting reports on gold and helicobacter pylori could be related to the use of serological tests of unproven value in nsaid patients, and to the lack of the appropriate control groups. more important is the fact that the endoscopic consequences of the possible effect of gold on h. pylori have not been investigated. we therefore decided to assess the prevalence of h. pylori and peptic ulcers in rheumatoid patients being treated with gold sodium thiomalate (gst) plus nsaid, sulfasalazine p ... | 1992 | 1360191 |
| pcr-based rflp analysis of dna sequence diversity in the gastric pathogen helicobacter pylori. | dna sequence diversity among 60 independent isolates of the gastric pathogen helicobacter pylori was assessed by testing for restriction fragment length polymorphisms (rflps) in several pcr-amplified gene segments. 18 mbol and 27 haeiii rflps were found in the 2.4 kb urea-ureb (urease) segment from the 60 strains; this identified 44 separate groups, with each group containing one to four isolates. with one exception, each isolate not distinguished from the others by rflps in urea-ureb was distin ... | 1992 | 1361982 |
| bacterial flagellar diversity and significance in pathogenesis. | bacterial flagella are structurally diverse, ranging from the thoroughly investigated model examples found in escherichia coli and salmonella typhimurium to the more exotic sheathed flagella of, for example, helicobacter pylori, and the complex multi-flagellin endoflagella found in many spirochaetes. we summarize some of the emerging structural and genetic findings relating to these more novel flagellar types, and outline their possible significance in the pathogenicity of some medically importa ... | 1992 | 1362173 |
| [current alteration of helicobacter pylori in gastro-duodenal diseases]. | the current alteration of the detection rate of helicobacter pylori (h. pylori) on mucous membranes of the stomach has been surveyed and reviewed for 49 cases of gastro-duodenal diseases. the survey has been conducted for 3 years and an endoscopic examinations have been performed 2-8 times. on the first visit, the diagnosis can be made endoscopically, and h2 blockers and another agents were used as therapeutic drugs. the detection rate was 67.3% (33 cases) for positive cases and positive reactio ... | 1992 | 1363434 |
| resistance to medical ulcer therapy--possible reasons and management. | in 112 patients with duodenal ulcer (males, mean age 29, range 18-35) with a history of at least 3 years, and never being treated with h2-antagonists, maintenance therapy with tagamet was started. all of them had ulcer recurrence when entering the study. the first two months a full dose was applied, after this bedtime doses up to 24 months. endoscopy was performed at the beginning, after the first and second months and then every two-month period. gastric secretion was measured every 2-3 months. ... | 1992 | 1364278 |
| [does helicobacter pylori cause stomach cancer?]. | | 1991 | 1365939 |
| helicobacter mustelae isolation from feces of ferrets: evidence to support fecal-oral transmission of a gastric helicobacter. | helicobacter mustelae has been isolated from stomachs of ferrets with chronic gastritis and ulcers. when h. mustelae is inoculated orally into h. mustelae-negative ferrets, the animals become colonized and develop gastritis, a significant immune response, and a transient hypochlorhydria. all of these features mimic helicobacter pylori-induced gastric disease in humans. because the epidemiology of h. pylori infection is poorly understood and its route of transmission is unknown, the feces of wean ... | 1992 | 1370432 |
| rapid detection of helicobacter pylori in gastric biopsy material by polymerase chain reaction. | by using primers based on the sequence of a species-specific antigen of helicobacter pylori (p. o'toole, s.m. logan, m. kostrzynska. t. wadström, and t.j. trust, j. bacteriol. 173:505-513, 1991), a protocol was established for detection of this microorganism in gastric biopsy samples by the polymerase chain reaction (pcr). a single primer pair was used to specifically amplify a 298-bp sequence in a rapid two-step pcr. the primers exhibited the same specificity in pcr as that which we reported fo ... | 1992 | 1370850 |
| gastric histamine concentration and ige in helicobacter pylori infection. | | 1992 | 1371778 |
| ribosomal rna gene patterns of helicobacter pylori from surgical patients with healed and recurrent peptic ulcers. | fifty-two strains of helicobacter pylori were examined by dna restriction endonuclease digestion, ribosomal (r)rna gene probe hybridization and biotyping. most (49) strains originated from gastric (antral) biopsies of patients before or after elective surgery for duodenal ulcers. chromosomal dna hind iii ribopatterns showed 9 strain clusters of which the largest contained 12 strains each with 3 common bands (1.50, 3.45, and 4.26 kb) but which were heterogeneous with respect to biotype and total ... | 1992 | 1372263 |
| glycosulfatase activity of helicobacter pylori toward gastric mucin. | a glycosulfatase activity toward sulfated gastric mucus glycoprotein was identified in the extracellular material elaborated by h. pylori, a bacteria implicated in the etiology of gastric disease. upon acetone precipitation, an active enzyme fraction at 64% acetone was obtained which on sds-page gave a major 30kda protein band. the h. pylori glycosulfatase exhibited maximum activity (314.8 pmol/mg protein/h) at ph 5.7 in the presence of triton x-100 and cacl2, and was capable of removal of the s ... | 1992 | 1372502 |
| use of romanowsky type (diff-3) stain for detecting helicobacter pylori in smears and tissue sections. | a romanowsky type (diff-3) stain was used for identifying helicobacter pylori in gastric biopsy specimens from 50 patients with ulcer and non-ulcer dyspepsia. air dried smears were prepared from fresh biopsy tissue and histological sections were prepared from paraffin wax processed tissue. the diff-3 technique is accomplished in five steps and takes about 30 seconds. results using the diff-3 stain correlated 100% with those using the giemsa stain. the diff-3 stain is reliable, simple, rapid, eas ... | 1992 | 1375949 |
| effect of helicobacter pylori infection on colloidal bismuth subcitrate concentration in gastric mucus. | necropsy gastric mucus infected with helicobacter pylori has a reduced capacity to concentrate colloidal bismuth subcitrate when compared with non-infected mucus. mucus mounted in a modified in vitro diffusion chamber was bathed with colloidal bismuth subcitrate solutions at different concentrations and ph levels. bismuth was measured by atomic absorption spectrophotometry to assess intramucus colloidal bismuth subcitrate concentrations. bismuth concentrations in non-infected mucus were higher t ... | 1992 | 1377152 |
| ribotyping of helicobacter pylori from clinical specimens. | ribotyping is a method used to type strains of bacteria by analyzing the restriction enzyme digestion patterns of the rrna genes. this method was applied to 126 strains of helicobacter pylori from 100 unrelated symptomatic patients who had endoscopies done and to 15 strains from 15 infected subjects from seven families. analysis of the rrna gene patterns revealed 77 distinct ribotypes from the 100 patients. from 15 of these subjects, isolates were recovered from antral mucosal biopsies at follow ... | 1992 | 1378063 |
| mechanism of helicobacter pylori pathogenesis: focus on mucus. | although the clinical data provide increasingly convincing indications that helicobacter pylori (h. pylori) is a causative factor in gastritis and peptic ulcer, the advances toward the clear understanding of this bacterium's pathogenic action are slow in coming. having a niche bordering two major perimeters of gastric mucosal defense, h. pylori is capable of exerting detrimental effects on the mucus layer, as well as surface cells of the gastric epithelium. to cause such an effect, the bacteria ... | 1992 | 1378466 |
| clarithromycin. a review of its antimicrobial activity, pharmacokinetic properties and therapeutic potential. | clarithromycin is an acid-stable orally administered macrolide antimicrobial drug, structurally related to erythromycin. it has a broad spectrum of antimicrobial activity, similar to that of erythromycin and inhibits a range of gram-positive and gram-negative organisms, atypical pathogens and some anaerobes. significantly, clarithromycin demonstrates greater in vitro activity than erythromycin against certain pathogens including bacteroides melaninogenicus, chlamydia pneumoniae, chlamydia tracho ... | 1992 | 1379907 |
| ribosomal rna gene restriction patterns of helicobacter pylori: analysis and appraisal of hae iii digests as a molecular typing system. | ribosomal rna gene restriction patterns (ribopatterns) of 162 strains of helicobacter pylori from 93 patients were studied to assess their suitability for use as the basis of a molecular typing system. computer-assisted numerical analysis of hae iii ribopatterns of 122 strains from 9 countries in 4 continents showed that almost every strain had a distinct and unique ribopattern and only strains from the same individual were genomically matched in all bands or with minor (1-2 band) differences. h ... | 1992 | 1379934 |
| characterization of hela cell vacuoles induced by helicobacter pylori broth culture supernatant. | helicobacter pylori broth culture supernatants induce eukaryotic cell vacuolation in vitro, a phenomenon that has been attributed to cytotoxic activity. we sought to characterize further the vacuolation of hela cells that occurs in response to h pylori culture supernatant. nascent vacuoles were detectable by electron microscopy after 90 minutes of incubation with h pylori supernatant and were not associated with any identifiable organelle. after 6 days of incubation with h pylori supernatant, va ... | 1992 | 1381332 |
| glycosulfatase activity of h. pylori toward human gastric mucin: effect of sucralfate. | colonization of gastric mucosa by helicobacter pylori, a bacterium implicated in the etiology of gastric disease, involves the cell surface sulfated glycosphingolipid receptors for the attachment. evidence has also been obtained recently that sulfated mucus glycoproteins have the ability to interfere with this process. here, we show that h. pylori displays glycosulfatase activity, and report the specificity of this enzyme toward gastric mucosal sulfated glycoproteins and glycolipids. with 35s-la ... | 1992 | 1381553 |
| monoclonal antibodies against the native urease of helicobacter pylori: synergistic inhibition of urease activity by monoclonal antibody combinations. | monoclonal antibodies (mabs) against the native urease of helicobacter pylori nctc 11637 were found to clearly inhibit the urease activity. interestingly, synergistic inhibition by two mabs recognizing different subunits was also observed. ten mabs were produced and classified as two isotypes of the immunoglobulin g (igg) subclass, igg1, and igg2a. western blot (immunoblot) analysis using sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that five mabs recognized the large subunit ... | 1992 | 1383158 |
| reverse transcription and polymerase chain reaction amplification of rrna for detection of helicobacter species. | sequence data on helicobacter pylori 16s rrna were used to select two 22-base oligonucleotide primers for use in a polymerase chain reaction (pcr) for detection of h. pylori. h. pylori cells were treated with lysis buffer, boiled, and chloroform extracted. reverse transcription of rrna was followed by pcr amplification (rt-pcr) of the synthesized cdna and 16s rrna gene. the amplified pcr products were analyzed by agarose gel electrophoresis and southern blotting. using ethidium bromide-stained a ... | 1992 | 1383268 |
| vitronectin binding by helicobacter pylori. | vitronectin, a serum and extracellular matrix protein involved in immunological reactions, interacts with helicobacter pylori strains. of the 20 h. pylori strains tested three strains bound more than 50% of the vitronectin added, five strains bound 25-40%, nine strains bound 10-20% and three strains bound 5-8% vitronectin. two strains, one with high- and one with low-binding properties, were selected for further characterization of 125i-vitronectin binding. binding to the urea-activated 125i-lab ... | 1992 | 1384604 |
| inhibition of gastric mucosal laminin receptor by helicobacter pylori lipopolysaccharide: effect of ebrotidine. | the effect of lipopolysaccharide from h. pylori, a bacteria implicated in the etiology of gastric disease, on the gastric mucosal laminin-receptor interaction was investigated. the receptor protein, prepared from rat gastric epithelial cell membrane by affinity chromatography on laminin-coupled sepharose, was radioiodinated, and incorporated into liposomes which exhibited specific affinity towards laminin-coated surface. the binding was inhibited by h. pylori lipopolysaccharide, which caused a m ... | 1992 | 1385705 |
| refractory peptic ulcers. | ulcers that do not heal after 8 weeks of treatment with standard-dose regimens of antiulcer drugs are considered refractory. incidences of duodenal and gastric ulcers refractory to h2-receptor antagonists are 9% and 20%, respectively. when treated with a single daily dose of omeprazole 20 mg, duodenal ulcers have a refractory incidence of 3% and gastric ulcers, 11%. omeprazole's benefit may result from its potent gastric antisecretory action. acid hypersecretion is an important pathophysiologic ... | 1992 | 1386767 |
| prevalence of antibodies against heat-stable antigens from helicobacter pylori in patients with dyspeptic symptoms and normal persons. | heat-stable antigens from helicobacter pylori were investigated for the detection of serum igg, iga and igm antibodies against h. pylori by an elisa technique. antibody titers against h. pylori were measured in 167 dyspeptic patients, of whom 96 were h. pylori positive confirmed by culture or microscopy, and in 482 controls (0-98 years). increased igg antibody titers were found significantly more often in dyspeptic patients with active chronic gastritis than in patients with normal morphology, a ... | 1992 | 1389098 |
| [helicobacter pylori]. | | 1992 | 1391020 |
| treatment of peptic ulcer disease with furazolidone. | furazolidone (fz) has been used in china as a treatment of peptic ulcer disease for about 20 years. clinical and experimental studies suggest that it has good short-term and long-term effects on both human and animal ulcers. the ulcer healing rate is related to the dosage and course of treatment. the healing rate of a high dose, 2 week course is about 70-75% and the relapse rate after 3 years is 9.5%. the adverse reactions to fz are not severe, and are well tolerated in most patients. however th ... | 1992 | 1391736 |
| preliminary observations in the fasting serum gastrin in patients with duodenal ulcer; further evidence of the "clearing" effect of omeprazole on h pylori? | thirty patients with active duodenal ulcer who were helicobacter pylori positive (hp+) by hlo test and by histology (giemsa stain) were given omeprazole (ome) 20 mg/d for a two-week period. estimation of fasting serum gastrin concentration (ria) was performed before treatment and 24 hours after the last dosage of ome, and hp was searched for an antral biopsies at the end of the treatment as well. mean fasting serum gastrin concentration increased significantly after treatment in all patients stu ... | 1992 | 1392017 |
| the association of helicobacter pylori infection with low levels of urea and ph in the gastric juices. | fifty-four gastric biopsies and their relative gastric juices were analyzed for the presence of helicobacter pylori with both cultural and microscopic methods. thirty-one samples were positive and twenty-three were negative. these data were therefore employed as references for the subsequent comparisons. furthermore, the gastric juices were later tested to establish the urea concentration and the ph level. in addition, the sediment obtained after centrifugation was microscopically observed for t ... | 1992 | 1392020 |
| double blind trial of colloidal bismuth subcitrate versus placebo in helicobacter pylori positive patients with non-ulcer dyspepsia. | we have carried out a double blind placebo controlled trial to assess the effects of treatment with colloidal bismuth subcitrate in helicobacter pylori associated non-ulcer dyspepsia. eighty patients with dyspepsia, normal upper gastrointestinal appearances at endoscopy and h pylori associated active chronic gastritis on histology of gastric antral biopsies were included in the trial. the patients were randomised to receive colloidal bismuth subcitrate 240mg twice daily for four weeks or matchin ... | 1992 | 1392023 |
| helicobacter pylori: a nihilistic perspective. | | 1992 | 1392405 |
| maintenance therapy for peptic ulcer disease: an overview of medical treatment. | peptic ulcer disease is a chronic disorder that manifests as recurrent episodes of pain or complications such as bleeding or perforation. current medical therapy with h2-receptor blockers or sucralfate effectively prevents most recurrences and, according to clinical trials, may prevent complications. since this treatment does not alter the natural history of peptic ulcer disease, continued therapy is needed. surgical treatment does permanently alter the history of recurrence. medical therapy aim ... | 1992 | 1392406 |
| randomised controlled trial of short term treatment to eradicate helicobacter pylori in patients with duodenal ulcer. | to determine whether one week's drug treatment is sufficient to eradicate helicobacter pylori in patients with duodenal ulcer. | 1992 | 1392995 |
| gas phase derivatization of ammonia with 4-fluoro-7-nitrobenzo-2-oxa-1,3-diazole and its application to urease assay. | an ammonia-specific and rapid fluorometric method for determination of ammonia and urease activity was developed. the method is designed to assay ammonia levels or urease activity for the rapid diagnosis of helicobacter pylori infection. 4-fluoro-7-nitrobenzo-2-oxa-1,3-diazole was used to derivatize ammonia and 4-amino-7-nitrobenzo-2-oxa-1,3-diazole was analysed by high performance liquid chromatography at an excitation wavelength of 455 nm and an emission wavelength of 520 nm. derivatization wa ... | 1992 | 1394655 |
| [the advances in immunological research of helicobacter pylori]. | | 1992 | 1395917 |
| significance and regulation of gastric secretion of platelet-activating factor (paf-acether) in man. | platelet-activating factor (paf) has been implicated in the pathogenesis of acute inflammatory and ulcerative diseases of the upper gastrointestinal tract. in the present study, we compared the gastric output of paf and its precursors with gastric acid output, in patients with various upper gastrointestinal tract diseases and healthy controls. paf and precursors were also extracted from gastric biopsies from subjects with chronic gastritis and/or gastric colonization by helicobacter pylori. unde ... | 1992 | 1396006 |
| use of a urea breath test versus invasive methods to determine the prevalence of helicobacter pylori in zaire. | the prevalence of helicobacter pylori infection in zaire was determined by means of a [14c] urea breath test in 133 asymptomatic subjects, by culture and histological examination of biopsies in 324 consecutive endoscopy patients with chronic epigastric complaints, and by both the breath test and culture/histology in a subset of 92 patients. sixty healthy belgian students or hospital laboratory workers were also included for comparison. the prevalence of helicobacter pylori was significantly high ... | 1992 | 1396751 |
| an assessment of serological tests for detection of helicobacter pylori. | | 1992 | 1396763 |
| effect of antimicrobial therapy on the specific serological response to helicobacter pylori infection. | the systemic immune response to helicobacter pylori was studied in 247 infected adult patients before antimicrobial therapy and at different intervals following therapy. endoscopy with simultaneous collection of biopsies was performed in all patients immediately before treatment, 4 to 6 weeks after the end of therapy and 6 to 12 months later. a 14c-urea breath test was performed 3 to 6 months after the end of treatment. biopsy specimens were cultured and examined histologically using giemsa stai ... | 1992 | 1396764 |
| serodiagnosis of helicobacter pylori infections by detection of immunoglobulin g antibodies using an immunoblot technique and enzyme immunoassay. | a transferable solid phase enzyme immunoassay (tsp-eia) and an immunoblot technique were evaluated for the detection of igg antibodies against helicobacter pylori. using the biopsy urease test as reference method, the sensitivity and specificity of the eia were 96% and 100%, respectively. immunoblot analysis was carried out by testing sera from patients with a positive urease test who suffered from type b gastritis, gastric and duodenal ulcers, and a negative control group. the immunoblotted hel ... | 1992 | 1396765 |
| serodiagnosis of helicobacter pylori infections with an enzyme immunoassay using the chromatographically purified 120 kilodalton protein. | a membrane-associated 120 kda protein of helicobacter pylori with known species-specificity was isolated and used in an enzyme immunoassay (eia) for the detection of helicobacter pylori-specific igg antibodies in patient sera. the eia was compared with two other methods used for serodiagnosis of helicobacter pylori infections: an eia using sonicated whole helicobacter pylori cell antigen and western immunoblot. in a prospective study 127 unselected patients (76 patients with antrum gastritis, 51 ... | 1992 | 1396766 |
| evaluation of two commercial enzyme immunoassays for the diagnosis of helicobacter pylori infection. | sera from 65 patients with upper gastrointestinal tract symptoms and proven helicobacter pylori infection, and from 42 negative controls were tested with two commercial eias (gap test, biorad; and ecp test, biometra) and two non-commercial eias, one performed with whole sonicated cells and the other with acid extract of helicobacter pylori as antigen. the gap assay showed a sensitivity of 83.1% and a specificity of 47.6%. the ecp assay showed a sensitivity of 87.7% and a specificity of 61.9%. fo ... | 1992 | 1396775 |
| helicobacter pylori, peptic ulcer disease and inhibition of gastric acid secretion. | recent studies have been reviewed to establish the possible importance of the interaction between helicobacter pylori infection and gastric acid secretion. h. pylori infection results in increased gastrin release, but this does not lead to gastric acid hypersecretion and gastrin normalizes after eradication of the infection. an optimal, well-tolerated treatment strategy against h. pylori infection has not yet been clearly defined. one potentially useful approach may be to improve the antibacteri ... | 1992 | 1397740 |
| natural history of gastritis and its relationship to peptic ulcer disease. | chronic gastritis is a common inflammatory disease. in a number of patients, the inflamed gastric mucosa shows a gradual tendency to become atrophic (atrophic gastritis). gastritis tends to be lifelong, and spontaneous healing is rare. with very few exceptions (e.g. in patients with autoimmune chronic corpus gastritis), gastritis is associated with the presence of the bacterium helicobacter pylori. inflammation and atrophy of the gastric mucosa result in impairment of gastric secretory functions ... | 1992 | 1397747 |
| helicobacter pylori secretes a chemotactic factor for monocytes and neutrophils. | helicobacter pylori is associated with an inflammatory reaction in the stomach and duodenum, yet the mechanism of this inflammatory infiltrate is unknown. the ability of helicobacter pylori to secrete a factor that attracts leucocytes is investigated. helicobacter pylori conditioned supernatant attracted neutrophils and monocytes with 50-100% of the activity of control chemotactic factor, 10(-8) m formyl-methionine-leucine-phenylalanine. strains derived from individuals with ulcer or non-ulcer a ... | 1992 | 1398224 |
| effect of helicobacter pylori infection on 24 hour intragastric acidity in patients with gastritis and duodenal ulcer. | helicobacter pylori status, gastric histology, and 24 hour acidity were studied in 35 gastritis patients, 21 duodenal ulcer patients, and 14 subjects with normal gastric mucosa. h pylori was identified in 21 of 35 patients with chronic active gastritis and in 19 of 21 duodenal ulcer patients, but in none of those with normal gastric mucosa. mean scores of activity of gastritis were similar in h pylori positive gastritis and duodenal ulcer patients, but were significantly lower in h pylori negati ... | 1992 | 1398225 |
| helicobacter pylori and gastric cancer: correlation with gastritis, intestinal metaplasia, and tumour histology. | this study aimed to examine the association between helicobacter pylori, histological gastritis, and intestinal metaplasia in gastric cancers of different histological types. a total of 169 gastrectomy specimens received in one pathology department were studied. altogether 156 were adenocarcinomas (intestinal type 87, diffuse type 50, mixed type 19). gastritis occurred in 137 of 163 body specimens (84%) and in 126 of 131 antral specimens (96%). its presence was unrelated to tumour histology. atr ... | 1992 | 1398226 |
| [chronic gastritis--does it need treatment and is it curable?]. | fundamental considerations: addition to our medical knowledge of chronic gastritis and its sequelae; changes in nomenclature. methods/content: presentation of the pathophysiological situation (processes) oft colonization of the gastric mucosa by helicobacter pylori with particular consideration being given to histomorphological findings. discussion of the relative risk of gastritis patients contracting a gastric or duodenal ulcer, or possibly developing carcinoma of the stomach. conclusions: on ... | 1992 | 1398409 |
| lipid a in helicobacter pylori. | free lipid a of helicobacter pylori was characterized with regard to chemical composition, reactivity with anti-lipid a antibodies, and activity in a limulus lysate assay. the predominant fatty acids of h. pylori lipid a were 3-oh-18:0, 18:0, 3-oh-16:0, 16:0, and 14:0. hexosamine was present in amounts similar to those in campylobacter jejuni or salmonella typhimurium lipid a. the lipopolysaccharide of h. pylori contained 2-keto-3-deoxyoctonic acid, a common constituent of enterobacterial and c. ... | 1992 | 1398948 |
| activity of the antimycotic ketoconazole against helicobacter pylori. | | 1992 | 1399937 |
| construction of a helicobacter pylori genome map and demonstration of diversity at the genome level. | genomic dna from 30 strains of helicobacter pylori was subjected to pulsed-field gel electrophoresis (pfge) after digestion with noti and nrui. the genome sizes of the strains ranged from 1.6 to 1.73 mb, with an average size of 1.67 mb. by using noti and nrui, a circular map of h. pylori ua802 (1.7 mb) which contained three copies of 16s and 23s rrna genes was constructed. an unusual feature of the h. pylori genome was the separate location of at least two copies of 16s and 23s rrna genes. almos ... | 1992 | 1400229 |
| specific detection of campylobacter jejuni and campylobacter coli by using polymerase chain reaction. | development of a routine detection assay for campylobacter jejuni and campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction (pcr). an oligonucleotide primer pair from a conserved 5' region of the flaa gene of c. coli vc167 was used to amplify a 450-bp region by pcr. the primer pair specifically detected 4 strains of c. coli and 47 strains of c. jejuni; but it did not detect strains of campylobacter fetus, campylobacter lari, campylobacter upsaliensis, cam ... | 1992 | 1400961 |
| serological detection of helicobacter pylori by a flow microsphere immunofluorescence assay. | a flow cytometric immunofluorescence assay (fmia) for the detection of immunoglobulin g antibodies to helicobacter pylori was developed. a multicomponent antigen was prepared and used to coat carboxylated polystyrene microspheres for reaction with patient sera followed by fluorescein isothiocyanate-labelled goat anti-human immunoglobulin g. the reacted microspheres were collected with a flow cytometer, and fluorescence was quantitated relative to the cutoff value provided by pooled sera from pat ... | 1992 | 1400995 |
| diagnostic tests for helicobacter pylori: comparison and influence of non-steroidal anti-inflammatory drugs. | to evaluate the efficacy of culture, histology, clo-test, helico-g and pyloriset tests in diagnosing helicobacter pylori in the presence or absence of non-steroidal anti-inflammatory drugs (nsaids). | 1992 | 1401184 |
| expression of 120 kilodalton protein and cytotoxicity in helicobacter pylori. | antral biopsy culture supernatants from 14 subjects with chronic gastritis, known to have iga antibodies to the 120 kilodalton protein, showed positive recognition of this antigen in western blots against a cytotoxin positive strain of helicobacter pylori but gave negative reactions with two cytotoxin negative strains. control immunoblots with culture supernatants from 13 non-responders to the protein were all negative. this indicates a direct association between expression of the 120 kilodalton ... | 1992 | 1401190 |
| selected cryopreservatives for long term storage of helicobacter pylori at low temperatures. | to meet the need for information on cryopreservation, a study was done on 32 helicobacter pylori strains, comparing different cryopreservative media. sheep blood, horse blood, horse serum with and without glycerol, and mineral oil media were used for long term storage of h pylori at -70 degrees c or in liquid nitrogen. procedures were developed which permitted recovery of 87.5% of the strains included in the study after they had been stored for 24 months. of those strains stored for more than th ... | 1992 | 1401191 |
| successful freeze storage and lyophilisation for preservation of helicobacter pylori. | long term storage techniques for the preservation of helicobacter pylori were developed. the cells survived at -75 degrees c in the presence of glycerol and at +4 degrees c after freeze-drying. both techniques are suitable for routine use. | 1992 | 1401192 |
| quantitative assessment of histological changes in chronic gastritis after eradication of helicobacter pylori. | to evaluate the effect of 10 day triple treatment on h pylori eradication and associated gastritis. | 1992 | 1401210 |
| potentiation of helicobacter pylori vacuolating toxin activity by nicotine and other weak bases. | about 50% of helicobacter pylori isolates produce a vacuolating toxin in vitro, which may be an important determinant of virulence. because ammonium salts potentiate h. pylori toxin activity, the effect of other weak bases upon toxin activity was determined. vacuolation of hela cells was quantitated using a neutral red uptake assay. as expected, ammonium chloride, trimethylamine, triethanolamine, and nicotine each induced vacuolation of hela cells when tested independently. in addition, each of ... | 1992 | 1402018 |
| [proton pump blockade and helicobacter pylori: status of current knowledge and clinical importance]. | | 1992 | 1406011 |
| alcohol dehydrogenase mediated acetaldehyde production by helicobacter pylori--a possible mechanism behind gastric injury. | two standard helicobacter pylori strains showed significant cytosolic alcohol dehydrogenase activity and produced considerable amounts of acetaldehyde when incubated with an ethanol containing solution in vitro. the alcohol dehydrogenase activity of the helicobacter pylori strains was almost as high as that found in klebsiella pneumoniae and far greater than that in escherichia coli or campylobacter jejuni. the amount of acetaldehyde produced by cytosol prepared from helicobacter pylori exceeded ... | 1992 | 1406052 |
| contribution of the 13c-urea breath test to the detection of helicobacter pylori gastritis in children. | serology, 13c-urea breath test, histology, campylobacter-like organism testing, and culture were performed in 95 consecutive children to evaluate the contribution of these tests to the detection of helicobacter pylori infection. in analyses considering any combination of three positive tests as "gold standard" for diagnosing h pylori infection, 26 children were helicobacter positive (27%), which is only one patient more than the number of children with only a positive culture. the accuracy of cu ... | 1992 | 1408517 |
| dna diversity among clinical isolates of helicobacter pylori detected by pcr-based rapd fingerprinting. | the rapd (or ap-pcr) dna fingerprinting method was used to distinguish among clinical isolates of helicobacter pylori, a bacterium whose long term carriage is associated with gastritis, peptic ulcers and gastric carcinomas. this method uses arbitrarily chosen oligonucleotides to prime dna synthesis from genomic sites to which they are fortuitously matched, or almost matched. most 10-nt primers with > or = 60% g + c yielded strain-specific arrays of up to 15 prominent fragments, as did most longe ... | 1992 | 1408828 |
| triple therapy with sucralfate is as effective as triple therapy containing bismuth in eradicating helicobacter pylori and reducing duodenal ulcer relapse rates. | duodenal ulcer relapse rates after therapy with sucralfate or bismuth are lower than those after h2-receptor antagonist therapy. this may be mediated by an antibacterial effect of these drugs on helicobacter pylori. bismuth has become an integral part of 'triple therapy' because of its documented anti-h. pylori effect. in vitro and clinical data suggest that sucralfate may also have an anti-h. pylori effect. the aim of this randomized, prospective therapeutic trial was to compare the efficacy of ... | 1992 | 1411294 |
| cell surface proteins of helicobacter pylori as antigens in an elisa and a comparison with three commercial elisa. | cell surface proteins of helicobacter pylori were solubilized by extraction with acidic glycine buffer, n-octyl-glucoside, lithium chloride, and distilled water, and by sonication. the preparations were evaluated as antigens in elisa to detect serum igg responses in patients and healthy subjects. sds-page analyses of the preparations from a type strain (nctc 11637) and of acidic glycine extracts of 4 clinical isolates showed multiple protein bands. the sera were classified as hp+ve and hp-ve by ... | 1992 | 1411312 |
| [comparison of the efficacy of omeprazole/bismuth subcitrate or triple therapy in helicobacter pylori gastritis. a prospective controlled study]. | in a controlled, prospective, randomized trial, 10 patients with helicobacter pylori positive gastritis were treated either with triple therapy (tetracycline, ornidazole, bismuth subcitrate; t, or, cbs), or omeprazole/cbs (o/cbs) to test the eradication rate of each treatment, its effect on gastritis and meal stimulated gastrin release. | 1992 | 1411403 |
| clinical value of endoscopy and histology in the diagnosis of duodenogastric reflux disease. | the endoscopic observation of a bile lake in the stomach, antral gastritis, or ulcerations and the histologic finding of foveolar hyperplasia or chronic gastritis have been implicated as indicators of excessive duodenogastric reflux. the accuracy of these criteria was evaluated in 135 patients with nonspecific symptoms in the foregut suggestive of duodenogastric reflux and no evidence for alcohol- or drug-induced gastric mucosal injury. | 1992 | 1411953 |
| [helicobacter pylori and gastrointestinal tract diseases]. | we review the bacteriological, epidemiological and pathogenetic aspects of helicobacter pylori and related diseases of the gastrointestinal tract. it is concluded that h. pylori seems to be at least one of several etiological factors for diseases such as chronic gastritis and duodenal ulcer. it may also be an important cofactor in the development of gastric carcinoma. | 1992 | 1412235 |
| [diagnosis of helicobacter pylori infection. rapid urease test, microscopy of smears and culture from ventricular biopsy compared with the 14c-urea breath test]. | by employing the 14c-urea breath test as the reference method we determined the specificity and sensitivity of three bioptic methods for diagnosis of helicobacter pylori infection in 103 subjects. all biopsy specimens were obtained from the gastric antrum. for culture the specificity was 100%. its applicability was reduced, however, by a low sensitivity (73.8%) and a delay of several days before the final result was available. microscopy of loeffler-stained biopsy smears yielded a specificity of ... | 1992 | 1412236 |
| [detection of helicobacter pylori infections by antibody determination]. | | 1992 | 1413904 |
| helicobacter pylori and recurrence of duodenal ulcers. | | 1992 | 1415088 |
| smoking does not contribute to duodenal ulcer relapse after helicobacter pylori eradication. | cigarette smoking is believed to be one of the major factors influencing duodenal ulcer (du) recurrence. however, the influence of cigarette smoking on du recurrence after the eradication of helicobacter pylori has not been separately addressed. the aim of this study was to investigate du relapse rate in smokers and nonsmokers, both with confirmed eradication of h. pylori. patients with h. pylori eradication, demonstrated at endoscopy 4 wk post-treatment, were included in the study. smoking hist ... | 1992 | 1415092 |
| nonsteroidal antiinflammatory drug-associated gastric ulcers do not require helicobacter pylori for their development. | nonsteroidal antiinflammatory drugs (nsaids) have been linked with a high incidence of ulcer complications. histologic gastritis is present in most patients with standard peptic ulcers, and this gastritis is generally related to helicobacter pylori (hp). we questioned whether gastric ulcers associated with nonsteroidal antiinflammatory drugs (nsaids) develop via a novel mechanism, distinct from the usual hp-gastritis-ulcer diathesis. two groups of patients with newly discovered gastric ulcers we ... | 1992 | 1415094 |
| helicobacter pylori-negative gastric ulcer. | causes of helicobacter pylori (hp)-negative gastric ulcers (gus) have not previously been systematically studied. in this study we examined possible causes of hp-negative gus. among 115 consecutive patients with endoscopic diagnoses of gu, 71 (62%) had hp infection, and 44 (38%) were found to be hp-negative. of the 71 hp-positive patients, 47 (66%) had no other detectable causal factors, 21 (30%) were regularly taking nonsteroidal antiinflammatory drugs (nsaids), and three (4%) had malignant gu. ... | 1992 | 1415095 |
| bleeding from a meckel's diverticulum after the use of ibuprofen. | a meckel's diverticulum is an unusual site of gastrointestinal bleeding in adults. bleeding often results from ulceration of ileal mucosa that lies adjacent to ectopic gastric mucosa. we report on a 27-yr-old man who bled from a meckel's diverticulum after receiving oral ibuprofen, a nonsteroidal antiinflammatory drug. examination of the resected diverticulum revealed fundal-type gastric mucosa with inflammation, submucosal hemorrhages, erosions and ulceration in the absence of helicobacter pylo ... | 1992 | 1415106 |
| source of helicobacter pylori infection: studies in abattoir workers and pigs. | | 1992 | 1415120 |
| decreased level of antibodies against helicobacter pylori in patients with rheumatoid arthritis receiving intramuscular gold. | sodium aurothiomalate has been reported to have in vitro activity against helicobacter pylori. intramuscular gold, as given to patients with rheumatoid arthritis (ra), may therefore influence the colonisation of the gastric mucosa with h pylori. | 1992 | 1417132 |
| effect of helicobacter pylori lipopolysaccharide on the synthesis of sulfated gastric mucin. | the effect of h. pylori lipopolysaccharide on the synthesis and secretion of sulfated mucus glycoprotein in gastric mucosa was investigated. the lipopolysaccharide, while showing no discernible effect on the apomucin synthesis, exerted a profound inhibitory effect on the process of mucus glycoprotein glycosylation and sulfation, and evoked a rapid (within 15 min) inhibition (65%) in both mucin glycosylation and sulfation at its optimal concentration of 100 micrograms/ml. the data on mucin secret ... | 1992 | 1417902 |
| experience with 'triple' anti-helicobacter pylori eradication therapy: side effects and the importance of testing the pre-treatment bacterial isolate for metronidazole resistance. | at the 1990 world congresses of gastroenterology, the working party on helicobacter pylori (h. pylori) recommended that, in suitable patients, the bacterium should be eradicated using a therapeutic regimen comprising a bismuth salt, tetracycline and metronidazole for two weeks. we have treated 40 patients infected with h. pylori with 'triple' therapy consisting of 120 mg tripotassium dicitrato bismuthate q.d.s., 500 mg tetracycline q.d.s. and 400 mg metronidazole t.d.s. for two weeks. the succes ... | 1992 | 1420735 |
| short report: cefprozil for the eradication of helicobacter pylori infection. | helicobacter pylori infection has proven to be extraordinarily difficult to eradicate. antimicrobial monotherapies have been particularly disappointing, with most eradication rates in the range of 0 to 15%. we evaluated cefprozil (250 mg q.d.s. for 14 days) in 12 h. pylori-infected subjects. the 13c-urea breath test was used to evaluate effectiveness of therapy. eradication was defined as a negative urea breath test 4 to 6 weeks after the end of treatment. suppression of h. pylori was demonstrat ... | 1992 | 1420742 |
| clinicopathological assessment of gastric biopsy samples of patients with helicobacter pylori infection--metronidazole resistance and compliance problems in the united arab emirates. | the significance of helicobacter pylori (hp) infection was assessed prospectively in forty-two patients with dyspepsia using histological, bacteriological and biopsy urease techniques. thirty-eight patients (90.5%) were positive for hp infection and were treated with bismuth subcitrate (de nol), tinidazole and doxycycline. hp was present in the antrum, corpus, fundus, duodenum and gastric juice in 36, 26, 23, 2 and 2 patients respectively (p < 0.01, x2 test). histological assessment yielded more ... | 1992 | 1421445 |
| prevalence of gastritis in patients with acromegaly: untreated and during treatment with octreotide. | it has previously been suggested that acromegalic patients treated with the somatostatin analogue octreotide invariably have chronic gastritis. we have examined the prevalence of gastritis in a large group of acromegalic patients, untreated and during treatment with octreotide. | 1992 | 1424205 |
| [helicobacter pylori-associated gastritis and low-malignancy malt lymphoma]. | | 1992 | 1425268 |
| [helicobacter pylori: the local iga response of the gastric mucosa]. | | 1992 | 1425270 |
| [helicobacter pylori and gastric carcinoma]. | | 1992 | 1425302 |
| evaluation of a monoclonal antibody for detection of helicobacter pylori in a direct immunofluorescence test. | a monoclonal antibody was developed for detection of helicobacter pylori in gastric tissue sections in a direct immunofluorescence test. on a comparison of the immunofluorescence test with standard methods for detection of helicobacter pylori, i.e. culture, the urease activity test and histological examination of tissue sections, using 158 biopsy specimens, 30 specimens were positive in all methods and 64 negative. in the remaining cases comparison was not possible because either immunofluoresce ... | 1992 | 1425734 |
| surface hydrophobicity of gastric mucosa in helicobacter pylori infection: effect of clearance and eradication. | surface hydrophobicity of the gastric mucosa is reduced in peptic ulcer disease and helicobacter pylori infection. this abnormality may be caused by h. pylori or may be an inherent defect. the aim of the present study was to clarify the relationship between h. pylori infection and mucosal hydrophobicity by examining the effect of eradication of the organism. h. pylori-positive patients with (n = 42) or without (n = 42) duodenal ulcer were randomized to receive ranitidine, bismuth, or bismuth plu ... | 1992 | 1426866 |
| chronic active gastritis due to helicobacter pylori in immunized gnotobiotic piglets. | in gnotobiotic piglets, parenteral vaccination with formalin-killed helicobacter pylori and oral vaccination with live bacteria induced h. pylori-specific serum immunoglobulins g, m, and a. vaccination reduced but did not prevent infection by subsequent challenge with viable h. pylori. oral vaccination with killed bacteria was less effective in inducing serum antibody and had no effect on bacterial colonization. immunization status influenced the histologic response of piglets to challenge by h. ... | 1992 | 1426878 |
| eradication of helicobacter pylori reduces gastric and duodenal ulcer recurrence. | | 1992 | 1426890 |
| mucosal peptic activity during helicobacter pylori infection in pediatric patients. | intramucosal peptic activity may participate in the genesis of acute and chronic superficial gastritis. the proteolytic activity of homogenates of gastric mucosa (antrum and body) and duodenum were measured at ph 2.0 (total peptic activity) after exposure to ph 8.0 (pepsinogen) and the activated pepsinogen (pepsin) was calculated in pediatric patients investigated for the presence of helicobacter pylori (h pylori), 122 antral, 77 stomach body, and 74 duodenal biopsies were examined in 43 h pylor ... | 1992 | 1427366 |
| helicobacter pylori and peptic ulcer recurrence. | | 1992 | 1427384 |
| helicobacter pylori and peptic ulcer recurrence. | | 1992 | 1427385 |
| epithelial degeneration induced by helicobacter pylori. | | 1992 | 1427763 |
| does helicobacter pylori cause gastric cancer? | | 1992 | 1428029 |
| a study of helicobacter pylori in north indian subjects with non-ulcer dyspepsia. | a prospective case control study was conducted in 50 patients with non-ulcer dyspepsia (nud) and 10 age and sex matched controls to determine the prevalence of helicobacter pylori in patients with nud and to correlate symptoms, histology and presence of h pylori in gastric biopsies. endoscopic biopsies from antrum and fundus were subjected to urease test and histological examination. on histology, h pylori was identified in 27 (54%) nud patients and in one of 10 controls. urease test was positiv ... | 1992 | 1428036 |
| [dynamics of neutrophils in the gastric mucous layer of helicobacter pylori associated chronic gastritis]. | | 1992 | 1431391 |