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[myocarditis and pericarditis in connection with campylobacter jejuni infection].we describe a case of myo/pericarditis related to infection with c. jejuni. the microbe was repeatedly isolated from faeces and the patient had specific iga, igm and igg antibodies, detected in serum samples by means of a dig-elisa method. the condition is rare and the pathogenesis unclear. the case described may be the result of a primary infection or a reactive immune response.19921553694
[multicausal infectious respiratory tract disease of young fattening turkeys].the outbreak of the disease occurred in a large multiple-age farm with about 50,000 meat turkeys, where groups of 6-8000 one-day-old birds were stalled up every 14 days. all the turkey poults housed were affected mostly in the 1.-3. week of the life. the respiratory disease spread rapidly within the flocks and were characterised clinically by inclination of huddle, ruffled feathers, anorexia, stunted growth, swelling of the infraorbital sinus and nasal discharge. the clinical apparent disease la ...19921559465
a one-year study of enteric campylobacter infections in singapore.a total of 7344 inpatients with diarrhoea were investigated for campylobacter infections in singapore over one year. campylobacter species were recovered from 1.2% of diarrhoeal stools; 70.3% of the 91 campylobacters were from children under 5 years of age. further identification of these isolates showed that 89% were campylobacter jejuni and 10.9% were c. coli, and a high level of resistance to erythromycin was observed among them. concomitant isolation of salmonella species or shigella species ...19921560479
bilateral abducens paresis following campylobacter jejuni enteritis. 19921569314
campylobacter jejuni--an unusual cause of infectious arthritis. 19921570263
campylobacter enteritis in portugal: epidemiological features and biological markers.from 1984 to 1989, stool samples from 2811 gastroenteritis cases were examined for the presence of campylobacter jejuni and c. coli, salmonella, shigella and yersinia species. isolation rates were: campylobacter jejuni and c. coli, 5.3%, salmonella spp., 14.8%, shigella spp., 4.6% and yersinia enterocolitica, 1.1%. age group distribution analysis shows a higher campylobacter isolation rate in children under one year of age. seasonal distribution revealed a peak incidence in winter as in other me ...19921572427
[campylobacter jejuni diarrhea and myopericarditis]. 19921576305
phagocyte killing of campylobacter jejuni in relation to oxidative activation.when studying the interaction between campylobacter jejuni and human neutrophils, we found that four different clinical isolates showed a great variability in this association. also the ability to induce neutrophil production of oxidative metabolites, measured as chemiluminescence (cl), differed between the strains. surprisingly, strain 1, which showed weak interaction with neutrophils and high resistance to killing, induced the highest cl response. all strains evoked an intracellular cl respons ...19921586479
characterization of a 25,000-dalton helicobacter pylori protein, cross-reacting with a campylobacter jejuni protein.extract obtained by ultrasonic disruption of helicobacter pylori bacteria contained a protein with subunit molecular mass of 25 kd which bound antibodies in sera from patients with h. pylori-associated disease. the protein was purified by gel permeation and elution from sds-polyacrylamide gel slices, and was used to raise an anti-25-kd protein-specific rabbit serum. using the antiserum in experiments, the results indicated the following: the protein exists as covalently linked dimers (45 kd) of ...19921586485
a new case of peritonitis due to campylobacter jejuni in capd. 19921586699
conjugal transfer of antibiotic resistances and plasmids from campylobacter jejuni clinical isolates.six campylobacter jejuni clinical isolates were examined for the occurrence of plasmids in association with antibiotic resistances as well as conjugal transfer. all the isolates were found to carry three similar plasmids of 78 kb, 12.6 kb and 3.3 kb in size. multiple resistance to at least three of the antibiotics tested was observed with resistance to tetracycline most common. en bloc transfer of donor resistances at frequencies ranging from 10(-8) to 10(-4) were seen in all but one of the isol ...19921587459
cross-reactive proteins of borrelia burgdorferi.the specificity of serological tests for lyme borreliosis is impaired by cross-reacting antibodies. in order to select antigens for more specific tests, specific and cross-reactive proteins of borrelia burgdorferi must be identified. therefore, to analyze cross reactions of borrelia burgdorferi with other bacteria, rabbit immune sera against heterologous bacteria (borrelia hermsii, treponema pallidum, treponema phagedenis, leptospira interrogans (serogroup grippotyphosa), neisseria meningitidis, ...19921597198
age distribution of diarrhoeal and healthy children infected with campylobacter jejuni.the prevalence of infection with campylobacter jejuni in diarrhoeal and healthy children (age less than 7 years old) was investigated from 1 march to 31 may 1988 in chengdu city, china. the isolation rate in diarrhoeal children (11.88%) was higher than in healthy children (4.62%) (p less than 0.005). in both diarrhoeal and healthy children, infants (less than 12 months of age) had lower rates of infection than older children (p less than 0.005 and p less than 0.05 respectively). the frequency of ...19921597881
extended phage-typing scheme for campylobacter jejuni and campylobacter coli.the extended phage-typing scheme described for campylobacter jejuni and campylobacter coli has established 46 different phage types using 19 typing phages. altogether 754 campylobacter isolates, 672 c. jejuni and 82 c. coli, isolated from human and non-human sources received from 17 different countries were phage-typed. overall, 80.6% of the total isolates were typable. among typable strains, 9 phage types (3, 5, 10, 11, 18, 19, 23, 26 and 44) represented 57.0% of the strains, 21.3% of the strai ...19921601075
seroepidemiology of helicobacter pylori infection in vegans and meat-eaters.an enzyme-linked immunosorbent assay has been used to diagnose serologically the prevalence of helicobacter pylori infection in asian life-long vegans. there was no difference in the seropositivity between these individuals and a group of age- and sex-matched asian meat-eaters, indicating the meat consumption is not a risk factor for h. pylori infection. however, both asian groups had a higher prevalence of infection than age- and sex-matched caucasian meat-eaters. additionally, the asian indivi ...19921601079
microbic superinfection in relapse of inflammatory bowel disease.to assess the association between symptomatic relapse of inflammatory bowel disease (ibd) and superinfection with enteropathogenic microorganisms, we determined prospectively the incidence of infections with enteropathogenic bacteria, protozoa, and helminths in patients with confirmed longstanding ibd. sixty-four patients with ibd (49 with crohn's disease [cd] and 15 with ulcerative colitis [uc]) were consecutively enrolled in the study when relapse occurred. multiple biopsies for histological a ...19921607606
rectal bleeding caused by campylobacter jejuni in a neonate. 19921608692
reduction of campylobacter jejuni colonization of chicks by cecum-colonizing bacteria producing anti-c. jejuni metabolites.cecum-colonizing bacteria were isolated from campylobacter jejuni-free white leghorn (gallus domesticus) laying hens and screened for the ability to produce anti-c. jejuni metabolites. nine isolates were obtained that possessed this characteristic. the peroral administration of the nine isolates as a mixture (ca. 10(9) per chick) to 1-day-old chicks was followed 1 week later by peroral inoculation of campylobacter jejuni (ca. 10(9) per chick) to determine if the cecal isolates could protect chic ...19921610187
occurrence of thermotolerant campylobacters in fresh vegetables sold at farmers' outdoor markets and supermarkets.a total of 1564 fresh samples of 10 vegetable types from two different retail levels (533 samples from farmers' outdoor markets and 1031 samples from supermarkets) were surveyed for the occurrence of thermotolerant campylobacters. in samples from the outdoor markets, campylobacters were detected on six types of vegetables; the detection rates were spinach, 3.3; lettuce, 3.1; radish, 2.7; green onions, 2.5; parsley, 2.4; and potatoes, 1.6%. campylobacter jejuni was the predominant species (88%), ...19921611556
occurrence of campylobacter spp. in young gulls, duration of campylobacter infection and reinfection by contact.two groups of three week old herring gulls (larus argentatus) were held to observe the carrier state with campylobacter. all 27 birds of group i excreted campylobacter jejuni biotype iii when they were caught from their colony. four weeks later all but one were negative, indicating that the carrier state lasts until about the seventh week of life, with self-elimination if infection with another campylobacter species is prevented by housing in a closed environment as in this study. only one bird ...19921621473
[development of genetic and molecular approaches for the diagnosis and the study of the pathogenic power of helicobacter pylori, agent of inflammatory gastric diseases].helicobacter pylori (h. pylori) is a small gram negative bacillus, recently discovered, found in the stomach of patients with active chronic gastritis and duodenal ulcers. production of a potent urease has been described as a trait common to all h. pylori so far isolated. to clarify the role of urease in the pathogenic process, as well as to engineer genetic tools useful for the diagnosis of h. pylori, we cloned the genes responsible for urease activity. a genomic library was constructed in esch ...19921622109
study on the epidemiology and control of campylobacter jejuni in poultry broiler flocks.broiler flocks are frequently infected with campylobacter jejuni. the origin of the infection is still unclear. the question of whether colonization of flocks results from transmission of c. jejuni from breeder flocks to progeny (vertical transmission) or from environmental sources (horizontal transmission) remains to be answered. therefore, in this study samples were taken from successive broiler flocks in two broiler houses (house a on farm a and house b1 on farm b) as well as from the environ ...19921622265
campylobacter jejuni non-culturable coccoid cells.the behaviour of campylobacter jejuni in the environment is poorly documented. rapid loss of viability on culture media is reported. this phenomenon is associated with the development of so-called coccoid cells. it has been suggested that these cells can be infective to animals and man. results obtained with atp-measurements of coccoid cells and direct viable count (dvc) support this hypothesis. introduction of coccoid cells into simulated gastric, ileal and colon environments did not result in ...19921622752
distribution and numbers of campylobacter in newly slaughtered broiler chickens and hens.if campylobacter is present in the intestinal tract, broiler carcasses become extensively contaminated during the slaughter process. to determine the distribution and numbers of campylobacter jejuni/coli in newly slaughtered broiler chickens and hens, a total of 100 birds from six campylobacter-positive flocks were sampled at three swedish processing plants. campylobacters were isolated in 89% of neck skins, 93% of peritoneal cavity swab samples and in 75% of subcutaneous samples. muscle samples ...19921622758
outer membrane components of campylobacter hyointestinalis.outer membranes were isolated, by sodium lauryl sulphate extraction, from the american type strain, five australian, and four english isolates of campylobacter hyointestinalis. on sds-page examination, the protein profiles of seven strains (including the type strain) were similar, and were dominated by two major proteins of 47 and 50 kda. three other isolates had unique major protein profiles. the largest of these proteins was heat-modifiable in these isolates, and in the type strain. the flagel ...19921624127
variation in antigenicity and molecular weight of campylobacter coli vc167 flagellin in different genetic backgrounds.campylobacter coli vc167 has been shown to undergo a reversible flagellar antigenic variation between antigenic type 1 (t1) and antigenic type 2 (t2). vc167 contains two flagellin genes, and the products of both genes are incorporated into a complex flagellar filament in both antigenic types. although there are only minor amino acid changes in the flagellins expressed by t1 and t2 cells, the two antigenic types of flagellins can be distinguished by differences in apparent m(r) on sodium dodecyl ...19921624417
[serological studies on campylobacter jejuni/coli: establishment of national reference system for serological typing in japan].in order to establish a national reference system for campylobacter serotyping in japan, 7 local institutes of public health collaborated to prepare 30 serogrouping antisera including 26 antisera of lior's serogrouping system and 4 antisera of tck serogrouping system which was developed by the tokyo metropolitan research laboratory of public health. a total of 603 strains (92.2%) out of 654 isolates from 23 outbreaks of c. jejuni throughout japan were serogrouped by the 30 antisera. out of 1,198 ...19921624822
production of enterotoxin and cytotoxin in campylobacter jejuni strains isolated in costa rica.the production of toxins by 79 strains of campylobacter jejuni isolated in costa rica from children with campylobacter-induced diarrhoea (44 strains) and from chickens (35 strains) was studied. an enterotoxic effect giving a rounding of mouse adrenocortical tumour (y1) cells, which could be neutralised with antitoxin against escherichia coli heat-labile enterotoxin, was detected in supernates from 16 (62%) of 26 strains from children with watery diarrhoea, in 5 (28%) of 18 strains from children ...19921625311
factors that influence the interaction of campylobacter jejuni with cultured mammalian cells.although campylobacter jejuni is now recognised as a common enteric pathogen, the mechanisms by which this organism produces enteritis remain ill-defined. it has been proposed that its abilities to adhere to and enter epithelial cells represent properties essential to virulence. however, the characteristics of these interactions and factors that may influence the association of c. jejuni with epithelial cells are incompletely described. we have determined that the ability of c. jejuni to bind to ...19921625313
pathogenicity of campylobacter jejuni for turkeys and chickens.a campylobacter jejuni isolate obtained from a turkey liver, designated c101, and a c. jejuni isolate obtained from the feces of a chicken, designated c111, were used to inoculate their respective hosts. isolate c101 depressed weight gain by 20% when inoculated into newly hatched poults or 4-day-old poults. it also caused death, hepatic necrosis, and generalized hemorrhages in turkey embryos. the chicken-derived isolate, c111, did not reduce weight gain in newly hatched chicks, but it did induce ...19921627109
[an acute axonal polyneuropathy affecting intrinsic hand muscles following campylobacter infection--a case report].a 24-year-old carpenter had the shakes and fever on march 13, 1990. he suffered from watery diarrhea on march 14 and 15. he left muscle weakness in his thumbs and fingers when he drove nails with a hammer on march 24. the weakness reached maximum by the 3rd day of illness. he was admitted to our hospital on day 4. neurological examination revealed symmetrical weakness localized in the intrinsic hand muscles (mrc grade 2-4). the deep tendon reflexes were preserved. sensation was intact except for ...19921628453
immunoglobulin g antibodies to helicobacter pylori in patients with dyspeptic symptoms investigated by the western immunoblot technique.helicobacter pylori is a gram-negative, curved, rod-shaped bacterium known to cause gastritis and to be an important factor in the pathogenesis of peptic ulcers. serological testing has recently been proposed as an aid in diagnosis of h. pylori infections. in this study, we used the western immunoblot technique to evaluate the possibility of using one or more of the antigens from h. pylori for this purpose. thirteen major bands and about 30 minor bands could be identified by western blotting whe ...19921629330
acute campylobacter jejuni enteritis in 385 hospitalised patients.twenty healthy controls and 385 adult patients suffering from acute enteritis or gastroenteritis were enrolled for the study of campylobacter jejuni infection over a period of 2 years. thirty five stool samples showed c jejuni on stool culture. the isolation rates were at peak in the monsoon season and from watery and bloody stool specimens. pure c jejuni culture was obtained in 18 of 35 samples; the other 17 samples showed polymicrobial infection or infestation. nine of 35 patients were treated ...19921634481
translocation of campylobacter jejuni across human polarized epithelial cell monolayer cultures.the ability of campylobacter jejuni isolates to translocate across an epithelial cell barrier was investigated by using polarized caco-2 cell monolayers grown on microporous membrane filters. the 4 c. jejuni isolates tested all traversed the caco-2 cell monolayers and displayed similar translocation kinetics. the number of bacteria crossing the polarized cell monolayers continued to increase with time until 4 h after inoculation, at which time a maximum rate of translocation was observed. transm ...19921634802
guillain-barré syndrome associated with high titers of anti-gm1 antibodies.we found high titers of anti-gm1 antibodies (1/1280 or more) in 3 of 14 consecutive patients (21%) with guillain-barré syndrome (gbs) and in 2 additional patients who developed gbs, 10-11 days after starting parenteral treatment with gangliosides. antibodies were igg in 4 patients and igm in one, and they all bound to asialo-gm1, and, in 3, to gd1b as well. although the clinical features in all the patients with high anti-gm1 titers fulfilled the criteria for the diagnosis of gbs and in 4 of the ...19921634903
guillain-barré syndrome and campylobacter jejuni/coli. 19921641258
an outbreak of poisoning from penicillium cyclopium contaminated dried persimmon.it is the first report of an outbreak of 114 food-poisoning cases due to consumption of penicillium cyclopium contaminated dried persimmon. gastralgia, diarrhea, dizziness and general malaise are chief symptoms of the poisoning, with incubation period of 2-6 hrs generally and a short disease period (generally recovered within 2-3 days). no enteropathogenic organism, pathogenic coccus and campylobacter jejuni were detected. surface fungi counts were 49,000/g, 21.3 times of that discovered in the ...19921642784
the e-test and campylobacter jejuni.the e-test is a recently introduced method for performing antimicrobial susceptibility tests. we compared the e-test to the broth microdilution test and to the standard agar dilution test by using five antimicrobial agents tested against 55 clinical isolates of campylobacter jejuni from 11 locations in usa (group 1). later, we selected 30 strains (group 2), which were more resistant than the original survey isolates. erythromycin, tetracycline, and ciprofloxacin were tested on both groups of org ...19921643827
lack of colonization of 1 day old chicks by viable, non-culturable campylobacter jejuni.seven strains of campylobacter jejuni, isolated from various sources [human (n = 2), chicken (n = 3), water (n = 2)], were studied under starvation conditions in filter-sterilized and pasteurized surface water by acridine orange direct count (aodc), viable count (dvc) and culture methods. plate counts showed a rapid decline (2 log-units/day) for all strains under these conditions. only one of the seven strains (14%) showed a (prolonged) viable, non-culturable 'state'. the ability of these viable ...19921644708
[surveillance study on bacteriological etiology of 8371 children with diarrhea].the results of stool cultures from 8371 children of 0-13 years old with diarrhea during the years of january 1981 to december 1989 in shanghai area are reported. 2906 strains of enteropathogenic bacteria were isolated, with total detection rate of 34.72%. shigella were still the most common pathogens in 1981-1984, and campylobacter jejuni took second place, whereas from 1985 on campylobacter exceeded shigella as the first pathogenic bacteria of children's diarrhea. except in 1987 and 1988 shigel ...19911647837
characterization of high-level quinolone resistance in campylobacter jejuni.high-level resistance to quinolones has previously been shown to occur in campylobacter spp. both in vitro and in patients treated with quinolones. we have selected isolates that are resistant to quinolones by plating cells from a susceptible c. jejuni strain, ua535, on medium containing nalidixic acid at 32 micrograms/ml. fluctuation analysis indicated that resistance occurred by mutation at a frequency of 5 x 10(-8) per cell plated. unlike what is observed with other gram-negative organisms, t ...19911649570
clinical and serological studies in a series of 45 patients with guillain-barré syndrome.we retrospectively reviewed the clinical files of 45 guillain-barré syndrome (gbs) patients admitted to our department between 1979 and 1989. the age distribution was bimodal with a first peak in young adults (20-40 years), and a second one between 60 to 70 years. seasonal distribution showed a late fall and a hivernal predominance. three patients experienced a second attack of gbs 2-9 years after the first one. thirty-one (69%) presented antecedent events, most often a respiratory tract infecti ...19911655983
in vivo effects of sera from guillain-barré subgroups: an electrophysiological and histological study on rat nerves.serum from 20 patients with guillain-barré syndrome (gbs), 10 healthy controls and 10 patients with recent cytomegalovirus, epstein-barr virus, or campylobacter jejuni/coli infections was injected into rat sciatic nerve. the 20 gbs patients consisted of 2 groups of 10 patients with different electrophysiological and clinical disease patterns. the main aim of the study was to investigate possible differences in humoral (auto)-immunity between these subgroups. we found no statistically significant ...19911658645
in vitro susceptibilities of campylobacter jejuni and campylobacter coli to azithromycin and erythromycin.mics of azithromycin and erythromycin for 20 campylobacter coli and 20 campylobacter jejuni strains were determined. the results demonstrated that, for campylobacter species, all high-level erythromycin-resistant strains were also resistant to azithromycin and that azithromycin did not exhibit increased potency in comparison with that of erythromycin.19911659309
no signs of campylobacter jejuni/coli-related antibodies in patients with active ankylosing spondylitis.twenty-two patients with active ankylosing spondylitis were investigated to assess the levels of specific serum igg, iga and igm titres against campylobacter jejuni/coli before and during treatment with sulfasalazine. an enzyme-linked immunosorbent assay was used, and the results were compared with the antibody levels in 300 healthy blood donors. three patients had elevated levels of serum anti-campylobacter-iga before treatment, and a two-fold decrease in the antibody titre was observed during ...19911677583
dna restriction digest and ribosomal rna gene patterns of campylobacter jejuni: a comparison with bio-, sero-, and bacteriophage-types of united kingdom outbreak strains.dna restriction endonuclease (hae iii and hind iii) total digest and 16s and 23s ribosomal (r)rna gene patterns (ribopatterns) were determined for 18 isolates of campylobacter jejuni from three separate outbreaks of diarrhoea in the north of england. strains were also characterized by biotyping, serotyping and phage typing. comparisons of the dna patterns by visual and numerical methods revealed five distinct strain groupings with clear differences between isolates from different outbreaks as we ...19901698647
detection of antibodies to helicobacter pylori cell surface antigens.serum igg antibodies of helicobacter pylori were detected in single-dilution elisa using glycine extracted material. among 148 endoscopy patients 59% displayed antibodies; as expected, a higher occurrence (90%) was found in patients with positive gastric culture for h. pylori than in culture negative patients (37%). among 68 blood donors the frequency of h. pylori antibodies was 28%. in 73 children less than 15 years of age examined for unrelated disorders the occurrence was 4%. by immunoblottin ...19901699265
identification, characterization, and spatial localization of two flagellin species in helicobacter pylori flagella.flagellar filaments were isolated from helicobacter pylori by shearing, and flagellar proteins were further purified by a variety of techniques, including cscl density gradient ultracentrifugation, ph 2.0 acid disassociation-neutral ph reassociation, and differential ultracentrifugation followed by molecular sieving with a sephacryl s-500 column or mono q anion-exchange column, and purified to homogeneity by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transfer to an ...19911704004
revision of campylobacter, helicobacter, and wolinella taxonomy: emendation of generic descriptions and proposal of arcobacter gen. nov.hybridization experiments were carried out between dnas from more than 70 strains of campylobacter spp. and related taxa and either 3h-labeled 23s rrnas from reference strains belonging to campylobacter fetus, campylobacter concisus, campylobacter sputorum, campylobacter coli, and campylobacter nitrofigilis, an unnamed campylobacter sp. strain, and a wolinella succinogenes strain or 3h- or 14c-labeled 23s rrnas from 13 gram-negative reference strains. an immunotyping analysis of 130 antigens ver ...19911704793
localization of immunogenic regions on the flagellin proteins of campylobacter jejuni 81116.the purpose of this study was to localize antigenic regions on the flagellin protein of campylobacter jejuni 81116. this strain has two flagellin genes, flaa and flab, which are 95% identical; only flaa seems to be expressed in motile c. jejuni 81116 cells. fragments of flaa and flab were cloned in the bacterial expression vector pex, and the expression products were incubated with flagellin-specific antibodies. monoclonal antibodies to broadly cross-reactive epitopes recognized fragments that a ...19911705240
dna-dna hybridization and analysis of restriction endonuclease and rrna gene patterns of atypical (catalase-weak/negative) campylobacter jejuni from paediatric blood and faecal cultures.sixteen strains of atypical (catalase-weak or negative), hippurate-hydrolysing campylobacters from paediatric blood and faecal cultures were identified by dna-dna slot hybridization. all were closely related (greater than or equal to 67%) to campylobacter jejuni and representative strains had g + c contents of 30 +/- 1 mol%. numerical analysis of chromosomal dna haeiii digest patterns revealed two clusters of strains at the 55%s level corresponding to c. jejuni subsp. jejuni and c. jejuni subsp. ...19911707870
[the specificity of campylobacter jejuni antigens in immunoenzyme analysis].the surface antigen has been obtained from c. jejuni culture and its immunochemical analysis has been carried out by the methods of immunoelectrophoresis, double diffusion with homologous and heterologous rabbit sera. the protein profile of this antigen has been studied by means of vertical electrophoresis in polyacrylamide gel with sodium dodecyl sulfate, and the molecular weight of the protein components of the antigen has been determined. the presence of anti-campylobacter antibodies has been ...19901711263
cytotoxic effects of children's faeces: relation to diarrhoea due to clostridium difficile and other enteric pathogens.cytotoxicity of faecal extracts was demonstrated in 47 of 88 children (54%) referred for microbiological investigation of stools. cytotoxic clostridium difficile and vertotoxigenic escherichia coli (vtec) were the pathogens identified most commonly but cytotoxicity was also found in association with campylobacter jejuni, salmonella spp, shigella sonnei, giardia lamblia, rotavirus, adenovirus and poliovirus type 1 which had been acquired by oral immunization. in two patients, one of whom had cyst ...19911715139
evaluation of a simplified procedure for serotyping campylobacter jejuni and campylobacter coli which is based on the o antigen.a simplified procedure for serotyping campylobacter jejuni and campylobacter coli on the basis of thermostable antigens was developed and tested for its applicability as a routine typing method. the assay involves the sensitization of erythrocytes with an antigenic extract and performance of a slide agglutination assay with specific antisera. in order to simplify the typing system to a greater extent, the standard typing antisera were pooled into nine groups for c. jejuni and four groups for c. ...19911719020
specific serotype of campylobacter jejuni associated with guillain-barré syndrome. 19921727892
lysyl-trna synthetase gene of campylobacter jejuni.we report the cloning and complete nucleotide sequence of the campylobacter jejuni lysyl-trna synthetase gene (lyss). the c. jejuni lyss gene sequence shows high homology to the two escherichia coli lysyl-trna synthetase genes, lyss and lysu. the campylobacter lysyl-trna synthetase protein (lysrs) shows 47.9 and 46.6% sequence identity to the e. coli enzymes encoded by the lyss and lysu genes, respectively. the lysrs encoded by the c. jejuni gene is a polypeptide of 501 amino acids with a deduce ...19921732205
an outbreak of bacteremic campylobacter jejuni infection.during september 1980, an outbreak of bacteremic campylobacter jejuni infection occurred in metropolitan los angeles. the outbreak was recognized when blood cultures obtained from 11 previously healthy persons with acute febrile illnesses (characterized in over 80% by fever, diarrhea, and headaches) were positive for c. jejuni. all recovered after an illness that lasted a mean of 8 days. a surveillance system failed to reveal a concomitant outbreak of gastroenteritis. isolates had identical bioc ...19921734239
[development of genetic and molecular approaches for the diagnosis and study of the pathogenicity of helicobacter pylori, agent of gastric inflammatory diseases].helicobacter pylori (h. pylori) is a small gram-negative bacillus, recently discovered, found in the stomach of patients with active chronic gastritis and duodenal ulcers. production of a potent urease has been described as a trait common to all h. pylori so far isolated. to clarify the role of urease in the pathogenic process, as well as to engineer genetic tools useful for the diagnosis of h. pylori, we cloned the genes responsible for urease activity. a genomic library was constructed in esch ...19911742619
surveillance of patients attending a rural diarrhoea treatment centre in bangladesh.in may 1983, a surveillance system was set up at a rural diarrhoea treatment centre of the international centre for diarrhoeal disease research, bangladesh (icddr,b) to study a 20% systematic sample of all admissions. between may 1983 and april 1984, 2,635 patients were studied. a recognized enteric pathogen was detected in 69% of the patients screened, 15% of whom had a mixed infection. vibrio cholerae 0: 1 was the most common enteropathogen detected (39%), followed by enterotoxigenic escherich ...19911750109
a note on the comparative efficacy of three selective media for isolation of campylobacter species from environmental samples.the efficacy of three selective broths and agars, i.e. preston-, mccd- and car medium, were compared to each other in various combinations. twelve campylobacter coli and 9 campylobacter jejuni strains that had been isolated from sewage were used as test strains. to evaluate the applicability for highly contaminated environmental samples 15 sewage samples were examined, subsequently. significant differences between the media could not be ascertained. the application of preston broth/agar or car b ...19911750964
evaluation of media for primary isolation of campylobacter jejuni from faecal samples from children and animals.different media were used for primary isolation of campylobacter. butzler & preston medium was found to be more selective compared to skirows & blaserwang.19911752634
fluoroquinolone resistance in campylobacter jejuni and campylobacter coli isolated from human faeces in switzerland. 19911753149
potential sources of campylobacter species in the homes of farmworkers in zimbabwe.chicken faeces, houseflies, household stored drinking water, cooked or prepared foods and handwashings of people of all age groups and hands of children under 5 years old were examined as potential sources and transmission routes of campylobacter species in a farmworker community. campylobacter species were found to be common in chicken faeces collected from the homesteads of the farmworkers but were rarely isolated from houseflies or household stored drinking water. they were not isolated from ...19911758009
characterization of erythromycin resistance in campylobacter jejuni and campylobacter coli.the mechanism of resistance to erythromycin, the drug of choice in the treatment of campylobacter gastroenteritis, was investigated. erythromycin resistance (mics, greater than 1,024 micrograms/ml) in three clinical isolates of campylobacter jejuni and one c. coli isolate was determined to be constitutive and chromosomally mediated. in vivo protein synthesis in erythromycin-susceptible c. jejuni and c. coli strains was completely inhibited by low levels of erythromycin (5 micrograms/ml), whereas ...19911759819
emergence of fluoroquinolone resistance in campylobacter jejuni and campylobacter coli in subjects from finland.the in vitro susceptibilities of 102 human campylobacter strains isolated between 1978 and 1980 and 100 strains isolated in 1990 to ciprofloxacin, norfloxacin, erythromycin, gentamicin, and doxycycline were examined. the biotypes and heat-stable serotypes of the strains as well as antimicrobial treatments and travel history of the campylobacter-positive patients were also studied. the results indicated that susceptibility to erythromycin, gentamicin, and doxycycline has remained the same during ...19911759828
[comparison of clinical efficacy of rokitamycin (rkm) and ofloxacin (oflx) for the treatment of campylobacter enteritis by a double-blind method. the research committee for the effect of rokitamycin, research group for infectious enteritis].the clinical efficacy, safety and usefulness of rokitamycin (rkm), a new macrolide antibiotic, were compared with those of ofloxacin (oflx) for the treatment of campylobacter enteritis by a double blind method. the daily dose level of rkm or oflx was 600 mg. they were orally administered in three divided doses for 5 days. of 223 cases studied, 106 cases were diagnosed as campylobacter enteritis. ninety cases (rkm group: 50, oflx group: 40) except for 16 excluded or drop-out cases were analysed. ...19911761896
campylobacter fetus subspecies fetus infection.during a six-year period five patients with campylobacter fetus subspecies fetus infections were seen at the mayo clinic in rochester, minnesota. bacteremia was observed in two patients, one presenting with aortic valve endocarditis and the other with abdominal atherosclerotic aortic aneurysm. c. fetus subsp. fetus was isolated from tibial tissue of a patient with osteomyelitis. diarrhea was the main complaint of two further patients, and was also mentioned by the patient with the aortic aneurys ...19911762387
gastroenterological emergencies in the tropics.significant differences exist in the prevalence of most gastroenterological emergencies in tropical compared with temperate countries. both ethnic and environmental (often clearly defined geographically) factors are relevant. the major oesophageal lesions which can present acutely in tropical countries are varices and carcinoma; bleeding and obstruction are important sequelae. peptic ulcer disease (and its complications), often associated (not necessarily causally) with helicobacter pylori infec ...19911764626
[guillain-barré syndrome preceded by diarrhea with the infection of campylobacter jejuni].we report a 77-year-old woman with guillain-barré syndrome following campylobacter jejuni infection. she was admitted complaining of mild weakness in the left leg. seven days before, she had severe diarrhea, which continued several days. after admission, the weakness soon worsened resulting in tetraparesis, and the respiration became so impaired that she was supported by the artificial ventilator. deep tendon reflexes were absent in four limbs, and no sensory disturbance was noted. csf contained ...19911764866
rheumatic symptoms following an outbreak of campylobacter enteritis: a five year follow up.eighty six of 106 (81%) guests attending a party were followed up after an outbreak of campylobacter jejuni enterocolitis. acute diarrhoeal illness was reported in 35 subjects (33%), of whom seven showed acute rheumatic symptoms either alone or with other symptoms of infection with c jejuni. the antibody response to c jejuni corresponded well with the intensity of the disease. in the early phase of the gastrointestinal disease the patients with acute rheumatic symptoms displayed significantly hi ...19911768164
genomic investigation of phenotypic variation in campylobacter jejuni flagellin.western blots of whole-cell sonicates of 10 different clones of a faecal isolate of campylobacter jejuni 533 detected the expression of flagella antigens of either 59 or 62 kda. other antigenic proteins appeared identical both in the parent and all the clones. the mechanism for this phenotypic variation was studied using southern blotting with a flagellin-specific gene probe and products of a polymerase chain reaction (pcr) using flagellin-gene primers. restriction-enzyme digestion and southern ...19911769546
recovery of viable but non-culturable campylobacter jejuni.suspensions of campylobacter jejuni became non-culturable after storage in sterilized pond water at 4 degrees c for periods between 18 and 28 d, depending on the strain. suspensions of four strains of c. jejuni that had been in water for 6 weeks, and shown to be non-culturable, were fed to suckling mice. colonization of mice was established with two of the strains and failed with the other two strains. examination of these suspensions under the electron microscope showed some cocci having the ap ...19911770362
analysis of the role of flagella in the heat-labile lior serotyping scheme of thermophilic campylobacters by mutant allele exchange.flagellin mutations originally constructed in campylobacter coli vc167 (serotype lio8) by a gene replacement mutagenesis technique (p. guerry, s. m. logan, s. thornton, and t. j. trust, j. bacteriol. 172:1853-1860, 1990) were moved from the original host into campylobacter strains of a number of other lior serogroups by a natural transformation procedure. this is the first report of the use of this transformation method to transfer a mutated locus among campylobacter strains. flagellin mutants w ...19911774247
survival and injury of escherichia coli, campylobacter jejuni, and yersinia enterocolitica in stream water.experiments were done to describe the survival and injury of three strains each of enteropathogenic escherichia coli, yersinia enterocolitica, and campylobacter jejuni in agricultural surface water. bacterial suspensions within membrane diffusion chambers were immersed in two large vessels of stream water that were held at 6 and 16 degrees c and changed daily. the results of daily plate counts, using selective and nonselective media, revealed some variation among strains and genera. injury incre ...19911777856
development of spontaneous resistance to ciprofloxacin in a strain of campylobacter jejuni. 19911778865
recovery of injured campylobacter jejuni cells after animal passage.sixteen freeze-thaw-injured nonculturable stocks of campylobacter jejuni were passed through rat gut, and seven were reisolated. these reisolated strains were converted to toxin producers, as they were before preservation, following consecutive passages through rat gut. this observation indicated the existence of an injured, viable, but nonresuscitated form of c. jejuni which can be resuscitated to a culturable and fully virulent form by passaging the organism through a susceptible host.19911781696
electron microscopic appearance of the chronic campylobacter jejuni enteritis of mice.campylobacter jejuni is a major cause of human enteritis which mimics the inflammatory bowel disease (ibd). in this study, microstructural changes on the surfaces of the murine gastrointestinal tract persistently colonized by campylobacter jejuni, strain gj-s131, were investigated by using scanning electron microscopy (sem) and transmission electron microscopy (tem). the results revealed that the appearance of the gastrointestinal mucosa in both balb/c and km mice resembled that in human with in ...19911782809
isolation of campylobacter from livers of broiler chickens with and without necrotic hepatitis lesions.injured and normal livers from broiler chickens sent to slaughter plants were collected for bacterial examination. a total of 223 macroscopically abnormal livers and 50 normal livers were received. forty-seven thermophilic campylobacter isolates were obtained from the livers with necrotic lesions; 39 isolates were identified as campylobacter jejuni and eight as c. coli. in normal livers, six c. jejuni isolates were obtained. c. jejuni biotype 2 was the most common isolate recovered from injured ...19911786003
phagocytosis and intracellular killing of campylobacter jejuni by elicited chicken peritoneal macrophages.in vitro phagocytosis and intracellular survival of campylobacter jejuni (strains b540 and clin 1) in chicken peritoneal macrophages were studied. macrophages were induced with sephadex g-50 and harvested 48 hr later by peritoneal lavage. the extent of phagocytosis over time was determined by enumerating the intracellular c. jejuni after removal of extracellular c. jejuni with gentamicin. pre-incubation of c. jejuni with antiserum generally enabled the macrophages to ingest greater numbers of ce ...19911786008
childhood diarrhoea in a low-income urban community in bangkok: incidence, clinical features, and child caretaker's behaviours.a one-year surveillance study of childhood diarrhoea in a low-income urban community in bangkok revealed an annual incidence of 2.2 episodes per child among infants, and that the overall annual incidence among children under five years of age was 0.9 per child. rotavirus, salmonella and campylobacter jejuni were common aetiologic agents. in children less than one year, diarrhoea was caused mostly by rotavirus and salmonella. in 1-2 year old children, the major causative agent was rotavirus while ...19911787280
[in vitro antimicrobial activity of rokitamycin, a macrolide antibacterial agent, against clinically isolated strains of campylobacter and other enteritis-causing bacteria].we determined the minimum inhibitory concentrations (mics) of rokitamycin (tms-19-q, rkm), a macrolide antimicrobial agent, against strains of various bacterial species isolated from enteritis patients, and compared them with those of josamycin (jm), erythromycin (em) and ofloxacin (oflx). mic90 of rkm against 147 strains of campylobacter jejuni, and each 25 strains of shigella spp., salmonella spp. and diarrheagenic escherichia coli were 1.56, 200, 800 and 200 micrograms/ml, respectively. there ...19911791330
development of resistance to quinolones in five patients with campylobacteriosis treated with norfloxacin or ciprofloxacin.development of resistance to nalidixic acid, norfloxacin and ciprofloxacin was observed in five patients with campylobacter jejuni or campylobacter coli infection. from all these patients nalidixic acid- and quinolone-susceptible strains were isolated initially, whereas after therapy with norfloxacin or ciprofloxacin strains resistant to these antibiotics were found. campylobacter strains from the same patient always belonged to the same species and, with the exception of one case, showed identi ...19911794366
[bacterial ileocecitis: a "new" disease].in cases of clinical suspicion of an acute appendicitis sonography uncovers another disease as the real cause of the symptoms in about one-quarter of such cases. bacterial ileocaecitis is most frequently diagnosed (11.6% of n = 786). in special bacteriological stool cultures, yersinia enterocolitica and campylobacter jejuni were identified, whereas salmonella enteritidis was a rarer finding. the typical sonographic manifestation of bacterial ileocaecitis compared against crohn's disease of the i ...19911796286
acute diarrhoeal diseases in infants aged below six months in hospital in calcutta, india: an aetiological study.in a prospective 2-year study, 218 infants aged less than 6 months admitted to a children's hospital in calcutta with acute diarrhoea and 102 infants (control group) from the out-patient department of the same hospital with similar age, sex and socio-economic composition as the diarrhoea patients, were concurrently investigated for enteropathogenic agents in the faeces. enteropathogenic escherichia coli, enterotoxigenic e. coli, and enteroadherent e. coli constituted the 3 types of diarrhoeageni ...19911801358
[the adhesive properties of bacteria of intestinal origin].differences between strains of nonpathogenic escherichia and lactobacilli, as well as some pathogenic bacteria of enteric origin (escherichia, shigella, campylobacter), in their capacity to adhesion to rat enteric and colonic cells have been shown in vitro. the strains under study have been found to possess more pronounced adhesiveness with respect to colonic cells, which is indicative of their higher receptive capacity in comparison with enteric cells. in the absence of normal microflora lactob ...19911801491
[campylobacter jejuni--the etiologic agent of enteritis; the research experience within a closed collective of infants]. 19911802289
campylobacter jejuni infected bursitis.campylobacter jejuni is a common cause of enteritis, and has been isolated from patients with bacteremia, meningitis, and cholecystitis. we describe here an unusual case of a chronically inflamed bursitis infected with c. jejuni.19911802538
[morphological changes of campylobacter jejuni and campylobacter coli under various culture conditions and the accompanied changes in the cell composition].the morphological transformation from the spiral form to the coccoidal form campylobacter jejuni and campylobacter coli was studied under various conditions by such techniques as electron microscopy, sds-polyacrylamide gel electrophoresis (sds-page), and chemical analyses. the conversion from the spiral form to the coccoidal form of campylobacter in microaerophilic cultivation occurred in about 50% of the cells in 48 h and in about 90% of the cells in 72 h. at higher temperatures (37 c and 42 c) ...19911805043
[the effects of some technologies on survival of campylobacter jejuni in food of animal origin].the effects of some technologies (cooling, freezing, salting, packaging, fermentation, cold smoking, heat treatment) on the survival of campylobacter jejuni were investigated. the different technological procedures can be considered as barriers influencing to a certain degree the survival of c. jejuni. the low value aw on the surface of chilled meat and oxygen presence reduce the occurrence of c. jejuni, but the low storage temperature prolongs the survival of c. jejuni to two days. in chilled p ...19911807034
[an unusual cause of non-lithiasic acute cholecystitis: campylobacter jejuni]. 19911809764
the effect of lactic acid sprays on campylobacter jejuni inoculated onto poultry carcasses.spraying poultry carcasses with 1% lactic acid 10 min after inoculation with campylobacter jejuni, resulted in a significant reduction in the number of the bacteria after 4 h at 4 degrees c. some of the inoculated cells, however, survived for at least 144 h. spraying 10 min after inoculation with 2% lactic acid, totally eliminated all inoculated c. jejuni within 24 h. on the other hand, spraying 24 h after inoculation, with either 1% or 2% lactic acid did not eliminate all the bacteria. inoculat ...19911818508
[resistance to chemotherapy of campylobacter jejuni strains].sensitivity to 22 different chemotherapeutic agents against 31 campylobacter jejuni strains, isolated from children was determined. most of the investigated strains were sensitive to doxycycline, chloramphenicol and nitrofurantoin (90-100) and 84-87% of them to oxy-tetracycline augmentation, ofloxacin, erythromycin. all the strains were resistance to: cephalotin, cephadrin, cefuroxime and cefoperazone and 77-87% to ceftazidime, colistin and vancomycin.19911819812
[neonatal infection caused by campylobacter jejuni]. 19911826359
role of the beta-lactamase of campylobacter jejuni in resistance to beta-lactam agents.we studied the role of the beta-lactamase of campylobacter jejuni in resistance to beta-lactam agents. beta-lactamase-positive strains were more resistant than beta-lactamase-negative strains to amoxicillin, ampicillin, and ticarcillin (p less than 0.05). with penicillin g, piperacillin, imipenem, and six cephalosporins, the susceptibility levels were similar for both beta-lactamase-positive and -negative strains. by using spectrophotometric and microbiological assays, the beta-lactamase from th ...19911854162
enterotoxigenic campylobacter jejuni & c. coli in the etiology of diarrhoea in northern india.faecal specimens from subjects with (320) and without (450) diarrhoea were screened for campylobacter jejuni and c. coli. c. jejuni and c. coli were detected in 5 per cent of subjects with diarrhoea and 0.7 per cent of those without diarrhoea and the difference was significant (p less than or equal to 0.01). the isolation rate was much higher in under five diarrhoeal children (8.3%), in comparison to the older group (3.0%). eleven diarrhoeal isolates of c. jejuni and c. coli were tested in rat i ...19911855823
identification of campylobacter jejuni and campylobacter coli antigens with mucosal and systemic antibodies.the development of a rapid and specific diagnostic assay for campylobacter infections is important in determining the etiology of acute diarrhea in humans. studies have shown that sonicated whole bacteria or partially purified antigens cross-reacted with antibodies against other closely related bacteria. to solve the problems of specificity, we identified specific antigens of campylobacter jejuni and campylobacter coli for use in diagnostic assays. we investigated the responses of serum, urine, ...19911855976
iga nephropathy associated with campylobacter jejuni enteritis. 19911857464
interaction of cryptosporidium parvum and campylobacter jejuni in experimentally infected neonatal mice.by the method of scanning electron microscopy (sem), the inner mucosal surface of the ileum, ceacum and colon was studied in inbred balb/c mice. two-day-old mice were infected with either 10(6) oocysts of cryptosporidium parvum and 10(8) cfu of porcine and human strains of the bacterium campylobacter jejuni or with a combination of both enteropathogens. pathological changes in infection with c. parvum were related to enterocytes and villous atrophy appeared. in infection with c. jejuni, patholog ...19911863320
rates of detection of salmonella and campylobacter in meats in response to the sample size and the infection level of each species.pork, beef and chicken meat samples were collected from slaughter houses, poultry-processing plants and meat shops. rates of incidence of salmonella spp., campylobacter jejuni and c. coli with respect to the sample size were compared and the most probable number for these species were determined. salmonella spp. were detected in 69 (24.1%) of 286 chicken meat samples, in three (3.2%) of 94 pork samples, and in one (1.9%) of 52 beef samples. with chicken meat, the rates of detection were: 19.9% i ...19911863527
use of quinolones in the treatment of gastrointestinal infections.bacterial enteropathogens are responsible for between 40% and 80% of diarrheal illness depending upon the age of the persons affected and geographic areas where illness occurs. antibacterial agents will shorten the illness associated with enteric infection caused by enterotoxigenic escherichia coli, shigella spp. and campylobacter jejuni. these drugs also are effective in the therapy of certain clinical conditions (presumably because they are due to the same agents) which are characterized by mo ...19911864292
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