| ultrastructural studies of treponemes: location of axial filaments and some dimensions of treponema pallidum (nichols strain), treponema denticola, and treponema reiteri. | ultrathin sections of treponema pallidum (nichols strain), t. denticola (microdentium), and t. reiteri have been studied in the electron microscope to determine the location of the axial filaments and some of the dimensions of these organisms. the axial filaments of t. pallidum (nichols strain) have been seen to be tubular in cross section with an overall diameter of 21.0 +/- 0.73 nm, and an electron-lucent core of 8.0 nm. the filaments were found to lie on the outside of the organism which had ... | 1973 | 4570277 |
| amino acid and glucose fermentation by treponema denticola. | | 1971 | 4938689 |
| [studies on effects of treponema microdentium on cultured cells. i. effects of tissue culture medium 199 and eagle's minimum essential medium on the growth and survival of treponema microdentium]. | | 1965 | 5218829 |
| [electron microscopical studies on ultrathin sections of treponema microdentium]. | | 1966 | 5222883 |
| oxygen sensitivity of various anaerobic bacteria. | anaerobes differ in their sensitivity to oxygen, as two patterns were recognizable in the organisms included in this study. strict anaerobes were species incapable of agar surface growth at po(2) levels greater than 0.5%. species that were found to be strict anaerobes were treponema macrodentium, treponema denticola, treponema oralis n. sp., clostridium haemolyticum, selenomonas ruminatium, butyrivibrio fibrisolvens, succinivibrio dextrinosolvens, and lachnospira multiparus. moderate anaerobes w ... | 1969 | 5370458 |
| morphological and biochemical differentiation of three types of small oral spirochetes. | thirty strains of human oral anaerobic spirochetes were isolated in three different media: veal heart infusion-ascitic fluid, spirolate-brain heart infusion-rabbit serum, and supplemented pplo broth. the morphological and biochemical characteristics of the isolates permitted their differentiation into three distinct species: treponema denticola, t. macrodentium, and t. oralis (proposed new species). these species could be differentiated as follows. organisms of the t. denticola type had a "2-4-2 ... | 1969 | 5788714 |
| ultrastructure of treponema microdentium and borrelia vincentii. | bladen, howard a. (national institute of dental health, bethesda, md.), and edward g. hampp. ultrastructure of treponema microdentium and borrelia vincentii. j. bacteriol. 87:1180-1191.-a small oral treponema (fm) and borrelia vincentii (n9) were harvested after 3 to 7 days of incubation and either embedded in vestopal w or negatively stained with phosphotungstate. the protoplasmic cylinders of both strains were identical except for size, and had a triple-structured cell wall as well as intracel ... | 1964 | 5874540 |
| nutritional requirements of anaerobic spirochetes. i. demonstration of isobutyrate and bicarbonate as growth factors for a strain of treponema microdentium. | hardy, paul h., jr. (the johns hopkins university school of medicine, baltimore, md.), and carole o. munro. nutritional requirements of anaerobic spirochetes. i. demonstration of isobutyrate and bicarbonate as growth factors for a strain of treponema microdentium. j. bacteriol. 91:27-32. 1966.-the previously reported ability of a microaerophilic diphtheroid to support the growth of some oral spirochetes resides in the acid-distillable fraction of the culture fluid. this fraction can be replaced ... | 1966 | 5903097 |
| complement-fixing and hemagglutinating antibodies to treponema microdentium. | | 1967 | 6019894 |
| fatty acid requirement of treponema denticola and treponema vincentii. | treponema denticola and treponema vincentii were cultured in a medium supplemented with either 0.2 or 0.4% (w/v) alpha globulin in place of serum. the active factor(s) in alpha globulin was stable at ph 7.0 to autoclaving and was nondialyzable. extraction of lipids from alpha globulin showed that both protein and lipid, supplied by the alpha globulin, were required for maximal growth of these two oral treponemes. the lipid component was investigated by adding sodium salts of long-chain fatty aci ... | 1982 | 6177390 |
| detection of elevated serum antibodies to treponema denticola in humans with advanced periodontitis by an enzyme-linked immunosorbent assay. | | 1982 | 6212660 |
| enzymatic activities for interconversion of purines in spirochetes. | enzymatic activities that catalyze the interconversion of purines and purine derivatives were detected in cell extracts of spirochaeta aurantia, spirochaeta stenostrepta, treponema succinifaciens, and treponema denticola. phosphoribosyltransferase activities present in cell extracts of each of the four spirochete species functioned in the conversion of adenine, hypoxanthine, and guanine to amp, imp, and gmp, respectively. nucleotidase activities in the extracts mediated the formation of nucleosi ... | 1982 | 6292162 |
| demonstration of the in vitro phagocytosis of treponema denticola by human polymorphonuclear neutrophils. | a method has been developed for the study of phagocytosis of the oral treponeme t. denticola by human polymorphonuclear neutrophils. t. denticola was labelled with 32p-orthophosphate, and phagocytosis, expressed as counts per mg cell protein, was measured after 15, 60 and 120 min of interaction between neutrophils and treponemes. four different cell cultures were used as controls, and phagocytosis of e. coli was used as reference. the uptake of radiolabelled t. denticola was significantly larger ... | 1983 | 6362326 |
| scanning and transmission electron microscopy of the phagocytosis of treponema denticola and escherichia coli by human neutrophils in vitro. | in the present in vitro study, scanning and transmission electron microscopy demonstrated that human neutrophils are able to phagocytize treponema denticola cells. two major modes of particle engulfment were detected, both of which seemed unaffected by opsonization or atmosphere (aerobic or anaerobic). the occasional finding of rather intact treponemes as long as 2 h after onset of the phagocytosis experiment, suggested that digestion could be a relatively slow process. expulsion of digested mat ... | 1984 | 6382567 |
| neutrophil phagocytosis of treponema denticola as indicated by extracellular release of lactoferrin. | lysosomal enzyme release from viable human neutrophils occurs during phagocytic activity in vivo. phagocytosis of a strain of t. denticola, an oral spirochete, was indicated by the finding of lactoferrin in the extracellular medium of neutrophils challenged with this organism. the extracellular release of lactoferrin was dependent on duration of bacterial challenge, but peak concentration appeared at a later stage than seen in phagocytosis experiments with escherichia coli, which served as a con ... | 1984 | 6385616 |
| on the genesis of "dry socket". | one major school of thought regarding the pathogenesis of a dry socket occurring following tooth extraction is based on the concept that a blood clot fails to form, a concept that is, however, refuted by the clinical symptoms associated with the phenomena of a dry socket. a second theory maintains that, initially, clot formation takes place, but that the clot is subsequently lysed, bringing about the severe symptoms of a dry socket. fibrinolysis generated by tissue activators only partly explain ... | 1983 | 6579255 |
| attachment of treponema denticola to cultured human epithelial cells. | the present electron microscopic study visualizes adherence of treponema denticola, the most common treponeme of the gingival crevice in man, to human epithelial cells in vitro. the number of organisms adhering to the cells increased with increase in the treponemal concentration from 10(6) cells/ml through 10(8) cells/ml. epithelial cells immediately after mitosis were particularly rich in treponemes. the number of adsorbing treponemes was reduced after pretreatment of the epithelial cells with ... | 1984 | 6585912 |
| immunologic profile of juvenile periodontitis. i. lymphocyte blastogenesis and the autologous mixed lymphocyte response. | studies of blastogenesis of lymphocytes in culture from juvenile periodontitis (jp) patients have been inconclusive. experiments demonstrating differences in lymphocyte blastogenesis to preparations of putative periodontopathogens in jp and phytohaemagglutinin (pha) were performed. variables in the blastogenesis assay system recently reported were controlled which included using a range of cell and activator concentrations, incubation times of 3, 5 and 7 days, conical-bottomed microtest wells an ... | 1984 | 6592318 |
| albumin requirement of treponema denticola and treponema vincentii. | treponema denticola and treponema vincentii were found to require albumin, oleic acid, and thiamine pyrophosphate (tpp) for growth. previous studies indicated that commercial human alpha globulin, which is 50% albumin, was the only serum fraction that supported growth of these two oral treponemes. the alpha-globulin proteins were separated from the contaminating albumin with affi-gel blue affinity chromatography. both the albumin fraction and one of the alpha-globulin fractions were required for ... | 1983 | 6652579 |
| suppression of human lymphocyte responses by oral spirochetes: a monocyte-dependent phenomenon. | spirochetes have been implicated in the pathogenesis of several human infections including syphilis, yaws, lyme disease, and periodontal diseases. we examined soluble sonic extracts of oral spirochetes (treponema denticola and t. vincentii) for their ability to alter human lymphocyte function. these organisms were isolated from subgingival plaque of patients with periodontitis. we found that sonicates of several but not all strains of t. denticola caused a dose-dependent inhibition of human lymp ... | 1984 | 6699406 |
| suppression of fibroblast proliferation by oral spirochetes. | soluble sonic extracts of several strains of treponema denticola and treponema vincentii were examined for their abilities to alter proliferation of both murine and human fibroblasts. we found that sonic extracts of all tested strains of t. denticola caused a dose-dependent inhibition of murine and human fibroblast proliferation when assessed by both dna synthesis ([3h]thymidine incorporation) and direct cell counts. t. vincentii had only a minimal inhibitory effect at comparable doses. no inhib ... | 1984 | 6735466 |
| api zym system for identification of bacteroides spp., capnocytophaga spp., and spirochetes of oral origin. | a total of 80 oral strains of bacteroides gingivalis, b. asaccharolyticus, b. melaninogenicus subsp. intermedius, b. melaninogenicus subsp. melaninogenicus, capnocytophaga, treponema denticola, and t. vincentii were characterized with the api zym system for 19 enzyme activities. comparison of anaerobic and aerobic incubation with nine reference strains of these organisms showed no important differences. the key differential tests for black-pigmented bacteroides strains and treponemes of oral ori ... | 1982 | 6764781 |
| common antigens of treponema denticola: chemical, physical, and serological characterization. | a sodium deoxycholate-ethanol extractable antigen (des-ag) was obtained from four serotypes of treponema denticola and characterized by chemical, physical, and serological procedures. the cross-reactivity of these antigens was demonstrated by indirect microhemagglutination, immunodiffusion, and immunoelectrophoresis with hyperimmune rabbit antiserum against each of the t. denticola serotypes. antiserum against two nonoral treponemes, t. phagedenis biotype reieter and t. pallidum nichols strain, ... | 1982 | 6811433 |
| helical conformation of treponema pallidum (nichols strain), treponema paraluis-cuniculi, treponema denticola, borrelia turicatae, and unidentified oral spirochetes. | borrelia turicatae (mouse virulent) and treponema denticola, a small oral treponeme, formed right-handed helices as determined by scanning electron microscopy. treponema pallidum (nichols strain), treponema paraluis-cuniculi, and two unidentified oral spirochetes displayed left-handed helices. | 1981 | 7019083 |
| in vitro lymphocyte blastogenic responses and titers of humoral antibodies from periodontitis patients to oral spirochete isolates. | humoral antibodies and in vitro blastogenic responses of patients with moderate to advanced periodontitis and of orally healthy (control) individuals to 11 spirochetes were assayed. eight isolates of treponema denticola, two isolates of t. vincentii, and one isolate of t. phagedenis were grown anaerobically, sonicated, and centrifuged to remove cellular debris. the blastogenic responses of peripheral blood lymphocytes were measured by [3h]-thymidine uptake. antibody titers (immunoglobulins a, g, ... | 1982 | 7118245 |
| fumarate reduction and product formation by the reiter strain of treponema phagedenis. | the catabolic pathways for butyrate, acetate, succinate, and ethanol formation by the reiter strain of treponema phagedenis were investigated. enzyme activities were demonstrated for glucose catabolism to pyruvate by the embden-meyerhof-parnas pathway. butyrate formation from acetyl-coenzyme a (acetyl-coa) does not generate atp by substrate level phosphorylation and involves nad+-dependent 3-hydroxybutyryl-coa dehydrogenase and nad(p)+-independent butyryl-coa dehydrogenase activities. butyrate i ... | 1982 | 7142104 |
| pyruvate oxidation by the reiter strain of treponema phagedenis. | spectrophotometric assays of pyruvate oxidation catalyzed by extracts of the reiter strain of treponema phagedenis indicated that viologen dyes, flavin nucleotides, and a ferric iron chelate, but not pyridine nucleotides, were utilized as electron acceptors. benzyl viologen-linked activity partially sedimented during ultracentrifugation and appeared similar to clostridial pyruvate:ferredoxin oxidoreductase with respect to the spectral properties of the enzyme chromophore. electron carrier activi ... | 1982 | 7142105 |
| lymphoproliferative responses to oral bacteria in humans with varying severities of periodontal disease. | we assessed in vitro the lymphocyte blastogenic resp onsess of peripheral blood lymphocytes to antigen extracts of a large battery of oral microorganisms in a population of humans with varying severities of periodontal disease. when the magnitudes and frequencies of statistically positive blastogenic responses to various oral microorganisms were compared, three general patterns emerged. the actinomyces species proved to be potent stimulators of lymphocyte blastogenesis in most subjects tested, w ... | 1980 | 7399694 |
| immunochemical features of a macromolecule of treponema denticola. | in this study the extraction and the immunochemical features of a lipopolysaccharide-like (lpsl) macromolecule of t. denticola strains 35405, 35404, 33521 and 11 were investigated. the yield of lpsl molecule ranged between 0.5-0.9% of the cell dry weight, it possessed limulus amebocyte lysate clotting activity, and it contained glucosamine, phosphate, heptose, glucose, small amounts of kdo, myristic and beta hydroxy myristic acid. sera obtained from healthy individuals (ada type i) periodontitis ... | 1995 | 7473066 |
| effect of proteolytic enzymes on the lysis and growth of oral bacteria. | previous studies have shown increased levels of proteolytic enzymes in affected periodontal sites. the aim of the present investigation was to evaluate the effect of proteolytic environments on the lysis and growth of selected oral bacteria associated with either healthy or diseased periodontal sites. the effect of trypsin, chymotrypsin and proteinase k on cell lysis was determined following incubation with bacteria, whereas the effect of the same proteolytic enzymes on bacterial growth was test ... | 1994 | 7478762 |
| comparison of host tissue and bacterial dipeptidyl peptidases in human gingival crevicular fluid by analytical isoelectric focusing. | earlier work has shown that gingival crevicular fluid (gcf) contains dipeptidyl peptidase (dpp) activities that resemble those in host tissue. here, further comparisons were made with enzymes from suspected periodontal pathogens. gingival tissue and gcf were collected from patients with chronic periodontitis. dpp ii and dpp iv fractions with acid and alkaline ph optima, respectively, were separated from crude tissue extracts by gel-filtration chromatography. bacterial cell sonicates were prepare ... | 1995 | 7487574 |
| distribution of porphyromonas gingivalis and treponema denticola in human subgingival plaque at different periodontal pocket depths examined by immunohistochemical methods. | localization of porphyromonas gingivalis and treponema denticola in different areas of subgingival plaque from advanced adult periodontitis patients was studied immunohistochemically using sensitive immunogold-silver staining and immunoelectron microscopy. fourteen periodontally diseased teeth were extracted without damaging the subgingival plaque, fixed, and embedded. the subgingival plaque samples were sectioned according to four different pocket depths (0-2 mm, 2-4 mm, 4-6 mm and > or = 6 mm) ... | 1995 | 7494175 |
| an endo-acting proline-specific oligopeptidase from treponema denticola atcc 35405: evidence of hydrolysis of human bioactive peptides. | an endo-acting proline-specific oligopeptidase (prolyl oligopeptidase [popase], ec 3.4.21.26) was purified to homogeneity from the triton x-100 extracts of cells of treponema denticola atcc 35405 (a human oral spirochete) by a procedure that comprised five successive fast protein liquid chromatography steps. the popase is a cell-associated 75- to 77-kda protein with an isoelectric point of ca. 6.5. the enzyme hydrolyzed (optimum ph 6.5) the pro-pna bond in carbobenzoxy-gly-pro-p-nitroanilide (z- ... | 1994 | 7523301 |
| cloning and expression of a neutral phosphatase gene from treponema denticola. | we have isolated and characterized a neutral phosphatase gene, phon, from treponema denticola atcc 35405. the gene was isolated from a t. denticola clone bank constructed in the medium-copy-number plasmid vector pmcl19. subcloning and nucleotide sequencing of the dna insert from one phosphatase clone, ptph14, revealed that the activity corresponded to an open reading frame consisting of 1,027 bp coding for a 37.9-kda protein. hydrophobicity analysis indicated that the protein exhibits some hydro ... | 1995 | 7534273 |
| induction of cytoskeletal rearrangements and loss of volume regulation in epithelial cells by treponema denticola. | the early responses of oral epithelial cells to the adhesion of the oral spirochaete treponema denticola were studied as a model of microbial perturbation of the plasma membrane. kb cell (atcc ccl 17) monolayers were incubated with t. denticola (atcc 35405) in alpha-mem (minimal essential medium) for periods of 1-4 h at 37 degrees c without serum. control cultures were exposed to bacteria-conditioned alpha-mem without serum or bacteria or to alpha-mem alone. at the end of each incubation, detach ... | 1995 | 7541623 |
| role of the chymotrypsin-like membrane-associated proteinase from treponema denticola atcc 35405 in inactivation of bioactive peptides. | the ability of washed whole cells of treponema denticola atcc 35405 to hydrolyze (inactivate) substance p, bradykinin, and angiotensin i was studied. substance p was attacked primarily at the phe-8-gly-9 bond by a chymotrypsin-like proteinase (ctlp), at pro-4-gln-5 by an endo-acting prolyl oligopeptidase (popase), and at gln-5-gln-6 by an endopeptidase (falgpa-peptidase). bradykinin was cleaved at phe-5-ser-6 by the falgpa-peptidase and at pro-7-phe-8 by the popase. angiotensin i was rapidly con ... | 1995 | 7543886 |
| ribotyping on small-sized spirochetes isolated from subgingival plaque. | in the present study dna restriction patterns and corresponding ribotypes of 17 subgingival small-sized spirochetes (1:2:1 and 2:4:2 isolates), 2 treponema socranskii strains and two treponema denticola strains were examined. purified chromosomal dna was digested by bamhi, hindiii, psti and clai. the dna fragments were separated in a horizontal slab of 0.7% agarose containing ethidium bromide and transferred by nylon membranes. hybridization was carried out with digoxigenin-labelled copy dna of ... | 1995 | 7543994 |
| detection of putative periodontal pathogens in subgingival specimens by 16s ribosomal dna amplification with the polymerase chain reaction. | the utility of the 16s ribosomal rna-based polymerase chain reaction (pcr) for detection of actinobacillus actinomycetemcomitans, bacteroides forsythus, campylobacter rectus, eikenella corrodens, porphyromonas gingivalis, prevotella intermedia, and treponema denticola was examined and compared with that of anaerobic culture. primer pairs consisting of 20-27 nucleotides amplified 404- to 688-bp regions of 16s ribosomal rna genes of these organisms. this method had a lower detection limit of 50 ta ... | 1995 | 7548580 |
| oligonucleotide probes to the 16s ribosomal rna: implications of sequence homology and secondary structure with particular reference to the oral species prevotella intermedia and prevotella nigrescens. | eight oligonucleotides based upon regions of the small subunit 16s ribosomal rna gene sequences were analysed against a background of their position within the molecule and their two-dimensional structure to rationalise their use in recognising prevotella intermedia and prevotella nigrescens. the 41 clinical isolates from both oral and respiratory sites and two reference strains were subjected to dna-dna hybridisation and multilocus enzyme electrophoresis to confirm their identity. alignment of ... | 1995 | 7553378 |
| proteases of treponema denticola outer sheath and extracellular vesicles. | electron microscopical observations of the oral periodontopathogen treponema denticola show the presence of extracellular vesicles bound to the bacterial surface or free in the surrounding medium. extracellular vesicles from t. denticola atcc 35404, 50 to 100 nm in diameter, were isolated and further characterized. protein and proteolytic patterns of the vesicles were found to be very similar to those of isolated t. denticola outer sheaths. they were enriched with the major outer sheath polypept ... | 1995 | 7558307 |
| the 46-kilodalton-hemolysin gene from treponema denticola encodes a novel hemolysin homologous to aminotransferases. | the 46-kda hemolysin produced by treponema denticola may be involved in the etiology of periodontitis. in order to initiate a genetic analysis of the role of this protein in disease, its gene has been cloned. synthetic oligonucleotides, designed on the basis of the previously reported amino-terminal amino acid sequence of the 45-kda hemolysin, were used as primers in a pcr to amplify part of the hemolysin (hly) gene. this pcr product was then used to clone the entire hly gene from libraries of t ... | 1995 | 7591084 |
| intergeneric coaggregation of oral treponema spp. with fusobacterium spp. and intrageneric coaggregation among fusobacterium spp. | a total of 22 strains of treponema spp. including members of all four named human oral species were tested for coaggregation with 7 strains of oral fusobacteria, 2 strains of nonoral fusobacteria, and 45 strains of other oral bacteria, which included actinobacilli, actinomyces, capnocytophagae, eubacteria, porphyromonads, prevotellae, selenomonads, streptococci, and veillonellae. none of the treponemes coaggregated with any of the latter 45 oral strains or with the two nonoral fusobacteria. all ... | 1995 | 7591109 |
| cellular source, activation and inhibition of dental plaque collagenase. | dental plaque is the major aetiological factor in periodontal diseases and contains several proteolytic enzymes. the origin of these proteinases is, however, poorly studied. this study was undertaken to characterize collagenase present in dental plaque of adult periodontitis patients. vertebrate-type rather than bacterial-derived collagenase activity was detected in extracts of both supra- and subgingival dental plaque extracts of adult periodontitis patients. dental plaque collagenase was found ... | 1995 | 7593702 |
| identification of a flig homologue in treponema denticola. | using a bacteriophage lambda library of treponema denticola (td) atcc 35405 dna, and, as a reagent, sera derived from individuals with advanced adult periodontal disease, a variety of recombinant clones producing antigens of this oral spirochete have been isolated. nucleotide sequence analysis of a clone expressing three immunoreactive antigens has revealed the presence of an open reading frame highly homologous to the flagellar switch/motor protein, flig, which is known to be essential for flag ... | 1995 | 7642139 |
| cytopathic effects of treponema denticola chymotrypsin-like proteinase on migrating and stratified epithelial cells. | the effects of treponema denticola and its outer membrane-bound chymotrypsin-like proteinase on periodontal ligament epithelial cell cultures at different stages of maturity were studied. in sparse cultures with migrating epithelial cells, large intracellular vacuoles were formed rapidly following exposure to live t. denticola. treponemes showing structural damage were seen occasionally inside membrane-bound vesicles. intensive membrane blebbing occurred in infected cells and continued for up to ... | 1995 | 7642269 |
| distribution and characterization of plasmids in oral anaerobic spirochetes. | fifteen oral spirochete strains belonging to the species treponema denticola, treponema vencentii and treponema socranskii as well as 9 fresh clinical isolates were screened for the presence of extrachromosomal plasmid dna by a modified alkaline lysis procedure. a 2.6-kb plasmid was detected in both t. denticola atcc 33520 and t. denticola e'. the 2.6-kb plasmid from t. denticola e' was shown to be similar to ptd1, previously reported by ivic et al. in t. denticola atcc 33520 on the basis of mol ... | 1995 | 7644276 |
| capillary zone electrophoresis as a new tool in the chemotaxonomy of oral treponemes. | the existing taxonomy of oral treponemes is not satisfactory. this is due to the fact that these strict anaerobic bacteria are not easily cultivated or differentiated. therefore, new techniques that can contribute to improved cultivation, classification, and identification of these fastidious organisms should be welcomed. in the present study capillary zone electrophoresis (cze) was used to distinguish oral treponemes by their metabolic product patterns generated in a liquid medium. to our knowl ... | 1995 | 7663679 |
| evidence for two distinct locomotory phenotypes of treponema denticola atcc 35405. | using a two-layer system, a bottom layer containing treponema cells suspended in nos (new oral spirochete)-noble agar medium or nos-bacto agar medium and overlaid with cell-free nos-agarose medium resulted in the spirochete cells migrating into the top layer. however, if the positions of the medium layers were reversed with the cells inoculated into the bottom layer containing nos-agarose, there was no migration into the upper layer. this suggests migration of the spirochetes away from bacto and ... | 1995 | 7675518 |
| pore-forming properties of the major 53-kilodalton surface antigen from the outer sheath of treponema denticola. | a 53-kda protein from the outer sheath of the oral spirochete treponema denticola was purified to homogeneity and shown to reconstitute channels in black lipid bilayer model membranes. the channel had a single-channel conductance of 1.8 ns in 0.1 m kcl, making this the largest porin channel observed to date (estimated diameter, 3.4 nm). electron micrographs of 53-kda-protein-containing outer sheaths of t. denticola showed a regular hexagonal array of darker staining pits. | 1993 | 7682993 |
| homology among treponema denticola plasmids. | three of 16 isolates of treponema denticola were found to contain small (2.0-2.7 kb) cryptic plasmids. these were ptd1 from t. denticola atcc 33520, ptd2 from strain t32a, and ptd3 from strain d3a1. these plasmids were characterized by restriction mapping and cloned into e. coli plasmid puc19. extensive homology between these plasmids was revealed by southern blot, and single-stranded dna regions were found by neutral southern blots and s1 nuclease mapping. these plasmids were not found in serov ... | 1994 | 7808774 |
| effect of iron regulation on expression and hemin-binding function of outer-sheath proteins from treponema denticola. | the effect of the iron-chelating compounds edda and bpd on polypeptide regulation in the putative oral pathogen treponema denticola was studied. sds-page analysis of the t. denticola strains grown in the presence of edda or bpd, i.e. iron-limiting environmental conditions, revealed the expression of 44 and 43 kda polypeptides in the outer sheath, a 73 kda polypeptide in the cell membrane, and a 16 kda polypeptide in the soluble cell fraction. the hemin-binding activity of purified outer sheaths ... | 1994 | 7815916 |
| cloning and expression of hemolysin genes from treponema denticola strains atcc 35404 (td-4) and human clinical isolate gm-1 in escherichia coli. | the oral spirochete, treponema denticola is a putative etiologic agent in adult periodontitis, and acute necrotizing ulcerative gingivitis. in vitro, the oral treponeme produces several factors including proteases, hemolysins, hemin-binding proteins, which could potentially be involved in the virulence of this spirochete. our laboratory has been investigating the pathobiology of t. denticola, and has demonstrated the production of several hemolysins by t. denticola. in this report two hemolysin ... | 1994 | 7815917 |
| purification and general properties of an oligopeptidase from treponema denticola atcc 35405--a human oral spirochete. | an endo-acting oligopeptidase (opase) was purified to homogeneity from the cells of treponema denticola atcc 35405--a human oral spirochete--by a procedure that comprised a mild triton x-100 extraction (which disintegrates the outer membrane but leaves the cells morphologically intact) and four successive fast protein liquid chromatographic steps of the extract. the activity of this oligopeptidase (formerly named "trypsin-like" enzyme and "bana-peptidase") together with the proteinase activities ... | 1995 | 7864624 |
| physical map of the treponema denticola circular chromosome. | a physical map of the treponema denticola atcc 33520 genome was constructed by pulsed-field gel electrophoresis and dna hybridization. the organism possesses a single, circular chromosome of approximately 3.0 mbp and a 2.6-kbp circular plasmid, ptd1. the physical map of the a+t-rich genome was constructed with the rare-cutting restriction enzymes asci, noti, and srfi, which have 8-bp g+c-rich recognition sites. the genes flge, tdpa, and prtb encoding the flagellar hook protein, a 53-kda immunoge ... | 1995 | 7896704 |
| presence of treponema denticola and porphyromonas gingivalis in children correlated with periodontal disease of their parents. | considerable evidence exists suggesting that periodontal disease is due to the overgrowth of a finite number of specific bacteria such as porphyromonas gingivalis, actinobacillus actinomycetemcomitans, treponema denticola, bacteroides forsythus, and prevotella intermedia, among others. three of these organisms-p. gingivalis, t. denticola, and b. forsythus-can be easily detected in plaque samples by the hydrolysis of the synthetic trypsin substrate benzoyl-dl-arginine-naphthylamide (bana). the ai ... | 1994 | 7929977 |
| identification of lactoferrin-binding proteins from treponema pallidum subspecies pallidum and treponema denticola. | lactoferrin-binding or -associated proteins were identified in treponema pallidum subspecies pallidum and treponema denticola by affinity column chromatography using human lactoferrin and detergent-solubilized, radiolabelled spirochaetes. two discrete polypeptides of t. pallidum with masses of 45 and 40 kda and a broad band from 29-34 kda exhibited association with human apo- and partially ferrated lactoferrin. t. denticola produced two proteins that associated with a lactoferrin affinity matrix ... | 1994 | 7934885 |
| trypsin-like activity levels of treponema denticola and porphyromonas gingivalis in adults with periodontitis. | treponema denticola (td) and porphyromonas gingivalis (pg) are associated with human moderate and severe adult periodontal diseases. this study quantifies these two anaerobes and their trypsin-like (tl) activities in subgingival plaque collected from both clinically healthy and periodontally diseased sites of human periodontitis patients. antigen levels of the microorganisms were determined by monoclonal antibodies and tl activities were measured by the fluorescent substrate z-gly-gly-arg-afc in ... | 1994 | 7989615 |
| degradation of endogenous plasma membrane fibronectin concomitant with treponema denticola 35405 adhesion to gingival fibroblasts. | treponema denticola adhesion and degradation of fibronectin (fn) on human gingival fibroblasts (hgf) were studied by immunofluorescence and enzyme-linked immunosorbent assays. the number of adherent bacteria increased and the amount of immunoreactive fn decreased as a function of increasing t. denticola concentration. the distribution of cell-bound fn was punctate in micrographs. anti-human fn impaired bacterial adhesion to hgf. phenylmethylsulfonyl fluoride inhibited fn degradation but not adhe ... | 1994 | 8005694 |
| treponema denticola as a model for polar adhesion and cytopathogenicity of spirochetes. | recent studies suggest that the tip-oriented adhesion of treponema denticola is a consequence of polar clustering of its adhesins on contact with the substratum and is compatible with viability. such adhesion to cells stimulates a host of cytoskeletal and volume regulatory changes. these studies may give insight into mechanisms of colonization and cytopathogenicity of other pathogenic spirochetes. | 1994 | 8012753 |
| cytotoxic effect of peptidoglycan from treponema denticola. | peptidoglycan isolated from pathogenic bacteria has been previously found to exhibit various biological activities. the aim of this investigation was to evaluate the toxicity of treponema denticola peptidoglycan towards epithelial cells. the cytotoxicity of a lipopolysaccharide-like material was also determined. epithelial cells were incubated with the test substances and cell viability was assayed by measuring lactate dehydrogenase activity and the conversion of tetrazolium salt into blue forma ... | 1993 | 8015419 |
| cloning and sequence analysis of a chymotrypsinlike protease from treponema denticola. | a clone expressing a treponema denticola chymotrypsinlike protease from recombinant plasmid psa2 was identified in a genomic library of t. denticola atcc 35405. nucleotide sequencing of the insert identified an open reading frame, designated the prtb gene, which codes for the protease. two potential inverted repeat sequences are present both upstream and downstream from the prtb gene. the prtb gene would code for a putative protein of 273 amino acids with a calculated molecular mass of 30.4 kda ... | 1994 | 8039913 |
| characterization of hemolysis and hemoxidation activities by treponema denticola. | this study determined the presence of two hemolytic activities in the oral treponeme, treponema denticola, strains atcc 35404 (td-4), atcc 33520, gm-1, and ms25. these activities, referred to as hemolytic and hemoxidative (hea, heo, respectively), were found to be both secreted into the extracellular environment, and cell associated. the extracellular activity was associated with small molecules with relative molecular weights of < 1000 da, and its activity was cysteine independent; the cell-ass ... | 1994 | 8090077 |
| purification and characterization of a 45 kda hemolysin from treponema denticola atcc 35404. | a 45 kda polypeptide capable of erythrocyte (rbc) lysis and hemoglobin oxidation was isolated from treponema denticola, atcc 35404 (td-4) after sequential ammonium sulfate (2.8-3.6 m) precipitation and preparative electrophoresis. the purified polypeptide produced a single protein band on page at a relative molecular weight of 45 kda in the presence and absence of sds. the polypeptide was sensitive to proteinase k and pronase, and heating at 80 degrees c. the protease inhibitors, pmsf, tlck and ... | 1994 | 8090078 |
| isolation and characterization of a 53 kda major cell envelope protein antigen from treponema denticola atcc 35405. | a major cell envelope protein was purified from the cell envelope fraction of treponema denticola atcc 35405 by ion exchange chromatography after extraction with zwittergent 3-14. sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed a relative molecular mass of 53 kda for this protein with a pi of 6.3-6.8. amino acid analysis revealed that this protein contained high proportions of hydrophobic amino acids (40.4%), and no cysteine could be detected. the n-terminus of the protein was b ... | 1994 | 8113954 |
| similarity between the 38-kilodalton lipoprotein of treponema pallidum and the glucose/galactose-binding (mglb) protein of escherichia coli. | the recent discovery that abundant and immunogenic lipoproteins constitute the integral membrane proteins of treponema pallidum has prompted efforts to investigate their importance in the physiology and ultrastructure of the organism and in immune responses during infection. earlier studies identified a 38-kda lipoprotein of t. pallidum believed to be specific to the pathogen. in the present study, monoclonal antibodies generated against the 38-kda lipoprotein of t. pallidum reacted with cognate ... | 1994 | 8132345 |
| detection of porphyromonas gingivalis in gingival exudate by a dipeptide-enhanced trypsin-like activity. | porphyromonas gingivalis in subgingival plaque is an important risk factor for future periodontal attachment loss in susceptible adults. the elimination of p. gingivalis is usually concomitant with a healing process. therefore, it should be valuable to have an easy chairside method to follow the effect of periodontal treatment on p. gingivalis detection as well as on its eventual reappearance during the maintenance period. we have previously reported the stimulation of amidolytic activity of p. ... | 1994 | 8133415 |
| characterization of antisera raised against treponema denticola (atcc 33521) whole cell, outer sheath, protoplasmic cylinder, and axial flagella. | in this study we produced polyclonal antisera directed to whole cell, outer sheath, protoplasmic cylinder and axial flagella sonicates of treponema denticola (atcc 33521) reference strain. furthermore, the reactivity of the antisera was determined, using the enzyme-linked immunosorbent assay and immunoblotting techniques. as control antigen, other gram-negative bacteria (salmonella minnesota, escherichia coli) and related pathogenic spirochetes (borrelia burgdorferi and treponema pallidum) were ... | 1994 | 8167426 |
| characterization of fibrinolytic activities of treponema denticola. | several fibrinolytic activities of treponema denticola, an oral spirochete associated with gingivitis and periodontal disease, were identified and characterized following phase partitioning with the nonionic detergent triton x-114. the apparent molecular masses of the proteases ranged from 91 to 228 kda when analyzed in sodium dodecyl sulfate-polyacrylamide gels containing fibrinogen as the protease substrate. a qualitative analysis of zymograms showed that the proteases were highly enriched in ... | 1994 | 8168936 |
| diversity of cultivable and uncultivable oral spirochetes from a patient with severe destructive periodontitis. | to determine the genetic diversity of cultivable and uncultivable spirochetes in the gingival crevice of a patient with severe periodontitis, partial 16s rrna genes were cloned from pcr-amplified products of dna and rna extracted from a subgingival plaque sample. approximately 500 bp were amplified in pcrs by using universally conserved primers with polylinker tails. purified pcr products were cloned into escherichia coli by using the plasmid vector puc19. the resultant clone library was screene ... | 1994 | 8168954 |
| antimicrobial action of axol on periodontopathic bacteria. | the antimicrobial action of axol was tested against a panel of periodontopathic bacteria, which included treponema denticola, treponema vincentii, treponema sp., porphyromonas gingivalis, prevotella intermedia, prevotella melaninogenica, and fusobacterium nucleatum. the axol commercial solution (undiluted) was effective in inhibiting some of the bacteria but not all. the rational for the use of antimicrobials is discussed. | 1993 | 8180485 |
| clustering of fibronectin adhesins toward treponema denticola tips upon contact with immobilized fibronectin. | treponema denticola has been shown to bind to immobilized fibronectin (fn) by its tips. yet labeling of cells in suspension with an fn-gold conjugate to localize the fn adhesins shows that they are distributed in patches along the entire cell length. subsequent experiments have shown that the number and proportion of tip-oriented cells increase with time, suggesting that fn contact stimulates t. denticola to rearrange adhesins toward its tips. to test this hypothesis, t. denticola cells were all ... | 1994 | 8188343 |
| development of quasi-multicellular bodies of treponema denticola. | the formation of quasi-multicellular bodies of treponema denticola was analysed using different electron microscopical methods. these bacteria could develop four different conformations: (i) normal helical forms; (ii) twisted spirochetes, forming plaits; (iii) twisted spirochetes,forming club-like structures; (iv) spherical bodies in different size. treponemes within spherical bodies, plaits, and clubs proved to be enclosed in a common outer sheath in which the normal arrangement of their axial ... | 1993 | 8215797 |
| configural frequency analysis for exploring bacterial sets in periodontal health and disease. | periodontal research has been concerned with identifying bacterial sets in oral health and disease. configural frequency analysis (cfa) is a taxonomical method used to test the statistical significance of patterns of elementary cell frequencies in cross-tabulated data, and may therefore be used to evaluate interactions among occurrences and non-occurrences of periodontal microorganisms. cfa patterns may exist as configural types or anti-types depending on whether observed cell frequencies are gr ... | 1993 | 8222602 |
| the effects of incubation length and temperature on the specificity and sensitivity of the bana (n-benzoyl-dl-arginine-naphthylamide) test. | a previous multi-center study examining patients diagnosed as having at least four periodontally diseased teeth showed that when bana (n-benzoyl-dl-arginine-naphthylamide) hydrolysis by periodontal pathogens such as treponema denticola, porphyromonas gingivalis, and bacteroides forsythus was evaluated versus clinical parameters such as clinical judgment of disease, bleeding on probing, and pocket depth, the sensitivity of the test was 84%, 82%, and 87%, respectively, while the specificity was on ... | 1993 | 8229620 |
| phosphorylated nutrient uptake by treponema denticola and other potential periodontopathogens. | nutrient uptake may contribute to the survival of certain potential periodontopathogens in the competitive environment of the gingival sulcus. this study was conducted to assess the uptake of commercially available, key radiolabelled phosphorylated compounds by treponema denticola. there was a linear relationship in the uptake of atp and g-1-p with cell concentrations of 0.1-2.5 mg of cell dry weight. this uptake reached a steady state within 15 min, and at temperatures of 25-37 degrees c the up ... | 1993 | 8229676 |
| involvement of treponemal surface-located protein and carbohydrate moieties in the attachment of treponema denticola atcc 33520 to cultured rat palatal epithelial cells. | we studied the nature of attachment of treponema denticola atcc 33520 to a microscopically distinct population of rounded rat palatal epithelial cells. the motility of the freshly harvested spirochetes appeared not be a prerequisite for attachment. treatment of t. denticola atcc 3350 with proteinase-k, heat, glutaraldehyde, formaldehyde and periodate oxidation decreased the attachment to the rounded rat palatal epithelial cells, indicating the involvement of protein and carbohydrate moieties. tr ... | 1993 | 8247611 |
| viscosity-dependent locomotion of oral spirochetes. | spirochetes are thought to remain motile in environments (such as intercellular spaces) that immobilize extracellularly flagellated eubacteria. this attribute suggests that the viscosity of the milieu is of importance to locomotion. we sought to determine the interdependence of oral spirochete locomotion with media viscosity. video time-lapse microscopy using darkfield optics was used. the motility of the spirochetes in media of different viscosities (various concentrations of noble agar) was me ... | 1993 | 8247612 |
| binding of hemin and congo red by oral hemolytic spirochetes. | colony-forming units or cells in suspension of oral anaerobic spirochetes (treponema denticola, treponema vincentii and treponema socranskii) bind hemin and congo red. hemin or congo red binds to a hydrophobic polypeptide receptor that is located in the outer membrane of the bacterial cells and it has a relative molecular mass of 47 kda. these oral spirochetes also lyse sheep erythrocytes to produce beta-hemolytic zones around colony-forming units. the oral spirochetes may acquire iron for growt ... | 1993 | 8247613 |
| proteolytic and oxidoreductase activity of treponema denticola atcc 35405 grown in an aerobic and anaerobic gaseous environment. | the cells of a human oral spirochete, treponema denticola atcc 35405, and of seven clinical isolates of this organism obtained from the subgingival dental plaque of periodontitis patients were studied for their ability to grow in an aerobic and an anaerobic environment, and for their profile of peptidohydrolase and oxidoreductase enzymes. the growth yield of aerobically grown cultures was either comparable to or higher than that of anaerobically grown ones regardless of whether prereduced broth, ... | 1993 | 8248625 |
| fatty acid profiles of the outer membrane of atcc strains 35405, 35404 and 33521 of treponema denticola. | the fatty acid composition of the outer membrane (outer sheath) of treponema denticola is not known. this study examined the fatty acid profiles of the outer membranes of t. denticola atcc strains 35405, 35404, 33521. homogeneous outer membranes were prepared from the three strains of t. denticola. the fatty acids were extracted and converted to methyl esters, and their mass spectra were determined with a sensitive hewlett-packard 5880a-5970 gas chromatography-mass spectrometry system. fatty aci ... | 1993 | 8254455 |
| common and specific antigens of several treponemes detected by polyclonal antisera against major cellular proteins. | thirteen polypeptide antigens with molecular weights ranging from 34 kda to 83 kda were selected and their antigenic behaviors and distribution were examined in 12 strains of microorganisms including treponema, borrelia, leptospira and leptonema. immunoblot analysis demonstrated that 45 kda and 83 kda polypeptides of treponema socranskii subsp. buccale atcc 35534, 53 kda antigen of treponema denticola atcc 33520 and 44 kda polypeptide of the strain g7201 were strain-specific. the 34, 62, 66 and ... | 1993 | 8265202 |
| outer membrane antigens of oral treponema species. | outer membrane (om) antigens of several oral treponemes were studied. sds-page revealed a 56-58-kda protein as a major component of isolated om vesicles. immunoblot analysis with eluted antibodies prepared from treponema denticola atcc 33520 recognised the 56-58-kda protein, which was highly conserved in the 17 t. denticola strains tested. the protein was also a component of a t. denticola desoxycholate-extractable, ethanol-soluble antigen (des-ag) but was not present in t. pectinovorum or t. vi ... | 1994 | 8289216 |
| dna probe detection of periodontopathogens in advanced periodontitis. | species-specific dna probes were used to determine the presence of actinobacillus actinomycetemcomitans (a.a.), porphyromonas (bacteroides) gingivalis, prevotella intermedia, treponema denticola, eikenella corrodens, fusobacterium nucleatum, and wolinella recta in subgingival plaque from deep pockets/sites of patients with advanced periodontitis. the subjects were 20 patients with severe adult periodontitis, 13 men and 7 women (mean age 45.6 +/- 6.7 yr). for each subject, 9-10 subgingival sites ... | 1993 | 8290878 |
| bacterial mediators in periodontal disease. | periodontal disease is the general description given to the inflammatory response of the gingiva and underlying connective tissue to bacterial accumulations (dental plaque) on the teeth. a limited number of cultivable species are usually associated with periodontal disease. the majority of putative periodontal pathogens are gram-negative anaerobic rods. some of the characteristics of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and treponema denticola will be discussed, given ... | 1993 | 8324120 |
| effect of outer membrane of treponema denticola on bone resorption. | the effect of the outer membrane (outer sheath) of treponema denticola on bone resorption was studied. bone resorption was measured by the release of previously incorporated 45ca from the shafts of the radii and ulnae of 19-day fetal rats. a treated-over-control ratio (t/c ratio) significantly greater than 1 indicated the stimulation of bone resorption by the test substance. the addition of outer membrane of t. denticola increased the release of 45ca from the assay bones. the minimum concentrati ... | 1993 | 8355985 |
| a successful method for quantifying viable oral anaerobic spirochetes. | spirochetes are markedly prevalent in periodontal disease but are not included as predominant cultivable organisms because of the inability to quantify them by viable count. a successful method was developed for enumerating viable oral spirochetes as colony-forming units (cfu) in an agarose-based medium. treponema denticola, treponema vincentii and treponema socranskii in log-phase growth in new oral spirochete (nos) broth were used for evaluation of the method. critical components of the method ... | 1993 | 8355989 |
| attachment of treponema denticola strains to monolayers of epithelial cells of different origin. | the attachment of 10 different treponema denticola strains to monolayers of 4 types of epithelial cells derived from rat palatal epithelium, guinea pig ear, human buccal epithelium and human corneal epithelium was screened microscopically. most t. denticola strains were able to attach to all four types of epithelial cells. the t. denticola strains seemed to attach better to epithelial cells derived from primary cultured material. the t. denticola strains showed different degrees of attachment. s ... | 1993 | 8355990 |
| stimulation of in vitro growth of treponema denticola by extracellular growth factors produced by porphyromonas gingivalis. | cell-free culture filtrates of porphyromonas gingivalis grown in wilkins-chalgren broth stimulated the growth of six strains of treponema denticola in 1186 broth when compared with the effect of uninoculated wc. the ph of the 1186 broth was not altered by the addition of either culture filtrate or wc, and all media were fully reduced prior to inoculation with t. denticola. growth was also stimulated by factors precipitated from the culture filtrate with 90% (nh4)2so4, 50% cold ethanol, or 50% co ... | 1993 | 8388413 |
| prevalence of treponema denticola and porphyromonas gingivalis in plaque from periodontally-healthy and periodontally-diseased sites. | intracrevicular plaque from periodontally-healthy individuals who had refrained from oral hygiene measures for 24 h prior to sampling, and subgingival plaque from diseased sites of patients with chronic periodontitis were screened by elisa for the presence of porphyromonas gingivalis and treponema denticola. the samples were also subjected to the perioscan test to detect the presence of enzymes capable of degrading n-benzoyl-dl-arginine-2-naphthylamide (bana). of the 141 samples from periodontal ... | 1993 | 8389879 |
| the effect of incubation temperature on the specificity of the bana (n-benzoyl-dl-arginine-naphthylamide) test. | the hydrolysis of bana by subgingival plaque samples is associated with the presence of either treponema denticola, porphyromonas gingivalis, and/or bacteroides forsythus. a protocol in which pure cultures were incubated for 15 min at 55 degrees c detected about 5 x 10(5) cfu of p. gingivalis and 1 x 10(6) cfu of t. denticola. clinical studies indicated that the bana test in this configuration will detect about 10(4) organisms in vivo as compared with the 10(5) to 10(6) organisms found with in v ... | 1993 | 8390044 |
| measurement of electrical bioimpedance for studying utilization of amino acids and peptides by porphyromonas gingivalis, fusobacterium nucleatum, and treponema denticola. | the growth response of porphyromonas gingivalis, fusobacterium nucleatum, and treponema denticola to peptides (supplied as trypticase) and amino acids (supplied as casamino acids) was measured over 24 hours by monitoring changes in alternating-current conductivity at 37 degrees c. all species utilized peptides preferentially over amino acids. these results are consistent with those obtained previously with conventional growth-response experiments. the duration of the linear growth response to tr ... | 1993 | 8391864 |
| the effects of acacia arabica gum on the in vitro growth and protease activities of periodontopathic bacteria. | the antibacterial activity of acacia gum was assessed using fresh isolates and reference strains of actinobacillus actinomycetemcomitans, capnocytophaga spp., porphyromonas gingivalis, prevotella intermedia and treponema denticola. a fine aqueous suspension of gum was produced by sonication and then a soluble fraction isolated by centrifugation and membrane filtration. these preparations were incorporated into columbia agar at doubling concentrations. growth of p. gingivalis and p. intermedia cu ... | 1993 | 8473532 |
| fibronectin-binding proteins of a human oral spirochete treponema denticola. | major polypeptides from a human oral spirochete treponema denticola atcc 33520 were examined to demonstrate their ability to bind to human plasma fibronectin by immunoblot analysis. of three main polypeptides separated on sodium dodecyl sulfate polyacrylamide gels 53,000-daltons (53-kda) and 72-kda surface antigenic proteins and a 38-kda axial flagellar protein showed the ability to bind to fibronectin, suggesting that fibronectin on host cells can mediate cytoadherence of t. denticola by its bi ... | 1993 | 8474361 |
| treponema denticola (ex brumpt 1925) sp. nov., nom. rev., and identification of new spirochete isolates from periodontal pockets. | standard growth and isolation methods were used to obtain five new treponema strains in pure culture from deep periodontal pockets. the strains were identified to the species level by various methods, including agglutination, immunofluorescence, a dot blot immunoassay, electron microscopy, gas-liquid chromatography of metabolic volatile fatty acids, and dna hybridization. two isolates were strains of treponema socranskii; the other three were strains of "treponema denticola," a species described ... | 1993 | 8494734 |
| characterization of the wheat germ agglutinin-binding property of treponema denticola. | lectins were used to characterize glycoconjugates on the cell surface of treponema denticola, a suspected periodontopathogen. bacteria were first screened by light microscopy using fluorescein isothiocyanate-coupled lectins. wheat germ agglutinin (wga) showed a high reactivity to t. denticola. while the wga-binding activity was accentuated following heating or detergent treatments of bacterial cells, the reaction was inhibited by incorporation of competing carbohydrates. scanning and transmissio ... | 1993 | 8496784 |
| antigenic behaviors of two axial flagellar proteins detected in treponema denticola. | two polypeptide antigens with molecular sizes of 34,000 daltons (34 kda) and 38 kda were separated from heated cells of a human clinical treponeme strain g7201 and treponema denticola atcc 35404, respectively. the rabbit polyclonal antisera against these antigens were produced and examined for their immunological reactions with the two heated antigens or intact spirochetal cells. immunoblot analysis showed that the 34-kda protein was also detected in t. denticola atcc 35404 and atcc 33520, and t ... | 1993 | 8502179 |
| oxygen metabolism, oxidative stress and acid-base physiology of dental plaque biofilms. | dental plaque is a natural biofilm which has been a focus of attention for many years because of its known roles in caries and periodontal diseases. acid production by plaque bacteria leads to the erosion of tooth mineral in caries, and the cariogenicity of plaque is related to population levels of acid-tolerant organisms such as mutans streptococci. however, the biofilm character of plaque allows for survival of a diverse flora, including less acid-tolerant organisms, some of which can produce ... | 1995 | 8519478 |
| a rapid method for extraction and purification of dna from dental plaque. | a rapid method based on previously described dna extraction procedures was developed for the isolation of dna from dental plaque samples. the isolated dna is suitable for use in the pcr. freeze-thawing, cell wall-degrading enzymes, and guanidine isothiocyanate were used to lyse cells and release dna. the released dna was adsorbed onto diatomaceous earth and purified by washing with guanidine isothiocyanate, ethanol, and acetone. the purified dna was released from the diatomaceous earth into an a ... | 1995 | 8526528 |
| subgingival distribution of treponema denticola, treponema socranskii, and pathogen-related oral spirochetes: prevalence and relationship to periodontal status of sampled sites. | aims of this study were to comprehensively describe the intraoral distribution of the spirochete morphogroup and of 7 antigenically distinct oral treponema, and to relate their presence to periodontal status. periodontal tissues were evaluated at 4 sites on every tooth except third molars and 76 subjects were classified according to the worst periodontal condition at any one site: group 1, gingivitis (n = 13); group 2, early periodontitis (n = 38); and group 3, advanced periodontitis (n = 25). s ... | 1995 | 8537864 |
| population genetic analysis of oral treponemes by multilocus enzyme electrophoresis. | seventeen treponemes recently isolated from necrotic pulps, periodontal and periapical infections and 17 previously well characterized oral treponemal strains were analyzed by multilocus enzyme electrophoresis. ten genetic loci were characterized on the basis of the electrophoretic mobilities of their enzymatic products. all loci were polymorphic. the average number of alleles per locus was 7.8. the genetic diversity among the electrophoretic types at each locus ranged from 0.624 to 0.836 with a ... | 1995 | 8596667 |