| [isolation of the bacterial strain of treponema pallidum with altered penicillin sensitivity]. | | 1977 | 331404 |
| [syphilis and blood transfusion]. | | 1977 | 331434 |
| [comparative ultrastructure study of treponema pallidum from rabbit and human syphilids]. | | 1977 | 331739 |
| development of of macrophage migration inhibition in rabbits infected with virulent treponema pallidum. | peritoneal exudate cells from rabbits infected with treponema pallidum nichols were used as indicators of macrophage migration inhibitory factor activity. between 3 and 15 weeks after infection, the migration of peritoneal exudate cells was inhibited in the presence of 3 to 25 microgram of t. phagedenis biovar reiter protein per ml. before this period, the migration patterns of peritoneal exudate cells from infected animals were uninhibited and similar to those from noninfected control rabbits. ... | 1977 | 332632 |
| prolonged survival of virulent treponema pallidum (nichols strain) in cell-free and tissue culture systems. | survival of treponema pallidum was found to be prolonged in the presence of tissue culture. of the 12 cultures studied, cottontail rabbit epithelium (sf1ep) supported t. pallidum for the longest time. in horizontal leighton tubes with reduced medium and an atmosphere of 5% co2 in n2, the 50% survival time (st50) was 5 to 6 days for treponemes associated with monolayers of sf1ep cells. comparable cell-free tubes had st50 values of less than 4 days. in vertical leighton tubes containing 6 ml of pr ... | 1977 | 332639 |
| demonstration by labeled treponemal antigen of specific antibodies in the tissue infiltrates of secondary syphilis. | specific antitreponemal antibodies have been demonstrated by immunofluorescence techniques in the lymphoplasmocytic infiltrates which characterize early symphilitic lesions. a purified suspension of nichols strain treponema pallidum was sonified and labeled with fluorescein isothiocyanate and applied to cryostat sections of 12 biopsy specimens from the cutaneous lesions of 11 patients with proven secondary syphilis, using a modified direct immunofluorescence procedure. specimens from various inf ... | 1977 | 333032 |
| [lupus erythematosus disseminatus (led) and positive reactions in lipid serological analysis for syphilis]. | | 1977 | 333682 |
| the spirochetes. | | 1977 | 334048 |
| [clinical evaluation of amoxicillin (ampc) treatment in syphilis (author's transl)]. | | 1977 | 335106 |
| [on the activity of amoxicillin against experimental treponema infection (author's transl)]. | | 1977 | 335107 |
| a study of swine dysentery by immunofluorescence and histology. | twenty-six specific-pathogen-free pigs were fed pure cultures of treponema hyodysenteriae. five untreated pigs were controls. distribution of this large spirochete in pigs with swine dysentery was shown by the indirect fluorescent antibody technique. findings by this method were compared with those from dark-field examination of colonic mucosal scrapings and from tissue sections. the cultures caused mucohemorrhagic colitis which by 10 days after inoculation was indistinguishable from the colitis ... | 1977 | 335630 |
| diagnosis of swine dysentery using an absorbed fluorescent antiserum. | the specifity of a direct fluorescent antibody test for the detection of pathogenic treponema hyodysenteriae associated with swine dysentery was improved by absorbing the original conjugated serum with a non pathogenic treponeme. | 1977 | 335634 |
| [the tpha test and the vdrl test as syphilis screening reactions in pregnant women and blood donors]. | | 1977 | 335668 |
| comparison of rpr 'teardrop' card test, vdrl and fta-abs tests results on sera from persons with suspected yaws in columbia. | a small study comparing results of the rapid plasma reagin (rpr) teardrop card test performed in the field, with results of venereal disease research laboratory (vdrl) and fluorescent treponemal antibody absorption (fta-abs) tests performed in the laboratory on venous blood specimens from the same suspected yaws patients was undertaken in columbia in july 1975. the results suggest that the rpr teardrop card test may be used to screen for infectious, or potentially infectious, yaws patients under ... | 1977 | 336143 |
| neurosyphilis. | in patients with abnormal neuropsychiatric symptoms and a reactive fluorescent treponemal antibody absoption (fta-abs) test in the cerebrospinal fluid (csf) or serum, a normal csf cell count and total protein concentration does not exlude late syphilis involving the central nervous system. in these patients, the presence of plasma cells in the csf cytogram, increased concentration of csf immunoglobulin g (igg), immunoelectrophoretic abnormalities in the precipitates of the igg and of the fab fra ... | 1977 | 336144 |
| characterization of the attachment of treponema pallidum (nichols strain) to cultured mammalian cells and the potential relationship of attachment to pathogenicity. | the interaction of treponema pallidum (nichols strain) with 19 different cultured mammalian cell types was examined. these types included cells derived from testis, kidney, spleen, lung, epidermis, cervix, urethra, and nerve tissue of human, rabbit, or rat origins. they represented normal and malignant cells, epithelial and fibroblastic morphology, cell lines, and cell strains, large numbers of organisms attached to the cultured cells; this attachment prolonged the time of retention of active tr ... | 1977 | 336548 |
| [comparative study of the if-abs test in syphilis with native and lyophilized ingredients]. | | 1977 | 337720 |
| hepatitis syphilitica. a clinico-pathological study of 25 cases. | specific histological alterations have been observed in 23 of the 25 patients with early syphilis with laboratory findings suggesting liver damage. treponema has been detected in the areas of liver lesion in 70 per cent of the patients. control examination carried out after penicillin therapy showed regression of the alterations. on the basis of the findings authors assert that treponema might induce hepatitis in early syphilis differing from liver damage of other origin. | 1977 | 337744 |
| in vitro cell response of treponema pallidum-infected rabbits. i. lymphocyte transformation. | lymphocyte response to phytohaemagglutinin (pha), treponema pallidum, cultivable reiter strain and cardiolipin reagent were examined using peripheral lymphocytes of t. pallidum-infected rabbits and control animals injected intratesticularly with saline extract of normal rabbit testes mixed with t. pallidum-free saline extract of infected testes. the response to pha was significantly suppressed only in the infected animals, reaching the lowest level at 30 days after infection. a substantial incre ... | 1977 | 338221 |
| selective in vitro response of thymus-derived lymphocytes from treponema pallidum-infected rabbits. | the blastogenic response of nylon wool-separated peripheral-blood lymphocytes from treponema pallidum-infected rabbits was tested in vitro with mitogens and t. pallidum antigens. the mitogenic response of the enriched t-cell population to concanavalin a and phytohemagglutinin was depressed during the first 3 to 4 weeks of infection, similar to the pattern observed with unfractionated cells. shortly thereafter, levels of blastogenesis returned to values of uninfected cultures. enhanced blast tran ... | 1977 | 338485 |
| anabolic potential of virulent treponema pallidum. | acrylamide gel autoradiography of 3h-labeled proteins from treponema pallidum demonstrates that virulent treponemes incubated in vitro synthesize a spectrum of high-molecular-weight proteins. a comparison of the protein profiles of t. pallidum with the reiter treponeme shows that t. pallidum possesses significant anabolic competence. | 1977 | 338492 |
| host response to treponema pallidum infection. ii. rabbit leukocyte migration inhibition in the presence of homologous organ extracts. | peripheral leukocytes of 67 rabbits infected intratesticularly with treponema pallidum, nichols' strain for various lengths of time were examined by the migration inhibition test for their response to extracts of normal rabbit heart, skin, brain and t. pallidum antigen. a control group of 14 animals injected intratesticularly with extract of normal rabbit tests was similarly examined for the leukocyte response to the same antigens except the brain extract. the percentage of infected animals resp ... | 1977 | 338508 |
| genetics of treponema: relationship between treponema pallidum and five cultivable treponemes. | three genetically distinct groups of treponemes have been identified by saturation reassociation assays using 125i-labeled treponemal dnas. the three groups are (i) virulent treponema pallidum (nichols strain), (ii) t. phagedenis and its biotypes reiter and kazan 5, and (iii) t. refringens biotypes nichols and noguchi. there is no detectable dna sequence homology (less than 5%) among the three groups. the groups have distinct guanine + cytosine contents: 52.4 to 53.7% for t. pallidum, 41.5% for ... | 1978 | 338577 |
| [specificity of ptha reaction in pregnancy]. | | 1977 | 339285 |
| [tpha reaction in early experimental infection in rabbits]. | | 1977 | 339286 |
| the role of a vaccine for syphilis. | | 1977 | 339377 |
| host response to treponema pallidum infection. iii. demonstration of autoantibodies to heart in sera from infected rabbits. | sera from rabbits infected intratesticularly with treponema pallidum but not from animals injected intratesticularly with other bacteria or with extract of normal rabbit testes demonstrated autoantibody to heart tissue. the antibody was organ-specific with cross-reactivity to skeletal muscle but was not species-specific. it could not be absorbed by t. pallidum, t. reiteri, veneral disease research laboratory reagent or rabbit mitochondrial preparation. the antibody had a transitional pattern of ... | 1978 | 340389 |
| cultivation of treponema pallidum. | | 1977 | 342067 |
| the in vitro cultivation of treponema pallidum. corroborative studies. | | 1977 | 342068 |
| cell-mediated immunological reactivity in neonates and infants with congenital syphilis. | thirty-eight mothers and babies were assessed by the lymphocyte transformation test for their cell-mediated immune responses to treponema pallidum and to purified protein derivative of tuberculin (ppd). all babies, including controls, appeared to be reactive when results of [3h]-thymidine incorporation were expressed as net counts per minute. this was because of high background activity, which was most marked in cells from neonates. when results were expressed as stimulation ratios, it was revea ... | 1977 | 342155 |
| [protozoa of trichomonadidae family in suspension of treponema pallidum passaged in rabbit testis]. | | 1977 | 343396 |
| tests for treponemal antibody in csf. | we studied the potential usefulness of csf treponemal tests in the diagnosis of neurosyphillis. the csf was tested with the microhemagglutination test for treponema pallidum (csf-mha-tp test) and with the csf-fta test by using undiluted csf and csf diluted in saline and in sorbent. in a prospective evaluation, of 177 nonsyphilitics, none had reactive csf-mha-tp tests and only one had a reactive csf-fta test. however, five of 15 syphilitics with no other evidence of neurosyphilis had reactive csf ... | 1978 | 343742 |
| detection of syphilitic hearing loss. | syphilis can cause sensorineural hearing loss that is potentially treatable. to determine the incidence of positive serological findings in patients with sensorineural hearing loss, the rapid plasma reagin card test (rprct) and the fta-abs test were performed in a study group composed of patients with sensorineural hearing loss of obscure origin. of the 306 patients with sensorineural hearing loss, 20 (6.5%) demonstrated a positive fta-abs as compared with two of 100 (2%) of the control group. c ... | 1978 | 343773 |
| unsustained multiplication of treponema pallidum (nichols virulent strain) in vitro in the presence of oxygen. | treponema pallidum (nichols virulent strain) was incubated with or without oxygen using a modified medium supplemented with reduced glutathione and a variety of nutrients (prnf10-b). two- to fourfold increases in treponemal numbers were observed in cultures without mammalian cells within 96 h of incubation under 5 to 6% oxygen. treponemal motility and multiplication were maintained more satisfactorily in cultures that were diluted and transferred daily, using an equal volume of fresh medium. tre ... | 1978 | 344209 |
| the treponema pallidum haemagglutination test as a screening procedure in the diagnosis of syphilis. | | 1977 | 344269 |
| false-positive fta-abs in hydralazine-induced lupus. | | 1978 | 344913 |
| [tpha reaction in cerebrospinal fluid in late syphilis]. | | 1977 | 345363 |
| [usefulness of the tpha-test as a search-reaction in the current serology of lues]. | | 1978 | 345644 |
| effects of fatty acids on motility retention by treponema pallidum in vitro. | treponema pallidum (nichols virulent strain) was incubated under 75% n2 + 20% h2 + 5% co2 in prereduced serum-free modified eagle-richter medium supplemented with different concentrations of various long-chain fatty acids complexed with fatty acid-free bovine serum albumin. motility retention was greater in medium with oleic acid containing 15 rather than 2 mg of albumin per ml. palmitic, stearic, oleic, or linoleic acid alone caused rapid loss of motility at concentrations as low as 5 microgram ... | 1978 | 346485 |
| ribosomal ribonucleic acid synthesis by virulent treponema pallidum. | ribosomal ribonucleic acid (rrna) synthesis by virulent treponema pallidum was monitored by incorporation of [(3)h]uridine into trichloroacetic acid-precipitable counts and examination of radiolabeled rrna on polyacrylamide gels. verification that rrna synthesis originated with t. pallidum was based upon co-electrophoresis with escherichia coli rrna, proportionate reductions in the amount of rrna synthesized when numbers of treponemes were decreased, and inclusion of appropriate animal cell cont ... | 1978 | 346486 |
| [immunity in syphilis]. | following a brief review of the characteristics of the causative organism of syphilis, its maintenance in laboratory animals and its relationship to the treponemes of the tropical treponematoses, consideration is given to the immune reactions and the development of antibodies to the treponeme of syphilis. the individual tests used in the diagnosis of syphilis, especially the complement fixation reactions and the flocculation tests, as well as the reactions employing specific antigens, such as th ... | 1978 | 346531 |
| [significance of treponema-specific cerebrospinal fluid antibodies for diagnosis and treatment of neurosyphilis (author's transl)]. | | 1978 | 347318 |
| in vitro activity of cefuroxime against treponema pallidum. | | 1978 | 348667 |
| microtubules in prokaryotes. | longitudinally aligned microtubules, about 220 a in diameter, have been seen in the protoplasmic cylinders of the following spirochetes (symbiotic in the hindguts of dry-wood and subterranean termites): pillotina sp., diplocalyx sp., hollandina sp. they are also present in a gliding bacterium from pterotermes occidentis. these microtubules are probably composed of tubulin, as determined by staining with fluorescent antibodies to tubulin and comigration with authentic tubulin on acrylamide gels. ... | 1978 | 349692 |
| congenital syphilis of the eye with lens involvement. | pathological examination of a girl with congenital syphilis revealed a focal granulomatous reaction involving the anterior uvea and lens. krajian's (silver) stain exhibited spiral forms that were morphologically compatible with treponema pallidum within the lens cortex. these findings have been rarely reported in early congenital lues. | 1978 | 350202 |
| cell-mediated immunity during syphilis. | | 1978 | 350348 |
| tpha test. experience at the clinic of dermatology, university of milan. | the results of serological investigations with the treponemal haemagglutination (tpha) test are reported in a large number of luetic and non-luetic patients. from these the following conclusions can be drawn: (1) the sensitivity of the tpha test is decreased if the test is carried out with micromethods; however, in our opinion, the same degree of sensitivity can be obtained either with the macromethod at dilution 1/80-1/60 or with micromethods at 1/20-1/40. (2) the sensitivity of this test is hi ... | 1978 | 350349 |
| altered immune responsiveness associated with experimental syphilis in the rabbit: elevated igm and depressed igg responses to sheep erythrocytes. | generalized suppression of immunoglobulin g (igg) synthesis detectable by depressed responses to heterologous antigens may be a mechanism by which certain parasites evade host defenses and establish chronic infections. to determine if such a mechanism occurs in syphilis, rabbits were infected with treponema pallidum, and at weekly intervals thereafter these rabbits and uninfected controls were sensitized with srbc. seven days later the number of antibody-forming cells present in the spleen was d ... | 1978 | 351056 |
| [electroimmunodiffusion reaction in the diagnosis of syphilis (author's transl)]. | a group of 800 human sera, obtained from syphilitic and nonsyphilitic individuals were tested in the counterimmunoelectrophoresis (cie) technique with reiter protein antigen. the sera were assayed for comparison in the following treponemal and nontreponemal tests: standard serologic tests for syphilis with cardiolipin; complement fixation (kolmer 1/5 vol) with reiter protein antigen (rpcf); t. pallidium immobilization (tpi) test; haemoagglutination test for t. pallidum antibodies (tpha). the sen ... | 1977 | 351696 |
| [spirochaetal hemagglutination test (tpha) with reagents of wroclaw production]. | | 1978 | 351719 |
| laboratory support in the management of syphilis. | recent developments in syphilis serology are set down. the approach today is more rational as it has some basis in immunological understandings. in spite of improvements, syphilis serology continues to have limitations, and differentiating active from inactive cases remains taxing. in primary and secondary syphilis, dark-ground microscopy is still the quickest and surest way to make the diagnosis. in latent syphilis and in late and congenital cases, as well as in instances of other treponemal in ... | 1978 | 353453 |
| [the passive hemagglutination test in the diagnosis of syphilis]. | studies were carried out on the comparative value of passive haemaglutination, cardiolipin and treponema tests in a total number of 4327 sera syphilitic patients in various stages of the disease and apparently normal subjects or free of syphilis. the results demonstrated the specificity and sensitivity of passive haemagglutination in all the stages of syphilis. in view of the qualities and simplicity of the test, the author recommends it as a screening test combined with a micromethod using the ... | 1978 | 353952 |
| [value and limitations of the methods of serological diagnosis of syphilis]. | | 1978 | 353953 |
| [extraction of the soluble protein fraction from treponema reiter for use as antigen in syphilis serodiagnosis]. | the present paper reports on two methods for extraction of the protein antigen from treponema reiter and shows the chemical and immunochemical characteristics of the extracts. serologic testing of 1810 sera from syphilitic patients in various stages of the disease, from healthy subjects and from subjects with a false positive reaction to lipoid antigens, showed the value of the reiter antigen to be equal to the cardiolipin antigens and those prepared from pathogenic treponema. it is recommended ... | 1978 | 353957 |
| attachment of virulent treponema pallidum to human mononuclear phagocytes. | the predominant phagocyte in established human syphilitic lesions is the mononuclear phagocyte. as these leucocytes may be important in immunity to treponema pallidum, the initial interaction between human phagocytes and pathogenic t. pallidum (nichols strain) was studied in vitro. motile, virulent t. pallidum attach to the surface membrane of phagocytes but are not ingested by these cells. heated, non-motile treponemes are not cell-associated but are observed free in the extracellular medium. a ... | 1978 | 354749 |
| early malignant syphilis observed during infection and reinfection in the same patient. | malignant syphilitic infections occurring in the same woman at an interval of 38 months is reported. the first infection was diagnosed as secondary papulopustular and ecthymatous syphilis. the reinfection was more severe, taking the form of secondary ulcerative syphilis with fever, general deterioration, and loss of weight. numerous treponema pallidum were present in the serum expressed from the papulopustular lesions during the first infection, but they were absent from the secretion expressed ... | 1978 | 354751 |
| evaluation of reagin card tests for syphilis. | the macroscopic rapid plasma reagin (rpr) and reagin screen (rst) card tests for syphilis were compared. test specimens were also examined with the venereal disease research laboratory (vdrl) microscopic slide test; those reactive in the card or slide tests were examined with the fluorescent treponemal antibody-absorption (fta-abs) procedure. agreement between the card tests with 2,300 specimens was 99%; among all three reagin tests it was 96.3%. with 348 specimens reactive in one or more of the ... | 1978 | 355209 |
| detection by direct immunofluorescence of antibodies to treponema pallidum in the cutaneous infiltrates of rabbit syphilomas. | by direct immunofluorescence, with use of fluorescein-labeled sonified treponema pallidum, specific antibodies were detected in the tissue infiltrates of cutaneous syphilomas in rabbits. specimens from cutaneous and mucosal inflammatory lesions induced by intradermal injection of a keratinous substance from an epidermal cyst served as controls. granular fluorescence was detected in the dermis of 11 of the 12 syphiloma specimens and corresponded to areas of heavy plasma cell infiltrates, and some ... | 1978 | 355581 |
| [passive haemagglutination test in serodiagnosis of syphilis. survey of a one-year trial in a venereal diseases prophylactic center (author's transl)]. | we report here a one-year study of the reliability of the treponema pallidum haemagglutination test (tpha) in the serological diagnosis of syphilis. by comparing tpha data with the diagnosis based on a set of grouped criteria, we were able to determine tpha accuracy. all studies were conducted at the antivenereal prophylactic center, in nice, france, on 1,775 sera. the grouped data consisted of:--serological test for syphilis (sts) using cardiolipin antigen,--medical and epidemiological records; ... | 1978 | 356704 |
| [practical value of the tpha test as a method of routine prophylactic studies]. | | 1978 | 358300 |
| [detection of antigen in patients with syphilis by means of the rabbit immune serum]. | | 1978 | 358650 |
| hemagglutination tests for syphilis antibody. | in a study of serodiagnosis of syphilis, the authors compared the specificities and sensitivities of two hemagglutination tests, a sheep-erythrocyte test (mha-tp) and a trukey-erythrocyte test (tpha), with those of the fluorescent treponemal antibody-absorption (fta-abs) test. in tests of sera from 935 patients without syphilis, the mha-tp, tpha, and fta-abs tests were reactive for 0.96, 0, and 1.3% respectively. the false-positive results were usually transient and not associated with underlyin ... | 1978 | 358824 |
| [experience gained with the treponema-pallidum haemagglutination test (tpha) in comparison to other serological methods (author's transl)]. | | 1978 | 359381 |
| [influence of different components of stored blood on the infectivity of treponema pallidum (nichols strain) (author's transl)]. | a factor with killing effect at 35 degrees c on treponema pallidum is shown to be in normal human serum. the activity of this factor is increased by the stabiliser of stored blood.--in the presence of the stabiliser t. pallidum loses its infectivity in human serum at 4 degrees c within 10 hours. the mechanism of the reaction is explained.--finally it is discussed whether these observations may be helpful in the prevention of transfusion syphilis. | 1978 | 359455 |
| counterimmunoelectrophoresis of reiter treponeme axial filaments as a diagnostic test for syphilis. | purified axial filaments from the cultivable reiter treponeme, previously shown to share an antigenic component with pathogenic treponemia pallidum, were evaluated as antigen in a diagnostic test for syphilis. antibody to the filaments was revealed by counterimmunoelectrophoresis. conditions that produced optimal results in the test were established. a total of 343 sera from normal individuals, biological false-positive reactors, and from patients in the different stages of syphilis were subject ... | 1978 | 359586 |
| [attempts at obtaining a culture of the pathogenic treponema pallidum]. | | 1978 | 360668 |
| [laboratory diagnosis of neurosyphilis]. | | 1978 | 360783 |
| [importance of direct study of treponema in primary lesions]. | | 1978 | 360784 |
| motility and chemotaxis of spirochetes. | | 1978 | 360979 |
| hepatic ultrastructure in congenital syphilis. | the ultrastructural changes in the liver in a case of congenital syphilis were studied. treponema organisms were easily demonstrated in 1-micrometer sections stained by toluidine blue, as well as in electron micrographs. most organisms were extracellular, but some were identified within hepatocytes. | 1978 | 361017 |
| specificity of the fta-abs and tpha tests during pregnancy. | in order to determine whether pregnancy influences the specificity of the fluorescent treponemal antibody absorption (fta-abs) and treponema palidum haemagglutination assay (tpha) tests, these tests, together with the quantitative fluorescent treponemal antibody (fta) and venereal disease research laboratory (vdrl) tests, were carried out simultaneously on 2000 pregnant women who attended for compulsory prenatal screening. in only one patient (0.05% of the total investigated) was a positive resu ... | 1978 | 361166 |
| [practical significance of syphilis serodiagnosis]. | recent developments in syphilis serology are set down. the new specific tests for treponema pallidum are described. reactivity in different stages of the disease including congenital and neurosyphilis is discussed. an outline is given of the possibilities of the igm-fta-abs and 19 s igm-fta-abs tests. syphilis serology today requires only 3 tests: a screening procedure, where the specific amha-tp is the method of choice, fta-abs test should be used for confirmation, and the quantitative vdrl in ... | 1978 | 361526 |
| microassay for immunoglobulin g antibodies to treponema pallidum with radioiodinated protein a from staphylococcus aureus: immunoglobulin g response in experimental syphilis in rabbits. | radioiodinated staphylococcal protein a (spa) was used to detect the immunoglobulin g (igg) antibody response to treponema pallidum in experimental syphilis. this solid-phase assay is based on the principle that spa binds avidly to the fc portion of mammalian igg. the optimal number of organisms for detection of antibody was 10(5) per microwell. of eight fixatives, 10% ethanol gave an optimum immune binding ratio of infected to normal rabbit serus at a 1:100 serum dilution. kinetic studies demon ... | 1978 | 361561 |
| demonstration of the in vitro phagocytosis of treponema pallidum by rabbit peritoneal macrophages. | evidence has been provided for the in vitro phagocytosis of virulent treponema pallidum by stimulant-induced peritoneal macrophages. after the 4-hr incubation of macrophages with t. pallidum, treponemal antigens associated with the macrophages are specifically stained using indirect immunofluorescent techniques. phagocytized treponemes appear within the cytoplasm of macrophages as round, brightly fluorescent "bodies" observable in increasing numbers as the duration of the treponeme-phagocyte int ... | 1978 | 361893 |
| classic pages in obstetrics and gynecology. vorläufiger bericht über das vorkommen von spirochaeten in syphilitischen krankheitsprodukten und bei papillomen. fritz richard schaudinn and erich hoffmann. arbeiten aus dem kaiserlichen gesundheitsamtes (berlin), vol. 22, pp. 527-534, 1905. | | 1978 | 362923 |
| heated versus unheated sera in a microhemagglutination assay for antibodies to treponema pallidum. | the effect of prior heating of sera on the results of the microhemagglutination assay for antibodies to treponema pallidum was evaluated. findings indicate that heating of sera had no significant effect on the results. | 1978 | 363746 |
| bell's palsy and secondary syphilis: csf spirochetes detected by immunofluorescence. | although bell's palsy is usually idiopathic, occasional cases may have an identifiable infectious cause. when facial paralysis results from syphilis, it usually develops during the tertiary meningovascular stage. we report a 30-year-old man with secondary syphilis who developed facial paralysis associated with acute syphilitic meningitis. spirochetes were identified in the cerebrospinal fluid by immunofluorescence using standard reagents from the fluorescent treponemal antibody absorption (fta-a ... | 1978 | 365078 |
| rapid test for diagnosis of syphilis by means of the surface fixation method using as antigens vdrl and treponema pallidum (strain nichols). | an attempt to use the "surface fixation" method for the detection of antigen-antibody reactions with vdrl and treponema pallidum has given results which corroborate conventional serological findings in patients suspected of being infected with this organism. the test is performed over strips of filter paper in which mixtures of the serum with the antigen are deposited and submitted to a special procedure of staining in order to detect antigen-antibody reactions. | 1978 | 365114 |
| radiolabeling of treponema pallidum (nichols virulent strain) in vitro with precursors for protein and rna biosynthesis. | we observed uptake of [u-14c]serine, u-14c-labeled amino acid hydrolysates, and [2-14c]uracil by virulent treponema pallidum in vitro for at least 96 h. no uptake of [2-14c]thymine, [1-14c]pyruvate, [u-14c]pyruvate, and [2-14c]uridine was detected. treponemal protein and rna biosynthetic activity was identified by erythromycin inhibition of amino acid and uracil uptake. radioactivity due to uptake of radiolabeled amino acids by residual testicular cells in the cultures remained at background lev ... | 1978 | 365745 |
| influence of oxygen tension, sulfhydryl compounds, and serum on the motility and virulence of treponema pallidum (nichols strain) in a cell-free system. | the motility and virulence of treponema pallidum (nichols strain) were monitored during incubation in a modified tissue culture medium to study the effects of oxygen tension and medium composition on survival of the organism. a basal medium of eagle minimal essential medium with 50% fresh, heat-inactivated normal rabbit serum was used inasmuch as better survival occurred with 50% normal rabbit serum than with lower concentrations. addition of 0.5 to 2.0 mm dithiothreitol or 2.0 mm dithioerythrit ... | 1978 | 365765 |
| genetics of treponema: characterization of treponema hyodysenteriae and its relationship to treponema pallidum. | saturation reassociation assays with 125i-labeled treponemal dnas show that treponema hyodysenteriae is genetically unrelated to t. pallidum (nichols), t. phagedenis biotype reiter, and t. refringens biotype noguchi. pathogenic and nonpathogenic isolates of t. hyodysenteriae exhibited 28% sequence homology and had an extremely low guanine-plus-cytosine content (25.8%). | 1978 | 365767 |
| [syphilis (author's transl)]. | | 1979 | 366599 |
| [use of dry treponema antigen in the immunofluorescence reaction in syphilis]. | | 1978 | 366946 |
| [jarisch-herxheimer's reaction as a manifestation of specific antigen-antibody reactions]. | | 1978 | 366949 |
| treponema pallidum immune adherence test for serodiagnosis of syphilis. 1: an improved method of the tpia test. | clinical investigations using the treponema pallidum immune adherence (tpia) test as reported by nelson (1953) were performed only by olansky et al in 1954. in this paper an improved method of the tpia test is described. the test was simple to perform and was highly specific for antitreponemal antibody. furthermore, as it was possible to use heated t. pallidum as antigen the antigen suspension could be preserved for long periods. the tpia test for syphilis was evaluated by examining the sera fro ... | 1978 | 367523 |
| treponema pallidum immune adherence test for serodiagnosis of syphilis. 2: comparison with glass plate, tpha, and fta-abs tests. | using sera from 340 patients with syphilis the treponema pallidum immune adherence (tpia) test was compared with the glass plate, t. pallidum haemagglutination (tpha), and fluorescent treponemal antibody-absorbed (fta-abs) tests. the results of the tpia test agreed with those of the glass plate, tpha, and fta-abs tests in 65%, 82%, and 73% of cases respectively. in the quantitative tpia test no significant correlation with the other tests was observed, and it is, therefore, concluded that the tp ... | 1978 | 367524 |
| treponema pallidum immune adherence test for serodiagnosis of syphilis. 3: clinical significance and evaluation of treatment. | antibody titres were measured in patients with clinical syphilis, and the effect of treatment on the results of the treponema pallidum immune adherence (tpia) test is reported. in the treponema pallidum haemagglutination (tpha) test little change in antibody titre occurred after treatment while in the fluorescent treponemal antibody-absorbed (fta-abs) test only a slight decrease occurred. the decrease in the antibody titre in the tpia test was similar to that in the glass plate test, but the fin ... | 1978 | 367525 |
| [the serological diagnosis of syphilis (author's transl)]. | | 1979 | 368757 |
| [filterability of treponema pallidum through sterilizing filters of varying pore size]. | | 1979 | 369223 |
| synovitis in secondary syphilis. clinical, light, and electron microscopic studies. | polyarthritis and tenosynovitis were seen as the presenting complaints in 7 patients with secondary syphilis. synovial needle biopsy was obtained in 3 cases and showed mild synovitis with infiltration by lymphocytes, plasma cells, and polymorphonuclear leukocytes; vascular congestion; and slightly increased synovial lining cells. in addition, electron microscopy in these synovial membranes showed many necrotic polymorphonuclear leukocytes, much interstitial debris and fibrin, and in 2 patients, ... | 1979 | 369568 |
| capacity of virulent treponema pallidum (nichols) for deoxyribonucleic acid synthesis. | treponema pallidum (nichols) was extracted from infected rabbit tissue, and cell lysates were prepared for monitoring thymidine kinase and deoxyribonucleic acid polymerase activities. no thymidine kinase could be demonstrated in preparations of t. pallidum or the cultivable t. phagedenis biotype reiter. significant levels of deoxyribonucleic acid polymerase were detected in both treponemal samples. interestingly, comparisons of polymerase activity among a spectrum of bacterial genera revealed a ... | 1979 | 370016 |
| [early congenital syphilis (7 cases observed in bangui hospital central african empire) (author's transl)]. | in an infant of good condition, skin changes, anemia, hepato-splenomegalia and more over radiological osseous changes are symptoms of great value which are to be confirmed by a serological test of the baby and its mother. treatment is very effective but it would be better to protect the foetus by an early detection and treatment of the syphilitic pregnant woman. | 1978 | 370499 |
| [sensitivity and specificity of the treponema pallidum hemagglutination test in syphilis screening]. | | 1979 | 370722 |
| problems affecting performance of the fluorescent treponemal antibody-absorption test for syphilis. | immunofluorescent staining of treponema pallidum was studied to clarify the effect of three factors on the results of the fluorescent treponemal antibody-absorption test: (i) heat inactivation of sera at 56 degrees c for 30 min before testing, (ii) use of multicircle slides, and (iii) tungsten illumination to visualize and assess unstained treponemes on reactive as well as nonreactive smears. it was found that serum inactivation before testing was not necessary for detection of immunoglobin g an ... | 1979 | 372219 |
| beaded fluorescence pattern of fta-abs test associated with malignancy. | a beaded pattern of immunofluorescence (if) occasionally occurs in routine fta-abs testing. this phenomenon strongly correlates with false-positive reactions in patients with sle. we report on this if finding in association with carcinoma of the colon. | 1979 | 373636 |
| the protective function of cell-mediated immunity in syphilis. | experimental evidence has been obtained to show that the cell-mediated response in syphilis is protective. the use of chemical immunosuppressors (cyclophosphamide, 5-fluorouracil) resulted in the abolition of the cell-mediated response measured by the macrophage migration inhibition test (mmi test), and was accompanied by an enhancement in the severity of the lesions during the course of experimental syphilis in the rabbit. successful adoptive transfer of resistance to syphilitic infection from ... | 1978 | 373686 |
| penicillin levels in blood and csf achieved by treatment of syphilis. | treponemicidal levels of penicillin in the csf were not obtained following intramuscular (im) injections of 600,000 iu of penicillin g procaine along with 2% aluminum monostearate (pam). treponemicidal levels of penicillin in the csf were achieved in 31 patients by probenecid, 500 mg/6 hr by mouth, with penicillin g, 500,000 iu by im injection, and by probenecid every six hours by mouth and im injections of penicillin g procaine once a day in two of three patients following injections of 600,000 ... | 1979 | 374763 |
| [results of modern syphilis serology in blood donors, at the same time a contribution to spontaneous healing of syphilis]. | 261 out of 336 845 blood donors have been shown to had undergone a syphilitic infection. about 52 per cent of these donors have had knowledge of their infection and of specific treatment of the disease. in about 43 per cent of the investigated cases the t. pallidum infection has had probably an inapparent course and healed without treatment, that means spontaneously. in times with an increasing number of infections by t. pallidum blood banks have to look intensively for donors which are in the f ... | 1979 | 375603 |
| effect of freezing conditions in liquid nitrogen on biological properties of treponema pallidum. | the influence of various conditions of freezing in liquid nitrogen on the motility, virulence, antigens, and immunogenicity of treponema pallidum was studied. the suspending medium, rate of freezing, kind and concentration of cryprotector, and duration of preincubation with cryoprotector were found to be critical. on the basis of the results obtained the optimal conditions of freezing of t. pallidum in liquid nitrogen were established. these are: the nelson-diesendruck medium with the addition o ... | 1979 | 375869 |
| effect of immunosuppressive agents on the course of experimental syphilis in rabbits. | rabbits infected intradermally with t. pallidum at four sites on the clipped back were treated with cyclophosphamide, 1 mg/kg body weight, twice before and every two days after inoculation for five months, or 5-fluorouracil 2.5 mg/kg body weight, according to the same regimen. humoral response, as measured repeatedly by vdrl, fta, and hemagglutination tests, was not affected by the application of the immunosuppressors. in contrast, the cell-mediated response, as determined by the macrophage migr ... | 1979 | 375870 |