| ferric iron reduction-linked growth yields of shewanella putrefaciens mr-1. | the anaerobic reduction of ferric citrate by shewanella putrefaciens mr-1 cells was inhibited markedly by p-chloromercuriphenylsulphonate, moderately by potassium cyanide, and to a small extent by 2-heptyl-4-hydroxyquinolone-n-oxide. iron reduction was accompanied by increases in total cellular protein, with values of 0.33-7.54 g cell protein produced per mol fe(iii) reduced. the growth yields were dependent upon the growth conditions of the inoculum and the initial concentration of fe(iii) citr ... | 1994 | 8157545 |
| shewanella putrefaciens abscess of the lower extremity. | | 1997 | 9399562 |
| [bacteremia caused by shewanella putrefaciens]. | | 1997 | 9410056 |
| isolation of anaerobic respiratory mutants of shewannella putrefaciens and genetic analysis of mutants deficient in anaerobic growth on fe3+. | a genetic approach was used to study (dissimilatory) ferric iron (fe3+) reduction in shewanella putrefaciens 200. chemical mutagenesis procedures and two rapid plate assays were developed to facilitate the screening of fe3+ reduction-deficient mutants. sixty-two putative fe3+ reduction-deficient mutants were identified, and each was subsequently tested for its ability to grow anaerobically on various compounds as sole terminal electron acceptors, including fe3+, nitrate (no3-), nitrite (no2-), m ... | 1994 | 8113188 |
| purification and properties of a novel cytochrome: flavocytochrome c from shewanella putrefaciens. | the major soluble cytochrome isolated from microaerobically grown cells of shewanella putrefaciens has been shown to be a novel type of flavocytochrome with fumarate reductase activity. this flavocytochrome, located in the periplasmic fraction of cell extracts, has been purified to homogeneity and shown to contain 4 mol of haem c and 1 mol of non-covalently bound fad per mol of protein. an m(r) value of 63,800 is estimated from sequence analysis assuming 4 mol of haem/mol of protein. in the pres ... | 1994 | 8093012 |
| bacterial community dynamics during start-up of a trickle-bed bioreactor degrading aromatic compounds. | this study was performed with a laboratory-scale fixed-bed bioreactor degrading a mixture of aromatic compounds (solvesso100). the starter culture for the bioreactor was prepared in a fermentor with a wastewater sample of a care painting facility as the inoculum and solvesso100 as the sole carbon source. the bacterial community dynamics in the fermentor and the bioreactor were examined by a conventional isolation procedure and in situ hybridization with fluorescently labeled rrna-targeted oligon ... | 1998 | 9501433 |
| arthritis due to shewanella putrefaciens. | | 1998 | 9502507 |
| biochemical and pathogenic properties of shewanella alga and shewanella putrefaciens. | we characterized 49 strains of shewanella spp. from clinical (n = 31) and nonhuman (n = 18) sources. most shewanella alga organisms (gilardi biovar 2; centers for disease control and prevention [cdc] biotype 2) originated from clinical material (92%), failed to produce acid from carbohydrates other than d-ribose, and were biochemically and enzymatically fairly homogeneous. in contrast, shewanella putrefaciens organisms (gilardi biovars 1 and 3; cdc biotype 1) were more often associated with nonh ... | 1998 | 9508312 |
| reclassification of shewanella putrefaciens owen's genomic group ii as shewanella baltica sp. nov. | the taxonomic relationship between several shewanella putrefaciens isolates from the baltic sea and reference strains of this species is presented in this study. results from dna-dna hybridization using a newly developed non-radioactive detection system and from 16s rrna gene sequencing demonstrated that s. putrefaciens is a heterogeneous species containing more than a single genomic group. the genomic group ii was phylogenetically, genotypically and phenotypically distant enough from the specie ... | 1998 | 9542087 |
| a periplasmic flavoprotein in wolinella succinogenes that resembles the fumarate reductase of shewanella putrefaciens. | during growth with fumarate as the terminal electron transport acceptor and either formate or sulfide as the electron donor, wolinella succinogenes induced a peri-plasmic protein (54 kda) that reacted with an antiserum raised against the periplasmic fumarate reductase (fcc) of shewanella putrefaciens. however, the periplasmic cell fraction of w. succinogenes did not catalyze fumarate reduction with viologen radicals. w. succinogenes grown with polysulfide instead of fumarate contained much less ... | 1998 | 9560424 |
| study of the microbial ecology of cold-smoked salmon during storage at 8 degrees c. | microbiological, chemical and sensory changes in cold-smoked salmon were studied during 5 weeks of vacuum storage at 8 degrees c. the aerobic 20 degrees c viable count reached its maximum level after 6 days (3 x 10(6) cfu g-1) however, the shelf-life of the product was estimated to be 2 or 3 weeks by the panellists, confirming that there is no correlation between those two factors. acid, pungent, sour and rancid odours and flavours and pasty texture were the main spoilage characteristics. trimet ... | 1998 | 9562883 |
| physiological function and regulation of flavocytochrome c3, the soluble fumarate reductase from shewanella putrefaciens ncimb 400. | shewanella putrefaciens produces a soluble flavocytochrome c under anaerobic growth conditions. this protein shares sequence similarity with the catalytic subunits of membrane-bound fumarate reductases from escherichia coli and other bacteria and the purified protein has fumarate reductase activity. it is shown here that this enzyme, flavocytochrome c3, is essential for fumarate respiration in vivo since disruption of the chromosomal fcca gene, which encodes flavocytochrome c3, leads to a specif ... | 1998 | 9579067 |
| effects of electron donor and acceptor conditions on reductive dehalogenation of tetrachloromethane by shewanella putrefaciens 200. | shewanella putrefaciens 200 is a nonfermentative bacterium that is capable of dehalogenating tetrachloromethane to chloroform and other, unidentified products under anaerobic conditions. since s. putrefaciens 200 can respire anaerobically by using a variety of terminal electron acceptors, including no3-, no2-, and fe(iii), it provides a unique opportunity to study the competitive effects of different electron acceptors on dehalogenation in a single organism. the results of batch studies showed t ... | 1995 | 7887629 |
| iron and manganese in anaerobic respiration: environmental significance, physiology, and regulation. | dissimilatory iron and/or manganese reduction is known to occur in several organisms, including anaerobic sulfur-reducing organisms such as geobacter metallireducens or desulfuromonas acetoxidans, and facultative aerobes such as shewanella putrefaciens. these bacteria couple both carbon oxidation and growth to the reduction of these metals, and inhibitor and competition experiments suggest that mn(iv) and fe(iii) are efficient electron acceptors similar to nitrate in redox abilities and capable ... | 1994 | 7826009 |
| phylogeny of dissimilatory sulfite reductases supports an early origin of sulfate respiration. | microorganisms that use sulfate as a terminal electron acceptor for anaerobic respiration play a central role in the global sulfur cycle. here, we report the results of comparative sequence analysis of dissimilatory sulfite reductase (dsr) genes from closely and distantly related sulfate-reducing organisms to infer the evolutionary history of dsr. a 1.9-kb dna region encoding most of the alpha and beta subunits of dsr could be recovered only from organisms capable of dissimilatory sulfate reduct ... | 1998 | 9603890 |
| spectroscopic and kinetic studies of the tetraheme flavocytochrome c from shewanella putrefaciens ncimb400. | electron paramagnetic resonance (epr) and magnetic circular dichroism (mcd) spectroscopic studies were carried out on the tetraheme flavocytochrome c from shewanella putrefaciens ncimb400. the epr spectrum reveals two sets of g-values--gz = 2.93, gy = 2.28, and gx = 1.51; and gz = 3.58--and the mcd spectrum shows a charge-transfer band at 1510 nm. these data combined show that all four hemes are low spin and have a nitrogenous sixth ligand. sequence comparisons with other tetraheme cytochromes, ... | 1995 | 7742319 |
| sialic acid-binding lectin with antibacterial activity from the horse mussel: further characterization and immunolocalization. | a heterogeneous sialic acid-binding lectin with affinity for bacterial lps was isolated and partially characterized from hemolymph of the horse mussel modiolus modiolus.(1) using two-dimensional electrophoresis with immobilized ph gradients, the lectin revealed three subunits with different molecular weight and isoelectric points (pi); mr14 (pi approximately 5.1 and approximately 5.5), 17.5 (pi approximately 5.5) and 20 (pi approximately 4.9) kda. the affinity purified lectin existed in its nati ... | 1998 | 9639084 |
| a highly selective pcr protocol for detecting 16s rrna genes of the genus pseudomonas (sensu stricto) in environmental samples. | pseudomonas species are plant, animal, and human pathogens; exhibit plant pathogen-suppressing properties useful in biological control; or express metabolic versatilities valued in biotechnology and bioremediation. specific detection of pseudomonas species in the environment may help us gain a more complete understanding of the ecological significance of these microorganisms. the objective of this study was to develop a pcr protocol for selective detection of pseudomonas (sensu stricto) in envir ... | 1998 | 9647828 |
| design and application of two rapid screening techniques for isolation of mn(iv) reduction-deficient mutants of shewanella putrefaciens. | chemical mutagenesis procedures and two newly developed rapid plate assays were used to identify two mn(iv) reduction-deficient (mnr) mutants of shewanella putrefaciens. all eleven members of a set of previously isolated fe(iii) reduction-deficient (fer) mutants displayed mnr-positive phenotypes on the plate assays and were also capable of anaerobic growth on mn(iv) as the sole terminal electron acceptor. | 1998 | 9647855 |
| occurrence of a furan fatty acid in marine bacteria. | a fatty acid containing a furan ring was detected in the cellular lipids of marine bacteria, shewanella putrefaciens, marinomonas comunis, enterobacter agglomerans, pseudomonas fluorescens, etc., which were isolated from the intestinal liquor of fishes. analytical data indicated that the fatty acid was 10,13-epoxy-11-methyloctadeca-10, 12-dienoic acid. therefore, we propose that furan fatty acids detected in marine fish are derived not only from marine plants but also from intestinal bacteria of ... | 1995 | 7548190 |
| sensorial and microbial effects of gaseous ozone on fresh scad (trachurus trachurus). | the bactericidal activity of gaseous ozone was investigated using a commercial ozone generator. five species of fish bacteria, pseudomonas putida, shewanella putrefaciens, brochothrix thermosphacta, enterobacter sp. and lactobacillus plantarum, were inoculated on agar surfaces and exposed to different ozonation times in a gas chamber. results showed ozone in relatively low concentrations (< 0.27 x 10(-3) g l-1) was an effective bactericide of vegetative cells of the five fish bacteria. the age o ... | 1998 | 9674134 |
| isolation of shewanella putrefaciens from a rheumatic heart disease patient with infective endocarditis. | | 1998 | 9675697 |
| design and application of rrna-targeted oligonucleotide probes for the dissimilatory iron- and manganese-reducing bacterium shewanella putrefaciens. | a 16s rrna-targeted oligonucleotide probe specific for the iron (fe3+)- and manganese (mn4+)-reducing bacterium shewanella putrefaciens was constructed and tested in both laboratory- and field-based hybridization experiments. the radioactively labeled probe was used to detect s. putrefaciens in field samples collected from the water column and sediments of oneida lake in new york and its major southern tributary, chittenango creek. s. putrefaciens was quantified by (i) hybridization of the probe ... | 1993 | 7506899 |
| localization and solubilization of the iron(iii) reductase of geobacter sulfurreducens | the iron(iii) reductase activity of geobacter sulfurreducens was determined with the electron donor nadh and the artificial electron donor horse heart cytochrome c. the highest reduction rates were obtained with fe(iii) complexed by nitrilotriacetic acid as an electron acceptor. fractionation experiments indicated that no iron(iii) reductase activity was present in the cytoplasm, that approximately one-third was found in the periplasmic fraction, and that two-thirds were associated with the memb ... | 1998 | 9726858 |
| qualitative and quantitative characterization of spoilage bacteria from packed fish. | the large cells recently suggested to be responsible for spoilage of packed cod, have been identified as photobacterium phosphoreum. the spoilage activity of these cells, of shewanella putrefaciens and of other microorganisms isolated form spoiled packed cod has been studied. both qualitative and quantitative tests were used for characterization of the microbial spoilage activity. the importance of the different groups of microorganisms was evaluated by comparison of microbial spoilage activity ... | 1995 | 7488527 |
| shewanella amazonensis sp. nov., a novel metal-reducing facultative anaerobe from amazonian shelf muds. | a new bacterial species belonging to the genus shewanella is described on the basis of phenotypic characterization and sequence analysis of its 16s rrna-encoding and gyrase b (gyrb) genes. this organism, isolated from shallow-water marine sediments derived from the amazon river delta, is a gram-negative, motile, polarly flagellated, facultatively anaerobic, rod-shaped eubacterium and has a g&c content of 51.7 mol%. strain sb2bt is exceptionally active in the anaerobic reduction of iron, manganes ... | 1998 | 9734053 |
| distribution and life strategies of two bacterial populations in a eutrophic lake | monoclonal antibodies and epifluorescence microscopy were used to determine the depth distribution of two indigenous bacterial populations in the stratified lake plusssee and characterize their life strategies. populations of comamonas acidovorans px54 showed a depth distribution with maximum abundances in the oxic epilimnion, whereas aeromonas hydrophila pu7718 showed a depth distribution with maximum abundances in the anoxic thermocline layer (metalimnion), i. e., in the water layer with the h ... | 1998 | 9758799 |
| modelling of microbial activity and prediction of shelf life for packed fresh fish. | prediction of shelf life based on growth of specific spoilage organisms (sso) in model substrates was studied. the effect of co2 on the growth kinetics for photobacterium phosphoreum and shewanella putrefaciens was quantified and modelled. results showed that microbial spoilage of packed cod stored with various concentrations of co2 was accurately predicted from the effect of co2 on p. phosphoreum grown in model substrates. the short shelf life extensions previously reported for packed cod there ... | 1995 | 7488526 |
| lipid composition and chemotaxonomy of pseudomonas putrefaciens (alteromonas putrefaciens). | the major polar lipids in cells of pseudomonas putrefaciens ncib 10472 grown on nutrient agar were phosphatidylethanolamine, phoisphatidylglycerol, a glucosyldiacylglycerol, a glucuronosyldiacylglycerol and an ornithine amide lipid. an additional phospholipid, tentatively identified as acyl phosphatidylglycerol or bis-phosphatidic acid, was a trace component of the wall lipids from broth cultures, which lacked the glycolipids and the ornithine amide lipid. the wall lipids from broth cultures of ... | 1980 | 7441198 |
| enzymatic recovery of elemental palladium by using sulfate-reducing bacteria | worldwide usage of platinum group metals is increasing, prompting new recovery technologies. resting cells of desulfovibrio desulfuricans reduced soluble pd2+ to elemental, cell-bound pd0 supported by pyruvate, formate, or h2 as the electron donor without biochemical cofactors. pd reduction was o2 insensitive, opening the way for recycling and recovery of pd under oxic conditions. | 1998 | 9797331 |
| the role of biomineralization in microbiologically influenced corrosion. | synthetic iron oxides (goethite, alpha-feo.oh; hematite, fe2o3; and ferrihydrite, fe(oh)3) were used as model compounds to simulate the mineralogy of surface films on carbon steel. dissolution of these oxides exposed to pure cultures of the metal-reducing bacterium, shewanella putrefaciens, was followed by direct atomic absorption spectroscopy measurement of ferrous iron coupled with microscopic analyses using confocal laser scanning and environmental scanning electron microscopies. during an 8- ... | 1998 | 9807800 |
| shewanella putrefaciens mtrb encodes an outer membrane protein required for fe(iii) and mn(iv) reduction. | iron and manganese oxides or oxyhydroxides are abundant transition metals, and in aquatic environments they serve as terminal electron acceptors for a large number of bacterial species. the molecular mechanisms of anaerobic metal reduction, however, are not understood. shewanella putrefaciens is a facultative anaerobe that uses fe(iii) and mn(iv) as terminal electron acceptors during anaerobic respiration. transposon mutagenesis was used to generate mutants of s. putrefaciens, and one such mutan ... | 1998 | 9829939 |
| genetic characterization and heterologous expression of brochocin-c, an antibotulinal, two-peptide bacteriocin produced by brochothrix campestris atcc 43754. | brochocin-c, produced by brochothrix campestris atcc 43754, is active against many strains of the closely related meat spoilage organism brochothrix thermosphacta and a wide range of other gram-positive bacteria, including spores of clostridium botulinum. purification of the active compound and genetic characterization of brochocin-c revealed that it is a chromosomally encoded, two-peptide nonlantibiotic bacteriocin. both peptides of brochocin-c are ribosomally synthesized as prepeptides that ar ... | 1998 | 9835559 |
| numerical taxonomy of psychrotrophic pseudomonads. | the taxonomy of 218 psychrotrophic pseudomonad strains (200 field strains from meat and 18 type and reference strains) was numerically studied by 174 biochemical and physiological tests. all strains were gram-negative rods, oxidative positive and motile by means of one or more polar flagella. the strains clustered into 15 groups, of which 9 were regarded as major clusters. the major clusters were designated as pseudomonas fragi (112 strains), p. fluorescens biotype iii (7 strains), p. fluorescen ... | 1982 | 7119735 |
| diversity of free-living and attached bacteria in offshore western mediterranean waters as depicted by analysis of genes encoding 16s rrna. | in a previous study (s. g. acinas, f. rodríguez-valera, and c. pedrós-alió, fems microbiol. ecol. 24:27-40, 1997), community fingerprinting by 16s rdna restriction analysis applied to mediterranean offshore waters showed that the free-living pelagic bacterial community was very different from the bacterial cells aggregated or attached to particles of more than about 8 micrometer. here we have studied both assemblages at three depths (5, 50, and 400 m) by cloning and sequencing the 16s rdna obtai ... | 1999 | 9925576 |
| identification of lactic acid bacteria from chili bo, a malaysian food ingredient. | ninety-two strains of lactic acid bacteria (lab) were isolated from a malaysian food ingredient, chili bo, stored for up to 25 days at 28 degreesc with no benzoic acid (product a) or with 7,000 mg of benzoic acid kg-1 (product b). the strains were divided into eight groups by traditional phenotypic tests. a total of 43 strains were selected for comparison of their sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) whole-cell protein patterns with a sds-page database of lab. iso ... | 1999 | 9925588 |
| involvement of cytochromes in the anaerobic biotransformation of tetrachloromethane by shewanella putrefaciens 200. | shewanella putrefaciens 200 is an obligate respiratory bacterium that can utilize a variety of terminal electron acceptors, e.g., no3-, no2-, fe(iii), and trimethylamine n-oxide, in the absence of o2. the bacterium catalyzed the reductive transformation of tetrachloromethane (ct) under anaerobic conditions. the only identified product was trichloromethane (cf), but cf production was not stoichiometric. no dichloromethane, chloromethane, or methane was produced. a chloride mass balance indicated ... | 1993 | 8285682 |
| nested duplex pcr to detect bordetella pertussis and bordetella parapertussis and its application in diagnosis of pertussis in nonmetropolitan southeast queensland, australia. | a duplex pcr to detect bordetella pertussis and bordetella parapertussis was developed with the insertion sequences is481 (b. pertussis) and is1001 (b. parapertussis) and evaluated with specimens from 520 consecutive patients presenting with possible pertussis. no culture-positive-pcr-negative results occurred, giving the method a sensitivity of 100%. for b. pertussis, 58 of 520 patients (11.2%) were positive by pcr compared to 17 of 520 patients positive (3.3%) by culture. for b. parapertussis, ... | 1999 | 9986820 |
| phylogeny of marine and freshwater shewanella: reclassification of shewanella putrefaciens ncimb 400 as shewanella frigidimarina. | dissimilatory fe(iii) reduction by shewanella putrefaciens and related species has generated considerable interest in biochemical characterization of the pathways for anaerobic electron transfer in this organism. two strains, mr-1 and ncimb 400, have been extensively used, and several respiratory enzymes have been isolated from each. it has become apparent that significant sequence differences exist between homologous proteins from these strains. the 16s rrna from ncimb 400 was sequenced and com ... | 1999 | 10028262 |
| purification and characterization of the 3-ketosteroid-delta 1-dehydrogenase of arthrobacter simplex produced in streptomyces lividans. | the 3-ketosteroid-delta 1-dehydrogenase (ks1dh) gene of arthrobacter simplex ifo12069 cloned in streptomyces lividans was overexpressed, resulting in production of the enzyme both extracellularly and intracellularly. the enzyme was purified by ammonium sulfate fractionation and chromatographies using deae-toyopearl, butyl-toyopearl and toyopearl hw55s from the supernatant of culture broth and cell-free extracts of s. lividans, and both preparations showed the same characteristics. the n-terminal ... | 1995 | 8586617 |
| dissimilatory reduction of fe(iii) and other electron acceptors by a thermus isolate. | a thermophilic bacterium that can use o2, no3-, fe(iii), and s0 as terminal electron acceptors for growth was isolated from groundwater sampled at a 3.2-km depth in a south african gold mine. this organism, designated sa-01, clustered most closely with members of the genus thermus, as determined by 16s rrna gene (rdna) sequence analysis. the 16s rdna sequence of sa-01 was >98% similar to that of thermus strain nmx2 a.1, which was previously isolated by other investigators from a thermal spring i ... | 1999 | 10049886 |
| microbiology of the oil fly, helaeomyia petrolei. | helaeomyia petrolei larvae isolated from the asphalt seeps of rancho la brea in los angeles, calif., were examined for microbial gut contents. standard counts on luria-bertani, macconkey, and blood agar plates indicated ca. 2 x 10(5) heterotrophic bacteria per larva. the culturable bacteria represented 15 to 20% of the total population as determined by acridine orange staining. the gut itself contained large amounts of the oil, had no observable ceca, and maintained a slightly acidic ph of 6.3 t ... | 1999 | 10103240 |
| structure of a phosphorylated polysaccharide from shewanella putrefaciens strain s29. | a phosphorylated polysaccharide was isolated from the aqueous layer of the phenol-water extract of a non-halophilic bacterium shewanella putrefaciens strain s29. the glycosyl phosphate linkage in the polysaccharide was split under mild acid conditions to give, after borohydride reduction, a phosphorylated oligosaccharide-alditol. on the basis of sugar analysis and 1h, 13c and 31p nmr spectroscopy, including 2d cosy, relayed cosy, rotating-frame noe spectroscopy (roesy), heteronuclear 13c,1h cosy ... | 1997 | 9373938 |
| primary shewanella alga septicemia in a patient on hemodialysis. | we report the first japanese case of primary septicemia with shewanella alga and also describe the bacteriological characteristics of and results of antibiotic susceptibility tests of the isolate. s. alga was repeatedly isolated, at times simultaneously with escherichia coli, from the blood of a 64-year-old female undergoing hemodialysis. the isolated organism was determined to be s. alga based on recently published identification criteria, such as hemolysis on sheep blood agar, no acid producti ... | 1999 | 10325394 |
| isd1, an insertion element from the sulfate-reducing bacterium desulfovibrio vulgaris hildenborough: structure, transposition, and distribution. | insertion element isd1, discovered when its transposition caused the insertional inactivation of an introduced sacb gene, is present in two copies in the genome of desulfovibrio vulgaris hildenborough. southern blot analysis indicated at least two insertion sites in the sacb gene. cloning and sequencing of a transposed copy of isd1 indicated a length of 1,200 bp with a pair of 44-bp imperfect inverted repeats at the ends, flanked by a direct repeat of the 4-bp target sequence. aagg and aatt were ... | 1998 | 9435062 |
| a role for the mannose-sensitive hemagglutinin in biofilm formation by vibrio cholerae el tor. | while much has been learned regarding the genetic basis of host-pathogen interactions, less is known about the molecular basis of a pathogen's survival in the environment. biofilm formation on abiotic surfaces represents a survival strategy utilized by many microbes. here it is shown that vibrio cholerae el tor does not use the virulence-associated toxin-coregulated pilus to form biofilms on borosilicate but rather uses the mannose-sensitive hemagglutinin (msha) pilus, which plays no role in pat ... | 1999 | 10348878 |
| evaluation of statens serum institut enteric medium for detection of enteric pathogens. | the efficacy of the statens serum institut (ssi) enteric medium for isolation and direct identification of enteric pathogens was evaluated. six different biochemical reactions can be read by using the ssi enteric medium, allowing direct identification of a range of enteric pathogens. all 248 gram-negative bacterial species that were tested grew on the ssi enteric medium. only 10 of 248 bacteria (4%) showed discrepant results in the biochemical reactions, and none of these were enteric pathogens. ... | 1999 | 10364603 |
| cytochrome c-dependent methacrylate reductase from geobacter sulfurreducens am-1. | geobacter sulfurreducens am-1 can use methacrylate as a terminal electron acceptor for anaerobic respiration. in this paper, we report on the purification and properties of the periplasmic methacrylate reductase, and show that the enzyme is dependent on the presence of a periplasmic cytochrome c (apparent k(m) = 0.12 microm). the methacrylate reductase was found to be composed of only one polypeptide with an apparent molecular mass of 50 kda and to contain, bound tightly but not covalently, 1 mo ... | 1999 | 10406941 |
| succinate dehydrogenase (sdh) from bradyrhizobium japonicum is closely related to mitochondrial sdh. | the sdhcdab operon, encoding succinate dehydrogenase, was cloned from the soybean symbiont bradyrhizobium japonicum. sdh from b. japonicum is phylogenetically related to sdh from mitochondria. this is the first example of a mitochondrion-like sdh functionally expressed in escherichia coli. | 1999 | 10419971 |
| cloning and nucleotide sequence of the gyrb gene of vibrio parahaemolyticus and its application in detection of this pathogen in shrimp. | because biochemical testing and 16s rrna sequence analysis have proven inadequate for the differentiation of vibrio parahaemolyticus from closely related species, we employed the gyrase b gene (gyrb) as a molecular diagnostic probe. the gyrb genes of v. parahaemolyticus and closely related vibrio alginolyticus were cloned and sequenced. oligonucleotide pcr primers were designed for the amplification of a 285-bp fragment from within gyrb specific for v. parahaemolyticus. these primers recognized ... | 1998 | 9464408 |
| in vitro study of tmao reduction by shewanella putrefaciens isolated from cod fillets packed in modified atmosphere. | a strain of shewanella putrefaciens was isolated from cod fillets in a modified atmosphere (60% co2, 30% o2, 10% n2). the effect of ph on the growth of shewanella putrefaciens and tmao reduction was studied in fish extract with citrate buffer systems at ph values from 5.8 to 6.8. the effect of the modified atmosphere (co2, o2) on the growth of s. putrefaciens and on the reduction of tmao to tma was studied using solid media from fish extract packed under variable mixtures of co2, o2 and n2. all ... | 1998 | 9602930 |
| inhibitory effects of protamines on proteolytic and adhesive activities of porphyromonas gingivalis. | protamines (salmine prepared from sperm dna of salmon and clupeine from herring sperm), which are basic peptides rich in arginine, were found to inhibit the proteolytic activity of arginine-specific cysteine protease (rc-protease) from porphyromonas gingivalis. lineweaver-burk plot analysis revealed that the protamines competitively inhibited proteolytic activity with cleavage of benzoyl-l-arginine-p-nitroanilide, a synthetic substrate of rc-protease. furthermore, the protamines were capable of ... | 1999 | 10456950 |
| occurrence of shewanella algae in danish coastal water and effects of water temperature and culture conditions on its survival. | the marine bacterium shewanella algae, which was identified as the cause of human cases of bacteremia and ear infections in denmark in the summers of 1994 and 1995, was detected in seawater only during the months (july, august, september, and october) when the water temperature was above 13 degrees c. the bacterium is a typical mesophilic organism, and model experiments were conducted to elucidate the fate of the organism under cold and nutrient-limited conditions. the culturable count of s. alg ... | 1999 | 10473392 |
| distribution of tetrahydromethanopterin-dependent enzymes in methylotrophic bacteria and phylogeny of methenyl tetrahydromethanopterin cyclohydrolases. | the methylotrophic proteobacterium methylobacterium extorquens am1 possesses tetrahydromethanopterin (h(4)mpt)-dependent enzymes, which are otherwise specific to methanogenic and sulfate-reducing archaea and which have been suggested to be involved in formaldehyde oxidation to co(2) in m. extorquens am1. the distribution of h(4)mpt-dependent enzyme activities in cell extracts of methylotrophic bacteria from 13 different genera are reported. h(4)mpt-dependent activities were detected in all of th ... | 1999 | 10482517 |
| mutational analysis of the recj exonuclease of escherichia coli: identification of phosphoesterase motifs. | the recj gene, identified in escherichia coli, encodes a mg(+2)-dependent 5'-to-3' exonuclease with high specificity for single-strand dna. genetic and biochemical experiments implicate recj exonuclease in homologous recombination, base excision, and methyl-directed mismatch repair. genes encoding proteins with strong similarities to recj have been found in every eubacterial genome sequenced to date, with the exception of mycoplasma and mycobacterium tuberculosis. multiple genes encoding protein ... | 1999 | 10498723 |
| growth of geobacter sulfurreducens with acetate in syntrophic cooperation with hydrogen-oxidizing anaerobic partners | pure cultures of geobacter sulfurreducens and other fe(iii)-reducing bacteria accumulated hydrogen to partial pressures of 5 to 70 pa with acetate, butyrate, benzoate, ethanol, lactate, or glucose as the electron donor if electron release to an acceptor was limiting. g. sulfurreducens coupled acetate oxidation with electron transfer to an anaerobic partner bacterium in the absence of ferric iron or other electron acceptors. cocultures of g. sulfurreducens and wolinella succinogenes with nitrate ... | 1998 | 9603840 |
| concurrent infection due to shewanella putrefaciens and mycobacterium marinum acquired at the beach. | infection with marine bacteria is uncommon. a patient with systemic lupus erythematosus who developed concurrent infection with shewanella putrefaciens and mycobacterium marinum (m. marinum) is described. after bathing leg ulcers in sea water, severe cellulitis of the left leg with necrotic areas and extensive bullae developed. infection due to s. putrefaciens was confirmed and a long course of hospitalization, oral ciprofloxacin and skin grafting was required. during hospitalization subcutaneou ... | 1998 | 9611378 |
| a periplasmic and extracellular c-type cytochrome of geobacter sulfurreducens acts as a ferric iron reductase and as an electron carrier to other acceptors or to partner bacteria. | an extracellular electron carrier excreted into the growth medium by cells of geobacter sulfurreducens was identified as a c-type cytochrome. the cytochrome was found to be distributed in about equal amounts in the membrane fraction, the periplasmic space, and the surrounding medium during all phases of growth with acetate plus fumarate. it was isolated from periplasmic preparations and purified to homogeneity by cation-exchange chromatography, gel filtration, and hydrophobic interaction chromat ... | 1998 | 9658015 |
| identification of the syntrophic partners in a coculture coupling anaerobic methanol oxidation to fe(iii) reduction. | from enrichments with methanol and ferric pyrophosphate a coculture was isolated which coupled methanol oxidation to carbon dioxide with the reduction of fe(iii) to fe(ii). 16s rrna gene analysis of the isolated syntrophic partners revealed 99.5% similarity to clostridium sphenoides and 98.5% to shewanella putrefaciens. formation of fe(ii) coupled to methanol oxidation was confirmed by using strains of culture collections (c. sphenoides dsm 632 and s. putrefaciens dsm 9461). the importance of th ... | 1999 | 10556712 |
| volatile components associated with bacterial spoilage of tropical prawns. | analysis of headspace volatiles by gas chromatography/mass spectrometry from king (penaeus plebejus), banana (p. merguiensis), tiger (p. esculentus/semisulcatus) and greasy (metapenaeus bennettae) prawns stored in ice or ice slurry, which is effectively an environment of low oxygen tension, indicated the presence of amines at the early stages of storage (less than 8 days) irrespective of the nature of the storage media. esters were more prevalent in prawns stored on ice (normal oxygen conditions ... | 1998 | 9706797 |
| spme-ms-mva as an electronic nose for the study of off-flavors in milk. | a new technique using solid-phase microextraction, mass spectrometry, and multivariate analysis (spme-ms-mva) was developed for the study of off-flavors in milk. the analytical column of a gc/ms system was replaced with a 1-m deactivated fused-silica column, which served as a transfer line to deliver volatiles extracted from milk samples with a carboxen-spme fiber to the mass spectrometer. mass fragmentation data resulting from the unresolved milk volatile components were subjected to mva. princ ... | 1999 | 10563947 |
| isolation and sequence of omca, a gene encoding a decaheme outer membrane cytochrome c of shewanella putrefaciens mr-1, and detection of omca homologs in other strains of s. putrefaciens. | the sequence of the omca gene, which encodes a decaheme cytochrome c that is localized to the outer membrane (om) of shewanella putrefaciens mr-1, was determined. the 2202 bp nucleotide sequence of omca encodes for 734 amino acids with a predicted molecular protein mass of 78.6 kda. comparison with the amino-terminal sequence of the mature protein suggests the presence of a hydrophobic leader sequence which is cleaved during translocation of the protein to the om. this leader sequence has a lipo ... | 1998 | 9733973 |
| use of time-temperature integrators and predictive modelling for shelf life control of chilled fish under dynamic storage conditions. | a systematic approach for fish shelf life modelling and time temperature integrator (tti) selection in order to plan and apply an effective quality monitoring scheme for the fish chill chain was developed. the temperature behaviour of the natural microflora of the mediterranean fish boque (boops boops) was studied and growth of the specific spoilage bacteria pseudomonas spp. and shewanella putrefaciens was modelled and correlated to organoleptic shelf life. arrhenius and square root functions we ... | 1999 | 10598111 |
| acetone formation in the vibrio family: a new pathway for bacterial leucine catabolism. | there is current interest in biological sources of acetone, a volatile organic compound that impacts atmospheric chemistry. here, we determined that leucine-dependent acetone formation is widespread in the vibrionaceae. sixteen vibrio isolates, two listonella species, and two photobacterium angustum isolates produced acetone in the presence of l-leucine. shewanella isolates produced much less acetone. growth of vibrio splendidus and p. angustum in a fermentor with controlled aeration revealed th ... | 1999 | 10601206 |
| role of the tetraheme cytochrome cyma in anaerobic electron transport in cells of shewanella putrefaciens mr-1 with normal levels of menaquinone. | shewanella putrefaciens mr-1 possesses a complex electron transport system which facilitates its ability to use a diverse array of compounds as terminal electron acceptors for anaerobic respiration. a previous report described a mutant strain (cmtn-1) deficient in cyma, a tetraheme cytochrome c. however, the interpretation of the electron transport role of cyma was complicated by the fact that cmtn-1 was also markedly deficient in menaquinones. this report demonstrates that the depressed menaqui ... | 2000 | 10613864 |
| homogeneity of danish environmental and clinical isolates of shewanella algae. | danish isolates of shewanella algae constituted by whole-cell protein profiling a very homogeneous group, and no clear distinction was seen between strains from the marine environment and strains of clinical origin. although variation between all strains was observed by ribotyping and random amplified polymorphic dna analysis, no clonal relationship between infective strains was found. from several patients, clonally identical strains of s. algae were reisolated up to 8 months after the primary ... | 2000 | 10618264 |
| biosynthesis of terpenoids: ychb protein of escherichia coli phosphorylates the 2-hydroxy group of 4-diphosphocytidyl-2c-methyl-d-erythritol. | a comparative analysis of all published complete genomes indicated that the putative orthologs of the unannotated ychb gene of escherichia coli follow the distribution of the dxs, dxr, and ygbp genes, which have been shown to specify enzymes of the deoxyxylulose phosphate pathway of terpenoid biosynthesis, thus suggesting that the hypothetical ychb protein also is involved in that pathway. to test this hypothesis, the e. coli ychb gene was expressed in a homologous host. the recombinant protein ... | 2000 | 10655484 |
| evolution of arginine biosynthesis in the bacterial domain: novel gene-enzyme relationships from psychrophilic moritella strains (vibrionaceae) and evolutionary significance of n-alpha-acetyl ornithinase. | in the arginine biosynthetic pathway of the vast majority of prokaryotes, the formation of ornithine is catalyzed by an enzyme transferring the acetyl group of n-alpha-acetylornithine to glutamate (ornithine acetyltransferase [oatase]) (argj encoded). only two exceptions had been reported-the enterobacteriaceae and myxococcus xanthus (members of the gamma and delta groups of the class proteobacteria, respectively)-in which ornithine is produced from n-alpha-acetylornithine by a deacylase, acetyl ... | 2000 | 10692366 |
| functionality of purified sigma(n) (sigma(54)) and a nifa-like protein from the hyperthermophile aquifex aeolicus. | the genome sequence of the extremely thermophilic bacterium aquifex aeolicus encodes alternative sigma factor sigma(n) (sigma(54), rpon) and five potential sigma(n)-dependent transcriptional activators. although a. aeolicus possesses no recognizable nitrogenase genes, two of the activators have a high degree of sequence similarity to nifa proteins from nitrogen-fixing proteobacteria. we identified five putative sigma(n)-dependent promoters upstream of operons implicated in functions including su ... | 2000 | 10692367 |
| biosynthesis of terpenoids: ygbb protein converts 4-diphosphocytidyl-2c-methyl-d-erythritol 2-phosphate to 2c-methyl-d-erythritol 2,4-cyclodiphosphate. | in many microorganisms, the putative orthologs of the escherichia coli ygbb gene are tightly linked or fused to putative orthologs of ygbp, which has been shown earlier to be involved in terpenoid biosynthesis. the ygbb gene of e. coli was expressed in a recombinant e. coli strain and was shown to direct the synthesis of a soluble, 17-kda polypeptide. the recombinant protein was found to convert 4-diphosphocytidyl-2c-methyl-d-erythritol 2-phosphate into 2c-methyl-d-erythritol 2,4-cyclodiphosphat ... | 2000 | 10694574 |
| reduction of fe(iii), mn(iv), and toxic metals at 100 degrees c by pyrobaculum islandicum. | it has recently been noted that a diversity of hyperthermophilic microorganisms have the ability to reduce fe(iii) with hydrogen as the electron donor, but the reduction of fe(iii) or other metals by these organisms has not been previously examined in detail. when pyrobaculum islandicum was grown at 100 degrees c in a medium with hydrogen as the electron donor and fe(iii)-citrate as the electron acceptor, the increase in cell numbers of p. islandicum per mole of fe(iii) reduced was found to be c ... | 2000 | 10698770 |
| manganese reduction by microbes from oxic regions of the lake vanda (antarctica) water column | depth profiles of metals in lake vanda, a permanently ice-covered, stratified antarctic lake, suggest the importance of particulate manganese oxides in the scavenging, transport, and release of metals. since manganese oxides can be solubilized by manganese-reducing bacteria, microbially mediated manganese reduction was investigated in lake vanda. microbes concentrated from oxic regions of the water column, encompassing a peak of soluble manganese [mn(ii)], reduced synthetic manganese oxides (mno ... | 1998 | 9758801 |
| identification and population structure of burkholderia stabilis sp. nov. (formerly burkholderia cepacia genomovar iv). | the burkholderia cepacia complex currently comprises five genomic species, i.e., b. cepacia genomovar i, b. multivorans (formerly known as b. cepacia genomovar ii), b. cepacia genomovar iii, b. cepacia genomovar iv, and b. vietnamiensis (also known as b. cepacia genomovar v). in the absence of straightforward diagnostic tests for the identification of b. cepacia genomovars i, iii, and iv, the last two genomic species were not formally classified as novel burkholderia species (genomovar i contain ... | 2000 | 10698993 |
| evaluation of autoscan-w/a and the vitek gni+ automicrobic system for identification of non-glucose-fermenting gram-negative bacilli. | the autoscan-w/a (w/a; dade behring microscan inc., west sacramento, calif.) and vitek automicrobic system (vitek ams; biomérieux vitek systems, inc., hazelwood, mo.) are both fully automated microbiology systems. we evaluated the accuracy of these two systems in identifying nonglucose-fermenting gram-negative bacilli. we used the w/a with conventional-panel neg combo type 12 and vitek gni+ identification systems. a total of 301 isolates from 25 different species were tested. of these, 299 isola ... | 2000 | 10699007 |
| identification of a marine agarolytic pseudoalteromonas isolate and characterization of its extracellular agarase | the phenotypic and agarolytic features of an unidentified marine bacteria that was isolated from the southern pacific coast was investigated. the strain was gram negative, obligately aerobic, and polarly flagellated. on the basis of several phenotypic characters and a phylogenetic analysis of the genes coding for the 16s rrna, this strain was identified as pseudoalteromonas antarctica strain n-1. in solid agar, this isolate produced a diffusible agarase that caused agar softening around the colo ... | 1998 | 9797294 |
| characterization of the uup locus and its role in transposon excisions and tandem repeat deletions in escherichia coli. | null mutations in the escherichia coli uup locus (at 21.8 min) serve to increase the frequency of reca-independent precise excision of transposable elements such as tn10 and to reduce the plaque size of bacteriophage mu (uup(-) phenotype). by the combined approaches of physical mapping of the mutations, complementation analyses, and protein overexpression from cloned gene fragments, we have demonstrated in this study that the uup(-) phenotype is the consequence of the absence of expression of th ... | 2000 | 10715006 |
| effect of toxic metals on indigenous soil beta-subgroup proteobacterium ammonia oxidizer community structure and protection against toxicity by inoculated metal-resistant bacteria. | contamination of soils with toxic metals is a major problem on military, industrial, and mining sites worldwide. of particular interest to the field of bioremediation is the selection of biological markers for the end point of remediation. in this microcosm study, we focus on the effect of addition of a mixture of toxic metals (cadmium, cobalt, cesium, and strontium as chlorides) to soil on the population structure and size of the ammonia oxidizers that are members of the beta subgroup of the pr ... | 1999 | 9872765 |
| chromium(vi) reductase activity is associated with the cytoplasmic membrane of anaerobically grown shewanella putrefaciens mr-1. | shewanella putrefaciens mr-1 can reduce a diverse array of compounds under anaerobic conditions, including manganese and iron oxides, fumarate, nitrate, and many other compounds. these reductive processes are apparently linked to a complex electron transport system. chromium (cr) is a toxic and mutagenic metal and bacteria could potentially be utilized to immobilize cr by reducing the soluble and bioavailable state, cr(vi), to the insoluble and less bioavailable state, cr(iii). formate-dependent ... | 2000 | 10735248 |
| peptidase e, a peptidase specific for n-terminal aspartic dipeptides, is a serine hydrolase. | salmonella enterica serovar typhimurium peptidase e (pepe) is an n-terminal asp-specific dipeptidase. pepe is not inhibited by any of the classical peptidase inhibitors, and its amino acid sequence does not place it in any of the known peptidase structural classes. a comparison of the amino acid sequence of pepe with a number of related sequences has allowed us to define the amino acid residues that are strongly conserved in this family. to ensure the validity of this comparison, we have express ... | 2000 | 10762256 |
| chemical and sensory changes associated with microbial flora of mediterranean boque (boops boops) stored aerobically at 0, 3, 7, and 10 degreesc. | the development of a microbial population and changes in the physicochemical and sensorial characteristics of mediterranean boque (boops boops), called gopa in greece, stored aerobically at 0, 3, 7, and 10 degreesc were studied. pseudomonads and shewanella putrefaciens were the dominant bacteria at the end of the storage period, regardless of the temperature tested. enterobacteria and brochothrix thermosphacta also grew, but their population density was always 2 to 3 log10 cfu g-1 less than that ... | 1999 | 9925603 |
| targeted disruption of the kstd gene encoding a 3-ketosteroid delta(1)-dehydrogenase isoenzyme of rhodococcus erythropolis strain sq1. | microbial phytosterol degradation is accompanied by the formation of steroid pathway intermediates, which are potential precursors in the synthesis of bioactive steroids. degradation of these steroid intermediates is initiated by delta(1)-dehydrogenation of the steroid ring structure. characterization of a 2.9-kb dna fragment of rhodococcus erythropolis sq1 revealed an open reading frame (kstd) showing similarity with known 3-ketosteroid delta(1)-dehydrogenase genes. heterologous expression of k ... | 2000 | 10788377 |
| bacteriology of infected cavitating lung tumor. | differentiation between in situ infection and simple tumor necrosis in cavitating lung tumors by means of imaging studies is difficult. in this study, we prospectively investigated the role of ultrasound (us)-guided transthoracic aspiration for bacteriologic examination of infected cavitating lung tumors, and the influence of the culture results on the treatment of patients. twenty-two patients (18 men and four women) with cavitating lung tumors treated from january 1996 to october 1998 were inc ... | 2000 | 10806182 |
| a membrane-bound flavocytochrome c-sulfide dehydrogenase from the purple phototrophic sulfur bacterium ectothiorhodospira vacuolata. | the amino acid sequence of ectothiorhodospira vacuolata cytochrome c-552, isolated from membranes with n-butanol, shows that it is a protein of 77 amino acid residues with a molecular mass of 9,041 da. it is closely related to the cytochrome subunit of chlorobium limicola f. sp. thiosulfatophilum flavocytochrome c-sulfide dehydrogenase (fcsd), having 49% identity. these data allowed isolation of a 5.5-kb subgenomic clone which contains the cytochrome gene and an adjacent flavoprotein gene as in ... | 2000 | 10809687 |
| inhibition of vibrio anguillarum by pseudomonas fluorescens ah2, a possible probiotic treatment of fish. | to study the possible use of probiotics in fish farming, we evaluated the in vitro and in vivo antagonism of antibacterial strain pseudomonas fluorescens strain ah2 against the fish-pathogenic bacterium vibrio anguillarum. as iron is important in virulence and bacterial interactions, the effect of p. fluorescens ah2 was studied under iron-rich and iron-limited conditions. sterile-filtered culture supernatants from iron-limited p. fluorescens ah2 inhibited the growth of v. anguillarum, whereas st ... | 1999 | 10049849 |
| production of eicosapentaenoic acid from marine bacteria. | a marine bacterium, judged as a new species close to shewanella putrefaciens, was isolated from the intestinal contents of the pacific mackerel. the isolated strain scrc-2378 produced eicosapentaenoic acid (epa) as the sole polyunsaturated fatty acid, which amounted to 24-40% of the total fatty acid in the cell, which corresponded to 2% of dry cell weight. under the optimal growth conditions (ph 7.0, 20 degrees c, and grown aerobically for 12-18 h), the yield of scrc-2738 reached 15 g of dry cel ... | 1996 | 8729138 |
| shewanella alga bacteremia in two patients with lower leg ulcers. | the first danish cases of shewanella alga bacteremia in two patients with chronic lower leg ulcers are reported. both patients were admitted to the hospital during the same month of a very warm summer and had been exposed to the same marine environment, thereby suggesting the same source of infection. both patients survived; however, one of them had extensive myonecrosis, while the other patient had an uncomplicated course. the strains were initially believed to be shewanella putrefaciens on the ... | 1996 | 8783706 |
| spoilage bacteria of fresh broiler chicken carcasses. | studies were conducted to identify the bacteria responsible for spoilage of fresh broiler chicken carcasses and to characterize the off-odors these bacteria produce. broiler carcasses were collected from processing plants in the northeast georgia area, the southeastern u.s., arkansas, california, and north carolina. the carcasses were allowed to spoil under controlled conditions at 3 c and spoilage bacteria were isolated. each spoilage bacterium was separately inoculated into a sterile chicken s ... | 1995 | 8825595 |
| purification and properties of a low-redox-potential tetraheme cytochrome c3 from shewanella putrefaciens. | shewanella putrefaciens is a facultatively anaerobic bacterium in the gamma group of the proteobacteria, capable of utilizing a wide variety of anaerobic electron acceptors. an examination of its cytochrome content revealed the presence of a tetraheme, low-redox-potential (e'o = -233 mv), cytochrome c-type cytochrome with a molecular mass of 12,120 da and a pi of 5.8. the electron spin resonance data indicate a bis-histidine coordination of heme groups. reduction of ferric citrate was accompanie ... | 1996 | 8892848 |
| polyphasic taxonomic approach in the description of alishewanella fetalis gen. nov., sp. nov., isolated from a human foetus. | a taxonomically unique bacterium is described on the basis of a physiological and biochemical characterization, fatty acid profiling and sequence analyses of 16s rrna and gyrase b (gyrb) genes. this non-motile, non-fermentative bacterium was isolated from a human foetus in uppsala, sweden, and originally misidentified as a shewanella putrefaciens by conventional biochemical testing. the bacterium grew well at mesophilic temperatures with optimum growth at 37 degrees c. it was facultatively anaer ... | 2000 | 10843055 |
| identification of a novel gene, fimv, involved in twitching motility in pseudomonas aeruginosa. | transposon mutagenesis was used to identify a new locus required for twitching motility in pseudomonas aeruginosa. four tn5-b21 mutants which lacked twitching motility and a fifth which exhibited impaired motility were found to map to the same kpn:i restriction fragment at approximately 40 min on the p. aeruginosa genome. cloning and sequencing studies showed that all five transposon insertions occurred within the same 2.8 kb orf, which was termed fimv. the product of this gene has a putative pe ... | 2000 | 10846211 |
| do stresses encountered during the smoked salmon process influence the survival of the spoiling bacterium shewanella putrefaciens? | the influence of different treatments (i.e. cold, nacl, phenol and anaerobiosis) encountered during the smoked salmon process was studied by analysing the survival capacity of two shewanella putrefaciens strains (cip 69.29 and j13.1). our results indicated that only the salt stress was critical for the survival of s. putrefaciens. nevertheless, both strains of s. putrefaciens grown at low temperatures developed a cross-protection to a lethal nacl treatment. to our knowledge, this is the first re ... | 2000 | 10849272 |
| cyanobacterial sulfide-quinone reductase: cloning and heterologous expression. | the gene encoding sulfide-quinone reductase (sqr; e.c.1.8.5.'), the enzyme catalyzing the first step of anoxygenic photosynthesis in the filamentous cyanobacterium oscillatoria limnetica, was cloned by use of amino acid sequences of tryptic peptides as well as sequences conserved in the rhodobacter capsulatus sqr and in an open reading frame found in the genome of aquifex aeolicus. sqr activity was also detected in the unicellular cyanobacterium aphanothece halophytica following sulfide inductio ... | 2000 | 10852862 |
| salmonella enterica serovar typhimurium peptidase b is a leucyl aminopeptidase with specificity for acidic amino acids. | peptidase b (pepb) of salmonella enterica serovar typhimurium is one of three broad-specificity aminopeptidases found in this organism. we have sequenced the pepb gene and found that it encodes a 427-amino-acid (46.36-kda) protein, which can be unambiguously assigned to the leucyl aminopeptidase (lap) structural family. pepb has been overexpressed and purified. the active enzyme shows many similarities to other members of the lap family: it is a heat-stable (70 degrees c; 20 min) hexameric ( app ... | 2000 | 10852868 |
| exopolysaccharide production is required for development of escherichia coli k-12 biofilm architecture. | although exopolysaccharides (epss) are a large component of bacterial biofilms, their contribution to biofilm structure and function has been examined for only a few organisms. in each of these cases eps has been shown to be required for cellular attachment to abiotic surfaces. here, we undertook a genetic approach to examine the potential role of colanic acid, an eps of escherichia coli k-12, in biofilm formation. strains either proficient or deficient in colanic acid production were grown and ... | 2000 | 10852895 |
| bacterial spoilage of meat and cured meat products. | the influence of environmental factors (product composition and storage conditions) on the selection, growth rate and metabolic activity of the bacterial flora is presented for meat (pork and beef) and cooked, cured meat products. the predominant bacteria associated with spoilage of refrigerated beef and pork, are brochothrix thermosphacta, carnobacterium spp., enterobacteriaceae, lactobacillus spp., leuconostoc spp., pseudomonas spp. and shewanella putrefaciens. the main defects in meat are off ... | 1996 | 8913812 |
| the bacterial cell-division protein zipa and its interaction with an ftsz fragment revealed by x-ray crystallography. | in escherichia coli, ftsz, a homologue of eukaryotic tubulins, and zipa, a membrane-anchored protein that binds to ftsz, are two essential components of the septal ring structure that mediates cell division. recent data indicate that zipa is involved in the assembly of the ring by linking ftsz to the cytoplasmic membrane and that the zipa-ftsz interaction is mediated by their c-terminal domains. we present the x-ray crystal structures of the c-terminal ftsz-binding domain of zipa and a complex b ... | 2000 | 10880432 |
| cloning and sequence of cyma, a gene encoding a tetraheme cytochrome c required for reduction of iron(iii), fumarate, and nitrate by shewanella putrefaciens mr-1. | the cyma gene, which encodes a tetraheme cytochrome c, was cloned from shewanella putrefaciens mr-1. this gene complemented a mutant which had a tnphoa insertion in cyma and which was deficient in the respiratory reduction of iron(iii), nitrate, fumarate, and manganese(iv). the 561-bp nucleotide sequence of cyma encodes a protein of 187 amino acids with a predicted molecular mass of 20.8 kda. no n-terminal signal sequence was readily apparent; consistent with this, a cytochrome with a size of 21 ... | 1997 | 9023196 |
| human infection with halomonas venusta following fish bite. | halomonas venusta, a moderately halophilic, nonfermentative gram-negative rod, is reported for the first time as a human pathogen in a wound that originated from a fish bite. | 2000 | 10921995 |
| molecular characterization of the beta-n-acetylglucosaminidase of escherichia coli and its role in cell wall recycling. | the beta-n-acetylglucosaminidase of escherichia coli was found to have a novel specificity and to be encoded by a gene (nagz) that maps at 25.1 min. it corresponds to an open reading frame, ycfo, whose predicted amino acid sequence is 57% identical to that of vibrio furnissii exoii. nagz hydrolyzes the beta-1,4 glycosidic bond between n-acetylglucosamine and anhydro-n-acetylmuramic acid in cell wall degradation products following their importation into the cell during the process for recycling c ... | 2000 | 10940025 |
| replication of plasmids with the p15a origin in shewanella putrefaciens mr-1. | the plasmid pacyc184 was introduced into shewanella putrefaciens mr-1 by electroporation. in 100% of the transformants examined, the plasmid was maintained as a free replicon outside the chromosome. this was the case whether or not the plasmid contained a 224-bp dna insert derived from an open-reading frame of mr-1 genomic dna. therefore, in contrast to a report in the literature, plasmids containing the p15a origin of replication can replicate freely in s. putrefaciens mr-1, and do not make con ... | 1997 | 9080705 |