| a plasma inhibitor of platelet aggregation in patients with argentine hemorrhagic fever. | hemorrhage in patients with lassa fever is associated with the presence of a circulating plasma inhibitor of platelet aggregation. this study was to determine whether patients with argentine hemorrhagic fever (ahf) develop a similar inhibitor. normal platelets showed significantly weaker aggregation responses to a sub-maximal dose of adenosine diphosphate (adp) when mixed with plasma from 10 patients with ahf (mean percent of control +/- 1 se = 57.2 +/- 6.7%) compared to those mixed with plasma ... | 1990 | 2160197 |
| junin virus-induced suppressor cells are effective only on the efferent limb of the delayed-type hypersensitivity response. | junin virus (jv), the etiological agent of argentinian hemorrhagic fever, induces high mortality in the suckling balb/c mouse, which correlates with delayed-type hypersensitivity (dth)-like immune response. in contrast, the adult mouse is resistant to infection, and no dth response can be detected. an antigen-nonspecific dth suppressor t-cell activity induced by jv has been described that may be related to adult mouse survival. in this report, we present evidence supporting the inability of such ... | 1990 | 2160514 |
| a mouse attenuated mutant of junin virus with an altered envelope glycoprotein. | the xjc13 strain of junin virus (jv) and the mouse-attenuated mutant c167 showed different gp38 peptide mapping after limited proteolysis with ficin and papain; viral infectivity of both viruses also exhibited a different susceptibility to protease treatment. a correlation between envelope glycoprotein alteration and jv virulence in neonatal mice is proposed. | 1990 | 2162164 |
| a refined complement-enhanced neutralization test for detecting antibodies to junin virus. | a refined, complement-enhanced, plaque-reduction neutralization test was developed for measuring neutralizing antibodies against junin (argentine hemorrhagic fever) virus. the assay measured neutralizing antibodies after natural as well as vaccine-induced junin virus infections. among vaccinated individuals, titers were 2-4-fold higher than those obtained with conventional assays, without loss of specificity. enhanced sensitivity was achieved by using a standardized complement source (vs human o ... | 1990 | 2170437 |
| a comparison of junin virus strains: growth characteristics, cytopathogenicity and viral polypeptides. | the growth characteristics, cytopathogenicity and viral polypeptides of the virulent strain xj of junin virus (jv), its attenuated derivative xjc13 and another naturally attenuated jv strain, iv4454, were comparatively studied. iv4454 and xjc13 viruses showed the highest and lowest cytopathology for vero cells, respectively, as measured by plaque morphology, cell viability and inhibition of host cell protein synthesis. the kinetics and electrophoretic patterns of viral polypeptides in infected c ... | 1990 | 2177565 |
| vertical transmission of junin virus in experimentally infected adult calomys musculinus. | the response to infection with junin virus, wild strain cba an 9446, and the antenatal and postnatal transmission of the pathogen in its natural host, calomys musculinus, were studied. intranasal infection in adult animals (90-120 days) did not produce mortality or illness during the 150-day period of observation. from day 21 to 150 after infection, 50% of the animals showed viral persistence with shedding of virus in both urine and saliva. the remaining half became seropositive, and no infectio ... | 1990 | 2177742 |
| vertical transmission of junin virus in experimentally infected adult calomys musculinus. | the response to infection with junin virus, wild strain cba an 9446, and the antenatal and postnatal transmission of the pathogen in its natural host, calomys musculinus, were studied. intranasal infection in adult animals (90-120 days) did not produce mortality or illness during the 150-day period of observation. from day 21 to 150 after infection, 50% of the animals showed viral persistence with shedding of virus in both urine and saliva. the remaining half became seropositive, and no infectio ... | 1990 | 2177742 |
| neurovirulence of wild and laboratory junin virus strains in animal hosts. | the neurovirulence of candid #1 and xjcl3 laboratory strains and cbalv4454 and cbafha5069 wild strains of junin virus was studied in albino mice, guinea pigs, and a south american wild rodent, calomys musculinus, of different ages inoculated by the intracerebral route. infectivity in brain and organs, lethality, and neuropathological lesions were determined. the laboratory and wild strains showed similar neurovirulence only in 2-day-old mice. the neurovirulence of laboratory strains decreased wi ... | 1990 | 2177781 |
| neurovirulence of wild and laboratory junin virus strains in animal hosts. | the neurovirulence of candid #1 and xjcl3 laboratory strains and cbalv4454 and cbafha5069 wild strains of junin virus was studied in albino mice, guinea pigs, and a south american wild rodent, calomys musculinus, of different ages inoculated by the intracerebral route. infectivity in brain and organs, lethality, and neuropathological lesions were determined. the laboratory and wild strains showed similar neurovirulence only in 2-day-old mice. the neurovirulence of laboratory strains decreased wi ... | 1990 | 2177781 |
| heterozygosity and gene flow in populations of calomys laucha (rodentia, cricetidae). | enzyme polymorphism was studied by means of starch gel electrophoresis on five population samples of calomys laucha collected in different sites and seasons. c. laucha, a cricetid rodent inhabiting preferentially cultivated fields in the central-eastern plains of argentina, is a reservoir-host of junin virus, agent of argentine hemorrhagic fever. results show high levels of genic variability in all the samples (p from 62.50 to 77.27; h from 0.118 to 0.163) and low genetic distance among populati ... | 1990 | 2194995 |
| heterozygosity and gene flow in populations of calomys laucha (rodentia, cricetidae). | enzyme polymorphism was studied by means of starch gel electrophoresis on five population samples of calomys laucha collected in different sites and seasons. c. laucha, a cricetid rodent inhabiting preferentially cultivated fields in the central-eastern plains of argentina, is a reservoir-host of junin virus, agent of argentine hemorrhagic fever. results show high levels of genic variability in all the samples (p from 62.50 to 77.27; h from 0.118 to 0.163) and low genetic distance among populati ... | 1990 | 2194995 |
| properties and characterization of monoclonal antibodies to tacaribe virus. | monoclonal antibodies prepared against tacaribe and junin viruses have been used to define further the serological relationships between arenaviruses of the tacaribe complex. a close relationship was found between these two viruses and the heterologous amapari and machupo viruses, with pichinde virus and parana virus being more distantly related. among the antibodies specific for tacaribe virus, five were found to react with viral antigens at the surface of infected cells and to neutralize virus ... | 1985 | 2410550 |
| early protection to junín virus of guinea pig with an attenuated junín virus strain. | inoculation of guinea pigs with attenuated xjo junín virus (jv) strain confers protection against challenge with pathogenic xj-jv strain starting as early as 3 days post infection (p.i.). the protection increased continuously up to 100% by 30 days p.i. neither stimulation of non-specific cell mediated mechanisms by previous bcg sensitization nor circulating interferon (ifn) seemed essential for such protection. the early detection of the virus in guinea pig organs considered the site of primary ... | 1985 | 2413752 |
| early protection to junín virus of guinea pig with an attenuated junín virus strain. | inoculation of guinea pigs with attenuated xjo junín virus (jv) strain confers protection against challenge with pathogenic xj-jv strain starting as early as 3 days post infection (p.i.). the protection increased continuously up to 100% by 30 days p.i. neither stimulation of non-specific cell mediated mechanisms by previous bcg sensitization nor circulating interferon (ifn) seemed essential for such protection. the early detection of the virus in guinea pig organs considered the site of primary ... | 1985 | 2413752 |
| splenic dendritic cells and junin virus. | | 1986 | 2425232 |
| interferon response in the guinea pig infected with junín virus. | the "in vivo" interferon (ifn) induction capacity of two junín virus strains--the attenuated xjcl3 and the intermediate virulent mc2--was studied in the guinea pig experimental model. three different doses of xjcl3 strain--2,000, 10,000, and 50,000 tcid50--and a single dose of 10,000 tcid50 of mc2 were assayed. animals were bled from day 0 to day 14 postinjection (pi) xjcl3 groups showed a constant serum ifn response. mc2 infection showed that 16% of the animals failed to develop interferonemia. ... | 1987 | 2445908 |
| interferon response in the guinea pig infected with junín virus. | the "in vivo" interferon (ifn) induction capacity of two junín virus strains--the attenuated xjcl3 and the intermediate virulent mc2--was studied in the guinea pig experimental model. three different doses of xjcl3 strain--2,000, 10,000, and 50,000 tcid50--and a single dose of 10,000 tcid50 of mc2 were assayed. animals were bled from day 0 to day 14 postinjection (pi) xjcl3 groups showed a constant serum ifn response. mc2 infection showed that 16% of the animals failed to develop interferonemia. ... | 1987 | 2445908 |
| antigenic relationships between attenuated and pathogenic strains of junin virus. | antigenic relationships between attenuated and pathogenic strains of junin virus (jv) were investigated. five strains of either human or rodent origin were tested by cross-neutralization assay with hyperimmune antisera, raised in rabbits, against each strain. polyclonal antisera could be used to distinguish among these jv strains, as the titer values differed significantly with ratios of homologous to heterologous titers, which ranged from 1.3 to 22.3. this demonstrates, independent of their vir ... | 1989 | 2466103 |
| junin virus monoclonal antibodies: characterization and cross-reactivity with other arenaviruses. | twenty-one monoclonal antibodies reactive with junin virus structural proteins were produced and characterized. using radioimmunoprecipitation and western blot assays, 13 were found to react with the nucleoprotein, seven with the surface glycoprotein and one failed to react, but showed a fluorescent antibody staining pattern consistent with other glycoprotein-specific antibodies. in radioimmunoprecipitation assays, glycoprotein-specific monoclonal antibodies reacted not only with the 35k structu ... | 1989 | 2471803 |
| reduced virulence of a junin virus mutant is associated with restricted multiplication in murine cells. | c167, a mutant derived from the xjc13 strain of junin virus, is highly attenuated in its pathogenic properties for newborn mice. whereas 10(2).pfu of xjc13 injected intracerebrally killed 100% of two-day-old mice, the mutant showed no detectable lethality. survival of mice infected with c167 was associated with a reduced and delayed virus replication in brain and a defective spread of virus from the site of inoculation to the other tissues, including spleen, kidney, thymus, liver, peritoneal cel ... | 1989 | 2479190 |
| calomys callidus as a potential junin virus reservoir. | the present study investigated whether c. callidus, a species belonging to the calomys genus, is capable of developing experimentally a persistent junin virus (jv) infection. newborn and adult cricetids were inoculated with the attenuated xj-clone 3 strain of jv by intracerebral or mucosal route. the present results indicate that the species is susceptible to jv infection, capable of shedding virus chronically through saliva and developing a persistent infection as shown by the detection of viru ... | 1989 | 2542433 |
| calomys callidus as a potential junin virus reservoir. | the present study investigated whether c. callidus, a species belonging to the calomys genus, is capable of developing experimentally a persistent junin virus (jv) infection. newborn and adult cricetids were inoculated with the attenuated xj-clone 3 strain of jv by intracerebral or mucosal route. the present results indicate that the species is susceptible to jv infection, capable of shedding virus chronically through saliva and developing a persistent infection as shown by the detection of viru ... | 1989 | 2542433 |
| actions of complement on junin virus. | fresh sera from normal rhesus monkeys, guinea pigs, and rabbits inactivated 90%-99% of the infectivity of vero cell-passaged, attenuated strains of junin virus (jv) within 60 minutes. selective depletion studies showed that inactivation occurred by the classical complement pathway. complement had little effect on virulent jv strains. adsorption of the fresh sera with jv-infected vero cells showed that inactivation was not mediated by low levels of antibodies in normal sera. the cells used for pr ... | 1989 | 2546249 |
| actions of complement on junin virus. | fresh sera from normal rhesus monkeys, guinea pigs, and rabbits inactivated 90%-99% of the infectivity of vero cell-passaged, attenuated strains of junin virus (jv) within 60 minutes. selective depletion studies showed that inactivation occurred by the classical complement pathway. complement had little effect on virulent jv strains. adsorption of the fresh sera with jv-infected vero cells showed that inactivation was not mediated by low levels of antibodies in normal sera. the cells used for pr ... | 1989 | 2546249 |
| nucleocapsid protein gene of junin arenavirus (cdna sequence). | | 1989 | 2552421 |
| protective effect of a low-dose of cyclophosphamide in experimental infection of guinea pigs with junin virus. | administration of cyclophosphamide (cy) to guinea pigs infected with a lethal strain of junin virus (jv) delayed the time of death, with survival of a small number of animals. virological studies showed a temporary decrease of virus concentration in blood and viscera shortly after the cy injection. in the pathological study no differences were found in the organic lesions present in cy-treated and nontreated animals, with the exception of the pulmonary alterations. in cy-treated guinea pigs the ... | 1989 | 2557385 |
| protective effect of a low-dose of cyclophosphamide in experimental infection of guinea pigs with junin virus. | administration of cyclophosphamide (cy) to guinea pigs infected with a lethal strain of junin virus (jv) delayed the time of death, with survival of a small number of animals. virological studies showed a temporary decrease of virus concentration in blood and viscera shortly after the cy injection. in the pathological study no differences were found in the organic lesions present in cy-treated and nontreated animals, with the exception of the pulmonary alterations. in cy-treated guinea pigs the ... | 1989 | 2557385 |
| formalin inactivated junin virus: immunogenicity and protection assays. | the aim of this study was to determine if junin virus inactivated with formalin (fa) was immunogenic and able to elicit a protective response in the guinea pig. the xj-clone 3 strain of junin virus grown in vero cells was exposed to fa at 0 degrees c. the following inactivated antigens were prepared: a1, 0.1% fa for 50 hr; a2, 0.1% fa for 50 hr followed by concentration with polyethylene glycol (peg); b1, 0.05% fa for 70 hr; b2, 0.05% fa for 70 hr plus peg concentration; c, 0.1% fa for 50 hr fol ... | 1989 | 2559158 |
| formalin inactivated junin virus: immunogenicity and protection assays. | the aim of this study was to determine if junin virus inactivated with formalin (fa) was immunogenic and able to elicit a protective response in the guinea pig. the xj-clone 3 strain of junin virus grown in vero cells was exposed to fa at 0 degrees c. the following inactivated antigens were prepared: a1, 0.1% fa for 50 hr; a2, 0.1% fa for 50 hr followed by concentration with polyethylene glycol (peg); b1, 0.05% fa for 70 hr; b2, 0.05% fa for 70 hr plus peg concentration; c, 0.1% fa for 50 hr fol ... | 1989 | 2559158 |
| [rapid serologic diagnosis of argentinian hemorrhagic fever in whole blood]. | the usefulness of a method for detection of antibodies against junin virus in whole blood was tested. n: nih adult mice were inoculated with 10(3) pfu of attenuated xj-clon 3 junin virus strain by intraperitoneal route and blood was obtained by retro-orbital puncture at 21 days post-infection. one blood aliquot (50 microliters) was collected in tubes containing a stabilizer solution for whole blood and another was processed for serum obtention. immunofluorescent antibodies were tested on spot sl ... | 1989 | 2559426 |
| astrocytic reaction predominance in chronic encephalitis of junin virus-infected rats. | junin virus antigen distribution and astrocytic reaction to prolonged infection were characterized in rat brain by the pap technique. during the acute stage of neurologic disease following intracerebral inoculation, junin antigen was detected in 100% of animals, strongly in most neurons but also to a much lesser degree in scattered astrocytes, dropping to 20% of rats at 540 days postinfection. initially labeled in all brain areas, viral antigen gradually disappeared from hippocampus but persiste ... | 1989 | 2559956 |
| [protection against encephalitis in rats caused by a pathogenic strain of the junin virus, using peripheral inoculation of an attenuated strain]. | argentine hemorrhagic fever manifests itself in man either subclinically or in hemorrhagic or neurological forms, mortality reaching 20%. although candid 1 strain is undergoing pilot trials, current therapy still resorts to convalescent serum administration. a neurological model was used to evaluate protection conferred by the attenuated xjc13 junin virus strain. newborn rats inoculated intraperitoneally (ip) prove resistant, whereas 8-12 day-old animals infected by intracerebral route with the ... | 1989 | 2562073 |
| macrophages are involved in age-dependent resistance of rats to junin virus infection. | we attempted to correlate rat age with resistance to intraperitoneal infection with the xj strain of junin virus. accordingly, mortality, viral replication in macrophages and brain, as well as neutralizing antibody (na) levels were recorded in animals inoculated at 2, 5, 10 and 26 days of life. two-day-old animals demonstrated both the greatest mortality (86%) and viral replication in macrophages, allowing virus to reach the brain where high titers were detected. this age group also had the high ... | 1987 | 2822597 |
| [role of macrophages in the dissemination of the junin virus in the rat]. | the 2-day-old rat is known to be susceptible to infection by ip route with the xj strain of junin virus but resists inoculation with xjc13 strain (85% vs 15% mortality). in order to determine whether peritoneal macrophages play a role in modulating the course of infection, viral replication in adherent peritoneal cells infected with either strain was studied. xj was found to replicate 30-fold as regards xjc13 at day 3 pi. besides, silica blockage of peritoneal macrophages was also evaluated. fol ... | 1985 | 2825238 |
| [susceptibility of 11-day-old mice to infection with the junin virus grown in different hosts]. | twelve clones derived from a stock of junin virus grown in baby mouse brain were isolated in vero cells. some properties of those viral clones were determined and compared with parental virus in order to ascertain the degree of heterogenicity of the original population. no differences were observed among clones and parental virus when the degree of thermolability was measured by heating them at 50 degrees c for 30 min. (table 1). similarly no ts phenotype character was present among all viral is ... | 1986 | 2825239 |
| [protection conferred by a hyperimmune serum and its fractions on rats infected with the junin virus]. | suckling rats infected by ic route with 10(3)ld50 of the xjc13 strain of jv were passively immunized with homologous hyperimmune serum (his). animals treated at 2 days pi with his showed a significant increase in survival vs. non-treated infected controls (82% vs 5%). however, at 4 days pi, transfer failed to modify survival. by means of deae sephadex a25 column chromatography, the presence of neutralizing immunoglobulin closely correlated with protective antibodies, but were not restricted to t ... | 1986 | 2825240 |
| antiviral effect of ribavirin on junin virus replication in vitro. | the effect of ribavirin (1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamide) on the replication in vitro of junin virus, the causative agent of argentine hemorrhagic fever (ahf), was examined. a concentration as low as 3.12 micrograms/ml of the drug inhibited the cytopathic effect observed five days post-infection (pi) on vero cells. on the other hand, a concentration of 25 micrograms/ml reduced the virus yield and specific antigen formation to undetectable levels. this same concentration of ri ... | 1986 | 2825242 |
| [attenuation in the virulence of a mutant of junin virus in suckling mice]. | the virulence in neonatal mice of a temperature-sensitive mutant of junin virus, named c167, was studied. the thermosensitive properties of this mutant were tested by titration on vero cells at 37 and 40 degrees c. the ratio of infectivity 40/37 was approximately 100-fold lower for c167 with respect to xjc13 (table 1). the attenuation of c167 was determined by measurement of mean survival time and 50% lethal dose after intracerebral injection of 2 and 11 day old mice. for c167 the lethality inde ... | 1987 | 2825245 |
| effect of persistent infection with junin virus on growth and reproduction of its natural reservoir, calomys musculinus. | the effect of infection with junin virus on growth and reproduction of its natural reservoir, calomys musculinus, was studied. eighty-five c. musculinus were inoculated intranasally at birth with 100 tcid50 of cba an 9446 strain of junin virus and observed for 480 days. no clinical signs of neurologic illness were registered. infected animals showed an increased mortality rate of up to 70% between days 24-40 post-infection. this period of high mortality was preceded by low weight gain during lac ... | 1987 | 2825553 |
| effect of persistent infection with junin virus on growth and reproduction of its natural reservoir, calomys musculinus. | the effect of infection with junin virus on growth and reproduction of its natural reservoir, calomys musculinus, was studied. eighty-five c. musculinus were inoculated intranasally at birth with 100 tcid50 of cba an 9446 strain of junin virus and observed for 480 days. no clinical signs of neurologic illness were registered. infected animals showed an increased mortality rate of up to 70% between days 24-40 post-infection. this period of high mortality was preceded by low weight gain during lac ... | 1987 | 2825553 |
| tacaribe virus: a new alternative for argentine hemorrhagic fever vaccine. | tacaribe virus is know to protect guinea pigs and primates against lethal challenge with junín virus. a long-term study on the effect of tacaribe virus infection in the guinea pig was carried out to determine the extent of cross-protection and whether antigen and/or viral persistence and tissue damage could be detected in immune animals. viral titers, antigen expression in organs, and histologic lesions were sequentially searched for up to 540 days postinfection (pi). neutralizing antibodies (ab ... | 1987 | 2828522 |
| tacaribe virus: a new alternative for argentine hemorrhagic fever vaccine. | tacaribe virus is know to protect guinea pigs and primates against lethal challenge with junín virus. a long-term study on the effect of tacaribe virus infection in the guinea pig was carried out to determine the extent of cross-protection and whether antigen and/or viral persistence and tissue damage could be detected in immune animals. viral titers, antigen expression in organs, and histologic lesions were sequentially searched for up to 540 days postinfection (pi). neutralizing antibodies (ab ... | 1987 | 2828522 |
| circulating interferon in the guinea pig infected with the xj, prototype junin virus strain. | the interferon (ifn) induction capacity of the xj prototype strain of junín virus (jv) was investigated in the guinea pig model. circulating alpha ifn was detected in 50% of the animals from days 2 to 9 postinfection (pi) and in 100% at day 11 pi, when all animals were in the premortem stage. individual levels ranged from 20 to 1,280 guinea pig ifn units (gpifnu)/ml. a correlation between xj strain virulence and ifn titers was recorded. a possible role of ifn as a pathogenic factor in the outcom ... | 1988 | 2828536 |
| circulating interferon in the guinea pig infected with the xj, prototype junin virus strain. | the interferon (ifn) induction capacity of the xj prototype strain of junín virus (jv) was investigated in the guinea pig model. circulating alpha ifn was detected in 50% of the animals from days 2 to 9 postinfection (pi) and in 100% at day 11 pi, when all animals were in the premortem stage. individual levels ranged from 20 to 1,280 guinea pig ifn units (gpifnu)/ml. a correlation between xj strain virulence and ifn titers was recorded. a possible role of ifn as a pathogenic factor in the outcom ... | 1988 | 2828536 |
| [association of the infection of the thymus and bone marrow with the establishment of persistent infection with junin virus in 2 genera of rodents]. | junin virus infection of immune system organs was correlated with persistence establishment in the mouse and rat. rockland mice under 24 or at 72 and 120 h of age received 10(4) pfu of junin virus xj strain by ic route. separately, two groups of mice under 24 h old were infected with the same dose of xj or xjcl3 strain by the same route respectively. results showed that higher thymus virus titer correlated with greater survival. in turn, the former also seemed to correlate with decreasing age at ... | 1985 | 2829274 |
| attenuation parameters for junin virus in the newborn rat. | to characterize a virus strain as attenuated, both biologic and biochemical criteria are necessary. in the case of junin virus, the 2-day-old rat has proved to be a biologic attenuation marker as regards mortality. here we studied the behaviour of the prototype xj vs the attenuated xjc13 strain inoculated by either ic or ip route to determine differential hematologic and splenic parameters. humoral immune response against srbc was also investigated. by either route xj caused significant leucocyt ... | 1985 | 2829275 |
| [isolation of junin virus from blood and peripheral lymphocytes of infected calomys musculinus]. | neonatal calomys musculinus experimental infection with junín virus (jv) xjcl3 strain causes either death or a persistent infection in the major part of surviving animals. jv can be isolated from peritoneal macrophages early during infection, and from brain and salivary glands during the chronic state of disease. it was of interest to investigate the appearance of virus in blood of infected animals. for this purpose, we decided to study the development of viremia in inoculated cricetids. a high ... | 1985 | 2829277 |
| [isolation of junin virus from blood and peripheral lymphocytes of infected calomys musculinus]. | neonatal calomys musculinus experimental infection with junín virus (jv) xjcl3 strain causes either death or a persistent infection in the major part of surviving animals. jv can be isolated from peritoneal macrophages early during infection, and from brain and salivary glands during the chronic state of disease. it was of interest to investigate the appearance of virus in blood of infected animals. for this purpose, we decided to study the development of viremia in inoculated cricetids. a high ... | 1985 | 2829277 |
| long-term protection against argentine hemorrhagic fever in tacaribe virus infected marmosets: virologic and histopathologic findings. | tacaribe virus may represent a better alternative than attenuated strains of junin virus (jv) for immunization against argentine hemorrhagic fever (ahf) because of possible risk of persistent infection of disease associated with live, attenuated strains. callithrix jacchus marmosets, which suffer 100% mortality if inoculated with the pathogenic xj strain of jv, were used to evaluate possible tacaribe virus persistence, subclinical, or long-term disease and the duration of protection against chal ... | 1988 | 2832541 |
| long-term protection against argentine hemorrhagic fever in tacaribe virus infected marmosets: virologic and histopathologic findings. | tacaribe virus may represent a better alternative than attenuated strains of junin virus (jv) for immunization against argentine hemorrhagic fever (ahf) because of possible risk of persistent infection of disease associated with live, attenuated strains. callithrix jacchus marmosets, which suffer 100% mortality if inoculated with the pathogenic xj strain of jv, were used to evaluate possible tacaribe virus persistence, subclinical, or long-term disease and the duration of protection against chal ... | 1988 | 2832541 |
| evaluation of an enzyme-linked immunosorbent assay for quantitation of antibodies to junin virus in human sera. | an enzyme-linked immunosorbent assay (elisa) was evaluated for the quantitation of anti-junin virus (jv) antibodies, in 83 selected cases of argentine haemorrhagic fever (ahf). serum samples were studied in two groups to facilitate comparative analysis; the first group was elisa with indirect immunofluorescence (if) test, in the second elisa with plaque reduction neutralization test (print). from the results obtained by using elisa and if on the same serum samples, a clear tendency of elisa to d ... | 1988 | 2836465 |
| is vertical transmission sufficient to maintain junin virus in nature? | the quantitative contribution of vertical transmission to the prevalence rate of junin virus infection in subsequent generations of its natural reservoir, calomys musculinus, was analysed. data on mortality and reproduction of c. musculinus infected at birth with a wild strain of junin virus were used to estimate the infection-dependent relative survival rate (beta = 0.4849) and relative fertility of the infected host (alpha = 0.2088). prevalence rates of infection, obtained by mathematical simu ... | 1988 | 2838581 |
| is vertical transmission sufficient to maintain junin virus in nature? | the quantitative contribution of vertical transmission to the prevalence rate of junin virus infection in subsequent generations of its natural reservoir, calomys musculinus, was analysed. data on mortality and reproduction of c. musculinus infected at birth with a wild strain of junin virus were used to estimate the infection-dependent relative survival rate (beta = 0.4849) and relative fertility of the infected host (alpha = 0.2088). prevalence rates of infection, obtained by mathematical simu ... | 1988 | 2838581 |
| junin virus-induced delayed-type hypersensitivity suppression in adult mice. | junin virus (jv) infection of suckling mice leads to lethal meningoencephalitis consistent with a delayed-type hypersensitivity (dth)-like immune response. in contrast, there are no central nervous system (cns) alterations, and high antibody titers are induced in resistant adult mice. as a possible explanation, jv infection in adult mice may provoke dth depression. thus in this work we study the alterations induced by jv in the immune response of adult mice by using sheep red blood cells (srbc) ... | 1988 | 2839614 |
| junin virus-induced delayed-type hypersensitivity suppression in adult mice. | junin virus (jv) infection of suckling mice leads to lethal meningoencephalitis consistent with a delayed-type hypersensitivity (dth)-like immune response. in contrast, there are no central nervous system (cns) alterations, and high antibody titers are induced in resistant adult mice. as a possible explanation, jv infection in adult mice may provoke dth depression. thus in this work we study the alterations induced by jv in the immune response of adult mice by using sheep red blood cells (srbc) ... | 1988 | 2839614 |
| epidemiology of argentine hemorrhagic fever. | present knowledge points to horizontal transmission as the most significant mechanism for junín virus maintenance in the main natural reservoirs, namely calomys musculinus and calomys laucha. the existence of naturally infected akodon azarae, both within and outside the endemic area, as well as the finding that other species, ecologically and phylogenetically related to the main reservoirs, such as akodon molinae and calomys callidus, can experimentally develop persistent infections with virus s ... | 1988 | 2841151 |
| epidemiology of argentine hemorrhagic fever. | present knowledge points to horizontal transmission as the most significant mechanism for junín virus maintenance in the main natural reservoirs, namely calomys musculinus and calomys laucha. the existence of naturally infected akodon azarae, both within and outside the endemic area, as well as the finding that other species, ecologically and phylogenetically related to the main reservoirs, such as akodon molinae and calomys callidus, can experimentally develop persistent infections with virus s ... | 1988 | 2841151 |
| differentiation of junin virus and antigenic variants isolated in vivo by kinetic neutralization assays. | the major natural reservoir of junin virus, the aetiological agent of argentine haemorrhagic fever, is the cricetid calomys musculinus. neonatal animals experimentally infected with junin virus (xjcl3 strain) developed typical disease and approximately 80% of them died. most survivors become persistently infected. antigenically variant viruses were isolated from the blood and brain of infected cricetids during the acute and chronic stages of the disease. these variants could be distinguished fro ... | 1988 | 2841418 |
| differentiation of junin virus and antigenic variants isolated in vivo by kinetic neutralization assays. | the major natural reservoir of junin virus, the aetiological agent of argentine haemorrhagic fever, is the cricetid calomys musculinus. neonatal animals experimentally infected with junin virus (xjcl3 strain) developed typical disease and approximately 80% of them died. most survivors become persistently infected. antigenically variant viruses were isolated from the blood and brain of infected cricetids during the acute and chronic stages of the disease. these variants could be distinguished fro ... | 1988 | 2841418 |
| mortality induced by adoptive immunity in junin virus-infected athymic mice. | the effect of normal or sensitized spleen cell transfer from syngeneic euthymic mice to junin virus-infected suckling athymic mice was studied. transfer was performed 1 or 7 days after infection. in both cases, an acute lethal disease developed 6-11 days after transfer. the mortality reached 100% in all infected groups receiving normal or sensitized splenocytes, while it was negligible for different control groups of athymic mice. transfer of normal or sensitized splenocytes was unable to signif ... | 1988 | 2842272 |
| viral strain dependent differences in experimental argentine hemorrhagic fever (junin virus) infection of guinea pigs. | guinea pigs infected with low-passage junin virus of human origin showed viral strain dependent differences in mortality, ld50, time to death, and in viral spread and distribution. different junin strains appeared to cause at least two broad patterns of argentine hemorrhagic fever in guinea pigs. a number of strains of junin virus caused a viscerotropic type of illness in which virus replicated predominantly in lymph nodes, spleen, and bone marrow. with the most severe visceral forms of argentin ... | 1988 | 2846464 |
| viral strain dependent differences in experimental argentine hemorrhagic fever (junin virus) infection of guinea pigs. | guinea pigs infected with low-passage junin virus of human origin showed viral strain dependent differences in mortality, ld50, time to death, and in viral spread and distribution. different junin strains appeared to cause at least two broad patterns of argentine hemorrhagic fever in guinea pigs. a number of strains of junin virus caused a viscerotropic type of illness in which virus replicated predominantly in lymph nodes, spleen, and bone marrow. with the most severe visceral forms of argentin ... | 1988 | 2846464 |
| ribavirin effect on experimental junin virus-induced encephalitis. | junin virus, the etiological agent of argentine hemorrhagic fever, produces in man a disease mainly characterized by hemorrhagic alterations, commonly accompanied by neurological symptoms, and leading to 10% mortality. intracerebral inoculation in 10-day-old rats or intraperitoneal inoculation in 2-day-old rats leads to high mortality due to severe encephalitis. here, the effect of ribavirin on these experimental models was tested in order to evaluate the degree of protection achieved against ne ... | 1988 | 2846772 |
| ribavirin prophylaxis and therapy for experimental argentine hemorrhagic fever. | junin virus-infected rhesus macaques received prophylactic and therapeutic ribavirin to assess the potential of this drug for treating humans with argentine hemorrhagic fever. when ribavirin was administered intramuscularly at the time of experimental infection with the lethal p3790 strain of junin virus, all animals were protected from clinical disease. a delay in the initiation of therapy until after the onset of illness resulted in improvement and resolution of systemic signs of disease; howe ... | 1988 | 2848441 |
| ribavirin prophylaxis and therapy for experimental argentine hemorrhagic fever. | junin virus-infected rhesus macaques received prophylactic and therapeutic ribavirin to assess the potential of this drug for treating humans with argentine hemorrhagic fever. when ribavirin was administered intramuscularly at the time of experimental infection with the lethal p3790 strain of junin virus, all animals were protected from clinical disease. a delay in the initiation of therapy until after the onset of illness resulted in improvement and resolution of systemic signs of disease; howe ... | 1988 | 2848441 |
| lectin affinity of junin virus glycoproteins. | we studied the binding of junin virus (arenaviridae) glycoproteins, g1 and g2, to two insolubilized lectins. the results showed that mannose, n-acetyl-glucosamine and galactose residues were exposed on g2, while only the latter predominated on g1. heterogeneity of carbohydrate chains was found in g2, the only glycoprotein that was iodinated by the lactoperoxidase method. | 1988 | 2849965 |
| susceptible adult murine model for junin virus. | the adult mouse model had been considered resistant to junin virus (jv) infection. however, we found that c3h/hej murine strain proved highly susceptible up to 5 months of age to intracerebral inoculation with the prototype xj jv strain, showing neurological signs and 80-90% mortality within 13 days. neutralizing antibodies (nt ab) were absent, but low immunofluorescent ab levels (1:5) were detected as from day +7. the virus could only be rescued by coculture of brain samples with vero cells. hi ... | 1988 | 2850346 |
| susceptible adult murine model for junin virus. | the adult mouse model had been considered resistant to junin virus (jv) infection. however, we found that c3h/hej murine strain proved highly susceptible up to 5 months of age to intracerebral inoculation with the prototype xj jv strain, showing neurological signs and 80-90% mortality within 13 days. neutralizing antibodies (nt ab) were absent, but low immunofluorescent ab levels (1:5) were detected as from day +7. the virus could only be rescued by coculture of brain samples with vero cells. hi ... | 1988 | 2850346 |
| [heterogeneity in the virulence of viral subpopulations derived from an attenuated strain of junin virus]. | | 1987 | 2854292 |
| cytolysis of junin infected target cells by immune guinea pig spleen cells. | spleen cells from guinea pigs infected with an attenuated strain of junin virus (the causative agent of argentine hemorrhagic fever) specifically lysed virus-infected syngeneic target cells in vitro. this activity was detected as early as 6 days after infection, reached a maximum on days 10-13, and persisted at lower levels, at least through day 30. monoclonal antibody to guinea pig t cells had no effect on the activity. after b or t cell enrichment techniques, the cytolysis was found with the b ... | 1986 | 2854602 |
| cytolysis of junin infected target cells by immune guinea pig spleen cells. | spleen cells from guinea pigs infected with an attenuated strain of junin virus (the causative agent of argentine hemorrhagic fever) specifically lysed virus-infected syngeneic target cells in vitro. this activity was detected as early as 6 days after infection, reached a maximum on days 10-13, and persisted at lower levels, at least through day 30. monoclonal antibody to guinea pig t cells had no effect on the activity. after b or t cell enrichment techniques, the cytolysis was found with the b ... | 1986 | 2854602 |
| polypeptide synthesis in junín virus-infected bhk-21 cells. | analysis by immune precipitation and sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page) of intracellular [35s]-methionine-labelled specific junín virus polypeptides demonstrated synthesis of the nucleoprotein (np64) between 24 to 96 hr post-infection (p.i.). two glycoproteins with apparent molecular weights (mr) of 72,000 (gp72) and 38,000 (gp38) were detected by pulse labelling with [35s]-methionine or sugar precursor at about 48 hr p.i. the rate of synthesis of gp38 increase ... | 1985 | 2860803 |
| junín virus persistence in mice. | newborn mice surviving intracerebral infection with junín virus (jv) strain xj showed viral persistence in brain up to 140 days post-infection (p.i.). mild meningoencephalitis or encephalitis, but not the neutralizing antibody titres (ntab) correlated with virus presence. | 1987 | 2883864 |
| abrogation of junin virus encephalitis by critical cyclophosphamide timing and dosage. | junin virus-induced encephalitis in suckling mouse is a delayed-type hypersensitivity reaction, whose immunopathologic nature has been proven by suppressing the thymus-dependent response. cyclophosphamide (cy) given at day +6 post-infection (p.i.) has been shown to modulate infection, presumably by tdth lymphocyte inactivation. to determine critical timing and i.p. drug dose, brain histology and survival were studied in 3-day-old balb/c mice, inoculated i.c. with junin virus. optimal protection ... | 1987 | 2888291 |
| in vivo junin virus-mouse macrophages interaction. | the role of mononuclear phagocytic cells in extraneural infection of the mouse with junin virus (jv) was studied. endpoint susceptibility (4 days of life) was evaluated by intraperitoneal (i.p.) inoculation of suckling mice. by means of immunofluorescence (if) and c3 receptor assays, it was found that macrophages were permissive to viral replication in vivo and fostered the recruitment of inflammatory cells as evidenced by the absence of c3 marker. in support, in vitro infection failed to induce ... | 1988 | 2902768 |
| suppressor t-cell population induced by junin virus in adult mice. | intracerebral (i.c.) junin virus (jv) infection of adult balb/c mice is characterized by the absence of morbidity and a low mortality (barely 8-10%). in contrast, the suckling mouse model exhibits almost 100% mortality following central nervous system (cns) alterations consistent with a delayed-type hypersensitivity (dth)-like immune response. besides, jv infection of adult (resistant) mice leads to immunosuppression of dth to unrelated antigens. here we present evidence demonstrating that such ... | 1988 | 2970429 |
| effect of immunosuppression on experimental argentine hemorrhagic fever in guinea pigs. | immunosuppression with cyclosporin a or cyclophosphamide had no apparent effect on the disease course of guinea pigs infected with a virulent strain of junin virus. immunosuppression of guinea pigs infected with an attenuated strain of junin virus led to fulminating argentine hemorrhagic fever. all immunosuppressed infected animals died. virus distribution patterns in target organs, as determined by plaque assay and fluorescent antibody procedures, were similar to those from non-immunosuppressed ... | 1985 | 2981364 |
| effect of immunosuppression on experimental argentine hemorrhagic fever in guinea pigs. | immunosuppression with cyclosporin a or cyclophosphamide had no apparent effect on the disease course of guinea pigs infected with a virulent strain of junin virus. immunosuppression of guinea pigs infected with an attenuated strain of junin virus led to fulminating argentine hemorrhagic fever. all immunosuppressed infected animals died. virus distribution patterns in target organs, as determined by plaque assay and fluorescent antibody procedures, were similar to those from non-immunosuppressed ... | 1985 | 2981364 |
| attenuated junin virus infection in callithrix jacchus. | twenty marmosets, male callithrix jacchus, were used during this study. fifteen of the marmosets were inoculated with 5,000 tcid50 of the attenuated xjc13 strain of junin virus by intramuscular route and five were left as uninoculated controls. animals were observed for a 420-day period. in order to carry out virologic, hematologic, serologic, and histologic studies the animals were bled and/or killed at different days post infection(pi). results obtained showed that the attenuated strain produc ... | 1985 | 2981980 |
| attenuated junin virus infection in callithrix jacchus. | twenty marmosets, male callithrix jacchus, were used during this study. fifteen of the marmosets were inoculated with 5,000 tcid50 of the attenuated xjc13 strain of junin virus by intramuscular route and five were left as uninoculated controls. animals were observed for a 420-day period. in order to carry out virologic, hematologic, serologic, and histologic studies the animals were bled and/or killed at different days post infection(pi). results obtained showed that the attenuated strain produc ... | 1985 | 2981980 |
| effect of staggered cyclophosphamide-immunosuppression on resistance to experimental junin virus infection. | otherwise resistant adult mice were rendered susceptible to intracerebral junin virus (jv) infection only when a staggered cyclophosphamide (cy) schedule was used. forty-five-day old balb/c mice, intracerebrally jv-infected and immunosuppressed with four 50 mg/kg body weight cy doses at days -1, +1, +4, +6 (day 0: viral infection) developed a lethal disease (86.6 per cent mortality) with high cns viral titers and brain lesions. neutralizing antibodies were absent throughout, while immunofluoresc ... | 1985 | 2982356 |
| interactions of junin and tacaribe viruses during mixed infections. | the interaction between junin virus (jv) and tacaribe virus (tacv) during mixed infections of rk13 cells was examined. the effects of a prior infection with jv upon tacv replication depended on the time between the two inoculations. simultaneous infection of rk13 cells with tacv and jv did not alter the plaquing efficiency of tacv; but if there was a 1- to 24-hour delay between jv preinfection and tacv superinfection, a variable increase of tacv replication was observed. the enhancement of tacv ... | 1985 | 2982755 |
| infection of pregnant guinea pigs with attenuated junin virus strains. | the effect of the attenuated xjc13 and xj0 strains of junin virus (jv) was studied in guinea pigs infected before and during pregnancy. the 58% mortality rate in animals infected during gestation and the 16.7% mortality rate in chronically infected animals were attributed to a viral effect. an abortion rate of 33% occurred in animals infected before the 7th week of gestation. regardless of the time of infection, jv was isolated from central nervous system tissue, placentas, and fetuses of animal ... | 1985 | 2982762 |
| infection of pregnant guinea pigs with attenuated junin virus strains. | the effect of the attenuated xjc13 and xj0 strains of junin virus (jv) was studied in guinea pigs infected before and during pregnancy. the 58% mortality rate in animals infected during gestation and the 16.7% mortality rate in chronically infected animals were attributed to a viral effect. an abortion rate of 33% occurred in animals infected before the 7th week of gestation. regardless of the time of infection, jv was isolated from central nervous system tissue, placentas, and fetuses of animal ... | 1985 | 2982762 |
| neurotropism of a high-passage xj strain of junín virus. | guinea pig infection with a highly passaged xj prototype strain of junín virus by the intramuscular route (im) was carried out in order to study viral tropism modification in this host. the neurotropism of this strain was demonstrated by viral isolation, meningitis, and by the presence of junín antigens as shown by immunofluorescence. these events, not previously observed with the same lower-passaged strain, revealed the appearance of neurotropism after multiple passages in guinea pigs. | 1985 | 2983010 |
| neurotropism of a high-passage xj strain of junín virus. | guinea pig infection with a highly passaged xj prototype strain of junín virus by the intramuscular route (im) was carried out in order to study viral tropism modification in this host. the neurotropism of this strain was demonstrated by viral isolation, meningitis, and by the presence of junín antigens as shown by immunofluorescence. these events, not previously observed with the same lower-passaged strain, revealed the appearance of neurotropism after multiple passages in guinea pigs. | 1985 | 2983010 |
| pathogenesis of attenuated junin virus in the guinea pig model. | the purpose of this work was to elucidate the pathogenesis of attenuated junin virus (jv) strains in the guinea pig model. three groups of guinea pigs were infected by the im route with 10(3) pfu of the xjc13 and xjo-attenuated strains or with the xj pathogenic strain of jv, respectively. viremia was studied at 3, 5, 7, 9, 12, and 14 days postinfection (pi) (a) in serum samples of all animals and in washed cells from xjc13-infected guinea pigs by conventional techniques and (b) in whole blood sa ... | 1985 | 2983013 |
| pathogenesis of attenuated junin virus in the guinea pig model. | the purpose of this work was to elucidate the pathogenesis of attenuated junin virus (jv) strains in the guinea pig model. three groups of guinea pigs were infected by the im route with 10(3) pfu of the xjc13 and xjo-attenuated strains or with the xj pathogenic strain of jv, respectively. viremia was studied at 3, 5, 7, 9, 12, and 14 days postinfection (pi) (a) in serum samples of all animals and in washed cells from xjc13-infected guinea pigs by conventional techniques and (b) in whole blood sa ... | 1985 | 2983013 |
| mrc-5 cells, a model for junín virus persistent infection. | persistent infection of mrc-5 cells was established following inoculation with attenuated junín virus (jv). in the acute phase of the infection both the pathogenic xj and the attenuated xj0 and xjc13 strains showed severe c.p.e. and free viral titres reached 10(5) p.f.u./ml. recovery and establishment of persistently infected mrc-5 sublines (mrc-5pi) proved a very common event and seemed to be independent of viral strain, m.o.i. employed or virus passage history. these mrc-5pi sublines released ... | 1985 | 2987401 |
| congenital guinea pig infection with attenuated junin virus strains. | guinea pigs born from mothers infected before or during pregnancy with 10(3) pfu of the attenuated xjc13 or xj0 strains of junin virus (jv) by the intramuscular route showed 31.5% mortality that was not attributable to the mothers' clinical condition or to lack of care. there was a slight drop in mortality rate when the mothers were infected at the beginning or end of their gestation period. jv isolation from the 9 offspring killed from 1 to 125 days of age proved that virus transmitted transpla ... | 1985 | 2989214 |
| congenital guinea pig infection with attenuated junin virus strains. | guinea pigs born from mothers infected before or during pregnancy with 10(3) pfu of the attenuated xjc13 or xj0 strains of junin virus (jv) by the intramuscular route showed 31.5% mortality that was not attributable to the mothers' clinical condition or to lack of care. there was a slight drop in mortality rate when the mothers were infected at the beginning or end of their gestation period. jv isolation from the 9 offspring killed from 1 to 125 days of age proved that virus transmitted transpla ... | 1985 | 2989214 |
| astrocyte differentiation induced by junín virus in rat brain cell cultures. | morphological and immunocytochemical differentiation was observed in astroglial cell cultures of the rat infected with junín virus. from days 3 to 6 postinoculation (p.i.), gfap immunostaining was observed in both the perikaryon and processes of maturated astrocytes, whereas it was limited to the perikaryon in less differentiated cells. the rather slow spontaneous differentiation usually occurring in astroglial cell cultures was seen to be accelerated by viral infection, mimicking the astrocytic ... | 1985 | 2990148 |
| influence of cellular functions on the evolution of persistent infections with junin virus. | vero cell cultures persistently infected with junin virus and subjected to different cultural conditions were established. the production of infectious plaque-forming virus, ts mutants and interfering viral particles was determined at different times during 110 days after infection. carrier cultures maintained in stationary conditions continuously released pfu while proliferating persistent cultures exhibited a cyclical pattern which tends to a rapid pfu disappearance. concomitantly, in stationa ... | 1985 | 2998303 |
| junin virus-induced chromosomal aberrations in the guinea pig. synergism between the attenuated strain xj-clone 3 and caffeine. | the frequency of chromosomal aberrations in bone marrow cells of guinea pigs inoculated with the pathogenic xj strain of junin virus increased significantly at 6, 9, and 11 days postinoculation (p.i.). animals inoculated with the attenuated xj-clone 3 strain only showed significant increments of achromatic lesions (gaps) at 9 days p.i. guinea pigs inoculated with the xj-clone 3 strain and then treated with two doses of caffeine 24 and 12 h before killing at 9 days p.i. exhibited a significant in ... | 1985 | 3000979 |
| junin virus-induced chromosomal aberrations in the guinea pig. synergism between the attenuated strain xj-clone 3 and caffeine. | the frequency of chromosomal aberrations in bone marrow cells of guinea pigs inoculated with the pathogenic xj strain of junin virus increased significantly at 6, 9, and 11 days postinoculation (p.i.). animals inoculated with the attenuated xj-clone 3 strain only showed significant increments of achromatic lesions (gaps) at 9 days p.i. guinea pigs inoculated with the xj-clone 3 strain and then treated with two doses of caffeine 24 and 12 h before killing at 9 days p.i. exhibited a significant in ... | 1985 | 3000979 |
| brain inflammatory exudate in junin virus-infected rats: its characterization by the immunoperoxidase (pap) technique. | morphologic changes in cyclophosphamide (cy)-suppressed vs. control non-suppressed new-born rats infected i.c. with xjc13 strain of junin virus were compared and the cells involved in cns lesions were identified by the pap technique. fifty per cent of the control rats exhibited widespread cerebral necrosis vs. only 15% of the immunosuppressed animals. the first cells to reach junin virus-infected cns in controls were t lymphocytes, which destroyed viral antigen-laden target neurons and astrocyte ... | 1986 | 3005359 |
| administration of antithymocyte serum modifies the response of calomys musculinus to junin virus infection. | approximately 80% of calomys musculinus inoculated with an attenuated strain of junin virus (jv) developed a lethal encephalitis. antithymocyte serum, a potent suppressor of t-cell-mediated immunity, was studied for its effect on jv pathogenicity. early administration of an anti-c. musculinus thymocyte serum (acts) to neonatal animals significantly diminished clinical disease and death and abrogated brain damage, which is usually associated with viral presence in the brain. late acts administrat ... | 1986 | 3009353 |
| administration of antithymocyte serum modifies the response of calomys musculinus to junin virus infection. | approximately 80% of calomys musculinus inoculated with an attenuated strain of junin virus (jv) developed a lethal encephalitis. antithymocyte serum, a potent suppressor of t-cell-mediated immunity, was studied for its effect on jv pathogenicity. early administration of an anti-c. musculinus thymocyte serum (acts) to neonatal animals significantly diminished clinical disease and death and abrogated brain damage, which is usually associated with viral presence in the brain. late acts administrat ... | 1986 | 3009353 |
| role of calomys musculinus peritoneal macrophages in age-related resistance to junin virus infection. | in nature, the cricetid calomys musculinus is the principal host of junin virus, the etiological agent of argentine hemorrhagic fever. in the experimental infection, adult c. musculinus survived whereas newborns died after intraperitoneal inoculation with the xj.cl3 strain of junin virus. the role of peritoneal macrophages in this age-related resistance was studied. junin virus multiplied in cultivated macrophages from either neonatal or adult animals and, therefore, it was not possible to corre ... | 1986 | 3009700 |
| role of calomys musculinus peritoneal macrophages in age-related resistance to junin virus infection. | in nature, the cricetid calomys musculinus is the principal host of junin virus, the etiological agent of argentine hemorrhagic fever. in the experimental infection, adult c. musculinus survived whereas newborns died after intraperitoneal inoculation with the xj.cl3 strain of junin virus. the role of peritoneal macrophages in this age-related resistance was studied. junin virus multiplied in cultivated macrophages from either neonatal or adult animals and, therefore, it was not possible to corre ... | 1986 | 3009700 |