| listeria monocytogenes in products of animal origin in turkey. | a study was carried out on 430 samples of different foodstuffs (soft cheese, raw chicken, minced beef, sausage, fish) and 400 carcase samples (sheep, young and adult cattle) for screening of listeria monocytogenes. it was found that only one of the samples contained l. monocytogenes at > 10(3) cfu/ml in the initial examination, but another 42 samples contained l. monocytogenes following an enrichment process. l. monocytogenes was isolated most frequently from raw chicken samples (18%), but was n ... | 2004 | 15563103 |
| specific detection of cytopathogenic listeria monocytogenes using a two-step method of immunoseparation and cytotoxicity analysis. | the development of rapid methods for detection of viable listeria monocytogenes is crucial to prevent listeriosis and product recalls. while immunomagnetic separation has been used for isolating listeria spp., lack of specificity and pathogenicity determination render this method unsatisfactory. a two-step method using protein a agarose beads (immunobeads) coated with a more specific antibody, monoclonal antibody (mab)-c11e9 for l. monocytogenes was developed. immunobeads were allowed to capture ... | 2005 | 15590100 |
| truncated internalin a and asymptomatic listeria monocytogenes carriage: in vivo investigation by allelic exchange. | allelic exchange of the region coding for the c terminus of inla between one epidemic (with an 80-kda inla) and one asymptomatic (with a 47-kda inla) carriage listeria monocytogenes strain confirmed the need for this region for internalin entry in vitro. interestingly, restoration of internalin a functionality did not result in full virulence in chicken embryo assays. | 2005 | 15618209 |
| characterization of a mutant listeria monocytogenes strain expressing green fluorescent protein. | to construct a recombinant strain of listeria monocytogenes for the expression of heterologous genes, homologous recombination was utilized for insertional mutation, targeting its listeriolysin o gene (hly). the gene encoding green fluorescent protein (gfp) was used as the indicator of heterologous gene expression. the gene gfp was inserted into hly downstream from its promoter and signal sequence by an overlapping extension polymerase chain reaction, and was then cloned into the shuttle plasmid ... | 2005 | 15645077 |
| carrier status for listeria monocytogenes and other listeria species in free range farm and market healthy indigenous chickens and ducks. | listeria organisms are documented to be zoonotic; one of the sources of infection is the domestic fowl where it could occur as in apparent infection. the carriage of listeria monocytogenes and other listeria in indigenous birds has not been documented in kenya. | 2004 | 15715131 |
| sensitivity of listeria species, recovered from indigenous chickens to antibiotics and disinfectants. | resistance of bacteria to antibiotics and disinfectants has been reported widely in the world. listeria monocytogenes is no exception, although normally it tends to be variably sensitive to many antibiotics and disinfectants. | 2004 | 15715132 |
| contamination of chicken carcasses in gauteng, south africa, by salmonella, listeria monocytogenes and campylobacter. | the presence of the foodborne pathogens, salmonella spp., listeria monocytogenes and campylobacter spp., on 99 fresh and frozen chicken carcasses sourced from various retailers in gauteng, south africa, was investigated. using culture methods, 60.6% of the carcasses were found to be contaminated with one or more pathogens, with 19.2%, 19.2% and 32.3% of the carcasses being found to harbour salmonella, l. monocytogenes and campylobacter, respectively. the extent of contamination with one or more ... | 2005 | 15718024 |
| simultaneous detection of listeria monocytogenes in chicken meat enrichments by pcr and reverse-transcription pcr without dna/rna isolation. | environmental and food samples can be analyzed using pcr and reverse transcription (rt)-pcr techniques to discriminate between viable and nonviable cells of bacterial pathogens. here, we describe the use of a commercial lysis buffer, initially designed for mammalian cells, that permits the rapid extraction of bacterial dna and rna. the buffer is an rt-pcr-compatible lysis solution in which rna is stable and can be frozen for later use. rt-pcr is carried out directly after dnase i treatment of cr ... | 2005 | 15726988 |
| the synthetic form of a novel chicken beta-defensin identified in silico is predominantly active against intestinal pathogens. | antimicrobial peptides are essential components of innate immunity and are generally thought to act by disrupting the membrane integrity of microbes. here we report the discovery of two novel chicken beta-defensins, gallinacin (gal)-11 and gal-12, found by hidden markov model profile searching of the chicken genome. we have sequenced the genes and elucidated the 3'utr of gal-11. differential mrna expression of these novel genes has been shown across a panel of chicken tissues. gal-11 mrna was hi ... | 2005 | 15744537 |
| development of a sandwich elisa for the detection of listeria spp. using specific flagella antibodies. | five monoclonal antibodies (mabs) and chicken immunoglobulin (igy) were developed by immunizing with flagella purified from listeria monocytogenes 4b and the five mabs have been confirmed to be specific against three different epitopes of flagellin. the antibodies showed specific reaction to listeria genus and no cross-reactivity with other bacteria tested in this experiment including e.coli o157:h7 and salmonella enteritidis. sandwich enzyme-linked immunosorbent assays (elisa) using the mabs an ... | 2005 | 15785122 |
| monochloramine versus sodium hypochlorite as antimicrobial agents for reducing populations of bacteria on broiler chicken carcasses. | studies were conducted to compare the effect of sodium hypochlorite (sh) versus monochloramine (mon) on bacterial populations associated with broiler chicken carcasses. in study 1, nominal populations (6.5 to 7.5 log cfu) of escherichia coli, listeria monocytogenes, pseudomonas fluorescens, salmonella serovars, shewanella putrefaciens, and staphylococcus aureus were exposed to sterilized chiller water (controls) or sterilized chiller water containing 50 ppm sh or mon. sh at 50 ppm eliminated all ... | 2005 | 15830667 |
| effectiveness of trisodium phosphate treatment against pathogenic and spoilage bacteria on poultry during refrigerated storage. | to determine the efficacy of trisodium phosphate (tsp) against pathogenic and spoilage bacteria on poultry and to assess the influence of the bacterial combination of inoculum on tsp effect, chicken legs were coinoculated with similar concentrations of a pathogenic (salmonella enterica ser. enteritidis or listeria monocytogenes) and a spoilage (pseudomonas fluorescens or brochothrix thermosphacta) bacteria. samples were dipped in tsp (12%, 15 min) or were not treated (control). microbiological a ... | 2005 | 15830686 |
| distribution of listeria monocytogenes subtypes within a poultry further processing plant. | samples from environmental sites and raw product in a chicken further processing plant were collected every 6 weeks for 12 months. each sample site was examined before and after a complete production shift. all samples were examined for the presence of listeria monocytogenes, which was detected in floor drains on the raw product side of the plant preoperation and in drains on both raw and cooked sides following 8 h of processing operation. l. monocytogenes also was detected in raw product and on ... | 2005 | 15895730 |
| bacteriophage control of foodborne bacteriat. | bacteriophages are measurable components of the natural microflora in the food production continuum from the farm to the retail outlet. phages are remarkably stable in these environments and are readily recovered from soil, sewage, water, farm and processing plant effluents, feces, and retail foods. purified high-titer phage lysates have been used for the species-specific control of bacteria during the pre- and postharvest phases of food production and storage. for example, the inhibition of the ... | 2005 | 15895751 |
| detection of pathogenic bacteria in food samples using highly-dispersed carbon particles. | there is an unmet need for detection methods that can rapidly and sensitively detect food borne pathogens. a flow through immunoassay system utilizing highly dispersed carbon particles and an amperometric technique has been developed and optimized. a sandwich immunoassay format was utilized in which pathogenic cells were captured by antibodies immobilized onto activated carbon particles, and labeled with horseradish peroxidase (hrp) conjugated antibodies. flow of the peroxidase substrates result ... | 2005 | 16076439 |
| discrimination of listeria monocytogenes contaminated commercial japanese meats. | discrimination was attempted on 14 listeria monocytogenes strains isolated from commercially available japanese pork and chicken. examination of the isolates was performed by restriction fragment length polymorphism (rflp) analysis of the chromosomal dna and amplified products and comparison of the nucleotide sequences of the amplified products. a polymorphism region containing the repeated sequences in the iap gene was amplified by the polymerase chain reaction (pcr). the genetic analyses could ... | 2005 | 16091297 |
| behavior of listeria monocytogenes in ph-modified chicken salad during refrigerated storage. | the growth and inactivation kinetics of l. monocytogenes were evaluated at ph 4.0, 4.6, and 5.2 during storage at 5.0 degrees c, 7.2 degrees c, and 21.1 degrees c (41 degrees f, 45 degrees f, and 70 degrees f). using commercially produced pasteurized chicken salad, the authors adjusted the ph levels with acetic acid or sodium acetate. samples of 25 g each of the ph-modified salad were inoculated to approximately 1 x 10(6) cells per gram with a three-strain mixture of l. monocytogenes and stored ... | 2005 | 16121485 |
| occurrence of campylobacter and listeria monocytogenes in a poultry processing plant. | the occurrence of campylobacter and listeria monocytogenes was studied in 645 samples from surfaces, water, and poultry products (chicken carcasses, chicken parts, viscera, and spoils) in a poultry processing plant in southern brazil. the automated mini-vidas system was used to detect the presence of campylobacter and l. monocytogenes on the samples. the positive samples were confirmed by conventional methods. campylobacter and l. monocytogenes were found in 16.6 and 35.6% of the analyzed sample ... | 2005 | 16161691 |
| effectiveness of antimicrobial food packaging materials. | antimicrobial additives have been used successfully for many years as direct food additives. the literature provides evidence that some of these additives may be effective as indirect food additives incorporated into food packaging materials. antimicrobial food packaging is directed toward the reduction of surface contamination of processed, prepared foods such as sliced meats and frankfurter sausages (hot dogs). the use of such packaging materials is not meant to be a substitute for good sanita ... | 2005 | 16227182 |
| adhesion of selected bifidobacterium strains to human intestinal mucus and the role of adhesion in enteropathogen exclusion. | the ability of potential probiotic strains to adhere to the intestinal mucosa and exclude and displace pathogens is of utmost importance for therapeutic manipulation of the enteric microbiota. the ability of seven selected human bifidobacterial strains and five human enteropathogenic strains to adhere to human intestinal mucus was analyzed and compared with that of four strains isolated from chicken intestines. the adhesion of the bifidobacterial strains ranged from 3 to 16% depending on the str ... | 2005 | 16355841 |
| characteristics of listeria monocytogenes isolated from imported meat in japan. | the genomic structure of the iap region in listeria monocytogenes (serovar 4b), isolated from chicken imported into japan, was compared with those from japanese strains. the isolate was similar to the japanese strains in a comparatively new, rare group. such strains might be imported from foreign countries. | 2006 | 16495636 |
| virulence phenotyping and molecular characterization of a low-pathogenicity isolate of listeria monocytogenes from cow's milk. | a low-pathogenicity isolate of listeria monocytogenes from cow's milk, as screened in mouse and chicken embryonated egg models, was examined for virulence-related phenotypic traits. corresponding virulence genes (iap, prfa, plca, hly, mpl, acta, plcb, inla and inlb) were compared with l. monocytogenes reference strains 10403s and egd to elucidate the possible molecular mechanisms of low virulence. although l. monocytogenes h4 exhibited similar patterns to strain 10403s in terms of hemolytic acti ... | 2006 | 16604266 |
| thermal inactivation studies of escherichia coli o157:h7, salmonella, and listeria monocytogenes in ready-to-eat chicken-fried beef patties. | thermal inactivation studies were used to determine the d- and z-values of escherichia coli o157:h7, salmonella, and listeria monocytogenes in ready-to-eat chicken-fried beef patties. inoculated meat was packaged in sterile bags, which were immersed in a circulated water bath and held at 55, 57.5, 60, 62.5, 65, 67.5, and 70 degrees c for different lengths of time. d- and z-values were determined with a linear regression model. average d-values at temperatures 55 to 70 degrees c were 27.62 to 0.0 ... | 2006 | 16715808 |
| evaluation of aloa plating medium for its suitability to recover high pressure-injured listeria monocytogenes from ground chicken meat. | to evaluate a chromogenic plating medium for the isolation of sublethally injured cells of listeria monocytogenes from processed foods. | 2006 | 16910938 |
| [characterization of a recombinant listeria monocytogenes strain containing the fusion protein gene of newcastle disease virus]. | homologous recombination was utilized for construction of a recombinant strain of l. monocytogenes carrying a gene from the newcastle diseases virus by insertional mutation targeting its listeriolysin o gene (hly). the gene encoding fusion protein of the newcastle disease virus (ndv-f) was used as the model heterologous gene. the f gene was inserted into hly downstream to its promoter and signal sequence by overlapping extension polymerase chain reaction, which was then subcloned into the shuttl ... | 2006 | 16933618 |
| comparison of pathogenic and spoilage bacterial levels on refrigerated poultry parts following treatment with trisodium phosphate. | this study was undertaken to determine whether trisodium phosphate decontamination of poultry could give a competitive advantage to pathogens and increase microbiological risk to consumers. chicken legs were co-inoculated with similar concentrations of pathogenic (salmonella enteritidis or listeria monocytogenes) and spoilage (pseudomonas fluorescens or brochothrix thermosphacta) bacteria. samples were dipped in tsp (12%, 15 min) or were non-treated (control). microbiological analyses were carri ... | 2006 | 16943004 |
| investigation for possible source(s) of contamination of ready-to-eat meat products with listeria spp. and other pathogens in a meat processing plant in trinidad. | in 2003, there was a recall of three processed (chicken franks, spice ham and turkey ham ready-to-eat (rte) meat products by a large processing plant in trinidad as a result of contamination by listeria monocytogenes. the study was conducted to investigate the possible source(s) of listeria contamination of recalled rte meat products and to determine the prevalence of listeria spp., salmonella spp., escherichia coli and campylobacter spp. in the products and air within the plant. raw and process ... | 2006 | 16943025 |
| upstream sample processing facilitates pcr detection of listeria monocytogenes in mayonnaise-based ready-to-eat (rte) salads. | sample pretreatment to reduce volume and concentrate cells of the target organism(s) prior to molecular detection offers a useful supplement or alternative to cultural enrichment. the purpose of this study was to develop an upstream processing method to facilitate the detection of listeria monocytogenes in ready-to-eat (rte) salads by pcr. potato salad, a model rte commodity, was seeded with l. monocytogenes and processed by two alternative upstream sample processing methods (designated one-step ... | 2006 | 16943055 |
| efficacy of electrolyzed water in the inactivation of planktonic and biofilm listeria monocytogenes in the presence of organic matter. | the ability of electrolyzed (eo) water to inactivate listeria monocytogenes in suspension and biofilms on stainless steel in the presence of organic matter (sterile filtered chicken serum) was investigated. a five-strain mixture of l. monocytogenes was treated with deionized, alkaline eo, and acidic eo water containing chicken serum (0, 5, and 10 ml/liter) for 1 and 5 min. coupons containing l. monocytogenes biofilms were also overlaid with chicken serum (0, 2.5, 5.0, and 7.5 ml/liter) and then ... | 2006 | 16995516 |
| occurrence of pathogens in raw and ready-to-eat meat and poultry products collected from the retail marketplace in edmonton, alberta, canada. | a total of 800 meat and poultry products were purchased from the retail marketplace in edmonton, alberta, canada. the products consisted of raw ground beef, chicken legs, pork chops, and ready-to-eat fermented sausage, roast beef, processed turkey breast, chicken wieners, and beef wieners. the samples were analyzed to determine the prevalence of shiga toxin-producing escherichia coli, salmonella, campylobacter spp., and listeria monocytogenes. shiga toxin-producing e. coli 022: h8 was found in o ... | 2006 | 16995521 |
| characterization of listeria monocytogenes isolated from retail foods. | listeria monocytogenes isolates recovered from retail ready-to-eat (rte) meats, raw chickens and fresh produce were characterized by serogroup identification using pcr, genotyping using pulsed-field gel electrophoresis (pfge) and antimicrobial susceptibility testing. five l. monocytogenes serogroups were identified. of the 167 isolates 68 (41%) belonged to serogroup 1/2b, 3b; 53 (32%) belonged to serogroup 4b, 4d, 4e; 43 (26%) belonged to serogroup 1/2a, 3a; 2 (1.2%) belonged to serogroup 1/2c, ... | 2007 | 16996156 |
| the effect of lemon, orange and bergamot essential oils and their components on the survival of campylobacter jejuni, escherichia coli o157, listeria monocytogenes, bacillus cereus and staphylococcus aureus in vitro and in food systems. | to investigate the effectiveness of oils and vapours of lemon (citrus limon), sweet orange (citrus sinensis) and bergamot (citrus bergamia) and their components against a number of common foodborne pathogens. | 2006 | 17105553 |
| efficacy of lactic acid against listeria monocytogenes attached to poultry skin during refrigerated storage. | the aim of this study was to evaluate the effect of lactic acid washing on the growth of listeria monocytogenes on poultry legs stored at 4 degrees c for 7 days. | 2006 | 17105564 |
| microbial composition in bioaerosols of a high-throughput chicken-slaughtering facility. | the microbial composition of the air in various areas of a high-throughput chicken-slaughtering facility was investigated. over a 4-mo period, 6 processing areas were sampled, and the influence of environmental factors was monitored. the highest counts of microorganisms were recorded in the initial stages of processing, comprising the receiving-killing and defeathering areas, whereas counts decreased toward the evisceration, air-chilling, packaging, and dispatch areas. maximum microbial counts w ... | 2007 | 17179429 |
| microbiological baseline study of broiler chickens at swedish slaughterhouses. | this 1-year study was conducted to estimate the prevalence and concentrations of pathogenic and indicator bacteria on swedish broiler chickens. a total of 636 chilled carcasses were collected from 10 slaughterhouses and sent to the national food administration for analyses of carcass rinses. no carcasses were positive for salmonella. campylobacter, predominantly campylobacter jejuni, were detected on 15% (by enrichment) or 14% (by direct plating) of the carcasses. with one exception, all samples ... | 2006 | 17186653 |
| evaluation of effects of primary and secondary enrichment for the detection of listeria monocytogenes by real-time pcr in retail ground chicken meat. | a sybr green i based real-time pcr assay with inla-specific oligonucleotide primers was developed for easy and rapid detection of listeria monocytogenes in a model food that usually has a high incidence of contamination with this pathogen. results with pure cultures and artificially contaminated chicken meat samples indicate that the pcr assay was highly specific and sensitive. the melting point analysis of the 160 bp amplified dna fragment was different for l. monocytogenes isolates of the two ... | 2006 | 17199516 |
| listeria monocytogenes mutants carrying newcastle disease virus f gene fused to its acta and plcb: in vitro expression and immunogenicity in chickens. | recombinant listeria monocytogenes mutants carrying newcastle disease virus (ndv) fusion protein gene f were constructed by homologous recombination. ndv f or its truncated fragment fa was used as the model heterologous gene to be integrated into acta or plcb downstream of their signal sequences. correct orientation of the inserted genes was verified by polymerase chain reaction amplification of f or fa. the inserted f and fa were expressed in the two recombinants lm-deltaacta-f and lm-deltaplcb ... | 2007 | 17213959 |
| isolation and molecular characterization of antibiotic-resistant lactic acid bacteria from poultry and swine meat products. | the transfer via the food chain from animals to humans of microbes that are resistant to antimicrobial agents is of increasing concern. to determine the contributions of nonpathogenic microflora to the occurrence and spread of antibiotic resistance (ar) genes in the food chain, 123 lactic acid bacteria were isolated from 29 samples of raw and processed pork and chicken meat products that had previously tested positive for one or more ar genes that encode clinically relevant ars: tet(m), tet(o), ... | 2007 | 17388042 |
| quantitative effect of refrigerated storage time on the enumeration of campylobacter, listeria, and salmonella on artificially inoculated raw chicken meat. | active monitoring of pathogens on retail foods has been recommended and implemented in a number of developed countries. because only a portion of retail food is contaminated with pathogens, a cost-effective and informative surveillance program at the retail level often involves a two-stage approach of initial presence-absence analysis and subsequent pathogen enumeration in any positive samples. most-probable-number (mpn) methods are more resource intensive and therefore used only for samples con ... | 2007 | 17388068 |
| canine cathelicidin (k9cath): gene cloning, expression, and biochemical activity of a novel pro-myeloid antimicrobial peptide. | cathelicidins, a group of cationic peptides found in leukocytes and epithelial cells, play a central role in the early innate immune defense against infection. although these host defense peptides have been reported in several mammalian species, including primates, no cathelicidins have been identified in carnivores. here we report the cloning, tissue expression and biological activity of a novel canine cathelicidin (k9cath). the full-length cdna sequence of k9cath encodes a predicted 172 amino ... | 2007 | 17462733 |
| microbiological safety of retail vacuum-packed and modified-atmosphere-packed cooked meats at end of shelf life. | a study of retail modified-atmosphere-packed and vacuum-packed cooked ready-to-eat meats was undertaken from september through mid-november 2003 to determine the microbiological quality at the end of shelf life and to establish any risk factors in the production, storage, and display of this product. examination of 2,981 samples using microbiological guidelines criteria revealed that 66% were of satisfactory or acceptable microbiology quality, 33% were of unsatisfactory quality mainly due to hig ... | 2007 | 17477265 |
| inhibition of listeria monocytogenes using nisin with grape seed extract on turkey frankfurters stored at 4 and 10 degrees c. | recontamination of cooked ready-to-eat (rte) chicken and beef products with listeria monocytogenes has been a major safety concern. natural antimicrobials in combinations can be an alternative approach for controlling l. monocytogenes. therefore, the objectives of this study were to evaluate the inhibitory activities against l. monocytogenes of nisin (6,400 iu/ ml), grape seed extract (gse; 1%), and the combination of nisin and gse both in tryptic soy broth with 0.6% yeast extract (tsbye) and on ... | 2007 | 17477277 |
| evaluation in vitro of the antagonistic substances produced by lactobacillus spp. isolated from chickens. | to determine the inhibitory capacity of lactic acid bacteria due to the action of antagonistic substances, we tested 474 isolates of lactobacillus from the crop and cecum of chickens against gram-positive and gram-negative indicator microorganisms by the spot-on-the-lawn and well-diffusion antagonism methods. of the 474 isolates, 265 demonstrated antimicrobial activity against the indicator microorganisms. isolates identified as l. reuteri, l. salivarius, or lactobacillus spp. inhibited enteroco ... | 2007 | 17479773 |
| modification of chicken avian beta-defensin-8 at positively selected amino acid sites enhances specific antimicrobial activity. | antimicrobial peptides (amps), essential components of innate immunity, are found in a range of phylogenetically diverse species and are thought to act by disrupting the membrane integrity of microbes. in this paper, we used evolutionary signatures to identify sites that are most relevant during the functional evolution of these molecules and introduced amino acid substitutions to improve activity. we first demonstrate that the anti-microbial activity of chicken avian beta-defensin-8, previously ... | 2007 | 17483936 |
| traceback identification of an ingredient (pork dewlap) as the possible source of listeria monocytogenes serotype 4b contamination in raw chicken products. | in surveys conducted on finished product samples from a single poultry processing plant in spain, listeria monocytogenes was found in 14 different uncooked products. to track contamination patterns, 77 l. monocytogenes isolates were characterized by pcr-based serotyping, pulsed-field gel electrophoresis (pfge) restriction analysis, and pcr-based allelic analysis of the virulence gene acta. serotyping revealed that 12 isolates (15.6%) were of the l. monocytogenes serotype 4b complex (serotype 4b ... | 2007 | 17612086 |
| aerosol studies with listeria innocua and listeria monocytogenes. | aerosol studies of listeria monocytogenes in food processing plants have been limited by lack of a suitable surrogate microorganism. the objective of this study was to investigate the potential of using green fluorescent protein-labeled strains of listeria innocua as a surrogate for l. monocytogenes for aerosol studies. these studies were conducted in a laboratory bioaerosol chamber and a pilot food-processing facility. four strains of l. innocua and five strains of l. monocytogenes were used. i ... | 2007 | 17803142 |
| in vitro study on bacteriocin production of enterococci associated with chickens. | in recent years, the approach of using innovative strategies such as probiotics or bacteriocins for the prevention or treatment of bacterial infections has come into focus. the present study was undertaken to check in vitro ability of enterococci-isolated from the gastrointestinal tract of chickens-to produce a bacteriocin-like substance and to describe some further probiotic properties in five selected enterococcus faecium strains. all strains (n=17) were found to produce bacteriocin-like subst ... | 2007 | 17884622 |
| effectiveness of trisodium phosphate, acidified sodium chlorite, citric acid, and peroxyacids against pathogenic bacteria on poultry during refrigerated storage. | the effects of dipping treatments (15 min) in potable water or in solutions (wt/vol) of 12% trisodium phosphate (tsp), 1,200 ppm acidified sodium chlorite (asc), 2% citric acid (ca), and 220 ppm peroxyacids (pa) on inoculated pathogenic bacteria (listeria monocytogenes, staphylococcus aureus, bacillus cereus, salmonella enteritidis, escherichia coli, and yersinia enterocolitica) and skin ph were investigated throughout storage of chicken legs (days 0, 1, 3, and 5) at 3 +/- 1 degrees c. all chemi ... | 2007 | 17900083 |
| a 2-step cooking method of searing and hot water pasteurization to maximize the safety of refrigerated, vacuum packaged, chicken breast meat. | americans consume almost 40 kg per capita of chicken each year. increasing consumption of chicken surpassed pork in 1982 and beef in 1992. the objectives of this study were to examine the effectiveness of a novel, 2-step cooking method of grilling, slicing, vacuum packaging, and hot water pasteurization to inhibit the growth of listeria monocytogenes in chicken breast meat. because this study required the use of pilot plant scale pasteurization equipment, listeria innocua m1, a nonpathogen with ... | 2007 | 17995778 |
| effects of essential oils of oregano and nutmeg on growth and survival of yersinia enterocolitica and listeria monocytogenes in barbecued chicken. | the in vitro effects of plant essential oils (eos) against pathogenic bacteria are well known, yet few studies have addressed the effects of these compounds against pathogens associated with ready-to-cook foods. experiments were conducted to determine the effectiveness of oregano and nutmeg eos on the growth and survival of yersinia enterocolitica and listeria monocytogenes in broth culture and in iranian barbecued chicken. ready-to-cook iranian barbecued chicken was prepared according to the co ... | 2007 | 18044446 |
| isolation and purification of enterocin e-760 with broad antimicrobial activity against gram-positive and gram-negative bacteria. | strain nrrl b-30745, isolated from chicken ceca and identified as enterococcus durans, enterococcus faecium, or enterococcus hirae, was initially identified as antagonistic to campylobacter jejuni. the isolate produced a 5,362-da bacteriocin (enterocin) that inhibits the growth of salmonella enterica serovar enteritidis, s. enterica serovar choleraesuis, s. enterica serovar typhimurium, s. enterica serovar gallinarum, escherichia coli o157:h7, yersinia enterocolitica, citrobacter freundii, klebs ... | 2008 | 18086839 |
| different enrichment procedures for recovery of listeria monocytogenes from raw chicken samples can affect the results of detection (by chromogenic plating or real-time pcr) and lineage or strain identification. | this study aimed to evaluate the effect of different enrichment procedures on the detection of listeria monocytogenes in food, by a comparison of subculture onto chromogenic agar with real-time pcr. two different culture media, the primary and secondary enrichment broths of the u.s. food safety and inspection service (fsis) method used for pcr detection of l. monocytogenes, were compared for the primary enrichment of retail ground chicken samples. l. monocytogenes was detected after the completi ... | 2007 | 18095442 |
| application of pcr-sscp for molecular epidemiological studies on the exposure of farm children to bacteria in environmental dust. | the environmental exposure of farm children to microorganisms in dust has become a focus of interest, since microbial exposure on farms has been related to a reduced prevalence of asthma and atopic diseases in children. previous studies almost exclusively focused on the determination of microbial counts using conventional culturing or the determination of microbial compounds i.e. endotoxins. in this study pcr-sscp (single-strand conformation polymorphism) was modified for characterising bacteria ... | 2008 | 18321601 |
| enhanced rapidity for qualitative detection of listeria monocytogenes using an enzyme-linked immunosorbent assay and immunochromatography strip test combined with immunomagnetic bead separation. | an enzyme-linked immunosorbent assay (elisa), immunochromatography (icg) strip test, and immunomagnetic bead separation (imbs) system based on a monoclonal antibody were individually developed for the detection and isolation of listeria monocytogenes in meat samples. the three methods showed a strong reaction with listeria species and a weak reaction with staphylococcus aureus. to increase the rapidity of l. monocytogenes detection, combinations of the elisa and icg strip test with the imbs syst ... | 2008 | 18468033 |
| prevalence of listeria monocytogenes and salmonella in ready-to-eat food in catalonia, spain. | listeria monocytogenes and salmonella are pathogenic bacteria that can contaminate food products during or after processing. ready-to-eat (rte) food does not undergo any treatment to ensure its safety before consumption, and therefore risk of foodborne disease must be considered if these pathogens are present in the food. to evaluate the prevalence of these pathogens in rte food, 140 rte fish product samples, 501 rte meat product samples, 462 rte dairy samples, and 123 rte dishes and desserts, p ... | 2008 | 18468047 |
| the effect of an acidic, copper sulfate-based commercial sanitizer on indicator, pathogenic, and spoilage bacteria associated with broiler chicken carcasses when applied at various intervention points during poultry processing. | studies were conducted to evaluate 1) the effect of an acidic, copper sulfate-based commercial sanitizer on pathogenic, indicator, and spoilage bacteria in a model scalder system, 2) the effect of this sanitizer on total aerobic bacteria (apc) and escherichia coli counts, and salmonella prevalence on broiler chicken carcasses when applied during scalding or scalding and postpick dipping, and 3) the ability of sanitizer to extend the shelf-life of broiler chicken carcasses. exposure of salmonella ... | 2008 | 18577627 |
| [molecular typing of listeria monocytogenes isolated from foodstuff in guangdong province by pulsed-field gel electrophoresis]. | to establish molecular typing of listeria monocytogenes isolates by pulsed-field gel electrophoresis (pfge) for studying the epidemiologic characteristics of listeria monocytogenes isolated from foodstuff in guangdong province and to build up pfge typing database of listeria monocytogenes isolates for identifying the infectious resource of the outbreaks and other epidemiologic investigation. | 2008 | 18785476 |
| comparative analysis of extracellular and intracellular proteomes of listeria monocytogenes strains reveals a correlation between protein expression and serovar. | listeria monocytogenes, the etiologic agent of listeriosis, remains a serious public health concern, with its frequent occurrence in food environments coupled with a high mortality rate. among the 13 serovars, human listeriosis is mostly associated with the serovar 4b, 1/2b, and 1/2a strains. to investigate the diversity of l. monocytogenes, the intracellular and extracellular proteins of 12 strains were analyzed by two-dimensional gel electrophoresis. these strains had different origins, belong ... | 2008 | 18836007 |
| isolation and characterization of bacteriocin-producing bacteria from the gastrointestinal tract of broiler chickens for probiotic use. | to isolate and characterize the bacteriocin-producing bacteria (bpb) from the gastrointestinal tract of broiler chickens for probiotic use. | 2008 | 19120666 |
| anti-listerial activity of bacteriocin-producing lactobacillus curvatus cwbi-b28 and lactobacillus sakei cwbi-b1365 on raw beef and poultry meat. | the study aimed to evaluate the effect of the bacteriocins produced by lactobacillus sakei cwbi-b1365 and lactobacillus curvatus cwbi-b28 on the growth and survival of listeria monocytogenes in raw beef and poultry meat. | 2008 | 19120930 |
| occurrence and distribution of listeria monocytogenes and other listeria species in ready-to-eat and raw meat products. | the present study was undertaken to estimate the occurrence and distribution of listeria monocytogenes and other listeria species in ready-to-eat food items (pasteurized milk, cheese, ice cream, and cakes) and raw meat products (minced beef, pork, and chicken carcasses). a total of 711 randomly selected samples were collected from supermarkets and pastry shops in addis ababa, ethiopia. listeria monocytogenes and other listeria species were isolated and identified according to the techniques reco ... | 2009 | 19226932 |
| listeriosis. | listeria monocytogenes, a small, facultative anaerobic, gram-positive motile bacillus, is an important cause of foodborne illness which disproportionately affects pregnant women and their newborns. listeria infects many types of animals and contaminates numerous foods including vegetables, milk, chicken and beef. this organism has a unique proclivity to infect the fetoplacental unit with the ability to invade cells, multiply intracellularly and be transmitted cell-to-cell. the organism possesses ... | 2009 | 19231307 |
| functional properties of novel protective lactic acid bacteria and application in raw chicken meat against listeria monocytogenes and salmonella enteritidis. | in this study 635 lactic acid bacteria of food origin were evaluated for their potential application as protective cultures in foods. a stepwise selection method was used to obtain the most appropriate strains for application as protective cultures in chicken meat. specifically, all strains were examined for antimicrobial activity against various gram positive and gram negative pathogenic and spoilage bacteria. strains exhibiting anti-bacterial activity were subsequently examined for survival in ... | 2009 | 19249112 |
| differences in pressure tolerance of listeria monocytogenes strains are not correlated with other stress tolerances and are not based on differences in ctsr. | thirty strains of listeria monocytogenes were screened for their pressure tolerance phenotype at 400 mpa for 2 min at 21 degrees c. the strains exhibited reductions ranging from 1.9 to 7.1 log(10)cfu/ml in tryptic soy broth with 6% yeast extract (tsbye). the 3 most and the 3 least pressure-tolerant strains were further tested for their thermal resistance (based on their ability to survive at 55 degrees c), acid tolerance (based on their ability to survive in acidified tsbye; ph 2.0) and for thei ... | 2009 | 19376462 |
| broad range evaluation of the matrix solubilization (matrix lysis) strategy for direct enumeration of foodborne pathogens by nucleic acids technologies. | a previously published rapid (<5 h) proof-of-concept protocol for the concentration of the foodborne pathogen listeria monocytogenes from milk, based on the solubilization of the food matrix, was further evaluated. the original protocol was modified to detect gram-negative and other gram-positive bacteria and to broaden the range of food matrices by using lutensol instead of sodium dodecyl sulfate as the main detergent in the buffer. a new protocol using a protease and sucrose buffer was establi ... | 2009 | 19610333 |
| a multifactorial risk prioritization framework for foodborne pathogens. | we develop a prioritization framework for foodborne risks that considers public health impact as well as three other factors (market impact, consumer risk acceptance and perception, and social sensitivity). canadian case studies are presented for six pathogen-food combinations: campylobacter spp. in chicken; salmonella spp. in chicken and spinach; escherichia coli o157 in spinach and beef; and listeria monocytogenes in ready-to-eat meats. public health impact is measured by disability-adjusted l ... | 2010 | 19671103 |
| inactivation of listeria monocytogenes on unpackaged and vacuum-packaged chicken frankfurters using pulsed uv-light. | the effectiveness of pulsed uv-light on the microbial load and quality of unpackaged and vacuum-packaged chicken frankfurters was investigated. samples were inoculated with listeria monocytogenes scott a on the top surfaces, and then treated with pulsed uv-light for 5, 15, 30, 45, and 60 s at 5, 8, and 13 cm distance from the quartz window in a pulsed uv-light chamber. log reductions (cfu/cm(2)) on unpackaged samples were between 0.3 and 1.9 after 5-s treatment at 13 cm and 60-s treatment at 5 c ... | 2009 | 19799670 |
| edible apple film wraps containing plant antimicrobials inactivate foodborne pathogens on meat and poultry products. | apple-based edible films containing plant antimicrobials were evaluated for their activity against pathogenic bacteria on meat and poultry products. salmonella enterica or e. coli o157:h7 (10(7) cfu/g) cultures were surface inoculated on chicken breasts and listeria monocytogenes (10(6) cfu/g) on ham. the inoculated products were then wrapped with edible films containing 3 concentrations (0.5%, 1.5%, and 3%) of cinnamaldehyde or carvacrol. following incubation at either 23 or 4 degrees c for 72 ... | 2009 | 19799671 |
| chicken salad as the source of a case of listeria monocytogenes infection in connecticut. | listeriosis is a severe infection with high morbidity and mortality. we report a fatal case of listeriosis in a patient with a history of crohn's disease who consumed chicken salad purchased from a retail food establishment before developing listeriosis. as part of the regulatory testing programs, the u.s. department of agriculture food safety and inspection service and the florida department of agriculture and consumer affairs found that chicken products from a single food-production establishm ... | 2009 | 20003746 |
| applicability of biological time temperature integrators as quality and safety indicators for meat products. | the objective of this study was to evaluate (eo), a biological time temperature integrator (tti) as a quality and safety indicator for ground beef packed under modified atmosphere and spiced cooked chicken slices packed under modified atmosphere. storage trials and challenge tests were thus performed on several batches of the studied food to monitor and model the behavior of listeria monocytogenes, salmonella, staphylococcus aureus and the indigenous food flora. then, two different prototypes of ... | 2010 | 20074826 |
| a multiplex real-time polymerase chain reaction for simultaneous detection of salmonella spp., escherichia coli o157, and listeria monocytogenes in meat products. | to achieve an effective detection of salmonella spp., escherichia coli o157, and listeria monocytogenes in meat products, a multiplex real-time polymerase chain reaction (pcr) coupled with a multipathogen enrichment strategy was developed in this study. pathogen-specific dna sequences in the inva, rfbe, and hlya genes were employed to design primers and taqman probes for identifying salmonella spp., e. coli o157, and l. monocytogenes, respectively. an internal amplification control (iac) utilizi ... | 2010 | 20113204 |
| colonization of a newly constructed commercial chicken further processing plant with listeria monocytogenes. | this study was undertaken to determine potential sources of listeria monocytogenes in a newly constructed chicken further processing plant and document the eventual colonization of the facility by this pathogen. to ascertain the colonization status of the plant, floor drains were sampled after a production shift and again after a cleanup shift on roughly a monthly basis for 21 months. potential sources of l. monocytogenes to the plant included incoming raw meat, incoming fresh air, and personnel ... | 2010 | 20132673 |
| effect of high pressure on the microbiological quality of cooked chicken during storage at normal and abuse refrigeration temperatures. | vacuum-packaged cooked poultry meat was treated at a range of pressures (400-600 mpa) and hold times (1, 2 and 10 min), followed by storage at 4 degrees , 8 degrees or 12 degrees c for up to 35 days. weissella viridescens was found to be the dominant microorganism in the pressure-treated meat, constituting 100% of the microflora identified at 500 and 600 mpa. none of the pressure-treated samples had obvious signs of spoilage during the 35 day storage period, even when the weissella count was >7 ... | 2010 | 20141945 |
| antibody-aptamer functionalized fibre-optic biosensor for specific detection of listeria monocytogenes from food. | to develop antibody-aptamer functionalized fibre-optic biosensor for specific detection of listeria monocytogenes from food products. | 2010 | 20337767 |
| experimental antibacterial therapy with puroindolines, lactoferrin and lysozyme in listeria monocytogenes-infected mice. | puroindoline a and puroindoline b from plant seeds, bovine lactoferrin and chicken eggs lysozyme are antimicrobial proteins of innate immune system that lyse invading organisms. we investigate their potential antibacterial activity against listeria monocytogenes in a mouse model. bacteria were isolated from various organs for 7 days after challenge. livers displayed consistently higher bacterial count (up to 10(7)cfu/g) than spleens, kidneys and brains. the efficacy of the amps was therefore est ... | 2010 | 20348006 |
| effect of organic acids and plant extracts on escherichia coli o157:h7, listeria monocytogenes, and salmonella typhimurium in broth culture model and chicken meat systems. | foodborne illness due to consumption of products contaminated with salmonella typhimurium (s.t.), listeria monocytogenes (l.m.), and escherichia coli o157:h7 (e.c.) results in many deaths and significant economic losses each year. in this study, acetic (aa), citric acid (ca), lactic acid (la), malic acid (ma), and tartaric acid (ta) and grape seed (gs), green tea (gt), bitter melon seed (bms), rasum, and fenugreek (fg) extracts were investigated as inhibitors against s.t., l.m., and e.c. in both ... | 2009 | 20492123 |
| active packaging of fresh chicken breast, with allyl isothiocyanate (aitc) in combination with modified atmosphere packaging (map) to control the growth of pathogens. | listeria monocytogenes and salmonella typhimurium are major bacterial pathogens associated with poultry products. ally isothiocyanate (aitc), a natural antimicrobial compound, is reportedly effective against these pathogenic organisms. a device was designed for the controlled release of aitc with modified atmosphere packaging (map), and then evaluated for its ability to control the growth of l. monocytogenes and s. typhimurium on raw chicken breast during refrigerated storage. in order to obtain ... | 2010 | 20492243 |
| a multipathogen selective enrichment broth for simultaneous growth of salmonella enterica serovar enteritidis, staphylococcus aureus, and listeria monocytogenes. | a selective enrichment broth (ssl) was formulated to allow concurrent growth of 3 prominent food-borne pathogens: salmonella enterica serovar enteritidis, staphylococcus aureus, and listeria monocytogenes. nalidixic acid, lithium chloride, and potassium tellurite were added as the selective agents, while sodium pyruvate and mannitol were employed as the supplemented elements. in the individual growth trial, the target pathogens were capable of growing in ssl to as high as 7-8 log(10) colony-form ... | 2010 | 20651858 |
| presence and incidence of food-borne pathogens in australian chicken litter. | 1. litter samples were collected at the end of the production cycle from spread litter in a single shed from each of 28 farms distributed across the three eastern seaboard states of australia. 2. the geometric mean for salmonella was 44 most probable number (mpn)/g for the 20 positive samples. five samples were between 100 and 1000 mpn/g and one at 10(5) mpn/g, indicating a range of factors are contributing to these varying loads of this organism in litter. 3. the geometric mean for campylobacte ... | 2010 | 20680865 |
| partial purification and characterization of the mode of action of enterocin s37: a bacteriocin produced by enterococcus faecalis s37 isolated from poultry feces. | the aim of this research was to purify and characterize the mode of action of enterocin s37, a bacteriocin produced by enterococcus faecalis s37, a strain recently isolated from the chicken feces. enterocin s37 has a molecular weight comprised between 4 and 5 kda. it remained active after 1 h at 80(o)c and at ph values ranging from 4.0 to 9.0. furthermore, cell-free supernatant of enterococcus faecalis s37 and purified enterocin s37 were active against gram-positive bacteria including listeria m ... | 2010 | 20811593 |
| prevalence of listeria monocytogenes in ready-to-eat foods sampled from the point of sale in wales, united kingdom. | a survey of listeria in ready-to-eat food took place in wales, united kingdom, between february 2008 and january 2009. in total, 5,840 samples were taken and examined for the presence of listeria species, including l. monocytogenes. samples were tested using detection and enumeration methods, and the results were compared with current united kingdom guidelines for the microbiological quality of ready-to-eat foods. the majority of samples were negative for listeria by both direct plating and enri ... | 2010 | 20819364 |
| prevalence of listeria monocytogenes in retailed meat in the tokyo metropolitan area. | this study was conducted to determine the prevalence of listeria monocytogenes in retailed meats, comprising beef, chicken, and pork, in the tokyo metropolitan area. a total of 379 samples of retailed meat were collected from 1998 to 2003, most of which were obtained by simultaneously purchasing the three classes of meat from a shop and then making another simultaneous purchase of meat from the same shop a few weeks later. the prevalence of l. monocytogenes was 28.0%, and the serotypes isolated ... | 2010 | 20828477 |
| inactivation of cronobacter sakazakii by ultrasonic waves under pressure in buffer and foods. | the objective of this research was to characterize the resistance of cronobacter sakazakii to ultrasonic waves under pressure (manosonication, ms). the d(ms) value (decimal reduction time value) of c. sakazakii in standard conditions (35°c, 117 μm, 200 kpa, citrate-phosphate buffer ph 7.0) was 0.41 min. this value was higher than that of yersinia enterocolitica (d(ms)=0.19 min) and lower than those of salmonella enterica serovar enteritidis (d(ms)=0.61 min), listeria monocytogenes (d(ms)=0.86 mi ... | 2010 | 21111503 |
| risk factors for listeria monocytogenes contamination in french laying hens and broiler flocks. | the objective of this study was to identify potential risk factors for listeria monocytogenes contamination in french poultry production. eighty-four flocks of layer hens kept in cages and 142 broiler flocks were included in this study. for each production type, a questionnaire was submitted to farmers and fecal samples were taken to assess the l. monocytogenes status of the flocks during a single visit to the farm. two logistic regression models (specific to each production) were used to assess ... | 2010 | 21176855 |
| the inhibitory effect of natural microflora of food on growth of listeria monocytogenes in enrichment broths. | the aims of this study were to (i) compare the inhibitory effects of the natural microflora of different foods on the growth of listeria monocytogenes during enrichment in selective and non-selective broths; (ii) to isolate and identify components of the microflora of the most inhibitory food; and (iii) to determine which of these components was most inhibitory to growth of l. monocytogenes in co-culture studies. growth of an antibiotic-resistant marker strain of l. monocytogenes was examined du ... | 2010 | 21176988 |
| pathogenicity and immunogenicity of a mutant strain of listeria monocytogenes in the chicken infection model. | listeria monocytogenes has been exploited as a vaccine carrier based upon its ability to induce a strong cell-mediated immune response. at present, the safety of live, attenuated l. monocytogenes vaccines in patients is being studied in clinical trials. l. monocytogenes is also an attractive vaccine vector for use in poultry; however, the pathogenicity and immunogenicity of this organism in poultry remain to be fully elucidated. in this study, we investigated the pathogenicity and immunogenicity ... | 2011 | 21228136 |
| efficacy of washing meat surfaces with 2% levulinic, acetic, or lactic acid for pathogen decontamination and residual growth inhibition. | we compared spray washing at 55.4 °c with 2% levulinic acid to that with lactic or acetic acid for decontamination of pathogenic bacteria inoculated onto meat surfaces, and their residual protection against later growth of pathogenic bacteria. the model systems included escherichia coli o157:h7 on beef plate, salmonella on chicken skin and pork belly, and listeria monocytogenes on turkey roll. in the decontamination studies, acid washes lowered recoverable numbers of pathogens by 0.6 to 1 log/cm ... | 2010 | 21251765 |
| assessment of the antibacterial activity of a triclosan-containing cutting board. | several studies have shown that consumers may not clean cutting boards properly between preparation of raw and cooked meat. cutting boards may therefore act as sources for contamination of cooked meat or other ready-to-eat foods with pathogenic and spoilage bacteria. the aim of the work was to investigate if cutting boards containing the antimicrobial compound triclosan can reduce the viability of bacteria, thus acting as a hygiene barrier. survival and growth of food pathogens and spoilage bact ... | 2011 | 21402423 |
| antibacterial activity of egg yolk antibody (igy) against listeria monocytogenes and preliminary evaluation of its potential for food preservation. | background: egg yolk antibody (igy) is a unique type of immunoglobulin found in egg yolks, and many reports have described its ability to inhibit corresponding antigen bacteria. the objective of this study was to evaluate the antibacterial activity of igy specific to listeria monocytogenes, an important food pathogen to both humans and animals, as well as its potential use for food preservation. results: specific igy was generated by immunising leghorn chickens with whole cells of l. monocytogen ... | 2011 | 21495036 |
| risk evaluation and management to reaching a suggested fso in a steam meal. | steam meals are ready-to-eat meals composed of raw and semi-cooked ingredients, which get cooked while microwave heating. in this study, an indian style meal was selected, chicken tandoori, from two different producers. these meals were first evaluated with the risk ranger® to identify the main foodborne pathogens risks, which were listeria monocytogenes, salmonella typhimurium and bacillus cereus. thereafter, quantitative microbiology was applied using different models and verified with growth ... | 2011 | 21511122 |
| antilisterial activity and consumer acceptance of irradiated chicken breast meat vacuum-infused with grape seed and green tea extracts and tartaric acid. | contamination of poultry with pathogenic bacteria contributes to human foodborne disease, causes damage to industry brand names, and has a significant economic impact on the food industry in the form of both damage to industry brand names and losses associated with recalls. irradiation is a safe and effective means of decontaminating poultry products, but the maximum dose strengths allowed negatively impact poultry sensory quality characteristics. the 1st objective of this study was to investiga ... | 2010 | 21535556 |
| multiple-locus variable number of tandem repeat analysis (mlva) of listeria monocytogenes directly in food samples. | listeria monocytogenes is the etiologic agent of listeriosis responsible for severe and fatal infections in humans. listeria contamination occurs quite often in a wide range of foods due to its ubiquitous nature. isolates need to be characterized to a strain level for accurate diagnosis of listeria infection, epidemiological studies, investigation of outbreaks and effective prevention and control of food-borne listeriosis. the purpose of this research was to evaluate the multiple-locus variable ... | 2011 | 21550679 |
| prevalence and antimicrobial resistance of listeria monocytogenes isolated in chicken slaughterhouses in northern greece. | this study was conducted to determine the prevalence and antimicrobial resistance of listeria monocytogenes recovered from chicken carcasses in slaughterhouses in northern greece. a total of 100 poultry samples (300 carcasses) were examined for listeria spp. the samples were neck skin taken from four different slaughterhouses in northern greece. forty samples were also taken from the environment of the slaughterhouses. identification of l. monocytogenes was carried out by pcr and fingerprinting ... | 2011 | 21669084 |
| microbial challenges of poultry meat production. | food safety and shelf-life are both important microbial concerns in relation to broiler meat production. focus is mainly placed on the absence or control of potentially pathogenic microbes such as salmonella spp. and campylobacter spp. but, from the commercial point of view, other spoilage bacteria also play a role as potential threats. regarding food safety, the primary target should be the production of pathogen-free live animals, thus allowing slaughter plants to keep the processing line free ... | 2011 | 21718794 |
| effect of lactoferrin and its derivatives against gram-positive bacteria in vitro and, combined with high pressure, in chicken breast fillets. | the bactericidal activity of lactoferrin (lf), amidated lactoferrin (amilf), pepsin digested lactoferrin (pdlf), and its activated (alf) commercial form, against six strains of three gram-positive bacterial species was investigated. listeria monocytogenes was most sensitive in vitro, staphylococcus aureus showed a moderate resistance, and enterococus faecalis was highly resistant to antimicrobials. when chicken breast fillets were inoculated with l. monocytogenes cect5725 and treated with antimi ... | 2011 | 21703778 |
| prevalence of foodborne pathogens in grilled chicken from street vendors and retail outlets in reynosa, tamaulipas, mexico. | we analyzed a total of 70 grilled chicken samples bought randomly from street vendors and retail outlets in the city of reynosa, mexico, to determine the prevalence of escherichia coli (shiga toxin producing and enterotoxin producing), salmonella spp., staphylococcus aureus, listeria spp., and campylobacter spp. using microbiological methods and pcr detection of bacterial sequences. of the 70 samples, 27 (38.5%) were from retail outlets and 43 (61.4%) from street vendors. all specimens were nega ... | 2011 | 21819659 |
| a predictive model for the inactivation of listeria innocua in cooked poultry products during postpackage pasteurization. | contamination of listeria monocytogenes in ready-to-eat poultry products poses potential risk of listeriosis to the public. to control the level of listeria contamination, attention has been focused on the postpackage pasteurization of fully cooked poultry products. in this study, we sought to develop a model to predict the thermal inactivation of l. monocytogenes in chicken drumettes during postpackage hot water pasteurization. fully cooked chicken drumettes were inoculated with listeria innocu ... | 2011 | 21819652 |
| lactic acid bacteria from chicken carcasses with inhibitory activity against salmonella spp. and listeria monocytogenes. | this study was conducted to isolate psychrotrophic lactic acid bacteria (lab) from chicken carcasses with inhibitory activity against strains of salmonella spp. and listeria monocytogenes. a total of 100 broiler samples were examined for the presence of lab. ninety-two lab isolates that showed antimicrobial effects against salmonella spp. and l. monocytogenes were further analysed to examine their lab (gram-positive, catalase negative, oxidase negative) and psychrotrophic characteristics (abilit ... | 2011 | 21983108 |
| inhibition of listeria monocytogenes and salmonella enteritidis on chicken wings using scallop-shell powder. | the present study was designed to determine the inhibitory effect of scallop-shell powder (ssp) on listeria monocytogenes and salmonella enteritidis on chicken wings. chicken wings artificially inoculated with l. monocytogenes (8.3 log(10) cfu/g) or s. enteritidis (8.8 log(10) cfu/g) were immersed in distilled water (0.10 or 0.50% wt/vol) of ssp slurries for 10 or 30 min, respectively. the amount of l. monocytogenes, s. enteritidis, mesophilic aerobic bacteria (mab), and yeast or mold was determ ... | 2011 | 22010247 |
| rapid and sensitive detection of listeria monocytogenes by loop-mediated isothermal amplification. | loop-mediated isothermal amplification (lamp) was designed for detection of listeria monocytogenes, which is an important food-borne kind of pathogenic bacteria causing human and animal disease. the primers set for the hlya gene consist of six primers targeting eight regions on specific gene. the lamp assay could be performed within 40 min at 65°c in a water bath. amplification products were visualized by calcein and manganous ion and agarose gel electrophoresis. sensitivity of the lamp assay fo ... | 2011 | 21935669 |