| antibodies against human cmv-induced "early" antigens detected by immunoblotting are not exclusively associated with active infection. | we investigated the correlation between the prevalence of antibodies against human cytomegalovirus (hcmv) "early" proteins and the state of infection. two hundred and twenty-eight hcmv-igg-positive (hcmv late-antigen elisa) sera, drawn from subjects with active and non-active hcmv infections, were tested for specific igg antibodies against hcmv "early" antigens by a western blotting micromethod. applying virus strain ad-169, we found antibodies to hcmv "early" antigens in 59% of all cases, irres ... | 1991 | 1647051 |
| sensitive non-isotopic dna hybridisation assay or immediate-early antigen detection for rapid identification of human cytomegalovirus in urine. | a sensitive non-radioactive dna hybridisation assay employing digoxigenin-labelled probes was compared with immediate-early antigen detection and conventional virus isolation for the identification of human cytomegalovirus (hcmv) in 249 urine samples. of 44 specimens yielding hcmv by virus isolation, more were positive by dna hybridisation (32; 73%) than by immediate-early antigen detection (25; 52%) (p = 0.05). the specificity of the hybridisation assay in 45 apparently falsely positive specime ... | 1991 | 1648575 |
| value of animal models to evaluate agents with potential activity against human cytomegalovirus. | | 1991 | 1648821 |
| ganciclovir-resistant human cytomegalovirus clinical isolates; resistance mechanisms and in vitro susceptibility to antiviral agents. | | 1991 | 1648824 |
| the procoagulant response of cytomegalovirus infected endothelial cells. | the report describes the effect of an in vitro infection of human umbilical vein endothelial cells with human cytomegalovirus (cmv). the parameters studied are cellular procoagulant activity, secretion of plasminogen activator inhibitor (pai-1) and urokinase-type plasminogen activator (u-pa), activation and internalization of factor x and merocyanine 540 staining. the infection does not result in an increase in pai-1 and u-pa secretion, but it brings about a procoagulant response, which is relat ... | 1992 | 1332211 |
| identification of the human herpesvirus 6 glycoprotein h and putative large tegument protein genes. | determination of the nucleotide sequences of two molecular clones of human herpesvirus 6 (hhv-6) (strain gs) and comparison with those of human cytomegalovirus (hcmv) has allowed the identification of the genes for the glycoprotein h (gh) and the putative large tegument protein of hhv-6. two molecular clones of fragments of hhv-6, the bamhi-g fragment (7,981 bp) of the clone termed pzvb43 and a hindiii fragment (8,717 bp) of the clone termed pzvh14, represent approximately 10% of the hhv-6 genom ... | 1991 | 1654455 |
| unrestricted replication of human cytomegalovirus in hydrocortisone-treated macrophages. | monocytes differentiated in the presence of phytohemagglutinin p-stimulated t cells could be infected with human cytomegalovirus ad169 and produced low levels of infectious virus. additional treatment with therapeutic levels of hydrocortisone resulted in a 10- to 100-fold increase in infectious virus production. hydrocortisone-treated cells demonstrated immediate-early protein kinetics similar to that observed with human fibroblasts, whereas a delay of up to 24 h was observed with untreated cell ... | 1991 | 1656103 |
| sodium butyrate-inducible replication of human cytomegalovirus in a human epithelial cell line. | replication of human cytomegalovirus (hcmv) in a human epithelial thyroid papillary carcinoma cell line (tpc-1) was restricted. however, pretreatment of these cells with 5 mm sodium butyrate (nab) for 24 hr before infection enhanced both hcmv yield and infectious center titer to a similar level of that seen in human embryonic lung fibroblast cells. immunofluorescence staining, gel electrophoresis, and northern blot analysis revealed that tpc-1 cells are nonpermissive for expression of hcmv major ... | 1991 | 1656587 |
| the glycoproteins of the human herpesviruses. | the herpesvirus family contains several important human pathogens. human herpesviruses include herpes simplex virus type 1 and 2, varicella-zoster virus, human cytomegalovirus, epstein-barr virus and human t-cell lymphotropic virus. the general property of herpesviruses is their ability to establish latency and to be periodically reactivated. all human herpesviruses contain a subset of genes encoding viral glycoproteins that are clearly homologous, and their similarity is significantly greater a ... | 1991 | 1657514 |
| [endomyocardial biopsy in the heart transplant patient: the state of the art]. | data regarding 2176 endomyocardial biopsies (emb) (nov. '85-dec. '89) performed in 164 transplanted hearts (4 etherotopic) from 158 patients (6 retransplants) are herein reported. this study was aimed to evaluate: 1) incidence and characteristics of early ischemic myocardial damage. 2) the influence of different immunosurveillance protocols on incidence, degree and aggressiveness of acute rejection and the inflammatory infiltrate composition. 3) the immunophenotype of infiltrating cells in moder ... | 1991 | 1804749 |
| humoral immune response to human cytomegalovirus proteins: a brief review. | although human cytomegalovirus (hcmv) has a genome of 150 x 10(6) da, and a protein-coding content of over 200 open reading frames, few viral proteins seem able to elicit a strong antibody response in the natural host during viral infection. the immunodominant polypeptides include a component of 72 kda among immediate early proteins, a polypeptide of 52 kda among delayed early proteins and a glycoprotein complex of 58 and 93-130 kda and two phosphoproteins of mol. wt 150 and 65 kda among the str ... | 1991 | 1657515 |
| [investigation of human cytomegalovirus infection in 103 cases with history of abnormal pregnancy]. | human cytomegalovirus (hcmv)-igg, igm antibodies were detected by indirect elisa in 103 serum specimens from women with history of abnormal pregnancy. the results showed that the positive rates of hcmv-igg and igm of abnormal pregnant women were 90.29% and 13.59% respectively. the positive rate of hcmv-igm in abnormal pregnancies was higher than that of normal pregnancies (4.07%, p less than 0.001). this suggested that the probability of abnormal pregnancy in hcmv-igm positive women was higher t ... | 1991 | 1657549 |
| expression of hla class i heavy chains and beta 2-microglobulin does not affect human cytomegalovirus infectivity. | human cytomegalovirus (hcmv) purified from urine or tissue culture supernatant has been reported to contain beta 2-microglobulin (beta 2m), which forms the light chain of hla class i molecules. it has been postulated that hcmv covered with beta 2m binds to hla class i alpha-chains at the cell surface. in the present study we used transfected human and mouse cell lines expressing distinct allelic forms of hla class i and beta 2m to determine whether hla class i molecules could act as cellular rec ... | 1991 | 1658201 |
| induction of an endothelial cell growth factor by human cytomegalovirus infection of fibroblasts. | human cytomegalovirus (hcmv) induction of growth factors in fibroblasts was investigated after establishing serum-free culture conditions conducive to viral replication. hcmv infection induced a type ii heparin-binding growth factor which stimulated human endothelial cell proliferation. | 1991 | 1658202 |
| unusual cell specific expression of a major human cytomegalovirus immediate early gene promoter-lacz hybrid gene in transgenic mouse embryos. | transgenic mice carrying the human cytomegalovirus immediate early gene promoter driving the e. coli lacz gene displayed an unusual cell specific expression of beta-galactosidase during development. lacz expression was first detected in cells lining the apex of the neural fold of day 8.5 embryos. by day 10 of gestation, expression was prominent in the spinal ganglia, the ganglia of cranial nerves v, vii, viii, ix, and x, in a line of cells marking the ventrolateral pathway adjacent to the dermam ... | 1991 | 1659441 |
| the n-terminal 513 amino acids of the envelope glycoprotein gb of human cytomegalovirus stimulates both b- and t-cell immune responses in humans. | host defense against human cytomegalovirus (hcmv) involves both humoral and cell-mediated immunity. in this report, human immune responses to glycoproteins encoded by the hcmv gb homolog gene have been examined by using glycoproteins purified by immunoaffinity from hcmv virions and recombinant proteins expressed by vaccinia viruses containing either the entire gb open reading frame or a c-terminal deletion mutant, gbm165, coding for the n-terminal 513 amino acids of gb. neutralizing antibodies, ... | 1991 | 1847481 |
| [application of polymerase chain reaction in amplification of human cytomegalovirus b gene code region]. | a nucleic acid amplification procedure, the polymerase chain reaction (pcr), has been used to amplify the human cytomegalovirus (hcmv) b gene code region, and its glycoprotein 52 kd antigenic domain. the primers used in the pcr assay were based on a conserved region of the hcmv b gene sequence. by using primer 1 (5'-aaagaattcatggaatccaggatctg-3', upstream nucleotides 157 to 2877), primer 2 (5'-aaagaattcatgaacgtgaaggaatcg-3', upstream nucleotides 1846 to 2877), and primer 4(5'-ataaagcttaatcagacgt ... | 1991 | 1664811 |
| acyclic analogues of pyrazofurin: syntheses and antiviral evaluation. | acyclic analogues of pyrazofurin, including 4-hydroxy-3(5)-[( 2-hydroxy-1-(hydroxymethyl)-ethoxy]methyl)-1h-pyrazole-5 (3)-carboxamide (36) and 4-hydroxy-3(5)-[(2-hydroxyethoxy)methyl]-1h-pyrazole-5(3)-carboxamide (27), that possess the side chains of ganciclovir and acyclovir, respectively, were prepared by heating methyl 4-acetoxy-1-acetyl-3-bromomethyl-1h-pyrazole-5-carboxylate (15) and sodium acetate in the requisite alcohols or, for 36, with the sodium alkoxide in dry tetrahydrofuran. these ... | 1991 | 1665755 |
| the dna sequence of the human cytomegalovirus genome. | in the first part of this article we review what has been learnt from the analysis of the sequence of hcmv. a summary of this information is presented in the form of an updated map of the viral genome. hcmv is representative of a major lineage of herpesviruses distinct from previously sequenced members of this viral family and demonstrates striking differences in genetic content and organization. the virus encodes approximately 200 genes, including nine gene families, a large number of glycoprot ... | 1991 | 1666311 |
| in situ elisa for the evaluation of antiviral compounds effective against human cytomegalovirus. | an in situ elisa was developed as an improved procedure over the plaque reduction assay for antiviral susceptibility testing of hcmv. unlike the plaque reduction assay, the elisa can be completed at 4-5 days post-infection. the effective dose (ed50) of ganciclovir (gcv), acyclovir (acv), phosphonoacetic acid (paa), or phosphonoformic acid (pfa), was determined using hcmv strain ad169. the resistance profiles of two laboratory-derived gcv-resistant mutants of hcmv strain ad169 and seven clinical ... | 1991 | 1667788 |
| the 21bp repeat element of the human cytomegalovirus major immediate early enhancer is a negative regulator of gene expression in undifferentiated cells. | the major immediate early regulatory region of human cytomegalovirus (hcmv) has a complex set of dna sites through which both cellular and viral factors coordinately regulate immediate early gene expression. in undifferentiated human teratocarcinoma (t2) cells we have previously shown that major immediate early gene expression is repressed by a differentiation specific nuclear factor mbf1, which binds to the imperfect dyad symmetry located upstream of the enhancer. however, upon differentiation ... | 1991 | 1851560 |
| development and evaluation of a capture elisa for igm antibody to the human cytomegalovirus major dna binding protein. | a new capture elisa (elab) for determination of the igm antibody response to the human cytomegalovirus major dna binding protein (p52) was developed by using a p52-specific monoclonal antibody. as a reference test, a capture elisa using in parallel viral- and cell-control labeled antigens (ela) was employed. general specificity, which was determined on 180 unselected igm-negative sera from an adult population was 100%; stringent specificity, which was evaluated on 108 potentially interfering ser ... | 1991 | 1667791 |
| immunomodulation of human cytomegalovirus infection on interferon system in patients with systemic lupus erythematosus. | on the basis of the research on the relationship between human cytomegalovirus (hcmv) infection and systemic lupus erythematosus (sle) a study of the effects of hcmv infection on the immunological pathogenesis of sle was undertaken. presented in this paper is a brief account of the study. it was found that sle patients with active hcmv infection had significant higher level of serum interferon-alpha (ifn-alpha) than those without active hcmv infection, but the ifn-alpha induction capacity of per ... | 1991 | 1667808 |
| a discrete cis element in the human immunodeficiency virus long terminal repeat mediates synergistic trans activation by cytomegalovirus immediate-early proteins. | the major immediate-early (ie) promoter of human cytomegalovirus directs the expression of several differentially spliced and polyadenylated mrnas that encode isoformic proteins with apparent molecular masses of 55, 72, and 86 kda. all of these proteins are potent transcriptional regulatory proteins. we are interested in the collateral interactions between human cytomegalovirus and human immunodeficiency virus (hiv) in the context of dual infection of a cell. the roles of the specific ie protein ... | 1991 | 1682509 |
| evidence that hiv-1 gag precursor shares antigenic sites with the major capsid protein of human cytomegalovirus. | a rabbit antiserum prepared against disrupted sucrose-banded hiv-1 virus (strain fre-3) reacted with antigens present in nuclear inclusions, pathognomonic for human cytomegalovirus (hcmv). this cross-reactivity was observed in autopsy specimens from individuals infected with cmv, in the presence or absence of co-infection with hiv-1. a towbin immunoassay showed that the serum reacted specifically with the hcmv major capsid protein (mcp, 153 kda), both in the nuclear fraction of infected cells an ... | 1990 | 1691565 |
| effect of intron a from human cytomegalovirus (towne) immediate-early gene on heterologous expression in mammalian cells. | a 2.4 kb fragment of hcmv (towne strain), containing the 5' end of the major immediate-early gene, has been cloned, sequenced, and used to construct a series of mammalian cell expression plasmids. the effects of regulatory regions present on this fragment were assessed using human glycoproteins as reporter molecules. we compared secreted levels of factor viii, t-pa, and hiv-1 envelope glycoproteins in cells transfected with plasmids in which intron a of the immediate-early gene was present or ab ... | 1991 | 1650459 |
| characterization of the dna polymerase gene of human herpesvirus 6. | the construction of a recombinant bacteriophage lambda library containing overlapping clones covering 155 kbp of the 161-kbp genome of the ugandan u1102 isolate of human herpesvirus 6 (hhv-6) is described. the use of degenerate-primer polymerase chain reaction allowed the isolation of a dna probe for the dna polymerase gene of hhv-6, which was subsequently used to isolate and position the pol gene on the physical map of the viral genome. a 4.4-kbp ecori dna restriction fragment containing the po ... | 1991 | 1651403 |
| gene expression from heterologous promoters in a replication-defective avian retrovirus vector in quail cells. | avian retrovirus vectors, with potential for use in avian transformation, were constructed to evaluate the relative efficiency of promoters placed internal to the viral long terminal repeats (ltr). the vectors are replication-defective reticuloendotheliosis plasmids that contain the neomycin phosphotransferase gene under control of the 5' ltr and an internal promoter that directs expression of the chloramphenicol acetyltransferase gene. the internal promoters were the sv40 early, the mouse metal ... | 1991 | 1652136 |
| identification of a transactivating function mapping to the putative immediate-early locus of human herpesvirus 6. | sequencing studies have indicated that the unique component of the human herpesvirus 6 (hhv-6) genome and the unique long segment of the human cytomegalovirus genome are genetically colinear. of particular interest is the identification of a region of local cpg dinucleotide suppression in the genome of hhv-6, a feature conserved in the genomes of human cytomegalovirus, murine cytomegalovirus, and simian cytomegalovirus, and a characteristic of the major immediate-early loci of these viruses. adj ... | 1991 | 1654446 |
| fine specificity of cellular immune responses in humans to human cytomegalovirus immediate-early 1 protein. | cell-mediated immunity is important in maintaining the virus-host equilibrium in persistent human cytomegalovirus (hcmv) infection. the hcmv 72-kda major immediate early 1 protein (ie1) is a target for cd8+ cytotoxic t cells in humans, as is the equivalent 89-kda protein in mouse. less is known about responses against this protein by cd4+ t cells, which may be important as direct effector cells or helper cells for antibody and cd8+ responses. proliferative-t-cell responses to hcmv ie1 were studi ... | 1991 | 1714519 |
| human monoclonal antibodies to cytomegalovirus recognize viral epitopes on the surface of virus-infected cells. | four human monoclonal antibodies directed against human cytomegalovirus were produced by fusing sp2/hpt heteromyeloma cells with peripheral blood lymphocytes, after stimulation in vitro for 6 days. the human hybridomas have been maintained in culture for one year and secrete, when cultured in serum-free medium, between 3.1 and 8.1 micrograms/ml of antibodies/10(6) cells/24 hours. hcv-1 and hcv-2 are igg kappa, while hcv-3 and hcv-4 are igg3 lambda. the four monoclonal antibodies immunoprecipitat ... | 1991 | 1714785 |
| nucleotide sequence and transcriptional mapping of the major capsid protein gene of pseudorabies virus. | the gene encoding the 142-kda major capsid protein (mcp142) of pseudorabies virus (prv) was isolated and sequenced. nucleotide sequence analysis revealed that the mcp142 gene has a single open reading frame of 3993 nucleotides (nt) encoding 1330 amino acids. the 4400-nt major rna from the mcp142 gene was detected in prv-infected cells. the 5' end of the transcript was located 60 nt upstream of the initiation codon. the 3' end of the transcript was located 18 nt downstream of a putative poly(a) s ... | 1991 | 1718089 |
| expression of a human monoclonal anti-hiv-1 antibody in cho cells. | the cdna coding for the light and heavy chains, respectively, of the human monoclonal antibody 3d6 (igg1, kappa), which binds specifically to human immunodeficiency virus-1 (hiv-1) gp41, was inserted into three different mammalian expression vectors and transfected into chinese hamster ovary (cho) cells. transcription was under the control of rous sarcoma virus long terminal repeat (rsv ltr), human cytomegalovirus major immediate early (cmv ie) promoter, and mouse mammary tumor virus long termin ... | 1991 | 1809191 |
| a murine cell line producing htlv-i pseudotype virions carrying a selectable marker gene. | a murine cell line, eh, expressing the gag and pol proteins of moloney murine leukemia virus (mo-mlv) as well as an mo-mlv recombinant genome with a selectable marker (histidinol dehydrogenase), was transfected with a plasmid coding for the gene of the human t-cell leukemia virus type i (htlv-i) envelope precursor (gp62), placed under the control of the human cytomegalovirus immediate early promoter. one clone, t. 14, was recovered, in which gp62 rna and protein were detected. supernatant from t ... | 1991 | 1845835 |
| human cytomegalovirus ie2 negatively regulates alpha gene expression via a short target sequence near the transcription start site. | repression of human cytomegalovirus alpha (immediate-early) gene expression is under the control of the viral ie2 gene. here we show that ie2 negatively regulates gene expression directed by the strong cytomegalovirus enhancer via a specific 15-bp target sequence (which we term cis repression signal [crs]). this crs is located between -14 and +1 relative to the transcription start site and will function in an orientation-independent fashion, consistent with repression occurring at the transcript ... | 1991 | 1846203 |
| a cis-acting element in the major immediate-early (ie) promoter of human cytomegalovirus is required for negative regulation by ie2. | the major immediate-early promoter (miep) of human cytomegalovirus (cmv) contains a number of different enhancer elements in both repetitive and nonrepetitive sequences that influence the level of downstream transcription. this report describes a cis-acting element in the miep that responds to negative regulation by the ie2 gene product. deletion analysis demonstrated that the cis-acting repressor element is located between the tata box and the transcription initiation site from -13 to -1. the d ... | 1991 | 1846204 |
| comparative inhibitory effects of nucleoside analogues on different clinical isolates of human cytomegalovirus in vitro. | various nucleoside analogues were examined for their inhibitory effects on plaque formation of the laboratory strain ad-169 and 11 clinical isolates of human cytomegalovirus (cmv) in human embryonic fibroblast (mrc-5) cells. the most selective inhibitors of cmv replication were 9-(1,3-dihydroxy-2-propoxymethyl)guanine (dhpg), (s)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine (hpmpc), and (s)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine (hpmpa). the 50% effective concentrations (ec50) for cmv ... | 1991 | 1846389 |
| cytomegalovirus nucleic acid distribution within the human vascular tree. | the presence of human cytomegalovirus (hcmv) nucleic acids was demonstrated in almost all major arteries of hcmv-seropositive patients by polymerase chain reaction. the amount of hcmv nucleic acids present in the arterial wall was calculated to be less than 2 viral genomic equivalents per 2500 human genomic equivalents. no relation was observed between the presence of hcmv nucleic acids in the arterial wall and the existence of atherosclerotic changes. no differences were observed in the number ... | 1991 | 1848040 |
| cellular localization of human cytomegalovirus reactivation in the cervix. | | 1991 | 1849167 |
| the detection of human cytomegalovirus immediate early antigen in peripheral blood leucocytes. | recently, van der bij et al. (1988) reported that active human cytomegalovirus (hcmv) infection could be diagnosed by the detection of hcmv immediate early antigen (iea) directly in the peripheral blood leucocytes of renal transplant recipients. however, the indirect peroxidase technique used resulted in high background staining due to endogenous peroxidase activity and thus the detection of hcmv-iea positive leucocytes, which are sometimes present in extremely low numbers, was not always reliab ... | 1991 | 1849539 |
| three mdbp sites in the immediate-early enhancer-promoter region of human cytomegalovirus. | mdbp, a mammalian sequence-specific dna-binding protein, was found to recognize two sites in the major immediate-early (ie) enhancer of human cytomegalovirus. the recognition sequence for mdbp at each of these sites was localized to 14 bp by studying the effects of limited g methylation, depurination, depyrimidination, or deoxyribose modification on the ability of these sites to bind to mdbp. in addition to the two high-affinity mdbp sites in the enhancer, one low-affinity mdbp site was detected ... | 1991 | 1850934 |
| (+-)-(1 alpha,2 beta,3 alpha)-9-[2,3-bis(hydroxymethyl)-cyclobutyl] guanine [(+-)-bhcg or sq 33,054]: a potent and selective inhibitor of herpesviruses. | (+-)-(1 alpha,2 beta,3 alpha)-9-[2,3-bis(hydroxymethyl)cyclobutyl] guanine [(+-)-bhcg or sq 33,054] is a newly synthesized nucleoside analog with potent and selective antiviral activity against members of the herpesvirus group, including human cytomegalovirus. the activity against a thymidine kinase deficient hsv-2 mutant was 25-fold poorer than against the parent virus, suggesting that phosphorylation is an important prerequisite for antiviral activity against hsv-2. (+-)-bhcg is readily phosph ... | 1990 | 2159261 |
| expression of a human cytomegalovirus receptor correlates with infectibility of cells. | previous studies have demonstrated that human cytomegalovirus (hcmv) specifically binds to a fibroblast membrane glycoprotein(s) with a molecular mass from 30 to 34 kda. in this study, the distribution of the putative receptor proteins was analyzed in a variety of cell types, including cell types representative of those that are infected in vivo. using a sensitive microbinding assay (to score virus attachment) and an indirect detection method (to score hcmv-binding proteins), we found that the 3 ... | 1991 | 1851872 |
| cytomegalovirus activates transcription directed by the long terminal repeat of human immunodeficiency virus type 1. | proteins encoded by a variety of dna viruses activate gene expression from the promoter within the long terminal repeat (ltr) of the human immunodeficiency virus type 1 (hiv-1). the mechanism by which immediate-early (ie) gene products of human cytomegalovirus (cmv) activate expression from the hiv-1 ltr was examined in transient expression assays in cultures of human cells by using plasmids containing the ltr linked to the bacterial chloramphenicol acetyltransferase (cat) gene and a plasmid exp ... | 1990 | 2159554 |
| an fc receptor for human immunoglobulin g is located within the tegument of human cytomegalovirus. | immunogold electron microscopy has demonstrated that human immunoglobulin g (igg) can bind to the tegument of human cytomegalovirus virions by the fc portion of the molecule. this binding was inhibited by preincubation of the fc probes with protein a. treatment of ad169 virions with triton x-100 allowed release of the fc-binding proteins, which were precipitated and characterized by polyacrylamide gel electrophoresis (page). polypeptides of approximately 69 and 33 kda were recovered and shown by ... | 1991 | 1851889 |
| molecular comparisons of the beta 2-microglobulin-binding site in class i major-histocompatibility-complex alpha-chains and proteins of related sequences. | beta 2-microglobulin (beta 2m) binds non-covalently to the alpha 1, alpha 2 and alpha 3 domains of the alpha-chain of class i major-histocompatibility-complex (mhc) molecules. on the basis of the crystal structures of human leucocyte antigens hla-a2.1 and hla-aw68.1, we have used molecular-graphics analyses to define 44 contact points between 19 alpha-chain residues and 18 beta 2m residues. in 88 other alpha-chain sequences from the hla-a, hla-b, hla-c, hla-d, hla-e, hla-f and hla-g locus produc ... | 1991 | 1859364 |
| progress in vaccine development for prevention of human cytomegalovirus infection. | | 1990 | 2161323 |
| immediate-early transcription regulation of human cytomegalovirus. | | 1990 | 2161324 |
| regulation and tissue-specific expression of human cytomegalovirus. | | 1990 | 2161326 |
| efficient gene transfer and expression in primary b lymphocytes. | in this study we have used a panel of vectors expressing the chloramphenicol acetyltransferase (cat) reporter gene under the control of different regulatory elements to optimize gene transfer and expression in primary b lymphocytes. the moloney murine leukemia virus long terminal repeat (momlv ltr) and the sv40 early region promoters, while functional in transfected plasmacytoma cell lines, did not give rise to detectable cat activity following transfection into primary activated mouse or human ... | 1991 | 1865123 |
| identification of human cytomegalovirus isolates by the polymerase chain reaction. | fifty human cytomegalovirus (hcmv) isolates were recovered from different clinical specimens (buffy coat, throat washing and urine) obtained from fifty patients (23 aids patients, 20 heart transplant recipients, 1 bone marrow transplant recipient, 2 newborns with congenital hcmv infection and 4 immunocompetent individuals with acute hcmv infection). the isolates were previously identified by immunological methods and then examined for identification by the polymerase chain reaction. in parallel, ... | 1990 | 1965006 |
| distribution of linear antigenic sites on glycoprotein gp55 of human cytomegalovirus. | human convalescent serum and bacterial fusion proteins constructed from overlapping open reading frames of the nucleotide sequence encoding the human cytomegalovirus gp55 component of the major envelope glycoprotein complex, gp55-116 (gb), were used to localize antigenic regions recognized by human antibodies. all donor serum analyzed contained antibody reactivity for an antigenic site(s) located between amino acids (aa) 589 and 645, a region containing a previously defined linear site recognize ... | 1991 | 1702157 |
| class i mhc-restricted cytotoxic t lymphocyte recognition of cells infected with human cytomegalovirus does not require endogenous viral gene expression. | the human pathogen cmv, is a major cause of morbidity and mortality in immunocompromised hosts. the cd8+ class i-restricted ctl response to cmv assists in preventing progression of cmv infection to life-threatening disease; however, the viral ag recognized by cd8+ ctl are not well characterized. in general, virus-specific ctl recognize endogenously synthesized viral proteins processed and presented associated with class i mhc molecules. although proteins or inactivated virions have been experime ... | 1991 | 1707922 |
| analysis of interstrain variation in cytomegalovirus glycoprotein b sequences encoding neutralization-related epitopes. | nucleotide sequences of a part of the envelope glycoprotein b (gb) gene of human cytomegalovirus (cmv), encoding epitopes recognized by virus-neutralizing monoclonal antibodies, were determined for 12 distinct clinical strains of cmv after amplification of suitable templates using the polymerase chain reaction. sequence analysis of this region (codons 384-717) revealed that the clinical strains and previously sequenced laboratory strains towne and ad169 belong to one of four variant groups, each ... | 1991 | 1709960 |
| mapping of serologically relevant regions of human cytomegalovirus phosphoprotein pp150 using synthetic peptides. | the entire amino acid sequence of human cytomegalo-virus (hcmv) 150k matrix phosphoprotein (pp150), consisting of 1048 amino acid residues, was divided into 95 overlapping 20 amino acid peptides which were synthesized on polyethylene rods. the rods were subjected to elisa with pooled anti-hcmv-positive and anti-hcmv-negative sera. four peptides recognized by the anti-hcmv-positive pool only were synthesized by the solid-phase method and their reactivity in a conventional elisa, using a panel of ... | 1991 | 1710650 |
| a comparison of transcriptional regulatory element activities in transformed and non-transformed rat anterior pituitary cells. | transformed (gh-3) and non-transformed (p3) rat anterior pituitary cells were compared in their ability to direct expression of plasmids containing a variety of eukaryotic transcriptional regulatory elements (tres). these include the herpes simplex virus thymidine kinase (hsv-tk), rous sarcoma virus long terminal repeat (rsv-ltr), simian virus 40 early (sv-40e), human cytomegalovirus immediate-early (cmv-ie) and mouse metallothionein 1 (mmt-1) tres. chloramphenicol acetyl transferase (cat) gene ... | 1991 | 2050277 |
| the effect of human cytomegalovirus on the production and biologic action of interleukin-1. | the effect of mycoplasma-free human cytomegalovirus (hcmv) on the production and biologic activity of interleukin-1 (il-1) from peripheral blood monocytes was examined. the use of biologic thymocyte assays revealed a time-dependent decrease in the il-1 activity of both hcmv-challenged and control monocytes after initiation of culture. a decrease in the amount of il-1 beta secreted as measured by elisa was also detected. the amount of il-1 beta secreted by hcmv-challenged cells was always greater ... | 1990 | 2165111 |
| long-term expression of human adenosine deaminase in mice after transplantation of bone marrow infected with amphotropic retroviral vectors. | three retroviral vectors, containing a human adenosine deaminase (ada) cdna linked to either the simian virus 40 (sv40) early promoter, the human cytomegalovirus (cmv) immediate early promoter, or the moloney murine leukemia virus (momlv) promoter, were tested for their ability to express ada following infection and transplantation of murine bone marrow. virus was produced by using pa317 amphotropic retrovirus packaging cells. the titer of each of the vectors was similar and no helper virus was ... | 1990 | 2081183 |
| human herpesvirus 6 is closely related to human cytomegalovirus. | a sequence of 21,858 base pairs from the genome of human herpesvirus 6 (hhv-6) strain u1102 is presented. the sequence has a mean composition of 41% g + c, and the observed frequency of cpg dinucleotides is close to that predicted from this mononucleotide composition. the sequence contains 17 complete open reading frames (orfs) and part of another at the 5' end of the sequence. the predicted protein products of two of these orfs have no recognizable homologs in the genomes of other sequenced hum ... | 1990 | 2152817 |
| human cytomegalovirus induces expression of cellular topoisomerase ii. | previous work from our laboratory has suggested that topoisomerase ii is required for replication of human cytomegalovirus (hcmv). in assays of confluent human embryonic lung cells infected with hcmv, topoisomerase ii inhibitors exhibited an irreversible inhibition of viral dna replication. however, northern (rna blot) and western (immunoblot) analyses of confluent uninfected human embryonic lung cells detected very low levels of cellular topoisomerase ii rna and protein. quantitation of human t ... | 1990 | 2152837 |
| isolation of a new herpesvirus from human cd4+ t cells. | a new human herpesvirus has been isolated from cd4+ t cells purified from peripheral blood mononuclear cells of a healthy individual (rk), following incubation of the cells under conditions promoting t-cell activation. the virus could not be recovered from nonactivated cells. cultures of lymphocytes infected with the rk virus exhibited a cytopathic effect, and electron microscopic analyses revealed a characteristic herpesvirus structure. rk virus dna did not hybridize with large probes derived f ... | 1990 | 2153965 |
| effectiveness and cost benefit of a proposed live cytomegalovirus vaccine in the prevention of congenital disease. | congenital infection by human cytomegalovirus (cmv) is presently the leading infectious cause of mental retardation and congenital deafness in the united states. live cmv vaccines in healthy adults have been shown to be safe and to induce immune responses similar to those that occur with natural cmv infection. yet, only recently has a live cmv vaccine been tested for its protective ability. to evaluate the cost benefit and effectiveness of the proposed live cmv vaccine, we compared the following ... | 1990 | 2154028 |
| human cytomegalovirus: pathology, diagnosis, treatment. | | 1990 | 2154081 |
| reciprocal enhancement of gene expression and viral replication between human cytomegalovirus and human immunodeficiency virus type 1. | biological interactions between human cytomegalovirus (hcmv) and the human immunodeficiency virus type 1 (hiv-1) were analysed in transfection and infection experiments, carried out in a human osteogenic sarcoma cell line (hos) and in the same cell line chronically infected with hcmv (e155). when hos and e155 cells were transfected with recombinant plasmids containing the hiv long terminal repeat (ltr) linked to the bacterial chloramphenicol acetyltransferase (cat) gene, ltr-directed cat express ... | 1990 | 2154540 |
| acyclic purine phosphonate analogues as antiviral agents. synthesis and structure-activity relationships. | a series of 9-(phosphonoalkyl)purines, which are analogues of 9-[2-(phosphonomethoxy)ethyl]purines (guanine, pmeg, 1; adenine, pmea, 2), were synthesized. the analogues were tested for activity against herpes simplex virus types 1 and 2 (hsv-1 and hsv-2), human cytomegalovirus (hcmv), rauscher murine leukemia virus (r-mulv), and human immunodeficiency virus type 1 (hiv-1). with variations in the length of the alkyl chain, the optimal activity was achieved with two carbons between the purine base ... | 1990 | 2157012 |
| transactivation of a human cytomegalovirus early promoter by gene products from the immediate-early gene ie2 and augmentation by ie1: mutational analysis of the viral proteins. | expression from a human cytomegalovirus early promoter (e1.7) has been shown to be activated in trans by the ie2 gene products (c.-p. chang, c. l. malone, and m. f. stinski, j. virol. 63:281-290, 1989). using wild-type and mutant viral proteins, we have defined the protein regions required for transactivation of the e1.7 promoter in ie2 and for augmentation of transactivation in the ie1 protein. two regions of the ie2 proteins were found to be essential for transactivation. one near the amino te ... | 1990 | 2157038 |
| immunoradiometric assay for cytomegalovirus-specific igg antibodies: assay development and evaluation in blood transfusion practice. | an immunoradiometric assay (radio-immunosorbent test; rist) for the detection of igg antibodies to human herpesvirus 4 [human cytomegalovirus (cmv)] has been developed. the technique utilizes cmv antigen passively adsorbed to a polyvinyl microtitration plate and a radiolabelled murine monoclonal anti-human igg antibody to detect binding of human antibody to the 'solid phase' reagent. the assay was optimized, and its specificity confirmed by testing paired acute and convalescent sera from patient ... | 1990 | 2157732 |
| sequence requirements for proteolytic processing of glycoprotein b of human cytomegalovirus strain towne. | truncated versions of the human cytomegalovirus (cmv) strain towne glycoprotein b (gb) gene were stably expressed in cho cell lines. the calcium-specific ionophore a23187 inhibited proteolytic cleavage of c-terminal-truncated gb expressed by cell line 67.77. these inhibition studies also showed that the 93-kilodalton cleavage product most likely represents the n-terminal cleavage fragment of gb. the ionophore carboxyl cyanide m-chlorophenyl-hydrazone was used to show that proteolytic cleavage of ... | 1990 | 2159553 |
| human cytomegalovirus phosphoproteins and glycoproteins and their coding regions. | | 1990 | 2161320 |
| immune response to human cytomegalovirus infection. | | 1990 | 2161322 |
| reciprocal enhancement of gene expression and viral replication between human cytomegalovirus and human immunodeficiency virus type 1. | | 1990 | 1965159 |
| infection by human cytomegalovirus and rubella virus of cultured human fetal islets of langerhans. | several viruses are implicated in the pathogenesis of pancreatic beta cell destruction and the onset of insulin-dependent (type 1) diabetes mellitus (iddm). however, it is still controversial as to whether viral infection can exert direct cytopathic effects on beta cells. we have studied the effect of infection by human cytomegalovirus (cmv) and rubella virus on human fetal pancreatic islets in tissue culture. although morphological changes were observed in cmv-inoculated islet cells, such infec ... | 1990 | 1966523 |
| identification of a neutralizing epitope on glycoprotein gp58 of human cytomegalovirus. | human cytomegalovirus contains an envelope glycoprotein of 58 kilodaltons (gp58). the protein, which is derived from a glycosylated precursor molecule of 160 kilodaltons via proteolytic cleavage, is capable of inducing neutralizing antibodies. we have mapped the epitopes recognized by the neutralizing monoclonal antibody 7-17 and a second antibody (27-287) which is not neutralizing. overlapping fragments of the carboxy-terminal part of the open reading frame coding for gp58 were expressed in esc ... | 1989 | 2467992 |
| physical mapping of a temperature-sensitive mutation of human cytomegalovirus by marker rescue. | physical mapping of a temperature-sensitive (ts) mutation of human cytomegalovirus (hcmv) strain ad-169 was attempted here using cloned hindiii restriction endonuclease fragments and the mutant virus. the dna-positive mutant tested (hcmv ts 1585) was successfully rescued by viral dna sequences between 0.277 and 0.303 map units. the product of this gene is apparently a structural protein of molecular weight 40,000. marker rescue could thus be used to establish the physical location of essential h ... | 1990 | 1970260 |
| inactivating and mutagenic effects of nitrosoguanide on human cytomegalovirus. | human cytomegalovirus (hcmv) was exposed for 24 hr to 10-30 micrograms/ml nitrosoguanidine (ng) at different times postinfection. ng treatments performed 24 to 48 hr after infection of human iafp-1 fibroblasts with hcmv interfered most significantly with the replication of the virus. no correlation was found, however, between the inactivating effect of ng at this particular stage of the replication cycle and the induction of hcmv temperature-sensitive mutants. all the 17 hcmv mutant strains isol ... | 1990 | 1980399 |
| the human cytomegalovirus 2.7-kilobase rna promoter contains a functional binding site for the adenovirus major late transcription factor. | we have examined the factors which influence the expression of a major 2.7-kilobase (kb) early transcript encoded by the long repeat of the human cytomegalovirus (hcmv) strain ad169 genome. previously, by deletion analysis, we determined that the promoter for this early rna consisted of multiple cis-acting elements (klucher et al., j. virol. 63:5334-5343, 1989). using extracts prepared from hela cells as well as from infected and uninfected foreskin fibroblasts, we also obtained evidence for the ... | 1990 | 2166813 |
| susceptibility of human herpesvirus 6 to antivirals in vitro. | human herpesvirus 6 (hhv-6) is a recently recognized human herpesvirus isolated from lymphoid cells and thought to be the causative agent for exanthem subitum. using dot blot hybridization, the hhv-6 sensitivity pattern to several antivirals was compared with those of herpes simplex virus type 1 and human cytomegalovirus. hhv-6 most closely resembled cytomegalovirus in that it was relatively resistant to the antiviral effects of acyclovir and bromovinyl-deoxyuridine but sensitive to ganciclovir ... | 1990 | 2167336 |
| recombinant feline herpesviruses expressing feline leukemia virus envelope and gag proteins. | we constructed recombinant feline herpesviruses (fhvs) expressing the envelope (env) and gag genes of feline leukemia virus (felv). expression cassettes, utilizing the human cytomegalovirus immediate-early promoter, were inserted within the thymidine kinase gene of fhv. the felv env glycoprotein expressed by recombinant fhv was processed and transported to the cell surface much as in felv infection, with the exception that proteolytic processing to yield the mature gp70 and p15e proteins was les ... | 1990 | 2168977 |
| [evaluation of igm anti human cytomegalovirus antibody]. | an anti-human cytomegalovirus (hcmv) antibody in 330 sera from patients and normal subjects was examined by elisa. the test results were compared with the results of cf test and there was 99.1% agreement. specific anti-hcmv igm antibody was searched for and only 4 sera were positive. this makes it unlikely that screening for igm anti-hcmv will effectively prevent posttransfusion hcmv infections. | 1990 | 2169545 |
| suppression of herpes simplex virus type 1 reactivation from latency by (+-)-9-([(z)-2-(hydroxymethyl)cyclohexyl]methyl) guanine (l-653,180) in vitro. | latent herpes simplex virus type 1 (hsv-1) infection was induced in human embryonic lung cells in vitro by using a combination of viral replication inhibitors and elevated temperature. under reactivating conditions (superinfection by human cytomegalovirus or temperature manipulation), a nonantiviral thymidine kinase inhibitor (l-653,180) was found to suppress or delay reactivation of hsv-1 from latently infected human embryonic lung cells. l-653,180 alone or in combination with interferon was in ... | 1990 | 2171423 |
| human cytomegalovirus. stimulation of [3h] release from [3h]-arachidonic acid prelabelled cells. | exposure of human lung fibroblasts to human cytomegalovirus (hcmv) stimulated a rapid increase in the release of [3h] from cells prelabelled with radiolabelled arachidonic acid ([3h]aa). maximum stimulation of [3h] release was observed at 20 min postinfection and was quantitatively similar to that induced by the tumor promoter 12-o-tetradecanoylphorbol 13-acetate (tpa: 10 nm) or fetal calf serum (5%). the level of [3h] release was dependent on the multiplicity of infection, and appeared to be me ... | 1990 | 2171460 |
| regulation of cytomegalovirus late-gene expression: differential use of three start sites in the transcriptional activation of icp36 gene expression. | we have investigated the transcriptional regulation of the human cytomegalovirus gamma gene encoding the icp36 family (p52, the major late dna-binding protein). the icp36 transcription unit initiates at three distinct sites which are separated by approximately 50 nucleotides and are differentially regulated during infection. at early times (8 h postinfection), only two of these start sites, the most proximal and distal site, were active whereas at late times (36 h postinfection), the middle star ... | 1989 | 2538657 |
| an adenovirus recombinant that expresses the human cytomegalovirus major envelope glycoprotein and induces neutralizing antibodies. | the gene of the human cytomegalovirus (hcmv) major envelope glycoprotein, gb, was cloned from the towne strain and inserted into adenovirus type 5 downstream of the e3 promoter. the recombinant virus, ad-gb, expressed antigenically related proteins of 58, 30, 25, and 23 kda in a549 and mrc-5 cells; the 58-kda protein had the same mobility as the native gb from hcmv-infected mrc-5 cells and virions. all four proteins were detected by a monospecific polyclonal antiserum and by a monoclonal antibod ... | 1990 | 2172396 |
| identification of the lytic origin of dna replication in human cytomegalovirus by a novel approach utilizing ganciclovir-induced chain termination. | infection with human cytomegalovirus in the presence of the antiviral nucleotide analog ganciclovir results in continuing low-level viral dna synthesis and the accumulation of relatively small fragments of double-stranded progency dna. these fragments consistently proved to represent amplification of sequences from only one small section of the viral genome (ecori-v) lying near the center of the unique l segment. further mapping revealed that the viral sequences represented in these fragments oc ... | 1990 | 2173786 |
| cytomegalovirus-induced pneumonitis and myocarditis in newborn mice. a model for perinatal human cytomegalovirus infection. | genetically determined resistance to the lethal effects of infection with murine cytomegalovirus (mcmv) has been reported previously in adult and newborn mice. we examined the pathogenesis of mcmv infection in resistant (cba, h-2k) and susceptible (balb/c, h-2d) mice infected intraperitoneally on the day of birth. balb/c mice developed a severe interstitial pneumonitis and myocarditis 10 days post-infection. their pulmonary and tissues contained high mcmv titres and large numbers of mcmv-antigen ... | 1990 | 2174234 |
| arabinofuranosylpyrrolo[2,3-d]pyrimidines as potential agents for human cytomegalovirus infections. | protection of the 3'- and 5'-hydroxyl groups of the nucleoside antibiotic toyocamycin (1) with 1,3-dichloro-1,1,3,3-tetraisopropyldisiloxane was followed by (trifluoromethyl)sulfonylation of the 2'-hydroxyl group. a displacement of the resulting triflate ester moiety with lithium chloride, lithium bromide, sodium iodide, and lithium azide in hexamethylphosphoramide was followed by a removal of the disilyl moiety with tetra-n-butylammonium fluoride to afford the appropriate (2'-deoxy-2'-substitut ... | 1990 | 2175356 |
| [cell transformation induced by the human cytomegalovirus (hcmv)]. | studies were conducted on the capacity of some human herpes viruses (hsv, hcmv) to induce cell transformation. the results revealed the peculiarities of the transformation process, characteristic of every virus--cell system. | 1990 | 2176533 |
| nucleotide sequence of the most abundantly transcribed early gene of human cytomegalovirus strain ad169. | cytoplasmic poly(a+)rna was isolated from human embryo fibroblast cells during the early phase of an infection with human cytomegalovirus strain ad169. these preparations contained a single abundant transcript of 2.7 kb which was derived from each of the repeat sequences flanking the long unique region of the virus genome. the gene was unspliced and poly(a+)rna derived from it continued to accumulate in the cytoplasm of cells during the later stages of infection. this gene and the surrounding re ... | 1987 | 2436392 |
| human monoclonal antibodies neutralizing human cytomegalovirus. | hybridomas producing human monoclonal antibodies (mabs) against human cytomegalovirus (cmv) were generated by fusion of human spleen cells and mouse myeloma cells. two of the six mabs obtained neutralized viral infectivity even at concentrations lower than 1 microgram/ml. one mab required complement for neutralization but the other did not. both mabs recognized viral proteins of mr 130,000 and 55,000. furthermore, these neutralizing mabs bound to the surface membrane of cmv-infected cells. these ... | 1987 | 2437245 |
| alteration of protein phosphorylation patterns in cell lines morphologically transformed by human cytomegalovirus. | human fibroblastic cell lines morphologically transformed by either live virus or dna fragments of human cytomegalovirus had altered plasma membrane protein composition; quantitative changes, and gains and losses in protein composition in comparison to normal parent cell lines were detected. these transformed cell lines showed altered total cell protein phosphorylation patterns when compared to parent cell lines. a two to four fold increase in in vivo protein phosphorylation at serine and threon ... | 1987 | 2441849 |
| modulation of interleukin 1 beta gene expression by the immediate early genes of human cytomegalovirus. | the immediate early (ie) genes of human cytomegalovirus (hcmv) can be expressed in monocytes/macrophages and are known to regulate other viral genes. the purpose of these studies was to determine if hcmv ie gene products also modulate expression of a monocyte/macrophage-derived gene, interleukin 1 (il-1) beta. steady-state cell-derived il-1 beta mrna was increased in lipopolysaccharide (lps)-stimulated thp-1 cells when transfected with the hcmv ie1 + 2 genes, when compared to cells transfected w ... | 1990 | 2161430 |
| human cytomegalovirus and monocytes: limited infection and negligible immunosuppression in normal mononuclear cells infected in vitro with mycoplasma-free virus strains. | human cytomegalovirus (hcmv) infection has previously been associated with the production of immunosuppression. the mechanism by which any such immunosuppressive effect might be mediated is unclear but previous work has implicated an effect of the virus on monocytes. we have attempted to characterize the immunosuppressive activity produced by in vitro infection of normal monocytes with hcmv strain ad169. we first examined the ability of hcmv ad169 and recent clinical isolates to infect normal pe ... | 1989 | 2543753 |
| murine herpesvirus 68 is genetically related to the gammaherpesviruses epstein-barr virus and herpesvirus saimiri. | short nucleotide sequence analysis of seven restriction fragments of murine herpesvirus 68 (mhv-68) dna has been undertaken and used to determine the overall genome organization and relatedness of this virus to other well characterized representatives of the alpha-, beta- and gammaherpesvirus subgroups. nine genes have been identified which encode amino acid sequences with greater similarity to proteins of the gammaherpesvirus epstein-barr virus (ebv) than to the homologous products of the alpha ... | 1990 | 2161903 |
| carbon-based immunocytochemistry. a new approach to the immunostaining of epoxy-resin-embedded material. | a carbon-based immunocytochemistry (carbic) method, whereby the epoxy resin is completely removed from a section prior to immunocytochemistry (ic), is described. the resulting absence of embedding medium allows for an optimal access of both primary antibodies and marker systems to structures at various levels throughout the depth of the section. a carbon film evaporated onto the surface of the section before extraction of the resin forms a base to which the section adheres and maintains structur ... | 1990 | 2161930 |
| fine specificities of antibodies in sera and cerebrospinal fluid in herpes virus infections of the central nervous system as detected by the antigen variable immunoblot technique. | applying the immunoblot technique a sensitive and specific method was developed for the detection of intrathecally synthesized antibodies against individual specific proteins that are antigens of various infectious agents causing encephalitis. paired serum and cerebrospinal fluid (csf) samples from five patients with herpes virus infections of the central nervous system (cns) (three herpes simplex virus encephalitis, one varicella zoster virus encephalitis, one zoster ganglionitis) were investig ... | 1990 | 2163408 |
| synthesis, antiproliferative, and antiviral activity of certain 4-substituted and 4,5-disubstituted 7-[(1,3-dihydroxy-2-propoxy)methyl]pyrrolo[2,3-d]pyrimidines. | the sodium salts of 4-chloro- and several 4-chloro-5-substituted-7h-pyrrolo[2,3-d]pyrimidines were treated with [1,3-bis(benzyloxy)-2-propoxy]methyl chloride (6) to provide the corresponding 4-chloro- and 4-chloro-5-substituted-7-[[1,3-bis(benzyloxy)-2-propoxy]methyl]pyrrolo [2,3-d]pyrimidines (7-11). debenzylation with boron trichloride at -78 degrees c furnished 4-chloro- and several 4-chloro-5-substituted-7-[(1,3-dihydroxy-2-propoxy)methyl]pyrrolo[2,3- d]pyrimidines (12.16). subsequent aminat ... | 1990 | 2163454 |
| detection of an ie responsive element(s) in the bamhi j fragment of human cytomegalovirus ad169. | the bamhi j fragment of human cytomegalovirus (hcmv) ad169 located at 0.815 to 0.855 map units in the unique short component of the genome was demonstrated to be responsive to the hcmv ie proteins by using a transient chloramphenicol acetyltransferase (cat) gene expression system. the bamhi j fragment was cloned into a cat gene expression plasmid and then cotransfected with a plasmid that expresses the immediate early (ie) genes of hcmv ad169 into the hcmv permissive cell line mrc-5. the results ... | 1990 | 2164722 |
| syngeneic monoclonal anti-idiotype antibodies that bear the internal image of a human cytomegalovirus neutralization epitope. | two glycoproteins have been identified on human cmv that induce neutralizing antibody; an 86,000-da glycoprotein and a 130,000-, 92,000-, and 50,000-da glycoprotein coimmunoprecipitating complex that appears to be the gb homologue of hsv. we have produced syngeneic monoclonal anti-id antibodies (mab2) of the igm isotype to a cmv-neutralizing monoclonal antibody (mab1) that is known to bind to the 86,000-da glycoprotein on the virion envelope. these mab2 bear the internal image of the original vi ... | 1988 | 2448380 |
| effect of human cytomegalovirus on replication of sv40 origin and the expression of t antigen. | human cytomegalovirus (hcmv) induces replication of the cloned sv40 origin of dna replication in both productive and nonproductive infections. hcmv-induced replication of the sv40 dna origin required the presence of t antigen. human embryonic lung (hel) cells were found to be fully permissive for sv40 origin replication only in the presence of hcmv gene expression. in addition, expression of plasmid encoded sv40 t antigen in hel cells was only induced in the presence of hcmv. cotransfection of t ... | 1990 | 2164739 |
| expression of antisense rna fails to inhibit influenza virus replication. | cell lines were constructed which permanently express influenza virus-specific rna. two approaches were followed. c127 cells were transformed with bovine papilloma virus (bpv) vectors and the resulting cell lines were found to inhibit the replication of influenza virus at low multiplicity of infection (moi 0.05). however, examination of cellular rna using single-stranded probes revealed the presence of both (+)sense and antisense rna transcripts (45-70 copies per cell). in this bpv-based system ... | 1989 | 2481910 |