| development and application of the active surveillance of pathogens microarray to monitor bacterial gene flux. | human and animal health is constantly under threat by emerging pathogens that have recently acquired genetic determinants that enhance their survival, transmissibility and virulence. we describe the construction and development of an active surveillance of pathogens (asp) oligonucleotide microarray, designed to 'actively survey' the genome of a given bacterial pathogen for virulence-associated genes. | 2008 | 18844996 |
| trichosporon mucoides fungemia in a liver transplant recipient: case report and review. | four months after receiving an orthotopic liver transplant, a 51-year-old man was admitted for progressive liver failure and severe hepatocellular necrosis thought to be due to tacrolimus. during his hospitalization he experienced bloodstream infections including fungemia due to trichosporon mucoides and prolonged undulating fever despite antifungal and antibacterial treatment. he underwent removal of the allograft and implantation of another liver. fever continued postoperatively until therapy ... | 2009 | 18983414 |
| identification and tracing of enterococcus spp. by rapd-pcr in traditional fermented sausages and meat environment. | four local small-scale factories were studied to determine the sources of enterococci in traditional fermented sausages. | 2009 | 19120623 |
| tigecycline in combination with other antimicrobials: a review of in vitro, animal and case report studies. | tigecycline has been investigated in combination with other antibacterials against a wide range of susceptible and multiresistant gram-positive and gram-negative bacteria. combinations have been analysed in vitro, in animal models and in human case reports. in vitro, tigecycline combined with other antimicrobials produces primarily an indifferent response (neither synergy nor antagonism). nevertheless, synergy occurred when tigecycline was combined with rifampicin against 64-100% of enterococcus ... | 2009 | 19162449 |
| distribution and diversity of the enterococcal surface protein (esp) gene in animal hosts and the pacific coast environment. | this study sought to evaluate the distribution of the enterococcal surface protein (esp) gene in enterococcus faecium in the pacific coast environment as well as the distribution and diversity of the gene in northern california animal hosts. | 2009 | 19187132 |
| dogs are a reservoir of ampicillin-resistant enterococcus faecium lineages associated with human infections. | ampicillin resistance is a marker for hospital-associated enterococcus faecium. feces from 208 dogs were selectively screened for the occurrence of ampicillin-resistant e. faecium (aref). aref was detected in 42 (23%) of 183 dogs screened in a cross-sectional study in the united kingdom and in 19 (76%) of 25 dogs studied longitudinally in denmark. aref carriage was intermittent in all dogs studied longitudinally. multilocus sequence typing of 63 canine aref isolates revealed the presence of 13 d ... | 2009 | 19233953 |
| application of microbial source tracking methods in a gulf of mexico field setting. | microbial water quality and possible human sources of faecal pollution were assessed in a florida estuary that serves shellfishing and recreational activities. | 2009 | 19457032 |
| [liver abcess and biliary fistula as local complications of acute pancreatitis: approach and treatment]. | acute pancreatitis is frequently associated with the development of local complications: collections, necrosis, pseudocysts and abdominal abscesses. although the development of liver abscesses has been linked to bile duct obstruction or abdominal surgery in patients with chronic pancreatitis, there are few descriptions of liver abscesses associated with an episode of acute pancreatitis. we report the case of a 45-year-old man with a first episode of severe acute alcoholic pancreatitis, complicat ... | 2009 | 19473729 |
| human health risk assessment of penicillin/aminopenicillin resistance in enterococci due to penicillin use in food animals. | penicillin and ampicillin drugs are approved for use in food animals in the united states to treat, control, and prevent diseases, and penicillin is approved for use to improve growth rates in pigs and poultry. this article considers the possibility that such uses might increase the incidence of ampicillin-resistant enterococcus faecium (aref) of animal origin in human infections, leading to increased hospitalization and mortality due to reduced response to ampicillin or penicillin. we assess th ... | 2009 | 19490520 |
| high hydrostatic pressure for disinfection of bone grafts and biomaterials: an experimental study. | autoclaving, heat, irradiation or chemical detergents are used to disinfect autografts, allografts and biomaterials for tissue reconstruction. these methods are often associated with deterioration of mechanical, physical, and biological properties of the bone grafts and synthetic implants. high hydrostatic pressure has been proposed as a novel method preserving biomechanical and biological properties of bone, tendon and cartilage. this is the first study to assess the inactivation of clinically ... | 2009 | 19516918 |
| distribution of drug resistance among enterococci and salmonella from poultry and cattle in ethiopia. | enterococci and salmonella were isolated from feces of chicken in intensive poultry farms and cattle which are maintained following traditional practices. their resistance to different antibiotics was also determined. a total of 298 enterococcal isolates consisting of enterococcus faecium (49.6%), enterococcus durans (26.9%), enterococcus hirea (11.9%), and enterococcus faecalis (11.5%) were obtained. among the enterococci, resistance to erythromycin (ery), clindamicin (cli), amoxicillin (amo), ... | 2010 | 19921457 |
| antilisterial activity of a broad-spectrum bacteriocin, enterocin lr/6 from enterococcus faecium lr/6. | enterocin lr/6, a purified bacteriocin, exhibited broad inhibitory spectrum both against related as well as some food-borne pathogens such as listeria monocytogenes, yersinia enterocolitica, aeromonas sp., shigella sp., and bacillus licheniformis. in this investigation, we have focused on l. monocytogenes as the target organism, as it is not only an important pathogen but can also survive over a wide range of environmental conditions such as refrigeration temperature, low ph, and high-salt conce ... | 2010 | 19957209 |
| production, characterization, and antimicrobial activity of a bacteriocin from newly isolated enterococcus faecium ij-31. | this work aimed to isolate and characterize enterococcus spp. from indigenous dairy products in islamabad, pakistan. by classical microbiological techniques, one strain from a butter sample was identified to be enterococcus faecium, and we designated it e. faecium ij-31. the precise identity of this strain was then established by determining the sequence of its 16s and 23s rrna genes. the sequence homology searches revealed matches with a number of previously reported strains, such as e. faecium ... | 2010 | 20051203 |
| bioactive bromophycolides r-u from the fijian red alga callophycus serratus. | four new bromophycolides, r-u (1-4), were isolated from the fijian red alga callophycus serratus and were identified by 1d and 2d nmr and mass spectroscopic analyses. these compounds expand the known structural variety of diterpene-benzoate macrolides and exhibited modest cytotoxicity toward selected human cancer cell lines. bromophycolide s (2) also showed submicromolar activity against the human malaria parasite plasmodium falciparum. | 2010 | 20141173 |
| antibiotic resistance of probiotic strains of lactic acid bacteria isolated from marketed foods and drugs. | to identify the antimicrobial resistance of commercial lactic acid bacteria present in microbial foods and drug additives by analyzing their isolated strains used for fermentation and probiotics. | 2009 | 20163065 |
| detoxification of aflatoxin b1 and patulin by enterococcus faecium strains. | aim of the present study was to investigate the detoxification of aflatoxin b(1) and patulin from aqueous solution by probiotic culture of enterococcus faecium m74 and commercial culture of e. faecium ef031. the effect of the bacterial viability, incubation time and ph of the medium on the binding ability was tested. also, binding stability was determined by washing the bacteria-mycotoxin complexes with phosphate buffer saline. both m74 and ef031 strains have the ability to remove aflatoxin b(1) ... | 2010 | 20356644 |
| species and strain identification of lactic acid bacteria using ftir spectroscopy and artificial neural networks. | lactic acid bacteria (lab) are a heterogeneous group of micro-organisms with high relevance for fermented food and beverages as well as human and animal health. identification of lab is complicated by species richness, a high degree of heterogeneity between species and the presence of very closely related species at the same time. in the present study we developed identification systems based on ftir spectra and artificial neural networks (anns) for species identification of 92 lab species out o ... | 2010 | 20422658 |
| inactivation of methicillin-resistant staphylococcus aureus (mrsa) and vancomycin-resistant enterococcus faecium (vre) on various environmental surfaces by mist application of a stabilized chlorine dioxide and quaternary ammonium compound-based disinfectant. | contaminated environmental surfaces are important reservoirs in the transmission of many human pathogens. although several options exist for disinfecting contaminated environmental surfaces, few are compatible with use on both hard smooth non-porous (hard) and soft porous surfaces (soft) while still offering significant disinfection of the contaminating organisms. this study evaluated the efficacy of mist application of a stabilized chlorine dioxide and quaternary ammonium compound-based disinfe ... | 2010 | 20574884 |
| identification of lactic acid bacteria isolated from the fecal samples of healthy humans and patients with dyspepsia, and determination of their ph, bile, and antibiotic tolerance properties. | the research of novel probiotic strains is important in order to satisfy the increasing request of the market and to obtain functional products in which the probiotic cultures are more active and have better probiotic characteristics than those already on the market. in this study, the probiotic potential of lactobacillus and enterococcus strains isolated from human feces was investigated. one hundred seven strains of lactic acid bacteria were isolated from the fecal samples of 19 adult voluntee ... | 2010 | 20668388 |
| infectious complications in the early postoperative period in liver transplant patients. | liver transplantation (ltx) is a technically well established procedure in acute and in end-stage liver diseases. however, opportunistic infections remain one of the important complications in short and long-term outcome of ltx patients. bloodstream and pulmonary infections are the major cause of death in the first year following liver transplantation. due to extended use of chinolons and third generation cephalosporines there is a shift towards multidrug-resistant bacteria including methicillin ... | 2010 | 21037551 |
| conservation of ebp-type pilus genes among enterococci and demonstration of their role in adherence of enterococcus faecalis to human platelets. | ebp are endocarditis and biofilm associated pili of enterococcus faecalis that are also important in experimental utis. our analyses, using available genomes, found that the ebp locus is unique to enterococci. in e. faecalis, the ebp locus is very highly conserved and only 1/473 e. faecalis isolates tested lacked ebpabc while only 1.2% had the bee pilus locus. no other pilus-encoding operon was identified in 55 available genomes indicating that the vast majority of e. faecalis (unlike enterococc ... | 2011 | 21502588 |
| impact of medicated feed on the development of antimicrobial resistant bacteria in integrated pig-fish farms in vietnam. | integrated livestock-fish aquaculture utilizes animal excreta, urine, and feed leftovers as pond fertilizers to enhance growth of plankton and other microorganisms eaten by the fish. however, antimicrobial resistant bacteria may be transferred and develop in the pond due to selective pressure from antimicrobials present in animal feed, urine, and feces. in an experimental pig-fish farm located in peri-urban hanoi, vietnam, nine piglets were provided feed containing 5 μg/kg pig weight/day of tetr ... | 2011 | 21602395 |
| antibiotic resistance in faecal bacteria isolated from horses receiving virginiamycin for the prevention of pasture-associated laminitis. | enterococcus faecium, a major cause of potentially life-threatening hospital-acquired human infections, can be resistant to several antimicrobials, such that streptogramin quinupristin-dalfopristin (q/d) is one of the few antibiotics still effective. consequently use of the streptogramin virginiamycin as an animal growth promoter was banned in the eu in 1999 as some believed this contributed to the emergence of q/d resistant e. faecium. virginiamycin is advocated for preventing equine pasture-as ... | 2011 | 21676560 |
| screening of lactic acid bacteria and bifidobacteria for potential probiotic use in iberian dry fermented sausages. | the purpose of this study was to select lactic acid bacteria and bifibobacteria strains as potential probiotic cultures during the processing of iberian dry fermented sausages. a total of 1000 strains were isolated from iberian dry fermented sausages (363), and human (337) and pig faeces (300) in different culture media. around 30% of these strains, mainly isolated from iberian dry fermented sausages in lamvab agar, were pre-selected for testing as potential probiotics by their ability to grow a ... | 2008 | 22063588 |