| [the effect of furazolidone or carbadox in the starter mixture for early-weaned piglets]. | for a period of 14 days, piglets from six litters, weaned between the 25th and 28th day of age, were fed the cos 2 starter containing either a premix with furazolidone or carboadox of czechoslovak origin. bentonite hydrosilicate was used as a carrier in both cases. furazolidone administered in the dose of 200 mg per 1 kg of feed prevented diarrhoea, insignificantly increased body weight gain, and decreased the consumption of feed per 1 kg of gain from 5.6 kg in the control to 4.0 kg in the test ... | 1976 | 828986 |
| the effect of antisera on porcine enteropathogenic escherichia coli in ligated segments of pig intestine. | nineteen antisera produced in pigs against 14 enteropathogenic and five nonenterotoxigenic porcine strains of escherichia coli were tested for their ability to inhibit gut loop fluid accumulation induced by homologous and heterologous organisms. in addition, four antisera produced in pigs by an intensive series of intravenous inoculations and three by a less intensive series of intramuscular injections of a polyvalent e. coli vaccine were evaluated. antisera were also produced in rabbits against ... | 1975 | 1089462 |
| [pathogenesis of toxin caused gastroenteritis of vibrios and coli bacteria]. | | 1975 | 1089519 |
| escherichia coli infections in piglets: pathogenesis, virulence and vaccination. | | 1975 | 1091085 |
| [bacteriocinogeny in yersinia enterocolitica]. | bacteriocinogeny was found in 19.8 per cent of y. enterocolitica strains tested. it was designated by the authors as enterocoliticacinogeny and the substance produced by the pathogen was named enterocoliticacine. bacteriocinogeny was found among the collection strains isolated in the south primorye and not among the strains isolated abroad. some cultures of y. enterocolitica isolated abroad and in the south primorye were used as indicator strains for revealing enterocoliticacinogeny. enterocolit ... | 1975 | 1091204 |
| enzymatic arginylation of beta-melanocyte-stimulating hormone and of angiotensin ii. | porcine beta-melanocyte-stimulating hormone and angiotensin ii were examined as acceptors in the reaction catalyzed by arginyl-trna-protein transferase. both inhibited enzymatic transfer of [14c]arginine from trna to bovine albumin. inhibition was competitive with albumin and the k-i values were, respectively, 15 and 0.8 mum. the expected arginylated compounds were isolated and characterized. beta-melanocyte-stimulating hormone and its arginylated product had identical activities in the frog epi ... | 1975 | 1091639 |
| the sequence of amino acid residues around the oxidation-reduction active disulfide in yeast glutathione reductase. | a 14-residue peptide containing the oxidation-reduction active cystine residue from yeast glutathione reductase has been isolated from proteolytic digests of the enzyme in which the free sulfhydryl groups had been reacted with n-ethylmaleimide. the sequence of this disulfide-containing peptide was found to be:(see article). the sequence was highly homologous with the active cystine regions in escherichia coli and pig heart lipoamide dehydrogenase. the sequences of three of the postulated four th ... | 1975 | 1092678 |
| some immunological aspects of liver function. | | 1975 | 1093339 |
| preweaning mortality in pigs. 4 diseases of the gastrointestinal tract in pigs. | the incidence of fatal gastroenteropathies in sucking pigs was studied during a 2-year period in 17 sow herds involving 2,936 litters. the results showed that 2.8 per cent of the liveborn pigs died with gastroenteropathies during the sucking and immediate post weaning period. the incidence was lowest in offspring from second-litter sows (table i) and the progeny from large litters tended to have the highest incidence (table ii). fatal gastrointestinal diseases were observed in a total of 17.6 pe ... | 1975 | 1094407 |
| the in vitro and in vivo effects of carrageenin on humoral and cellular factors of natural resistance. | the inhibitory effect of carrageenin, a sulfated algal polygalactose, on humoral factors of natural resistance is discussed. in dependence on dose and time, the influence of non-specific and specific bacteriolysis, on the in vitro and in vivo opsonic activity and on the course of infection was studied. | 1975 | 1095463 |
| relationships of antibiotics in animal feeds and salmonellosis in animals and man. | | 1975 | 1095537 |
| the effects of feeding tetracycline, nitrovin and quindoxin on the drug-resistance of coli-aerogenes bacteria from calves and pigs. | | 1975 | 1095540 |
| [problems of resistance (author's transl)]. | the results of sensitivity tests performed during the period from 1970 up to 1973 inclusive are compared with those obtained in pervious years. this showed that resistance to penicillin had increased in staphylococci causing bovine mastitis. during each single year, however, there was a decrease. s. typhimurium isolated from cattle showed increased resistance to chloramphenicol, neomycin and ampicillin; s. dublin showed an increase in resistance to chloramphenicol. e. coli isolated from calves w ... | 1975 | 1096359 |
| enteric colibacillosis: evaluation of strains of escherichia coli utilizing the ligated loop technique in gnotobiotic swine. | the enteropathogenicity of 6 strains of escherichia coli was evaluated via the ligated loop technique in 15 gnotobiotic swine 3 to 4 weeks old. strain enteropathogenicity in gnotobiotic swine, as determined on the basis of visual loop distention and light microscopic evaluation of intestinal sections, compared favorably with that reported in conventional swine, except that jejunal loops of gnotobiotic swine may be somewhat less sensitive to certain strains. in addition, postoperative mortality r ... | 1975 | 1096688 |
| heat-stable escherichia coli enterotoxin production in vivo. | hysterectomy-derived, colostrum-deprived piglets were infected with enterotoxigenic escherichia coli on day 4 of life. samples of feces and intestinal contents were collected and tested in infant mice for enterotoxic activity. positive enterotoxic responses were observed in mice given filtrates of feces and intestinal contents from piglets infected withe enterotoxigenic e. coli known to produce heat-stable enterotoxin but not heat-liabile enterotoxin in vitro. it is concluded that heat-stable en ... | 1975 | 1097335 |
| a field study of coliform mastitis in sows. | three sows with agalactia and mastitis were compared with 3 normally lactating sows at 8, 16, 24, and 32 hours postpartum. bacteriologic examination of milk resulted in isolation of klebsiella pneumoniae or escherichia coli from affected glands of all 3 agalactic sows. milk from clinically normal glands of affected sows and from glands of normal sows was culture-negative for coliforms. affected sows had higher rectal temperatures and lower milk yields, and pigs from affected sows had lower mean ... | 1975 | 1097381 |
| in vitro study of the influence of virginiamycin and spiramycin on the composition and biochemical activities of the gastrointestinal flora of piglets. i. influence on the composition of the flora. | in vitro fermentations with the gastro-intestinal flora of 18 treated piglets (control, virginiamycin, spiramycin) were carried out to study the influence of nutritional doses of virginiamycin and spiramycin on the composition of the microflora. each total flora was used for three different incubations: control, 50 ppm virginiamycin, 50 ppm spiramycin. to obtain comparable results with experiments in vivo, an artificial fermentation medium was made on the basis of several chemical analyses of in ... | 1975 | 1098325 |
| factors involved in bactericidal activity of sheep serum. | the bactericidal activity of blood, plasma, and serum from sheep was measured with 3 strains of escherichia coli. plasma showed greater bactericidal activity than blood or serum for strains o78:k80(b) and lilly. the antibody-complement system of normal serum killed the rough strain, lilly, but neither antibody nor complement alone were bactericidal. antibody, complement, and an additional heat-stable cofactor were necessary for killing strain o78:k80(b). strain 3662 was not susceptible to the le ... | 1975 | 1098531 |
| relationships of intestinal enzymes and serum antitoxin to the pig response to escherichia coli enterotoxin. | the intestinal loop technique was used to evaluate the response of three week old piglets to the heat labile (lt) and the heat stable (st) enterotoxins produced by escherichia coli f11(p155). the serum anti-lt activity and the lipase, amylase and trypsin activities in the jejunal lumen of these pigs were determined. piglets responded independently ti each toxin and no relationship between these responses and serum anti-lt activity or the enzyme activities of the jejunal content could be demonstr ... | 1975 | 1100203 |
| [goblet cells in spontaneous colibacteriosis in pigs]. | this work is a part of the studies on the intestinal barrier undertaken to cast some more light on the pathogenesis of colibacteriosis in pigs. the objective of the work was to investigate the goblet cells in the intestines of pigs ill with spontaneous colibacteriosis in the form of oedema disease and gastrointestinal form. the investigations were carried out on 26 pigs segregated into 3 groups: i group -- typical oedema disease (12 pigs), ii group -- colibacteriosis in the gastrointestinal form ... | 1975 | 1101236 |
| leptospirae of serotype lora of the serogroup australis isolated for the first time from swine in the netherlands. | leptospirosis in swine, caused by serotype lora (serogroup australis) was detected both serologically and by culture. the most important symptoms consisted in abortion during the final month of gestation and the birth of dead or not viable piglets. | 1975 | 1101443 |
| [the infectious (plasmidic) bacterial antibiotic resistance]. | | 1975 | 1102479 |
| [course of beta-hemolytic e. coli carrier state in piglets during weaning]. | the investigations were carried out on 22 sows and 44 piglets (one sow and 2 piglets from each litter). the pigs were divided into 4 groups according to the different additions to their diet during weaning which might have an effect on the carrier state and dissemination of beta-haemolytic e. coli. group i was given a feed concentrate and separated milk, group ii - a feed concentrate and sour milk, group iii - a feed concentrate and milk with addition of garlic (200 mg/kg of body weight), group ... | 1975 | 1103097 |
| the role of immune pig colostrum, serum and immunoglobulins igg, igm, and iga, in local intestinal immunity against enterotoxic strain in escherichia coli o55 in germfree piglets. | the protective effect of pig immune colostrum, serum and immunoglobulins igg, igm and iga against the enterotoxic strain of escherichia coli o55, was studied in newborn germfree piglets. this strain produced accumulation of fluid and dilatation of intestine when injected into the ligated ileal segment of germfree piglets, which is considered to be the typical effect of enterotoxins. erosion of the intestinal epithelium and penetration of bacteria into the submucosa were also observed. immune ser ... | 1975 | 1104425 |
| localization of rate-limiting barrier to penetration of endotoxin through nonkeratinized oral mucosa in vitro. | the penetration of tritiated bacterial endotoxin through nonkeratinized oral mucosal epithelium was studied using an in vitro model system and radioautographic tracer techniques. the basement membrane region of the epithelium was the rate-limiting barrier to penetration, and this barrier effect was independent of the direction and duration of penetration as well as any clearance effects of the vasculature. | 1975 | 1104674 |
| [drug resistance and virulence of citrobacter isolated from the focus of piglet intoxication]. | | 1976 | 797596 |
| [chemotherapeutic efficiency of olaquindex in experimental e.coli-diarrhea and enterotoxaemia of piglets]. | | 1976 | 793001 |
| the role of thromboxane a2 in endotoxin-induced aggregation of guinea-pig platelets in vitro. | large concentrations of endotoxin lipopolysaccharides (lps) e. coli o127:b8 and e. coli o26:b6 were needed for induction of platelet aggregation in citrated platelet rich plasma (prp) from normal guinea-pigs. when guinea-pigs were actively immunized with lps e. coli o127:b8 their prp became more sensitive to the aggregatory effects of this type of lps. the increase in sensitivity was rather selective since the responses to adp and lps e. coli o26:b6 remained unchanged. in endotoxin-stimulated pr ... | 1979 | 386740 |
| the influence of plasmid-determined and other characteristics of enteropathogenic escherichia coli on their ability to proliferate in the alimentary tracts of piglets, calves and lambs. | | 1978 | 364065 |
| purification and properties of a pig heart thiolase with broad chain length specificity and comparison of thiolases from pig heart and escherichia coli. | a thiolase (acetyl coa acyltransferase, ec 2.3-1.16) which acts on substrates of various chain lengths (thiolase i) has been purified from pig heart muscle 366-fold to near homogeneity as judged by gel electrophoresis. its molecular weight was estimated to be 200,000 in the absence and 46,000 in the presence of sodium dodecyl sulfate. kinetic measurements with acetoacetyl coenzyme a, 3-ketohexanoyl-coa, 3-ketooctanoyl-coa, and 3-ketodecanoyl-coa yielded apparent km values of 16, 8.3, 2.4, and 1. ... | 1978 | 344310 |
| alternatives to aspirin, derived from biological sources. | | 1977 | 343554 |
| breeding record analysis in pig herds and its veterinary applications--2: experiences with a large commercial unit. | a computer program was used to analyse data from a large commercial pig unit over two years to assess reproductive efficiency and the efficiency of weaner production. monthly inspections of the farm and quarterly post mortem examinations of all dead pigs provided further information. the effects of controlled changes in management on production were assessed. overall the number of piglets reared per sow per year was increased from 15-1 to 17-2 and the eight-week weights improved from a mean of 1 ... | 1977 | 335631 |
| a cyclic adenosine 3',5'-monophosphate-dependent histone kinase from pig brain. purification and some properties of the enzyme. | a cyclic adenosine 3',5'-monophosphate-dependent histone kinase (atp: protein phosphotransferase, ec 2.7.1.37) was isolated from pig brain. the enzyme has been purified 1140-fold; it is homogeneous on polyacrylamide gel electrophoresis and gel filtration. the estimated molecular weight of the enzyme is 120 000. histone kinase dissociates into a catalytic subunit and a regulatory one (molecular weights 40 000 and 90 000, respectively). the catalytic subunit has been obtained in homogeneous state ... | 1975 | 235302 |
| evidence for bactericidal activity of polymorphonuclear leukocytes without phagocytosis. | the relationship between phagocytosis and bactericidal action of polymorphonuclear leukocytes was examined by comparing the functions of cytochalasin d-treated leukocytes with those of the control. measurement of phagocytotic and bacterial dna-degrading activities using escherichia coli prelabeled with [3h]thymidine revealed that phagocytosis and bacterial dna degradation were inhibited by treatment with cytochalasin d to about 50 and 10% of the control, respectively. nevertheless, the bacterici ... | 1979 | 225308 |
| comparison of the effect of various antisera and cobra venom factor on inflammatory reactions in guinea-pig skin. ii. the arthus reaction and the local shwartzman reaction. | the ability of antisera to guinea-pig c3 to inhibit the arthus and local shwartzman reactions was studied. they were found to reduce the non-haemorrhagic component of the active and reversed passive arthus reactions and to delay the appearance of the haemorrhage in the active arthus reaction. cobra venom factor, however, had no effect on the non-haemorrhagic components of these reactions and only delayed the appearance of the haemorrhage of the active arthus reaction. there appeared to be a corr ... | 1975 | 125318 |
| [observations on antibacterial activity of thiamphenicol glycinate acetylcysteinate (author's transl)]. | in vitro thiamphenicol antibacterial activity in the presence of n-acetylcysteine as sodium salt proved to be enhanced when compared with that of the antibacterial alone against both gram-positive and gram-negative bacterial strains. the in vitro results were confirmed in vivo in the guinea-pig, where the sera from animals previously treated by i.m. route with thiamphenicol glycinate acetylcysteinate, showed a higher antibacterial activity than sera from animals treated with thiamphenicol glycin ... | 1979 | 122158 |
| diastereoisomeric glucuronides of oxazepam. isolation and stereoselective enzymic hydrolysis. | oxazepam glucuronide isolated from swine urine by previously published methods was separated into its diastereoisomers by ion-exchange chromatography on a preparative scale. quantitative high-performance liquid chromatography was used to monitor the separation. the two isomers were obtained in analytically pure form and then characterized by elemental analysis, oxazepam content, mass spectrometry, ultraviolet spectroscopy, optical rotation and optical rotatory dispersion-circular dichroism. the ... | 1979 | 35325 |
| [serotype 185 and e. coli o115 - two distinct bioseropathotypes (author's transl)]. | e. coli o115 strain 27w and trabulsi's serotype 185 have been subjected by us to comparing examinations of the serological behaviour of their cell wall and their protoplasmatic antigens, of their biochemical and cultural behaviour and their pathogenicity for the mucous membranes of guinea-pigs. as a result of this inquiry it was found, that e. coli o115 27w and the dysentery-provoking serotype 185 are biological distinct bacteria which only share a minor cross relationship of their o-antigens, a ... | 1975 | 1106060 |
| [protective oral immunization against coli enterotoxemia in swine (author's tranls)]. | the issue of immunity in the case of enteric infections caused by bacteria is still widely unresolved. to clarify this, weaned piglets 6-7 weeks of age were used as a model, because of the particular susceptibility to colienterotoxemia shown by them at this particular age. the experimental design was as follows. the animals were immunized orally and parenterally (i.v. and i.p. routes) by means of a vaccine consisting of bacteria incapable of reproduction. 14 days later, the animals were challeng ... | 1975 | 1106063 |
| [diagnosis of edema disease (colienterotoxemia) in piglets]. | | 1975 | 1108384 |
| the etiology of swine dysentery. iii. the role of selected gram-negative obligate anaerobes. | a disease resembling swinee dysentery occurred in gnotobiotic piglets previously colonized with escherichia coli and given a mixture of five enteric anaerobes and spirochete b-78, orally. no disease occurred in germ-free piglets exposed to four gram-negative obligate anaerobes or to the spirochete; however, if the four respective anaerobes were introduced into gnotobiotic pigs previously colonized with the spirochete, or when the spirochete was introduced into gnotobiotic pigs colonized with the ... | 1975 | 1167199 |
| isolation and metabolism cage system for newborn pigs. | an isolation system was designed and constructed for isolating normal and infected newborn pigs. the system consisted of an outer cage fitted with a biological diffusion filter and a dunk bath entry system and an inner metabolism cage to contain the pig. when tested with s-13 bacteriophage, the isolation and metabolism cage system was at least 99% efficient in preventing the entry or escape of microorganism. a total of 267 escherichia coli-infected newborn pigs have been isolated in these units, ... | 1976 | 1259227 |
| no accounts completely for the oxygenated nitrogen species generated by enzymic l-arginine oxygenation. | we have assessed the stoichiometry of the nitric oxide (no) synthase reaction by using a novel e.p.r. technique. no generated by crude and partially purified no synthase from endothelial cells and escherichia coli-lipopolysaccharide-activated macrophages was trapped by a ferrous diethyldithiocarbamate complex dispersed in yeast. the paramagnetic ferrous mononitrosyl dithiocarbamate complex formed exhibited a characteristic e.p.r. signal at g perpendicular = 2.035 and g parallel = 2.02 with a tri ... | 1992 | 1281408 |
| isolation and characterization of adhesin-defective tnphoa mutants of septicaemic porcine escherichia coli of serotype o115:k-:f165. | non-enterotoxigenic porcine escherichia coli strains belonging to the serogroup o115 have been associated with septicaemia and diarrhoea. putative factors important in the pathogenicity of e. coli of serogroup o115 include fimbrial antigen f165, haemagglutination (mrha), lipopolysaccharide, serum resistance, capsule and production of aerobactin. using tnphoa transposon insertion mutagenesis, two classes of mutants were obtained from e. coli of serotype o115:f165 with respect to the phenotypic ex ... | 1992 | 1282536 |
| nucleotide sequence of cdna for porcine heme oxygenase and its expression in escherichia coli. | the nucleotide sequence of a cdna for porcine heme oxygenase was determined. the open reading frame encoded a polypeptide of 288 amino acid residues with a molecular mass of 33,074 da. a prokaryotic expression plasmid carrying porcine heme oxygenase cdna was constructed and transfected into escherichia coli cells. the full-length heme oxygenase expressed was localized in the bacterial membranes. two small-sized heme oxygenases with no membrane-bound properties were also detected, suggesting that ... | 1992 | 1288499 |
| [expression of recombinant human hemoglobin]. | the well recognized prevalence of infectious agents in products derived from human whole blood and the increasing number of transfusion-transmitted diseases has made urgent the search for a safe alternative to conventional blood transfusion. sources of hemoglobin (hb) different from outdated human bank blood are under active scrutiny in several laboratories. different approaches have been proposed to produce recombinant human hb in bacteria (e. coli), yeast (s. cerevisiae) and transgenic mammals ... | 1992 | 1288539 |
| a ubiquitin conjugating enzyme encoded by african swine fever virus. | the post-translational modification of proteins by covalent attachment of ubiquitin occurs in all eukaryotes by a multi-step process. a family of e2 or ubiquitin conjugating (ubc) enzymes catalyse one step of this process and these have been implicated in several diverse regulatory functions. we report here the sequence of a gene encoded by african swine fever virus (asfv) which has high homology with ubc enzymes. this asfv encoded enzyme has ubc activity when expressed in escherichia coli since ... | 1992 | 1310934 |
| production in escherichia coli of porcine type-i collagenase as a fusion protein with beta-galactosidase. | porcine type-i collagenase (colg-1) was produced as a fusion protein in escherichia coli using the pax5 expression vector. the fusion protein consists of beta-galactosidase at the n terminus joined to a collagen hinge region and a blood-coagulation factor xa cleavage site linked to colg-1. recombinant collagenase (recolg-1) was biologically active in the form of a fusion protein and could be released by treatment with factor xa to yield colg-1 with the authentic n terminus (phenylalanine) found ... | 1992 | 1311701 |
| isolation and sequence analysis of the cdna for pig kidney fructose 1,6-bisphosphatase. | a full-length clone of pig kidney fructose 1,6-bisphosphatase (d-fructose-1,6-bisphosphate 1-phosphohydrolase, ec 3.1.3.11) was isolated by screening a cdna library for complementation of an escherichia coli fbp deletion mutation. the open reading frame of 1011 bases corresponds to 337 amino acids, two more than have been previously reported [marcus, f., edelstein, i., reardon, i. & heinrikson, r. l. (1982) proc. natl. acad. sci. usa 79, 7161-7165]. the extra two amino acids (ala-lys) are locate ... | 1992 | 1313579 |
| mutational analysis of active site residues in pig heart aconitase. | a cdna encoding mature porcine heart aconitase was over-expressed in escherichia coli under the control of a phage t7 promoter. recombinant aconitase purified from e. coli was identical to the enzyme from pig and beef heart in size, [3fe-4s] and [4fe-4s] cluster structure and enzymatic activity. nine amino acid residues in close proximity to the fe-s cluster and bound substrate (lauble, h., kennedy, m.c., beinert, h., and stout, c.d. (1992) biochemistry, in press) were replaced by site-directed ... | 1992 | 1313811 |
| susceptibility to antibiotics of escherichia coli strains isolated from diarrhoeic and non-diarrhoeic livestock in trinidad. | the sensitivity of strains of escherichia coli isolated from calves, piglets, lambs and kids in trinidad to seven antibiotics was determined. two hundred and sixty-four (91.3%) of 289 strains isolated from diarrhoeic animals and 173 (87.4%) of 198 strains from non-diarrhoeic animals exhibited resistance to one or more antibiotics. the difference was not statistically significant (p > or = 0.05; x2). regardless of health status, isolates from lambs were least resistant (75.0%) and those from pigl ... | 1992 | 1339991 |
| [remember escherichia coli mutabile]. | | 1992 | 1340254 |
| restriction fragment length polymorphism and virulence pattern of the veterinary pathogen escherichia coli o139:k82:h1. | escherichia coli o139:k82:h1 strains originating from outbreaks and single cases of oedema disease in pigs were characterized by their genomic restriction fragment length polymorphism (rflp), their virulence pattern, and by the occurrence as well as the genomic distribution of the determinants for hemolysin (hly) and verotoxins (shiga-like toxins; slti, sltii). whereas the rflps revealed considerable variation among the e. coli o139:k82:h1 isolates depending the origin and epidemic source of the ... | 1992 | 1348438 |
| evidence for linkage between the swine l blood group and the loci specifying the receptors mediating adhesion of k88 escherichia coli pilus antigens. | brush borders or enterocytes obtained from the small intestine of 248 pedigreed pigs were tested by adhesion assay in vitro with enterotoxigenic escherichia (e.) coli strains, each expressing one of the three k88 pilus variants k88ab, k88ac and k88ad. all pigs were classified as belonging to one of the four adhesion phenotypes: i--k88ab(-), ac(-), ad(-); ii--k88ab(-), ac(-), ad(+); iii--k88ab(+), ac(+), ad(-); and iv--k88ab(+), ac(+), ad(+). serum or red cells were typed for 15 blood group syste ... | 1992 | 1349202 |
| location of the cytoplasmic epitope for a k(+)-competitive antibody of the (h+,k+)-atpase. | the monoclonal antibody (mab) 95-111 binds the alpha subunit of (h+,k+)-atpase and inhibits the k(+)-atpase activity. to map the epitope, all of the partial sequences of the alpha subunit were expressed in escherichia coli hb101 using rabbit alpha subunit cdna restriction fragments ligated into puex vector. bacterial recombinant lysates were separated by sodium dodecyl sulfate-gel electrophoresis, and the epitope was detected by western blotting. the antibody site was mapped between cys529 and g ... | 1992 | 1382067 |
| cloning and expression of the gal beta 1, 3galnac alpha 2,3-sialyltransferase. | sequence information obtained by nh2-terminal sequence analysis of two molecular weight forms (45 and 48 kda) of the porcine gal beta 1,3galnac alpha 2,3-sialyltransferase was used to clone a full-length cdna of the enzyme. the cdna sequence revealed an open reading frame coding for 343 amino acids and a putative domain structure consisting of a short nh2-terminal cytoplasmic domain, a signal-anchor sequence, and a large cooh-terminal catalytic domain. this domain structure was confirmed by cons ... | 1992 | 1383214 |
| a chromosome 1-specific dna library from the domestic pig (sus scrofa domestica). | chromosomes were prepared from lymphocytes of a male domestic pig and flow-sorted on a dual-laser facs. twenty spots were observed, corresponding to the known pig karyotype of 18 pairs of autosomes plus the x and y. dna was isolated from 10,000 copies of the presumed chromosome 1 spot, restricted with sau3a, ligated into the vector pgem4z, and pcr amplified using universal primers; the products were then re-ligated into puc18. after transformation into escherichia coli, 210,000 independent colon ... | 1992 | 1395721 |
| heterogeneity among heat-labile enterotoxins produced by porcine enterotoxigenic escherichia coli. | the heat-labile enterotoxins (lt) produced by various porcine strains (ltp) of enterotoxigenic escherichia coli were purified to homogeneity and their molecular properties were compared with each other. by double gel diffusion and rabbit skin permeability test, ltps from wt-1 (ltp-wt-1) and bo-149 (ltp-bo-149) strains were antigenically and biologically identical. by polyacrylamide gel electrophoresis with sodium dodecyl sulfate (sds), the mobilities of a and b subunits of ltp (bo-149) were iden ... | 1992 | 1397223 |
| influence of actinobacillus pleuropneumoniae serotype 2 and its cytolysins on porcine neutrophil chemiluminescence. | the effects of actinobacillus pleuropneumoniae serotype 2 and its metabolites on the oxidative activity of porcine neutrophils were studied by using a chemiluminescence technique. viable a. pleuropneumoniae stimulated the production of oxygen radicals by neutrophils. after having reached a peak value, the oxidative activity decreased until a total inhibition of the oxidative activity of the neutrophils was achieved. all effects were neutralized with homologous convalescent-phase pig sera which h ... | 1992 | 1398943 |
| identification of shiga-like toxin type ii producing escherichia coli using the polymerase chain reaction and a digoxigenin labelled dna probe. | epidemiological studies have demonstrated that enterohaemorrhagic strains of escherichia coli which cause the haemolytic uremic syndrome in humans and the oedema disease in pigs more frequently produce shiga-like toxin type ii (slt-ii) than any other member of the shiga-like toxin family. a technique has been developed for the identification of slt-ii producing e. coli using the polymerase chain reaction (pcr) and a digoxigenin (dig)-labelled dna probe to facilitate the early detection and epide ... | 1992 | 1406729 |
| evaluation of three different stb assays and comparison of enterotoxin pattern over a five-year period in swedish porcine escherichia coli. | the pig intestinal loop (pil) assay, inhibition enzyme-linked immunosorbent assay (elisa), and dna hybridization assay were compared for analysis of escherichia coli heat-stable enterotoxin b (stb) on 201 porcine e. coli strains. the dna hybridization had a 95% correlation with the stb elisa and was therefore chosen as the method for subsequent screening of enterotoxin genes: heat labile (lt), heat-stable a (sta), and/or stb. in contrast to the pil assay, both the stb elisa and dna hybridization ... | 1992 | 1424503 |
| observations on production of hemolysin, heat-labile enterotoxin and antimicrobial drug resistance among enterotoxigenic escherichia coli from pigs. | a total of 52 isolates of escherichia coli belonging to enterotoxigenic serotypes from piglets with diarrhea were examined for hemolysis, production of cholera-like enterotoxin (lt) and susceptibility to 10 antimicrobial drugs. a strong association between production of lt and hemolysis was seen. ninety percent of 29 hemolylic isolates were lt+ whereas 100% of 23 nonhemolytic isolates were lt- in a commercial latex agglutination assay. antimicrobial susceptibility tests employing disc diffusion ... | 1992 | 1424558 |
| lipopolysaccharide (lps) inhalation in healthy subjects increases neutrophils, lymphocytes and fibronectin levels in bronchoalveolar lavage fluid. | bacterial endotoxin has been suggested as responsible for the development of subjective symptoms and transient or chronic lung function impairment seen after exposure to organic dusts in cotton mills, poultry houses, swine confinement buildings and saw mills. animal experiments have demonstrated bronchoalveolar neutrophilia being the most prominent cell response in animals following bacterial lipopolysaccharide (lps) inhalation. the present study was conducted to obtain information on some aspec ... | 1992 | 1426208 |
| effect of concentration and source of sulfate on nursery pig performance and health. | the effect of sulfate in drinking water at concentrations of 600, 1,200, and 1,800 mg/l on nursery pig performance and health was evaluated over 28 days on 415 weaned pigs. sodium sulfate and magnesium sulfate were evaluated in combination at concentrations of 600, 1,200, and 1,800 mg/l, and independently at concentrations of 600 and 1,800 mg/l in the drinking water. seven treatment groups and 1 control group were evaluated for mean gain, feed consumption, water consumption, feed conversion, pre ... | 1992 | 1429159 |
| [isolation of vero-cytotoxin-producing escherichia coli from cattle and serotyping and toxin-typing of the isolated strains]. | two hundred and sixty-six piglets with diarrhea (from 4 farms), 73 healthy pregnant pig (from 2 farms), 27 calves with diarrhea (from 9 farms) and 47 healthy milk cows (from 1 farm) were examined for vero-cytotoxin-producing escherichia coli (vtec), and 52, 11, 15 and 67 strains of vtec were isolated from 17 piglets, 11 pregnant pigs, 6 calves and 23 milk cows, respectively. all vtec strains from the piglets produced only vt2vp, while the strains from the healthy pigs did not produce vt2vp, but ... | 1992 | 1431372 |
| molecular characterisation of a dna ligase gene of the extremely thermophilic archaeon desulfurolobus ambivalens shows close phylogenetic relationship to eukaryotic ligases. | a 3382 bp fragment containing a gene for a dna ligase from the extremely thermophilic, acidophilic, and facultatively anaerobic archaeon (archaebacterium) desulfurolobus ambivalens was cloned and sequenced. the deduced amino acid sequence (600 amino acids, 67619 molecular weight) showed 30-34% sequence identity with the atp-dependent eucaryal (eukaryotic) dna ligases of schizosaccharomyces pombe, saccharomyces cerevisiae, the human dna ligase i, and with the vaccinia dna ligase. distant similari ... | 1992 | 1437556 |
| effects of injectable beta-carotene and vitamin a on lymphocyte proliferation and polymorphonuclear neutrophil function in piglets. | newborn piglets were injected with either (1) vehicle; (2) 20 mg beta-carotene; (3) 40 mg beta-carotene; (4) 25,000 iu vitamin a palmitate, or (5) 50,000 iu vitamin a palmitate. blood was collected at 0.5, 1, 3, and 6 weeks of age and lymphocyte proliferation, polymorphonuclear neutrophil (pmn) phagocytosis and intracellular killing of live escherichia coli were measured. lymphocyte proliferation in all piglets was high at birth but decreased thereafter. piglets injected with either high beta-ca ... | 1992 | 1467371 |
| solubilization of growth hormone and other recombinant proteins from escherichia coli inclusion bodies by using a cationic surfactant. | recombinant pig growth hormone (rpgh) was solubilized from inclusion bodies by using the cationic surfactant cetyltrimethylammonium chloride (ctac). the solubilizing action of ctac appeared to be dependent on the presence of a positively charged head group, as a non-charged variant was inactive. relatively low concentrations of ctac were required for rapid solubilization, and protein-bound ctac was easily removed by ion-exchange chromatography. compared with solubilization and recovery of rpgh f ... | 1992 | 1497625 |
| polymerase chain reaction for detection of verocytotoxigenic escherichia coli isolated from animal and food sources. | animals and their by-products have been implicated as important sources of verocytotoxigenic escherichia coli (vtec) associated with disease in humans. vtec comprise a wide range of serotypes and produce a variety of closely related verocytotoxins (vt). a pair of oligonucleotide primers, targeting conserved sequences found in vt1, vt2 and vte genes, was used to develop a polymerase chain reaction (pcr) procedure to detect all types of vtec. supernatants of boiled broth cultures of vtec (223 stra ... | 1992 | 1513344 |
| fate of escherichia coli o157:h7 as affected by ph or sodium chloride and in fermented, dry sausage. | the influence of ph adjusted with lactic acid or hcl or sodium chloride concentration on survival or growth of escherichia coli o157:h7 in trypticase soy broth (tsb) was determined. studies also determined the fate of e. coli o157:h7 during the production and storage of fermented, dry sausage. the organism grew in tsb containing less than or equal to 6.5% nacl or at a ph of 4.5 to 9.0, adjusted with hcl. when tsb was acidified with lactic acid, the organism grew at ph 4.6 but not at ph 4.5. a co ... | 1992 | 1514799 |
| is escherichia coli invading tubuloepithelial cells? | the adhesion of escherichia coli to host epithelium cells is the very first step of urinary tract infections followed by the internalization of the bacteria into these cells. these steps are influenced by several surface antigens or products of the pathogen, e.g. fimbriae or adhesins, k antigen, and hemolysin. the bacterial adherence and the internalization of several isogenic e. coli o18 strains differing in the expression of k5 antigen, hemolysin, and type of fimbriae were measured by using a ... | 1992 | 1520978 |
| cloning and expression of a transferrin-binding protein from actinobacillus pleuropneumoniae. | an expression library was constructed from actinobacillus pleuropneumoniae serotype 7. escherichia coli transformants expressing recombinant proteins were identified by immunoscreening with porcine convalescent serum. one transformant expressing a 60-kda protein (60k protein) in aggregated form was identified. serum raised against the recombinant protein recognized a polypeptide with an indistinguishable electrophoretic mobility in the a. pleuropneumoniae wild type after iron-restricted growth o ... | 1992 | 1541562 |
| expression of porcine complement cytolysis inhibitor mrna in cultured aortic smooth muscle cells. changes during differentiation in vitro. | porcine smooth muscle cells (smc) grown to a high density monolayer culture undergo a morphological transition in which the cells draw away from the substrate and form multicellular nodules. the cells within the nodule resemble smc in the aortic media and in some atherosclerotic plaques. the process of nodule formation is associated with the enhanced production of a secreted 38-kda glycoprotein. to characterize the 38-kda protein and its expression, a cdna clone (pc38k) was isolated by immunolog ... | 1992 | 1544909 |
| expression in vivo and in vitro of the major structural protein (vp73) of african swine fever virus. | the vp73 protein was produced by in vitro transcription and translation from the xho i-bam hi fragment located between the cla i-n and cla i-h fragments of the viral genome. this dna fragment encodes a late mrna of about 2.6 kb detected in infected ms monkey and bhk hamster cells. the transcript was initiated at a site within two bases upstream of the translation initiation codon. the in vitro synthesized polypeptide shows the same molecular weight as the in vivo synthesized polypeptide, suggest ... | 1992 | 1550491 |
| characterization of escherichia coli strains isolated from pigs with edema disease. | escherichia coli strains belonging to serogroups o 138 and o 139 isolated from pigs with edema disease, were characterized with respect to the presence of genes encoding shiga-like toxin i, shiga-like toxin ii and shiga-like toxin iiv (slt i, slt ii and slt iiv). genes coding for the heat-stable and heat-labile enterotoxins (st i and lt i) were also detected. plasmid profiling, restriction enzyme digestion of total dna, and ribotyping were performed for further characterization of the strains. t ... | 1992 | 1557896 |
| 31p nmr spectroscopic studies on purified, native and cloned, expressed forms of nadph-cytochrome p450 reductase. | 31p nmr spectroscopy has been utilized in conjunction with site-directed mutagenesis and phospholipid analysis to determine structural aspects of the prosthetic flavins, fad and fmn, of nadph-cytochrome p450 reductase. comparisons are made among detergent-solubilized and protease (steapsin)-solubilized preparations of porcine liver reductases, showing unequivocally that the 31p nmr signals at approximately 0.0 ppm in the detergent-solubilized, hydrophobic form are attributable to phospholipids. ... | 1992 | 1567869 |
| influence of indomethacin on endotoxin-induced changes in gastrointestinal myoelectrical activity and some haematological and clinical parameters in the conscious piglet. | the effect of indomethacin, administered intravenously at 5 mg/kg, on the changes in gastrointestinal myoelectrical activity, rectal body temperature, clinical appearance and some haematological parameters induced by intravenous bolus injection of endotoxin, at 10 micrograms/kg, was examined in conscious piglets with electrodes implanted in the antrum pylori, duodenum, jejunum and ileum. indomethacin inhibited the endotoxin-induced febrile response and the accompanying clinical signs. however, i ... | 1992 | 1598755 |
| time course of various inflammatory mediators during recurrent endotoxemia. | the time course of thromboxane b2 (txb2), 6-keto-pgf1 alpha (stable metabolite of prostacyclin), tumor necrosis factor-alpha (tnf alpha), platelet activating factor (paf), and interleukin-6 (il-6) formation after three lipopolysaccharide (lps) infusions was studied in pigs over an 18-hr, period. the escherichia coli endotoxin w0111:b4 was injected i.v. into 10 of the test group pigs at a dose of 0.5 micrograms/kg over 30 min at 0, 5 and 10 hr of the experiment. three pigs injected with physiolog ... | 1992 | 1599496 |
| colonization of the small intestine of weaned pigs by enterotoxigenic escherichia coli that lack known colonization factors. | intestinal colonization of 3-week-old weaned pigs by enterotoxigenic escherichia coli (etec) strains that were originally isolated from weaned pigs with fatal diarrhea and that lacked k88, k99, f41, and 987p adhesins (4p- etec) was studied by histologic, immunofluorescent, and electron microscopic techniques. in the first experiment, 16 principal pigs were inoculated orogastrically with etec strain 2134 (serogroup o157: h19) or 2171 (serogroup 0141:h4), and eight control pigs were not inoculated ... | 1992 | 1621335 |
| characterization of the genes encoding beta-ketoadipate: succinyl-coenzyme a transferase in pseudomonas putida. | beta-ketoadipate:succinyl-coenzyme a transferase (beta-ketoadipate:succinyl-coa transferase) (ec 2.8.3.6) carries out the penultimate step in the conversion of benzoate and 4-hydroxybenzoate to tricarboxylic acid cycle intermediates in bacteria utilizing the beta-ketoadipate pathway. this report describes the characterization of a dna fragment from pseudomonas putida that encodes this enzyme. the fragment complemented mutants defective in the synthesis of the coa transferase, and two proteins of ... | 1992 | 1624453 |
| plasmid content and localisation of the stai (stap) gene in enterotoxigenic escherichia coli with a non-radioactive polynucleotide gene probe. | enterotoxigenic escherichia coli (etec) strain p2200 of porcine origin possessed eight possibly plasmid-determined characters (k88+ raf+ hly+ col+ smr tcr su(r) sta+) and six plasmid dna bands of 4.2-93 kb. analysis of the spontaneous loss of characters and the results of matings with other e. coli strains revealed that the k88, raf, hly, smr, tcr and su(r) characters could be transferred, and that the presence of the k88 and raf characters was associated with an 83-kb plasmid. the presence and ... | 1992 | 1629900 |
| [etiological and pathogenetic aspects of diarrhea in piglets following weaning]. | in this paper aetiological and pathogenic aspects of postweaning diarrhoea are discussed. besides earlier data, particular attention is paid to recent results. except specific serotypes of escherichia coli and rotaviruses, feeding regimen as well as components in the feed are important aetiological factors. villus shortening after weaning as well as the level of villus shortening are pathogenetically important. recently recovered villi may be more sensitive to the toxic effects of escherichia co ... | 1991 | 1661032 |
| activation of intestinal brush border guanylate cyclase by aromatic disulphide compounds. | guanylate cyclase in pig intestinal brush border membranes was stimulated by certain aromatic disulphides. the most effective were 6-thioguanine disulphide [(tgs)2], 6-mercaptopurine disulphide, 6,6'-dithiodinicotinic acid, 5,5'-dithiobis-(2-nitrobenzoic acid) and 5-carboxy-2-thiouracil disulphide. (tgs)2 stimulated activity 15-fold when present at 0.1 mm. the optimum concentration for each disulphide was different, and higher concentrations were inhibitory. there was no activation by alkyl disu ... | 1991 | 1673335 |
| human vntr sequences in porcine htf-islands. | mapping of complex genomes has been influenced substantially by the isolation of locus-specific, but repetitive dna elements known as vntrs. since a high gc-content is characteristic of most of these elements one might expect them to be clustered at least partially in cpg-islands. to address this question we have constructed a porcine liver dna noti-linkage library in puc18 using isolated htf-islands. hpaii tiny fragments ranging from less than 100 bp to 1 kb in length were randomly selected and ... | 1991 | 1678358 |
| pericytes of the brain microvasculature express gamma-glutamyl transpeptidase. | the expression of gamma-glutamyl transpeptidase (ggt) is a specific property of the brain capillary endothelium that constitutes the blood-brain barrier. we report here the detection of ggt, not only in endothelial cells, but also in pericytes, demonstrating that a brain capillary-specific pericyte population exists. we raised antibodies to ggt using a porcine brain microvessel ggt-protein-a (staphylococcal protein a) fusion protein as antigen which was expressed in escherichia coli. the immunoh ... | 1991 | 1684747 |
| production of type 1 fimbriae by escherichia coli hb101. | escherichia coli hb101 is frequently used as a host in the cloning of bacterial virulence genes because of its reported lack of virulence determinants such as fimbriae, adhesins and haemagglutinins. however, passage of hb101 in standing broth culture rapidly induced the production of fimbriae which mediated adhesion to hep-2 cells and mannose-sensitive haemagglutination of human and guinea-pig erythrocytes. fimbrial serology, morphology and pilin molecular mass of 18 kda were consistent with tho ... | 1991 | 1686629 |
| [antibody response in mice, rabbits and pigs in response to vaccination with an inactivated oil vaccine containing rotavirus and escherichia coli strains with k88, k99 and 987p fimbrial antigens]. | in trials with mice, rabbits and weanling piglets, four experimental charges of a combined inactivated oil vaccine against diarrhoeas in mammals were tested: the vaccine was to be implanted to sows and it contained porcine rotavirus (prv); two charges also contained bovine rotavirus and bacterins of enterotoxicogenic strains of e. coli with protective antigens k88, k99 and 987p. at low starting antibody titres the twofold i.m. implantation of 0.2 ml vaccine stimulated in mice the production of a ... | 1991 | 1686823 |
| identification and mapping of an immunogenic region of mycoplasma hyopneumoniae p65 surface lipoprotein expressed in escherichia coli from a cloned genomic fragment. | a previously characterized lipid-modified amphiphilic surface protein of mycoplasma hyopneumoniae, p65, has been defined by its reaction with a surface-binding monoclonal antibody (mab) and by its exclusive partitioning into the detergent phase during triton x-114 phase fractionation (k. s. wise and m. f. kim, j. bacteriol. 169:5546-5555, 1987). in the current study, polyclonal mouse antibody (pab) to gel-purified p65 was used to identify recombinant phage plaques expressing p65-related epitopes ... | 1990 | 1695206 |
| the trat lipoprotein as a vehicle for the transport of foreign antigenic determinants to the cell surface of escherichia coli k12: structure-function relationships in the trat protein. | the trat protein is a surface-exposed lipoprotein, specified by plasmids of the incf group, that mediates serum resistance and surface exclusion. the structure and function of the trat protein determined by plasmid r6-5 was probed by genetic insertion of a foreign antigenic determinant, the c3 epitope of polio virus, at residues 61, 125, 180, 200 or 216 of the protein. the chimaeric proteins were transported to the outer membrane and, in three cases, immunoassays with an anti-c3 monoclonal antib ... | 1990 | 1704095 |
| nucleotide sequence of the promoter and fadb gene of the fadba operon and primary structure of the multifunctional fatty acid oxidation protein from escherichia coli. | the primary structure of a multifunctional protein, the large alpha-subunit of the escherichia coli fatty acid oxidation complex, was determined by sequencing the fadb region of the fadba operon. the amino-terminal sequence of this protein had been established by edman degradation. the transcription start site of the fadba operon was located 42 nucleotides upstream of the initiator codon of the fadb gene by primer extension analysis. sequences of -10 and -35 regions of the promoter responsible f ... | 1991 | 1712230 |
| aromatic-dependent salmonella as live vaccine presenters of foreign epitopes as inserts in flagellin. | synthetic oligonucleotides specifying amino acid sequences identified as epitopes of various foreign antigens (cholera toxin subunit b, hepatitis b surface protein and others) have been inserted at an ecorv-ecorv deletion site in a cloned salmonella flagellin gene; the resulting plasmids, when placed in flagellin-negative escherichia coli or salmonella sp. strains, caused production of flagellin expressing the epitope. if the chimeric flagellin allowed formation of flagella, the epitope was expo ... | 1990 | 1714093 |
| localization on the mitochondrial f1 atpase alpha subunit of an epitope masked in the membrane-bound enzyme using a monoclonal antibody and synthetic peptides. | the epitope of the monoclonal antibody 20d6 was localized by n-terminal sequencing of the smallest immunoreactive peptides obtained after cnbr and trypsin cleavage of the f1 alpha subunit of the mitochondrial atpase/atp synthase. immunochemical analysis of overlapping synthetic octapeptides, covering the immunoreactive peptide sequence, has defined the seven-amino-acid sequence recognized by 20d6 as 84egdivkr90. the binding of 20d6 was lost after substituting either i87 by k or s, or r90 by c or ... | 1991 | 1714390 |
| molecular cloning and expression of the cdna coding for a new member of the s100 protein family from porcine cardiac muscle. | we isolated a new calcium-binding protein from porcine cardiac muscle by calcium-dependent hydrophobic and dye-affinity chromatography. it showed an apparent molecular weight of 11,000 on sds-page. amino acid sequence determination revealed that the protein contained two calcium-binding domains of the ef-hand motif. the cdna gene coding for this protein was cloned from the porcine lung cdna library. sequence analysis of the cloned cdna showed that the protein was composed of 99 amino acid residu ... | 1991 | 1722468 |
| use of antibodies against the p36 protein of mycoplasma hyopneumoniae for the identification of m. hyopneumoniae strains. | mycoplasma hyopneumoniae, the principal aetiological agent of porcine enzootic pneumonia, synthesizes a 36 kda protein (p36) which is an early and strong immunogenic factor in experimentally and naturally infected swine. polyclonal antibodies were made against the recombinant p36 protein in rabbits and used for the identification of m. hyopneumoniae by the immunoblot technique. the proteins from the m. hyopneumoniae reference strains and from 13 m. hyopneumoniae field strains isolated from natur ... | 1991 | 1723490 |
| swine and cattle enterotoxigenic escherichia coli-mediated diarrhea. development of therapies based on inhibition of bacteria-host interactions. | enterotoxigenic escherichia coli (etec) frequently occurs in diarrheal disease afflicting domestic animals. in this paper is summarized the research carried out over the last decade on the two important determinants of virulence that plays a role in the development of the infection, namely the colonizing ability of the small intestine mediated by specific fimbrial adhesins acting as lectins and the production of enterotoxins. recent progress in knowledge of the phenomenon led to alternative stra ... | 1991 | 1723693 |
| an enzymatic mutant of shiga-like toxin ii variant is a vaccine candidate for edema disease of swine. | edema disease (ed) of weanling pigs is caused by an infection with escherichia coli that produces shiga-like toxin ii variant (slt-iiv). pathology identical to that caused by ed can be duplicated in pigs that are injected with less than 10 ng of purified slt-iiv per kg of body weight. therefore, slt-iiv was mutated to create an immunoreactive form of the toxin that was significantly reduced in enzymatic activity. initially, purified slt-iiv was treated with formaldehyde which abrogated cytotoxic ... | 1992 | 1730480 |
| stimulation of dna polymerase alpha activity by microtubule-associated proteins. | microtubule-associated protein 2 (map2) isolated from porcine brains stimulated the activity of dna polymerase alpha immunopurified from calf thymus or human lymphoma cells, in a dose-dependent manner. this stimulation was pronounced when activated dna or poly(da).(dt)10 was used as the template-primer. dna polymerase alpha bound to a map2-immobilized column, whereas preincubation of the enzyme with unbound map2 prevented binding to the column. these events suggested that a physical binding occu ... | 1991 | 1742279 |
| systemic and mesenteric o2 metabolism in endotoxic pigs: effect of graded hemorrhage. | normally, supply-dependency of oxygen uptake (vo2) is not demonstrable unless oxygen delivery (do2) is less than a critical value (do2crit) below which vo2 is linearly dependent upon do2. because recent evidence suggests that vo2 is pathologically supply-dependent in endotoxic or septic animals and humans, we sought to determine whether 1) pathological systemic and/or mesenteric oxygen extraction (o2ext) defects occur in a porcine model of endotoxicosis and 2) arterial lactate and ileal intramuc ... | 1991 | 1742860 |
| 1-aminocyclopropane-1-carboxylate synthase in tomato is encoded by a multigene family whose transcription is induced during fruit and floral senescence. | the key regulatory enzyme in the biosynthetic pathway of the plant hormone ethylene is 1-aminocyclopropane-1-carboxylic acid (acc) synthase (ec 4.1.1.14). it catalyzes the conversion of s-adenosylmethionine to acc, the precursor of ethylene. we isolated complementary dna sequences, ptacc2 and ptacc4, for two distinct and differentially regulated acc synthase mrnas expressed in ripe tomato fruit. the authenticity of the clones has been confirmed by expression experiments in e. coli. the predicted ... | 1991 | 1762159 |
| prevalence of antibiotic-resistant escherichia coli in danish pigs and cattle. | the present paper reports on the antibiotic resistance of e. coli isolated from danish piglets and calves in 1987-1988, and compares the results with similar investigations performed during the periods 1971-1972 and 1977-1978. rectal swabs from 52 piglets and from 78 calves were examined. all the animals studied harboured resistant e. coli. this is a significant increase compared to the previously conducted investigations. the number of strains having three or more resistance markers did not dif ... | 1991 | 1772647 |