| prevalence of campylobacter, salmonella, and escherichia coli on the external packaging of raw meat. | during september and october 2002, 3,662 prepackaged raw meat samples were collected to evaluate the extent and nature of microbiological contamination on external surfaces of the packaging, which could potentially cross-contaminate ready-to-eat foods during and after purchase. salmonella was detected on two (<1%) samples of external packaging (both from raw chicken), and campylobacter was detected on 41 (1.1%) samples of external packaging. the external packaging of game fowl exhibited the high ... | 2005 | 15771168 |
| pcr identification of campylobacter coli and campylobacter jejuni by partial sequencing of virulence genes. | the objective of this study was to utilize a multiplex pcr assay for concurrent detection of campylobacter spp. and c. coli or c. jejuni, using probes derived from genes cadf and ceue and an undefined virulence gene. a total of 97 campylobacter strains, isolated from turkey litter (n=74), chicken livers (n=15) and clinical (n=8) samples, were speciated using the pcr-based assay. pcr amplification of the isolates identified a 400-bp cadf gene, conserved in campylobacter species, an 894-bp ceue ge ... | 2005 | 15797819 |
| identification and molecular epidemiology of campylobacter coli isolates from human gastroenteritis, food, and animal sources by amplified fragment length polymorphism analysis and penner serotyping. | campylobacter coli is an infrequently studied but important food-borne pathogen with a wide natural distribution. we investigated its molecular epidemiology by use of amplified fragment length polymorphism (aflp)-based genotyping and penner serotyping. serotype reference strains and 177 danish isolates of diverse origin identified by routine phenotyping as c. coli were examined. molecular tools identified some 12% of field isolates as campylobacter jejuni, emphasizing the need for improved ident ... | 2005 | 15812025 |
| mama-pcr assay for the detection of point mutations associated with high-level erythromycin resistance in campylobacter jejuni and campylobacter coli strains. | twenty campylobacter jejuni and 16 campylobacter coli strains isolated from humans and food/animals, including 17 isolates resistant to erythromycin, were analyzed. a combined mismatch amplification mutation assay-pcr technique was developed to detect the mutations a 2074 c and a 2075 g in the 23s rrna gene associated with erythromycin resistance. all high-level erythromycin-resistant strains examined by dna sequencing carried the transition mutation a 2075 g, whereas no isolate carried the a 20 ... | 2005 | 15927294 |
| prevalence of potential virulence markers in polish campylobacter jejuni and campylobacter coli isolates obtained from hospitalized children and from chicken carcasses. | the pathogenicity of thermotolerant campylobacter species, common food-borne pathogens, depends on certain factors unevenly distributed among strains of different origin. the prevalence of such markers has never been examined in a population of polish campylobacter strains of human and poultry origin. therefore, we analysed the presence of the cadf, cdta, cdtb and cdtc genes and the iam sequence in campylobacter jejuni (n = 115) and campylobacter coli (n = 57) isolates from children with diarrho ... | 2005 | 15947425 |
| inhibition of clostridium perfringens by a novel strain of bacillus subtilis isolated from the gastrointestinal tracts of healthy chickens. | the objectives of this study were to isolate beneficial strains of microorganisms from the gastrointestinal tracts of healthy chickens and to screen them against clostridium perfringens, a causative agent of necrotic enteritis in poultry. one of the bacteria isolated, a strain of bacillus subtilis, was found to possess an anticlostridial factor that could inhibit the c. perfringens atcc 13124 used in this study. the anticlostridial factor produced by b. subtilis pb6 was found to be fully or part ... | 2005 | 16085801 |
| factors affecting the pressure resistance of some campylobacter species. | to compare pressure resistance between strains of campylobacter jejuni, campylobacter coli, campylobacter lari and campylobacter fetus, and to investigate the effect of suspending medium on pressure resistance of sensitive and more resistant strains. | 2005 | 16162138 |
| genomic diversity of campylobacter coli and campylobacter jejuni isolates recovered from free-range broiler farms and comparison with isolates of various origins. | in many industrialized countries, the incidence of campylobacteriosis exceeds that of salmonellosis. campylobacter bacteria are transmitted to humans mainly in food, especially poultry meat products. total prevention of campylobacter colonization in broiler flocks is the best way to reduce (or eliminate) the contamination of poultry products. the aim of this study was to establish the sources and routes of contamination of broilers at the farm level. molecular typing methods (dna macrorestrictio ... | 2005 | 16204541 |
| microbial health risk posed by table eggs in trinidad. | a survey of the microbial quality of table eggs sold in trinidad was conducted. for 23 poultry layer farms each visited twice approximately 1 month apart, 25 pooled eggs constituted a composite sample, for 14 shopping malls each visited twice approximately 1 month apart, six pooled eggs made a composite sample and for a total of 102 other retailers across the country each visited once over a 4-month period, six pooled eggs constituted a composite sample. swabs of egg shells and egg content were ... | 2005 | 16274501 |
| campylobacter in food animals and humans in northern thailand. | cross-sectional, longitudinal, and case-control studies were conducted to describe the epidemiology of campylobacter in chickens, swine, dairy cows, farm workers, nonfarm residents, and children with diarrhea. samples were collected in chiang mai and lamphung provinces of northern thailand from 2000 through 2003. a total of 2,360 samples were processed. results from the cross-sectional study indicated that the prevalences of campylobacter in chickens at the farm, slaughterhouse, and market were ... | 2005 | 16355821 |
| identification of host-associated alleles by multilocus sequence typing of campylobacter coli strains from food animals. | campylobacter coli is a food-borne pathogen associated increasingly with human gastroenteritis. c. coli has a high prevalence in swine, but is isolated also from cattle and poultry. multilocus sequence typing (mlst) systems have been developed to differentiate c. coli strains. although substantial allelic diversity was identified across all seven c. coli mlst loci, no correlations were made in two previous studies between allele or sequence type (st) and the source of the organism. however, this ... | 2006 | 16385134 |
| stable concentrated emulsions of the 1-monoglyceride of capric acid (monocaprin) with microbicidal activities against the food-borne bacteria campylobacter jejuni, salmonella spp., and escherichia coli. | of 11 fatty acids and monoglycerides tested against campylobacter jejuni, the 1-monoglyceride of capric acid (monocaprin) was the most active in killing the bacterium. various monocaprin-in-water emulsions were prepared which were stable after storage at room temperature for many months and which retained their microbicidal activity. a procedure was developed to manufacture up to 500 ml of 200 mm preconcentrated emulsions of monocaprin in tap water. the concentrates were clear and remained stabl ... | 2006 | 16391087 |
| genotyping of campylobacter spp. from retail meats by pulsed-field gel electrophoresis and ribotyping. | to determine the genetic relatedness of campylobacter spp. from retail meat products, and compare the discriminatory power of pulsed-field gel electrophoresis (pfge) and automatic ribotyping. | 2006 | 16405698 |
| antibiotic resistance of campylobacter jejuni and campylobacter coli isolated from hog, beef, and chicken carcass samples from provincially inspected abattoirs in ontario. | campylobacterjejuni is one of the most common causes of bacterial foodborne infection in the united states, and there are reports of resistance of campylobacter spp. to antimicrobial agents used for the treatment of gastroenteritis. the purpose of this study was to determine the antimicrobial resistance patterns of campylobacter spp. isolated from hog, beef, and chicken carcasses from provincially inspected abattoirs in ontario. the agar dilution method was performed to measure antimicrobial res ... | 2006 | 16416896 |
| enumeration and identity of campylobacter spp. in italian broilers. | transmission of campylobacter to humans has been prominently associated with mishandling or improperly preparing contaminated poultry carcasses. the number of organisms per carcass represents an important measure of human exposure to the agent. therefore, we wished to estimate this public exposure over 1 yr among italian broiler carcasses. we sampled 213 broiler carcasses from rinse water samples collected from a single slaughterhouse. groups of carcasses had mean processed weights ranging from ... | 2006 | 16553289 |
| two distinct mutations in gyra lead to ciprofloxacin and nalidixic acid resistance in campylobacter coli and campylobacter jejuni isolated from chickens and beef cattle. | the aim of this study was to identify point mutations in the gyra quinolone resistance determining region (qrdr) of campylobacter coli (n = 27) and campylobacter jejuni (n = 26) that confer nalidixic acid (nal) resistance without conferring resistance to ciprofloxacin (cip). | 2006 | 16553723 |
| quantification of campylobacter on the surface and in the muscle of chicken legs at retail. | the objective of this study was to determine the prevalence and numbers of campylobacter on the skin and in the muscle of chicken legs at retail to examine the external and internal contamination for an exposure assessment. furthermore, the study assessed seasonal influence on campylobacter contamination in chicken legs. of the 140 examined skin samples, 66% were positive, and the internal contamination of 115 sampled chicken legs was 27%. the enumeration of campylobacter on the surface of posit ... | 2006 | 16629016 |
| amplified fragment length polymorphism, serotyping, and quinolone resistance of campylobacter jejuni and campylobacter coli strains from chicken-related samples and humans in taiwan. | the high-resolution genotyping method of amplified fragment length polymorphism (aflp) analysis was used to study the genetic relationships between campylobacter jejuni isolates from chicken-related samples (n = 32) and humans (n = 27) as well as between campylobacter coli isolates from chicken-related samples (n = 27) and humans (n = 5). these isolates were collected between 1994 and 2003 in taiwan. all c. jejuni and c. coli isolates showed highly heterogeneous fingerprints. c. jejuni isolates ... | 2006 | 16629019 |
| prevalence of thermophilic campylobacter infections in humans, chickens and crows in morogoro, tanzania. | prevalence of thermophilic campylobacter infections in humans, chickens and crows was determined in a cross-sectional study that was carried out in urban and rural areas of morogoro region, tanzania during the period of january 2003 to december 2004. a total of 632 human stool samples, 536 cloacal swabs from local and broiler chickens and 22 intestinal contents from crows were screened for presence of thermophilic campylobacters using skirrow's protocol. representative campylobacter jejuni isola ... | 2006 | 16629722 |
| dissemination of fluoroquinolone-resistant campylobacter spp. within an integrated commercial poultry production system. | while characterizing the intestinal bacterial community of broiler chickens, we detected epsilon-proteobacterial dna in the ilea of 3-day-old commercial broiler chicks (j. lu, u. idris, b. harmon, c. hofacre, j. j. maurer, and m. d. lee, appl. environ. microbiol. 69:6816-6824, 2003). the sequences exhibited high levels of similarity to campylobacter jejuni and campylobacter coli sequences, suggesting that chickens can carry campylobacter at a very young age. campylobacter sp. was detected by pcr ... | 2006 | 16672489 |
| genotyping of campylobacter coli and c. jejuni from retail chicken meat and humans with campylobacteriosis in slovenia and bosnia and herzegovina. | thermotolerant campylobacter jejuni and c. coli are one of the major causes of bacterial foodborne enteric infection. consuming and/or handling poultry meat is the most consistent risk factor, linked to the high prevalence of campylobacters in retail poultry meat. the aim of the present study was to ascertain the genetic diversity and/or possible specificity of thermotolerant campylobacter isolates according to species (c. coli, c. jejuni), isolation source (retail chicken meat and human clinica ... | 2006 | 16712997 |
| analysis of campylobacter spp. contamination in broilers from the farm to the final meat cuts by using restriction fragment length polymorphism of the polymerase chain reaction products. | we investigated the genotype diversity and dynamics of campylobacter jejuni and campylobacter coli in six commercial broiler farms during rearing and abattoir processing. in total, 223 c. jejuni and 36 c. coli strains isolated (on farm, transportation crates, carcasses after defeathering, and chicken wing meat at the end of the processing line) were subtyped by pcr-rflp based on flagellin (fla typing) gene. eleven (c. jejuni) and four (c. coli) different rflp patterns were found. multiple c. jej ... | 2006 | 16806554 |
| investigation for possible source(s) of contamination of ready-to-eat meat products with listeria spp. and other pathogens in a meat processing plant in trinidad. | in 2003, there was a recall of three processed (chicken franks, spice ham and turkey ham ready-to-eat (rte) meat products by a large processing plant in trinidad as a result of contamination by listeria monocytogenes. the study was conducted to investigate the possible source(s) of listeria contamination of recalled rte meat products and to determine the prevalence of listeria spp., salmonella spp., escherichia coli and campylobacter spp. in the products and air within the plant. raw and process ... | 2006 | 16943025 |
| bacteriocins reduce campylobacter colonization and alter gut morphology in turkey poults. | campylobacter is a leading cause of food-borne illness in the united states. recent evidence has demonstrated that bacteriocins produced by bacillus circulans and paenibacillus polymyxa reduce cecal campylobacter colonization in broiler chickens infected with campylobacter jejuni. as campylobacter coli is the most prevalent campylobacter isolate recovered in turkeys, the objectives of the present study were to evaluate the efficacy of these bacteriocins against c. coli colonization and their inf ... | 2006 | 16977842 |
| natural presence of campylobacter spp. in various internal organs of commercial broiler breeder hens. | campylobacter are known to cause acute bacterial gastroenteritis in humans. poultry products have been implicated as a significant source of these infections. six experiments were performed to determine whether campylobacter could be isolated naturally from the primary and secondary lymphoid organs, liver/gallbladder, and ceca of commercial broiler breeder hens. broiler breeder hens were acquired from different commercial sources during the early, middle, and late lay cycles. the birds were euth ... | 2006 | 17039849 |
| characterization of macrolide-resistant campylobacter coli isolates from food-producing animals on farms across japan during 2004. | we investigated the susceptibilities against 7 antimicrobial agents in campylobacter jejuni and c. coli isolates from food-producing animals in 2004. in comparison with the results of past surveillance, no significant difference was observed in resistance rates against all of the antimicrobials tested in campylobacter isolates. however, slight increase of erythromycin (em) resistance was found in c. coli isolates from pigs. we examined the mutation of the 23s rrna gene and their susceptibilities ... | 2006 | 17085893 |
| study on the prevalence of campylobacter spp. from chicken meat in hanoi, vietnam. | campylobacter spp. is considered to be the most common bacterial cause of human gastroenteritis worldwide. in developing countries, campylobacter spp. diarrhea is an important cause of childhood morbidity. chicken meat is known to be a major source of campylobacteriosis infection in the world. the purpose of this study was to investigate the prevalence of campylobacter spp. in chicken meat. a total of 100 samples from breast part of chicken carcass were collected from retail market in hanoi. the ... | 2006 | 17135525 |
| a pilot study on post-evisceration contamination of broiler carcasses and ready-to-sell livers and intestines (mala) with campylobacter jejuni and campylobacter coli in a high-throughput south african poultry abattoir. | to assess post-evisceration contamination of broiler carcasses, 300 samples were randomly selected during routine slaughter in the winter of 2004. the samples originated from 50 chicken carcasses, taken directly after evisceration, as well as 25 samples from ready-to-sell packages of fresh intestines (mala) and livers. the samples were taken in batches over a period of 4 weeks to allow randomised sampling from different farms of origin. conventional culture-based detection methods of campylobact ... | 2006 | 17137050 |
| prevalence and antimicrobial resistance of campylobacter jejuni and campylobacter coli isolated from raw chicken meat and human stools in korea. | prevalence of campylobacter in raw chicken meat and human stools and subsequent antibiotic resistance profiles of the pathogenic isolates obtained from 2000 through 2002 were investigated. campylobacter jejuni and campylobacter coli were isolated from 570 of the 923 raw chicken meat samples collected from traditional markets, large retail stores, or department stores in korea, resulting in the isolation rate of 61.8%. a total of 579 campylobacter isolates were obtained from raw chicken (36.3% fo ... | 2006 | 17186659 |
| evaluation of genetic markers and molecular typing methods for prediction of sources of campylobacter jejuni and c. coli infections. | campylobacter jejuni and c. coli isolates from poultry, cattle, and humans were studied using pulsed-field gel electrophoresis (pfge) and pcr of candidate livestock-associated marker genes. human isolates showed 5.7 and 61% overlap with cattle and poultry isolates, respectively, by use of pfge. no unambiguous association was found between marker genes (the cj1321 and cj1324 genes) and livestock-associated isolates. | 2007 | 17220252 |
| survey of chicken abattoir for the presence of campylobacter jejuni and campylobacter coli. | the genus campylobacter is of great importance to public health because it includes several species that may cause diarrhea. these species may be found in water, food and in the intestinal tract of chickens. this study investigated the presence of campylobacter jejuni and campylobacter coli in chicken abattoirs in são paulo state, brazil. a total of 288 samples of feces, feathers, scald water, evisceration water, chiller water, and the rinse water of eviscerated, not eviscerated and chilled carc ... | 2006 | 17221125 |
| efficacy of supplemented buffered peptone water for the isolation of campylobacter jejuni and c. coli from broiler retail products. | broiler retail samples (n=113) were analyzed to determine (i) the effectiveness of buffered peptone water (bpw) supplemented with blood and antibiotics for the isolation of campylobacter jejuni and c. coli, (ii) if a 1:4 enrichment ratio performs similarly as a 1:9 ratio, and (iii) if bpw is similar to bolton broth for enumeration of campylobacter spp. in retail broiler meat using the most probably number (mpn) procedure. chi-square comparison showed that bpw performed similarly as bolton broth ... | 2007 | 17257696 |
| campylobacter coli isolates derived from chickens in senegal: diversity, genetic exchange with campylobacter jejuni and quinolone resistance. | we used the multilocus sequence typing (mlst) method to study the genetic diversity of campylobacter coli isolated from chickens in senegal, and to check the presence of genetic exchange with campylobacter jejuni. in addition, we assessed the resistance of the isolates to ciprofloxacin and nalidixic acid, and their gyra sequences. mlst revealed a low level of diversity and the absence of lineages among c. coli isolates. in addition, an exchange of alleles with c. jejuni was found. twenty percent ... | 2007 | 17258435 |
| novel campylobacter isolation method using hydrophobic grid membrane filter and semisolid medium. | culture procedures for isolation of thermophilic campylobacters from food matrices are complex, labor intensive, and time-consuming. most available methods include the use of antibiotics as selective agents to prevent the growth of competing microflora. a simple procedure for isolation of thermophilic campylobacters after enrichment in rosef's enrichment broth was developed using a hydrophobic grid membrane filter (hgmf) on semisolid medium (ssm). ssm contains no antibiotics, and the hgmf physic ... | 2007 | 17340869 |
| effect of macrolide usage on emergence of erythromycin-resistant campylobacter isolates in chickens. | in this work we conducted both in vitro and in vivo experiments to examine the development and mechanisms of erythromycin (ery) resistance in campylobacter jejuni and campylobacter coli. in vitro plating revealed that both campylobacter species had similar but low spontaneous mutation frequencies (3 x 10(-9) to <5.41 x 10(-10)) for ery resistance. chickens infected with c. jejuni or c. coli were subjected to single or multiple treatments with medicated water containing tylosin (0.53 g/liter), wh ... | 2007 | 17353243 |
| prevalence, numbers, and subtypes of campylobacter jejuni and campylobacter coli in uncooked retail meat samples. | a national quantitative survey of campylobacter jejuni and campylobacter coli in 1,011 uncooked retail meat samples (beef, unweaned veal, chicken, lamb and mutton, and pork) was undertaken from august 2003 to june 2004 to establish baseline proportionality data. the presence, number, and type of campylobacter present in each sample was assessed. prevalences of c. jejuni and c. coli were 89.1% in chicken, 9.1% in pork, 6.9% in lamb and mutton, 3.5% in beef, and 10% in unweaned veal. c. jejuni was ... | 2007 | 17388043 |
| detection of campylobacter jejuni and campylobacter coli in chicken meat samples by real-time nucleic acid sequence-based amplification with molecular beacons. | a nucleic acid sequence-based amplification (nasba) assay based on molecular beacons was used for real-time detection of campylobacter jejuni and campylobacter coli in samples of chicken meat. a set of specific primers and beacon probe were designed to target the 16s rrna of both species. the real-time nasba protocol including the rna isolation was valid for both of the cell suspensions in buffered saline and the artificially contaminated chicken meat samples. the presence of rrna could be corre ... | 2007 | 17395329 |
| detection and genotyping of arcobacter and campylobacter isolates from retail chicken samples by use of dna oligonucleotide arrays. | to explore the use of dna microarrays for pathogen detection in food, we produced dna oligonucleotide arrays to simultaneously determine the presence of arcobacter and the presence of campylobacter in retail chicken samples. probes were selected that target housekeeping and virulence-associated genes in both arcobacter butzleri and thermotolerant campylobacter jejuni and campylobacter coli. these microarrays showed a high level of probe specificity; the signal intensities detected for a. butzler ... | 2007 | 17416693 |
| evaluation of different plate media for direct cultivation of campylobacter species from live broilers. | accurate identification and optimal culturing procedures for campylobacter spp. from live broilers are needed for epidemiological studies. because there is no standardized protocol, we designed and conducted studies to evaluate different selective media for the culturing and isolation of campylobacter spp. from cecal and fecal samples obtained from battery-reared and commercial broilers. five media selective for campylobacter were evaluated: campylobacter agar base, campylobacter, campy-line, mo ... | 2007 | 17575175 |
| campylobacter succession in broiler chickens. | the competitive ability of campylobacter coli or12 over c. jejuni or1 has been examined in experimental broiler chickens following the observation that c. coli replaced an established c. jejuni intestinal colonisation within commercial chicken flocks reared outdoors [el-shibiny, a., connerton, p.l., connerton, i.f., 2005. enumeration and diversity of campylobacters and bacteriophages isolated during the rearing cycles of free-range and organic chickens. appl. environ. microbiol. 71, 1259-1266]. ... | 2007 | 17628357 |
| genotyping of thermotolerant campylobacter from poultry slaughterhouse by amplified fragment length polymorphism. | to examine the occurrence, diversity and transmission of campylobacter in a poultry slaughterhouse. | 2007 | 17650186 |
| commonality and biosynthesis of the o-methyl phosphoramidate capsule modification in campylobacter jejuni. | in this study we investigated the commonality and biosynthesis of the o-methyl phosphoramidate (meopn) group found on the capsular polysaccharide (cps) of campylobacter jejuni. high resolution magic angle spinning nmr spectroscopy was used as a rapid, high throughput means to examine multiple isolates, analyze the cecal contents of colonized chickens, and screen a library of cps mutants for the presence of meopn. sixty eight percent of c. jejuni strains were found to express the meopn with a hig ... | 2007 | 17675288 |
| prevalence of campylobacter jejuni and campylobacter coli in chicken hybrids with different growth rates, reared according to conventional and "free-range" production methods. | | 2007 | 17682919 |
| a collaborative study on a nordic standard protocol for detection and enumeration of thermotolerant campylobacter in food (nmkl 119, 3. ed., 2007). | a nordic standard protocol for detection and enumeration of thermotolerant campylobacter in food has been elaborated (nmkl 119, 3. ed., 2007). performance and precision characteristics of this protocol were evaluated in a collaborative study with participation of 14 laboratories from seven european countries. the laboratories performed qualitative, semi-quantitative and quantitative analyses on samples of chicken meat, cut lettuce, and milk artificially inoculated with different concentrations i ... | 2007 | 17761333 |
| high level of antimicrobial resistance in campylobacter jejuni isolated from broiler chickens in estonia in 2005 and 2006. | the development of antimicrobial resistance in campylobacter jejuni and campylobacter coli is a matter of increasing concern. because campylobacteriosis is transmitted to humans usually via food of animal origin, the presence of antimicrobial-resistant campylobacters in broiler chickens has important public health implications. the aim of our study was to analyze resistance patterns of c. jejuni isolated from fecal samples collected at a large estonian chicken farm, from cecal contents collected ... | 2007 | 17803154 |
| multiplex pcr for direct identification of campylobacter spp. in human and chicken stools. | differentiation between campylobacter jejuni and campylobacter coli is problematic in clinical specimens due to fastidious growth requirements and limited biochemical tests. this study describes a rapid, multiplex pcr protocol for the direct detection and differentiation of c. jejuni and c. coli in stools. an evaluation was carried out of this multiplex protocol based on the detection of cadf (genus specific), and hipo (c. jejuni) and asp (c. coli) genes, using stool from patients with campyloba ... | 2007 | 17893173 |
| quantification and differentiation of campylobacter jejuni and campylobacter coli in raw chicken meats using a real-time pcr method. | campylobacter species are one of the most common causes of bacterial diarrhea in humans worldwide. the consumption of foods contaminated with two campylobacter species, c. jejuni and c. coli, is usually associated with most of the infections in humans. in this study, a rapid, reliable, and sensitive multiplex real-time quantitative pcr was developed for the simultaneous detection, identification, and quantification of c. jejuni and c. coli. in addition, the developed method was applied to the 50 ... | 2007 | 17900077 |
| role of poultry meat in sporadic campylobacter infections in bosnia and herzegovina: laboratory-based study. | to investigate genetic diversity and specificity of campylobacter jejuni and campylobacter coli strains isolated from humans, retail poultry meat, and live farm chickens in zenica-doboj canton, bosnia and herzegovina, and identify the role of poultry meat in sporadic campylobacter infections. | 2007 | 18074419 |
| pulsed-plasma gas-discharge inactivation of microbial pathogens in chilled poultry wash water. | a pulsed-plasma gas-discharge (ppgd) system was developed for the novel decontamination of chilled poultry wash water. treatment of poultry wash water in the plasma generation chamber for up to 24 s at 4 degrees c reduced escherichia coli nctc 9001, campylobacter jejuni atcc 33560, campylobacter coli atcc 33559, listeria monocytogenes nctc 9863, salmonella enterica serovar enteritidis atcc 4931, and s. enterica serovar typhimurium atcc 14028 populations to non-detectable levels (< or = 8 log cfu ... | 2007 | 18095434 |
| antimicrobial resistance of campylobacter spp. isolated from broiler flocks in iceland 2001-2005. | minimum inhibitory concentrations of six antimicrobial agents were determined for one campylobacter sp. isolate from each of the 362 campylobacter-positive commercial chicken flocks in iceland in the years 2001-2005. of all isolates tested, 6.9% were resistant, although none were multiresistant. resistance to ampicillin was most commonly observed (3.6%) followed by resistance to enrofloxacin (3%), nalidixic acid (1.9%), and oxytetracyclin (0.3%), with cross-resistance between enrofloxacin and na ... | 2008 | 18321203 |
| antimicrobial drug resistance as determined by the e-test in campylobacter jejuni, c. coli, and c. lari isolates from the ceca of broiler and layer chickens in grenada. | one hundred and twenty five chickens from grenada, consisting of 77 broilers and 48 layers were examined for carriage of thermophilic campylobacters in their ceca by culture. seventy nine percent of chickens were positive for campylobacters, with an isolation rate of 93.5% for broilers and 56.3% for layers, the difference being significant. sixty-four pure cultures comprising 39 campylobacter coli, 21 campylobacter jejuni, and 4 campyilobacter lari isolates were tested for their resistance again ... | 2009 | 18329712 |
| comparison of antimicrobial resistance of campylobacter jejuni and campylobacter coli isolated from humans and chicken carcasses in poland. | campylobacter-associated gastroenteritis remains an important cause of morbidity worldwide, and some evidence suggests that poultry is an important source of this foodborne infection in humans. this study was conducted to analyze the prevalence and genetic background of resistance of 149 campylobacter jejuni and 54 campylobacter coli strains isolated from broiler chicken carcasses and from stool samples of infected children in poland from 2003 through 2005. nearly all isolates were susceptible t ... | 2008 | 18389707 |
| harmonised monitoring of antimicrobial resistance in salmonella and campylobacter isolates from food animals in the european union. | many member states of the european union (eu) currently monitor antimicrobial resistance in zoonotic agents, including salmonella and campylobacter. according to directive 2003/99/ec, member states shall ensure that the monitoring provides comparable data on the occurrence of antimicrobial resistance. the european commission asked the european food safety authority to prepare detailed specifications for harmonised schemes for monitoring antimicrobial resistance. the objective of these specificat ... | 2008 | 18397331 |
| detection of campylobacter jejuni and campylobacter coli from broiler chicken-related samples using bax pcr and conventional international organization for standardization culture. | in this study, the conventional international organization for standardization (iso) culture method was compared with the dupont qualicon bax system, a high-throughput, rapid molecular assay that can be used to detect several bacterial species, including campylobacter jejuni and campylobacter coli in diverse sample types. standard enrichment culture is a time-consuming process, taking up to 6 days to obtain a confirmed result. rapid molecular assays have been developed that provide results withi ... | 2008 | 18468043 |
| influxed insects as vectors for campylobacter jejuni and campylobacter coli in danish broiler houses. | the vector potential of flies (diptera: brachycera) for spread of campylobacter jejuni and campylobacter coli on 5 danish broiler farms was evaluated in a longitudinal field study from april to november 2004. first, the prevalence of c. jejuni- and c. coli-positive flies was determined in 2,816 flies captured from farm surroundings. each individual fly was macerated, preenriched in bolton broth for 24 h at 42 degrees c, streaked onto modified campylobater blood-free selective agar and incubated ... | 2008 | 18577626 |
| diversity of pulsed-field gel electrophoresis profiles of campylobacter jejuni and campylobacter coli from broiler chickens in france. | in 2003 to 2004, 26 free-range broilers flocks excreting campylobacter were studied for identification of campylobacter species and genotype diversity. seventeen flocks were sampled at the end of the indoor rearing period and 9 before departure to the slaughterhouse after access to an open area. out of 513 isolates, 315 were identified as campylobacter jejuni and 198 as campylobacter coli. pulsed-field gel electrophoresis analysis revealed 35 genotypes for c. jejuni and 43 genotypes for c. coli; ... | 2008 | 18648064 |
| genotypic and phenotypic diversity within three campylobacter populations isolated from broiler ceca and carcasses. | the main aim of this study was to trace campylobacter subtypes colonizing italian broilers and carcasses in and between flocks. overall, 209 campylobacter isolates were collected from ceca (n = 94) and carcasses (n = 115) of broilers belonging to 3 different flocks reared in the same farm during subsequent rotations and processed in the same slaughterhouse. all isolates were identified by multiplex polymerase chain reaction and genotyped by amplified fragment length polymorphism. furthermore, 16 ... | 2008 | 18809880 |
| campylobacter and arcobacter species sensitivity to commercial orange oil fractions. | seven orange oil fractions were screened for their ability to inhibit the growth of selected campylobacter and arcobacter spp. using the standard agar-disk diffusion assay. cold pressed (cp) terpeneless valencia orange oil was found to be the most inhibitory to both campylobacter jejuni and campylobacter coli, exhibiting maximum zones of inhibition up to 80+/-0.0 mm. five-fold concentrated valencia oil and distilled d-limonene resulted in campylobacter inhibition zones ranging from 11.0+/-1.4 to ... | 2009 | 19070381 |
| [incidence of the virulence markers iam in campylobacter jejuni and campylobacter coli strains isolated from poultry carcases]. | previously found dna sequence (iam), correlated with the invasiveness of campylobacter strains, became a starting point for present investigation. main goal of this study was to isolate number of campylobacter strains from chicken carcasses, to determine their taxonomic position and to establish the presence of iam sequence in their genoms. it was found, that invasion associated marker is present in all campylobacter coli strains and in majority but not all campylobacter jejuni. this may confirm ... | 2004 | 19097580 |
| antimicrobial resistance in selected bacteria from poultry. | this paper reviews the present state of antimicrobial resistance (amr) in the zoonotic bacteria salmonella, campylobacter jejuni and campylobacter coli, and in escherichia coli from chickens and turkeys. for salmonella, the frequencies and patterns of amr vary depending on time, region, serovar, the particular farm, layers versus broilers, and the antimicrobial agent. there is usually a higher frequency of amr in salmonella from turkeys compared with salmonella from chickens. clonal and horizont ... | 2008 | 19102788 |
| comparison of loop-mediated isothermal amplification assay and conventional culture methods for detection of campylobacter jejuni and campylobacter coli in naturally contaminated chicken meat samples. | we investigated the efficacy of a loop-mediated isothermal amplification (lamp) assay for detection of chicken meat samples naturally contaminated with campylobacter jejuni and campylobacter coli. a total of 144 preston enrichment broth cultures from chicken meat samples were assessed by using the lamp assay and conventional culture methods, which consist of a combination of preston enrichment culturing and plating onto butzler and modified charcoal cefoperazone deoxycholate agars. compared with ... | 2009 | 19139242 |
| occurrence and characterization of campylobacter in the brazilian production and processing of broilers. | twenty-two commercial broiler flocks and their carcasses, totaling 546 samples (450 collected from a poultry farm and 96 from a slaughterhouse), were surveyed for the presence of campylobacter. the positive results for campylobacter among the analyzed samples were homogeneous, yielding 81.8% for cecal droppings, 80.9% for feces, and 80.4% for cloacal swabs. pre-enrichment and direct plating showed that 77.85% and 81.8% of cloacal swabs, respectively, were positive for campylobacter compared to 9 ... | 2008 | 19166063 |
| thermophilic campylobacter spp. in danish broiler production: a cross-sectional survey and a retrospective analysis of risk factors for occurrence in broiler flocks. | in order to elucidate the rate of thermophilic campylobacter spp. carriage in danish broiler production and to identify risk factors for occurrence of campylobacter in broiler flocks, a total of 88 randomly selected broiler flocks were tested for campylobacter infection, and a subsequent study of risk factors based on a questionnaire was conducted. the sample material comprised cloacal swabs from live birds before slaughter, and neck skin samples from carcasses at the end of the processing line. ... | 2000 | 19184798 |
| genetic diversity of campylobacter on broiler carcasses collected preevisceration and postchill in 17 u.s. poultry processing plants. | campylobacter jejuni and campylobacter coli are the most important human enteropathogens among the campylobacters. the objective of this study was to determine how diversity in campylobacter populations found on chicken carcasses collected from 17 broiler processing plants in the united states is impacted by processing. genetic diversity was determined for up to four isolates per carcass by sequencing the short variable region (svr) of the flaa locus. on 70% of campylobacter-positive carcasses, ... | 2009 | 19205463 |
| a pan-european survey of antimicrobial susceptibility towards human-use antimicrobial drugs among zoonotic and commensal enteric bacteria isolated from healthy food-producing animals. | the aim of the study was to study antimicrobial susceptibility in escherichia coli, salmonella, campylobacter and enterococcus recovered from chickens, pigs and cattle using uniform methodology. | 2009 | 19233897 |
| campylobacter genotypes from food animals, environmental sources and clinical disease in scotland 2005/6. | a nationwide multi-locus sequence typing (mlst) survey was implemented to analyze patterns of host association among campylobacter jejuni and campylobacter coli isolates from clinical disease in scotland (july 2005-september 2006), food animals (chickens, cattle, sheep, pigs and turkey), non-food animals (wild birds) and the environment. sequence types (sts) were determined for 5247 clinical isolates and 999 from potential disease sources (augmented with 2420 published sts). certain sts were ove ... | 2009 | 19269051 |
| campylobacter genotyping to determine the source of human infection. | campylobacter species cause a high proportion of bacterial gastroenteritis cases and are a significant burden on health care systems and economies worldwide; however, the relative contributions of the various possible sources of infection in humans are unclear. | 2009 | 19275496 |
| survival at refrigeration and freezing temperatures of campylobacter coli and campylobacter jejuni on chicken skin applied as axenic and mixed inoculums. | campylobacter is considered to be the most common cause of bacterial diarrhoeal illness in the developed world. many cases are thought to be acquired from consumption of undercooked poultry. the aim of this study was to compare the effect of the rate of cooling on the survival, at 4 degrees c and -20 degrees c, of campylobacter coli and campylobacter jejuni strains, inoculated on chicken skin from axenic culture or as mixed inoculums. strains chilled in a domestic refrigerator varied in their to ... | 2009 | 19324444 |
| application of a group ii campylobacter bacteriophage to reduce strains of campylobacter jejuni and campylobacter coli colonizing broiler chickens. | members of the genus campylobacter are frequently responsible for human enteric disease worldwide. persistent campylobacter contamination of poultry meat is a common problem that represents a significant food safety risk through the consumption of undercooked poultry meat or through cross-contamination of other foods during the preparation of poultry. bacteriophage therapy is one possible means by which this colonization of poultry could be controlled, thus limiting the entry of campylobacter in ... | 2009 | 19435220 |
| survival rate of thermotolerant campylobacter on poultry meat during microwave heating. | survival rates of campylobacterjejuni atcc 33 291, campylobacter jejuni pzh 38 and campylobacter coli atcc 43 478 in leg quarters (ca 330 g) of chicken broilers after microwave heating (480 and 760 watt) for 2, 4, 6, 8 and 10 minutes were determined. heating the meat in a microwave oven (moulinex, type dialog cook) at 480 watts caused a gradual decrease in the number of cells of the studied bacterial strains to total inactivation after 8-10 minutes of heating. increasing the heating power to 760 ... | 2009 | 19459438 |
| dna identification and characterization of campylobacter jejuni and campylobacter coli isolated from caecal samples of chickens in grenada. | to speciate campylobacter strains from the caeca of chickens in grenada using pcr and to evaluate dna-based typing methods for the characterization of these isolates. | 2010 | 19735321 |
| cost analysis and antimicrobial susceptibility testing comparing the e test and the agar dilution method in campylobacter jejuni and campylobacter coli. | although numerous reports have compared the antimicrobial susceptibility of campylobacter spp., controversy still exists about the use of the e test as an alternative to the agar dilution method suggested by the clinical and laboratory standards institute. mics of 8 antimicrobials were determined using the e test and agar dilution methods for 103 campylobacter jejuni and campylobacter coli isolates from fresh chicken randomly purchased from stores in 3 southern ontario counties. overall, 72.6% o ... | 2009 | 19748428 |
| mouse intestine colonization ability of campylobacter coli strains. | campylobacter coli is an etiological agent of gastrointestinal and extraintestinal infections in man and animals, and can be found as a commensal in gastrointestinal tract of animals. in this study, we aimed to determine differences among c coli strains in colonization of the intestinal tract of mice. seven c coli strains isolated from diarrheic patients, asymptomatic hosts and chicken carcasses were used for this study. each strain was inoculated with 0.1 ml of a bacterial suspension (3 x 10(8) ... | 2009 | 19753795 |
| prevalence of campylobacter spp. in raw retail poultry on sale in northern ireland. | a year-long survey of fresh, retail poultry products on sale in northern ireland was undertaken to define the prevalence of campylobacter spp. by using protocols based on iso (standard) 10272-1:2006. incubation at 37 and 42 degrees c was undertaken to increase the diversity of isolates obtained. overall, 652 isolates were identified as campylobacter spp. by using pcr and amplified fragment length polymorphic typing. phenotyping wrongly identified 21% of isolates. prevalences of campylobacter fou ... | 2009 | 19777882 |
| development and evaluation of internal amplification controls for use in a real-time duplex pcr assay for detection of campylobacter coli and campylobacter jejuni. | a common problem of both conventional and real-time pcr assays is failure of dna amplification due to the presence of inhibitory substances in samples. in view of this, our aim was to develop and evaluate internal amplification controls (iacs) for use with an existing duplex real-time pcr assay for campylobacter coli and campylobacter jejuni. both competitive and non-competitive iacs were developed and evaluated. the competitive approach involved a dna fragment of the coding region of the fish v ... | 2010 | 19833779 |
| campylobacter species occurrence within internal organs and tissues of commercial caged leghorn laying hens. | campylobacter spp. are frequently present in the intestinal tract and internal tissues of broiler breeder and broiler chickens. campylobacter spp. ecology in commercial leghorn laying hens has not been extensively studied. the objectives of the current study were to determine 1) campylobacter spp. presence in the reproductive tract, lymphoid organs, liver-gallbladder, and ceca of commercial leghorn laying hens; 2) species of campylobacter present; and 3) antimicrobial resistance pattern of campy ... | 2009 | 19834099 |
| genetic similarity of campylobacter isolates in humans, food, and water sources in central poland. | campylobacter spp. is an important cause of gastroenteritis in humans throughout the world. however, sources of these infections are often difficult to identify. therefore, this study aimed at analyzing the genetic relatedness of campylobacter isolates from environmental and food samples as well as stool specimens of diarrheal patients obtained in a single geographic region in poland. only a few campylobacter jejuni isolates (4/18, 22%) could be assigned to one cluster, whereas the majority were ... | 2010 | 20001328 |
| method for bacteriophage isolation against target campylobacter strains. | poultry meat is considered a major source of campylobacter. this micro-aerobic bacterium is commonly responsible for foodborne illness. this work focuses on the isolation of campylobacter coli lytic bacteriophages (phages) against target c. coli strains. | 2010 | 20002571 |
| easy-to-use rapid test for direct detection of campylobacter spp. in chicken feces. | human campylobacteriosis is the leading cause of acute bacterial gastroenteritis in developed countries. one important source of infection is poultry. results from the dutch campylobacter risk management and assessment project indicate that meat from broiler flocks shedding >or=7 log cfu campylobacter per g of feces poses the greatest risk of transmitting campylobacteriosis. the objective of this study was to develop a simple and rapid test that would identify chicken flocks shedding high number ... | 2009 | 20003729 |
| expanded multilocus sequence typing and comparative genomic hybridization of campylobacter coli isolates from multiple hosts. | the purpose of this work was to evaluate the evolutionary history of campylobacter coli isolates derived from multiple host sources and to use microarray comparative genomic hybridization to assess whether there are particular genes comprising the dispensable portion of the genome that are more commonly associated with certain host species. genotyping and clonalframe analyses of an expanded 16-gene multilocus sequence typing (mlst) data set involving 85 isolates from 4 different hosts species te ... | 2010 | 20097815 |
| prevalence and antimicrobial resistance of campylobacter isolates in broilers from china. | the prevalence and antimicrobial resistance of campylobacter spp. in broiler chickens were determined in shandong province, china. in total, 275 campylobacter isolates were obtained from 767 broiler cecal samples, including 208 campylobacter jejuni, 53 campylobacter coli, and 14 unidentified campylobacter isolates. minimal inhibitory concentrations of 11 antimicrobial agents were determined using the agar dilution method recommended by clsi. more than 98% of the tested campylobacter isolates wer ... | 2010 | 20116182 |
| a pulsed-field gel electrophoresis study of the genetic diversity of campylobacter jejuni and campylobacter coli in poultry flocks in slovenia. | campylobacter jejuni and c. coli have recently become the most frequent cause of bacterial foodborne enteric infection in most industrialised countries. consumption and handling of undercooked contaminated poultry meat was identified as an important risk factor for human campylobacteriosis. the aim of this study was to ascertain the genetic diversity of c. jejuni and c. coli strains isolated from poultry in slovenia. a total of 68 isolates (42 c. jejuni , 26 c. coli ) from faeces (n = 48), meat ... | 2010 | 20159735 |
| resistance to antimicrobial agents of campylobacter spp. strains isolated from animals in poland. | a total of 69 campylobacter jejuni and 16 campylobacter coli strains isolated from chicken, dog and pig stool samples were characterized based on their resistance to five antimicrobial agents and on plasmid ptet profiles. antimicrobials used in this study were: amoxicillin/clavulanic acid, ciprofloxacin, erythromycin, tetracycline and trimethoprim/sulfamethoxazole. among the isolates studied, 91.7% were resistant to one or more antimicrobial agent. the highest level of resistance for the whole t ... | 2009 | 20169919 |
| effects of polyphosphate additives on campylobacter survival in processed chicken exudates. | campylobacter spp. are responsible for a large number of the bacterial food poisoning cases worldwide. despite being sensitive to oxygen and nutritionally fastidious, campylobacter spp. are able to survive in food processing environments and reach consumers in sufficient numbers to cause disease. to investigate campylobacter persistence on processed chicken, exudates from chickens produced for consumer sale were collected and sterilized. two types of exudates from chicken products were collected ... | 2010 | 20173061 |
| reduction of campylobacter jejuni and campylobacter coli in poultry skin by fruit extracts. | campylobacter spp. are a major cause of foodborne bacterial gastroenteritis in humans, and current methods to control campylobacter contamination in foods are not completely successful. plants are a promising source of antimicrobial agents, particularly given the growing interest in "all natural" foods. in this study, the antimicrobial activity of extracts from 28 edible plants against campylobacter jejuni and campylobacter coli was evaluated in vitro and in a poultry skin model. nine of 28 extr ... | 2010 | 20202332 |
| risk factors for indigenous campylobacter jejuni and campylobacter coli infections in the netherlands: a case-control study. | a case-control study comprising 1315 campylobacter jejuni cases, 121 campylobacter coli cases and 3409 frequency-matched controls was conducted in the netherlands in 2002-2003. risk factors for both c. jejuni and c. coli enteritis were consumption of undercooked meat and barbecued meat, ownership of cats and use of proton pump inhibitors. consumption of chicken was a predominant risk factor for c. jejuni enteritis, but many additional risk factors were identified. unique risk factors for c. coli ... | 2010 | 20223048 |
| in vitro susceptibility to antimicrobial agents and ultrastructural characteristics related to swimming motility and drug action in campylobacter jejuni and c. coli. | campylobacter jejuni has recently been noted as the most common cause of bacterial food-borne diseases in japan. in this study, we examined in vitro susceptibility to 36 antimicrobial agents of 109 strains of c. jejuni and c. coli isolated from chickens and patients with enteritis or guillain-barré syndrome from 1996 to 2009. among these agents, carbapenems (imipenem, meropenem, panipenem, and biapenem) showed the greatest activity [minimal inhibitory concentration (mic)(90), 0.03-0.125 microg/m ... | 2010 | 20225076 |
| antimicrobial resistance, virulence, and genotypic profile comparison of campylobacter jejuni and campylobacter coli isolated from humans and retail meats. | a total of 360 spatially and temporally related campylobacter isolates, including 168 from clinical human cases (campylobacter jejuni n = 148; campylobacter coli n = 20) and 192 from retail meats (c. jejuni n = 114; c. coli n = 78), were analyzed for antimicrobial susceptibilities, virulence, and genotypic profiles. ciprofloxacin-resistant c. jejuni was observed in 13.5% and 19% of the isolates from humans and retail chicken breasts, respectively. antimicrobial resistance to ciprofloxacin and er ... | 2010 | 20367499 |
| prevalence and antimicrobial resistance of campylobacter species isolated from chicken carcasses during processing in iran. | the objective of this study was to determine the prevalence and antimicrobial resistance of campylobacter spp. isolated from chicken carcasses during different stages of broiler processing in a major commercial poultry processing plant in southwestern iran. overall, 84 chicken carcasses were sampled from 4 sites along the processing line during a total of 7 visits. in addition, 14 water samples from the chiller tank were taken. using the cultural method, 186 of 336 (55.4%) carcasses were positiv ... | 2010 | 20371855 |
| prevalence and biomolecular characterization of campylobacter spp. isolated from retail meat. | we estimated the prevalence of campylobacter spp. in retail meat (n = 352 samples; 104 chicken, 106 pork, and 142 beef) collected in campobasso, italy, comparing two microbiological methods. all the isolates were characterized by biomolecular techniques for epidemiological purposes. campylobacter isolation was performed by selective culture and membrane filtration methods. phenotypic and genotypic methods for genus and species identification were evaluated together with antimicrobial resistance ... | 2010 | 20377962 |
| prevalence and antimicrobial resistance of campylobacter isolates from humans and chickens in bahir dar, ethiopia. | in this study, the isolation and antimicrobial resistance of campylobacter jejuni and campylobacter coli strains from chickens and humans in bahir dar, ethiopia, were analyzed. two hundred and ten human and 220 chicken samples were analyzed between october 2007 and april 2008. seventeen human and 160 chicken campylobacter species were isolated. the overall prevalence of thermophilic campylobacters was 8% and 72.7% in humans and chickens, respectively. in humans, 94.1% of the isolates were c. jej ... | 2010 | 20482228 |
| genotyping and pcr detection of potential virulence genes in campylobacter jejuni and campylobacter coli isolates from different sources in poland. | the prevalence of potential virulence markers was determined among the population of polish campylobacter jejuni and campylobacter coli isolates from children, chickens, pigs and dogs. the presence of the flaa, flab, cdta, cdtb, cdtc, cdtabc, virb11, and cj0588 genes among 74 c. jejuni and 15 c. coli isolates was detected by pcr. high prevalence of five different putative virulence and toxin genes (flaa, cdta, cdtb, cdtc, and cj0588) was found among isolates obtained from children, chickens and ... | 2010 | 20490760 |
| host association of campylobacter genotypes transcends geographic variation. | genetic attribution of bacterial genotypes has become a major tool in the investigation of the epidemiology of campylobacteriosis and has implicated retail chicken meat as the major source of human infection in several countries. to investigate the robustness of this approach to the provenance of the reference data sets used, a collection of 742 campylobacter jejuni and 261 campylobacter coli isolates obtained from united kingdom-sourced chicken meat was established and typed by multilocus seque ... | 2010 | 20525862 |
| molecular typing of campylobacter jejuni and c. coli from chickens and patients with gastritis or guillain-barré syndrome based on multilocus sequence types and pulsed-field gel electrophoresis patterns. | campylobacter jejuni has recently been noted as the most common cause of bacterial foodborne diseases in japan. in the present study, we determined st types of c. jejuni and campylobacter coli isolated from chickens and patients with enteritis or gbs in japan and thailand. c. jejuni from chickens, enteritis, and gbs exhibited divergent st types and included several novel types in addition to worldwide common types. c. coli from enteritis was also divergent. novel st types may represent unidentif ... | 2010 | 20536735 |
| fluoroquinolone resistance in campylobacter. | campylobacter is a commensal in poultry, and therefore, poultry and poultry products are major sources of campylobacter infections in humans. fluoroquinolones inhibit the growth of campylobacter and other microorganisms by binding to bacterial dna gyrase and dna topoisomerase iv. these enzymes are associated with bacterial transcription, replication, and chromosome condensation and segregation. selection pressure in the presence of fluoroquinolones rapidly leads to resistance in campylobacter, d ... | 2010 | 20537275 |
| identification and characterization of a new ferric enterobactin receptor, cfrb, in campylobacter. | the ferric enterobactin (feent) receptor cfra is present in the majority of campylobacter jejuni isolates and is responsible for high-affinity iron acquisition. our recent work and that of others strongly suggested the existence of another feent uptake system in campylobacter. here we have identified and characterized a new feent receptor (designated cfrb) using both in vitro and in vivo systems. cfrb, a homolog of c. jejuni nctc 11168 cj0444, shares approximately 34% of amino acid identity with ... | 2010 | 20585060 |
| campylobacter spp among children with acute diarrhea attending mulago hospital in kampala--uganda. | campylobacter infections occur worldwide. a recent study in kampala, uganda, found that 87% of broiler chickens had campylobacter jejuni; these are potential source of human infection. isolation rate in developing countries is between 5-35%. this study aimed at finding prevalence of children with campylobacter infection among children with acute diarrhea attending mulago hospital. | 2009 | 20589152 |
| prevalence and antimicrobial resistance in campylobacter spp. isolated from retail chicken in two health units in ontario. | campylobacter is an important enteric pathogen of humans and can cause diarrhea, fever, and abdominal pain. campylobacter infections have frequently been associated with the handling and consumption of raw and undercooked poultry. antimicrobial resistance among campylobacter strains is of concern in the treatment of campylobacteriosis in vulnerable populations. a 2-year multidisciplinary study was conducted in the perth and wellington-dufferin-guelph public health units in ontario, canada, to in ... | 2010 | 20615345 |
| simple and rapid detection of campylobacter spp. in naturally contaminated chicken-meat samples by combination of a two-step enrichment method with an immunochromatographic assay. | a simple and rapid method to detect campylobacter spp. in chicken-meat samples was established. this method consisted of a combination of a two-step enrichment method with a commercially available immunochromatographic assay, named nh immunochromato campylobacter (nh ic campy, nippon meat packers, ibaraki, japan), which is able to detect campylobacter antigen in an enrichment culture within 15 min. the enrichment method did not require much blood or a particular system of generating a microaerob ... | 2010 | 20621373 |
| the in vivo efficacy of two administration routes of a phage cocktail to reduce numbers of campylobacter coli and campylobacter jejuni in chickens. | poultry meat is one of the most important sources of human campylobacteriosis, an acute bacterial enteritis which is a major problem worldwide. campylobacter coli and campylobacter jejuni are the most common campylobacter species associated with this disease. these pathogens live in the intestinal tract of most avian species and under commercial conditions they spread rapidly to infect a high proportion of the flock, which makes their treatment and prevention very difficult. bacteriophages (phag ... | 2010 | 20809975 |