Publications

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recent laboratory contributions to the control of yellow fever: (section of tropical diseases and parasitology).the most important recent laboratory contributions to the control of yellow fever will be briefly summarized under three headings: (1) methods of diagnosis, (2) transmission, and (3) protection.(1) methods of diagnosis.-the development of improved methods of identification, in particular by immunity tests, has made it possible to diagnose yellow fever with much greater certainty. moreover, since the immunity following an attack of the disease is usually of life-long duration, it is possible to d ...193319989606
interaction of flavivirus with their mosquito vectors and their impact on the human health in the americas.some of the major arboviruses with public health importance, such as dengue, yellow fever, zika and west nile virus are mosquito-borne or mosquito-transmitted flavivirus. their principal vectors are from the family culicidae, aedes aegypti and aedes albopictus being responsible of the urban cycles of dengue, zika and yellow fever virus. these vectors are highly competent for transmission of many arboviruses. the genetic variability of the vectors, the environment and the viral diversity modulate ...201728499872
potential risk of re-emergence of urban transmission of yellow fever virus in brazil facilitated by competent aedes populations.yellow fever virus (yfv) causing a deadly viral disease is transmitted by the bite of infected mosquitoes. in brazil, yfv is restricted to a forest cycle maintained between non-human primates and forest-canopy mosquitoes, where humans can be tangentially infected. since late 2016, a growing number of human cases have been reported in southeastern brazil at the gates of the most populated areas of south america, the atlantic coast, with rio de janeiro state hosting nearly 16 million people. we sh ...201728687779
the multiplication of the virus of yellow fever in aedes aegypti.aëdes aegypti have been shown to be capable of multiplying the asibi strain of yellow fever virus in their bodies. following the ingestion of infected blood, the content of virus falls for several days, reaching a minimum during the 1st week. it then increases rapidly until quantities of virus greater than those previously encountered can be demonstrated. the actual final amount of virus demonstrable, however, is subject to variations of which we know little.193719870651
cell fusing agent virus and dengue virus mutually interact in aedes aegypti cell lines.the genus flavivirus contains more than 70 single-stranded, positive-sense arthropod-borne rna viruses. some flaviviruses are particularly medically important to humans and other vertebrates including dengue virus (denv), west nile virus, and yellow fever virus. these viruses are transmitted to vertebrates by mosquitoes and other arthropod species. mosquitoes are also infected by insect-specific flaviviruses (isfs) that do not appear to be infective to vertebrates. cell fusing agent virus (cfav) ...201728761113
assessment of risk of dengue and yellow fever virus transmission in three major kenyan cities based on stegomyia indices.dengue (den) and yellow fever (yf) are re-emerging in east africa, with contributing drivers to this trend being unplanned urbanization and increasingly adaptable anthropophilic aedes (stegomyia) vectors. entomological risk assessment of these diseases remains scarce for much of east africa and kenya even in the dengue fever-prone urban coastal areas. focusing on major cities of kenya, we compared den and yf risk in kilifi county (den-outbreak-prone), and kisumu and nairobi counties (no document ...201728817563
the single kinin receptor signals to separate and independent physiological pathways in malpighian tubules of the yellow fever mosquito.in the past, we have used the kinins of the cockroach leucophaea (the leucokinins) to evaluate the mechanism of diuretic action of kinin peptides in malpighian tubules of the yellow fever mosquito aedes aegypti. now using the kinins of aedes (the aedeskinins), we have found that in isolated aedes malpighian tubules all three aedeskinins (1 microm) significantly 1) increased the rate of fluid secretion (v(s)), 2) hyperpolarized the basolateral membrane voltage (v(bl)), and 3) decreased the input ...201020538895
incubation periods of yellow fever virus.yellow fever virus is a global health threat due to its endemicity in parts of africa and south america where human infections occur in residents and travelers. to understand yellow fever dynamics, it is critical to characterize the incubation periods of the virus in vector mosquitoes and humans. here, we compare four statistical models of the yellow fever incubation periods fitted with historical data. the extrinsic incubation period in the urban vector aedes aegypti was best characterized with ...201020595499
a list of mosquito species of the brazilian state of pernambuco, including the first report of haemagogus janthinomys (diptera: culicidae), yellow fever vector and 14 other species (diptera: culicidae).besides mosquito species adapted to urban environments (culex quinquefasciatus, aedes aegypti and aedes albopictus), only 15 species of anopheles had been recorded in the state of pernambuco.201020802950
early history of laboratory breeding of aedes aegypti (diptera: culicidae) focusing on the origins and use of selected strains.the yellow fever mosquito, aedes aegypti (l.) (diptera: culicidae), is well recognized for its extensive adaptation to diverse ecological conditions and for genetic variation. recognizing the importance of strain variation of this mosquito, researchers have established a large number of laboratory strains. some of the popular strains have been used for research for years in many laboratories around the world. however, the exact origins of many of these strains are unknown. in this review, public ...201021175042
[entomological investigations conducted around ten cases of yellow fever in 2009 in the denguélé sanitary region, côte-d'ivoire.]in november 2009, ten suspicious cases of yellow fever, including six deaths, were notified in the region of denguélé, in the northwest of côte-d'ivoire. in order to evaluate the extent of yellow fever virus circulation and the risk for local people, a mission of entomological investigation was carried out by the ministry of health and public hygiene of côte-d'ivoire. entomological investigations were conducted in the villages of confirmed cases (banakoro and tron-touba) and the centers of consu ...201121293956
a protocol for collecting and staining hemocytes from the yellow fever mosquito <em>aedes aegypti</em>.mosquitoes are vectors for a number of disease-causing pathogens such as the yellow fever virus, malaria parasites and filarial worms. laboratories are investigating anti-pathogen components of the innate immune system in disease vector species in the hopes of generating transgenic mosquitoes that are refractory to such pathogens(1, 2). the innate immune system of mosquitoes consists of several lines of defense (3). pathogens that manage to escape the barrier imposed by the epithelium-lined mosq ...201121633325
vector competence of australian mosquitoes for yellow fever virus.the vector competence of australian mosquitoes for yellow fever virus (yfv) was evaluated. infection and transmission rates in cairns and townsville populations of aedes aegypti and a brisbane strain of ae. notoscriptus were not significantly different from a well-characterized yfv-susceptible strain of ae. aegypti. after exposure to 10⁷·² tissue culture infectious dose (tcid₅₀)/ml of an african strain of yfv, > 70% of ae. aegypti and ae. notoscriptus became infected, and > 50% transmitted the v ...201121896802
development of a sybr green i based rt-pcr assay for yellow fever virus: application in assessment of yfv infection in aedes aegypti.yellow fever virus (yfv) is an important arboviral pathogen in much of sub-saharan africa and the tropical americas. it is the prototype member of the genus flavivirus and is transmitted primarily by aedes (stegomyia) mosquitoes. the incidence of human infections in endemic areas has risen in recent years. prompt and dependable identification of yfv is a critical component of response to suspect cases.201222264275
is there a risk of yellow fever virus transmission in south asian countries with hyperendemic dengue?the fact that yellow fever (yf) has never occurred in asia remains an "unsolved mystery" in global health. most countries in asia with high aedes aegypti mosquito density are considered "receptive" for yf transmission. recently, health officials in sri lanka issued a public health alert on the potential spread of yf from a migrant group from west africa. we performed an extensive review of literature pertaining to the risk of yf in sri lanka/south asian region to understand the probability of ac ...201324367789
fever versus fever: the role of host and vector susceptibility and interspecific competition in shaping the current and future distributions of the sylvatic cycles of dengue virus and yellow fever virus.two different species of flaviviruses, dengue virus (denv) and yellow fever virus (yfv), that originated in sylvatic cycles maintained in non-human primates and forest-dwelling mosquitoes have emerged repeatedly into sustained human-to-human transmission by aedes aegypti mosquitoes. sylvatic cycles of both viruses remain active, and where the two viruses overlap in west africa they utilize similar suites of monkeys and aedes mosquitoes. these extensive similarities render the differences in the ...201323523817
bicluster pattern of codon context usages between flavivirus and vector mosquito aedes aegypti: relevance to infection and transcriptional response of mosquito genes.the mosquito aedes aegypti is the primary vector of dengue virus (denv) infection in most of the subtropical and tropical countries. besides denv, yellow fever virus (yfv) is also transmitted by a. aegypti. susceptibility of a. aegypti to west nile virus (wnv) has also been confirmed. although studies have indicated correlation of codon bias between flaviviridae and their animal/insect hosts, it is not clear if codon sequences have any relation to susceptibility of a. aegypti to denv, yfv and wn ...201424838953
spatio-temporal distribution of aedes aegypti (diptera: culicidae) mitochondrial lineages in cities with distinct dengue incidence rates suggests complex population dynamics of the dengue vector in colombia.aedes aegypti is the primary vector of the four serotypes of dengue virus (denv1-4), chikungunya and yellow fever virus to humans. previous population genetic studies have revealed a particular genetic structure among the vector populations in the americas that suggests differences in the ability to transmit denv. in colombia, despite its high epidemiologic importance, the genetic population structure and the phylogeographic depiction of ae. aegypti, as well as its relationship with the epidemio ...201525893246
microhabitat partitioning of aedes simpsoni (diptera: culicidae).yellow fever virus is a reemerging infection responsible for widespread, sporadic outbreaks across africa. although aedes aegypti (l.) is the most important vector globally, in east africa, epidemics may be vectored by aedes bromeliae (theobald), a member of the aedes simpsoni (theobald) species complex. the ae. simpsoni complex contains 10 subspecies, of which ae. bromeliae alone has been incriminated as a vector of yellow fever virus. however, morphological markers cannot distinguish ae. brome ...024897852
blood meal induced microrna regulates development and immune associated genes in the dengue mosquito vector, aedes aegypti.aedes aegypti is a blood-feeding mosquito that transmits human pathogens such as dengue virus, yellow fever virus and chikungunya virus. recently, dramatic changes in the transcriptome of ae. aegypti following a blood meal have been reported; however, the molecular factors involved in regulating these changes are largely unknown. in this study, we found induction of a number of endogenous micrornas (mirnas) in blood fed (bf) mosquitoes. one of these mirnas, aae-mir-375, was only detected in bf m ...201323202267
infection of mosquito cells (c6/36) by dengue-2 virus interferes with subsequent infection by yellow fever virus.dengue is one of the most important diseases caused by arboviruses in the world. yellow fever is another arthropod-borne disease of great importance to public health that is endemic to tropical regions of africa and the americas. both yellow fever and dengue viruses are flaviviruses transmitted by aedes aegypti mosquitoes, and then, it is reasonable to consider that in a given moment, mosquito cells could be coinfected by both viruses. therefore, we decided to evaluate if sequential infections o ...201626808727
natural transovarial transmission of dengue virus 4 in aedes aegypti from cuiabá, state of mato grosso, brazil.dengue is the most prevalent arboviral disease in tropical areas. in mato grosso, outbreaks are reported every year, but studies on dengue in this state are scarce.201525860459
diversity of yellow fever mosquito vectors in the atlantic forest of rio de janeiro, brazil.environmental modifications caused by human activities have led to changes in mosquito vector populations, and sylvatic species have adapted to breeding in urban areas.201727384833
effects of proteinase inhibitor from adenanthera pavonina seeds on short- and long term larval development of aedes aegypti.currently, one of the major global public health concerns is related to the transmission of dengue/yellow fever virus by the vector aedes aegypti. the most abundant digestive enzymes in ae. aegypti midgut larvae are trypsin and chymotrypsin. since protease inhibitors have the capacity to bind to and inhibit the action of insect digestive proteinases, we investigated the short- and long-term effects of adenanthera pavonina seed proteinase inhibitor (apti) on ae. aegypti larvae, as well as a possi ...201525796215
[management of the yellow fever epidemic in 2010 in séguéla (côte d'ivoire): value of multidisciplinary investigation].in august 2010, five positive cases of yellow fever were reported in the region of séguéla, in the northwest of côte d'ivoire, affected by an armed conflict since 2002. in order to evaluate the extent of yellow fever virus circulation and the risk for local people, a multidisciplinary investigation was carried out by the côte-d'ivoire ministry of health and public hygiene.201525629680
[yellow fever: reemerging in the state of sao paulo, brazil, 2009].to describe the investigation of a sylvatic yellow fever outbreak in the state of sao paulo and the main control measures undertaken.201324626492
yellow fever virus susceptibility of two mosquito vectors from kenya, east africa.yellow fever is an unpredictable disease of increasing epidemic threat in east africa. aedes (stegomyia) aegypti has never been implicated as a vector in this region and recent outbreaks have involved a newly emerging virus genotype (east african). to better understand the increasing epidemic risk of yellow fever in east africa, this study is the first to investigate the vector competence for an emerging east african virus genotype in kenyan a. aegypti sensu latu (s.l) and a. (stegomyia) simpson ...201222521217
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