| effect of antimicrobial and physical treatments on growth of multispecies staphylococcal biofilms. | the prevalence and structure of staphylococcus aureus and staphylococcus epidermidis within multispecies biofilms were found to depend sensitively on physical environment and antibiotic dosage. although these species commonly infect similar sites, such as orthopedic implants, little is known about their behavior in multispecies communities, particularly in response to treatment. this research establishes that s. aureus is much more prevalent than s. epidermidis when simultaneously seeded and gro ... | 2017 | 28411222 |
| anti-bacterial and anti-biofilm evaluation of thiazolopyrimidinone derivatives targeting the histidine kinase yycg protein of staphylococcus epidermidis. | staphylococcus epidermidis is one of the most important opportunistic pathogens in nosocomial infections. the main pathogenicity associated with s. epidermidis involves the formation of biofilms on implanted medical devices, biofilms dramatically decrease the efficacy of conventional antibiotics and the host immune system. this emphasizes the urgent need for designing novel anti-staphylococcal biofilm agents. based on the findings that compound 5, targeting the histidine kinase domain of s. epid ... | 2017 | 28408903 |
| inhibited bacterial adhesion and biofilm formation on quaternized chitosan-loaded titania nanotubes with various diameters. | titania nanotube-based local drug delivery is an attractive strategy for combating implant-associated infection. in our previous study, we demonstrated that the gentamicin-loaded nanotubes could dramatically inhibit bacterial adhesion and biofilm formation on implant surfaces. considering the overuse of antibiotics may lead to the evolution of antibiotic-resistant bacteria, we synthesized a new quaternized chitosan derivative (hydroxypropyltrimethyl ammonium chloride chitosan, hacc) with a 27% d ... | 2016 | 28773281 |
| phenotypic characterisation of coagulase-negative staphylococci isolated from blood cultures in newborn infants, with a special focus on staphylococcus capitis. | this swedish study determined which species of coagulase-negative staphylococci (cons) were found in neonatal blood cultures and whether they included staphylococcus capitis clones with decreased susceptibility to vancomycin. | 2017 | 28631328 |
| biofilm formation of methicillin-resistant coagulase negative staphylococci (mr-cons) isolated from community and hospital environments. | methicillin-resistant coagulase negative staphylococci (mr-cons) are the major cause of infectious diseases because of their potential ability to form biofilm and colonize the community or hospital environments. this study was designed to investigate the biofilm producing ability, and the presence of meca, icaad, bap and fnba genes in mr-cons isolates. the mr-cons used in this study were isolated from various samples of community environment and five wards of hospital environments, using mannito ... | 2017 | 28859149 |
| hamstring autografts are associated with a high rate of contamination in anterior cruciate ligament reconstruction. | to quantitatively evaluate the rate, type, and level of contamination of anterior cruciate ligament (acl) hamstring autografts after harvesting and preparation or dropping onto the operating room (or) floor. | 2017 | 28852791 |
| intracavitary and systemic daptomycin for successful treatment of a postpneumonectomy intrathoracic infection. | treatment of an infected postneumonectomy cavity is very difficult. we present a patient with an infection of a postneumonectomy cavity by staphylococcus epidermidis treated with local daptomycin for different dwell times, maintaining high antibiotic levels over minimum inhibitory concentration. clinical and microbiological cure were achieved successfully. | 2017 | 28848014 |
| effect of ultrasonic irradiation on bacterial biofilms. | the purpose of this study was to clarify the effect of ultrasonic irradiation on biofilm produced by staphylococcus epidermidis (s. epidermidis), which causes central venous catheter-related infections. | 2017 | 28836084 |
| broad targeting specificity during bacterial type iii crispr-cas immunity constrains viral escape. | crispr loci are a cluster of repeats separated by short "spacer" sequences derived from prokaryotic viruses and plasmids that determine the targets of the host's crispr-cas immune response against its invaders. for type i and ii crispr-cas systems, single-nucleotide mutations in the seed or protospacer adjacent motif (pam) of the target sequence cause immune failure and allow viral escape. this is overcome by the acquisition of multiple spacers that target the same invader. here we show that tar ... | 2017 | 28826839 |
| limitations in the use of psmγ, agr, rnaiii, and biofilm formation as biomarkers to define invasive staphylococcus epidermidis from chronic biomedical device-associated infections. | staphylococcus epidermidis is a common cause of biomedical device-associated infections. agr is the major quorum sensing system in staphylococci and regulates virulence factors. four agr-specificity groups exist in s. epidermidis, and chronic s. epidermidis infections are hypothesised to select for agr-negative phenotypes. therefore, we investigated s. epidermidis strains from prosthetic joint- and catheter-associated infections to establish i) whether an infection selects for an agr-negative ph ... | 2017 | 28826573 |
| identification of major sequence types among multidrug-resistant staphylococcus epidermidis strains isolated from infected eyes and healthy conjunctiva. | we examined the presence of virulence and antibiotic resistance genes, sccmec types and determined the genomic diversity among ocular s. epidermidis isolates (patients-23, healthy controls-29). pcr determined the presence of antibiotic resistance genes, virulence genes and sccmec types among all isolates. mlst and pfge determined the genomic relatedness among them. all isolates of s. epidermidis showed resistance to at least one class of antibiotics of which 48 isolates were multidrug resistant ... | 2017 | 28824564 |
| pathogenic mechanisms and host interactions in staphylococcus epidermidis device-related infection. | staphylococcus epidermidis is a permanent member of the normal human microbiota, commonly found on skin and mucous membranes. by adhering to tissue surface moieties of the host via specific adhesins, s. epidermidis is capable of establishing a lifelong commensal relationship with humans that begins early in life. in its role as a commensal organism, s. epidermidis is thought to provide benefits to human host, including out-competing more virulent pathogens. however, largely due to its capacity t ... | 2017 | 28824556 |
| [effect of sodium houttuyfonate in combination with erythromycin on luxs, agr/rnaⅲ of staphylococus epidermidis]. | quorum sensing of bacteria and its specific gene expression regulation have a very important role in bacterial biofilm formation. luxs and agr are the key regulatory genes in quorum sensing of staphylococcus epidermidis, and rna ⅲ is the effector molecule of agr system. in order to evaluate the effects of sodium houttuyfonate in combination with erythromycin on the transcription level of s. epidermidis, serial dilution method was used to determine the mic of sodium houttuyfonate, erythromycin an ... | 2017 | 28822159 |
| a comparative genomics study of staphylococcus epidermidis from orthopedic device-related infections correlated with patient outcome. | staphylococcus epidermidis has emerged as an important opportunistic pathogen causing orthopedic device-related infections (odris). this study investigated the association of genome variation and phenotypic features of the infecting s. epidermidis isolate with the clinical outcome of the infected patient. s. epidermidis isolates were collected from 104 patients with odri. their clinical outcome was evaluated, after an average of 26 months, as either "cured" or "not cured". the isolates were test ... | 2017 | 28794175 |
| [prophylactic effectiveness of vacuum-assisted closure for high-risk patients undergoing cardiovascular surgery through median sternotomy]. | vacuum-assisted closure(vac) therapy is mainly used for tissue defects. vac therapy can remove exudate that could impair the healing process. we applied vac therapy in patients considered at high risk of surgical site infection who underwent cardiovascular surgery via standard median sternotomy. risk factors included advanced heart failure, obesity, diabetes mellitus, steroid administration, immunosuppressant administration, and chronic renal failure, etc. vac therapy was used in 134 patients. o ... | 2017 | 28790237 |
| first detailed genetic characterization of the structural organization of type iii arginine catabolic mobile elements (acmes) harbored by staphylococcus epidermidis using whole genome sequencing. | the type iii arginine catabolic mobile element (acme) was detected in three staphylococcus epidermidis oral isolates recovered from separate patients (one healthy, one healthy with dental implants and one with periodontal disease) based on acme-arc operon and -opp3 operon directed pcr. these isolates were subjected to whole genome sequencing to characterize the precise structural organization of acme iii for the first time and also revealed that all three isolates were the same sequence type, st ... | 2017 | 28760894 |
| microbial communities and physicochemical properties of myeolchi jeotgal (anchovy jeotgal) prepared with different types of salts. | myeolchi jeotgals (mjs) were prepared with purified salt (ps), solar salt aged for 1 year (ss), and bamboo salt melted 3 times (bs) at 10% and 20% (w/w) concentrations, and fermented for 28 weeks at 15°c. bs mj showed higher ph, lower titratable acidities than other samples because of alkalinity of bamboo salt. lactic acid bacteria (lab) counts increased until 4-6 weeks and then decreased gradually, and not detected after 20 weeks from mjs with 10% salt. yeast counts of ps mjs were higher than t ... | 2017 | 28750506 |
| staphylococcus epidermidis: a major player in bacterial sepsis? | | 2017 | 28748707 |
| violacein antimicrobial activity on staphylococcus epidermidis and synergistic effect on commercially-available antibiotics. | the study aimed to assess whether violacein has antimicrobial activity on s. epidermidis and synergistically modulates the action of commercially-available antimicrobial drugs. | 2017 | 28744944 |
| contamination risk of synovial biopsy cultures in total hip arthroplasty: a prospective review of 100 cases. | cultures of deep synovial biopsies remain an important tool in diagnosing periprosthetic joint infection, a devastating complication following total hip arthroplasty (tha). recent reports of unexpected positive intraoperative cultures in aseptic revision arthroplasty, however, challenge the validity and interpretation of these cultures. the aim of this study was to evaluate the contamination risk of synovial biopsy cultures collected intraoperatively during primary tha of healthy subjects. | 2017 | 28731488 |
| highly enriched, controllable, continuous aerosol sampling using inertial microfluidics and its application to real-time detection of airborne bacteria. | we report a novel microfluidic technique for sampling of aerosols into liquids. the two-phase fluid, sampling air and collecting liquid, forms a stratified flow in the curved microchannel. by passing fluids through the curved region, the particles are transferred from air into the liquid phase by the particle centrifugal and drag forces. this microfluidic-based aerosol-into-liquid sampling system, called the microsampler, is driven by particle inertial differences. to evaluate the physical parti ... | 2017 | 28723191 |
| elevated levels of circulating cell-free dna and neutrophil proteins are associated with neonatal sepsis and necrotizing enterocolitis in immature mice, pigs and infants. | preterm infants are highly susceptible to late-onset sepsis (los) and necrotizing enterocolitis (nec), but disease pathogenesis and specific diagnostic markers are lacking. circulating cell-free dna (cfdna) and immune cell-derived proteins are involved in multiple immune diseases in adults but have not been investigated in preterm neonates. we explored the relation of circulating neutrophil-associated proteins and cfdna to los and/or nec. using a clinically relevant preterm pig model of spontane ... | 2017 | 28714327 |
| the influence of surface texture and wettability on initial bacterial adhesion on titanium and zirconium oxide dental implants. | this study aims to investigate bacterial adhesion on different titanium and ceramic implant surfaces, to correlate these findings with surface roughness and surface hydrophobicity, and to define the predominant factor for bacterial adhesion for each material. | 2017 | 28714053 |
| folliculitis caused by staphylococcus epidermidis? | | 2017 | 28712270 |
| bacterial colonization of penile prosthesis after its withdrawal due to mechanical failure. | prosthetic surgery to treat erectile dysfunction has a risk of infection of up to 3%, but this risk can increase to 18% when the surgery involves replacement. this increased risk of infection is attributed to the bacterial colonization of the prosthesis during the initial surgery. | 2017 | 28711311 |
| defining the metal specificity of a multifunctional biofilm adhesion protein. | the accumulation associated protein (aap) of staphylococcus epidermidis mediates intercellular adhesion events necessary for biofilm growth. this process depends upon zn(2+) -induced self-assembly of g5 domains within the b-repeat region of the protein, forming anti-parallel, intertwined protein "ropes" between cells. pleomorphism in the zn(2+) -coordinating residues was observed in previously solved crystal structures, suggesting that the metal binding site might accommodate other transition me ... | 2017 | 28707417 |
| different behavior and response of staphylococcus epidermidis and streptococcus pneumoniae to a ventriculoperitoneal shunt: an in vitro study. | pneumococcal shunt infection is a rare event. there is no consensus on the therapeutic management of this kind of shunt infection according to literature reviews, and it seems to be different from infection with staphylococcus epidermidis. we studied 2 shunted patients with pneumococcal meningitis, both of whom were treated with only antibiotics. the management of these cases seems to be different from that of shunt catheter infection due to these bacteria. we conducted a laboratory study to sho ... | 2017 | 28704823 |
| copper as an alternative antimicrobial coating for implants - an in vitro study. | to investigate osteoconductive and antimicrobial properties of a titanium-copper-nitride (ticun) film and an additional bonit(®) coating on titanium substrates. | 2017 | 28698836 |
| hydrophobin coating prevents staphylococcus epidermidis biofilm formation on different surfaces. | staphylococcus epidermidis is a significant nosocomial pathogen in predisposed hosts because of its capability of forming a biofilm on indwelling medical devices. the initial stage of biofilm formation has a key role in s. epidermidis abiotic surface colonization. recently, many strategies have been developed to create new anti-biofilm surfaces able to control bacterial adhesion mechanisms. in this work, the self-assembled amphiphilic layers formed by two fungal hydrophobins (vmh2 and pac3) have ... | 2017 | 28686037 |
| high staphylococcus epidermidis colonization and impaired permeability barrier in facial seborrheic dermatitis. | seborrheic dermatitis (sd) is a common inflammatory skin condition. the etiology is unclear, although overgrowth of malassezia on the skin has been suggested to cause sd. this study investigated whether colonization with staphylococcus plays a role in facial sd, which was not well addressed previously. | 2017 | 28685715 |
| draft genome sequence of staphylococcus epidermidis (winslow and winslow) evans (atcc 14990). | here, we report the draft genome sequence for the type strain staphylococcus epidermidis (winslow and winslow) evans (atcc 14990). the assembly consisted of 2,457,519 bp with an observed g+c content of 32.04%. thirty-seven contigs were produced, including two putative plasmids, with a 296.8× coverage and an n50 of 180,848 bp. | 2017 | 28684584 |
| changing epidemiology of coagulase-negative staphylococci in normal flora of skin. | coagulase negative staphylococci (cons) have recently emerged as important pathogens causing nosocomial blood stream infections. to evaluate the prevalence of cons in cutaneous normal flora, skin swabs were collected from voluntary blood donors and processed for culture and identification using matrix assisted laser desorption ionisation-time of flight (maldi-tof). cons were isolated from 96% of blood donors, most commonly staphylococcus hominis (86%), followed by staphylococcus epidermidis (22% ... | 2017 | 28681820 |
| the role of breast milk in the colonization of neonatal gut and skin with coagulase-negative staphylococci. | backgroundwe aimed to determine the genetic relatedness between staphylococcus epidermidis colonizing breast milk (bm) and bm-fed neonates during the first month of life.methodss. epidermidis was isolated from the stool and skin swabs of 20 healthy term and 49 preterm neonates hospitalized in the neonatal intensive care unit and from the bm of mothers once a week and typed by multilocus variable-number tandem-repeat analysis. virulence-related genes were determined by pcr.resultsthe gut (95%) an ... | 2017 | 28665928 |
| staphylococcus epidermidis lipoteichoic acid: exocellular release and ltas gene expression in clinical and commensal isolates. | staphylococcus epidermidis atcc12228 lipoteichoic acid (lta) inhibits tnfα production from keratinocytes that are activated with poly i:c. however, this effect has not been proven in clinical or commensal isolates. | 2017 | 28639932 |
| the microorganisms on nurses' and health care workers' uniforms in the intensive care units. | the objective of this study was to identify the types of microorganisms present on uniforms of nurses, physicians, respiratory therapist, students, and housekeepers in intensive care units (icus). a convenience sampling was used to recruit the participants ( n = 115) who work at military hospital in jordan. environmental cultures ( n = 305) were taken from the participants who were nurses, physicians, students, respiratory therapists, and housekeepers. the number of participating nurses was 58 ( ... | 2017 | 28627936 |
| short communication: β-lactam resistance and vancomycin heteroresistance in staphylococcus spp. isolated from bovine subclinical mastitis. | the use of antimicrobial agents has led to the emergence of resistant bacterial strains over a relatively short period. furthermore, staphylococcus spp. can produce β-lactamase, which explains the survival of these strains in a focus of infection despite the use of a β-lactam antibiotic. the aim of this study was to evaluate the resistance of staphylococcus spp. isolated from bovine subclinical mastitis to oxacillin and vancomycin (by minimum inhibitory concentration) and to detect vancomycin he ... | 2017 | 28624285 |
| graphene nanolayers as a new method for bacterial biofilm prevention: preliminary results. | biofilms are microbial communities of surface-attached cells embedded in a self-produced extracellular matrix. they have been found to play a role in a wide variety of infections, including catheter-related urinary tract and bloodstream infections, and, therefore remain a significant source of morbidity and mortality among the world's population. recently, much attention has been devoted to the prevention of biofilm formation on implant surfaces. nanomaterials such as graphene, characterized by ... | 2017 | 28623661 |
| antibiofilm effect of ultrasound combined with microbubbles against staphylococcus epidermidis biofilm. | biofilms are difficult to eradicate due to their resistance to antibiotics and host immune cells. ultrasound microbubbles have emerged as a new treatment modality with the underlying mechanisms largely unknown. in this study, we exposed 24-h-old staphylococcus epidermidis biofilms established in opticell™ chambers to ultrasound in combination with microbubbles, and investigated the activities of vancomycin and neutrophils against s. epidermidis biofilms after treatment. the antibiofilm mechanims ... | 2017 | 28610835 |
| ica-status of clinical staphylococcus epidermidis strains affects adhesion and aggregation: a thermodynamic analysis. | staphylococcus epidermidis is a major nosocomial pathogen associated with infections of indwelling medical devices. one important virulence factor of these organisms is their ability to adhere to devices and form biofilms. in this study, we evaluated the effect of the ica operon on cell surface hydrophobicity, thermodynamics of adhesion, and biofilm formation for seven s. epidermidis strains. the surface free energy parameters of the bacterial cell surface and the substratum were determined by c ... | 2017 | 28608317 |
| dalbavancin treatment of prosthetic knee infection due to oxacillin-resistant staphylococcus epidermidis. | | 2017 | 28600171 |
| delivery of cyclodextrin polymers to bacterial biofilms - an exploratory study using rhodamine labelled cyclodextrins and multiphoton microscopy. | cyclodextrin (cd) polymers are interesting nanoparticulate systems for pharmaceutical delivery; however, knowledge regarding their applications towards delivery into complex microbial biofilm structures is so far limited. the challenge is to demonstrate penetration and transport through the biofilm and its exopolysaccharide matrix. the ideal functionalization for penetration into mature biofilms is unexplored. in this paper, we present a novel set of rhodamine labelled βcd-polymers, with differe ... | 2017 | 28596141 |
| virulence factors associated with coagulase negative staphylococci isolated from human infections. | infections caused by commensal organisms by changing to infectious life style generate much challenge to the current treatment strategies. coagulase negative staphylococci (cons) are one of them, with their coexisting biofilm forming and multiple antibiotic resistance properties form important agents of nosocomial infection. to evaluate species distribution, biofilm formation, and antibiogram, cons isolates from various clinical samples were isolated. the presence of biofilm and associated genes ... | 2017 | 28593524 |
| chronic sinusitis in a patient with selective igg4 subclass deficiency controlled with enriched immunoglobulins. | a 71-year-old female patient with no major history of infection had presented with recurrent chronic purulent sinusitis over the past 3 years. these recurrent infections started in 2000 with otolaryngologists' support before diagnosis of igg4 deficiency be asked. the patient was treated with increasingly extensive courses of antibiotics and underwent several maxillary and sphenoidal sinus washouts. she continued to present with purulent nasal discharge containing staphylococcus epidermidis. the ... | 2017 | 28588812 |
| randomised controlled trial of topical antibacterial manuka (leptospermum species) honey for evaporative dry eye due to meibomian gland dysfunction. | the aim was to evaluate the efficacy of standardised manuka (leptospermum species) antibacterial honey as adjunctive twice daily treatment to conventional therapy (warm compresses, lid massage and preservative-free lubricant), in participants with evaporative dry eye due to moderate to advanced meibomian gland dysfunction. | 2017 | 28585260 |
| complete genome sequence of staphylococcus epidermidis 1457. | staphylococcus epidermidis 1457 is a frequently utilized strain that is amenable to genetic manipulation and has been widely used for biofilm-related research. we report here the whole-genome sequence of this strain, which encodes 2,277 protein-coding genes and 81 rnas within its 2.4-mb genome and plasmid. | 2017 | 28572323 |
| prevalence, clonality, and pathogenicity of staphylococcus epidermidis isolates in newborn feces. | coagulase-negative staphylococci (cons) are the most prevalent pathogens causing late-onset sepsis in neonates. the question is whether neonates acquire endemic hospital-adapted clones or incidentally occurring cons strains after birth during their hospital stay. therefore, a prospective study was performed on the prevalence of cons in the stool of babies (born vaginally or by cesarean section) during their first days of life. their clonal relatedness and potential to induce invasive disease wer ... | 2017 | 28560472 |
| analysis of early mesothelial cell responses to staphylococcus epidermidis isolated from patients with peritoneal dialysis-associated peritonitis. | the major complication of peritoneal dialysis (pd) is the development of peritonitis, an infection within the abdominal cavity, primarily caused by bacteria. pd peritonitis is associated with significant morbidity, mortality and health care costs. staphylococcus epidermidis is the most frequently isolated cause of pd-associated peritonitis. mesothelial cells are integral to the host response to peritonitis, and subsequent clinical outcomes, yet the effects of infection on mesothelial cells are n ... | 2017 | 28542390 |
| elaboration of antibacterial plastic surfaces by a combination of antiadhesive and biocidal coatings of natural products. | antibacterial polyolefins surfaces, combining biocidal and antiadhesive properties, were elaborated by a covalent grafting of antimicrobial peptides (amps), able to kill adherent bacteria, on a pre-immobilized hyaluronic acid (ha) layer, able to repel the micro-organisms. different ha activation rate for its immobilization, were used to change ha layer morphology and number of residual free carboxylic acid functions for amps grafting. based on adhesion tests on staphylococcus epidermidis and mic ... | 2017 | 28531875 |
| antibacterial effect of hydrogen peroxide-titanium dioxide suspensions in the decontamination of rough titanium surfaces. | the chemical decontamination of infected dental implants is essential for the successful treatment of peri-implantitis. the aim of this study was to assess the antibacterial effect of a hydrogen peroxide-titanium dioxide (h2o2-tio2) suspension against staphylococcus epidermidis biofilms. titanium (ti) coins were inoculated with a bioluminescent s. epidermidis strain for 8 h and subsequently exposed to h2o2 with and without tio2 nanoparticles or chlorhexidine (chx). bacterial regrowth, bacterial ... | 2017 | 28524724 |
| draft genome sequence of staphylococcus epidermidis clinical strain goi1153754-03-14 isolated from an infected knee prosthesis. | we announce the draft genome sequence of staphylococcus epidermidis clinical strain goi1153754-03-14, isolated from an infected orthopedic prosthesis. the reported genomic sequence will provide valuable information concerning the mechanisms of the biofilm formation on metallic implants. | 2017 | 28522724 |
| high proportions of staphylococcus epidermidis in dental caries harbor multiple classes of antibiotics resistance, significantly increase inflammatory interleukins in dental pulps. | staphylococcus epidermidis is one of most prevalent in dental caries or dental pulp which has the capability of horizontal genetic transfer between different bacterial species in the oropharynx, suggesting that it may evolve with the dissemination of resistant determinants, this study was performed to molecularly characterize and differentiate s. epidermidis isolated from dental caries and healthy individual. also, two important cytokines in inflammation were assayed caused due to s. epidermidis ... | 2017 | 28506885 |
| evaluation of biofilm-forming ability of bacterial strains isolated from the roof of an old house. | the bacterial diversity associated with biofilm-forming ability was studied. eighteen bacterial strains were isolated from a microbial film collected from the roof of an old house located in sfax, tunisia. the purity of these microorganisms was confirmed by microscopic observation after repeated streaking on a tryptic soy agar medium. biofilm formation was estimated using preliminary tests including a motility test, microbial adhesion to solvents (mats), and the congo red agar method (cra). sinc ... | 2017 | 28502956 |
| identification of genes controlled by the essential yycfg two-component system reveals a role for biofilm modulation in staphylococcus epidermidis. | biofilms play a crucial role in the pathogenicity of staphylococcus epidermidis, while little is known about whether the essential yycfg two-component signal transduction system (tcs) is involved in biofilm formation. we used antisense rna (asrna) to silence the yycfg tcs in order to study its regulatory functions in s. epidermidis. strain 1457 expressing asrna yycf exhibited a significant delay (~4-5 h) in entry to log phase, which was partially complemented by overexpressing ssaa. the expressi ... | 2017 | 28491057 |
| fdg pet/ct in aortic valve bioprosthesis infection. | a 62-year-old man presented with persistent fever, weakness, and retrosternal pain 3 years after aortic valve bioprosthesis (avr). his white blood cell count was 11,000/μl and c-reactive protein was 13.6 mg/dl. consecutive blood cultures isolated staphylococcus epidermidis and capitis. transesophageal echocardiography demonstrated small aortic valve prosthesis vegetation. f-fdg pet/ct revealed prominent avr activity, suvmax = 12.2. he was treated with daptomycin, meropenem, and gentamicin for 1 ... | 2017 | 28489611 |
| imaging of intracellular behavior of polymeric nanoparticles in staphylococcus epidermidis biofilms by slit-scanning confocal raman microscopy and scanning electron microscopy with energy-dispersive x-ray spectroscopy. | in drug delivery systems employing polymeric nanoparticles, accurate delivery of drugs to target sites such as bacterial cells, cell tissues, and organelles is essential. in particular, when designing drug delivery systems for the treatment of the biofilm infections, evaluation of the interaction between polymeric nanoparticles and biofilm or bacterial cells using a simple technique is of significant importance. here we develop two types of novel techniques for the biological imaging of the intr ... | 2017 | 28482470 |
| effect of subinhibitory concentrations of tigecycline and ciprofloxacin on the expression of biofilm-associated genes and biofilm structure of staphylococcus epidermidis. | staphylococcus epidermidis is a leading cause of foreign body-associated infections. this is related to the bacterium's ability to form biofilms on synthetic materials. bacteria within a biofilm may be exposed to subinhibitory concentrations (sub-mics) of antibiotics because of an agent's limited penetration into the biofilm core. here, we investigated the effect of sub-mics of tigecycline and ciprofloxacin on the expression of biofilm-associated genes, i.e. icaa, alte and sigb, and the biofilm ... | 2017 | 28481197 |
| comparison of bacterial attachment to platelet bags with and without preconditioning with plasma. | canadian blood services produces apheresis and buffy coat pooled platelet concentrates (pcs) stored in bags produced by two different manufacturers (a and b, respectively), both made of polyvinyl chloride-butyryl trihexyl citrate. this study was aimed at comparing staphylococcus epidermidis adhesion to the inner surface of both bag types in the presence or absence of plasma factors. | 2017 | 28466544 |
| correction for rabinovitch and stewart, "removal and inactivation of staphylococcus epidermidis biofilms by electrolysis". | | 2017 | 28461323 |
| intrathecal baclofen pump infection treated by adjunct intrareservoir teicoplanin instillation. | the delivery of intrathecal baclofen via pumps is gaining increasing use in the management of intractable spasticity. one of the rare but devastating complications of this method is infection. in the majority of cases, removal of the device is required, despite appropriate intravenous antibiotic therapy. we report a case that highlights the use of intrareservoir teicoplanin to achieve sterilization of the infected pump system in a patient in whom removal of the pump was not an easy option. | 2017 | 28458952 |
| growth inhibition of an opportunistic yeast pathogen trichosporon asahii by staphylococcus epidermidis. | in the co-culture of staphylococcus epidermidis and trichosporon asahii, a fungal pathogen, it was observed that live s. epidermidis inhibited the growth of t. asahii. soluble active anti-t. asahii substances were speculated to be produced by s. epidermidis in culture medium. using (1)h- and (13)c-nmr spectra and electron ionization-high resolution mass spectrometry (hr-negative-fab-ms), we separated the active molecule and identified it as lactic acid. commercially available l-lactic acid and d ... | 2017 | 28458355 |
| left ventricular assist pump pocket infection: conservative treatment strategy for destination therapy candidates. | heart failure is a major cause of mortality and morbidity, particularly among patients with advanced disease and no access to cardiac transplantation. lvad implantation is not only a bridge-to-transplantation option for patients awaiting a heart donor, but is often used as bridge-to-destination therapy in patients unsuited for transplantation for various reasons. lvad infection is considered the second-most common cause of death in patients who survive the initial 6 months on lvad support. few r ... | 2017 | 28430300 |
| fibrin sheath-associated endovascular infection of the heart: the trojan horse of indwelling central venous catheters. | chronic indwelling central venous catheters can result in formation of fibrin sheaths increasing risk of occlusion, thrombosis and infection. endovascular infection of right-sided heart structures induced by such sheaths is very rare. a 48-year-old woman with end-stage renal disease initially treated for diabetic ketoacidosis developed persistent staphylococcus epidermidis bacteraemia without an identifiable source. although transthoracic echocardiography was unremarkable, transoesophageal echoc ... | 2017 | 28420633 |
| disclosing agents for the intraoperative identification of biofilms on orthopedic implants. | disclosing agents are dyes used in dentistry to colorize plaque (biofilm) and may offer a means for intraoperative detection of biofilms on orthopedic implants. methylene blue (mb) stains biofilm and is safely used in orthopedic applications. injection of mb into acutely infected prosthetic knees before debridement may enable visualization of biofilm, which could influence treatment decisions. the aims of this study were to determine if mb could be used to visualize biofilm on total knee arthrop ... | 2017 | 28420544 |
| coagulase-negative staphylococci in human milk from mothers of preterm compared with term neonates. | human milk is the preferred nutrition for neonates and a source of bacteria. research aim: the authors aimed to characterize the molecular epidemiology and genetic content of staphylococci in the human milk of mothers of preterm and term neonates. | 2017 | 28418807 |
| modulation of staphylococcus epidermidis (rp62a) extracellular polymeric layer by marine cyclic dipeptide-cyclo(l-leucyl-l-prolyl) thwarts biofilm formation. | staphylococcus epidermidis is the most frequent cause of biofilm mediated implant-associated infections. extracellular polymeric substance (eps) is a key component of most biofilms and in pathogens it specifically protects the entrenched-bacterial cells from antibiotics and hosts immune response, and thereby makes the infection ineradicable. recently, the prominence of cyclic dipeptides in interfering with biofilms and the associated virulence factors of pathogens has offered an alternative to e ... | 2017 | 28414038 |
| prevalence of bacterial contamination in platelet concentrates at the national center of blood transfusion (mexico). | most common bacterial sepsis associated with transfusion is caused by contaminated platelet concentrates (pc). the screening of pc to detect bacterial contamination is obligatory in mexico, and it is carried out in quality control programs. in mexico, the identification and molecular characterization of bacterial contaminants to detect contamination sources have not been implemented due to high costs; however, it is an actual current need. | 2017 | 28412024 |
| optimizing the antisepsis protocol: effectiveness of 3 povidone-iodine 1.0% applications versus a single application of povidone-iodine 5.0. | to determine the minimum effective concentration of povidone-iodine that reduces the bacterial load by 3-log10, the u.s. food and drug administration requirement for antiseptic agents, and to study alternative dosing schedules of povidone-iodine to optimize its bactericidal effect. | 2017 | 28410725 |
| comparison of biofilm formation capacities of two clinical isolates of staphylococcus epidermidis with and without icaa and icad genes on intraocular lenses. | to compare biofilm formations of two staphylococcus epidermidis (s. epidermidis) isolates with known biofilm formation capacities on four different intraocular lenses (iol) that have not been studied before. | 2017 | 28405479 |
| direct-contact low-frequency ultrasound clearance of biofilm from metallic implant materials. | introduction: biofilms are recognized as a significant deterrent to wound healing and to the management of exposed or infected surgical implants. biofilms can be disrupted by a variety of enzymatic and mechanical interventions. this experiment was designed to determine whether direct-contact low-frequency ultrasound has the ability to clear biofilms and what then happens to the released bacteria. methods:staphylococcus epidermidis biofilm was grown on the surfaces of metallic discs composed of t ... | 2017 | 28405263 |
| characterization of staphylococcal cassette chromosome mec (sccmec) in methicillin-resistant staphylococcus epidermidis strains isolated from biomaterial-associated infections and their antibiotic resistance patterns. | this work aims to provide an insight into staphylococcal cassette chromosome mec elements and antibiotic resistance in clinical isolates of staphylococcus epidermidis. the dominating type was sccmec – iv. fifteen isolates were assigned to sccmec type iii, two isolates to sccmec type ii. most isolates were resistant to at least three of the non-β-lactam antibiotics tested. none of the strains exhibited resistance to new generation antibiotics, such as daptomycin and linezolid. also, none of these ... | 2016 | 28520329 |