| staphylococcus epidermidis bacteremia induces brain injury in neonatal mice via toll-like receptor 2-dependent and -independent pathways. | staphylococcus epidermidis causes late-onset sepsis in preterm infants. staphylococcus epidermidis activates host responses in part via toll-like receptor 2 (tlr2). epidemiologic studies link bacteremia and neonatal brain injury, but direct evidence is lacking. | 2015 | 25883383 |
| augmented renal vancomycin clearance in cancer patients: a case report and review of the literature. | bacterial infections are a major cause of morbidity and mortality in cancer patients. particularly diagnostic and therapeutic procedures (e.g. central venous catheters, paracentesis) increase the risk of infections in this immunocompromised patient population. in the past, antibiotic therapy was empirically initiated in these patients, guided by treatment regimens designed for patients without malignancy; however, the hyperdynamic circulation in systemic inflammatory response syndrome, as well a ... | 2015 | 25877942 |
| effect of amino acid substitution in the staphylococcal peptides warnericin rk and psmα on their anti-legionella and hemolytic activities. | warnericin rk from staphylococcus warneri and psmα from staphylococcus epidermidis are anti-legionella peptides which were differently classified in a previous study according to their mode of action. indeed, warnericin rk is highly hemolytic with a bactericidal mode of action, whereas psmα is poorly hemolytic with a bacteriostatic mode of action toward l. pneumophila. in order to find anti-legionella peptides which are not hemolytic, a collection of peptides varying in sequence from warnericin ... | 2015 | 25869678 |
| successful use of daptomycin in a preterm neonate with persistent methicillin-resistant staphylococcus epidermidis bacteremia. | there is limited information regarding the use of daptomycin in the neonatal population, and dosage adjustments for neonates with renal dysfunction. we report on the successful use of daptomycin in a 1-month-old, former 24-week gestation neonate with persistent methicillin-resistant staphylococcus epidermidis (mrse) bacteremia and impaired renal function. we also review the available literature supporting daptomycin use in the neonatal period. daptomycin peak and trough serum levels were obtaine ... | 2017 | 25859172 |
| atypical presentation of acute-onset endophthalmitis after uncomplicated pars plana vitrectomy. | a 70-year-old woman presented with signs of acute-onset endophthalmitis within 24 hours of surgery, with retinal hemorrhages and vascular changes in the absence of pain or significant inflammation. she had undergone uncomplicated 25-gauge pars plana vitrectomy for epiretinal membrane with presenting visual acuity of 20/40- and significant distortion. vitreous culture revealed staphylococcus epidermidis. final visual acuity was no light perception with persistent pain, and the patient ultimately ... | 2015 | 25856829 |
| the inhibition the tet(k) efflux pump of tetracycline resistant staphylococcus epidermidis by essential oils from three salvia species. | the inhibition of efflux pumps is an attractive and powerful response to the emergence of bacteria resistant to antibiotics. essential oils (eos) from salvia fruticosa, salvia officinalis and salvia sclarea reduce the minimal inhibition concentration of tetracycline, decrease efflux of antibiotic and decrease the expression of tet(k) gene in tetracycline resistant clinical isolates of staphylococcus epidermidis. in all the cases s. fruticosa was the best one. by using checkerboard and time-killi ... | 2015 | 25846244 |
| tio2 nanotube composite layers as delivery system for zno and ag nanoparticles - an unexpected overdose effect decreasing their antibacterial efficacy. | enhancement of biocompatibility and antibacterial properties of implant materials is potentially beneficial for their practical value. therefore, the use of metallic and metallic oxide nanoparticles as antimicrobial coatings components which induce minimized antibacterial resistance receives currently particular attention. in this work, tio2 nanotubes layers loaded with zno and ag nanoparticles were designed for biomedical coatings and delivery systems and evaluated for antimicrobial activity. t ... | 2015 | 25842121 |
| analyzing the antibacterial effects of food ingredients: model experiments with allicin and garlic extracts on biofilm formation and viability of staphylococcus epidermidis. | to demonstrate different effects of garlic extracts and their main antibiotic substance allicin, as a template for investigations on the antibacterial activity of food ingredients. staphylococcus epidermidis atcc 12228 and the isogenic biofilm-forming strain atcc 35984 were used to compare the activity of allicin against planktonic bacteria and bacterial biofilms. the minimal inhibitory concentration (mic) and the minimum biofilm inhibitory concentration (mbic) for pure allicin were identical an ... | 2015 | 25838894 |
| a commensal strain of staphylococcus epidermidis overexpresses membrane proteins associated with pathogenesis when grown in biofilms. | staphylococcus epidermidis has emerged as one of the major nosocomial pathogens associated with infections of implanted medical devices. the most important factor in the pathogenesis of these infections is the formation of bacterial biofilms. bacteria grown in biofilms are more resistant to antibiotics and to the immune defence system than planktonic bacteria. in these infections, the antimicrobial therapy usually fails and the removal of the biofilm-coated implanted device is the only effective ... | 2015 | 25837994 |
| staphylococcus epidermidis affinity for fibrinogen-coated surfaces correlates with the abundance of the sdrg adhesin on the cell surface. | staphylococcus epidermidis is a world-leading pathogen in healthcare facilities, mainly causing medical device-associated infections. these nosocomial diseases often result in complications such as bacteremia, fibrosis, or peritonitis. the virulence of s. epidermidis relies on its ability to colonize surfaces and develop thereupon in the form of biofilms. bacterial adherence on biomaterials, usually covered with plasma proteins after implantation, is a critical step leading to biofilm infections ... | 2015 | 25821995 |
| evaluation of various staphylococcal cassette chromosome mec (sccmec) types in staphylococcus epidermidis invasive strains from hospitalised patients in iran. | staphylococcus epidermidis is known to be a major cause of nosocomial infections particularly in catheter-associated bacteraemia, prosthetic valve endocarditis (pve) and immunocompromised patients in different health care units. the emergence of multidrug-resistant strains, especially to β-lactam antibiotics such as methicillin, has increased the mortality due to s. epidermidis. a kind of low affinity penicillin-binding protein (pbp2α), which is encoded by the meca gene that is located in the st ... | 2015 | 25819046 |
| interconverting flavonostilbenes with antibacterial activity from sophora alopecuroides. | five flavonostilbenes (alopecurones h, i, j, k and l) and five known ones were isolated from roots of sophora alopecuroides, in addition to ten other phenolic compounds. a non-enzymatic interconversion of the lavandulyl-substituted flavonostilbenes was observed among alopecurones a, h, i, and k through a wessely-moser rearrangement reaction; this was proven by 1d and 2d nmr, hplc-cd-pda and hrms analyses. bioassay results suggested that flavonostilbenes exhibit significant antibacterial and anti ... | 2015 | 25813880 |
| infected prostheses after lower-extremity bone tumor resection: clinical outcomes of 100 patients. | infection of megaprostheses after bone tumor resection is a major concern; management is challenging. this study evaluated the survivability from such infections, the microbial isolates, treatment tactics, and outcome of megaprosthesis reconstructions. | 2015 | 25812073 |
| [regulatory effect of two component signal transduction system saers on the survival of staphylococcus epidermidis]. | to investigate the survival ability of staphylococcus epidermidis (s. epidermidis) wildtype (wt), saers mutant (sae) and saers complementary (saec) strains under different concentrations of glucose. | 2015 | 25807791 |
| biofilm-forming staphylococcus epidermidis expressing vancomycin resistance early after adhesion to a metal surface. | we investigated biofilm formation and time of vancomycin (vcm) resistance expression after adhesion to a metal surface in staphylococcus epidermidis. biofilm-forming staphylococcus epidermidis with a vcm mic of 1 μg/ml was used. the bacteria were made to adhere to a stainless steel washer and treated with vcm at different times and concentrations. vcm was administered 0, 2, 4, and 8 hours after adhesion. the amount of biofilm formed was evaluated based on the biofilm coverage rates (bcrs) before ... | 2015 | 25802873 |
| possible correlation between bile salt hydrolysis and ahl deamidation: staphylococcus epidermidis rm1, a potent quorum quencher and bile salt hydrolase producer. | the aim of the present work was to isolate a bile salt hydrolase (bsh) producer from fermented soy curd and explore the ability of the bsh produced to cleave bacterial quorum sensing signals. bacterial isolates with possible ability to deconjugate bile salts were enriched and isolated on de man, rogosa and sharpe (mrs) medium containing 0.2% bile salts. bsh-producing positive isolate with orange-pink-pigmented colonies was isolated and was identified as a strain of staphylococcus epidermidis usi ... | 2015 | 25800527 |
| an 18 kda scaffold protein is critical for staphylococcus epidermidis biofilm formation. | virulence of the nosocomial pathogen staphylococcus epidermidis is crucially linked to formation of adherent biofilms on artificial surfaces. biofilm assembly is significantly fostered by production of a bacteria derived extracellular matrix. however, the matrix composition, spatial organization, and relevance of specific molecular interactions for integration of bacterial cells into the multilayered biofilm community are not fully understood. here we report on the function of novel 18 kda small ... | 2015 | 25799153 |
| immunoreactive pattern of staphylococcus epidermidis biofilm against human whole saliva. | saliva is essential to interact with microorganisms in the oral cavity. therefore, the interest in saliva antimicrobial properties is on the rise. here, we used an immunoproteomic approach, based on protein separation of staphylococcus epidermidis biofilms by 2de, followed by western-blotting, to compare human serum and saliva reactivity profile. a total of 17 proteins were identified by maldi-tof/tof. serum and saliva presented a distinct pattern of immunoreactive proteins. our results suggest ... | 2015 | 25782040 |
| observation of antibacterial effect of biodegradable polymeric nanoparticles on staphylococcus epidermidis biofilm using fe-sem with an ionic liquid. | we successfully visualized the antibacterial behavior of biodegradable polymeric nanoparticles (nps) on a biofilm formed by staphylococcus epidermidis using field emission scanning electron microscopy (fe-sem). a hydrophilic ionic liquid, 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim][bf4]), was applied for observation using fe-sem. the differences in adherence and penetration behavior of three types of nps were revealed using this method and confocal laser scanning microscopy. biodegrada ... | 2015 | 25757698 |
| an immunoproteomic approach for characterization of dormancy within staphylococcus epidermidis biofilms. | virulence of staphylococcus epidermidis is mainly attributed to surface colonization and biofilm formation in indwelling medical devices. physiological heterogeneity of biofilms may influence host immune response and sensitivity to antibiotics. dormant cells, among others, contribute to biofilm heterogeneity. the aim of this study was to identify immunogenic proteins of s. epidermidis biofilms associated with dormancy mechanism, by using two-dimensional electrophoresis (2-de) immunoblotting and ... | 2015 | 25749707 |
| cadmium modulates biofilm formation by staphylococcus epidermidis. | the aim of the study was to evaluate the effect of cadmium exposure on staphylococcus epidermidis (atcc 35984) biofilm formation. bacteria were cultured in the absence or presence of different concentrations (0-50 µm) of cadmium. biofilm formation and bacterial viability were assessed. quantitative real time-pcr (qrt-pcr) was used to determine the mrna expression of molecular markers of s. epidermidis biofilm formation and dispersion. s. epidermidis biofilm formation was stimulated (p<0.001) by ... | 2015 | 25749322 |
| the role of plasma, albumin, and fibronectin in staphylococcus epidermidis adhesion to polystyrene surface. | the influence of soluble and immobilized plasma, albumin, and fibronectin (fn) on the adhesion of three staphylococcus epidermidis strains to polystyrene was investigated. both soluble and immobilized plasma and albumin cause to 7-fold reduction of the amounts of adhered cells, regardless of the strain used. the soluble fn exhibited the adhesion for one strain and did not affect the bacterial sorption for remaining strains, whereas on fn-coated polystyrene two of the three strains showed about 1 ... | 2015 | 25744155 |
| structural basis of staphylococcus epidermidis biofilm formation: mechanisms and molecular interactions. | staphylococcus epidermidis is a usually harmless commensal bacterium highly abundant on the human skin. under defined predisposing conditions, most importantly implantation of a medical device, s. epidermidis, however, can switch from a colonizing to an invasive life style. the emergence of s. epidermidis as an opportunistic pathogen is closely linked to the biofilm forming capability of the species. during the past decades, tremendous advance regarding our understanding of molecular mechanisms ... | 2015 | 25741476 |
| linezolid resistance in clinical isolates of staphylococcus epidermidis from german hospitals and characterization of two cfr-carrying plasmids. | this study was a detailed investigation of staphylococcus epidermidis clinical isolates exhibiting linezolid resistance. | 2015 | 25740949 |
| identification of individual biofilm-forming bacterial cells using raman tweezers. | a method for in vitro identification of individual bacterial cells is presented. the method is based on a combination of optical tweezers for spatial trapping of individual bacterial cells and raman microspectroscopy for acquisition of spectral “raman fingerprints” obtained from the trapped cell. here, raman spectra were taken from the biofilm-forming cells without the influence of an extracellular matrix and were compared with biofilm-negative cells. results of principal component analyses of r ... | 2015 | 25734616 |
| autophagy and mitochondrial remodelling in mouse mesenchymal stromal cells challenged with staphylococcus epidermidis. | the bone marrow stroma constitutes the marrow-blood barrier, which sustains immunochemical homoeostasis and protection of the haematopoietic tissue in sequelae of systemic bacterial infections. under these conditions, the bone marrow stromal cells affected by circulating bacterial pathogens shall elicit the adaptive stress-response mechanisms to maintain integrity of the barrier. the objective of this communication was to demonstrate (i) that in vitro challenge of mesenchymal stromal cells, i.e. ... | 2015 | 25721260 |
| continuous aerosol size separator using inertial microfluidics and its application to airborne bacteria and viruses. | a microchannel-based aerosol size separator that separates submicron aerosols according to particle inertial differences and dean vortices in the airflow was developed for use in low-cost, portable, real-time aerosol collectors, detectors, concentrators and other such devices. the microfluidic inertial separator was furthermore applied to simultaneously separate airborne microorganisms by size, such as airborne viruses and bacteria from larger aerosols and viral particles from bacterial cells. t ... | 2015 | 25714231 |
| biofilm-like aggregation of staphylococcus epidermidis in synovial fluid. | | 2015 | 25712965 |
| wide dissemination of linezolid-resistant staphylococcus epidermidis in greece is associated with a linezolid-dependent st22 clone. | dependence on linezolid was recently described as significant growth acceleration of linezolid-resistant staphylococcus epidermidis (lrse) isolates upon linezolid exposure. we investigated the possible contribution of linezolid dependence to lrse dissemination in greece. | 2015 | 25712317 |
| lead-dependent infective endocarditis: the role of factors predisposing to its development in an analysis of 414 clinical cases. | lead-dependent infective endocarditis (ldie) is a serious and insidious infective disease spreading along the leads to valve leaflets and endocardial surface. ldie is still a lesser known disease with unclear risk factors, most often evaluated jointly for all infectious complications. | 2015 | 25683205 |
| endophthalmitis after resident-performed intravitreal bevacizumab injection. | to evaluate the rate of acute endophthalmitis after resident-performed intravitreal bevacizumab (ivb) injections and to compare the results with those performed by attending retina surgeons. | 2015 | 25677280 |
| antimicrobial resistance and population structure of staphylococcus epidermidis recovered from pig farms in belgium. | pigs are known to harbour a variety of staphylococcal bacteria, including staphylococcus epidermidis, in the upper respiratory tract. the aim of the present study was to determine the prevalence, genetic diversity, virulence and antimicrobial resistance of s. epidermidis in healthy pigs, as well as to identify the potential role of pigs as a reservoir of zoonotic infection. the overall prevalence of s. epidermidis carriage was 28%, with approximately half of the pigs tested (13.5%) carrying meth ... | 2015 | 25676880 |
| proteomic profile of dormancy within staphylococcus epidermidis biofilms using itraq and label-free strategies. | staphylococcus epidermidis is an important nosocomial bacterium among carriers of indwelling medical devices, since it has a strong ability to form biofilms. the presence of dormant bacteria within a biofilm is one of the factors that contribute to biofilm antibiotic tolerance and immune evasion. here, we provide a detailed characterization of the quantitative proteomic profile of s. epidermidis biofilms with different proportions of dormant bacteria. a total of 427 and 409 proteins were identif ... | 2015 | 25672847 |
| self-defensive antibacterial layer-by-layer hydrogel coatings with ph-triggered hydrophobicity. | we report on negatively charged layer-by-layer (lbl) hydrogel films, which turn hydrophobic and bactericidal in response to bacteria-induced acidification of the medium. single-component hydrogel thin films, abbreviated as paaalbls, consisting of chemically crosslinked poly(2-alkylacrylic acids) (paaas) with varying hydrophobicity [polymethacrylic acid (pmaa), poly(2-ethylacrylic acid) (peaa), poly(2-n-propylacrylic acid) (ppaa) or poly(2-n-butylacrylic acid) (pbaa)]. with increasing polyacid hy ... | 2015 | 25662496 |
| nanostructured architectures by assembling polysaccharide-coated bsa nanoparticles for biomedical application. | nanostructured architectures are produced on ti surfaces by layer-by layer (lbl) self-assembling of polysaccharide-coated bsa nanoparticles (bnps), which created cellular microenvironments mimicking natural extracellular matrix. the bmp-2 encapsulated bnps are prepared by a desolvation method, and are further coated by chitosan (chi) coatings to obtain positively charged nps (cbnps). vancomycin (van) encapsulated cbnps are obtained by the same method and subsequently coated by oxidized alginate ... | 2015 | 25656491 |
| incidence, management and outcomes of the first cfr-mediated linezolid-resistant staphylococcus epidermidis outbreak in a tertiary referral centre in the republic of ireland. | to report the first irish outbreak of cfr-mediated linezolid-resistant staphylococcus epidermidis. | 2015 | 25648941 |
| enantiomerically pure 3-aryl- and 3-hetaryl-2-hydroxypropanoic acids by chemoenzymatic reduction of 2-oxo acids. | phenyllactic acids are found in numerous natural products as well as in active substances used in medicine or plant protection. enantiomerically pure phenyllactic acids are available by transition-metal-catalyzed hydrogenations or chemoenzymatic reductions of the corresponding 3-aryl-2-oxopropanoic acids. we show here that d-lactate dehydrogenase from staphylococcus epidermidis reduces a broad spectrum of 2-oxo acids, which are difficult substrates for transition-metal-catalyzed reactions, with ... | 2015 | 25647633 |
| autoinducer-2 increases biofilm formation via an ica- and bhp-dependent manner in staphylococcus epidermidis rp62a. | staphylococcus epidermidis has become the most common cause of nosocomial bacteraemia and the principal organism responsible for indwelling medical device -associated infections. its pathogenicity is mainly due to its ability to form biofilms on the implanted medical devices. biofilm formation is a quorum-sensing (qs)-dependent process controlled by autoinducers, which are signalling molecules. here, we investigated the function of the autoinducer-2 (ai-2) qs system, especially the influence of ... | 2015 | 25637952 |
| inefficacy of vancomycin and teicoplanin in eradicating and killing staphylococcus epidermidis biofilms in vitro. | biofilm-associated bacteria display a decreased susceptibility towards antibiotics. routine assessment of antibiotic susceptibility of planktonic bacteria therefore offers an insufficient prediction of the biofilm response. in this study, in vitro biofilms of eight clinical staphylococcus epidermidis strains were subjected to treatment with vancomycin, teicoplanin, oxacillin, rifampicin and gentamicin. in addition, the biofilms were subjected to combinations of an antibiotic with rifampicin. the ... | 2015 | 25614358 |
| effects of temperature on the morphological, polymeric, and mechanical properties of staphylococcus epidermidis bacterial biofilms. | changes in temperature were found to affect the morphology, cell viability, and mechanical properties of staphylococcus epidermidis bacterial biofilms. s. epidermidis biofilms are commonly associated with hospital-acquired medical device infections. we observed the effect of heat treatment on three physical properties of the biofilms: the bacterial cell morphology and viability, the polymeric properties of the extracellular polymeric substance (eps), and the rheological properties of the bulk bi ... | 2015 | 25602470 |
| linezolid-resistant staphylococcal bacteraemia: a multicentre case-case-control study in italy. | the aim of this multicentre study was to analyse the characteristics of patients with bloodstream infections due to staphylococcal strains resistant to linezolid. this was a retrospective case-case-control study of patients hospitalised in three large teaching hospitals in italy. a linezolid-resistant (lin-r) staphylococcus spp. group and a linezolid-susceptible (lin-s) staphylococcus spp. group were compared with control patients to determine the clinical features and factors associated with is ... | 2015 | 25600893 |
| dual effects of β-cyclodextrin-stabilised silver nanoparticles: enhanced biofilm inhibition and reduced cytotoxicity. | the composition and mode of synthesis of nanoparticles (nps) can affect interaction with bacterial and human cells differently. the present work describes the ability of β-cyclodextrin (β-cd) capped silver nanoparticles (agnps) to inhibit biofilm growth and reduce cytotoxicity. biofilm formation of staphylococcus epidermidis csf 41498 was quantified by a crystal violet assay in the presence of native and capped agnps (ag-10cd and ag-20cd), and the morphology of the biofilm was observed by scanni ... | 2015 | 25596861 |
| potential spread of multidrug-resistant coagulase-negative staphylococci through healthcare waste. | healthcare waste (hcw) might potentially harbor infective viable microorganisms in sanitary landfills. we investigated the antimicrobial susceptibility patterns and the occurrence of the meca gene in coagulase-negative staphylococcus strains (cons) recovered from the leachate of the hcw in an untreated sanitary landfill. | 2015 | 25596568 |
| staphylococcus epidermidis in urine is not always benign: a case report of pyelonephritis in a child. | staphylococcus epidermidis in urine cultures from previously healthy children is usually considered to be a contaminant. the goal of this study was to identify cases in which s. epidermidis was an infectious cause of urinary tract infection (uti) in children. | 2015 | 25567836 |
| investigation of linezolid resistance in staphylococcus epidermidis: first reported linezolid resistant coagulase negative staphylococcus in turkey. | | 2014 | 25552175 |
| media-fill simulation tests in manual and robotic aseptic preparation of injection solutions in syringes. | the purpose of this study was to evaluate the contamination rate of media-fill products either prepared automated with a robotic system (apotecachemo™) or prepared manually at cytotoxic workbenches in the same cleanroom environment and by experienced operators. media fills were completed by microbiological environmental control in the critical zones and used to validate the cleaning and disinfection procedures of the robotic system. | 2016 | 25549919 |
| [antibiotic resistance of coagulase-negative staphylococci isolated at hospitals of st. petersburg and moscow]. | antibiotic susceptibility of 119 coagulase-negative staphylococci isolated at hospitals of st. petersburg and moscow was investigated and estimated at the local laboratories as oxacillin resistant. the following species were identified: staphylococcus epidermidis, s. haemolyticus, s. hominis, s.capitis, s. simulans, s. pettenkoferi, s. lentus, s. carnosus and s. warneri. the oxacillin resistance was confirmed in 79.8% of the isolates. the frequency of the associated resistance to non-beta-lactam ... | 2015 | 27141635 |
| evaluation of nosocomial infection in patients at hematology-oncology ward of dr. sheikh children's hospital. | infections in critical care unit are high, and they are serious hospital problems. infections acquired during the hospital stay are generally called nosocomial infections, initially known as infections arising after 48 h of hospital admission. the mostfrequent nosocomial infections (urinary, respiratory, gastroenteritis and blood stream infection) were common in patients at hospital.the aim was to study, the current status of nosocomial infection, rate of infection among hospitalized children at ... | 2015 | 26985350 |
| electron microscopy of staphylococcus epidermidis fibril and biofilm formation using image-enhancing ionic liquid. | we established an optimized biofilm observation method using a hydrophilic ionic liquid (il), 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim][bf4]). in the present study, a biofilm was formed by staphylococcus epidermidis. using field emission (fe) scanning electron microscopy (sem) and transmission electron microscopy (tem), the colonization of assemblages formed by microbial cells was observed as a function of the cultivation time. fe-tem analysis revealed that the fibril comprises three ... | 2015 | 25542577 |
| commensal-dendritic-cell interaction specifies a unique protective skin immune signature. | the skin represents the primary interface between the host and the environment. this organ is also home to trillions of microorganisms that play an important role in tissue homeostasis and local immunity. skin microbial communities are highly diverse and can be remodelled over time or in response to environmental challenges. how, in the context of this complexity, individual commensal microorganisms may differentially modulate skin immunity and the consequences of these responses for tissue phys ... | 2015 | 25539086 |
| a novel soft tissue model for biomaterial-associated infection and inflammation - bacteriological, morphological and molecular observations. | infection constitutes a major risk for implant failure, but the reasons why biomaterial sites are more vulnerable than normal tissue are not fully elucidated. in this study, a soft tissue infection model was developed, allowing the analysis of cellular and molecular responses in each of the sub-compartments of the implant-tissue interface (on the implant surface, in the surrounding exudate and in the tissue). smooth and nanostructured titanium disks with or without noble metal chemistry (silver, ... | 2015 | 25522970 |
| efficacy of dressing with absorbent foam versus dressing with gauze in prevention of tracheostomy site infection. | tracheostomy site infection can cause numerous problems. absorbent foam dressing may be able to prevent proliferation of infectious microorganisms by absorbing the tracheostomy stoma exudate. this study aimed to compare the efficacy of absorbent foam with gauze dressing for prevention of tracheostomy site infection. | 2014 | 25506371 |
| impact of staphylococcus epidermidis lysates on middle ear epithelial proinflammatory and mucogenic response. | chronic otitis media with effusion (come) develops after sustained inflammation and is characterized by secretory middle ear epithelial metaplasia and effusion, most frequently mucoid. staphylococcus epidermidis, typically considered a commensal organism, is very frequently recovered in chronic middle ear fluid and in middle ear biofilms. although it has been shown to drive inflammation in sinonasal epithelium, the impact of s. epidermidis on come is markedly understudied. the goal of this study ... | 2015 | 25503091 |
| inhibition of bacterial mevalonate diphosphate decarboxylase by eriochrome compounds. | mevalonate diphosphate decarboxylase (mdd; ec 4.1.1.33) catalyzes the irreversible decarboxylation of mevalonate diphosphate in the mevalonate pathway to form isopentenyl diphosphate, which is a precursor in the biosynthesis of many essential polyisoprenoid natural products, including sterols. in low g/c gram-positive bacteria, which utilize the mevalonate pathway, mdd is required for cell viability and thus is a potential target for development of antibiotic drugs. to identify potential inhibit ... | 2015 | 25499551 |
| activity of bone cement loaded with daptomycin alone or in combination with gentamicin or peg600 against staphylococcus epidermidis biofilms. | daptomycin is a promising candidate for local treatment of bone infection due to its activity against multi-resistant staphylococci. we investigated the activity of antibiotic-loaded pmma against staphylococcus epidermidis biofilms using an ultra-sensitive method bacterial heat detection method (microcalorimetry). pmma cylinders loaded with daptomycin alone or in combination with gentamicin or peg600, vancomycin and gentamicin were incubated with s. epidermidis-rp62a in tryptic soy broth (tsb) f ... | 2015 | 25498330 |
| assessing and reducing sources of gene expression variability in staphylococcus epidermidis biofilms. | gene expression quantification can be a useful tool in studying the properties of bacterial biofilms. unfortunately, techniques such as rna extraction, cdna synthesis, and quantitative pcr (qpcr) can introduce variability into mrna transcript measurements, obscuring biologically relevant results. here we sought to identify the steps that impair accurate gene expression quantification from staphylococcus epidermidis biofilm samples. we devised an experimental setup that could be used to determine ... | 2014 | 25495729 |
| the efficiency of cross-linking methods in eradication of bacteria is influenced by the riboflavin concentration and the irradiation time of ultraviolet light. | to determine bacterial eradication using numerous riboflavin concentrations and different ultraviolet light a (uva) radiant and exposure time in an experimental model. | 2014 | 25493311 |
| role of protein environment and bioactive polymer grafting in the s. epidermidis response to titanium alloy for biomedical applications. | joint implant-related infections, namely by staphylococci, are a worldwide problem, whose consequences are dramatic. various methods are studied to fight against these infections. here, the proposed solution consists in grafting a bioactive polymer on joint implant surfaces in order to allow the control of the interactions with the living system. in this study, sodium styrene sulfonate, bearing sulfonate groups, was grafted on the surface of titanium alloys. scanning electron microscopy, colorim ... | 2014 | 25491817 |
| antibiotic susceptibility and meca frequency in staphylococcus epidermidis, isolated from intensive care unit patients. | coagulase-negative staphylococci (cons), especially staphylococcus epidermidis, are considered as normal flora of human epithelia and also important opportunistic pathogens for nosocomial infections. s. epidermidis can also act as a reservoir for meca, responsible for high-level resistance to methicillin and transferring it to s. aureus. | 2014 | 25485050 |
| sanitary quality, occurrence and identification of staphylococcus sp. in food services. | sanitary conditions are essential for the production of meals and control of the presence of pathogensis important to guarantee the health of customers. the aim of this study was to evaluate the sanitary quality of food services by checking the presence of thermotolerant coliforms, staphylococcus sp. and evaluate the toxigenic potential from the latter. the analysis was performed on water, surfaces, equipment, ready-to-eat foods, hands and nasal cavity of handlers in seven food services. the wat ... | 2014 | 25477940 |
| comparative proteomic and transcriptomic profile of staphylococcus epidermidis biofilms grown in glucose-enriched medium. | staphylococcus epidermidis is an important nosocomial agent among carriers of indwelling medical devices, due to its strong ability to form biofilms on inert surfaces. contrary to some advances made in the transcriptomic field, proteome characterization of s. epidermidis biofilms is less developed. to highlight the relation between transcripts and proteins of s. epidermidis biofilms, we analyzed the proteomic profile obtained by two mechanical lysis methods (sonication and bead beating), associa ... | 2015 | 25476368 |
| coagulase-negative staphylococci (cons) isolated from ready-to-eat food of animal origin--phenotypic and genotypic antibiotic resistance. | the aim of this work was to study the pheno- and genotypical antimicrobial resistance profile of coagulase negative staphylococci (cons) isolated from 146 ready-to-eat food of animal origin (cheeses, cured meats, sausages, smoked fishes). 58 strains were isolated, they were classified as staphylococcus xylosus (n = 29), staphylococcus epidermidis (n = 16); staphylococcus lentus (n = 7); staphylococcus saprophyticus (n = 4); staphylococcus hyicus (n = 1) and staphylococcus simulans (n = 1) by phe ... | 2015 | 25475289 |
| detection of necrosis of the gastric fundus after blunt abdominal trauma by pet-ct. | positron emission tomography with [(18)f]-fluorodeoxyglucose provides functional and anatomic information by visualising the uptake of radiolabelled glucose in tumour and inflammatory cells. we report delayed diagnosis of necrosis of the gastric fundus after blunt abdominal trauma in a 73-year-old man. after a car accident with head-on collision, the patient was stabilised in our emergency room. his femur was treated by internal fixation, his ellbow was stabilised by a fixateur externe. during s ... | 2015 | 25421368 |
| reduced susceptibility to vancomycin and biofilm formation in methicillin-resistant staphylococcus epidermidis isolated from blood cultures. | this study aimed to correlate the presence of ica genes, biofilm formation and antimicrobial resistance in 107 strains of staphylococcus epidermidis isolated from blood cultures. the isolates were analysed to determine their methicillin resistance, staphylococcal cassette chromosome mec (sccmec) type, ica genes and biofilm formation and the vancomycin minimum inhibitory concentration (mic) was measured for isolates and subpopulations growing on vancomycin screen agar. the meca gene was detected ... | 2014 | 25410990 |
| human milk bactericidal properties: effect of lyophilization and relation to maternal factors and milk components. | lyophilization appears to be a viable method for storing human milk, assuring no microbiological contamination and preserving its health benefits and antibacterial properties. the aim of the study is to evaluate and compare the effects of different storage methods (lyophilization and freezing at -20°c and -80°c) and maternal factors (gestational length or time postpartum) upon the microbiological contents and bactericidal activity of human milk. the possible relation between bactericidal activit ... | 2015 | 25406523 |
| staphylococcus epidermidis srrab regulates bacterial growth and biofilm formation differently under oxic and microaerobic conditions. | srrab expression in staphylococcus epidermidis strain 1457 (se1457) was upregulated during a shift from oxic to microaerobic conditions. an srra deletion (δsrra) mutant was constructed for studying the regulatory function of srrab. the deletion resulted in retarded growth and abolished biofilm formation both in vitro and in vivo and under both oxic and microaerobic conditions. associated with the reduced biofilm formation, the δsrra mutant produced much less polysaccharide intercellular adhesion ... | 2015 | 25404696 |
| the effect of iatrogenic staphylococcus epidermidis intercellar adhesion operon on the formation of bacterial biofilm on polyvinyl chloride surfaces. | the intercellular adhesion gene (ica) of staphylococcus epidermidis is a key factor for bacterial aggregation. this study explored the effect of ica on the formation of bacterial biofilm on polyvinyl chloride (pvc) surfaces. | 2014 | 25402758 |
| whole cerebrospinal axis infection after lumbar epidural injection: a case report. | lumbar epidural injection is a popular treatment for degenerative lumbar disease. although post-procedural epidural infection is rare, meningitis and epidural abscess are life-threatening conditions, and need additional medical and surgical intervention. the purpose of this article is to report a patient with fatal whole cerebrospinal axis infection after lumbar epidural injections. | 2015 | 25391626 |
| complete genome assembly of staphylococcus epidermidis amms 205. | staphylococcus epidermidis causes a large number of catheter-related sepsis infections annually in the united states. we present the 2.54-mbp complete genome assembly of reference strain s. epidermidis amms 205, including a single 37.7-kbp plasmid. the annotated assembly is available in genbank under accession numbers cp009046 and cp009047. | 2014 | 25377697 |
| acute and chronic staphylococcus epidermidis post-operative endophthalmitis: the importance of biofilm production. | to report two cases of acute and chronic-onset, postoperative staphylococcus epidermidis endophthalmitis and discuss the virulence and treatment of this saprophytic pathogen. after clinical diagnosis of endophthalmitis, prompt vitreous culture was performed with injection of intravitreal vancomycin 1 mg/0.1 ml and ceftazidime 2.25 mg/0.1 ml. with no improvement after this procedure, a pars plana vitrectomy and lensectomy were performed to further decrease microbial load and repeat the intravitre ... | 2014 | 25369889 |
| pathology image of the month: death in a young adult with sickle cell disease. | a 27-year-old african-american female with known sickle cell disease was admitted for sickle cell crisis and presumed sepsis. the patient's past medical history was complicated by multiple sickle cell-related complications, including seizures and multiple prior blood transfusions. her hospital course included staphylococcus epidermidis bacteremia, for which broad spectrum antibiotics were prescribed. on hospital day nine, the patient was found unresponsive and declared dead after unsuccessful ef ... | 2017 | 25369229 |
| preparation and pharmaceutical evaluation of nicotinamide stick for eradication of staphylococcus epidermidis. | staphylococcus epidermidis is a part of the skin's normal flora that can cause acne. this study was designed to evaluate the efficacy of nicotinamide as a stick in eradication of staphylococcus. | 2014 | 25364367 |
| [construction and functional analysis of mscl knockout mutant of staphylococcus epidermidis]. | to investigate the biological function of mscl gene in s. epidermidis. | 2014 | 25345936 |
| reduced susceptibility to vancomycin and biofilm formation in methicillin-resistant staphylococcus epidermidis isolated from blood cultures. | this study aimed to correlate the presence of ica genes, biofilm formation and antimicrobial resistance in 107 strains of staphylococcus epidermidis isolated from blood cultures. the isolates were analysed to determine their methicillin resistance, staphylococcal cassette chromosome mec (sccmec) type, ica genes and biofilm formation and the vancomycin minimum inhibitory concentration (mic) was measured for isolates and subpopulations growing on vancomycin screen agar. the meca gene was detected ... | 2014 | 25338149 |
| accumulation-associated protein enhances staphylococcus epidermidis biofilm formation under dynamic conditions and is required for infection in a rat catheter model. | biofilm formation is the primary virulence factor of staphylococcus epidermidis. s. epidermidis biofilms preferentially form on abiotic surfaces and may contain multiple matrix components, including proteins such as accumulation-associated protein (aap). following proteolytic cleavage of the a domain, which has been shown to enhance binding to host cells, b domain homotypic interactions support cell accumulation and biofilm formation. to further define the contribution of aap to biofilm formatio ... | 2015 | 25332125 |
| commensal bacteria and cutaneous immunity. | the skin is the human body's largest organ and is home to a diverse and complex variety of innate and adaptive immune functions that protect against pathogenic invasion. recent studies have demonstrated that cutaneous commensal bacteria modulated the host immune system. for example, staphylococcus epidermidis, a skin commensal bacterium, has been demonstrated to induce cutaneous interferon (ifn)-γ- and interleukin (il)-17a-producing t cells. in addition, cutaneous microbiota changes occur in the ... | 2015 | 25326105 |
| a rare case of splenic abscess with septic peritonitis in a german shepherd dog. | splenic abscess is a rare disease with only few reports in small-animal practice as well as in human medicine. it has been mostly reported in immunocompromised patients or following penetrating foreign bodies. this report aims to add to the current veterinary literature on recommended diagnostic tools for splenic abscess, as well as to provide follow-up findings after successful surgical treatment. | 2014 | 25319320 |
| daptomycin-associated eosinophilic pneumonia in two patients with prosthetic joint infection. | daptomycin is used increasingly to treat prosthetic joint infection (pji). a possible side effect of this drug is eosinophilic pneumonia. we describe two patients with pji treated with daptomycin who had this side effect with different clinical presentations. | 2014 | 25317960 |
| characterization of a novel plasmid-borne thiopeptide gene cluster in staphylococcus epidermidis strain 115. | thiopeptides are small (12- to 17-amino-acid), heavily modified peptides of bacterial origin. this antibiotic family, with more than 100 known members, is characterized by the presence of sulfur-containing heterocyclic rings and dehydrated residues within a macrocyclic peptide structure. thiopeptides, including micrococcin p1, have garnered significant attention in recent years for their potent antimicrobial activity against bacteria, fungi, and even protozoa. micrococcin p1 is known to target t ... | 2014 | 25313391 |
| double-barrel proximal thoracic aorta or horrific complication of endocarditis? | | 2015 | 25306518 |
| characterization of staphylococcus epidermidis phage vb_seps_sep9 - a unique member of the siphoviridae family. | relatively few phages (<10) of coagulase negative staphylococci (cons) have been described. staphylococcus epidermidis phage vb_seps_sep9 is a siphovirus with a unique morphology as a staphylococcal phage, possessing a very long tail. its genome is unique and unrelated to any phage genomes deposited in public databases. it appears to encode a nonfunctional integrase. due to the not having a recognizable lysogeny module, the phage is unable lysogenize. the genome comprises 129 coding sequences (c ... | 2014 | 25303835 |
| methicillin resistant staphylococcus epidermidis induced endogenous endophthalmitis. | fulminant endogenous endophthalmits, a relatively rare intraocular infection, caused by haematogenous spread of bacteria or fungi is usually seen in immune-deficient patients. we report a case of methicillin resistant staphylococcus epidermidis causing endogenous endophthalmitis following platelet transfusion and intravenous antibiotic therapy for a suspected dengue fever. | 2014 | 25302249 |
| early staphylococcal biofilm formation on solid orthopaedic implant materials: in vitro study. | biofilms forming on the surface of biomaterials can cause intractable implant-related infections. bacterial adherence and early biofilm formation are influenced by the type of biomaterial used and the physical characteristics of implant surface. in this in vitro research, we evaluated the ability of staphylococcus epidermidis, the main pathogen in implant-related infections, to form biofilms on the surface of the solid orthopaedic biomaterials, oxidized zirconium-niobium alloy, cobalt-chromium-m ... | 2014 | 25299658 |
| in vitro biological activity of aromadendrin-4'-methyl ether isolated from root extract of ventilago madraspatana gaertn with relevance to anticandidal activity. | in this study, antimicrobial and antioxidant activities of the hexane extract of the root of ventilago madraspatana were evaluated. based on the significant bioactivity of crude hexane extract, an active compound was purified from the root extract. the active compound was further purified and identified as aromodendrin-4'-methyl ether by the (1)h nmr spectrum. the isolated compound significantly inhibited staphylococcus epidermidis with the lowest mic and mbc at 78 μg/ml (p < 0.05). the compound ... | 2015 | 25299472 |
| novel microcalorimetric assay for antibacterial activity of implant coatings: the cases of silver-doped hydroxyapatite and calcium hydroxide. | biomaterials with antimicrobial properties are now commonly used in different clinical specialties including orthopedics, endodontic, and traumatology. as a result, assessing the antimicrobial effect of coatings applied on implants is of critical importance. in this study, we demonstrate that isothermal microcalorimetry (imc) can be used for monitoring bacterial growth and biofilm formation at the surface of such coatings and for determining their antimicrobial effects. the antibacterial effects ... | 2015 | 25288132 |
| clinical characteristics of staphylococcus epidermidis: a systematic review. | staphylococci are known as clustering gram-positive cocci, nonmotile, non-spore forming facultatively anaerobic that classified in two main groups, coagulase-positive and coagulase-negative. staphylococcus epidermidis with the highest percentage has the prominent role among coagulase-negative staphylococci that is the most important reason of clinical infections. due to various virulence factors and unique features, this microorganism is respected as a common cause of nosocomial infections. beca ... | 2014 | 25285267 |
| extracellular dna impedes the transport of vancomycin in staphylococcus epidermidis biofilms preexposed to subinhibitory concentrations of vancomycin. | staphylococcus epidermidis biofilm formation is responsible for the persistence of orthopedic implant infections. previous studies have shown that exposure of s. epidermidis biofilms to sub-mics of antibiotics induced an increased level of biofilm persistence. bodipy fl-vancomycin (a fluorescent vancomycin conjugate) and confocal microscopy were used to show that the penetration of vancomycin through sub-mic-vancomycin-treated s. epidermidis biofilms was impeded compared to that of control, untr ... | 2014 | 25267673 |
| role for the a domain of unprocessed accumulation-associated protein (aap) in the attachment phase of the staphylococcus epidermidis biofilm phenotype. | the polysaccharide intercellular adhesin or the cell wall-anchored accumulation-associated protein (aap) mediates cellular accumulation during staphylococcus epidermidis biofilm maturation. mutation of sortase, which anchors up to 11 proteins (including aap) to the cell wall, blocked biofilm development by the cerebrospinal fluid isolate csf41498. aap was implicated in this phenotype when western blots and two-dimensional (2d) electrophoresis revealed increased levels of the protein in culture s ... | 2014 | 25266380 |
| biofilm eradication kinetics of the ultrashort lipopeptide c12 -ooww-nh2 utilizing a modified mbec assay(™). | in this study, we report the antimicrobial planktonic and biofilm kill kinetics of ultrashort cationic lipopeptides previously demonstrated by our group to have a minimum biofilm eradication concentration (mbec) in the microgram per ml (μg/ml) range against clinically relevant biofilm-forming micro-organisms. we compare the rate of kill for the most potent of these lipopeptides, dodecanoic (lauric) acid-conjugated c12 -orn-orn-trp-trp-nh2 against the tetrapeptide amide h-orn-orn-trp-trp-nh2 moti ... | 2015 | 25264285 |
| role of the two-component regulatory system arlrs in ica operon and aap positive but non-biofilm-forming staphylococcus epidermidis isolates from hospitalized patients. | the ica operon and aap gene are important factors for staphylococcus epidermidis biofilm formation. however, we found 15 out of 101 s. epidermidis strains isolated from patients had both the ica operon and the aap gene in the genome but could not form biofilms (ica(+)aap(+)/bf(-) isolates). compared with standard strain rp62a, the 15 ica(+)aap(+)/bf(-) isolates had similar growth curves and initial attachment abilities, but had much lower apparent transcription levels of the icaa gene and signif ... | 2014 | 25263000 |
| antibacterial activity of pinus elliottii and its major compound, dehydroabietic acid, against multidrug-resistant strains. | antibiotic-resistant bacteria have emerged from the widespread use of antibiotics worldwide and have prompted the search for new sources of antimicrobial substances. pinus spp. contain several bioactive compounds consisting mainly of terpenes, terpenoids and some other aromatic and aliphatic constituents. these compounds exert important biological effects, and pine oils have found wide application in the industry. in the present study, we have evaluated the potential activity of the resin-oil of ... | 2014 | 25261060 |
| multi drug resistance in strong biofilm forming clinical isolates of staphylococcus epidermidis. | staphylococcus epidermidis which exists in healthy human skin as a commensal inhabitant is also an important pathogen forming biofilms on many surfaces and recently, increased resistance traits were suggested to be acquired in biofilm environments. in this study; clinical prevalences, antibiotic resistances and biofilm formations of s. epidermidis strains were determined and comparison of all these findings with each other was carried out in order to take precautions against them and figure out ... | 2014 | 25242939 |
| first report on the molecular epidemiology of malaysian staphylococcus epidermidis isolated from a university teaching hospital. | staphylococcus epidermidis is a pathogen associated with nosocomial infections whose medical importance has increased due to progressively invasive medical procedures. in this study, we characterized the molecular epidemiology of s. epidermidis strains circulating in our university hospital situated in kuala lumpur, malaysia. | 2014 | 25186825 |
| effect of surface roughness of biomaterials on staphylococcus epidermidis adhesion. | implant-related infections are caused by adhesion of bacteria to the surface of biomaterials. in this in vitro research, we evaluated the ability of staphylococcus epidermidis (atcc35984) to adhere to the surface of solid biomaterials at different levels of roughness below 30 nm ra and investigated the minimum level of roughness required to promote bacterial adhesion on five kinds of biomaterials: oxidized zirconium-niobium alloy (oxinium), cobalt-chromium-molybdenum alloy (co-cr-mo), titanium a ... | 2014 | 25179448 |
| conditional tolerance of temperate phages via transcription-dependent crispr-cas targeting. | a fundamental feature of immune systems is the ability to distinguish pathogenic from self and commensal elements, and to attack the former but tolerate the latter. prokaryotic crispr-cas immune systems defend against phage infection by using cas nucleases and small rna guides that specify one or more target sites for cleavage of the viral genome. temperate phages include viruses that can integrate into the bacterial chromosome, and they can carry genes that provide a fitness advantage to the ly ... | 2014 | 25174707 |
| [development of hospital pharmacy practice using therapeutic drug monitoring]. | therapeutic drug monitoring (tdm) is a developing field in hospital pharmacy that contributes to good clinical response. optimal concentrations of immunosuppressants, anti-epileptic drugs, and glycopeptide antibiotics are narrow, and these drugs are widely monitored by fluorescence polarization immunoassay (fpia) in clinical settings. recently, a chemiluminescence immunoassay (clia) has also become available. we compared these two assays in monitoring serum concentrations of cyclosporin a (csa), ... | 2014 | 25174365 |
| extracellular biosynthesis of zinc oxide nanoparticles using rhodococcus pyridinivorans nt2: multifunctional textile finishing, biosafety evaluation and in vitro drug delivery in colon carcinoma. | in this study, zinc oxide (zno) nanoparticles (nps) were rapidly synthesized from zinc sulfate solution at room temperature using a metabolically versatile actinobacteria rhodococcus pyridinivorans nt2. the morphology, structure and stability of the synthesized zno nps were studied using uv-visible absorption spectroscopy, x-ray diffraction (xrd), fourier transform infrared (ftir) spectroscopy, field emission scanning electron microscopy (fesem) with energy dispersive spectroscopy (eds), transmi ... | 2014 | 25169770 |
| autoimmune aspects of giant cell arteritis. | giant cell arteritis (gca) is considered to be a t cell-dependent disease. autoantibodies have not consistently been found in gca. the exception is antiphospholipid antibodies (apla), which were found in 30-80% of gca cases. recently, efforts have been made to seek autoantibodies in gca using newer methods of detection: serological identification of antigens by recombinant cdna expression cloning, and a proteomic approach. in these studies, lamin c (a nuclear envelope antigen) was recognized by ... | 2014 | 25167695 |
| infection related glomerulonephritis associated with staphylococcus epidermidis in the absence of prosthetic material. | we report a case of a 72-year-old diabetic male who developed infection-related glomerulonephritis (irgn) in the setting of severe staphylococcus epidermidis infection. he required renal replacement therapy for 6 weeks, but had full recovery of his kidney function with aggressive treatment of the infection. while this pathogen has been previously implicated as the cause of shunt nephritis, it is exceptionally rare to be associated with irgn in the absence of a shunt or other prosthetic material. | 2014 | 25161783 |
| staphylococcus epidermidis originating from titanium implants infects surrounding tissue and immune cells. | infection is a major cause of failure of inserted or implanted biomedical devices (biomaterials). during surgery, bacteria may adhere to the implant, initiating biofilm formation. bacteria are also observed in and recultured from the tissue surrounding implants, and may even reside inside host cells. whether these bacteria originate from biofilms is not known. therefore, we investigated the fate of staphylococcus epidermidis inoculated on the surface of implants as adherent planktonic cells or a ... | 2014 | 25153780 |