| molecular cloning and expression of the gene encoding adp-glucose pyrophosphorylase from the cyanobacterium anabaena sp. strain pcc 7120. | previous studies have indicated that adp-glucose pyrophosphorylase (adpglc ppase) from the cyanobacterium anabaena sp. strain pcc 7120 is more similar to higher-plant than to enteric bacterial enzymes in antigenicity and allosteric properties. in this paper, we report the isolation of the anabaena adpglc ppase gene and its expression in escherichia coli. the gene we isolated from a genomic library utilizes gtg as the start codon and codes for a protein of 48,347 da which is in agreement with the ... | 1992 | 1325205 |
| nasal mucosa sensitization with toluene diisocyanate (tdi) increases preprotachykinin a (ppta) and preprocgrp mrnas in guinea pig trigeminal ganglion neurons. | toluene diisocyanate (tdi) induces respiratory allergy in mammals. using immunohistochemistry and in situ hybridization histochemistry, the present study examined effects of nasal mucosa sensitization by tdi on the immunoreactivity for substance p (sp) and calcitonin gene-related peptide (cgrp) and on the expression of their mrnas in guinea pig trigeminal ganglion and their terminals. single intranasal application of tdi (acute experiment) did not induce nasal allergy-like behaviours and failed ... | 1992 | 1325240 |
| ws009 a and b, new endothelin receptor antagonists isolated from streptomyces sp. no. 89009. i. taxonomy, fermentation, isolation, physico-chemical properties and biological activities. | ws009 a and b novel endothelin receptor antagonists, have been isolated from the fermentation broth of streptomyces sp. no. 89009. these antagonists were purified from the culture filtrate followed by diaion sp-207, deae toyopearl column chromatography and hplc. ws009 a and b showed selective activity in an endothelin receptor binding assay with ic50 of 5.8 x 10(-6) m and 6.7 x 10(-7) m, respectively. on the basis of spectroscopic and chemical evidence, the structures of ws009 a and b have been ... | 1992 | 1325433 |
| ws009 a and b, new endothelin receptor antagonists isolated from streptomyces sp. no. 89009. ii. biological characterization and pharmacological characterization of ws009 a and b. | ws009 a and b, produced by streptomyces sp. no. 89009, were found to be competitive and specific antagonists against endothelin (et)-1 receptors in in vitro studies and also active in in vivo studies. furthermore, ws009 a and b were specific antagonists for vascular et-1 receptors (eta receptors) and significantly prevented the accumulation of intracellular inositol 1,4,5-triphosphate (ip3) in endothelin treated rat aorta tissues. | 1992 | 1325434 |
| uct4b, a new antitumor antibiotic with topoisomerase ii mediated dna cleavage activity, from streptomyces sp. | | 1992 | 1325436 |
| rat surfactant protein d enhances the production of oxygen radicals by rat alveolar macrophages. | rat surfactant protein d (sp-d) was shown to enhance the production of oxygen radicals by rat alveolar macrophages. this enhancement, which was determined by a lucigenin-dependent chemiluminescence assay, was maximal after 18 min at an sp-d concentration of 0.2 micrograms/ml. surfactant lipids did not influence the stimulation of alveolar macrophages by sp-d, whereas the oxygen-radical production of these cells induced by surfactant protein a was inhibited by the lipids in a concentration-depend ... | 1992 | 1325783 |
| kinetic properties of electrogenic na+/h+ antiport in membrane vesicles from an alkalophilic bacillus sp. | the effects of imposed proton motive force on the kinetic properties of the alkalophilic bacillus sp. strain n-6 na+/h+ antiport system have been studied by looking at the effect of delta psi (membrane potential, interior negative) and/or delta ph (proton gradient, interior alkaline) on na+ efflux or h+ influx in right-side-out membrane vesicles. imposed delta psi increased the na+ efflux rate (v) linearly, and the slope of v versus delta psi was higher at ph 9 than at ph 8. kinetic experiments ... | 1992 | 1325968 |
| organization, structure, chromosomal assignment, and expression of the gene encoding the human endothelin-a receptor. | we have isolated and characterized the gene for the human endothelin-a receptor. southern blot analyses demonstrated a single copy gene for the receptor. the gene spans more than 40 kilobases and contains eight exons and seven introns. intron 1 exists in the 5'-noncoding region, and introns 2-7 occur in the coding region. the locations of introns 2-7 exist before or after the regions encoding the membrane-spanning domains. the transcription start site, determined by primer extension experiments, ... | 1992 | 1326535 |
| nonadrenergic, noncholinergic contractile responses of the guinea pig hilar bronchus involve the preferential activation of tachykinin neurokinin2 receptors. | experiments were designed to characterize receptor(s) that mediate nonadrenergic, noncholinergic contractions of the guinea pig hilar bronchus using selective neurokinin (nk)1 (cp 96,345) and nk2 (r396 and men 10,376) tachykinin receptor antagonists. left and right hilar bronchi were studied as pairs in the presence of atropine, propranolol, phentolamine; indomethacin and thiorphan. (2s, 3s)-cis-2-(diphenylmethyl)-n-(2-methoxyphe nyl)-1-azabicyclo[2,2,2]octan-3-amine (cp 96,345) selectively anta ... | 1992 | 1326630 |
| mapping of homologous, amino-terminal neutralizing regions of human t-cell lymphotropic virus type i and ii gp46 envelope glycoproteins. | twelve synthetic peptides containing hydrophilic amino acid sequences of human t-cell lymphotropic virus type i (htlv-i) envelope glycoprotein were coupled to tetanus toxoid and used to raise epitope-specific antisera in goats and rabbits. low neutralizing antibody titers (1:10 to 1:20) raised in rabbits to peptides sp-2 (envelope amino acids [aa] 86 to 107), sp-3 (aa 176 to 189), and sp-4a (aa 190 to 209) as well as to combined peptide sp-3/4a (aa 176 to 209) were detected in the vesicular stom ... | 1992 | 1326649 |
| applications of anti-curosurf antibodies to understanding the biology of alveolar surfactant. | the structure of surfactant-binding proteins from alveolar cell membranes was investigated using monoclonal anti-idiotypic antibodies directed against surfactant-binding regions of anti-surfactant monoclonal antibodies. two different series of monoclonal anti-surfactant antibodies were used. one series included antibodies against rabbit surfactant, and the other antibodies against porcine surfactant. in addition, two monoclonal anti-idiotype antibodies were made. these anti-idiotype antibodies, ... | 1992 | 1327194 |
| mutational analysis of genes determining antagonism of alcaligenes sp. strain mfa1 against the phytopathogenic fungus fusarium oxysporum. | alcaligenes sp. strain mfa1 inhibits microconidial germination and germination-tube elongation of fusarium oxysporum f.sp. dianthi and reduces the severity of fusarium wilt of carnation, presumably as a result of its production of a siderophore (g.y. yuen and m.n. schroth. 1986. phytopathology, 76:171-176). derivative strains of mfa1, deficient in antagonism against f. oxysporum and in iron-limited growth, were obtained by tn5 mutagenesis. the presence of a single tn5 insertion in the genomic dn ... | 1992 | 1327482 |
| evidence that an atypical beta-adrenoceptor mediates the prejunctional inhibition of non-adrenergic non-cholinergic contraction in guinea-pig bronchi. | we investigated the effect of the putative beta 3 agonist brl 35135 on non-adrenergic non-cholinergic (nanc) contractions in guinea-pig bronchial strips. brl 35135 (10(-9) to 10(-6) m) did not alter the baseline tension but reduced nanc contractions induced by electrical field stimulation (efs) in a concentration-dependent fashion without having a significant effect on the contraction induced by substance p (10(-6) m). brl 35135 (10(-6) m) also reduced the contraction induced by capsaicin (10(-7 ... | 1992 | 1327825 |
| novel streptococcal-integration shuttle vectors for gene cloning and inactivation. | seven new streptococcal integration shuttle vectors have been constructed which contain different antibiotic-resistance-encoding genes capable of expression in both streptococcus sp. and escherichia coli. these plasmids can replicate in e. coli, but not in streptococci because of the absence of a streptococcal origin of replication. the size, antibiotic resistance, and number of unique restriction sites available for cloning for each plasmid are as follows: psf141 (7.6 kb, cmr and kmr, 7 sites), ... | 1992 | 1327968 |
| gastrointestinal symptoms in patients infected with human immunodeficiency virus: relevance of infective agents isolated from gastrointestinal tract. | the correlation of gastrointestinal symptoms and infections in 186 consecutive patients with human immunodeficiency virus (hiv) infection undergoing diagnostic endoscopy (oesophagogastroduodenoscopy, n = 124; colonoscopy, n = 37; both, n = 25) was investigated. biopsy and stool samples were examined for infective agents. only weight loss (p = 0.003) and dysphagia (p = 0.027) were more common in patients at stage cdc iv compared with earlier stages. in three of 27 patients at stage ii/iii and in ... | 1992 | 1327982 |
| lack of control of liver gluconeogenesis in cholestatic rats with reduced portal blood flow. | previous studies indicated a role for ischemia in the metabolic changes induced by cholestasis. liver pyruvate kinase is a key enzyme for the concurrent control of glycolysis and gluconeogenesis. in this experiment the control of pyruvate kinase activity was investigated in cholestatic rats. pyruvate kinase kinetics changed from a sigmoidal type in sham-operated rats to a hyperbolic type in obstructed rats. the change in the enzymatic kinetics paralleled the reduction in the portal blood flow, w ... | 1992 | 1328007 |
| functional characterization of neurokinin-1 receptors on human u373mg astrocytoma cells. | the neurokinin-1 (nk-1, substance p) receptor belongs to the class of seven transmembrane domain (7-tm) receptors that interact with cellular effector systems via guanine nucleotide binding regulatory proteins (g-proteins). in this study, coupling mechanisms of functional nk-1 receptors endogenously expressed in a human astrocytoma cell line (u373mg) were analyzed. stimulation with substance p (sp) resulted in 1) a rapid increase in inositol 1,4,5-trisphosphate (ip3) synthesis; 2) a rise in cyto ... | 1992 | 1328053 |
| design of chimeric peptide ligands to galanin receptors and substance p receptors. | several chimeric peptides were synthesized and found to be high-affinity ligands for both galanin and substance p receptors in membranes from the rat hypothalamus. the peptide galantide, composed of the n-terminal part of galanin and c-terminal part of substance p (sp), galanin-(1-12)-pro-sp-(5-11) amide, which is the first galanin antagonist to be reported, recognizes two classes of galanin binding sites (kd(1) less than 0.1 nm and kd(2) approximately 6 nm) in the rat hypothalamus, while it app ... | 1992 | 1328099 |
| an inhibitor for inositol-specific phospholipase c from actinomadura sp. | | 1992 | 1328124 |
| synthesis of nitrogenase in mutants of the cyanobacterium anabaena sp. strain pcc 7120 affected in heterocyst development or metabolism. | mutants of anabaena sp. strain pcc 7120 that are incapable of sustained growth with air as the sole source of nitrogen were generated by using tn5-derived transposons. nitrogenase was expressed only in mutants that showed obvious morphological signs of heterocyst differentiation. even under rigorously anaerobic conditions, nitrogenase was not synthesized in filaments that were unable to develop heterocysts. these results suggest that competence to synthesize nitrogenase requires a process that l ... | 1992 | 1328150 |
| characterization of tn5 mutants deficient in dissimilatory nitrite reduction in pseudomonas sp. strain g-179, which contains a copper nitrite reductase. | tn5 was used to generate mutants that were deficient in the dissimilatory reduction of nitrite for pseudomonas sp. strain g-179, which contains a copper nitrite reductase. three types of mutants were isolated. the first type showed a lack of growth on nitrate, nitrite, and nitrous oxide. the second type grew on nitrate and nitrous oxide but not on nitrite (nir-). the two mutants of this type accumulated nitrite, showed no nitrite reductase activity, and had no detectable nitrite reductase protei ... | 1992 | 1328160 |
| mitochondrial porin can be translocated across both endoplasmic reticulum and mitochondrial membranes. | mitochondrial porin is a major integral membrane protein of the outer membrane. to assess the stop-transfer sequence in the yeast porin molecule (p), we constructed the following chimeric proteins. (i) the signal sequence of interleukin 2, a secretory protein, was fused to the amino-terminus of porin (sp). (ii) the matrix targeting presequence of cytochrome c oxidase subunit iv was fused to the amino-terminus of porin (cp). (iii) the amino-terminal segment consisting of 42 amino acid residues of ... | 1992 | 1328170 |
| processing of delta-endotoxin from bacillus thuringiensis subsp. kurstaki hd-1 and hd-73 by gut juices of various insect larvae. | midgut juices were prepared from adoxophyes sp., smaller tea tortrix (stt); bombyx mori, silkworm (sw); spodoptera litura, common cutworm (ccw); plutella xylostella, diamondback moth (dbm); and musca domestica, housefly (hf) and immobilized onto sepharose 4b. delta-endotoxins (icps) from bacillus thuringiensis subsp. kurstaki hd-1 and hd-73 were digested by these immobilized gut juice proteases. all gut juices tested derived relatively proteolytic resistant cores from icp. the molecular sizes of ... | 1992 | 1328398 |
| comparative in vitro activity of sparfloxacin (at 4140, rp 64206--spfx) against 275 multiresistant clinical isolates. | the in-vitro activity of sparfloxacin was compared with that of pefloxacin, ofloxacin, ciprofloxacin, imipenem, ceftazidime, gentamicin and amikacin against 275 multiresistant nosocomial clinical isolates. they consisted of pseudomonas aeruginosa (37), enterobacter cloacae (42), acinetobacter anitratus (60), klebsiella pneumoniae (37) and staphylococcus sp (99). minimum inhibitory concentrations (mic90) and geometric mean mics for sparfloxacin were as follows (mg/l): p. aeruginosa 128-23.7, e. c ... | 1992 | 1328554 |
| immunohistochemical localization of receptors for vasoactive intestinal peptide and substance p in human trachea. | background: tissue sections of human cervical trachea were processed for immunohistochemical demonstration of receptors for substance p [using an anti-sp anti-idiotypic antiserum directed toward the ligand binding site of the receptor (couraud j-y, escher ed, regoli d, imhof v, rossignol b, pradelles p. anti-substance p anti-idiotypic antibodies: characterization and biological activities. j biol chem 1985;260:9461-9; couraud j-y, maillet s, grassi j, frobert y, pradelles p. characterization and ... | 1992 | 1328764 |
| role of nk-1 receptor in central cardiovascular regulation in rats: studies on a novel non-peptide antagonist, cp-96,345, of substance p nk-1 receptor. | cp-96,345[(2s,3s)-cis-2-(diphenylmethyl)-n-[(2-methoxyphenyl)- methyl]-1-azabicyclo [2.2.2] octan-3-amine] was recently discovered to be a nonpeptide substance p (sp) antagonist. we examined the effects of cp-96,345 on the central cardiovascular responses to tachykinin peptides in anesthetized rats. cp-96,345 (200 nmol, i.c.v.) inhibited the pressor responses of the nk-1 receptor-selective agonist gr 73632 (0.5 nmol, i.c.v.) and sp (7 nmol, i.c.v.). it also inhibited the increase in blood pressu ... | 1992 | 1328962 |
| [a comparative study of the physico-chemical properties of lysine oxidase from trichoderma sp. and trichoderma viride y 244-2]. | some physicochemical properties of l-lysine oxidase from two strains of trichoderma were studied. content of metals and cofactors (se, zn, cu, fe, co, mn, mo), amino acid analysis, secondary structure were estimated. the enzyme molecule from trichoderma sp was found to contain both fad and pqq cofactors. | 1992 | 1329350 |
| antimicrobial activities of two investigational fluoroquinolones (ci-960 and e4695) against over 100 legionella sp. isolates. | the antimicrobial activities of two investigational fluoroquinolones (ci-960 and e4695) were compared with those of five similar compounds and four comparison drugs against 103 strains of legionella pneumophila and five other legionella species type strains. when concentrations inhibiting 90% of strains tested (mic90s) for l. pneumophila were determined, ci-960 and temafloxacin emerged as the most active (0.015 microgram/ml) and were followed in potency by e4695 (0.03 microgram/ml). this activit ... | 1992 | 1329642 |
| selenoproteins in mitochondria and cytosol of saccharomyces uvarum after growth in sodium selenite-supplemented media. | the extent of the biosynthesis of selenoproteins in mitochondria and cytosol of saccharomyces uvarum depends on the sodium selenite concentration in the medium. in mitochondria there is a selenoprotein (sp 1) exhibiting glutathione peroxidase activity whose concentration already reaches a maximum at low concentrations of sodium selenite. a second selenoprotein (sp 2) was found in mitochondria and cytosol. both proteins contain l-selenocysteine. the molecular masses of sp 1 and sp 2 are 73,000 da ... | 1992 | 1330131 |
| changes in intrinsic inhibition in isolated hippocampal slices during ethanol withdrawal; lack of correlation with withdrawal hyperexcitability. | 1. intracellular recordings were made from pyramidal cells in area ca1 in mouse isolated hippocampal slices, after chronic ethanol treatment in vivo. 2. fast i.p.s.ps were isolated by injection of the impaled neurones with qx314 (to block fast sodium currents and the slow i.p.s.p.) and stimulating the interneurones in the presence of the glutamatergic blockers, cnqx and apv. 3. the isolated fast-inhibitory postsynaptic potential (f.-i.p.s.p.) was measured at intervals during the 7 h withdrawal p ... | 1992 | 1330182 |
| antiinflammatory and analgesic activity of a non-peptide substance p receptor antagonist. | cp-96,345, a potent non-peptide antagonist of the substance p (sp) receptor, inhibited sp-, neurokinin a (nka)- and neurokinin b-induced plasma extravasation in guinea pig dorsal skin. the inhibition was specific for the three tachykinins; cp-96,345 was not active against plasma leakage caused by histamine, bradykinin, platelet-activating factor or leukotriene d4. cp-96,345 inhibited capsaicin-induced plasma extravasation in the ureter, an inflammatory response caused by neuropeptides released f ... | 1992 | 1330589 |
| analgesia by direct antagonism of nociceptor sensitization involves the arginine-nitric oxide-cgmp pathway. | we tested the hypothesis that activation of the nitric oxide (no)-cgmp pathway is involved in the mechanism of two directly acting non-opiate peripheral analgesics, myrcene and dipyrone, using our modification of the randall-selitto test. the no inhibitor, ng-monomethyl-l-arginine (50 micrograms/paw) and methylene blue (500 micrograms/paw) abolished the analgesic effect of dipyrone and myrcene. dibutyryl cyclic adenosine monophosphate (dbcamp) caused a dose-dependent hyperalgesia (20, 50 and 100 ... | 1992 | 1330593 |
| the aryldialkylphosphatase-encoding gene adpb from nocardia sp. strain b-1: cloning, sequencing and expression in escherichia coli. | using degenerate oligodeoxyribonucleotides (oligos) derived from the n-terminal sequence of an aryldialkylphosphatase (adpase) from nocardia sp. strain b-1, an amplification reaction was used to isolate a dna segment containing a 57-bp fragment from the adpb gene. based on the nucleotide (nt) sequence of this fragment, a nondegenerate oligo was synthesized and used to screen a subgenomic library of strain b-1 dna for fragments containing adpb. a 3.55-kb psti fragment containing adpb was cloned i ... | 1992 | 1330830 |
| conformationally constrained tachykinin analogues: potent and highly selective neurokinin nk-2 receptor agonists. | the design and synthesis of potent and selective neurokinin nk-2 receptor agonists 12 (gr64349) and 31 are described, together with structure-activity relationships for related analogues. compound 12 (ec50 = 3.7 nm at nk-2 receptors in the rat colon; selectivity > 1000- and > 300-fold with respect to nk-1 and nk-3 receptors, respectively) was derived by incorporation of a gly-leu gamma-lactam conformational constraint into the c-terminal region of the neurokinin a octapeptide analogue [lys3]-nka ... | 1992 | 1331460 |
| transcription of the human ferredoxin gene through a single promoter which contains the 3',5'-cyclic adenosine monophosphate-responsive sequence and sp 1-binding site. | we have investigated the functional elements involved in camp-stimulated transcription of the human ferredoxin gene. unlike the bovine gene, the human gene lacked a second upstream rna initiation site as demonstrated by sequence analysis of the exon boundary, lack of upstream rna, and analysis of the promoter. the presence of a single promoter was determined by testing the ability of various gene segments to drive the expression of the chloramphenicol acetyltransferase gene after transfection in ... | 1992 | 1331772 |
| nuclear bodies in the egg cells of a gyrodactylus species (platyhelminthes, monogenea). | the presence and ultrastructure of distinctive nuclear bodies in a mature 'egg cell' of a gyrodactylus sp. are described. these electron-dense granular bodies, which appear in section as open or closed rings or as a solid mass are not membrane-bound and lie in clusters close to the nucleolus. the nuclear bodies are compared with the nuclear-inclusion bodies previously reported in platyhelminths. the possible origin, significance and role of the nuclear bodies in this gyrodactylus sp. are discuss ... | 1992 | 1332024 |
| anaerobic degradation of 1,2-propanediol by a new desulfovibrio strain and d. alcoholovorans. | a sulfate-reducing bacterium, strain hdv, was isolated from the anoxic soil of a ricefield using lactate as electron donor. cells were gram-negative, motile, nonsporulating curved rods, with single polar flagella. substrates were incompletely oxidized to acetate and included glycerol, 1,2- and 1,3-propanediol. sulfate, sulfite, thiosulfate, elemental sulfur, fumarate, maleate, and malate were utilized as electron acceptors. pyruvate, fumarate, maleate, malate and dihydroxyacetone were fermented. ... | 1992 | 1332638 |
| identification of endogenous 5s,12s-dihydroxy-6,8,10,14-(e,z,e,z)-eicosatetraenoic acid (5s,12s-dihete) in chick osteoblasts and epiphyseal cartilage. | cell homogenates were obtained from chick osteoblast-like cells and epiphyseal growth plates. the lipid fraction was extracted with ethyl acetate and separated by rp-phase hplc. reverse-phase hplc analysis of this fraction showed a major peak displayed a conjugated tetraene structure and this endogenous synthetic compound could be partially blocked by heat treatment at 100 degrees c for 5 min. after methylation, this peak was further purified by sp-hplc and a major peak was identified by an u.v. ... | 1992 | 1333247 |
| prickly pear (opuntia sp.) pectin reverses low density lipoprotein receptor suppression induced by a hypercholesterolemic diet in guinea pigs. | the effects of prickly pear pectin on plasma ldl metabolism were investigated by feeding guinea pigs either a diet containing 15 g/100 g lard and 0.25 g/100 g cholesterol (lc diet) or the lc diet in which cellulose was partially replaced (2.5 g/100 g) by prickly pear pectin (lc-p diet). the lc-p diet lowered plasma ldl cholesterol concentrations by 33% (p < 0.001). low density lipoprotein composition was modified by intake of prickly pear pectin; the relative percentages of free and esterified c ... | 1992 | 1333520 |
| pharmacologic characterization of the novel ligand [4,5-3h-leu9]neurokinin-a binding to nk-2 receptors on hamster urinary bladder membranes. | we synthesized a novel ligand [4,5-3h-leu9]-neurokinin a (3h-nka, s.a 117-144 ci/mmol), and evaluated its binding to hamster urinary bladder membranes (hubm). the ligand bound to hubm in a highly-specific (94 +/- 4%) and protein-dependent manner. binding was rapid (k1 = 0.037 nm-1*min-1) and saturable (bmax = 1210 +/- 177 fmol/mg protein), to a single population of high-affinity sites (kd = 2.41 +/- 0.15 nm, nh = 0.99 +/- 0.02). binding was inhibited by non-hydrolyzable gtp analogs. competition ... | 1992 | 1333574 |
| binding of surfactant protein a (sp-a) to herpes simplex virus type 1-infected cells is mediated by the carbohydrate moiety of sp-a. | pulmonary surfactant protein a (sp-a) has been shown to act as an opsonin in the phagocytosis of viruses by alveolar macrophages. to determine whether sp-a binds to viral proteins and which part of the sp-a molecule is involved in this interaction, binding studies were undertaken. sp-a was labeled with fluorescein isothiocyanate, and its binding to herpes simplex virus type 1-infected hep-2 cells, as a model for virus-infected cells in general, was studied using flow cytometry. the binding of sp ... | 1992 | 1334078 |
| tissue kallikrein-kinin system in the brain and the regulation of arterial pressure. | the intracerebral administration of kininase inhibitors produced an increase in arterial pressure of the shr but not in normotensive animals. the shr were several fold more sensitive to the pressor response elicited by the intracerebral injection of bradykinin, however shr with low blood pressure showed decreased sensitivity to the bradykinin pressor effect. the results suggest that an endogenous kinin system plays a role in the central regulation of blood pressure of the shr. | 1992 | 1334357 |
| use of selective antagonists to dissociate the central cardiovascular and behavioural effects of tachykinins on nk1 and nk2 receptors in the rat. | 1. the effects of intracerebroventricular (i.c.v.) pretreatment with selective nk1 ((+/-)-cp 96,345), nk2a (men 10,207; men 10,376) and nk2b (r 396) tachykinin receptor antagonists on the cardiovascular and behavioural responses to i.c.v. substance p (sp) and neurokinin a (nka) were studied in conscious rats. 2. sp and nka (25 pmol) induced mean arterial blood pressure and heart rate increases of the same magnitude and duration. the cardiovascular responses to both peptides were accompanied by e ... | 1992 | 1335337 |
| [peptidergic innervation of human salivary glands (parotid gland and submandibular gland)]. | immunocytochemistry and a radioimmunoassay were used to investigate the existence and distributions of various regulatory peptide immunoreactivities (ir) in human submandibular and parotid glands. numerous nerve fibers containing vasoactive intestinal polypeptide (vip) and peptide histidine methionine (phm), or neuropeptide tyrosine (npy) and c-flanking peptide of npy (cpon)-ir were found in close proximity to acini, ducts and blood vessels. only a few calcitonin gene-related peptide (cgrp)- and ... | 1992 | 1335445 |
| rabbit surfactant protein c: cdna cloning and regulation of alternatively spliced surfactant protein c mrnas. | surfactant protein c (sp-c), a hydrophobic protein of pulmonary surfactant is essential for surfactant function. toward elucidating molecular mechanisms that mediate regulation of sp-c gene expression in rabbit lung, we isolated and characterized cdnas encoding rabbit sp-c and studied the regulation of sp-c gene expression during fetal lung development and by adenosine 3',5'-cyclic monophosphate (camp) and dexamethasone in fetal lung tissues in vitro. we found that rabbit sp-c is highly homologo ... | 1992 | 1335697 |
| photosensitive nitrile hydratase from rhodococcus sp. n-771. structure and function of the enzyme. | | 1992 | 1335711 |
| an analysis of the effect of changes in growth temperature on proteolysis in vivo in the psychrophilic bacterium vibrio sp. strain ant-300. | in the psychrophilic bacterium vibrio sp. strain ant-300, the rate of protein degradation in vivo, measured at fixed temperatures, increased with elevation of the growth temperature. a shift in growth temperature induced a marked increase in this rate. dialysed cell-free extracts hydrolysed exogenous insulin, globin and casein (in decreasing order of activity) but did not hydrolyse exogenous cytochrome c. cells contained at least seven protease separated by deae-sephacel chromatography, one of w ... | 1992 | 1336030 |
| hormonal influences on morphology and neuropeptide gene expression in the infundibular nucleus of postmenopausal women. | neuronal hypertrophy occurs in a subpopulation of neurons in the infundibular nucleus of post-menopausal women. the hypertrophied neurons contain nkb, sp and estrogen receptor gene transcripts. although associated with reproductive aging, post-menopausal neuronal hypertrophy is not a sign of central nervous system degeneration. quite the opposite, because the hypertrophy is accompanied by marked increases in tachykinin gene expression, reflecting increased neuronal activity. we have proposed tha ... | 1992 | 1336203 |
| [the spontaneous neuronal activity of the hippocampus during the modulation of the theta rhythm by cholinergic substances]. | background activity of the hippocampal neurons, extracellularly recorded in waking chronic rabbits, was analysed in control state and after systemic injection of physostigmine and scopolamine. similar analysis was done in the hippocampus chronically deprived of ascending brain stem afferents. cholinergic drugs controlled the number of hippocampal neurons with theta-modulation and the degree of its stability but not the frequency. activation of cholinergic theta-rhythm resulted also in regulariza ... | 1992 | 1336281 |
| [annual changes of bacteria isolated from urinary tract infections and their susceptibility to new quinolone agents]. | statistical analyses were conducted on bacterial strains isolated from the urine of patients with urinary tract infections (uti) in the department of urology at aichi medical university from january 1988 through december 1991. the drug sensitivity of the bacterial strains isolated from patients with complicated uti between 1988 and 1991 was analyzed and compared. among the outpatients with uncomplicated uti, a total of 352 strains were isolated. e. coli was isolated the most frequently (90.1%). ... | 1992 | 1336639 |
| regional differences of the contractile responses to acetylcholine and neurokinin a in rabbit airway: heterogeneous distribution of the metabolic enzymes. | 1. experiments were designed to determine whether differences exist in the sensitivity to muscarinic and tachykinin agonists in rabbit airways. 2. the rank order of sensitivity (pd2 value) to acetylcholine was: trachea > proximal bronchus > distal bronchus, whereas no regional difference was observed in the sensitivity to carbamylcholine which is resistant to acetylcholinesterase. 3. acetylcholinesterase activity was greater in the distal than in the proximal airway. 4. in the absence of the pep ... | 1992 | 1336745 |
| the processing of beta-endorphin and alpha-melanotrophin in the pars intermedia of xenopus laevis is influenced by background adaptation. | beta-endorphin- and alpha-melanotrophin (alpha-msh)-related peptides were extracted from the pars intermedia of xenopus laevis maintained for 2, 4 or 6 weeks on a white background and for the same periods on a black background. the peptides were resolved under dissociating conditions by gel exclusion chromatography on sephadex g-50 and they were detected by radioimmunoassay with antibodies to beta-endorphin, alpha,n-acetyl beta-endorphin and alpha-msh. the beta-endorphin-related peptides separat ... | 1992 | 1336791 |
| [gastroenteritis caused by adenoviruses 40/41: epidemiological and clinical aspects]. | the 40/41 adenovirus plays an important role as the productive agent of gastroenteritis at a pediatric age according to recent studies. with the aim of evaluating the clinical-epidemiological importance of this agent in our medium a prospective study of the 40/41 adenovirus was carried out in 1,697 stools of children of under 14 years of age over a period of 2 years. an enzyme immunoassay technique with specific monoclonal antibodies was used for detection. the 40/41 adenovirus was the third cau ... | 1992 | 1336979 |
| effects of intrathecal capsaicin and an nk-1 antagonist, cp,96-345, on the thermal hyperalgesia observed following unilateral constriction of the sciatic nerve in the rat. | we evaluated the effect of intrathecal (i.t.) capsaicin (cap) and the nk-1 selective non-peptidic antagonist, cp,96-345, on the thermal hyperalgesia ordinarily observed after unilateral partial ligation of the sciatic nerve in rats. cap was injected i.t. 2 days after constriction injury. seven days after partial ligation, the levels of substance p (sp), calcitonin gene-related peptide (cgrp) and vasoactive intestinal polypeptide (vip) were the same in the left and right dorsal horns of the lesio ... | 1992 | 1337198 |
| [plasmid determined degradation of sulfo-aromatic compounds by pseudomonas sp. bs1304]. | utilized sulfo-aromatic compounds of pseudomonas sp. bs1304 were isolated. it was shown that the first step of conversion is desulfonation with following meta-cleavage of the substituted aromatic ring. at least two steps are controlled by the plasmid pbs1004 (120 kb), belonging to the incp-9 incompatibility group. the degradative plasmid marked by tn5-lac may be mobilized to p. putida, p. aeruginosa, p. mendocina, where a degradative phenotype is expressed. | 1992 | 1337531 |
| [evaluation of a prospective survey on cryptosporidiosis in a population of children hospitalized in strasbourg]. | cryptosporidium sp is a new enteric pathogenic organism which is responsible for self-limited acute diarrhea in infants. during a prospective study we diagnosed the first pediatric cases of cryptosporidiosis in strasbourg. this leads us to recommend that cryptosporidiosis be looked for in the etiological diagnosis of infantile diarrhea. | 1992 | 1337594 |
| comparison of different computational methods for measuring antibodies to avian infectious bronchitis virus in single serum dilution. | twenty-eight one-day-old chickens with infectious bronchitis virus (ibv) maternal antibodies were immunized with strain h120 (bronchovac-i, phylaxia) in spray form. the chickens were kept in an isolator. on day 42 and 56 the chickens were immunized with ibv strain m41 (10(3.0)eid50/0.1 ml). serum antibody titres were measured by both serial dilution and single dilution elisa on day 42, 56 and 76. "twice negative average" (tna), "sample to positive" (sp) and "subtraction method" (sm) titres were ... | 1992 | 1339061 |
| fluoride inhibition of scn- and cl-peroxidase activities in whole saliva and of recombinant myeloperoxidase. influence of ph and hydrogen peroxide concentration. | fluoride (f-) inhibition of peroxidase activity in whole saliva and of recombinant human myeloperoxidase was investigated using thiocyanate (scn-) and chloride (cl-) as substrates. at ph 5.5, scn(-)-linked activity in whole saliva reduced to < 40% of its initial value at f- concentration of 20 mm, while cl- linked activity was maintained at 90% of its initial value for the same f-concentration. based on this cl(-)-linked activity, the contribution of natural mp to the scn(-)-linked activity in w ... | 1992 | 1339090 |
| [gastric emptying and metabolic acidosis. iii. study of gastric retention of a sodium citrate solution using an experimental model of metabolic acidosis in rats]. | the gastric emptying of sodium citrate solution 0.25 meq/ml was studied in rats with metabolic acidosis induced by orogastric infusion of 0.5 m ammonium chloride solution. two control groups were used: one infused with 0.5 m sodium chloride and the other with water. the 3 solutions content was 2 ml/100 g weight of the animal. six hours after the infusion, there was a moderate metabolic acidosis in the group with ammonium citrate. this 6 hour interval marked the beginning of the gastric emptying ... | 1992 | 1339143 |
| purification, characterization and cdna cloning of human lung surfactant protein d. | human pulmonary surfactant protein d (sp-d) was identified in lung lavage by its similarity to rat sp-d in both its molecular mass and its ca(2+)-dependent-binding affinity for maltose [persson, chang & crouch (1990) j. biol. chem. 265, 5755-5760]. for structural studies, human sp-d was isolated from amniotic fluid by affinity chromatography on maltose-sepharose followed by f.p.l.c. on superose 6, which showed it to have a molecular mass of approx. 620 kda in non-dissociating conditions. on sds/ ... | 1992 | 1339284 |
| nucleotide sequence of aminoglycoside 6'-n-acetyltransferase [aac(6')] determinant from serratia sp. 45. | gene for aminoglycoside 6'-n-acetyltransferase [aac(6')] from serratia sp. 45 was cloned into e. coli. the enzyme produced in e. coli carrying the recombinant plasmid was compared to the serratia enzyme. both enzymes acetylated the 6'-c position of amikacin, dibekacin, tobramycin, sisomicin, gentamicin c1a and kanamycin but effected gentamicin c1, gentamicin c2 and micronomycin minimally. no significant difference in optimal ph, isoelectric point or molecular weight was detected. the nucleotide ... | 1992 | 1339319 |
| a region of heterogeneity adjacent to the 5s ribosomal rna gene of cereal rusts. | total genomic dna was isolated from three cereal stem rusts, puccinia graminis f. sp. tritici, f. sp. secalis, f. sp. avenae, and two cereal leaf rusts, p. recondita f. sp. tritici and p. coronata f. sp. avenae, and analyzed for the presence of heterogeneity in the intergenic region of the ribosomal dna repeat unit. a 1 kb region of the repeat unit between the 26s and the 5s rrna genes (igr-1) was amplified by pcr and was found to be heterogeneous within each isolate and variable in size between ... | 1992 | 1339324 |
| nucleotide sequence of the synechococcus sp. pcc7942 heme gene encoding the homologue of mammalian uroporphyrinogen decarboxylase. | we have determined the complete nucleotide sequence of a synechococcus sp. pcc7942 gene encoding the homologue of mammalian uroporphyrinogen decarboxylase (urod). the gene, designated heme, encoded a polypeptide of 354 amino acids with a molecular weight of 39,283. the primary sequences of the polypeptide encoded by heme and human and rat urod had 32.5% identical amino acid residues. no invariant cysteine residues were found, despite the fact that urod isolated from different sources has been sh ... | 1992 | 1339332 |
| identification and sequencing of a gene encoding a hydantoin racemase from the native plasmid of pseudomonas sp. strain ns671. | dna fragments containing the genes involved in the conversion of 5-substituted hydantoins to their corresponding l-amino acids have been cloned from the 172-kb native plasmid (phn671) of pseudomonas sp. strain ns671. the largest recombinant plasmid, designated phpb14, encoded the ability to convert d-5-substituted hydantoins to the corresponding l-amino acids, whereas the smallest one, designated phpb12, encoded the ability to convert them to their corresponding n-carbamyl-d-amino acids. restric ... | 1992 | 1339422 |
| molecular analysis of an esterase-encoding gene from a lipolytic psychrotrophic pseudomonad. | an esterase gene (esta) from a lipolytic psychotroph (pseudomonas sp. ls107d2), was cloned in escherichia coli and its nucleotide sequence was determined, revealing an orf encoding a polypeptide of 389 amino acid residues, with a molecular mass of 42276 da. labelling of plasmid-encoded proteins with [35s]methionine, using the maxicell procedure, gave a single polypeptide of molecular mass 42 kda, consistent with that calculated from the orf. colonies of e. coli cells containing esta produced a c ... | 1992 | 1339464 |
| on some cestodes parasitizing freshwater fish in italy. | the paper presents a systematic survey of some cestodes parasitizing freshwater fish in italy. the following eight species were recorded: monobothrium wageneri, cyathocephalus truncatus, triaenophorus nodulosus (plerocercoids and adults), bothriocephalus acheilognathi, b. claviceps, ligula intestinalis (plerocercoids), schistocephalus sp. (plerocercoids) and proteocephalus percae from perca fluviatilis which is reported from freshwater fish in italy for the first time. all the tapeworms recovere ... | 1992 | 1339972 |
| [laboratory diagnosis and symptoms of dengue, during an outbreak in the ribeirão preto region, sp, brazil]. | a dengue type 1 outbreak started in the ribeirao preto region, north of sao paulo state, brazil, in november of 1990. about 3500 dengue cases were confirmed by blood tests until february of 1991. the virus research unit of the faculty of medicine of ribeirao preto-sao paulo state university, studied 502 dengue suspect cases. the serologic diagnosis of dengue type 1 was confirmed by haemagglutination inhibition test (hai) in 19% of the cases. diagnosis was done later by using an enzyme immuno ass ... | 1992 | 1340025 |
| [non-cholera vibrios in enterobacteriologic routine]. | of 3250 diarrheal stools received for microbiologic diagnosis at a private clinical laboratory in recife, brazil, strains of vibrio were isolated from 55 (1.7%). the study was carried out from may 1989 through may 1991. for recovering vibrio, fecal samples were enriched in alkaline peptone water supplemented with 2% nacl and subcultured on thiosulfate-citrate-bile salts-sucrose agar (tcbs). of the recovered species, v. parahaemolyticus was most commonly found (24 strains), followed by v. furniss ... | 1992 | 1340026 |
| benthic dinoflagellates from the coral reef lagoon of mayotte island (s-w indian ocean); identification, toxicity and preliminary ecophysiological study. | the community of benthic dinoflagellates of mayotte island is similar to those of other regions. four clones of gambierdiscus toxicus and of other benthic dinoflagellates species (prorocentrum spp., ostreopsis sp., amphidinium spp.) have been isolated and screened for their crude toxicity using mouse-test. the toxigenic reservoir seems moderated. one toxic clone of g. toxicus has been studied for factors governing growth and photosynthetic activity of this species: salinity, temperature, light i ... | 1992 | 1340343 |
| [liver disease induced by acetaminophen: a model of hepatotoxicity]. | | 1992 | 1340366 |
| [effect of tamoxifen on the bone mass]. | | 1992 | 1340384 |
| [isolation of oocysts of cryptosporidium sp. in loose stools of patients with aids and of immunocompetent children and adults in são paulo, brazil]. | on the aim of knowing better the prevalence of cryptosporidiosis in brazil, we performed a survey, in são paulo, in loose stools of patients with aids and immunocompetent children and adults. using strict methods oocysts of cryptosporidium sp. were found in 10.4% of the samples; higher rates were observed for patients with aids, and children attending day-care centers. the result observed may be considered important and indicate the need to include the search of this organism in routine stool ex ... | 1992 | 1340591 |
| [the evaluation of the effect of formol in the detection of cryptosporidium sp. oocysts in feces by the methods of sheater and of the coprotest]. | frequently it is necessary to search for oocysts of cryptosporidium sp. in the stools potentially contaminated with human immunodeficiency virus (hiv). for the protection of the laboratory personal handling fecal material formalin has been considered useful. however, this disinfectant has not been evaluated when the stool examinations were performed with the technique of flotation-centrifugation in concentrated solution of sugar and with that of "coprotest". our experience indicates that the use ... | 1992 | 1340607 |
| [identification and sensitivity of acinetobacter sp isolated from clinical specimens and hospital environment]. | one hundred thirty two strains of acinetobacter isolated between october 1989 and march 1991 at the san juan de dios hospital, santiago de chile were included in this study. one hundred twelve isolates were obtained from patients and 20 from the hospital environment. among the 112 clinical isolates, 108 (96.4%) were identified according to the new classification proposed by bouvet and grimont in 1986 as a. baumannii, and four as acinetobacter genospecies 3. the 20 strains obtained from the hospi ... | 1992 | 1340947 |
| study of interactions between listeria monocytogenes and experimental tumors. | to verify the effects of listeria monocytogenes (lm) inoculation in the survival of animals bearing ehrlich's tumor. kind of study: experimental. animals-isogenic mice, balb/c, female, 19-21 g. tumor-ascitic ehrlich's tumor, dilution of 5 x 10(5) cells/0.1 ml. bacteria-lm serotype 4a, solution with 7 x 10(3) bacteria (standard sub-lethal dose). intervention-a) inoculation of lm in mice bearing ehrlich tumor at the same time as ascitic cells transplantation. b) inoculation of lm seven days before ... | 1992 | 1340994 |
| latero-terminal neurorrhaphy without removal of the epineural sheath. experimental study in rats. | termino-lateral neurorrhaphies have been used up to the beginning of this century. after this period, they have no longer been reported. we tested the efficacy of a new type of latero-terminal neurorrhaphy and evaluated the role of the epineural sheath. a group of 10 rats had the fibular nerve sectioned and the distal ending was sutured to the lateral face of the tibial nerve without removing the epineurium. all experiments were made on the right side, the left one remaining untouched in half of ... | 1992 | 1341024 |
| decalcification of mineralized rat mandible tissues with the aid of microwaves. | | 1992 | 1341027 |
| [the cholesterol profile in the city of são josé do rio preto]. | to analyze: a) the cholesterol profile in adults older than 29 years, from são josé do rio preto (300,000 inhabitants), city located in one of the country's richest regions; b) the variables that correlated with cholesterolemia. | 1992 | 1341866 |
| effects of cyclosporin a immunosuppression on pancreaticoduodenal transplantation in alloxan-diabetic rats. | 1. forty-five outbred wistar rats were randomly assigned to three experimental groups: gi, 10 non-diabetic control rats; gii, 10 alloxan-diabetic control rats; giii, 25 alloxan-diabetic rats which received pancreaticoduodenal transplantation (pdt) from normal wistar donor rats and were immunosuppressed with cyclosporin a (cy-a), 10 mg kg body weight-1 day-1, administered intraperitoneally for 30 days. 2. in parallel, 15 alloxan-diabetic inbred wistar rats received isogeneic pdt from normal wista ... | 1992 | 1341913 |
| inhibition of insulin release by jatrophone. | in many countries, including brazil, extracts of jatrophona elliptica species are currently used for the treatment of several diseases. recently it was shown that a purified compound from these plants inhibits contraction of smooth and cardiac muscle in the microm range, probably involving alterations in membrane ca2+ permeability and/or internal ca2+ distribution. in collagenase-isolated rat islets and in the absence of glucose, basal insulin secretion measured by radioimmunoassay averaged 122 ... | 1992 | 1341926 |
| mobilization of nonconjugative pbr322-derivative plasmids from laboratory strains of escherichia coli to bacteria isolated from seawater. | mobilization of derivatives of plasmids pbr322 and pbr325 was shown to occur between escherichia coli k12 strains in lb-broth at 37 degrees c, provided a mobilizer plasmid (f') was present either together with the nonconjugative plasmid or in a second donor strain. evidence from restriction endonuclease analysis suggested that the mobilization was facilitated by a transposition phenomenon involving the "gamma-delta" sequence of f'. it was shown that mobilization of a derivative of pbr325 from e. ... | 1992 | 1341984 |
| behavior in agricultural soils of a recombinant pseudomonas bacterium that simultaneously degrades alkyl- and haloaromatics. | pseudomonas sp. fr1 (pfrc20p) is a recombinant bacterium able simultaneously to degrade alkyl- and haloaromatics due to the xylxyzl and xyls genes from the tol plasmid borne on the bacterial chromosome, and to the gene encoding for a gamma-lactone isomerase from alcaligenes eutrophus on pfrc20p. the survival of this strain in sterile soils was shown to depend on the physicochemical properties of the soil. the recombinant information was stable in bacteria introduced in soils and conferred select ... | 1992 | 1341985 |
| antinociception produced by 2 and 5 khz peripheral stimulation in the rat. | recently a "medium frequency" electric stimulator (likon) was marketed, which delivers 2000-5000 hz square waves and was claimed to induce a strong clinical analgesic effect. the aim of this study was to clarify whether electric stimulation over 1000 hz would induce any antinociceptive effect in the rat. tail flick latency (tfl) was taken as the nociceptive index. electrical stimulation was applied via stainless steel pins inserted into the acupoint st 36 located near the knee joint and sp 6 loc ... | 1992 | 1342034 |
| [cryptosporidium parasitism in children with acute diarrhea]. | during the period from august 1987 to july 1990, 241 fecal samples collected from 1 to 48 months old children with acute diarrhea and examined in the child's institute of hospital das clínicas of faculdade de medicina da universidade de são paulo, were submitted to parasitological tests in the enteroparasitosis section of the instituto adolfo lutz. fourty two (17.43%) fecal samples showed cryptosporidium sp. oocysts by carbol-fucsin stain. parasitism by cryptosporidium sp. was more frequent duri ... | 1992 | 1342092 |
| listeriosis and aids: case report and literature review. | listeriosis is a not uncommon infection in humans, usually associated with immunodeficient states and with newborns. however, relatively few cases have been reported in hiv-infected patients. this scarcity of reported cases has aroused interest in the association of listeriosis and aids. in this paper we present a case of meningitis and septicemia caused by listeria monocytogenes in a female patient with aids. a review of recent medical literature indicates that association of listeriosis and ai ... | 1992 | 1342113 |
| influence of diabetes mellitus on the glutathione redox system of human red blood cells. | several components of the erythrocyte-dependent glutathione redox system (reduced glutathione, gsh; oxidized glutathione, gssg; glutathione peroxidase, gsh-px; glutathione reductase, gsh-red) were determined in patients with types i and ii diabetes mellitus (dm). all groups studied were male subjects: g1, 20 young healthy individuals (aged 23.7 +/- 4.2 years); g2, 15 young insulin-treated type i dm patients; g3, 20 older insulin-treated type ii dm patients; g4, 21 older oral hypoglycemic agent-t ... | 1992 | 1342208 |
| the acute phase of experimental infection with trypanosoma cruzi is more severe in mice monoassociated with strict anaerobic bacteria. | 1. the influence of some components of the normal human intestinal flora on the acute phase of experimental infection with strain cl of trypanosoma cruzi was studied in 30-day-old germ-free or gnotobiotic cfw (lob) mice monoassociated with bacteroides fragilis, peptostreptococcus sp or clostridium sp by intragastric inoculation of 10(6) bacteria 10 days before the intraperitoneal infection with 5 x 10(3) trypomastigotes/g body weight. 2. significantly earlier parasitemia peak and mortality were ... | 1992 | 1342224 |
| type i collagen synthesis by rats fed beans (phaseolus vulgaris, l.) as the protein source. | type i collagen synthesis was studied in 12 female wistar rats weighing 60 +/- 5 g at the beginning of the experiment. the animals were fasted for 24 h and then injected ip with 10 microci uniformly labeled [14c]-glycine. two hours later, groups of 4 animals each were fed balanced diets (10.7 +/- 0.4% protein) containing raw beans (phaseolus vulgaris, l.), cooked beans or casein (control) as the single protein source, ad libitum. the animals were killed after 4 days and collagen was extracted fr ... | 1992 | 1342225 |
| comparison of indirect immunofluorescence with agar gel immunodiffusion for the diagnosis of bluetongue virus infection. | 1. sera from 190 cows and from 72 sheep were examined to compare the results obtained with the agar gel immunodiffusion (agid) and indirect immunofluorescence (iif) tests for the diagnosis of bluetongue (bt) disease. 2. in the agid test, 96 of 190 (50.5%) cattle serum samples and 38 of 72 (52.7%) sheep serum samples were positive, for a total of 134 out of 262 (51.1%) sera. in the iif test, 98 of 190 (51.6%) cattle serum samples and 39 of 72 (54.2%) sheep serum samples were positive, for a total ... | 1992 | 1342226 |
| effect of exercise during pregnancy and dam age on maternal blood chemistry and fetal growth. | in order to determine the effect of maternal exercise on maternal nutritional status and fetal growth, young (y = 45-50 days old) wistar rats were divided into 4 groups of 5 to 8 animals: control pregnant (cp), control non-pregnant (cnp), exercise-trained (swimming 1 h/day, 5 days/week, for 19 days) pregnant (tp) and exercise-trained non-pregnant (tnp). four equivalent groups of adult rats (a = 90-100 days old) were also formed. serum glucose, total protein, albumin, hematocrit and liver glycoge ... | 1992 | 1342230 |
| interactions between vasodilator drugs and human hemoglobin. | 1. the in vitro effect of the vasodilators papaverine, dipyridamole, nifedipine, prenylamine and glyceryl di-, tri- and tetranitrate on the oxygen saturation curve of human hemoglobin (hb) was evaluated. the hb dissociation curve was measured spectrophotometrically both for freshly obtained heparinized blood and for purified hb a after incubation with the drugs. 2. for both purified hb and whole blood samples, incubation with papaverine, dipyridamole and nifedipine modified p50 (po2 for half sat ... | 1992 | 1342232 |
| an allergenic 2s storage protein from ricinus communis seeds which is a part of the 2s albumin precursor predicted by c-dna data. | 1. a 1.9s albumin having allergenic activity and denoted ric c iii was isolated from an alcohol extract of defatted ricinus communis seeds, cb-1a, as a homogeneous protein by ion-exchange chromatography on sp-sephadex, gel filtration on sephadex g-75 and preparative polyacrylamide gel electrophoresis (6 mg ric c iii/g cb-1a). 2. the protein contained approximately 98 amino acid residues distributed in 2 chains of 67 and 34 residues, a molecular weight of 11,239 based on amino acid composition an ... | 1992 | 1342233 |
| [characterization and antigenic relationship of 3 new bunyaviruses in the group anopheles a (bunyaviridae) of arboviruses]. | the isolation and characterization of three new viruses obtained from the tucuruí hydroelectric dam region is reported. these three agents belong to the anopheles a serogroup, genus bunyavirus, bunyaviridae. the tucuruí (tuc), caraipé (cpe) and arumateua (art) viruses have close relationships with each other and with trombetas (tbt) virus, an anopheles a virus previously isolated in the amazon region of brazil. these viruses form the "trombetas complex". tuc, cpe and art viruses were obtained fr ... | 1992 | 1342498 |
| [presence of intestinal parasites in vegetables sold in the metropolitan region of são paulo, sp, brazil. i--search of helminths]. | vegetables in natura, commercially traded in the metropolitan area of s. paulo, were analysed by the appropriate methodology with a view to discovering an identifying the paths of transmission of enteroparasites of medical interest. the vegetables under study consisted of 50 samples of each variety listed below: lettuce (lactuca sativa)-oily leaves and crisphead varieties, endive (chicorium sp) and water-cress (nasturtium officinale). results showed high rates of contamination in all the vegetab ... | 1992 | 1342513 |
| [presence of intestinal parasites in vegetables sold in the metropolitan area of são paulo-sp, brazil. ii--research on intestinal protozoans]. | vegetables in nature, commercially traded in the metropolitan area of s. paulo, brazil, were analyzed by means of the appropriate methodology with a view to discovering and identifying protozoan cysts of medical interest. the vegetables under study consisted of 50 samples of each of the varieties listed below: lettuce (lactuca sativa)-oily leaves and crisp-head varieties, endive (chicorium sp) and watercress (nasturtium officinale). results showed high rates of contamination in all the varieties ... | 1992 | 1342522 |
| gangliosides inhibit serological reactions for the detection of cholera and heat-labile enterotoxins of escherichia coli. | gm1 ganglioside has been identified as the receptor for cholera toxin (ct) and heat-labile (lt) enterotoxin of escherichia coli in many cell types. using the radial immune hemolysis (rih) and indirect hemagglutination (ih) tests described for the detection of these enterotoxins, a study was conducted on the 100% inhibition of these reactions by pre-incubating these enterotoxins with gm1, gd1a and gt1 gangliosides. gm1 was found to be much more efficient than the other two. with respect to the ri ... | 1992 | 1342612 |
| further studies on the hypoxia produced by canatoxin in rats. | canatoxin, a convulsant neurotoxin from the seeds of canavalia ensiformis, induces lipoxygenase-dependent hypoxia in rats which is blocked by hexamethonium. the purpose of the present study was to examine the relationship between canatoxin-induced hypoxia and bronchoconstriction. since several effects of the toxin are very similar to those described for morphine and opioid-like peptides, the effects of opioid antagonists were also investigated. pretreatment of male, adult wistar rats (200-250 g) ... | 1992 | 1342621 |
| lesions of the lateral hypothalamus impair the pressor response to clonidine injected into the medial septal area of conscious rats. | the central injection of clonidine (an alpha 2-adrenoceptor agonist) in conscious normotensive rats produces hypertensive responses and bradycardia. the present study was performed to investigate the effect of electrolytic lesions of the lateral hypothalamus (lh) on the pressor and bradycardic responses induced by clonidine injected into the medial septal area (msa) in conscious and unrestrained rats. male holtzman rats weighing 250-300 g were used. mean arterial pressure and heart rate were rec ... | 1992 | 1342623 |
| effect of moisture content on the microbial activity in jp-5 fuel oil. | the water solubility of jp-5 fuel oil was found to be proportional to the reciprocal of absolute temperature from 0 degree c to 60 degrees c. water in the fuel oil would become condensed once the temperature was shifted from a high temperature to low temperature. during the storage, condensed water was precipitated in the bottom of the tank. both the static and the dynamic dehumidification processes with molecular sieve could reduce the moisture content of fuel oil to less than 5 ppm. however, p ... | 1992 | 1342639 |
| in vitro action of vasopressin on water absorption by rat colon. | the action of vasopressin on water absorption was studied in vitro in the colon of rats, weighing approximately 250 g. everted sacs of intestinal segments (ascending and descending colons) prepared with mucosa only were used. vasopressin (10 mm) significantly stimulated water absorption in both the proximal (4.85 +/- 3.78 vs 1.51 +/- 1.16 ml/mg) and distal (10.39 +/- 3.52 vs 7.22 +/- 3.58 ml/mg) colon, which corresponds to an increase of 220% and 50%, respectively. the results indicate the need ... | 1992 | 1342827 |