| parvovirus b19 and autoimmune diseases. | parvovirus b19 (b19) causes many clinical disorders, of which the most common are erythema infectiosum, aplastic crisis complicating chronic hemolytic anemia, and hydrops fetalis. in young adults, the skin eruption caused by b19 is accompanied with polyarthritis and polyarthralgia in 60% of the cases. the joint abnormalities predominate in the hands and feet and usually resolve within a week (range 2-21 d). serological tests show igm antibodies against b19, confirming the diagnosis of recent inf ... | 2003 | 12639611 |
| nephrotic syndrome associated with human parvovirus b19 infection. | a previously healthy 8-year-old japanese boy developed nephrotic syndrome during the course of erythema infectiosum due to human parvovirus b19 (pvb19) infection. a renal biopsy showed mesangiocapillary proliferative glomerulonephritis with immune complex deposits associated with pvb19 virus. his renal involvement improved spontaneously. | 2003 | 12644924 |
| gloves and socks lymphangitis associated with acute parvovirus b19 infection. | | 2003 | 12657026 |
| case of stroke in a 7-year-old male after parvovirus b19 infection. | a 7-year-old male presented sudden-onset left hemiparesis, left-sided paresthesia, central paralysis of the left vii cranial nerve, and subsequent headache. magnetic resonance scans were obtained 24 hours after admission. t(2)-weighted images disclosed hyperintensities located mainly in the posterior portion of the lenticular nucleus and in the head and body of the right caudate nucleus. a diagnosis of ischaemic stroke was made on the basis of neuroradiologic findings. laboratory tests undertake ... | 2003 | 12657425 |
| differences in the immunological responses in drug- and virus-induced cutaneous reactions in children. | the cutaneous symptoms in non-immediate reactions to drugs are not always clinically distinguishable from those induced by viruses, especially during the early phase of the reaction. moreover, viral infections and drug reactions often coexist and identification of the etiological agent is necessary. discerning the differences in the immunological response between both may help in the diagnosis. the aim of this study was to determine possible differences in the immunological response in non-immed ... | 2003 | 12667995 |
| heat sensitivity of human parvovirus b19. | to date there has been no published report on a systematic evaluation of the heat sensitivity of human parvovirus b19 (b19) and the related safety of the plasma-derived fractionated products. in this study, we examined the heat sensitivity of b19 by using the infectivity assay with cultured cells. | 2003 | 12670364 |
| two-year follow-up study after human parvovirus b19 infection. | acute human parvovirus b19 (b19) infection induces systemic lupus erythematosus (sle)-like symptoms. it has been controversial whether b19 infection causes sle and rheumatoid arthritis (ra). | 2003 | 12673074 |
| amniotic band syndrome with significant orofacial clefts and disruptions and distortions of craniofacial structures. | amniotic band syndrome (abs) is a rare disorder in which bands of mesoderm that emanate from the chorionic side of the amnion and insert on the fetal body can generate a wide variety of disfiguring and disabling malformations. it usually is sporadic, and the incidence is approximately 1 in 15,000 live births, and affected children typically require involvement of several pediatric surgical subspecialties. the authors describe a case of abs with extensive craniofacial anomalies. | 2003 | 12677585 |
| parvovirus b19 infection presenting with necrotizing lymphadenitis. | | 2003 | 12681470 |
| collapsing glomerulopathy. | collapsing glomerulopathy is a morphologic variant of focal segmental glomerulosclerosis (fsgs) characterized by segmental and global collapse of the glomerular capillaries, marked hypertrophy and hyperplasia of podocytes, and severe tubulointerstitial disease. the cause of this disorder is unknown, but nearly identical pathologic findings are present in idiopathic collapsing glomerulopathy and human immunodeficiency virus (hiv)-associated nephropathy, and collapsing glomerulopathy has been asso ... | 2003 | 12704581 |
| successful treatment of chronic parvovirus b19 infection by high-dose immunoglobulin. | | 2003 | 12708574 |
| lack of association between b19 or v9 erythrovirus infection and anca-positive vasculitides: a case-control study. | to examine the potential association of human b19 or v9 erythrovirus infection and onset of anca-positive vasculitides. | 2003 | 12709542 |
| the role of microvascular injury in the evolution of idiopathic pulmonary fibrosis. | interstitial lung disease compatible with idiopathic pulmonary fibrosis (ipf) developed in 19 previously healthy patients. although interstitial and/or honeycomb parenchymal fibrosis was present in all, there were patchy areas of paucicellular septal capillary injury along with corroborative direct immunofluorescent evidence of a humorally mediated microvascular injury syndrome. significantly elevated factor viii levels were seen in 17 of 18 patients tested. antiphospholipids were present in all ... | 2003 | 12710128 |
| parvovirus b19 encephalitis presenting as immune restoration disease after highly active antiretroviral therapy for human immunodeficiency virus infection. | illness occurring during the initial months of highly active antiretroviral therapy (haart) for human immunodeficiency virus infection may be a consequence of the restoration of an immune response against opportunistic pathogens (i.e., immune restoration disease [ird]). we describe a young man who had aids complicated by parvovirus b19 infection and rbc aplasia and who developed a painless, progressive dyspraxia of the left arm and an expressive dysphasia 4 weeks after commencing effective haart ... | 2003 | 12715316 |
| successful intravenous immunoglobulin therapy in 3 cases of parvovirus b19-associated chronic fatigue syndrome. | three cases of chronic fatigue syndrome (cfs) that followed acute parvovirus b19 infection were treated with a 5-day course of intravenous immunoglobulin (ivig; 400 mg/kg per day), the only specific treatment for parvovirus b19 infection. we examined the influence of ivig treatment on the production of cytokines and chemokines in individuals with cfs due to parvovirus b19. ivig therapy led to clearance of parvovirus b19 viremia, resolution of symptoms, and improvement in physical and functional ... | 2003 | 12715326 |
| [human parvovirus b19]. | | 2003 | 12718036 |
| prevalence of parvovirus b19 in liver tissue: no association with fulminant hepatitis or hepatitis-associated aplastic anemia. | parvovirus b19 has been proposed as the etiological agent of fulminant hepatitis (fh) or hepatitis-associated aplastic anemia (haa). we studied the prevalence of parvovirus b19 in liver-tissue samples from patients with fh and haa and from control subjects. in the first study, parvovirus b19 dna was detected by nested polymerase chain reaction (pcr) in 4 of 15 livers from patients with fh and in 3 of 22 livers from patients with nonviral hepatic disease. in a second confirmatory study, livers we ... | 2003 | 12721938 |
| [erythema infectiosum]. | | 2003 | 12722210 |
| plasmodium falciparum malaria with coexisting parvovirus b19 infection. | | 2003 | 12736416 |
| neurological manifestations of human parvovirus b19 infection. | since its discovery, human parvovirus b19 has been linked with a broad spectrum of clinical syndromes. an aetiological role for the virus has been confirmed in erythema infectiosum, transient aplastic crisis, persistent infection manifesting as pure red cell aplasia in immunocompromised persons, non-immune hydrops fetalis and arthritis. less commonly recognised, but receiving increasing attention recently, are the neurological manifestations, a variety of which have been described in patients wi ... | 2003 | 12740833 |
| significant decrease in hemoglobin and hematocrit levels in a virologically controlled hiv-infected patient. pure red cell aplasia (prca) caused by human parvovirus b19. | | 2003 | 12741369 |
| acute severe anaemia in an elderly patient with hereditary sphaerocytosis. | | 2003 | 12743353 |
| [post-infectious glomerulonephritis]. | post-infectious glomerulonephrites (gns) include a wide spectrum of nephropathies, with known etiological agent, bacterial, parasitic, viral. among gns secondary to bacterial infections, post-streptococcal gn is the most frequent; nevertheless, its incidence in developed countries has decreased during the last 20 years, while some of the characteristics such as types of infection, exposed subjects, clinical and evolutionary patterns have changed. prognosis has worsened and is correlated with som ... | 2003 | 12746805 |
| prevalence of sindbis-related (pogosta) virus infections in patients with arthritis. | to determine the role of pogosta virus as a triggering infection in non-specific arthritis. | 2003 | 12747277 |
| prevalence of human erythrovirus b19 dna in healthy belgian blood donors and correlation with specific antibodies against structural and non-structural viral proteins. | human parvovirus (erythrovirus) b19 is recognized as a major contaminant of blood and blood products. to reduce the risk of contamination, plasma-pool screening and exclusion of highly viraemic donations are recommended. the objectives of this study were to estimate the prevalence of b19 dna in our blood-donor population, to determine the appropriate pool size to be tested (taking into account parameters such as prevalence, viral load, test sensitivity, and the efficacy of inactivation procedure ... | 2003 | 12757504 |
| subclinical parvovirus b19 infection in children with sickle cell anemia. | to investigate the prevalence and clinical consequences of previous parvovirus b19 exposure in a large cohort of pediatric patients with sickle cell anemia (sca). | 2003 | 12759625 |
| [multiple pseudo-cellulitis plaques and koplick's sign: a particular form of parvovirus b19 primo-infection in adults]. | clinical presentation which seems to be more specific of this infection. | 2003 | 12763177 |
| ex vivo cytokine responses against parvovirus b19 antigens in previously infected pregnant women. | parvovirus b19 infection is a significant cause of fetal death. the aim of this study was to investigate the role of maternal immune status in modulating susceptibility to fetal b19 infection. peripheral blood was obtained from pregnant women (n = 199) with no clinical evidence of recent b19 infection. evaluation of ex vivo t cell responses from 149/199 individuals showed significantly higher interferon-gamma levels for seropositive individuals following vp1 (268 +/- 36 versus 103 +/- 19 pg/ml; ... | 2003 | 12767014 |
| clinical utility of the irf: assessment of erythroid regeneration following parvo b19 infection. | parvo b19 (fifth disease) is an erythrotropic virus which attaches through the 'p' globoside receptor on the surface of human red blood cells and precursors. this typically benign viral infection can cause a transient aplastic anemia in patients with underlying red cell disorders. in this case, a two-year-old child presents with severe aplastic anemia without evidence of underlying disease. erythroid regeneration is monitored through the use of the immature reticulocyte fraction (irf) and is dem ... | 2002 | 12776780 |
| risk of parvovirus infection by immunoglobulin. | | 2003 | 12786811 |
| drug-induced hypersensitivity syndrome associated with epstein-barr virus infection. | association of drug-induced hypersensitivity syndrome with viral infection is debated. human herpesvirus 6 (hhv-6) reactivation has been the most frequently reported infection associated with this syndrome. however, a case of cytomegalovirus (cmv) infection was recently described associated with anticonvulsant-induced hypersensitivity syndrome. we report a case of severe allopurinol-induced hypersensitivity syndrome with pancreatitis associated with epstein-barr virus (ebv) infection. active ebv ... | 2003 | 12786838 |
| papular-purpuric "gloves and socks" syndrome in a mother and daughter. | the papular-purpuric "gloves and socks" syndrome (ppgss) is a unique exanthem characterized by petechiae with painful edema of the hands and feet extending proximally with less severity. constitutional symptoms of fever, lethargy, and arthralgia have also been described. human parvovirus b19 has been implicated in most cases as the causative agent. we describe a mother and her daughter presenting with the characteristic findings of ppgss and demonstrating the seroconversion of human parvovirus b ... | 2003 | 12789189 |
| prevalence of the parvovirus b19 genome in endomyocardial biopsy specimens. | although enteroviruses have long been considered the most common cause of inflammatory heart muscle diseases, parvovirus b19 (pvb19) is emerging as a new and important candidate for myocarditis and dilated cardiomyopathy with inflammation (dcmi) and without inflammation (dcm). we investigated left ventricular endomyocardial biopsy specimens from 110 patients with suspected inflammatory heart disease for the presence of pvb19, coxsackie virus (cvb), and adenovirus (ad2) genome by polymerase chain ... | 2003 | 12792925 |
| systemic lupus erythematosus and parvovirus b-19: casual coincidence or causative culprit? | systemic lupus erythmatosus (sle) is a multi-system autoimmune disease characterized by auto-reactive cells and auto-antibodies, which can potentially affect all organ systems. typical organ systems that are affected include the heart, lungs, skin, kidneys, and central nervous system. its expression is believed to be dependent on various factors such as genetic predisposition, environmental agents, immune dysregulation, crossreactivity with auto-antigens, alterations in auto-antigens, or most li ... | 2003 | 12794260 |
| frequent infection with a viral pathogen, parvovirus b19, in rheumatic diseases of childhood. | to find further evidence of the association of parvovirus b19 infection with juvenile rheumatic diseases, and to get new insights into the immunopathogenesis of these diseases. | 2003 | 12794831 |
| clinical presentation of parvovirus b19 infection in hiv-infected patients with and without aids. | human parvovirus b19 replicates in erythrocyte precursors. usually, there are no apparent hematological manifestations. however, in individuals with high erythrocyte turnover, as in patients with sickle-cell disease and in the fetus, the infection may lead to severe transient aplasia and hydrops fetalis, respectively. in aids patients, persistent infection may result in chronic anemia. by contrast, in hiv-positive patients without aids the infection evolves as a mild exanthematous disease. two c ... | 2003 | 12806467 |
| screening for infectious diseases. | introduction: fetal brain injury is an essential cause of lifelong morbidity. infection appears as a cause of brain damage. apart from chorioamnionitis, screening for infectious diseases must be considered in pregnancies with a risk of congenital infection or cases with abnormal cerebral ultrasound findings. discussion: congenital infections include most of the major components of the torch complex: toxoplasmosis, rubella, cytomegalovirus, herpes, and varicella. seronegative mothers can develop ... | 2003 | 12811485 |
| aetiology in sixteen cases of toxic epidermal necrolysis and stevens-johnson syndrome admitted within eight months in a teaching hospital. | toxic epidermal necrolysis and stevens-johnson syndrome are serious cutaneous reactions associated with significant mortality and morbidity. eight patients with toxic epidermal necrolysis and eight patients with stevens-johnson syndrome were admitted consecutively to a single centre between august 2001 and march 2002. an aetiological study including viral serology and pcr was performed in view of the clustering of admissions related to these two conditions. the majority of cases were drug induce ... | 2003 | 12816151 |
| establishment of a porcine parvovirus (ppv) dna standard and evaluation of a new lightcycler nested-pcr assay for detection of ppv. | porcine parvovirus (ppv) is a major causative agent in a syndrome of reproductive failure in swine. in validation (viral clearance) studies, ppv is a model of non-enveloped viruses and is widely used instead of human parvovirus b19, which causes a variety of illnesses including erythema infectiosum (fifth disease) in children and hydrops fetalis in pregnant women. to improve the sensitivity of current pcr-based assays for detection of ppv and to standardize the quantification of ppv, we have dev ... | 2003 | 12821192 |
| [diagnosis of parvovirus b19 infection in patients with secondary immunodeficiency diseases]. | the method of immune-enzyme assay was used to examine 113 patients with secondary immunodeficiency, including 16 hiv-infected drug-addicts (group 1), 36 patients with cytomegalovirus infection (cmvi) and with immunoregating index cd4/cd8 below 1.0 (group 2), 30 patients with cmvi and with cd4/cd8 below 1.2 (group 3) and 31 patients with aplastic anemia and with anemia of unclear genesis (group 4), for parvovirus infection caused by parvovirus b19. as for groups 1 and 4, the antibodies were detec ... | 2003 | 12822304 |
| viral causes of cardiac inflammation. | over the past year there have been few significant breakthroughs in the understanding of the etiologies of viral myocarditis or dilated cardiomyopathy (dcm). one interesting trend has been the increasing number of reports of myocarditis associated with parvovirus b19 infection. whether this is simply a result of improved diagnostics, or reflects an underlying change in the etiology is unclear. however, studies of the underlying mechanisms of these disorders have resulted in several reports linki ... | 2003 | 12826818 |
| [molecular diagnosis of viral materno-fetal infections]. | the main viral infections prenatally detected in fetuses are: cytomegalovirus (cmv), parvovirus b19, rubella virus and varicella-zoster virus infections. prenatal diagnosis is based on the direct detection of the virus by culture (cmv), of its antigens (cmv) or of its genome, essentially by pcr. this direct detection can be done either on fetal blood or on amniotic fluid. prenatal diagnosis can also be performed by detection of specific igm in fetal blood (rubella). non specific markers of viral ... | 2003 | 12843959 |
| antiphospholipid antibodies in pediatric and adult patients with rheumatic disease are associated with parvovirus b19 infection. | to show a possible association between parvovirus b19 infection and the presence of antiphospholipid antibodies (apl) in patients with rheumatic diseases. | 2003 | 12847688 |
| testing for hematologic disorders and complications. | this review summarizes state-of-the-art and emerging techniques in the antenatal diagnosis of fetal anemia and hemoglobinopathies. fetal anemia may result from hemolytic disease, hemorrhage, suppression of erythropoiesis, infection (eg, parvovirus b19), or trauma. the clinical laboratory plays an essential role in the evaluation of these disorders by way of the use of various hematologic, biochemical, serologic, cytometric, and molecular genetics methods. hemoglobinopathies are the most common c ... | 2003 | 12848447 |
| intravenous immunoglobulins in infectious diseases: where do we stand? | intravenous immunoglobulins (ivig) are therapeutic preparations of normal human igg that have been used for more than 20 years for substitutive therapy in patients with primary antibody deficiencies. recent studies pointed out the need to obtain normal residual levels of igg (i.e. 8 g/l) in order to reduce the number and severity of bacterial infections in these patients. the ivig are also prescribed for the substitutive therapy of secondary immunodeficiencies such as chronic lymphoid leukemia a ... | 2003 | 12848745 |
| parvovirus b19 infection and autoimmune disease. | human parvovirus b19 infections may cause a widespread benign and self-limiting disease in children and adults, known as erythema infectiosum or fifth disease. a variety of further manifestations are associated with the infection such as arthralgias, arthritis, leukopenia and thrombocytopenia, anemia and vasculitis, spontaneous abortion and hydrops fetalis in pregnant women. both in children and adults parvovirus b19 infections have been frequently implicated as a cause or trigger of various for ... | 2003 | 12848949 |
| papular-purpuric "gloves and socks" syndrome caused by parvovirus b19. | this paper presents a 15-year-old boy with an acute febrile illness accompanied by purpuric and papular lesions located mostly on the dorsal areas of his hands and feet with the additional changes on his knees and elbows. serologic studies confirmed the acute infection by parvovirus b19. apart from mild leukocytosis there were no other abnormalities in hematologic and laboratory findings. the diagnosis of papular-purpuric "gloves and socks" syndrome (ppgss) was made. cutaneous changes completely ... | 2003 | 12852167 |
| human parvovirus b19 infection during the inactive stage of systemic lupus erythematosus. | a 42-year-old woman with systemic lupus erythematosus (sle) had an episode of fever, arthralgia and anemia. in order to treat the suspected activation of sle, the daily dose of steroid was increased, however, the anemia progressed and pancytopenia developed. both igm anti-b19 antibodies to human parvovirus b19 (b19) and b19 dna were positive, and bone marrow analysis revealed pure red cell aplasia with giant proerythroblasts. high dose gamma globulin was administered and the daily dose of steroi ... | 2003 | 12857057 |
| relevance of b19 markers in serum samples for a diagnosis of parvovirus b19-correlated diseases. | in order to evaluate the optimal and essential diagnostic test(s) for a correct diagnosis of b19 diseases, 344 consecutive serum samples were tested from 344 patients with clinical suspicion of b19 infection during an epidemic period (early spring-autumn 2000). sera were tested for b19 dna by a standardized competitive polymerase chain reaction-enzyme-linked immunosorbent assay (pcr-elisa) and dot-blot hybridization and for specific igm and igg by elisa. of 344 patients examined, 125 were positi ... | 2003 | 12858419 |
| acute parvovirus b19 infection associated with myocarditis in an immunocompetent adult. | inflammatory heart disease is causally linked with progressive left ventricular dysfunction and congestive heart failure. in childhood, infection with parvovirus b19 (pvb19) is usually benign, causing erythema infectiosum. however, severe fetal pvb19 infection may be associated with hydrops fetalis and fetal death caused by myocarditis. here we report a pvb19-induced myocarditis in a previously healthy 37-year-old patient admitted to the hospital because of chest pain and dyspnea due to left ven ... | 2003 | 12874772 |
| tumor necrosis factor (tnf)alpha and its soluble receptor (stnfr) p75 during acute human parvovirus b19 infection in children. | human parvovirus b19 (hpv) has been shown to be involved in the pathogenesis of various connective tissue and autoimmune diseases. in order to gain more information on hpv possible role in these diseases, we have investigated some immune responses in patients with acute hpv infection, mainly the secretion of the proinflammatory cytokine tumor necrosis factor (tnf)alpha and it's antagonist--the soluble tnf receptor (stnfr) p75. thirteen children with acute hpv infection and 13 healthy volunteers ... | 2003 | 12880679 |
| renal involvement in human parvovirus b19 infection. | | 2003 | 12883967 |
| anemia in children after transplantation: etiology and the effect of immunosuppressive therapy on erythropoiesis. | anemia in children after renal transplantation is more common than previously appreciated. multiple factors appear to play roles in the development of post-transplant anemia, the most common of which is absolute and/or functional iron deficiency anemia. most experts recommend that iron limited anemias in transplant patients should be diagnosed using the same criteria as for chronic renal failure patients. serum erythropoietin (epo) levels are expected to normalize after a successful renal transp ... | 2003 | 12890002 |
| relationship between human parvovirus b19 infection and aplastic anemia. | to explore the relationship between human parvovirus b19 (hpv b19) infection and aplastic anemia (aa) and to investigate the role of hpv b19 in the occurrence of aa. | 2001 | 12899333 |
| primary human parvovirus b19 infection in an hiv infected patient on highly active antiretroviral therapy. | adults developing primary human parvovirus b19 (b19) infection may present with arthralgia, fever, and maculopapular rash. recovery is linked to the development of specific neutralising antibodies. in immunosuppressed patients, including those with hiv infection, such humoral responses are impaired and severe chronic bone marrow suppression and arthritis may occur. | 2003 | 12902591 |
| safety of the new generation recombinant factor concentrates. | haemophilia a and b are x-linked disorders resulting from deficiency of factor viii and ix, respectively. clinical sequellae of factor viii or ix deficiency include spontaneous and traumatic haemorrhages into joints, soft tissues, and muscles. the cornerstone of therapy has been replacement of the deficient factor, historically with pooled-plasma derivatives. the unfortunate blood-borne infection transmission (such as hiv, hepatitis b and c viruses), inhibitor formation, immunosuppression, and, ... | 2002 | 12904137 |
| alpha5beta1 integrin as a cellular coreceptor for human parvovirus b19: requirement of functional activation of beta1 integrin for viral entry. | replication of the pathogenic human parvovirus b19 is restricted to erythroid progenitor cells. although blood group p antigen has been reported to be the cell surface receptor for parvovirus b19, a number of nonerythroid cells, which express p antigen, are not permissive for parvovirus b19 infection. we have documented that p antigen is necessary for parvovirus b19 binding but not sufficient for virus entry into cells. to test whether parvovirus b19 utilizes a cell surface coreceptor for entry, ... | 2003 | 12907437 |
| blood products and parvovirus b19. | a committee of the health council of the netherlands has expressed its opinion on introducing testing of blood products for parvovirus b19 (b19). although infections with b19 generally run their course without any serious health problems, for some groups, such as pregnant women, patients with underlying haematological problems and patients with immunodeficiency, infections with b19 can result in serious complications. for cellular blood products, which are derived either from a single donor or a ... | 2003 | 12916540 |
| detection of parvovirus b19 capsid proteins in lymphocytic cells in synovial tissue of autoimmune chronic arthritis. | the pathogenic influence of viral agents in chronic inflammatory joint diseases like rheumatoid arthritis has been discussed for many years. more recently, dna of several viruses, among them parvovirus b19 (b19), was traceable by pcr analysis in synovial fluid and synovial tissue. to investigate the potential role of parvovirus b19 in rheumatoid arthritis, we analyzed the expression of b19 vp1/vp2 proteins by immunohistochemistry in paraffin sections of 63 synovial specimens in rheumatoid arthri ... | 2003 | 12920226 |
| molecular pathology of inflammatory cardiomyopathy. | endomyocardial biopsy (emb) is often performed in patients presenting with sudden onset of heart failure to identify myocarditis. the introduction of immunohistochemical techniques for the detection and differentiation of infiltrating immune cells, specific adhesion molecules and mhc class i and ii molecules increased the prognostic value of emb in the diagnosis of myocarditis considerably. a major breakthrough in the understanding of pathogenetic mechanisms in myocarditis was achieved by diagno ... | 2004 | 12920583 |
| detection of ige anti-parvovirus b19 and increased cd23+ b cells in parvovirus b19 infection: relation to th2 cytokines. | the immune profile of a parvovirus b19-infected patient (male, 8 years old) was studied on day 0 (initial presentation) and on days 14 and 210 post symptom presentation (psp). before infection, the patient was skin test positive to various allergens, including ragweed and tree and grass pollens, and had a serum ige level of 150 iu/ml. on day 0, the patient was diagnosed as parvovirus b19 infected, as judged by the presence of igg anti-parvovirus abs in serum (eia) and presentation of "slap cheek ... | 2003 | 12921761 |
| parvovirus b19 infection mimicking acute myocardial infarction. | enteroviruses (evs) and adenoviruses (advs) have been considered common causes of myocarditis and dilated cardiomyopathy. in the present study, we report on the association of parvovirus b19 (pvb19) genomes in the clinical setting of acute myocarditis. | 2003 | 12925460 |
| b19 virus genome diversity: epidemiological and clinical correlations. | genetic analysis of parvovirus b19 has been carried out mainly to establish a framework to track molecular epidemiology of the virus and to correlate sequence variability with different pathological and clinical manifestations of the virus. a good amount of information regarding b19 virus sequence variability is available, and presently there are about 400 sequence records deposited in the nucleotide database of ncbi. a few are almost complete genomic sequences, and these allow the construction ... | 2003 | 12927746 |
| stability of minute virus of mice against temperature and sodium hydroxide. | treatment with steam and/or dilute naoh are commonly used techniques to disinfect manufacturing vessels and tools in the pharmaceutical industry. the aim of this procedure is sanitisation and inactivation of microbiological and viral contaminants. here we describe the inactivation of the mouse parvovirus minute virus of mice (mvm) under these conditions. parvoviruses are known to be resistant to physico-chemical treatment and one representative of this family, the human parvovirus b19, is a pote ... | 2003 | 12935806 |
| purpuric gloves and socks syndrome caused by parvovirus b19 infection. | | 2003 | 12938681 |
| cytokines in parvovirus b19 infection as an aid to understanding chronic fatigue syndrome. | human parvovirus b19 infection has been associated with various clinical manifestations of a rheumatic nature such as arthritis, fatigue, and chronic fatigue syndrome (cfs), which can persist for years after the acute phase. the authors have demonstrated recently that acute b19 infection is accompanied by raised circulating levels of il-1b, il-6, tnf-a, and ifn-g and that raised circulating levels of tnf-a and ifn-g persist and are accompanied by mcp-1 in those patients who develop cfs. a resolu ... | 2003 | 12946285 |
| prevalence of viral dna in amniotic fluid of low-risk pregnancies in the second trimester. | the association between fetal viral infection and adverse pregnancy outcome is well documented. however, the prevalence of common viral pathogens in the amniotic fluid of normal pregnancies is not established. the purpose of this study was to determine this prevalence in asymptomatic patients. | 2003 | 12962262 |
| establishment of multifunctional monoclonal antibody to the nonstructural protein, ns1, of human parvovirus b19. | ns1 expression of human parvovirus b19 is a critical event in b19 pathogenesis. however, the mechanisms of ns1-induced cytotoxicity in b19 infection have yet to be clarified mainly because of the absence of multifunctional monoclonal antibodies (mab) against ns1. the purpose of this study was to establish such mab, which function in immunoprecipitation assays, immunoblotting, indirect immunofluorescence, and immunohistochemical staining. | 2003 | 12963386 |
| virus-like particle production at low multiplicities of infection with the baculovirus insect cell system. | the baculovirus insect cell expression system (bevs) was used for the production of self-forming porcine parvovirus-like particles (vlps) in serum-free medium. a low multiplicity of infection (moi) strategy was used to overcome an extra virus amplification step, undesirable in industrial production, and to minimize the virus passage effect. it was confirmed that the time of infection (toi) and moi are dependent variables. higher cell densities were obtained at low mois, keeping a constant toi; h ... | 2003 | 12966582 |
| the presence of parvovirus b19 vp and ns1 genes in the synovium is not correlated with rheumatoid arthritis. | to amplify both ns1 and vp genes of parvovirus b19 dna in synovial membrane (sm) and serum obtained from patients with rheumatoid arthritis (ra) and to analyze whether the presence of viral dna is correlated with synovitis. | 2003 | 12966589 |
| cytokine gene polymorphisms associated with symptomatic parvovirus b19 infection. | the immune system has been implicated in the pathogenesis of certain clinical manifestations of parvovirus b19 infection, including rash and arthralgia. cytokines feature in the pathogenesis of parvovirus b19 infection, so inherited variability in cytokine responses to b19 infection might have a bearing on the symptomatology of parvovirus b19 infection. | 2003 | 14514772 |
| [anemia associated with parvovirus b19 infecion in a young adult without underlying disease]. | | 2003 | 14516275 |
| acute parvovirus b19 infection mimicking chronic fatigue syndrome. | a japanese woman developed prolonged fatigue, neck and shoulder pain, headache, pyrexia, insomnia, anorexia, lymphadenopathy, and diarrhea for two months. she had experienced various stressors before these symptoms developed. serological test demonstrated that she had acute parvovirus b19 infection. major depressive disorder was also diagnosed by a psychiatrist. her symptoms disappeared after administration of selective serotonin reuptake inhibitors and oriental herbs, although human parvovirus ... | 2003 | 14518687 |
| epidemiology of human parvovirus b19 in children with sickle cell disease. | human parvovirus (hpv) b19 causes significant morbidity and mortality in children with sickle cell disease (scd), but little data are published about the epidemiology of hpv b19 infection and its associated complications in this patient population. in this study, prevalence and incidence rates of hpv b19 were determined in 633 patients with scd followed at the children's hospital of philadelphia between november 1996 and december 2001. thirty percent (30%) were hpv b19 immunoglobulin g (igg) pos ... | 2004 | 14525777 |
| commentary on: dettmeyer r, kandolf r, baasner a, banaschak s, eis-hubinger am, madea b. fatal parvovirus b19 myocarditis in an 8-year-old boy. j forensic sci 2003;48(1): 183-186. | | 2003 | 14535705 |
| influenza b pneumonia with staphylococcus aureus superinfection associated with parvovirus b19 and concomitant agranulocytosis. | an 11-year-old patient with anamnestic fever for 3 days and signs of upper respiratory tract infection underwent fulminant staphylococcus aureus pneumonia with concomitant agranulocytosis. from autopsia influenza b virus and parvovirus b19 were detected by nucleic acid amplification technique (nat). specific igg but no igm points to preexisting parvovirus b19 infection. whether in this case agranulocytosis can be interpreted as early manifestation of reactivated parvovirus b19 infection is under ... | 2003 | 14556063 |
| parvovirus b19 infection in taiwanese patients with hematological disorders. | human parvovirus b19 has a strong tissue tropism for erythroid progenitor cells and is a causative agent for anemia. however, it remains unclear whether patients with hematological disorders are at a higher risk of b19 infection. in the present study, we evaluated the prevalence of b19 infection in 278 patients with hematological disorders by indirect antibody capture enzyme-linked immunosorbent assay. virus inoculation into cell culture (of tf-6, wrl-68, and hl-60) was carried out using serum f ... | 2003 | 14556276 |
| pcr-based diagnosis of enterovirus and parvovirus b19 in paraffin-embedded heart tissue of children with suspected sudden infant death syndrome. | the diagnosis of viral myocarditis remains difficult and generally depends on clinical and histologic criteria. viral cultures and serology are often unrewarding with low yields. the purpose of this study was to analyze the usefulness of pcr in the rapid diagnosis of myocarditis in children. pcr was used to analyze 120 myocardial tissue samples from 60 cases of sudden infant death syndrome (sids) and 56 myocardial tissue samples from 36 cases with well-known causes of sudden death (11 children y ... | 2003 | 14563946 |
| [studies on the novel association of human herpesvirus-7 with skin diseases]. | human herpesvirus 7 in pityriasis rosea, this and other viruses in papular-purpuric gloves-and-socks syndrome have been implicated, but their primary or recurrent infections are still in question. | 2003 | 14564923 |
| seroprevalence of human parvovirus b19 infection in an urban population in malaysia. | a seroepidemiological study carried out on 800 stored serum samples collected between january 1999 to december 2000 derived from an urban population in malaysia showed that the overall seropositive rate of human paravovirus b19 infection was 37.6%, with an overall geometric mean titre (gmt) of 18.3 iu. the seropositive rates of b19 among the male and female populations were 39.0% and 36.3% respectively. the seropositive rates among the racial groups were 37.2%, 38.2%, 38.1% and 29.4% respectivel ... | 2002 | 14569725 |
| [parvovirus b19 infection after renal transplantation]. | despite improvements in the management of transplanted patients, viral infections following transplantation remain significant causes of morbidity and mortality. new laboratory techniques have improved the diagnosis of pathogenic viral infections following transplantation such as parvovirus b19 infections. anemia is the principal abnormality associated with parvovirus b19 infection but other complications have been reported such as hepatitis, glomerulonephritis, myocarditis or arthritis. in immu ... | 2003 | 14584298 |
| [exanthematous outbreak caused by human parvovirus b19 in a school in banderaló, argentina]. | we report an exanthematic outbreak in a school of banderaló town, located in the district of general villegas, buenos aires province, argentina. this outbreak affected 21 individuals, with a wide range of age (out of a total population of 1400). the observed clinical manifestations were exanthema and in some cases fever, asthenia, arthropathies and myalgia. the presence of specific igm antibodies against measles, rubella and human parvovirus b19 were investigated. analysis of igm antibodies to p ... | 2003 | 14587381 |
| primary human herpesvirus-6 and -7 infections, often coinciding, misdiagnosed as measles in children from a tropical region of brazil. | we investigated primary human herpesvirus-6 and -7 (hhv-6, hhv-7) infections as a cause of rashes incorrectly diagnosed as measles in brazilian children. sera from 124 patients, aged 4 months to 17 years, from the states of rio de janeiro and espirito santo, in whom measles, rubella and parvovirus b19 infections had been excluded, were studied using indirect immunofluorescence antibody avidity tests; 38 (31%) had evidence of primary hhv-6 and/or hhv-7 infections. twenty four children had primary ... | 2003 | 14596528 |
| association of acute parvovirus b19 infection with new onset of acute lymphoblastic and myeloblastic leukaemia. | to investigate the association of acute parvovirus b19 infection with new onset of acute lymphoblastic and myeloblastic leukaemia. | 2003 | 14600138 |
| role of parvovirus b19 infection in juvenile chronic arthritis. is more investigation needed? | | 2003 | 14611135 |
| quick preparations of human parvovirus b19 microarray probes using pcr. | to prepare dna microarray probes for the detection of human parvovirus b19. method specific pcr primers were designed with the primer premier 5.0 to amplify the conserved regions of human parvovirus b19 genome. the pcr products were cloned into the pmd-18 t vector. | 2003 | 14625164 |
| parvovirus b19 and the pathogenesis of anaemia. | human parvovirus b19 (b19) infection causes human bone marrow failure, by affecting erythroid-lineage cells which are well-known target cells for b19. the anaemia induced by b19 infection is of minor clinical significance in healthy children and adults, however, it becomes critical in those afflicted with haemolytic diseases. this condition is called transient aplastic crisis, and the pathogenesis is explained by the short life-span of red blood cells. similarly, fetuses are thought to be severe ... | 2003 | 14625883 |
| three cases of myocarditis in childhood associated with human parvovirus (b19 virus). | we report three childhood cases of myocarditis associated with human parvovirus (b19 virus). all three children presented with significant cardiac decompensation, with one requiring extracorporeal membrane oxygenation support. left ventricular function was severely impaired in all three. myocardial biopsy confirmed histological myocarditis and was positive for b19 virus by nested polymerase chain reaction. serum was positive for igg b19 virus but negative for igm in all three cases. all three ch ... | 2003 | 14627316 |
| pure red cell aplasia associated with parvovirus b19 infection in a patient with ulcerative colitis. | anemia is a common problem that results from various causes in patients with ulcerative colitis (uc), but there is little information on the association of uc with pure red cell aplasia (prca). we describe the first case of parvovirus-induced prca in uc. a 28-year-old woman with chronic uc was admitted to the hospital for treatment of active pancolitis. three courses of pulse therapy with methylprednisolone provided complete remission. however, the patient developed reticulocytopenia and a subse ... | 2003 | 14627362 |
| [a case of acute hepatitis associated with parvovirus b19]. | | 2003 | 14639929 |
| [aplastic crisis in sickle cell anemia induced by parvovírus b19] | purpose: transient aplastic crisis is reported in an eight-month old child with sickle cell anemia and acute b19 parvovirus infection. this fact is uncommon in this age. patient and methods: the authors review the literature and describe a clinical case of an eight-month old child with sickle cell anemia presented with profound anemia and reticulocytopenia. his peripheral blood was analyzed for parvovirus b19 using the polymerase chain reaction (pcr), and for anti b19 immunoglobulin ig m, and ig ... | 2000 | 14647635 |
| [transient pancytopenia induced by parvovirus b19 in a child with hereditary spherocytosis] | objective: to describe the occurrence of transient pancytopenia induced by parvovirus b19 infection in a patient with hereditary hemolytic anemia and to discuss the importance of the diagnosis of this pathology.methods: case report of a child whose diagnosis was made by polymerase chain reaction (pcr) and serology, and review of the literature.clinical report: a twelve year-old male patient with hereditary spherocytosis, presenting non-specific symptoms of an infectious syndrome followed by seve ... | 2000 | 14647664 |
| [parvovirus b19 infection in pregnancy]. | | 2003 | 14655561 |
| the current status of immune modulating therapy for myocarditis: a case of acute parvovirus myocarditis treated with intravenous immunoglobulin. | we present a case of a patient who presented with a febrile illness without obvious source initially, who developed profound cardiac decompensation and left ventricular dysfunction. viral titers obtained during the course of illness confirmed parvovirus infection. intravenous aggressive immunoglobulins and medical therapy for heart failure resulted in stabilization and, ultimately, a complete recovery. recent data from clinical trials are discussed regarding the utility of immunoglobulins in the ... | 2003 | 14671501 |
| parvoviral infection of endothelial cells and stromal fibroblasts: a possible pathogenetic role in scleroderma. | systemic sclerosis (ssc) is a connective tissue disease (ctd) which differs from other ctds by progressive irreversible fibrosis in lung, kidney, skin, and heart. it has a worse prognosis compared to several other ctds. the pathogenesis may reflect a humorally mediated microangiopathy in concert with the overproduction of collagen triggered by immune-mediated cytokine production. having previously demonstrated parvovirus b19 (b19) dna in bone marrow and skin biopsies of ssc patients in the absen ... | 2004 | 14675284 |
| detection of parvovirus b19 igg: choice of antigens and serological tests. | serum samples were analysed for the presence of (a) igg against vp1+vp2 using recombinant native conformational antigens by elisa test (b) igg against vp2 using recombinant native conformational antigens by elisa test and (c) igg against vp1 and against vp2 using denatured linear antigens by western blot. out of the 446 samples examined, 353 were positive for specific b19 igg and out of these, 98.6 % proved positive in the elisa assay using conformational vp1+vp2 antigens, 94.6% proved positive ... | 2004 | 14675870 |
| clinical presentation of parvovirus b19 infection in children with aplastic crisis. | the records of 22 children with parvovirus b19-induced aplastic crisis were reviewed. the group consisted of 16 children with sickle cell hemoglobinopathies and 6 with hereditary spherocytosis. children presented to the hospital 0.5 to 8 days (mean, 2.4 days) after the onset of symptoms. the children with sickle-cell disease presented earlier (mean, 1.4 days) than did children with hereditary spherocytosis (mean, 5 days; p = 0.02. fever was the most common symptom, occurring in 73% of children. ... | 2003 | 14688575 |
| frequent detection of parvovirus b19 genome in the myocardium of adult patients with idiopathic dilated cardiomyopathy. | aside from enteroviruses and other viruses, e.g., adenoviruses, which are known to be associated with idiopathic dilated cardiomyopathy (idc), a cardiac tropism is also attributed to parvovirus b19 (pvb19). the purpose of the present study was to determine the prevalence of enterovirus, adenovirus and pvb19 genomes in the myocardium of adult patients with idc and to analyze the significance of pvb19 with regard to the course of the disease, as compared to the other cardiotropic viruses. in 52 ad ... | 2004 | 14689308 |
| quantitative real-time detection of parvovirus b19 dna in plasma. | as of 2004, the european pharmacopoeia demands that plasma pools for production of anti-d immunoglobulin should not contain more than 104 iu per ml of parvovirus b19 (b19v) dna. hence, before pooling, highly viremic donations have to be identified, and after pooling the level of b19v dna must be determined. the performance of a new real-time b19v dna pcr test (roche, mannheim, germany) was studied, using a dna extractor (nuclisens, biomerieux, boxtel, the netherlands) for isolation of nucleic ac ... | 2004 | 14692974 |
| parvovirus infects cardiac myocytes in hydrops fetalis. | parvovirus infection during pregnancy is an important cause of hydrops fetalis. it is attributed to anemia caused by viral-induced destruction of red blood cells. infection of other organs has been reported including the heart, liver, and lungs. few of these reports, however, convincingly demonstrate virions within the functional parenchyma of the tissue. this is of particular concern regarding myocardium in the context of hydrops fetalis which is, in part, due to cardiac failure. the problem in ... | 2003 | 14708734 |