| dna in cauliflower mosaic virus. | | 1968 | 5669985 |
| purification of cauliflower mosaic virus. | | 1960 | 13814596 |
| purification and properties of cauliflower mosaic virus. | | 1960 | 14433226 |
| seed tolerance to deterioration in arabidopsis is affected by virus infection. | seed longevity is the period during which the plant seed is able to germinate. this property is strongly influenced by environment conditions experienced by seeds during their formation and storage. in the present study we have analyzed how the biotic stress derived from the infection of cauliflower mosaic virus (camv), turnip mosaic virus (tumv), cucumber mosaic virus (cmv) and alfalfa mosaic virus (amv) affects seed tolerance to deterioration measuring germination rates after an accelerated ag ... | 2017 | 28477474 |
| matrix metalloproteinases operate redundantly in arabidopsis immunity against necrotrophic and biotrophic fungal pathogens. | matrix metalloproteinases (mmps) are evolutionarily conserved and multifunctional effector molecules playing pivotal roles in development and homeostasis. in this study we explored the involvement of the five arabidopsis thaliana at-mmps in plant defence against microbial pathogens. expression of at2-mmp was most responsive to inoculation with fungi and a bacterial pathogen followed by at3-mmp and at5-mmp, while at1-mmp and at4-mmp were non-responsive to these biotic stresses. loss-of-function m ... | 2017 | 28832648 |
| the arabidopsis phytocystatin atcys5 enhances seed germination and seedling growth under heat stress conditions. | phytocystatins (phycyss) are plant-specific proteinaceous inhibitors that are implicated in protein turnover and stress responses. here, we characterized a phycys from arabidopsis thaliana, which was designated atcys5. rt-qpcr analysis showed that the expression of atcys5 in germinating seeds was induced by heat stress (hs) and exogenous abscisic acid (aba) treatment. analysis of the expression of the β-glucuronidase reporter gene under the control of the atcys5 promoter showed that atcys5 expre ... | 2017 | 28756655 |
| evaluation of viral surrogate markers for study of pathogen dissemination during simulations of patient care. | during patient care simulations, cauliflower mosaic virus dna and bacteriophage ms2 performed similarly as surrogate markers of pathogen dissemination. these markers disseminated to the environment in a manner similar to clostridium difficile spores but were more frequently detected on skin and clothing of personnel after personal protective equipment removal. | 2017 | 28752103 |
| plant infection by two different viruses induce contrasting changes of vectors fitness and behavior. | insect-vectored plant viruses can induce changes in plant phenotypes, thus influencing plant-vector interactions in a way that may promote their dispersal according to their mode of transmission (i.e., circulative vs. noncirculative). this indirect vector manipulation requires host-virus-vector coevolution and would thus be effective solely in very specific plant-virus-vector species associations. some studies suggest this manipulation may depend on multiple factors relative to various intrinsic ... | 2017 | 28731285 |
| a novel sugarcane bacilliform virus promoter confers gene expression preferentially in the vascular bundle and storage parenchyma of the sugarcane culm. | saccharum species such as sugarcane and energy cane are key players in the expanding bioeconomy for sugars, bioenergy, and production of high-value proteins. genomic tools such as culm-regulated promoters would be of great value in terms of improving biomass characteristics through enhanced carbon metabolism for sugar accumulation and/or fiber content for biofuel feedstock. unlike the situation in dicots, monocot promoters currently used are limited and mostly derived from highly expressed const ... | 2017 | 28680479 |
| a putative antiviral role of plant cytidine deaminases. | a mechanism of innate antiviral immunity operating against viruses infecting mammalian cells has been described during the last decade. host cytidine deaminases ( e.g., apobec3 proteins) edit viral genomes, giving rise to hypermutated nonfunctional viruses; consequently, viral fitness is reduced through lethal mutagenesis. by contrast, sub-lethal hypermutagenesis may contribute to virus evolvability by increasing population diversity. to prevent genome editing, some viruses have evolved prote ... | 2017 | 28620460 |
| expression of four s. pneumoniae type 2 polysaccharide biosynthetic enzymes utilising the endogenous kex2 protease activity in tobacco. | in order to express multisubunit proteins, or to manipulate metabolic pathways in plants it is essential to be able to efficiently express multiple proteins within the same plant cell. to increase the efficiency of multi-protein expression, we demonstrate the use of the golgi localized kex2 protease activity in tobacco to process a large polyprotein precursor consisting of four individual protein domains into its individual protein constituents. four genes encoding enzymes involved in the biosyn ... | 2017 | 28649068 |
| a 64-bp sequence containing the gaaga motif is essential for camv-35s promoter methylation in gentian. | this study investigated sequence specificity and perenniality of dna methylation in the cauliflower mosaic virus (camv) 35s promoter of transgenic gentian (gentiana triflora×g. scabra) plants. unlike conventional transgene silencing models, 35s promoter hypermethylation in gentian is species-specific and occurs irrespective of the t-dna copy number and genomic location. modified 35s promoters were introduced into gentian, and single-copy transgenic lines were selected for methylation analysis. m ... | 2017 | 28624556 |
| capped antigenomic rna transcript facilitates rescue of a plant rhabdovirus. | recovery of recombinant negative-stranded rna viruses from cloned cdnas is an inefficient process as multiple viral components need to be delivered into cells for reconstitution of infectious entities. previously studies have shown that authentic viral rna termini are essential for efficient virus rescue. however, little is known about the activity of viral rnas processed by different strategies in supporting recovery of plant negative-stranded rna virus. | 2017 | 28610585 |
| dissemination of a nonpathogenic viral dna surrogate marker from high-touch surfaces in rooms of long-term care facility residents. | a nonpathogenic dna marker inoculated onto the television remote controls in rooms of 2 ambulatory long-term care facility (ltcf) residents disseminated to the hands of the ltcf residents, to high-touch surfaces in the room and on the ward, and to shared portable equipment. these findings suggest that contaminated high-touch surfaces in rooms of ambulatory ltcf residents are a potential source for widespread dissemination of pathogens. | 2017 | 28526313 |
| water deficit enhances the transmission of plant viruses by insect vectors. | drought is a major threat to crop production worldwide and is accentuated by global warming. plant responses to this abiotic stress involve physiological changes overlapping, at least partially, the defense pathways elicited both by viruses and their herbivore vectors. recently, a number of theoretical and empirical studies anticipated the influence of climate changes on vector-borne viruses of plants and animals, mainly addressing the effects on the virus itself or on the vector population dyna ... | 2017 | 28467423 |
| overexpression of psp1 enhances growth of transgenic arabidopsis plants under ambient air conditions. | the importance of the phosphorylated pathway (ppsb) of l-serine (ser) biosynthesis in plant growth and development has been demonstrated, but its specific role in leaves and interaction with photorespiration, the main leaf ser biosynthetic pathway at daytime, are still unclear. to investigate whether changes in biosynthesis of ser by the ppsb in leaves could have an impact on photorespiration and plant growth, we overexpressed psp1, the last enzyme of this pathway, under control of the cauliflow ... | 2017 | 28455648 |
| isolation and characterization of ulva prolifera actin1 gene and function verification of the 5' flanking region as a strong promoter. | ulva prolifera is a green macroalgae with an extremely high growth rate that can accumulate biomass with considerable protein content. to set up an available seaweed expression system, a prior step is to isolate endogenous and strong constitutive promoters. for this reason, the full-length genomic actin1 gene from u. prolifera (upactin1) was cloned and its 5' flanking sequence was obtained by genome walking. the upactin1 open reading frame consisted of 1134 nucleotides encoding 377 amino acid re ... | 2017 | 28453384 |
| proteomic composition of the acrostyle: novel approaches to identify cuticular proteins involved in virus-insect interactions. | the acrostyle is a distinct anatomical region present on the cuticle at the inner face of the common food/salivary canal at the tip of aphid maxillary stylets. this conserved structure is of particular interest as it harbors the protein receptors of at least 1 plant virus, cauliflower mosaic virus, and presumably has other roles in plant-insect interactions. previously we reported immunolabeling of a highly conserved motif of cuticular proteins from the cpr family (named for the presence of a re ... | 2017 | 28421675 |
| a quantitative pcr-electrochemical genosensor test for the screening of biotech crops. | the design of screening methods for the detection of genetically modified organisms (gmos) in food would improve the efficiency in their control. we report here a pcr amplification method combined with a sequence-specific electrochemical genosensor for the quantification of a dna sequence characteristic of the 35s promoter derived from the cauliflower mosaic virus (camv). specifically, we employ a genosensor constructed by chemisorption of a thiolated capture probe and p-aminothiophenol gold sur ... | 2017 | 28420193 |