| myelin basic protein in frozen and unfrozen bovine brain: a study of autolytic changes in situ. | | 1975 | 51045 |
| ultrastructure of the invasion of eimeria magna sporozoites into cultured cells. | monolayers of bovine kidney cells were overlaid with eimeria magna sporozoites and observed with phase-contrast optics until penetration of the cells by the parasites had begun. cells and penetrating parasites were fixed with glutaraldehyde and oso4-containing ruthenium red, dehydrated, and embedded in situ. cells being penetrated were selected for study in the electron microscope. the lack of intracellular staining with ruthenium red and intact plasmalemmas of cells being penetrated, was acc ... | 1975 | 51083 |
| letter: calf diarrhoea coronavirus. | | 1975 | 51327 |
| toxic and photodynamic effects of vital staining with cresyl blue on bull spermatozoa. | | 1975 | 51485 |
| evaluation of an amidolytic heparin cofactor assay method. | | 1975 | 51519 |
| improved zymographic detection of bovine acrosin. | | 1975 | 51596 |
| the foamy viruses. | | 1975 | 51620 |
| morphological, chemical, and antigenic organization of mammalian c-type viruses. | new features in the architecture of mammalian type c viruses, in particular knoblike surface projections and hexagonally arranged subunits on the core shell could be demonstrated by electron microscopy, taking advantage of newly developed preparation techniques. as examples, murine leukemia viruses (mulvs) and newly isolated porcine and bovine c viruses are presented. the major proteins of a mulv were isolated and partially characterized in chemical terms and with respect to their serological an ... | 1975 | 51634 |
| further studies on the antigenic properties and distribution of the putative bovine leukemia virus. | the c-type viruses found in long-term cultures. new bolton center (nbc) cell lines, of peripheral lymphocytes from leukemic cattle and in short-term cultures of bovine buffy coat(bc) cells share an immunofluorescent(if)antigen detected in the cytoplasm of infected cells as well as an antigen demonstrable in gel diffusion experiments. therefore the viruses from these cultures most likely represent different isolates of the putative bovine leukemia virus (blv). the blv precipitin antigen is analog ... | 1975 | 51637 |
| inhibitory effects of superoxide dismutases and various other proteins on the nitroblue tetrazolium reduction by phagocytizing guinea pig polymorphonuclear leukocytes. | | 1975 | 51640 |
| solution behavior, circular dichroism and 22 hmz pmr studies of the bovine myelin basic protein. | bovine myelin basic protein has been investigated with regard to its solution behavior, circular dichroism and 220 mhz pmr spectral properties. at ph 4.8 gamma/2=0.1 acetate buffer, light scattering yielded a mr of 17 700 and a virial coefficient of 1.0-10(-4) mol-ml/g2. above ph 7.0 the protein was found to aggregate to higher mol. wt species. sedimentation experiments at ph 4.8 yielded s degrees 20,w of 1.27 s at gamma/2=0.1 and 1.46 s at gamma/2=0.35. the diffusion coefficient determined from ... | 1975 | 51651 |
| the influence of complement on the neutralization of infectious bovine rhinotracheitis virus by globulins derived from early and late bovine antisera. | calves were inoculated at four week intervals with infectious bovine rhinotracheitis virus (ibrv). sera were obtained at eight days (early sample) and 21 days (late sample) after each inoculation. kinetic neutralization was carried out with 7s and 19s globulins derived from these sera, both in the presence and in the absence of guinea pig complement (c). in all instances the 19s neutralizing antibody (ab) was dependent on c for neutralization of ibvr. however, c dependency was not observed with ... | 1975 | 51677 |
| effects of barbiturates on ultrastructure and polymerization of microtubules in vitro. | barbiturates were examined for in vitro effects on ultrastructure of the frog sciatic system and polymerization of microtubules (mt) in a brain supernatant. exposure for 5-17 h to 2.0 mm barbiturates caused a considerable loss of mt in ganglionic cell bodies and sciatic axons. this was mostly followed by a proliferation of 10 nm filaments. under similar conditions treatment with 1 mm nacn or 0.1 mm 2,4-dnp did not change the number or ultrastructure of mt and filaments. eight barbiturates, varyi ... | 1975 | 51688 |
| [metabolism and in vitro binding of several organochlorine and organophosphate pesticides to calf thymus dna and rat liver microsomal proteins]. | the organophosphorus insecticide ethyl-parathion was transformed by rat liver microsomes into metabolites which were bound to calf thymus dna, in large amount. when the rats were treated by phenobarbital or 3-methylcholanthrene, the metabolite binding to dna was increased two-fold. by contrast, in the same conditions, the organochlorine insecticides, aldrin, dieldrin and gamma hexachlorocyclohexane (lindane), did not yield metabolites able to bind to dna and to proteins. | 1975 | 51693 |
| distinct ribonuclease h activities in calf thymus. | three enzymes with ribonuclease h activity are present in calf thymus. they have been separated on the basis of chromatographic behaviour and molecular weight. they are further distinguished from one another by their ionic requirements and sensitivity to the -sh reagent n-ethylmaleimide. two of these enzymes are classified as ribonuclease h, the third is obtained in a fraction which possesses ribonuclease h activity but also degrades double-stranded rna and poly(ra). no association between any o ... | 1975 | 51794 |
| non-helical sequences of rabbit collagen. correlation with antigenic determinants detected by rabbit antibodies in homologous regions of rat and calf collagen. | non-helical peptide fragments were isolated from rabbit skin collagen after cleavage of alpha chains with cyanogen bromide and proteases. determination of their amino acid sequence indicated a length of 9, 16 and 25 amino acid residues for the non-helical sequences located in the n-terminal region of alpha2 and alpha1 chain and in the c-terminal region of alpha1 chain, respectively. the c-terminal sequence tyr-tyr hitherto considered as the genuine end of collagen alpha1 chain is in part of rabb ... | 1975 | 51796 |
| use of electron microscopy for detection of viral and other microbial contaminants in bovine sera. | a total of 25 lots of bovine serum samples were pelleted in beem capsules for thin sectioning and were examined by electron microscopy. these included 17 lots of fetal bovine serum pools and five lots of calf serum pools obtained from commercial sources, and three lots of adult bovine serum from local dairy farms. virus-like particles, 50 to 300 nm in diameter, were detected in 17 of 25 (68%) of the sera. five of 25 serum samples showed the presence of mycoplasma-like agents. incubation of bovin ... | 1975 | 51855 |
| glycogenolysis and control of anaphylactic histamine release by cyclic adenosine monophosphate--related agents. | the relationship of glycogen and glucose to anaphylactic histamine release from chopped sensitized guinea pig lung in vitro was studied. a parallelism was observed between the total amount of glycogen in the sensitized lung and the total amount of histamine released from the lung by antigen-antibody reactions. removal of glucose from the medium for tissue suspension resulted in reduction in histamine release. depletion of glycogen and/or glucose from the system was associated with (1) abolition ... | 1975 | 51861 |
| stoichiometry of the aldehyde fuchsin staining reaction for proteins. | model systems of agar films containing known concentrations of bovine serum albumin and alpha-chymotrypsinogen were stained with aldehyde fuchsin after oxidation with acidified permanganate solution. these films were scanned in a scanning microphotometer to determine the mean extinction and the total extinction of predetermined areas. results indicate that the dye binds quantitatively to the proteins. blocking the acidic side groups of the proteins inhibited the binding of the dye. the degree of ... | 1975 | 51868 |
| a contribution to immunological specificity of dna in leukosis. | active antisera containing antibodies to deproteinized dna preparations of normal tissue and the spleen of cows suffering from myeloleukosis were obtained. the anti dna sera to dna preparations contained complement fixing antibodies related to gamma m globulins. in the study of leukosis and normal anti dna sera in quantitative cfr with the corresponding test antigens, immunological specificity of dna preparations isolated from the organs of cows affected with myeloleukosis was established. immun ... | 1975 | 51870 |
| carrier-induced tolerance to nucleic acid antigens. | balb/c and sjl mice were treated with nucleosides-igg1 as a tolerogen, before either primary or secondary immunization with nucleosides-keyhole limpet hemocyanin. nucleoside-specific responses were measured serologically by a modified farr assay, with either 14c-labeled denatured dna or nucleosides-131-i-labeled bsa as test antigen. specificity of the response was tested by hapten inhition experiments. multiple doses of nucleosides-igg1 tolerogen given before the primary or secondary immunizatio ... | 1975 | 51874 |
| antigenic analysis of chlamydiae by two-dimensional immunoelectrophoresis. ii. a trachoma-lgv-specific antigen. | two-dimensional immunoelectrophoresis was utilized to study precipitins in hyperimmune rabbit serum made against chlamydiae and from patients with chlamydial infections. an antigen of triton x-100-solubilized l2/434/bu organisms with an electrophoretic mobility of 0.65 relative to bovine serum albumin at ph 8.6 was excised from the agarose gel of electrophorograms as antigen-antibody complexes and used to immunize rabbits. a monospecific antiserum to antigen 0.65 was obtained that reacted with t ... | 1975 | 51883 |
| studies on the mechanism of suppression of delayed hypersensitivity by the antischistosomal compund niridazole. | niridazole given in a single oral dose of 100 mg/kg to guinea pigs sensitized to ortho-chlorobenzoyl chloride-bovine gamma-globulin (ocb-bgg) regularly abolished delayed cutaneous reactivity. little effect was observed, however, when cells from these animals were tested in vitro with either direct or indirect assays for migration inhibitory factor (mif). on the other hand, sera taken from nonsensitized guinea pigs after they had received 100 mg/kg of niridazole markedly diminished antigen-induce ... | 1975 | 51891 |
| morphological effects of osmolarity on purified noradrenergic vesicles. | large dense core vesicles (ldv) were purified from bovine splenic nerve homogenates by the sucrose-d2o density gradient method. vesicles were subjected to a 50% increase and decrease in osmolarity from the control 330 mosmol 1(-1) by adjusting sucrose or potassium phosphate buffer during pre- and/or postfixation. control vesicles with a mean diameter of 717 a readily swelled to approximately 1050 a and shrunk to approximately 600 a in the hypotonic and hypertonic media, respectively, with either ... | 1975 | 51907 |
| [biochemical properties of alpha-fetoprotein (author's transl)]. | | 1975 | 51943 |
| letter: ultrasound and mammalian d.n.a. | | 1975 | 52031 |
| letter: zinc deficiency and cellular immune deficiency in acrodermatitis enteropathica in man and zinc deficiency with thymic hypoplasia in fresian calves: a possible genetic link. | | 1975 | 52085 |
| disease-inducing activity of different parts of bovine encephalitogenic protein in guinea-pigs. | the encephalitogenic activity of different parts of bovine encephalitogenic protein (bep) has been tested in guinea-pigs, mainly at a dose equimolar to 32 mug of intact protein. a high activity was found with complete protein and with region 89-169; both peptide 43-115, tyr (modified at the tyrosine residue 67) and hnb-89-169 (modified at the tryptophan residue 115) showed a moderate but clear-cut activity, whereas that of peptide 1-42 was very faint. no qualitative differences in the disease in ... | 1975 | 52129 |
| f antigen. iii. immunological and biological properties. | relationship of the isoforms of f antigens from a2g and cba inbred mouse strains have been worked out in detail in respect to their immunogenicity. mice from several inbred strains have been shown to possess the ability of forming anti-f upon stimulation with xenogeneic f antigens from certain species. an immunoelectrophoretic method for sensitive quantitation of the antigen is described. during human ontogenesis the antigen appears very early. it could never be detected in serum except in cases ... | 1975 | 52137 |
| [preparation and properties of indomethacin-protein conjugates. 7. immunosuppressive antigen conjugates]. | | 1975 | 52161 |
| immunological recognition of specific antigenic determinants in uv- and gamma-irradiated phage dna. | thermally denatured dna of coliphage t1 after treatment with uv-light (2537 a) and 60co-gamma rays acts as a hapten with antigenic determinant groups specific for radiation-induced alterations of the macromolecule. after conjugation to methylated bovine serum albumin the dna becomes immunogenic in rabbits. antibodies against irradiated dna do not react with unirradiated single-stranded dna. antigen-antibody complexes were demonstrated by cscl-density gradient centrifugation. the decrease in buoy ... | 1975 | 52163 |
| [on the molecular species of mammalian cell dna polymerase (author's transl)]. | | 1975 | 52166 |
| some aspects of immunohistoperoxidase reactions in the agarose bead model system. | agarose beads that serve as matrices for antigens can be useful tools, not only for studying the quantitative aspects of immunohistoenzyme reactions but also for detecting antibodies. the present study focuses on localization of the ultimate reaction product within the beads; this factor is important when quantitative aspects of immunohistoenzyme reactions are under investigation. localization varies with respect to the concentration of cnbr used to activate the beads. in addition, a method is d ... | 1975 | 52312 |
| detection of structural differences between nuclear and mitochondrial glutamate dehydrogenases by the use of immunoadsorbents. | structural differences between crystalline mitochondrial and nuclear glutamate dehydrogenases from ox liver have been detected by immunological techniques. antisera prepared against each enzyme precipitate both glutamate dehydrogenases; upon immunodiffusion, the antiserum against the nuclear enzyme gives a line of incomplete identity with the two antigens, whereas the antiserum against the mitochondrial enzyme gives a line of complete identity. fractionation of the antibodies contained in each a ... | 1975 | 52373 |
| effects of proteins on thermotropic phase transitions of phospholipid membranes. | a variety of proteins have been studied for their ability to interact and alter the thermotropic properties of phospholipid bilayer membranes as detected by differential scanning calorimeter. the proteins studied included: basic myelin protein (a1 protein), cytochrome c, major apoprotein of myelin proteolipid (n-2 apoprotein), gramicidin a, polylysine, ribonuclease and hemoglobin. the lipids used for the interactions were dipalmitoylphosphatidylcholine and dipalmitoylphosphatidylglycerol. the in ... | 1975 | 52374 |
| quantitative immunocytochemistry of pituitary receptors for luteinizing hormone-releasing hormone. | in araldite sections of male rat pituitaries, stained after embedding by the unlabeled antibody enzyme method with antisera to native luteinizing hormone-releasing hormone (lh-rh) or lh-rh azo-conjugated to bovine serum albumin, localization is confined mainly to the interior of the large, and to a lesser extent to that of the small, secretion granules of the gonadotrophic cells. plasma membranes are not demonstrated. except for weak staining in the granules of corticotrophs, no other pituitary ... | 1975 | 52407 |
| desensitization of delayed-type hypersensitivity by antigen and specific antibody. | | 1975 | 52409 |
| on the nature of the external surface of cultured human embryo fibroblasts: an ultrastructural and cytochemical analysis utilising stain and lectin probes. | surfaces of human embryo fibroblasts in vitro were reacted with stain and lectin probes for carbohydrate moeities either after or in the absence of treatment with low concentrations of surface-active enzymes and edta. only testicular hyaluronidase significantly suppressed affinity-binding of all three agents, suggesting that acidic glycosaminoglycans are principle components of the cell's exterior. | 1975 | 52567 |
| a new abnormality of platelet functions. association of storage pool disease (thrombocytopathia a) with impaired reactivity of platelets to collagen. | multiple platelet abnormalities were found in a patient with bleeding symptoms. the platelet content of adp and pf 4 was decreased and the uptake of 14c-serotonin was impaired. the content of acid phosphatase, beta-glucuronidase and beta-n-acetylglucosaminidase was, however, normal and these enzymes were normally released or made available by bovine fibrinogen or adp. there was no adhesion of platelet to collagen, which also failed to induce reptilase clot retraction, platelet aggregation and re ... | 1975 | 52581 |
| [methodological studies on the immunologic determination of proconvertin (factor vii)]. | the influence of the different determinations of factor vii activities on the determination of the concentrations of the immunoreactive factor vii in the neutralizing test according to good-night were re-examined. the measurement of activity was performed with seitz filtered cattle plasma as a complex proof of factor vii and x and with a specific artificial deficiency plasma. the examinations had the following results: in the plasma of healthy grown-up people there is a considerable, apparently ... | 1975 | 52582 |
| conformational stability of antigenic determinant sites of bovine liver catalase. | | 1975 | 52593 |
| the immunological properties of pyruvate kinase. i: mammalian erythrocyte enzymes. | | 1975 | 52595 |
| antigen-induced inhibition of experimental allergic encephalomyelitis. iv. studies of the c-terminal end of the myelin basic protein molecule (1). | the c-terminal end of the myelin basic protein (bp) molecule (peptide 117.170) was obtained by cleavage with bnps-skatole, and purified by gel filtration. peptide 117-170 from guinea pig bp induced experimental allergic encephalomyelitis (eae) in lewis rats, whereas the corresponding peptide from bovine bp was inactive. neither peptide showed more than trace activity when tested in guinea pigs. guinea pigs and rats treated with peptide 117-170 in incomplete freund adjuvant were not rendered unre ... | 1975 | 52611 |
| specificity of the antibody response in inbred mice to bovine type i and type ii collagen. | mouse antibodies to soluble bovine skin (type i) collagen react with determinants which are located in the rigid triple-helical portion of the antigen and become destroyed upon unfolding the molecule. helical antigenic determinants are dependent on the genuine chain assembly, e.g. alpha[1(i)]2alpha2. artefactual triplehelical structures of the composition [alpha1(i)]3 or [alpha2]3 or a genetically distinct type ii collagen from cartilage showed no or only weak cross-reactivity. pepsin treatment ... | 1975 | 52615 |
| the potentiality of antibody-producing cells. ii. evidence for two antibody molecules of different specificities secreted by micromanipulated bispecific mouse spleen cells. | bispecific pfc appearing on the 4th day after immunization of mice with sheep erythrocytes conjugated with trinitrophenyl hapten (tnp-srbc) were identified by their capacity to lyse native srbc and tnp-conjugated horse erythrocytes (tnp--horbc) simultaneously in an open carboxymethylcellulose medium. individual pfc thus detected, were micromanipulated into two successive media containing the indicators srbc and tnp--horbc. out of 103 transferred double cells ninety-two (89 per cent) remained dou ... | 1975 | 52617 |
| cell co-operation and hapten--carrier complexes. | the co-operation of spleen cells of carrier- and hapten--carrier-primed mice in antibody formation against the hapten part of complexes was studied in 550 rad whole body irradiated mice. hapten--carrier complexes were prepared with the 2,4-dinitrophenyl group (dnp) as a hapten and heterologous bovine serum albumin (bsa) and isologous mouse immunoglobulin (mig) as carriers. priming of donor mice with carrier alone did not prepare for a secondary (igg) response in the recipients of hapten--carrier ... | 1975 | 52618 |
| immune complexes in the spleen. replacement of immune complexes trapped in spleen follicles by new immune complexes from the circulation. | the fate of intravenously injected 125i-bgg-anti-bgg in the spleen of mice was studied using autoradiography. part of the labelled immune complexes was trapped in the follicles of the spleen as could be expected. in a first experiment it was found that injections with unlabelled immune complexes were followed by a partial release of the labelled immune complexes from the follicles. in a second experiment unlabelled immune complexes retained in spleen follicles appeared to inhibit the trapping of ... | 1975 | 52623 |
| pre - proparathyroid hormone identified by cell - free translation of messenger rna from hyperplastic human parathyroid tissue. | an 8-15s fraction of rna isolated from hyperplastic human parathyroid tissue (primary chief-cell hyperplasia) and translated in a cell-free extract of wheat germ directs the synthesis of a protein that shares antigenic determinants and tryptic peptides with parathyroid hormone and its previously recognized immediated precursor, proparathyroid hormone. in addition, the protein contains tryptic peptides not found in proparathyroid hormone and migrates more slowly than does proparathyroid hormone o ... | 1975 | 52656 |
| examination of cottage cheese whey proteins by scanning electron microscopy: relationship to membrane fouling during ultrafiltration. | deposits formed on the membrane during ultrafiltration of aqueous solutions of individual whey proteins were examined under a scanning electron microscope. gamma-globulin formed granules which agglomerated and stacked into layers to form a porous matrix on the membrane. beta-lactoglobulin and bovine serum albumin both formed sheets on the membrane and formed multimers which were identified by sodium dodecyl sulfate gel electrophoresis. beta-lactoglobulin also formed strands where deposits were n ... | 1975 | 52661 |
| the effect of alloantisera on antigen-induced t cell proliferation. | in the guinea pig, alloantisera raised by cross-immunization of strain 2 and strain 13 animals are capable of specifically inhibiting the in vitro proliferative response of (2 x 13)f1 t lymphocytes to those antigens the response to which is controlled by ir genes linked to the genes controlling the alloantigens against which the serum is directed. however, in similar studies performed in the two parental strains, the responses to antigens not known to be under unigenic control were also markedly ... | 1975 | 52669 |
| determinants of the hierarchy of humoral immune responsiveness during ontogeny. | a model system of ontogeny was utilized to investigate the development of humoral immunity in both akr and balb/c mice. lethally irradiated adult mice were reconstituted with syngeneic fetal or neonatal liver. these mice were immunized at various times after reconstitution with a series of eight antigens: the bacteriophages f2, phix-174, and t4; the hapten carrier complexes 2,4 dinitrophenyl-bovine serum albumin and fluorescein-bovine serum albumin; and the small proteins: hen egg lysozyme, sper ... | 1975 | 52670 |
| fluorocarbon aerosol propellants. vii: interaction studies with human and bovine globulins using partition coefficient method. | | 1975 | 52706 |
| restoration of in-vitro lymphocyte responses with exogenous adenosine deaminase in a patient with severe combined immunodeficiency. | deficiency of adenosine deaminase (a.d.a.) occurs in an autosomal recessive form of severe combined immunodeficiency (s.c.i.d.). the role of this enzyme deficiency in the pathogenesis of the immune defects is not clear. a patient with a.d.a. s.c.i.d., studied during the first six weeks of life, was found to have b and t lymphocytes as well as 25% of normal lymphocyte responses to mitogens. this patient subsequently became severely lymphopenic with loss of mitogen responsiveness. addition of calf ... | 1975 | 52774 |
| letter: ultrasound and mammalian d.n.a. | | 1975 | 52798 |
| glycosaminoglycans from ateroid and bovine duodenal mucosa and pancreas. | glycosaminoglycans (gg) were isolated from commercial ateroid and compared with those from bovine duodenal mucosa and pancreas. the major gg in ateroid is heparin. heparan sulfate (hs) and dermatan sulfate were also found. hs, chondroitin sulfates, and heparin were isolated from duodenal mucosa after papain digestion, but a residue, non-digestible, was mostly heparin. pancreas contains very little gg, and the gg composition is similar to that of mucosa. the heparin isolated from ateroid and muco ... | 1975 | 52831 |
| biochemical approach to bovine leukemia. | many bovine leukemic lymphocytes produce virus particles when kept in survival cultures in eagle's minimum essential medium supplemented with 20 percent of inactivated fetal calf serum. virus particles equilibrate at a density of 1.16 g/ml in sucrose gradients and at a density of 1.12 g/ml in metrizamide gradients. simultaneous detection tests show that a high molecular weight rna-reverse transcriptase complex exists in these viruses. hybridizations between total rna from bovine leukemic lymphoc ... | 1975 | 52980 |
| hepatitis b surface antigen and human serum proteins. | the purification of hbs ag is briefly discussed with emphasis on the immunochemical characterization of the resulting preparations. results of experimental work in various laboratories aimed at establishing presence or absence of human serum proteins as integral parts of (highly purified) hbs ag particles do not yet allow definite conclusions. | 1975 | 53009 |
| regulation of the secondary antibody response in vitro. iii.--inhibitor-containing cells of antigen stimulated rabbit lymph node organ cultures. | the existence of inhibitor cells influencing the immune response has been inferred in many recent studies employing mice or chickens. the mechanism of this presumed suppression is unknown. the present report describes the isolation of aim (antibody inhibitory material) from migrant lymphoid cells which normally escape from fragements of rabbit lymph mode cultures. the rnase activity of aim was demonstrated and the implications of this property discussed. | 1975 | 53026 |
| in vitro culture of the proximal tubule of the bovine nephron : the fate of the histiospecific antigens and neosynthesis of an alpha-foetoprotein. | the isolation of pure tubules of the outer cortex of the foetal bovine kidney, using a previously described methods, has shown that the tubule suspension obtained is almost entirely constituted of proximal tubule fragments. the proximal cells obtained from this suspension were grown in vitro. the previously called "nephrospecific" antigens ns1-2 were shown to be strictly specific for the cells of the proximal tubule, which is an "histion" of the kidney, that is to say an ultimate unit of differe ... | 1975 | 53029 |
| activation of latent bovine gingival collagenase. | | 1975 | 53046 |
| effects of chymotrypsin and trypsin on rat peritoneal mast cells. | | 1975 | 53052 |
| a simple two-step isolation procedure for human and bovine antithrombin ii/iii (heparin cofactor): a comparison of two methods. | human and bovine antithrombin ii/iii have been isolated by a simple procedure essentially using only affinity chromatography on heparin-agarose and polyethylene glycol precipitation. an additional ammonium sulphate step removes polyethylene glycol and trace contaminants. the final preparation is homogeneous by sds-, disc- and agar slide electrophoresis. end group analysis of human antithrombin ii/iii shows histidine as the n-terminal amino acid. the pure preparation showed both progressive antit ... | 1975 | 53066 |
| retrograde axonal transport of exogenous protein in from nerves. | | 1975 | 53089 |
| effect of pretreatment with local corticosteroids on antibody-forming cells in the eye and draining lymph nodes. | to study the effect of pretreatment of rabbits with locally administered steroids, unilateral subconjunctival injections of 10 mg. triamcinolone suspension were given 10 and 3 days before intracorneal injection of the same eye with bovine gamma globulin. we killed the rabbits 6, 9, 12, 15, and 21 days after the bgg injection, and determined the number of antibody-producing cells in the homolateral lymph nodes, corneas, and uveal tracts. corticosteroids did not significantly affect the pfc respon ... | 1975 | 53091 |
| identification of the vasopressin producing and of the oxytocin producing neurons in the hypothalamic magnocellular neurosecretroy system of the rat. | immuno-enzyme histochemical investigations showed that, in the magnocellular hypothalamo-hypophysial neurosecretory system of the rat, vasopressin and oxytocin are synthetized in separate neurons. both the vasopressin neurons and the oxytocin neurons are present in both the supraoptic and the paraventricular nuclei in about the same number. preferential location of the two kinds of rat neurosecretory neurons is not as obvious as in the bovine hypothalamus. their perikarya do not show distinct mo ... | 1975 | 53102 |
| visualization of nucleolar organizer regions im mammalian chromosomes using silver staining. | a simple ammoniacal silver staining procedure, designated ag-as, differentially stains the chromosomal locations of ribosomal dna in certain mammalian species. this was critically demonstrated by ag-as staining of the nucleolus organizer regions in karyotypes of the same species and cell lines used for locating the ribosomal cistrons by dna/rna in situ hybridization. with ag-as, silver stained nors (ag-nors) are visualized as black spherical bodies on yellow-brown chromosome arms. ag-nors were v ... | 1975 | 53131 |
| recent status of organochlorine pesticide residues in foods in japan. | in japan, organochlorine compounds, especially bhc, were used in large quantities. consequently, their accumulation in the human body or in live-stock through feed crops, and the contamination of mother's and cow's milk caused serious public concern. this prompted not only initial stricter regulation of their use but the eventual prohibition of bhc and ddt use in 1971. fortunately, the contamination of mother's and cow's milk is decreasing by these steps. the japanese daily bhc intake was 0.57, ... | 1975 | 53149 |
| defense mechanisms against bovine herpesvirus: relationship of virus-host cell events to susceptibility to antibody-complement cell lysis. | the interaction of infectious bovine rhinotracheitis virus and susceptible host cells was examined to determine whether an infected cell could be destroyed by humoral immune mechanisms before or after the transmission of virus to susceptible adjacent cells. viral antigens were detectable on cell membranes at 6 h postinfection, but cells were not susceptible to antibody-complement lysis until 10 h postinfection. intracellular infectious virus was also detectable at 10 h postinfection, and transmi ... | 1975 | 53206 |
| selective and non-selective lymphocytotoxicity in human melanoma: observation on the effect of long-term culture and fetal bovine serum on target-cell sensitivity to lymphocytes. | in vitro cell mediated cytotoxicity (cmc) assays have been conducted in a human melanoma system with a 3h-proline retention technique. melanoma target cells from long-term cultures ("cell lines") are found to exhibit increased susceptibility for lymphocyte cytotoxicity in comparison to the same target cells from short-term culture. the higher sensitivity of the "cell line" derived target cells is seen with lymphocytes, irrespective of diagnosis of the donor. in parallel experiments with the targ ... | 1975 | 53212 |
| antigenic determinants of bovine myelin encephalitogenic protein recognized by rabbit antibody to myelin encephalitogenic protein. | the antigenic determinants of bovine myelin encephalitogenic protein were investigated by quantitative complement fixation and hapten inhibition using rabbit anti-monkey protein and anti-bovine protein and purified and characterized fragments of bovine protein. the two regions of bovine encephalitogenic protein containing determinants were sequences 1 to 43 and 90 to 170. one rabbit antiserum recognized a determinant(s) probably in residues 15 to 40 while for another rabbit antiserum the determi ... | 1975 | 53232 |
| mustard meal in dairy rations. | consumption of 0% mustard meal and 15% soybean meal, 7.5% mustard meal and 7.5% soybean meal, or 15% mustard meal and 0% soybean meal rations did not differ in palatability studies with 10 group-fed lactating cows when the mustard meal was treated with 3% caustic soda. order of preference was for 0, 7.5, and 15% mustard meal rations when mustard meal was untreated. twelve lactating cows were in each of two lactation trials to compare the three rations of untreated mustard meal. milk, milk fat, a ... | 1975 | 53243 |
| the intrinsic fluorescence characteristics of the myelin basic protein. | | 1975 | 53272 |
| the estimation of the proportion of motile bull spermatozoa in various diluents and a comparison with the proportion eosinophilic. | the proportion of motile spermatozoa in bull semen diluted with phosphate-buffered saline, citrate or milk has been estimated both photographically and with an image-analysing computer. the two methods produced similar estimates. the proportion of noneosihophilic spermatozoa was higher than the proportion of immotile spermatozoa, particularly when fluoride or formaldehyde was added to the diluents. the proportion of non-eosinophilic spermatozoa in diluents containing formaldehyde was the same as ... | 1975 | 53285 |
| preservation of differential staining of spermatozoa by formol citrate. | semen from boar, bull, ram, rabbit, reindeer and stallion was diluted in formol citrate or formol saline and stained with eosinnigrosin. the proportion of eosinophilic spermatozoa did not differ from that in fresh semen after storage for 48 hr in the formol diluent at temperatures ranging from 4 degrees c to 40 degrees c. some samples were kept for periods up to 3 weeks with very little increase in the proportion of eosinophilic spermatozoa. | 1975 | 53286 |
| mucosal adherence of human enteropathogenic escherichia coli. | an in-vitro assay system has been developed to measure bacterial adhesion to the mucosa of human fetal small intestine. two strains of escherichia coli that are proven human enteropathogens (e.p.e.c.) have been shown to adhere in large numbers, compared with control organisms. the attachment mechanism is species specific and is not caused by common fimbriae. mucosal adhesion may be as important as enterotoxin production or invasiveness in determining the virulence of some strains of human e.p.e. ... | 1975 | 53431 |
| editorial: polyunsaturated beef-eaters. | | 1975 | 53438 |
| letter: isolation of human rotavirus using human embryonic gut monolayers. | | 1975 | 53455 |
| recognition of self from non-self in crustaceans. | | 1975 | 53792 |
| heterologous gamma globulin as interferon inducer: differences in the properties of aggregated and aggregate-free gamma globulin preparations. | | 1975 | 53854 |
| a direct spectrometric method for determination of the concentration of available bilirubin binding sites in serum using bromphenol blue. | a simple and rapid direct spectrometric micromethod for the determination of the concentration of available bilirubin binding sites of serum is given. the method measures - by means of difference spectrometry - the maximal change in absorbance when serum is added to a buffered solution of bromphenol blue (bpb). this dye fulfills a set of requirements given and examined. the binding properties of bilirubin, bpb, and some related dyes are spectrometrically investigated, and differences between the ... | 1975 | 53876 |
| proceedings: activation of dicoumarol-induced atypical prothrombin. | | 1975 | 53896 |
| [skin disease in cattle, caused by dermatophilus congolensis]. | | 1975 | 53909 |
| [age changes in the composition of immunoglobulins in cattle]. | the electrophoretic and immunochemical methods as well as gelfiltration through sephadex g-200 showed that there are no immunoglobulins in blood serum of fetuses and new-born calves. specific proteins in a zone of gamma-globulins electrophoretic mobility which correspond to ig m and ig a are found in the serum of calves 3 hrs after their feeding on colostrum. the content of these proteins reaches the maximum on the third day of life but at the age of three months it lowers up to minimum. on the ... | 1975 | 53931 |
| the practice laboratory: the interpretation of blood smears. | | 1975 | 53937 |
| [the serum gamma-globulin picture in leucosis of cattle and sheep]. | | 1975 | 53970 |
| effect of encephalitogenic protein on the migration in agarose of leucocytes from patients with multiple sclerosis. correlations with clinical parameters, localization of antigenic determinants, and influence of corticotrophin treatment. | we have extended the results from our previous investigation of the reactivity of leucocytes from patients with multiple sclerosis and some other neurological diseases to bovine encephalitogenic protein, using the migration in agarose technique. 1. reactivity (i.e. behaviour in the test deviating from that expected with cells from healthy subjects) is manifested either as an enhancement or an inhibition of leucocyte migration. 2. the incidence of subjects with cells showing reactivity to encepha ... | 1975 | 53988 |
| [adsorption of polycyclic compound to uveal melanin granule (author's transl)]. | | 1975 | 53992 |
| agglutinability of cattle red cells. 4. the effect of antiglobulin in comparison with treatment by pronase. | the average titre scores varied from zero to 24.7 for the cattle red cells (crc) from different mz pairs. these crc were sensitized with different blood-typing reagents and were titre-tested against the double dilution series of an anti-bovine gamma-globulin serum. a significant negative correlation (r = - 0.82; p less than 0.001) was found between the degree of agglutinability and the amount of neuraminic acid of the surface component of crc cleaved by pronase. after pronase treatment of the cr ... | 1975 | 54009 |
| immunological and chemical correlation between alpha-fetoproteins from human and several mammalian species. | alpha-fetoproteins of several animals were purified and their molecular weights, amino acid compositions and peptide maps were compared, demonstrating the close similarities. these data indicated that the alpha-fetoproteins of mammalian species have closely related antigenical and chemical structures. rabbits and horses were immunized with human alpha-fetoprotein, and it was observed that the animals produced antibodies reaction not only with human alpha-fetoprotein but with their homologous alp ... | 1975 | 54014 |
| [proceedings: microsomal lysophospholipase in bovine thyroid gland]. | | 1975 | 54084 |
| [proceedings: lipid composition of bovine thyroid gland. iv. glycolipids]. | | 1975 | 54117 |
| proceedings: cardiac pace-maker currents and extracellular ph. | | 1975 | 54142 |
| natural antibodies against colicines in human and animal sera. | the presence of "natural antibodies" against colicines was detected in the sera of 13 (29.5%) hospitalized recruits aged 20-29 years, 4 (25%) hospitalized males aged 30-49 years, 8 (40%) outpatient elderly subjects aged 50-79 years and in the sera of 15 (27.2%) animals. it is discussed the possible role of colicines as antigenic determinants stimulating the production of "natural antibodies", because of the prolonged and repeated contact of colicinogenic strains with the host animals. | 1975 | 54185 |
| calf lens messenger ribonucleoprotein complexes. characterization and comparison of template activity with corresponding mrnas. | lens messenger ribonucleoprotein complexes have been isolated from calf lens polysomes by sucrose gradient centrifugation after puromycin-induced dissociation. a 10 s mrna was released from a 13 s messenger ribonucleoprotein complex and a 14 s mrna from a 19 s messenger ribonucleoprotein complex. two major protein components with molecular weights of approx. 64 000 and 40 000 were isolated from each of the messenger ribonucleoprotein complexes after rnaase digestion. buoyant density determinatio ... | 1976 | 54193 |
| e and eac rosetting lymphocytes in patients with carcinoma of bronchus. ii. a sequential study of thirty patients: effect of bcg. | t and b lymphocytes have been estimated in thirty patients with carcinoma of bronchus at monthly intervals. the patients have been treated by surgery, radiotherapy or no conventional antineoplastic therapy, with or without monthly intradermal bcg. significant increase in t-lymphocyte percentages occurred in three out of six patients treated by bcg and no conventional therapy, and similar increases were observed in other bcg-treated patients. patterns emerged suggesting that the t-lymphocyte perc ... | 1975 | 54235 |
| antigenic structure of the cyanogen bromide peptide f-cb3 from fibrinogen alpha-chain. | the antigenic properties of the cyanogen bromide peptide f-cb3 from bovine fibrinogen alpha-chain were studied in radioimmune assays with rabbit antibodies to fibrinogen or to peptide f-cb3. both fibrinogen and peptide f-cb3 were indistinguishable in inhibition and dissociation tests. modification of the single disulfide bridge in peptide f-cb3 either by reduction or by cleavage with cyanide was not accompanied by loss of serologic activity. inhibition studies with three individual fragments obt ... | 1975 | 54258 |
| bovine fibrinogen-induced 14c-serotonin and other compounds release from human blood platelets. | bovine fibrinogen can aggregate human blood platelets by a direct mechanism (first wave) and also indirectly by releasing endogenous adp (second wave). a primarily non-altered surface structure of the thrombocytes is important for the induction of the first wave. under certain conditions a second aggregation wave, concomitant with the release and the availability reaction is elicited. besides the cell's contact, the presence of adp and calcium ions are necessary for the induction of the release ... | 1975 | 54319 |
| anti-maleyl antibody. | | 1975 | 54330 |
| serological relationship of taxonomically closely related domestic ruminants: cattle, buffalo, sheep & goat. | | 1975 | 54334 |
| antigenic properties of polymers formed by beta-lactam antibiotics. | purified polymers have been isolated from 6-aminopenicillanic acid and from semi-synthetic penicillins and cephalosporins. all the polymers were shown to react with rabbit antibody of penicillins and cephalosporins. all the polymers were shown to react with rabbit antibody of penicilloyl specificity, as demonstrated by passive cutaneous anaphylaxis in guinea pigs, and the reactions were shown to be penicilloyl-specific by hapten inhibition experiments. the cephalosporin-derived polymers in addit ... | 1976 | 54343 |