| ptfe bypass or thrupass for superficial femoral artery occlusion? a randomised controlled trial. | early results of a thrupass endograft in the treatment of femoral lesions are promising. less morbidity and better cost-effectiveness are suggested to be achieved in the treatment of chronic lower limb ischaemia with endovascular treatment compared to surgical treatment. | 2009 | 19231250 |
| immobilization of oligonucleotides on titanium based materials by partial incorporation in anodic oxide layers. | this paper describes the immobilization of bioactive molecules on titanium based surfaces through a combination of nano-mechanical fixation of nucleic acid anchor strands (ass) by partial and regioselective incorporation within an anodic oxide layer and their hybridization with complementary strands (css) intended to be conjugated to bioactive molecules. we focus on the interaction between the substrate surface and the anchor strands, the integrity of ass and their hybridization ability. the obs ... | 2009 | 19232713 |
| invited review: proteomics of milk and bacteria used in fermented dairy products: from qualitative to quantitative advances. | proteomics is a powerful tool that can simultaneously analyze several hundred proteins in complex mixtures, either through the use of high-resolution 2-dimensional gel electrophoresis or by mono- and multi-dimensional liquid chromatography coupled with mass spectrometry. since the last review in 2005, proteomics has mainly been applied to describe minor proteins in the bovine milk fat globule membrane and soluble proteins in human colostrum. at least 130 new minor proteins have been identified. ... | 2009 | 19233774 |
| castration of horses under total intravenous anaesthesia: analgesic effects of lidocaine. | to evaluate the effects of local anaesthesia with lidocaine for castration of horses under intravenous anaesthesia. | 2009 | 19239656 |
| likelihood evaluation using 15 common short tandem repeat loci: a practical and simulated approach to establishing personal identification via sibling/parental assessments. | in individual identification, often the only way to circumvent the lack of personal reference materials is to resort to a kinship analysis between the unidentified party (up) and putative relatives. | 2009 | 19243547 |
| characteristics of interstitial lung disease in ss-a positive/jo-1 positive inflammatory myopathy patients. | the strongest predictive factor for the development of interstitial lung disease (ild) in myositis (iim) patients is the presence of different antisynthetase antibodies. the aim of this study was to compare the clinical characteristics, radiological findings and therapeutic response between the anti-ss-a positive and negative antisynthetase syndrome (ass) patients. a prospective study of 315 iim patients was conducted including 27 anti-jo-1 positive ass patients. mean disease duration was 46.6 ( ... | 2009 | 19266202 |
| glycan-binding profile of a d-galactose binding lectin purified from the annelid, perinereis nuntia ver. vallata. | a lectin recognizing d-galactose was purified from the pacific annelid perinereis nuntia ver. vallata (polychaeta) by affinity chromatography. hemagglutinating activity, with a very low titer suggesting the presence of lectin appeared in the supernatant from the homogenization of body with tris-buffered saline. however, dialyzed supernatant from the precipitate homogenized by galactose in the buffer revealed strong hemagglutinating activity against human erythrocytes. the crude supernatant was a ... | 2009 | 19266618 |
| [the role of lipid peroxidation in pathogenesis of hepatic enzymatic dysfunction during peritoneal intoxication]. | we have investigated the role of lipid per oxidation in pathogenesis of hepatic enzymatic dysfunction during peritoneal intoxication. the investigations were carried out on 36 albino rats. i group were included intact animals, ii group were included animals that peritoneal exudates reached with products of lipid per oxidation were injected to abdominal cavity and iii group were included reached with animals that superoxiddismutase together with products of lipid per oxidation were injected to ab ... | 2009 | 19276481 |
| adi, autophagy and apoptosis: metabolic stress as a therapeutic option for prostate cancer. | prostate cancer, the leading incidence of cancer in american males, is a disease in which treatment of nonlocalized tumors remains largely unsuccessful. these cancers lose expression of an arginine synthesis enzyme, argininosuccinate synthetase (ass), and are susceptible to arginine deprivation by arginine deiminase (adi). we show cwr22rv1 prostate cancer cells are susceptible to adi in a caspase-independent manner in vitro and in a xenograft model in vivo. we demonstrate that single amino acid ... | 2009 | 19276647 |
| genetic and epidemiological characterization of stretch lagoon orbivirus, a novel orbivirus isolated from culex and aedes mosquitoes in northern australia. | stretch lagoon orbivirus (slov) was isolated in 2002 from pooled culex annulirostris mosquitoes collected at stretch lagoon, near the wolfe creek national park in the kimberley region of western australia. conventional serological tests were unable to identify the isolate, and electron microscopy indicated a virus of the genus orbivirus, family reoviridae. here, a cdna subtraction method was used to obtain approximately one-third of the viral genome, and further sequencing was performed to compl ... | 2009 | 19282430 |
| molecular profiles of venezuelan isolates of trypanosoma sp. by random amplified polymorphic dna method. | nine trypanosoma sp. venezuelan isolates, initially presumed to be t. evansi, were collected from three different hosts, capybara (apure state), horse (apure state) and donkey (guarico state) and compared by the random amplification polymorphic dna technique (rapd). thirty-one to 46 reproducible fragments were obtained with 12 of the 40 primers that were used. most of the primers detected molecular profiles with few polymorphisms between the seven horse, capybara and donkey isolates. quantitativ ... | 2009 | 19286320 |
| a molecular survey of piroplasmida and hepatozoon isolated from domestic and wild animals in burgos (northern spain). | this study reports a molecular survey of hepatozoon species and of the order piroplasmida in the province of burgos, northern spain. the diagnostic techniques employed included pcr and the sequencing of the 18s rrna gene. eighty-nine blood samples from domestic animals plus 138 blood/coagulated blood samples from wild mammals were examined. theilerid protozoa were found at relatively high frequencies in bovines (14.6%) and horses (36%). theileria buffeli, t. sergenti and t. annulata were diagnos ... | 2009 | 19297099 |
| acute splenic sequestration in a cohort of children with sickle cell anemia. | to analyze acute splenic sequestration (ass) in children with sickle cell anemia diagnosed through a newborn screening program in the state of minas gerais, brazil, and followed up at the hematology center in the city of belo horizonte, minas gerais, brazil. | 2009 | 19319450 |
| characterization of hypertriglyceridemia and response to treatment with insulin in horses, ponies, and donkeys: 44 cases (1995-2005). | to characterize signalment, clinical signs of disease, and clinical response to insulin in equids with hypertriglyceridemia. | 2009 | 19335242 |
| acidities of arsenic (iii) and arsenic (v) thio- and oxyacids in aqueous solution using the cbs-qb3/cpcm method. | a number of calculations of acidic as(iii) and as(v) species formed with sulfur and oxygen (h(3)ass(3), h(2)ass(3)(-), hass(3)(2-), ass(3)(3-), h(3)ass(4), h(2)ass(4)(-), hass(4)(2-), ass(4)(3-), h(3)aso(3), h(2)aso(3)(-), haso(3)(2-), aso(3)(3-), h(3)aso(4), h(2)aso(4)(-), haso(4)(2-), and aso(4)(3-)) are presented. pk(a)s for successive deprotonations in both the gas phase and aqueous solution (using both explicit water molecules and a self-consistent reactive field conductive polarizable cont ... | 2009 | 19351160 |
| a perspective on theileria equi infections in donkeys. | the donkey population has remained unchanged in the last two decades despite a decrease in the overall population of equids, emphasizing the usefulness of the donkey as a draught and pack animal. piroplasmosis in donkeys, caused by theileria equi and babesia caballi, has been recognized as a serious problem of major economic importance as the affected animals manifest decreased working capacity, loss of appetite, etc. in tropical countries, t. equi infections are more wide-spread and pathogenic ... | 2009 | 19358444 |
| citrullinemia type i, classical variant. identification of ass-p~g390r (c.1168g>a) mutation in families of a limited geographic area of argentina: a possible population cluster. | citrullinemia type i (ctln1) is an urea cycle defect caused by mutations in the argininosuccinate synthetase gene. we report the first identification in argentina of patients with ctln1 in a limited geographic area. | 2009 | 19358837 |
| verocytotoxigenic escherichia coli o157 in animals on public amenity premises in england and wales, 1997 to 2007. | at the request of the public health authorities, 31 public amenity premises in england and wales containing animals of various species were investigated for the presence of verocytotoxigenic escherichia coli (vtec) o157 between 1997 and 2007, because of putative associations with human cases. vtec o157 was confirmed in one or more species on 19 (61.3 per cent) of the premises. there were significant associations between the presence of vtec o157 and the number of species sampled, the size of the ... | 2009 | 19411683 |
| surgical removal of an aural sarcoid in a donkey using ultrasonic shears. | | 2009 | 19411689 |
| electrocardiographic parameters in the clinically healthy zamorano-leones donkey. | limited information exists regarding electrocardiographic parameters in clinically healthy donkeys. the study was carried out in 75 healthy adult animals (40 females and 35 males) using the einthoven standard ii and base-apex leads. the p wave showed usually a bifid shape deflection. the qrs complex of the donkeys appeared in several forms: qr and r were the most frequent in limb lead ii, and qs and qr in the base-apex lead. most t waves presented a simple negative configuration in lead ii and b ... | 2009 | 19433331 |
| pcr detection of african horse sickness virus serogroup based on genome segment three sequence analysis. | a nested reverse transcriptase (rt) polymerase chain reaction (rt-pcr), for rapid detection of african horse sickness virus (ahsv) double-stranded ribonucleic acid (dsrna) in cell culture and tissue samples, was developed and evaluated. using an outer pair of primers (p1 and p2), selected from genome segment three of ahsv serotype 6 (ahsv-6), the rt-pcr-based assay resulted in amplification of a 890 base pair (bp) primary pcr product. rnas from the nine vaccine strains of ahsv, and a number of a ... | 2009 | 19442836 |
| sequence and phosphorylation level determination of two donkey beta-caseins by mass spectrometry. | two coeluting components, with experimentally measured m(r) values of 25529 and 24606 da, were identified by reversed-phase high-performance liquid chromatography (rp-hplc) and mass spectrometric analysis in the dephosphorylated casein fraction of a milk sample collected from an individual donkey belonging to the ragusano breed of the east of sicily. by coupling enzymatic digestions, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) and rp-hplc/nano-elec ... | 2009 | 19462407 |
| thirty-two component finite element models of a horse and donkey digit. | the finite element (fe) method is the most powerful modelling technique available to explicate the biomechanics of the digit. it has already proved to be of high value in human podiatry. however, accurate models of the complex anatomy of the horse and donkey digit are currently lacking. | 2009 | 19469224 |
| dimensions of diastemata and associated periodontal food pockets in donkey cheek teeth. | equine cheek teeth (ct) diastemata often cause deep periodontal food pocketing and are therefore regarded as a painful dental disorder of equidae. however there appears to be no information available on the size or shape of these diastemata. this post mortem study examined 16 donkey skulls (mean age = 32-years) containing 45 ct diastemata to define the anatomical shape and dimensions of these diastemata, and of the associated periodontal food pockets that occur with this disorder. diastemata wer ... | 2009 | 19476082 |
| latherin: a surfactant protein of horse sweat and saliva. | horses are unusual in producing protein-rich sweat for thermoregulation, a major component of which is latherin, a highly surface-active, non-glycosylated protein. the amino acid sequence of latherin, determined from cdna analysis, is highly conserved across four geographically dispersed equid species (horse, zebra, onager, ass), and is similar to a family of proteins only found previously in the oral cavity and associated tissues of mammals. latherin produces a significant reduction in water su ... | 2009 | 19478940 |
| real-time molecular recognition between protein and photosensitizer of photodynamic therapy by quartz crystal microbalance sensor. | real-time investigation of molecular recognition between protein and the photosensitizer of photodynamic therapy (pdt) was carried out by a quartz crystal microbalance (qcm) sensor integrated into a flow injection analysis (fia) system. the photosensitizer meso-tetrakis(4-hydroxyphenyl)porphyrin (p-thpp) was immobilized on the gold electrode of the qcm chip by combining the sol-gel and self-assembly methods. such a rapid screen analysis of molecular recognition showed that the p-thpp-immobilized ... | 2009 | 19481051 |
| s100b milk concentration in mammalian species. | s100b is a neurotrophic protein detectable in biological fluids and in human milk. since there are several maternal-neonatal conditions requiring the administration of animal milks the aim of the present study was to quantify s100b in milk from different mammalian species and to compare protein's concentration among human and mammalian milks. we assessed s100b concentrations in donkey (n=12), goat (n=15) sheep (n=15), commercially available cow (n=8) and human (n=15) milk samples. s100b measurem ... | 2009 | 19482669 |
| argininosuccinate synthetase is a functional target for a snake venom anti-hypertensive peptide: role in arginine and nitric oxide production. | bj-bpp-10c is a bioactive proline-rich decapeptide, part of the c-type natriuretic peptide precursor, expressed in the brain and in the venom gland of bothrops jararaca. we recently showed that bj-bpp-10c displays a strong, sustained anti-hypertensive effect in spontaneous hypertensive rats (shr), without causing any effect in normotensive rats, by a pharmacological effect independent of angiotensin-converting enzyme inhibition. therefore, we hypothesized that another mechanism should be involve ... | 2009 | 19491403 |
| paediatric open tibiofibular fractures following a donkey bite. a report of two cases. | open tibiofibular fractures resulting from domestic animal bites are exceptional. we report two such cases, in patients of 11 and 13 years of age, and discuss the etiological, pathogenic, anatomic, and clinical aspects of these injuries. these were type iii fractures according to the cauchoix and duparc classification. the fractures were treated with emergency wound care, wound dressing, and conservative orthopaedic treatment using a long leg cast for immobilization. preventive parenteral antibi ... | 2009 | 19502122 |
| [percutaneous radiofrequency ablation of osteoid osteomas: technique and results]. | osteoid osteoma is a benign primary bone tumor that typically occurs in children and young adults. besides local pain, which is often worse at night, prompt relief due to medication with acetylsalicylic acid (ass) is characteristic for this bone lesion. because long-term medication with ass does not represent an alternative treatment strategy due to its potentially severe side effects, different minimally invasive image-guided techniques for the therapy of osteoid osteoma have been developed. in ... | 2009 | 19517335 |
| trypanosoma vivax displays a clonal population structure. | african animal trypanosomiasis, or nagana, is a debilitating and economically costly disease with a major impact on animal health in sub-saharan africa. trypanosoma vivax, one of the principal trypanosome species responsible for the disease, infects a wide host range including cattle, goats, horses and donkeys and is transmitted both cyclically by tsetse flies and mechanically by other biting flies, resulting in a distribution covering large swathes of south america and much of sub-saharan afric ... | 2009 | 19520081 |
| cu(ii) bis(thiosemicarbazone) radiopharmaceutical binding to serum albumin: further definition of species dependence and associated substituent effects. | the pyruvaldehyde bis(n(4)-methylthiosemicarbazonato)copper(ii) (cu-ptsm) and diacetyl bis(n(4)-methylthiosemicarbazonato)copper(ii) (cu-atsm) radiopharmaceuticals exhibit strong, species-dependent binding to the iia site of human serum albumin (hsa), while the related ethylglyoxal bis(thiosemicarbazonato)copper(ii) (cu-ets) radiopharmaceutical appears to exhibit only nonspecific binding to hsa and animal serum albumins. | 2009 | 19520290 |
| rituximab treatment of the anti-synthetase syndrome: a retrospective case series. | interstitial lung disease (ild) is the major determinant of morbidity and mortality in the anti-synthetase syndrome (ass). here we have retrospectively assessed 11 ass patients with ild treated with the anti-cd20 mab rituximab at our tertiary referral hospital. | 2009 | 19531628 |
| [diagnosis and treatment of preeclampsia: overview of international recommendations]. | the new german guidelines for the treatment of hypertensive disorders in pregnancy have some important differentiations. the aim of the new guidelines is to avoid the early premature birth and to reduce the fetal and maternal morbidity and mortality. for the first time there ist the possibility and the recommendation for the prolongation of pregnancy. this means that the treatment of hypertensive disorders should start not before a blood pressure of >or=170/110 mmhg; thus the nutrition of the fe ... | 2009 | 19536711 |
| gastrointestinal parasites of working donkeys of ethiopia. | the general prevalence and population composition of gastrointestinal and pulmonary helminths of working donkeys were studied. for the purpose 2935 working donkeys were coprologically examined for nematode and cestode, and 215 donkeys for trematode infections. seven donkeys that died due to various health problems or were euthanased on a welfare ground were necropsied and the parasites were recovered and identified to the species level. the study was conducted during the periods 1996-1999.coprol ... | 2010 | 19548106 |
| light and scanning electron microscopic study on the tongue and lingual papillae of the common hippopotamus, hippopotamus amphibius amphibius. | we observed the three-dimensional structures of the external surface and connective tissue cores ctcs, after exfoliation of the epithelium of the lingual papillae (filiform, fungiform, and foliate papillae) of the common hippopotamus (hippopotamus amphibius amphibius) using scanning electron microscopy and conventional light microscopy. following unique features were found; typical vallate papillae with a circumferential furrow were not observable. instead, numerous large fungiform papillae were ... | 2009 | 19548302 |
| epidemiology of animal bites in rafsanjan, southeast of islamic republic of iran, 2003-05. | the incidence of animal bites in the islamic republic of iran has been increasing in recent years. in a descriptive study of 1542 patients referred to a health centre in rafsanjan city from 2003 to 2005, the incidence of animal bites in the area was estimated as 180, 195 and 241 per 100 000 in 2003, 2004 and 2005 respectively. of the patients, 74% were bitten by dogs, 23% by cats and 3% by other animals (monkeys, rats, foxes, bats, donkeys, etc.). the mean time delay from injury to initial manag ... | 2009 | 19554994 |
| clinical dental examinations of 357 donkeys in the uk. part 1: prevalence of dental disorders. | dental disorders have a high prevalence in older donkeys and horses, but the nature and pathogenesis of many of these disorders have yet to be established. | 2009 | 19562902 |
| clinical dental examinations of 357 donkeys in the uk. part 2: epidemiological studies on the potential relationships between different dental disorders, and between dental disease and systemic disorders. | dental disease has been shown to be a risk factor for weight loss and colic in horses. no extensive clinical studies in donkeys have investigated the potential relationship between different dental disorders, or between dental disease and systemic disorders. | 2009 | 19562903 |
| [study on the pathogen of plague in sanjiangyuan area in qinghai province]. | to study the biological characteristics of yersinia pestis and to develop prevention and control program on plague in sanjiangyuan areas, qinghai province. | 2009 | 19565850 |
| nmr structural analysis of the soluble domain of ziaa-atpase and the basis of selective interactions with copper metallochaperone atx1. | a cu(i) metallochaperone, atx1, interacts with the amino-terminal domain of a cu(i)-transporting atpase, pacs(n), but not with a domain of related zn-transporting atpase, ziaa(n) in synechocystis pcc 6803. this is thought to prevent ziaa(n) from acquiring cu(i), which it binds more tightly than zn. solution structures of atx1, pacs(n), and the heterodimer were previously described. here we report solution structural studies of the ziaa(n) soluble domain. apo-ziaa(n) has a typical ferredoxin-like ... | 2010 | 19609573 |
| effects of crowding on ornithine-urea cycle enzyme mrna expression and activity in gulf toadfish (opsanus beta). | the gulf toadfish (opsanus beta) is a facultatively ureotelic fish that excretes primarily urea under conditions of crowding or confinement. to examine the relationship between ammonia production, urea production and the ornithine-urea cycle (o-uc) enzyme activity and mrna expression, we subjected toadfish to two-day and seven-day crowding regimes. plasma cortisol levels were measured and liver tissue was assayed for ammonia and urea concentrations. liver glutamine synthetase (gs), carbamoyl pho ... | 2009 | 19617432 |
| cdse/ass core-shell quantum dots: preparation and two-photon fluorescence. | arsenic(ii) sulfide (ass)-coated cdse core-shell nanocrystals can be prepared by a cluster-complex deposition approach under mild conditions. at 60 degrees c, growth of an ass shell onto a cdse nanocrystal can be realized through the crystallization of a cluster complex of ass/butylamine in a mixed solvent of isopropanol/chloroform. the new, type i core-shell nanocrystal exhibits markedly enhanced one-photon fluorescence as well two-photon upconversion fluorescence. the nanocrystals can be used ... | 2009 | 19627085 |
| neural correlates of decision making on a gambling task. | individual differences in affective decision making were examined by recording event-related potentials (erps) while 74 typically developing 8-year-olds (38 boys, 36 girls) completed a 4-choice gambling task (hungry donkey task; e. a. crone & m. w. van der molen, 2004). erp results indicated: (a) a robust p300 component in response to feedback (punishment vs. reward outcomes), (b) anticipation effects (stimulus-preceding negativity) prior to outcomes presented on frequent (vs. infrequent) punish ... | 2009 | 19630895 |
| neural stem cells improve cognition via bdnf in a transgenic model of alzheimer disease. | neural stem cell (nsc) transplantation represents an unexplored approach for treating neurodegenerative disorders associated with cognitive decline such as alzheimer disease (ad). here, we used aged triple transgenic mice (3xtg-ad) that express pathogenic forms of amyloid precursor protein, presenilin, and tau to investigate the effect of neural stem cell transplantation on ad-related neuropathology and cognitive dysfunction. interestingly, despite widespread and established ass plaque and neuro ... | 2009 | 19633196 |
| re-emergence of west nile virus in italy. | in august 2008, west nile disease re-emerged in italy. the infection is affecting the north eastern regions and, as of november 2008, has caused 33 clinical cases and five fatalities in horses. until now, no deaths have been reported in birds. mosquitoes, blood, serum and tissue samples, from horses and birds, within and around the outbreak area, have been collected and tested by various methods both serologically and virologically. west nile virus strains have been isolated from blood samples o ... | 2010 | 19638165 |
| diverse modes of 5'-[4-(aminoiminomethyl)phenyl]-[2,2'-bifuran]-5-carboximidamide (db832) interaction with multi-stranded dna structures. | the modes of binding of 5'-[4-(aminoiminomethyl)phenyl]-[2,2'-bifuran]-5-carboximidamide (db832) to multi-stranded dnas: human telomere quadruplex, monomolecular r-triplex, pyr/pur/pyr triplex consisting of 12 t*(t x a) triplets, and dna double helical hairpin were studied. the optical adsorption of the ligand was used for monitoring the binding and for determination of the association constants and the numbers of binding sites. cd spectra of db832 complexes with the oligonucleotides and the dat ... | 2010 | 19642208 |
| synthesis and characterization of one-dimensional and molecular m(tren)inass4 (m = mn, co, and zn) compounds with a noncondensed ass3(3-) unit. | four transition-metal-amine complexes incorporating indium thioarsenates with the general formula m(tren)inass(4) (m = mn, co, and zn) and a noncondensed ass(3)(3-) unit have been prepared and characterized. single-crystal x-ray diffraction analyses show that compound 1 (m = mn) crystallizes in the triclinic crystal system (space group: p1) and consists of a one-dimensional (1d) inorganic (1)(infinity){[inass(4)](2-)} chain and [mn(tren)](2+) groups bonded to the opposite sides of an eight-membe ... | 2009 | 19645431 |
| neutralization of bothrops mattogrossensis snake venom from bolivia: experimental evaluation of llama and donkey antivenoms produced by caprylic acid precipitation. | polyspecific bothropic/crotalic and bothropic/lachesic antivenoms were produced in bolivia by immunizing two donkeys with the venoms of bothrops mattogrossensis and crotalus durissus terrificus and one llama with the venoms of b. mattogrossensis and lachesis muta. these antivenoms are currently being used for snakebite envenomation in bolivia. the rationale for using these animals is that donkeys and llamas are better adapted than horses to the high altitudes in south america and constitute good ... | 2010 | 19647761 |
| [ass plus clopidogrel in contraindication for marcumar]. | | 2009 | 19658286 |
| sporotrichosis: a retrospective evaluation of 23 cases seen in northern california (1987-2007). | sporotrichosis is an uncommon to rare cutaneous and subcutaneous mycosis of animals and humans caused by the dimorphic fungus sporothrix schenckii. twenty-three mammalian cases of sporotrichosis examined between 1987 and 2007 at the university of california, davis - veterinary medical teaching hospital, were retrospectively evaluated with regard to the historical, clinical, diagnostic and treatment findings. cats were the most common species affected (n = 14). in addition, sporotrichosis was dia ... | 2009 | 19659536 |
| inhibition of human initiator caspase 8 and effector caspase 3 by cross-class inhibitory bovserpina3-1 and a3-3. | serpins are a superfamily of structurally conserved proteins. inhibitory serpins use a suicide substrate-like mechanism. some are able to inhibit cysteine proteases in cross-class inhibition. here, we demonstrate for the first time the strong inhibition of initiator and effector caspases 3 and 8 by two purified bovine serpina3s. serpina 3-1 (uniprotkb:q9tte1) binds tighly to human casp3 (uniprotkb:p42574) and casp8 (uniprotkb:q14790) with k(ass) of 4.2x10(5) and 1.4x10(6) m(-1)s(-1), respectivel ... | 2009 | 19665028 |
| sympathetic nerve varicosities in close apposition to basolateral membranes of collecting duct epithelial cells of rat kidney. | there are reports of sympathetic innervation of the nephron and of p2 purinergic receptors on epithelial cells. since atp is a cotransmitter with noradrenaline in sympathetic nerves, the objective of the present study was to re-investigate basolateral innervation of rat renal collecting duct epithelial cells by sympathetic nerves in the context of recent data on the effects of atp on this nephron segment. | 2009 | 19684415 |
| frequency measurement of the 260-thz (1.15-microm) he-ne laser. | absolute infrared frequency measurement has been extended to 260 thz with the measurement of the strong 1.15-microm laser line in (20)ne. the frequency was synthesized in nonlinear crystals of cdgeas(2) and ag(3)ass(3) from stabilized co(2) lasers and the 1.5-microm laser line in (20)ne. the measured frequency is nu(20)ne, 1.15 microm = 260.103 284(30) thz. | 1979 | 19687823 |
| identifying human and livestock sources of fecal contamination in kenya with host-specific bacteroidales assays. | microbial source tracking to distinguish between human, livestock and wildlife fecal pollution using molecular techniques is a rapidly evolving approach in many developed countries, but has not previously been applied on the african continent. dna extracts from cow, donkey, and human fecal specimens and raw domestic sewage samples collected in kenya were tested against five existing quantitative pcr assays designed to detect universal (2), human-specific (2), and cow-specific (1) fecal bacteroid ... | 2009 | 19692107 |
| unusual suicide note written on the body: two case reports. | suicide notes are commonly written on some materials, such as notebooks, papers, and mirrors. additionally, suicide plans have been communicated in face-to-face conversations, by speaking on the telephone, recording on cds or video cassettes, and sending messages through text messaging. in this article, 2 suicide death cases are presented in which suicide notes were written on the bodies of the victims. the first case, a 32-year-old man, was a university graduate and had been unemployed for year ... | 2009 | 19696586 |
| cyp46 polymorphisms in alzheimer's disease: a review. | increasing research findings argue for a link between brain cholesterol turnover and alzheimer's disease (ad). high cerebral levels of this lipid increase ass load. the elimination of cerebral cholesterol involves two mechanisms, dependent of apolipoprotein e and cholesterol 24-hydroxylase (cyp46). cyp46 is a gene associated with ad; the most studied single nucleotide polymorphism is the rs754203, which changes t-->c. some studies describe that this polymorphism is possibly associated with loss ... | 2009 | 19705089 |
| interleukin 4 receptor alpha (il4r) and calcium-activated chloride channel 1 (clca1) genes map to donkey chromosome. | the results obtained in the present study enabled the physical map of the donkey genome to be extended with markers associated with recurrent airway obstruction (rao), a major performance-limiting disease of equidae. the equine bac clone containing the il4r and clca1 genes were localized to eas 14q13 and eas 6q15 respectlivy by fluorescent in situ hybridization. identification of their locus confirmed the distribution of syntenic regions between the domestic horse and the domestic donkey within ... | 2009 | 19712222 |
| [clinical practice guidelines of the andalusian epilepsy society on prophylaxis and treatment of acute symptomatic epileptic seizures]. | antiepileptic drugs (aed) have traditionally been used empirically to prevent the presentation of epileptic seizures in patients with acute brain disorders during the early or late phase. however, aed are not free of serious drawbacks, which means that their use should be based on solid scientific foundations. our aim is to produce a set of practice guidelines based on explicit evidence about when prophylactic treatment with aed is indicated and the length of time it should be continued in acute ... | 2009 | 19714559 |
| kinetics of association and dissociation of hiv-1 reverse transcriptase subunits. | the biologically active form of hiv-1 reverse transcriptase (rt) is the p66/p51 heterodimer. the process of maturation of the heterodimer from precursor proteins is poorly understood. previous studies indicated that association of p66 and p51 is very slow. three techniques, a pre-steady-state activity assay, intrinsic tryptophan fluorescence, and a fret assay, were used to monitor the dimerization kinetics of rt. kinetic experiments were conducted with purified p66 and p51 proteins in aqueous bu ... | 2009 | 19715314 |
| prevotella dentasini sp. nov., a black-pigmented species isolated from the oral cavity of donkeys. | four strains (num 1903(t), num 1904, num 1912 and num 1925) that were obligately anaerobic, pigmented, gram-negative-staining rods were isolated from the oral cavity of donkeys. these strains were analysed using the rapid id 32a, api 20a and api zym systems, by dna-dna hybridization with other related species and by 16s rrna gene sequencing. 16s rrna gene sequence analysis showed that each of the new isolates was a member of the genus prevotella and related to prevotella multiformis pppa21(t), s ... | 2010 | 19717577 |
| sustained delivery and efficacy of polymeric nanoparticles containing osteopontin and bone sialoprotein antisenses in rats with breast cancer bone metastasis. | poor prognosis in mammary carcinoma is associated with a certain expression profile of a defined set of genes including osteopontin and bone sialoprotein. efficient and specific delivery of antisenses (as) and a protection of the sequences from degradation are the crucial conditions for as therapeutic efficiency. we hypothesized that effective and safe as delivery direceted against these genes could be achieved by polymeric nanoparticles (np) fabricated from a biocompatible polymer. due to their ... | 2010 | 19739076 |
| purification and biochemical characterization of a d-galactose binding lectin from japanese sea hare (aplysia kurodai) eggs. | a lectin was purified from japanese sea hare aplysia kurodai by lactosyl-agarose affinity chromatography. the molecular mass of the lectin was determined to be 56 and 32 kda by sds-page under non-reducing and reducing conditions, respectively. it was found to agglutinate trypsinized and glutaraldehyde-fixed rabbit and human erythrocytes in the absence of divalent cations. the lectin exhibited stable thermo-tolerance as it retained hemagglutinating activity for 1 h even at 80 degrees c and showed ... | 2009 | 19747090 |
| effects of dilution and centrifugation on the survival of spermatozoa and the structure of motile sperm cell subpopulations in refrigerated catalonian donkey semen. | the aim of this work was to study the effects of dilution and centrifugation (i.e., two methods of reducing the influence of the seminal plasma) on the survival of spermatozoa and the structure of motile sperm cell subpopulations in refrigerated catalonian donkey (equus asinus) semen. fifty ejaculates from nine catalonian jackasses were collected. gel-free semen was diluted 1:1, 1:5 or 1:10 with kenney extender. another sample of semen was diluted 1:5, centrifuged, and then resuspended with kenn ... | 2009 | 19747718 |
| ability of catalonian donkey sperm to penetrate zona pellucida-free bovine oocytes matured in vitro. | an experiment was designed to study the interaction between fresh/frozen-thawed donkey spermatozoa and zona pellucida (zp)-free bovine oocytes in an attempt to develop a model for assessing cryopreserved catalonian donkey sperm function. semen from five donkeys was collected using an artificial vagina. sperm motility and viability were immediately assessed and the semen sample cryopreserved. sperm viability and motility were then reassessed immediately after thawing. the motion characteristics o ... | 2010 | 19748750 |
| bilateral typical complete colobomas in a donkey: retinographic and fluorangiographic description. | an 18-year-old male donkey was referred with a history of blindness. on ocular examination a complete bilateral typical colobomatous defect involving iris, ciliary body, lens, choroid, retina and optic nerve was observed. an optic pit was presented at the temporal side of the right optic head. ophthalmoscopic and fluorangiographic characteristics are described. | 2009 | 19751496 |
| autoantibodies against phosphatidylserine, prothrombin and phosphatidylserine-prothrombin complex: identical or distinct diagnostic tools for antiphospholipid syndrome? | to clarify the association and diagnostic utility of measuring antiphosphatidyl serine (aps), antiprothrombin (apt) and the antiphosphatidyl serine/prothrombin complex (aps/pt) antibodies in patients with antiphospholipid syndrome (aps) and recurrent pregnancy loss (rpl). | 2009 | 19751711 |
| case control study to investigate risk factors for impaction colic in donkeys in the uk. | impaction colic is the single most common type of colic diagnosed in a large population of donkeys (more than 2000 animals) at the donkey sanctuary, uk and the fatality rate from the disease is high. here we identify risk factors for impaction colic in this population during 2006 using an unmatched case control study. there were 71 cases of impaction colic and multivariable analysis identified a number of variables associated with the disease. management factors that increased the risk of impact ... | 2009 | 19758718 |
| treatment of haemophilia in the elderly. | treatment of elderly patients with haemophilia is an upcoming challenge in haemophilia care. we included patients with haemophilia a older than 60 years of age, who visited our haemophilia centre between 2006 and 2008. we conducted a retrospective study focussing on the patients' co-morbidities as well as changes in their bleeding patterns between 2003 and 2008. results: there is a tendency of increasing bleeding symptoms with increasing age of the patients due to more frequent spontaneous joint ... | 2009 | 19763355 |
| peruvian horse sickness virus and yunnan orbivirus, isolated from vertebrates and mosquitoes in peru and australia. | during 1997, two new viruses were isolated from outbreaks of disease that occurred in horses, donkeys, cattle and sheep in peru. genome characterization showed that the virus isolated from horses (with neurological disorders, 78% fatality) belongs to a new species the peruvian horse sickness virus (phsv), within the genus orbivirus, family reoviridae. this represents the first isolation of phsv, which was subsequently also isolated during 1999, from diseased horses in the northern territory of a ... | 2009 | 19766284 |
| [construction and in vitro evaluation of an infectious clone of the equine infectious anemia virus vaccine strain eiav(fddv) with four reverse-mutated vaccine-specific sites in the s2 gene]. | to elucidate the function of the s2 gene in equine infectious anemia virus (eiav) and its role in the attenuation of the chinese attenuated eiav vaccine strains, the s2 in the eiav vaccine strain eiav (fddv) was reverse-mutated and the in vitro replication character of the resultant virus was evaluated. based on the sequence variation of the s2 gene between the eiav virulent strains and vaccine strains, all the four vaccine-specific sites in the s2 of an eiav(fddv) infectious clone, pfddv3-8, we ... | 2009 | 19769166 |
| ticks on domestic animals in pernambuco, northeastern brazil. | the objective of this article was to discuss some aspects of ticks associated with domestic animals in the state of pernambuco, northeastern brazil, based on a literature review and present new data obtained from recent tick collections carried out in this northeastern brazilian state. from august 2007 to june 2008, 1,405 ticks were collected and five species were identified: amblyomma cajennense (fabricius, 1787), amblyomma ovale koch, 1844, dermacentor nitens neumann, 1897, rhipicephalus (boop ... | 2009 | 19772771 |
| serum osteocalcin in donkeys as evaluated with an equine-specific radioimmunoassay. | the purpose of this study was to validate an equine-specific osteocalcin (oc) radioimmunoassay (ria) for use in donkeys and to establish age-related changes in serum oc concentrations in healthy donkeys. serial dilutions of donkey serum showed parallelism with standard curves obtained with the equine-specific oc ria. there was a tight linear regression between donkey serum oc values obtained with the equine specific oc ria and a commercially available bovine-specific oc ria. serum oc levels of 2 ... | 2004 | 19774757 |
| sexual behavior and ejaculate characteristics in pêga donkeys (equus asinus) mounting estrous horse mares (equus caballus). | the objectives were to (i) characterize sexual behavior of donkey stallions (jacks; equus asinus) during on-farm semen collection using estrous horse mares (mares; equus caballus); (ii) compare behavior of young (less experienced) versus older (more experienced) jacks; (iii) determine whether semen suitable for artificial insemination (ai) could be collected using mares; and (iv) determine the suitability of using mares in field collection of semen from jacks. six pêga jacks (3.5 to 16 yr old), ... | 2010 | 19775738 |
| selected health and management issues facing working donkeys presented for veterinary treatment in rural mexico: some possible risk factors and potential intervention strategies. | the examination of 216 donkeys presented for treatment at the donkey sanctuary-world horse welfare-universidad nacional autonoma de mexico mobile clinics revealed a number of health and welfare problems. a general overview of the donkeys' health was made and showed that the median body condition score (bcs) in this population was 2.5. underweight animals only accounted for 26% of the population. females, 0-5-year-olds and >21-year-olds, were more likely to be underweight. when analysed, there wa ... | 2010 | 19784862 |
| temperature and pressure dependence of ion-molecule association and dissociation reactions: the n2(+) + n2 (+ m) <--> n4+ (+ m) reaction. | thermal dissociation and the reverse association reactions of molecular ions can be represented within the same framework as unimolecular reactions of neutral molecules. however, because of different intra- and intermolecular interaction potentials, some differences between ionic and neutral systems should also be expected. starting from these generalities, the present article analyzes the temperature and pressure dependences of dissociation/association rates of ion-molecule reaction systems. th ... | 2005 | 19787982 |
| extraction of trace amount of severely degraded dna. | dna extraction from food is always problematic especially from highly processed samples which contain only trace amounts of severely degraded dna fragments. in this work, to extract trace amounts of small dna fragments of the traditional chinese medicine (tcm) colla corii asini derived from highly processed equus asinus skin, three strategies were compared for its authentication. with some optimizations, the modified qiaquick spin column method achieved higher dna yield and purity in comparison ... | 2009 | 19791512 |
| zonadhesin d3-polypeptides vary among species but are similar in equus species capable of interbreeding. | zonadhesin is a rapidly evolving protein in the sperm acrosome that confers species specificity to sperm-zona pellucida adhesion. though structural variation in zonadhesin likely contributes to its species-specific function, the protein has not previously been characterized in organisms capable of interbreeding. here we compared properties of zonadhesin in several animals, including the horse (equus caballus), donkey (e. asinus), and grevy's zebra (e. grevyi) to determine if variation in zonadhe ... | 2010 | 19794156 |
| a study of the interstitial cells of cajal in aged donkeys with and without intestinal disease. | intestinal tissue samples were collected during routine post-mortem examinations from 12 aged donkeys. six animals were euthanised due to impaction of the pelvic flexure of the large colon, while the remaining six were euthanised for non-enteric reasons such as dental or orthopaedic disease. immunohistochemical labelling was performed to demonstrate the gastrointestinal pacemaker cells, the interstitial cells of cajal (icc), with polyclonal c-kit antibodies. the distribution and density of the c ... | 2010 | 19818447 |
| alterations in lung arginine metabolism in lambs with pulmonary hypertension associated with increased pulmonary blood flow. | previous studies demonstrate impaired nitric oxide (no) signaling in children and animal models with congenital heart defects and increased pulmonary blood flow. however, the molecular mechanisms underlying these alterations remain incompletely understood. the purpose of this study was to determine if early changes in arginine metabolic pathways could play a role in the reduced no signaling demonstrated in our lamb model of congenital heart disease with increased pulmonary blood flow (shunt lamb ... | 2009 | 19818875 |
| intestinal ciliate composition found in the feces of the cypriot wild donkey, equus asinus linnaeus, 1758. | species composition and distribution of large intestinal ciliates was investigated in the feces from 13 cypriot wild donkeys, free-living in the karpaz national park, northern cyprus. we identified 16 ciliate genera and 22 species. this is the first report on intestinal ciliates in cypriot wild donkeys, and no endemic species were observed. the genus cycloposthium occurred in all animals. the mean number of ciliates was 3.0+/-2.5 x 10(4) cells ml(-1) of feces and the mean number of ciliate speci ... | 2010 | 19836214 |
| structural characterization of beta-amyloid oligomer-aggregates by ion mobility mass spectrometry and electron spin resonance spectroscopy. | formation and accumulation of fibrillar plaques and aggregates of beta-amyloid peptide (abeta) in brain have been recognized as characteristics of alzheimer's disease (ad). oligomeric aggregates of ass are considered critical intermediates leading to progressive neurodegeneration; however, molecular details of the oligomerization and aggregation pathway and the structures of abeta-oligomers are hitherto unclear. using an in vitro fibril formation procedure of abeta(1-40), beta-amyloid aggregates ... | 2009 | 19838688 |
| development of antisynthetase syndrome in a patient with rheumatoid arthritis. | rheumatoid arthritis (ra) and antisynthetase syndrome (ass) are distinct clinical syndromes, and their co-occurrence is rarely encountered. the authors report the case of a 56-year-old female patient with ra of 3 years duration who suddenly developed ass, and include a review of the literature. the patient was diagnosed with ass based on; positivity for anti-histidyl-trna synthetase (jo-1) antibody, interstitial lung disease, polyarthritis, and mechanic's hands. high-dose corticosteroid and puls ... | 2011 | 19847427 |
| effect of clopidogrel on perioperative blood loss and transfusion in coronary artery bypass graft surgery. | the effect of antiplatelet therapy (apt) on postoperative bleeding, transfusion needs and re-exploration remains unclear. this study examines the influence of apt, as well as antiplatelet mono- and combined therapy, on haemorrhage and transfusion requirements in patients undergoing coronary artery bypass on cardiopulmonary bypass (cpb). six hundred and fifty patients were reviewed retrospectively, 325 patients received apt within seven days and 325 control patients. apt group had two subgroups: ... | 2010 | 19850596 |
| violence and depressive symptoms during pregnancy: a primary care study in brazil. | to estimate the prevalence of violence, depressive symptoms, and associated factors during pregnancy in women attending antenatal care in brazil. | 2010 | 19856140 |
| sequence determination of alphas1-casein isoforms from donkey by mass spectrometric methods. | four co-eluting components, with experimentally measured m(r) of 23 658, 23 786, 24 278 and 24 406 da, were detected by reversed-phase high-performance liquid chromatography (rp-hplc) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) analysis in the dephosphorylated casein fraction of a milk sample collected at middle lactation stage from an individual donkey belonging to the ragusano breed. by coupling rp-hplc, two-dimensional polyacrylamide gel ele ... | 2009 | 19862798 |
| trypanosoma evansi infection in mainland spain. | an outbreak of trypanosoma evansi infection that occurred in mainland spain is described. the outbreak occurred on an equine and camel farm to which dromedary camels from an infected area of the canary islands had recently been introduced. one of these camels developed clinical signs and t. evansi was discovered in a blood smear examination. the herd was evaluated in order to determine the extent of the disease. the results showed that 76% of the camels, 35% of the donkeys and 2% of the horses w ... | 2010 | 19864069 |
| the 450-band resolution g- and r-banded standard karyotype of the donkey (equus asinus, 2n = 62). | donkey chromosomes were earlier characterized separately by c-, g- and r-banding techniques. however, direct comparisons between g- and r-banding patterns have still not been carried out in this species. the present study reports this comparison at the 450-band level by using replication g- and r-banding patterns. two sets of synchronized lymphocyte cultures were set up to obtain early (gba+cba-banding) and late (rba-banding) brdu incorporation. slides were stained with acridine orange and obser ... | 2009 | 19864889 |
| further study on the cultural conditions of leptospira (spirochaeta) icterohaemorrhagiae. | 1. the presence of suitable animal or human serum is essential for the cultivation of leptospira icterohaemorrhagiae. 2. the nutrient value of serum is considerably reduced by heating to 60 degrees c. for 30 minutes and is destroyed by boiling (100 degrees c). filtration through a berkefeld filter does not diminish the nutrient value of the serum. 3. the cultural value of different animal sera varies considerably. it is entirely absent from the sera of the rat and the pig. the sera of the rabbit ... | 1918 | 19868228 |
| on the antigens of red blood corpuscles : the question of lipoid antigens. | erythrocytes contain more than one substance responsible for the production of lysins and agglutinins. by injections of alcoholic extracts of horse erythrocytes mixed with a foreign serum, hemolysins and agglutinins can be more readily obtained than by the same quantities of extract alone. the immune sera reacted on horse and donkey blood but not on the blood of other species. the antibodies obtained differ from those prepared in the usual way with unchanged red blood corpuscles. the differences ... | 1925 | 19868998 |
| etiology of oroya fever : xvi. verruga in the dog and the donkey. | in the experiments here reported, definite verruga lesions, in which the presence of bartonella bacilliformis was established by culture or by passage to rhesus monkeys, were produced in a dog and in a donkey by inoculation of cultures or monkey passage strains. the reaction induced in these animals was entirely local, however; blood cultures were sterile. histologically, the lesions produced were similar to those obtained in monkeys by inoculation of bartonella bacilliformis, except for the pre ... | 1929 | 19869639 |
| differentiation of oxyhemoglobins by means of mutual solubility tests. | 1. the rule of addition of solubilities is applicable to the differentiation of the oxyhemoglobins of not too closely related species. 2. the oxyhemoglobins of the horse, dog, rat, and guinea pig show differences when tested by this method. the oxyhemoglobins of the donkey and horse show a similarity which is best explained by the assumption of isomorphism. | 1923 | 19872057 |
| cerebellar purkinje cells incorporate immunoglobulins and immunotoxins in vitro: implications for human neurological disease and immunotherapeutics. | immunoglobulin g (igg) antibodies reactive with intracellular neuronal proteins have been described in paraneoplastic and other autoimmune disorders. because neurons have been thought impermeable to immunoglobulins, however, such antibodies have been considered unable to enter neurons and bind to their specific antigens during life. cerebellar purkinje cells - an important target in paraneoplastic and other autoimmune diseases - have been shown in experimental animals to incorporate a number of ... | 2009 | 19874605 |
| pore water sampling in acid sulfate soils: a new peeper method. | this study describes the design, deployment, and application of a modified equilibration dialysis device (peeper) optimized for sampling pore waters in acid sulfate soils (ass). the modified design overcomes the limitations of traditional-style peepers, when sampling firm ass materials over relatively large depth intervals. the new peeper device uses removable, individual cells of 25 ml volume housed in a 1.5 m long rigid, high-density polyethylene rod. the rigid housing structure allows the dev ... | 2009 | 19875804 |
| detection of taylorella asinigenitalis in donkey jacks in italy. | | 2009 | 19880865 |
| the potential for estrogens in preventing alzheimer's disease. | estrogens are the best studied class of drugs for potential use in the prevention of alzheimer's disease (ad). these steroids have been shown to be potent neuroprotectants both in vitro and in vivo, and to exert effects that are consistent with their potential use in prevention of ad. these include the prevention of the processing of amyloid precursor protein (app) into beta-amyloid (abeta), the reduction in tau hyperphosphorylation, and the elimination of catastrophic attempts at neuronal mitos ... | 2009 | 19890493 |
| spontaneous disappearance of lumbar disk herniation within 3 months. | although spontaneous regression of disk herniation is a well-known phenomenon, the time taken for the condition to resolve has not been detailed in previous studies. this article describes a case of vanishing lumbar disk herniation in a 33-year-old man. the patient experienced sudden severe lumbar pain while lifting a 240-kg weight while attempting a donkey calf raise during muscle training. the pain persisted despite the use of a lumbar corset and nonsteroidal anti-inflammatory drugs. twelve da ... | 2009 | 19902881 |
| a multisystem approach for development and evaluation of inactivated vaccines for venezuelan equine encephalitis virus (veev). | a multisystem approach was used to assess the efficiency of several methods for inactivation of venezuelan equine encephalitis virus (veev) vaccine candidates. a combination of diverse assays (plaque, in vitro cytopathology and mouse neurovirulence) was used to verify virus inactivation, along with the use of a specific elisa to measure retention of veev envelope glycoprotein epitopes in the development of several inactivated veev candidate vaccines derived from an attenuated strain of veev (v35 ... | 2010 | 19903494 |
| in vivo renal arginine release is impaired throughout development of chronic kidney disease. | the kidney is a major site of arginine synthesis where citrulline is converted to arginine via argininosuccinate synthase (ass) and lyase (asl). the rate-limiting step in arginine synthesis by the normal kidney is the rate of citrulline delivery and uptake to the renal cortex. we tested whether with chronic kidney disease (ckd) renal arginine synthesis may be compromised. using the 5/6 renal ablation/infarction (a/i) injury model, we measured renal citrulline delivery and uptake as well as argin ... | 2010 | 19906948 |
| synthesis and kinetic studies of a low-molecular weight organocatalyst for phosphate hydrolysis in water. | kinetic studies of a low-molecular weight organocatalyst 1 are presented. compound 1 contains two histidines and one cationic side chain attached to a central aromatic core. in aqueous solution 1 accelerates the hydrolysis of a prototypal phosphodiester with rate enhancements of up to two orders of magnitude. a detailed hplc analysis of hydrolysis experiments in bis-tris-buffer showed that the buffer itself can act as a nucleophile at least with the cyclic phosphate 16. compound 1 is also an eff ... | 2009 | 19907780 |