| a survey of gastrointestinal pig parasites on free-range, organic and conventional pig farms in the netherlands. | the prevalence of gastrointestinal parasites in suckling piglets, weaners, fattening pigs and sows was studied from november 2001 to october 2002 on 16 free-range farms (frf), 11 organic farms (of) and 9 conventional farms (cf) by means of faecal examinations of composite samples. each farm was visited four times with a 3-month interval. infections with coccidia were found on 43.8% of the frf, 90.9% of the of and 66.7% of the cf. sows had the highest prevalence, particularly on frf (87.5%) and o ... | 2005 | 16195935 |
| the development of a mouse model to explore resistance and susceptibility to early ascaris suum infection. | ascaris suum and ascaris lumbricoides exhibit an over-dispersed frequency distribution in their host populations in both the adult and larval stages. the impact of host factors on this observed distribution is still poorly understood and difficult to investigate in the natural host populations. the use of a mouse model has been supported by the observations that the larval migratory pattern, in this host, mimics the pattern observed in the pig. we explored the extrinsic factors that might affect ... | 2006 | 16209722 |
| effect of electric power network frequency magnetic field on embryonic development of ascaris suum (nematoda). | fertilised ascaris suum eggs were subjected to an alternating electromagnetic field of frequency 50 hz and density 2 mt for 60 days. the developing embryos in both control and experimental cultures were examined daily under a microscope. the experiment resulted in an accelerated rate of embryogenesis in the eggs incubated in the electromagnetic field, higher rates of malformed embryos as well as much higher mortality rate of l2 larvae. | 2005 | 16212117 |
| nonatopic allergen-independent mast cell activation in parasitized eosinophilic athymic rats. | it is possible to induce eosinophilia in congenitally athymic rats by infection with the parasite ascaris suum. athymic bronchial eosinophilia is associated with increased expression of il-5 and eotaxin mrna, and with the presence of residual t cells and mast cells. anamnestic mastocytosis is particularly pronounced and in this study we examine the relationship between mast cell degranulation and ige production in athymic rats following infection. incubation of peritoneal mast cells from athymic ... | 2005 | 16255741 |
| unique structure of ascaris suum b5-type cytochrome: an additional alpha-helix and positively charged residues on the surface domain interact with redox partners. | cytochrome b5 of the body wall of adult ascaris suum, a porcine parasitic nematode, is a soluble protein that lacks a c-terminal membrane-anchoring domain, but possesses an n-terminal pre-sequence of 30 amino acids. during the maturation of cytochrome b5, the n-terminal pre-sequence is proteolytically cleaved to form the mature protein of 82 amino acid residues. a. suum cytochrome b5 is a basic protein containing more lysine residues and exhibiting a higher midpoint redox potential than its mamm ... | 2006 | 16288599 |
| inactivation of ascaris suum eggs by ammonia. | uncharged ammonia is known to cause inactivation of a number of wastewater pathogens, but its effect on ascaris eggs has never been isolated or quantified. the objectives of this research were to determine the conditions under which ammonia inactivates eggs of the swine ascaris species, ascaris suum, and to quantify the impact of ammonia on the u.s. epa's requirements for alkaline treatment to produce class a sludge. eggs were incubated in controlled, laboratory solutions such that the effects o ... | 2005 | 16295855 |
| preparation and characterization of specific monoclonal antibodies for the detection of adult worm infections in onchocerciasis. | suitable molecular tests for monitoring the viability of adult worms of onchocerca in vivo are required to accelerate the development of new macrofilaricides in river blindness (onchocerciasis). hence, three monoclonal antibodies (mabs) were prepared and evaluated in a sandwich enzyme-linked immunosorbent assay (elisa) for their abilities to detect circulating adult worm antigens in onchocercal bovine and human sera. the mabs did not cross-react with a number of control antigens, which included ... | 2005 | 16332194 |
| non-invasive assessment of airway responsiveness in healthy and allergen-sensitised cats by use of barometric whole body plethysmography. | this study aimed at determining whether airway responsiveness (ar) tests performed by use of barometric whole body plethysmography (bwbp) were repeatable in cats and to what extent ar was affected by the nebulization protocol used, the age of the animals, the inflammatory status of the airways and prior bronchodilator treatment. repeatability of ar was tested on two occasions in 30 healthy cats. the concentration of carbachol inducing a 300% increase of the enhanced pause (penh)--an estimator of ... | 2007 | 16359894 |
| laboratory evaluation of thermophilic-anaerobic digestion to produce class a biosolids. 2. inactivation of pathogens and indicator organisms in a continuous-flow reactor followed by batch treatment. | thermophilic-anaerobic digestion in a single-stage, mixed, continuous-flow reactor is not approved in the united states as a process capable of producing class a biosolids for land application. this study was designed to evaluate the inactivation of pathogens and indicator organisms in such a reactor followed by batch treatment in a smaller reactor. the combined process was evaluated at 53 degrees c with sludges from three different sources and at 51 and 55 degrees c with sludge from one of the ... | 2005 | 16381150 |
| cross-species rnai: selected ascaris suum dsrnas can sterilize caenorhabditis elegans. | | 2006 | 16388861 |
| drug transport mechanisms in helminth parasites: passive diffusion of benzimidazole anthelmintics. | anthelmintic molecules must reach their receptors inside target parasites to exert the pharmacological effect. available data suggest that the main route of entry of antiparasitic drugs into helminth parasites would be through their external surface. however, it is unclear if trans-tegumental/cuticular penetration is the most important way of entry of benzimidazole (bzd) anthelmintics into their target parasites compared to oral ingestion. the relative involvement of active and passive transport ... | 2006 | 16430886 |
| dissecting ascaris glycosphingolipids for immunomodulatory moieties--the use of synthetic structural glycosphingolipid analogues. | we have previously shown glycosphingolipids of ascaris suum to have phosphorylcholine (pc) and non-pc immunomodulatory moieties. in the present study we further investigated the nature of the immunomodulatory moieties by employing three synthetic glycosphingolipids each possessing features of the original molecule to examine effects on macrophage and dendritic cell (dc) cytokine production and surface co-stimulatory molecule expression. compound 2, which lacked pc but contained ceramide, had no ... | 2006 | 16441504 |
| the complete mitochondrial genome of anisakis simplex (ascaridida: nematoda) and phylogenetic implications. | we determined the nucleotide sequence of the complete mitochondrial genome of the nematode species anisakis simplex. the genome is circular, 13,916 bp in size and conforms to the general characteristics of nematode mitochondrial dnas. the gene arrangement of a. simplex is the same as that of ascaris suum and almost identical to those of rhabditid species with a minor exception concerning the relative position of the at-rich and non-coding regions and radically different from those of spirurid sp ... | 2006 | 16442542 |
| deep-breath frequency in bronchoconstricted monkeys (macaca fascicularis). | deep-breath frequency has been shown to increase in spontaneously obstructed asthmatic subjects. furthermore, deep breaths are known to be regulated by lung rapidly adapting receptors, yet the mechanism by which these receptors are stimulated is unclear. this study tested the hypothesis that deep-breath frequency increases during experimentally induced bronchoconstriction, and the magnitude of the increased deep-breath frequency is dependent on the method by which bronchoconstriction is induced. ... | 2006 | 16467390 |
| fertilization state of ascaris suum determined by electrorotation. | electrorotation is a non-invasive technique that is capable of detecting changes in the morphology and physicochemical properties of microorganisms. the first detailed electrorotation study of the egg (ovum) of a parasitic nematode, namely ascaris suum is described to show that electrorotation can rapidly differentiate between fertilized and non-fertilized eggs. support for this conclusion is by optical microscopy of egg morphology, and also from modelling of the electrorotational response. mode ... | 2006 | 16469169 |
| influence of season and host age on wild boar parasites in corsica using indicator species analysis. | the indicator value (ind val) method which combines measures of fidelity and specificity has been used in a study on wild boar parasites in corsica during 2001-2003. because of its resilience to changes in abundance, indval is a particularly effective tool for ecological bioindication. the ind val method showed how season can influence the occurrence of parasite species in the wild boar, and also identified parasites as bioindicators relative to host age. the randomization test identified five p ... | 2006 | 16469171 |
| optimum activity of the phosphofructokinase from ascaris suum requires more than one metal ion. | phosphofructokinase (pfk) catalyzes the phosphorylation of fructose 6-phosphate (f6p) to give fructose 1,6-bisphosphate (fbp) using mgatp as the phosphoryl donor. as the concentration of mg(2+) increases above the concentration needed to generate the mgatp chelate complex, a 15-fold increase in the initial rate was observed at low mgatp. the effect of mg(2+) is limited to v/k(mgatp), and initial rate studies indicate an equilibrium-ordered addition of mg(2+) before mgatp. isotope partitioning of ... | 2006 | 16475835 |
| measurement of one-bond 13calpha-1halpha residual dipolar coupling constants in proteins by selective manipulation of calphahalpha spins. | we have developed new 2d and 3d experiments for the measurement of c(alpha)-h(alpha) residual dipolar coupling constants in (13)c and (15)n labelled proteins. two experiments, 2d (hnco)-(j-ca)nh and 3d (hn)co-(j-ca)nh, sample the c(alpha)-h(alpha) splitting by means of c(alpha) magnetization, while 2d (j-hacaco)nh and 3d j-ha(caco)nh use h(alpha) magnetization to achieve a similar result. in the 2d experiments the coupling evolution is superimposed on the evolution of the (15)n chemical shifts a ... | 2006 | 16495100 |
| a case of ascaris suum visceral larva migrans diagnosed by using a. suum larval excretory-secretory (es) antigen. | a 42-y-old female presented with common cold-like symptoms. laboratory data showed mild liver dysfunction together with peripheral blood eosinophilia. she was suspected of having a helminthic infection, however parasite eggs or larvae were not detected by repeated stool examinations. eventually she transpired to have a high igg antibody titer against excretory-secretary (es) antigen of ascaris suum larvae, but not of toxocara canis larvae, suggesting that she had been suffering from visceral lar ... | 2006 | 16500788 |
| inactivation of single-celled ascaris suum eggs by low-pressure uv radiation. | intact and decorticated single-celled ascaris suum eggs were exposed to uv radiation from low-pressure, germicidal lamps at fluences (doses) ranging from 0 to 8,000 j/m2 for intact eggs and from 0 to 500 j/m2 for decorticated eggs. with a uv fluence of 500 j/m2, 0.44-+/-0.20-log inactivation (mean+/-95% confidence interval) (63.7%) of intact eggs was observed, while a fluence of 4,000 j/m2 resulted in 2.23-+/-0.49-log inactivation (99.4%). (the maximum quantifiable inactivation was 2.5 log units ... | 2006 | 16517669 |
| pas-1, a protein affinity purified from ascaris suum worms, maintains the ability to modulate the immune response to a bystander antigen. | helminth infections and parasite components have potent immunomodulatory effects on a host's immune system. in the present study, we investigated the effect of pas-1, a protein component of ascaris suum adult worms recognized by a monoclonal antibody (maip-1), on humoral and cell-mediated responses to a bystander antigen (ovalbumin [ova]). maip-1 recognized only one of the three polypeptide chains of pas-1, but neutralized the suppressive effect of the whole worm extract on ova-specific antibody ... | 2006 | 16519731 |
| molecular evidence for the infection of zoo chimpanzees by pig ascaris. | we here describe the transmission of the pig roundworm, ascaris suum to chimpanzees maintained in the copenhagen zoo, denmark. using a technique for whole genome fingerprinting, amplified fragment length polymorphism (aflp) and the technique pcr restricted fragment length polymorphism (pcr-rflp) of the internal transcribed spacer region (its) of the nuclear ribosomal dna, the worms from the chimpanzees were compared with ascaris spp obtained from humans and pigs in order to identify the source o ... | 2006 | 16567044 |
| the ever-expanding neuropeptide gene families in the nematode caenorhabditis elegans. | neuropeptides act as chemical signals in the nervous system to modulate behaviour. with the ongoing est projects and dna sequence determination of different genomes, the identification of neuropeptide genes has been made easier. despite the relatively 'simple' repertoire of behaviours in the nematode caenorhabditis elegans, this worm contains a surprisingly large and diverse set of neuropeptide genes. at least 109 genes encoding over 250 potential neuropeptides have been identified in c. elegans ... | 2005 | 16569285 |
| gene expression and pharmacology of nematode nlp-12 neuropeptides. | this study examines the biology of nlp-12 neuropeptides in caenorhabditis elegans, and in the parasitic nematodes ascaris suum and trichostrongylus colubriformis. dyrplqfamide (1 nm-10 microm; n > or =6) produced contraction of innervated dorsal and ventral ascaris body wall muscle preparations (10 microm, 6.8+/-1.9 g; 1 microm, 4.6+/-1.8 g; 0.1 microm, 4.1+/-2.0 g; 10 nm, 3.8+/-2.0 g; n > or =6), and also caused a qualitatively similar, but quantitatively lower contractile response (10 microm, ... | 2006 | 16600246 |
| dermal microdialysis in the dog: in vivo assessment of the effect of cyclosporin a on cutaneous histamine and prostaglandin d2 release. | dermal microdialysis, a relatively noninvasive technique, allows investigation of the changes in cellular mediators released during cutaneous allergic responses. this technique was used to evaluate the effect of cyclosporin a, an immunosuppressive drug used for treatment of canine atopic dermatitis, on the cutaneous release of two pro-inflammatory mediators following intradermal allergen challenge. four beagle dogs spontaneously sensitized to ascaris suum were treated for 1 month with oral cyclo ... | 2006 | 16674731 |
| pulmonary lesions associated with visceral larva migrans due to ascaris suum or toxocara canis: imaging of six cases. | objective: the objective of our study was to evaluate chest radiographic and ct findings of patients with pulmonary lesions associated with visceral larva migrans due to ascaris suum or toxocara canis. ct investigation was focused on the location, size, contour, and internal features of the lesions; migration of lesions; mediastinal lymphadenopathy; and pleural effusion. conclusion: pulmonary visceral larva migrans appears on ct as multifocal subpleural nodules with halo or ground-glass opacitie ... | 2006 | 16714661 |
| prevalence of common gastrointestinal nematode parasites in scavenging pigs of different ages and sexes in eastern centre province, burkina faso. | the range and infestation intensities of gastrointestinal parasitic nematode species depend on the type of swine production system. the present study focused mainly on nematodes of veterinary importance in scavenging pigs in burkina faso, and aimed at determining the prevalence of gastro-intestinal nematode parasites by means of faecal egg per gram (epg) counts. between november 2001 and october 2002, faecal samples from 383 pigs of different sexes and ages (< 5 months, 5-12 months and > 12 mont ... | 2006 | 16715878 |
| isolation and identification of three new 5-alkenyl-3,3(2h)-furanones from two streptomyces species using a genomic screening approach. | analyses of biosynthetic gene clusters derived from streptomyces aculeolatus nrrl 18422 and streptomyces sp. eco86 indicated that both microorganisms have similar type i polyketide synthase (pks) gene clusters with relatively few genes encoding post-pks elaborative enzymes. however both gene clusters included a sequence coding for a relatively uncommon oxidative enzyme related to baeyer-villiger, flavin-type monooxygenases. screening of culture extracts for compounds with the predicted physicoch ... | 2006 | 16724457 |
| ascaris suum infections in pigs born and raised on contaminated paddocks. | the transmission of ascaris suum was studied in outdoor reared pigs. from may to june 2001, 6 farrowing paddocks were naturally contaminated with a. suum using experimentally infected seeder pigs. early july, 1 sow farrowed on each paddock. one piglet per litter was slaughtered every second week starting at week 3 post-partum (p.p.) for registration of liver white spots and recovery of a. suum from the lungs and the small intestine. the last pigs were slaughtered at week 19 p.p. faeces was exami ... | 2006 | 16740179 |
| regulation of anaphylactic igg1 antibody production by il-4 and il-10. | different cytokines have been implicated in the regulation of isotype expression in primary and secondary antibody responses. the aim of this study was to assess the regulation of anaphylactic igg1 and ige antibodies by il-4, il-10 and ifn-gamma at different time points of the antibody response against pi, an immunosuppressive fraction of ascaris suum extract, and ovalbumin (ova). | 2006 | 16804329 |
| mri studies of spinal visceral larva migrans syndrome. | we report serial mr findings in four patients with myelitis caused by visceral larva migrans syndrome due to toxocara canis or ascaris suum infection. mr imaging revealed spinal cord swelling with or without gadolinium enhancement in three patients. t2-weighted images showed high signal intensities preferentially located in both lateral and posterior columns. antihelmintic and corticosteroid treatment yielded improvement in neurologic deficits and spinal lesions. however, one patient with t. can ... | 2006 | 16815465 |
| whole-body periodic acceleration modifies experimental asthma in sheep. | nitric oxide is released from vascular endothelium in response to increased pulsatile shear stress. nitric oxide inhibits mast cell activation and is antiinflammatory and therefore might be protective in asthma. | 2006 | 16858016 |
| activity of hydrolases in the reproductive system and in consecutive stages of ascaris suum development. | the activity of hydrolases in the eggs isolated from the uterus of a. suum is in most cases similar to that in the reproductive system. the activity of hydrolases was low in the stages of cleavage and gastrulation, and it grew until the larva stage. | 2004 | 16859035 |
| a protein extension to shorten rna: elongated elongation factor-tu recognizes the d-arm of t-armless trnas in nematode mitochondria. | nematode mitochondria possess extremely truncated trnas. of 22 trnas, 20 lack the entire t-arm. the t-arm is necessary for the binding of canonical trnas and ef (elongation factor)-tu (thermo-unstable). the nematode mitochondrial translation system employs two different ef-tu factors named ef-tu1 and ef-tu2. our previous study showed that nematode caenorhabditis elegans ef-tu1 binds specifically to t-armless trna. c. elegans ef-tu1 has a 57-amino acid c-terminal extension that is absent from can ... | 2006 | 16859488 |
| [comparative studies on the difference between ascaris lumbricoides and ascaris suum]. | there has been continued controversy on the taxonomy of ascaris lumbricoides linnaeus, 1758 from humans and ascaris suum goeze, 1782 from pigs. this article reviews a range of comparative studies related to host susceptibility, morphology, karyotype, immunology and biochemistry, as well as molecular genetics in recent years. | 2006 | 16862915 |
| the effect of low temperatures on the development of eggs of ascaris suum goeze, 1782. | eggs of a. suum were kept at -10 and -20 degrees c for 12 weeks and subsequently incubated in a thermostat at 28 degrees c. during the incubation, their development was checked every 5 days. it was found that storage at low temperatures slows down their development and reduces the number of eggs successfully completing their embryogenesis. | 2004 | 16865961 |
| effect of ascaris chymotrypsin inhibitor on fetal development of mice. | balb/c mice were given daily doses of 40-80 mg of ascaris chymotrypsin inhibitor /achi/ per kg body weight from 12th until 15th day of gestation (stage of fetal development). it has been found that injection of achi disturbed the course of pregnancy (bleeding from uterus, abortions, decreased body weight gain as compared to control /p < 0.05/). achi exhibited embryotoxic effects (a high rate of intrauterine deaths, decreased number of living fetuses and mean fetal weight, delayed skeletal ossifi ... | 2004 | 16865962 |
| [prevalence of eimeria and intestinal nematodes in wild boar in north-west poland]. | in the time from september 2003 to march 2004 feacal samples of 147 wild boars from north-west poland were examined. the prevalence and intensity of the coccidia and gastro-intestinal nematodes infection were determined by means of willis-schlaaf. the species were determined basing on morphology of oocysts (shape, colour, form index, presence or absence of micropyle and its cap, presence or absence of residual, polar and stied bodies) and time of sporulation. sporulation was performed in wet cha ... | 2004 | 16865979 |
| antibody responses to ascaris-derived proteins and glycolipids: the role of phosphorylcholine. | in addition to proteins, glycolipids can be targets of antibody responses and contribute to host-pathogen interaction. following the structural analysis of ascaris lumbricoides-derived glycolipids, the antibody responses of a group of children with no, light and heavy infections were analysed. the role of the phosphorylcholine moiety, present on ascaris glycoproteins and glycolipids, in antibody reactivity of these infected individuals was determined. children carrying heavy infections showed hi ... | 2006 | 16879308 |
| developmental abnormalities in chicken embryos after injection proteolysis inhibitors from ascaris suum. | it has been found that trypsin and alpha-chymotrypsin inhibitors isolated from ascaris suum act embryotoxically and teratogenically on white leghorn chicken embryos. mortality rate for the chicken embryos on day 15 of incubation was 45.0 +/- 3.5% after injection of trypsin inhibitor and 44.0 +/- 3.5% after adminstration alpha-chymotrypsin inhibitor. gross examination of surviving embryos and their dissection revealed pathological changes (abdominal dropsy, umbilical hernia, subcutaneous oedema, ... | 2002 | 16883703 |
| [influence of zinc and lead ions on the development of eggs of ascaris suum (nematoda)]. | the objective of this work was to examine the influence of heavy metals ions, zinc and lead, in external environment on the development of ascaris suum to invasive stage. the culture mediums contained 25.5, and 0.5 mm pb(no3)2 or znso4. after 6 weeks of development in 26 degrees c in the control 75.17% of eggs reached the invasive stage. ions of lead and zinc, in a way depending on concentration inhibited development of embryos. the invasive stage in the presence of 25.5 and 0.5 mm pb(no3)2 were ... | 2000 | 16886332 |
| comparison of the effect of ascaris trypsin inhibitor in various periods of mouse pregnancy. | trypsin inhibitor isolated from ascaris suum and injected into pregnant balb/c mice (five times, in doses: 300 or 400 mg/kg/day) in various periods of pregnancy (early and late organogenesis) disturbed the development of fetuses. the nature and intensity of prenatal disturbances are determined by the inhibitor dose and time of injection. it has been found that administration of the inhibitor from 5-th until 9-th day of gestation did not delay or prevent implantation, but caused a high rate of in ... | 2001 | 16886413 |
| the effect of ascaris trypsin inhibitor on proteolytic activity of lungs and kidneys of guinea pig with larval ascariosis and after ascarid homogenate administration. | trypsin inhibitor, isolated from ascaris suum tegument reduces in vitro proteolytic activity (ph 7) of lungs and kidneys post-nuclear fractions of guinea pigs with larval ascariosis, pigs after subcutaneous ascarid homogenate administration as well as the activity in control groups. the comparative analysis of effect curves of trypsin inhibitor on the organ proteases revealed stronger reduction of the activity of post-nuclear lung proteases in ascariosis and of kidneys proteases after the inject ... | 2001 | 16886415 |
| the alterations in genetic apparatus of somatic and generative cells of the host caused by helminths metabolites. | alterations in the genetic apparatus of mice bone marrow cells and testicles caused by hymenolepis nana, ascaris suum and toxocara canis metabolites have been investigated by means of micronuclear test application. the activity of spermatogenesis has been investigated at experimental hymenolepidosis, migrating ascariasis and toxocarosis with the use of 3h-timidine. helminths metabolites have been established to exert a mutagenic effect on somatic cells of bone marrow, spermatogonies and also on ... | 2001 | 16886443 |
| preliminary evaluation of maternotoxic effect of ascaris extract in mice. | administration intraperitoneally of the ascaris suum extract--ase-(0.6-1.4 g of ascaris proteins/kg/day) at a late stage of organogenesis (8-12 days of gestation) disturbed course of mouse pregnancy. it has been found that injections of higher doses of ase to pregnant mice caused the symptoms manifesting maternal toxicity (decreased body weight gain/p < 0.001/as compared to control, intrauterine resorption of litter, vaginal hemorrhages, female mortality and altered behaviour). there is a linear ... | 2003 | 16889025 |
| early stages of ascaris suum induce airway inflammation and hyperreactivity in a mouse model. | the inflammatory and functional changes that occur in murine lung after infection with 2500 infective ascaris suum eggs were studied in this work. a sequential influx of neutrophils, mononuclear cells and eosinophils occurred into airways concomitantly with migration of larvae from liver to the lungs. histological analysis of the lung showed a severe intra-alveolar haemorrhage at the peak of larval migration (day 8) and the most intense inflammatory cell infiltrate on day 14. ascaris l3 were fou ... | 2006 | 16916369 |
| fluoride exposure inhibits protein expression and enzyme activity in the lung-stage larvae of ascaris suum. | sodium fluoride (naf) is an anion that has been previously shown to block the moulting process of ascaris suum larvae. this study describes moulting and development-specific protein expression profiles of a. suum lung-stage l3 (asll3) following naf exposure. asll3s cultured in the presence or absence of naf were prepared for protein analysis using two-dimensional (2d) electrophoresis. naf exposure inhibited at least 22 proteins in asll3 compared with moulted larvae (i.e. asll4). a further compar ... | 2006 | 16959050 |
| ascaris suum-derived products suppress mucosal allergic inflammation in an interleukin-10-independent manner via interference with dendritic cell function. | we have previously demonstrated that protection from allergic inflammation by ascaris suum infection was characterized by a global increase in interleukin-10 (il-10) and the development of protective cd4(+)/cd25(+) t cells (l. schopf, s. luccioli, v. bundoc, p. justice, c. c. chan, b. j. wetzel, h. h. norris, j. f. urban, jr., and a. keane-myers, investig. ophthalmol. vis. sci. 46:2772-2780, 2005). here, we used a. suum pseudocoelomic fluid (pcf) in lieu of infection to define molecular mechanis ... | 2006 | 16966410 |
| population structure in ascaris suum (nematoda) among domestic swine in denmark as measured by whole genome dna fingerprinting. | we here analyze the population structure in the pig roundworm, ascaris suum, among domestic pigs in denmark using a whole-genome dna fingerprinting technique, "amplified fragment length polymorphism" (aflp) analysis. with these data, we can extract absolute gene frequency variance components and g-statistics for 135 independent nucleotide polymorphisms. the average proportion of total variance partitioned between jutland and zealand is less than 3% of the total variance, implying no restriction ... | 2005 | 16970605 |
| effect of piperazine (diethylenediamine) on the moulting, proteome expression and pyrophosphatase activity of ascaris suum lung-stage larvae. | piperazine (diethylenediamine) is an anthelmintic widely used against animal and bird ascariasis. in this study, we show that treatment with piperazine blocks ascaris suum larval moulting and development processes and affects larval proteome expression profiles. a. suum lung-stage l3 (ll3) obtained from an infected rabbit's lungs were cultured in rpmi medium in the presence of increasing concentrations of piperazine sulfate (pzes). our results showed that pzes potently inhibited moulting of a. s ... | 2006 | 16987490 |
| efficacy of il-13 neutralization in a sheep model of experimental asthma. | il-13 contributes to airway hyperresponsiveness, mucus secretion, inflammation, and fibrosis, suggesting that it plays a central role in asthma pathogenesis. neutralization of il-13 with sil-13ralpha2-fc (sil-13r) reduces allergen-induced airway responses in rodent models of respiratory disease, but its efficacy in a large animal model has not been previously reported. in this study, we determined whether two different strategies for il-13 neutralization modified experimental asthma in sheep. sh ... | 2007 | 17023688 |
| a real-time pcr method for quantifying viable ascaris eggs using the first internally transcribed spacer region of ribosomal dna. | worldwide, 1.4 billion people are infected with the intestinal worm ascaris lumbricoides. as a result, ascaris eggs are commonly found in wastewater and sludges. the current microscopy method for detecting viable ascaris eggs is time- and labor-intensive. the goal of this study was to develop a real-time quantitative pcr (qpcr) method to determine the levels of total and viable ascaris eggs in laboratory solutions using the first internally transcribed spacer (its-1) region of ribosomal dna (rdn ... | 2006 | 17056687 |
| pharmacology of n-, l-, and b-subtypes of nematode nachr resolved at the single-channel level in ascaris suum. | pharmacological experiments on ascaris suum have demonstrated the presence of three (n-, l-, and b-) subtypes of cholinergic receptor mediating contraction of body wall muscle in parasitic nematodes. in the present study, these ionotropic acetylcholine (ach) receptors (nachrs) were activated by levamisole and bephenium under patch-clamp conditions and competitively antagonized by paraherquamide and 2-desoxoparaherquamide. a number of recordings exhibited three separate current amplitude levels, ... | 2006 | 17056760 |
| elisa antibody determination in patients with anisakiosis or toxocariosis using affinity chromatography purified antigen. | one of the fundamental aspects of a parasitic infection diagnosis is the use of adequate antigens to develop specific and sensitive immunoassays. this fact is especially complicated in nematode infection cases because of the high cross-reactivity among different parasites in this group. we performed an evaluation of anisakis simplex antigens purified by affinity chromatography. we used sera from 38 patients diagnosed with anisakis sensitization and sera from 35 patients with clinical suspicion o ... | 2006 | 17063674 |
| a combined bioinformatics and chemoinformatics approach for the development of new antiparasitic drugs. | a modern concept for the development of novel antiparasitic drugs is the combination of bioinformatics and chemoinformatics approaches. this covers, for example, the identification of target proteins serving as molecular points of attack for parasiticides--the idea is that, owing to some essential role, inhibition of a target protein should eradicate the parasite. to prevent toxicity problems for vertebrate host organisms, it is advantageous that these proteins show significant differences from ... | 2007 | 17070005 |
| l3l4es antigen and secretagogues induce histamine release from porcine peripheral blood basophils after ascaris suum infection. | the aim of this paper was to investigate the role of porcine basophils in protective immunity. experimental pigs were infected with 10(3) ascaris suum eggs daily for 21 days. control pigs were maintained helminth-free. circulating porcine basophils were isolated from the anticoagulated whole blood of a. suum-infected and noninfected pigs by dextran (4.5%) sedimentation of erythrocytes or by the centrifugation of dextran-isolated leukocytes through discontinuous percoll gradients. results showed ... | 2007 | 17096138 |
| immunosuppressive components of ascaris suum down-regulate expression of costimulatory molecules and function of antigen-presenting cells via an il-10-mediated mechanism. | high-molecular-weight components (pi) of ascaris suum suppress both cell-mediated and humoral responses against ovalbumin (ova) via an il-4/il-10-dependent mechanism. the aim of this work was to investigate the effect of pi on the ability of apc to activate t cells and the role of il-10 in this process. flow cytometry analyses of mhc class ii, cd80, cd86 and cd40 molecules on ln cells from mice immunized with ova or ova+pi showed that pi inhibits expression of these molecules on unfractionated c ... | 2006 | 17109466 |
| operon conservation and the evolution of trans-splicing in the phylum nematoda. | the nematode caenorhabditis elegans is unique among model animals in that many of its genes are cotranscribed as polycistronic pre-mrnas from operons. the mechanism by which these operonic transcripts are resolved into mature mrnas includes trans-splicing to a family of sl2-like spliced leader exons. sl2-like spliced leaders are distinct from sl1, the major spliced leader in c. elegans and other nematode species. we surveyed five additional nematode species, representing three of the five major ... | 2006 | 17121468 |
| [the influence of the metarhizium fungi on the embryonic development of ascaris suum]. | purpose: the present study focused on the effect of two fungal species metarhizium flavoviride and metarhizium anisopliae on the embryonic development and viability of ascaris suum larvae. results: microscope examination revealed that the presence of the mycelium inhibited the egg development, compared to control. the fungus-exposed eggs featured zygote vacuolisation, irregular blastomere cleavage, and morphological disturbances in the stages of blastula, gastrula, and larva. the embryopathies w ... | 2006 | 17131843 |
| paecilaminol, a new nadh-fumarate reductase inhibitor, produced by paecilomyces sp. fki-0550. | a new nadh-fumarate reductase inhibitor, paecilaminol, was isolated from the cultured broth of a fungus paecilomyces sp. fki-0550. it is an amino alcohol compound, the structure being established as 2-amino-14,16-dimethyl-3-octadecanol. paecilaminol exhibited an ic50 value of 5.1 microm against ascaris suum nadh-fumarate reductase. | 2006 | 17136891 |
| n-glycans of the porcine nematode parasite ascaris suum are modified with phosphorylcholine and core fucose residues. | in recent years, the glycoconjugates of many parasitic nematodes have attracted interest due to their immunogenic and immunomodulatory nature. previous studies with the porcine roundworm parasite ascaris suum have focused on its glycosphingolipids, which were found, in part, to be modified by phosphorylcholine. using mass spectrometry and western blotting, we have now analyzed the peptide n-glycosidase a-released n-glycans of adults of this species. the presence of hybrid bi- and triantennary n- ... | 2007 | 17181538 |
| effects of high hydrostatic pressure on embryonation of ascaris suum eggs. | high hydrostatic pressure processing (hpp) has been shown to be an effective non-thermal means of inactivating microorganisms from various food products. little information is available regarding the effects of hpp on metazoan parasites. outbreaks of food-borne disease have been associated with importation of food contaminated with fecal material. ascaris suum is used as a surrogate model metazoan parasite for the human roundworm, ascaris lumbricoides, to study the effects of treatments on the i ... | 2007 | 17187930 |
| sensitization of naive beagles by intradermal injection of an ascaris antigen: induction of a model of skin allergy. | ascaris suum-hypersensitive beagles represent a unique model of skin allergy. despite its suitability, the need to test numerous dogs prior to the selection of natural positive responders causes many complications. we hypothesized that the model could be induced in adult beagles by primary sensitization. ten dogs were included in an intradermal sensitization program using ascaris antigen and were thereafter tested for cutaneous reaction. after 2 weeks, 60% developed a positive reaction that last ... | 2006 | 17190744 |
| effects of age and allergen-induced airway inflammation in cats: radiographic and cytologic correlation. | thoracic radiography is an important diagnostic tool for feline respiratory medicine. the aim of this study was (1) to assess age-related changes of thoracic radiographic views in healthy young cats and (2) to test if experimentally-induced bronchial inflammation by inhaling ascaris suum (as) allergens leads to radiographic changes after single or repeated exposures. healthy cats (n=15-30) aged between 6 and 30 months were evaluated. eight healthy cats and eight as-sensitised cats, respectively, ... | 2007 | 17218130 |
| [the time of development of ascarid eggs in the soil of the central chernozem zone (in case of the kursk region)]. | | 2006 | 17290910 |
| functional, inflammatory and morphological characterisation of a cat model of allergic airway inflammation. | the aims of this study were to characterise a model of feline allergic airway inflammation and to test through a longitudinal investigation whether five repeated allergen exposures would lead to signs of airway remodelling that would be detectable in vivo. eight healthy control cats and eight cats sensitised with ascaris suum allergens were investigated. barometric whole body plethysmography (bwbp) was used for the assessment of respiratory variables and airway responsiveness (ar). bronchoalveol ... | 2007 | 17306576 |
| verticipyrone, a new nadh-fumarate reductase inhibitor, produced by verticillium sp. fki-1083. | a new nadh-fumarate reductase inhibitor, verticipyrone, was isolated from the cultured broth of a fungus, verticillium sp. fki-1083. the structure was established as (e)-2-methoxy-3,5-dimethyl-6-(3-methyl-2-undecenyl)-4h-pyran-4-one. verticipyrone exhibited an ic50 value of 0.88 nm against nadh-fumarate reductase of ascaris suum. verticipyrone inhibited both ascaris and bovine heart complex i, and its synthetic analogue, 8,9-dihydro-8-hydroxyverticipyrone, showed good selectivity against ascaris ... | 2006 | 17323645 |
| a ser/thr kinase required for membrane-associated assembly of the major sperm protein motility apparatus in the amoeboid sperm of ascaris. | leading edge protrusion in the amoeboid sperm of ascaris suum is driven by the localized assembly of the major sperm protein (msp) cytoskeleton in the same way that actin assembly powers protrusion in other types of crawling cell. reconstitution of this process in vitro led to the identification of two accessory proteins required for msp polymerization: an integral membrane phosphoprotein, msp polymerization-organizing protein (mpop), and a cytosolic component, msp fiber protein 2 (mfp2). here, ... | 2007 | 17344482 |
| the effect of opiates and opiate antagonists on heat latency response in the parasitic nematode ascaris suum. | the effects of the opiates morphine and morphine-6-glucuronide (m6g), the mu opioid receptor specific antagonist d-phe-cys-tyr-d-trp-om-thr-pen-thr-nh(2) (ctop), and the general opiate antagonist naloxone on the latency of response to thermal stimulation were determined in the parasitic nematode ascaris suum. thermal detection and avoidance behaviors of the worms were evaluated with a tail flick analgesia meter using a modification of a technique employed for nociception experiments in rodents. ... | 2007 | 17363006 |
| il-13 blockade reduces lung inflammation after ascaris suum challenge in cynomolgus monkeys. | airway inflammation is a hallmark feature of asthma and a driver of airway hyperresponsiveness. il-13 is a key inducer of airway inflammation in rodent models of respiratory disease, but a role for il-13 has not been demonstrated in primates. | 2007 | 17379289 |
| migration behaviour and pathogenesis of five ascarid nematode species in the mongolian gerbil meriones unguiculatus. | to understand the characteristic features of the mongolian gerbil, meriones unguiculatus, as an animal model of ascarid infections, the migration behaviour and pathogenesis of larvae were investigated in experimentally infected gerbils. embryonated eggs from each of toxocara canis, baylisascaris procyonis, b. transfuga, ascaris suum, and a. lumbricoides were orally inoculated into gerbils and larvae were recovered from various organs at designated periods. in t. canis-infected gerbils, larvae we ... | 2007 | 17381866 |
| the migration of ascaris suum larvae, and the associated pulmonary inflammatory response in susceptible c57bl/6j and resistant cba/ca mice. | ascariasis is an important infection in humans (ascaris lumbricoides) and pigs (ascaris suum) and individuals appear to be predisposed to either heavy or light worm burdens. these extremes of susceptibility and resistance are represented in a mouse model by 2 strains of mice, cba mice showing high resistance to infection and c57bl/6 which are highly susceptible, as reflected in worm burdens in the lungs 6-7 days after infection. in an attempt to identify the point at which the difference between ... | 2007 | 17381887 |
| [limitation in the population of parasitic geohelminths by saprotrophic soil fungi and their secretions]. | the aim of the study was to examine the effect of mould fungi isolated from soil and of two of their metabolites, aflatoxin g1 and ochratoxin a, on the embryonic development of eggs and mortality of larvae of ascaris suum. an attempt was made to demonstrate synergism between fungi species in the action on embryogenesis of ascaris suum. | 2006 | 17385347 |
| partial characterization of the antigen recognized by a monoclonal antibody to ascaris suum ovary extracts. | a monoclonal antibody produced against ovary extracts from the worm ascaris suum showed immunoreactivity against granules in the rachis and oocytes, the inner layer of the eggshell and the middle layer of some egg, but not against either ovary wall or uterus wall. furthermore, the same antigens were detected on the body surface of migrated larva in guinea pig lung, whereas none were detected in adult male worm or adult female worm, except for the female reproductive organs. the ovary extracts we ... | 2007 | 17391851 |
| modification of cepsilon mrna expression by ebv-encoded latent membrane protein 1. | to evaluate the effect of expression of latent membrane protein (lmp) 1 encoded by epstein-barr virus (ebv) on cepsilon mrna expression, mrna levels were examined by rt-pcr or northern blot analysis upon transient transfection of lmp1 in the splenocytes derived from brown-norway rats with or without immunization with 2,4-dinitrophenyl-conjugated ascaris suum antigen. splenocytes were transfected with lmp1 expression vector, psg5-lmp1, using lipofection method. cepsilon mrna levels were considera ... | 2006 | 17392589 |
| concurrent infection with trichinella spiralis and other helminths in pigs. | the aim of this study was to investigate possible influence of different helmintosis in the development of trichinella spiralis in experimental infected pigs. forty-two iberian pigs were allocated to six groups. three groups were single inoculated with ascaris suum, metastrongylus apri or t. spiralis, respectively. two groups were co-infected with t. spiralis and a. suum or t. spiralis and m. apri, respectively, while the last group included uninfected control pigs. clinical signs were only obse ... | 2007 | 17400390 |
| a comparison of the efficacy of two ivermectin formulations against larval and adult ascaris suum and oesophagostomum dentatum in experimentally infected pigs. | a study was conducted to evaluate and compare the efficacy of two injectable formulations of ivermectin (ivm-1 and ivm-2) at a dose rate of 0.3 mg/kg bodyweight versus placebo in the treatment and control of larval and adult stages of ascaris suum and oesophagostomum spp. in experimentally infected pigs. seventy helminth free pigs were allocated on a liveweight basis to 7 groups each comprising 10 pigs (a-g). group a served as an untreated control group. groups b and c were used to investigate t ... | 2007 | 17418952 |
| saskatchewan. spurious ascaris suum infection in lambs. | | 1989 | 17423463 |
| l-454,560, a potent and selective pde4 inhibitor with in vivo efficacy in animal models of asthma and cognition. | type 4 phosphodiesterases (pde4) inhibitors are emerging therapeutics in the treatment of a number of chronic disorders including asthma, chronic obstructive pulmonary disease (copd) and cognitive disorders. this study delineates the preclinical profile of l-454,560, which is a potent, competitive and preferential inhibitor of pde4a, 4b, and 4d with ic50 values of 1.6, 0.5 and 1.2 nm, respectively. in contrast to the exclusive binding of cilomilast and the preferential binding of roflumilast to ... | 2007 | 17428447 |
| peptide products of the afp-6 gene of the nematode ascaris suum have different biological actions. | matrix-assisted laser desorption/ionization time-of-flight and tandem time-of-flight (maldi-tof and maldi-tof/tof) mass spectrometry were used to sequence and localize three novel, related neuropeptides in the nervous system of the nematode ascaris suum, amrnalvrfamide (af21), ngapqpfvrfamide (af22), and sgmrnalvrfamide (af23). the amino acid sequences were used to clone a novel neuropeptide gene (afp-6) that encodes a precursor bearing a single copy of each of the peptides. in situ hybridizatio ... | 2007 | 17436302 |
| decrease of time for pathogen inactivation in alkaline disinfection systems using pressure. | from field studies conducted by tulane university (new orleans, louisiana), efficiency of advanced alkaline disinfection in closed systems was found to depend on ammonia concentration, ph, exposure time, temperature, total solids content, pretreatment storage time, and mixing effectiveness. in this study of a closed alkaline system, an additional pathogen stressor pressure was tested. the effect of the alkaline dosing has been assessed for dewatered raw and aerobically and anaerobically digested ... | 2007 | 17489273 |
| modulation of anaphylaxis by helminth-derived products in animal models. | helminths have a profound immunomodulatory effect upon the inductive and effector phases of inflammatory responses, including allergy. several animal models of anaphylaxis have been established to investigate the mechanisms by which helminth infections or helminth-derived products interfere with the onset of allergic reactions. the focus of our studies was the immunosuppression induced by the intestinal roundworm ascaris suum in the production of anaphylactic antibodies and the development of lu ... | 2007 | 17504662 |
| the nematode neuropeptide, af2 (kheylrf-nh2), increases voltage-activated calcium currents in ascaris suum muscle. | resistance to all the classes of anti-nematodal drugs like the benzimidazoles, cholinergic agonists and avermectins, has now been recorded in parasites of animals and/or humans. the development of novel anthelmintics is an urgent and imperative need. receptors of nematode neuropeptides have been suggested to be suitable target sites for novel anthelmintic drugs. | 2007 | 17519945 |
| pulmonary computed tomography findings of visceral larva migrans caused by ascaris suum. | to retrospectively evaluate the computed tomography (ct) findings of pulmonary involvement in patients with visceral larva migrans caused by ascaris suum. | 2007 | 17538287 |
| [a case of visceral larva migrans due to toxocara canis showing varied manifestations]. | a 21-year-old woman admitted for a low-grade fever, dry cough, polyarthralgia, and general fatigue was found to have elevateal accustomed to eating raw meat and cattle liver peripheral blood eosinophil counts and serum ige. chest x-ray imaging and computed tomography (ct) showed multiple small nodules in both lung fields. a multiple dot-elisa assay of her serum showed that she had visceral larva migrans caused by ascaris suum or toxocara canis. following treatment with albendazole, she developed ... | 2007 | 17564121 |
| ultrastructural changes in ascaris suum after oxibendazole treatment. | the effect of oxibendazole treatment on adult ascaris suum was assessed using scanning and transmission electron microscopy. the changes included collapse of the lip papillae, rupture of buccal cavity cuticle, prolapse of the pharynx, degeneration of epithelial cells and erosion of microvilli. theses changes may lead to impaired digestion and absorption of nutrients and cause cellular autolysis, resulting in the death of the worm. | 1998 | 17580993 |
| infection with parasitic nematodes confounds vaccination efficacy. | t helper (th) cells produce signature cytokine patterns, induced largely by intracellular versus extracellular pathogens that provide the cellular and molecular basis for counter regulatory expression of protective immunity during concurrent infections. the production of il-12 and ifn-gamma, for example, resulting from exposure to many bacterial, viral, and protozoan pathogens is responsible for th1-derived protective responses that also can inhibit development of th2-cells expressing il-4-depen ... | 2007 | 17587500 |
| mutagenicity of ascaris chymotrypsin inhibitor in germ cells of mice. | chymotrypsin inhibitor isolated from ascaris suum (achi) was tested for the induction of dominant lethal mutations in male mice. dominant lethal effects of achi for the main stages of germ cell development were analyzed by mating at specific time points after dosing. two groups of adult balb/c males received 24 or 40 mg per kilogram body weight (bw) per day intraperitoneal (ip) injection of achi in sterile phosphate-buffered saline (pbs) for five consecutive days (subacute exposure). males from ... | 2008 | 17674131 |
| a comparison of modifications of the mcmaster method for the enumeration of ascaris suum eggs in pig faecal samples. | the comparative efficacies of seven published mcmaster method modifications for faecal egg counting were evaluated on pig faecal samples containing ascaris suum eggs. comparisons were made as to the number of samples found to be positive by each of the methods, the total egg counts per gram (epg) of faeces, the variations in epg obtained in the samples examined, and the ease of use of each of the methods. each method was evaluated after the examination of 30 samples of faeces. the positive sampl ... | 2007 | 17703889 |
| inhibitory receptor gp49b regulates eosinophil infiltration during allergic inflammation. | gp49b, an ig-like receptor, negatively regulates the activity of mast cells and neutrophils through cytoplasmic immunoreceptor tyrosine-based inhibition motifs. to characterize the role of gp49b further in vivo, gp49b-deficient mice were tested in two allergic models. responses to ragweed (rw) challenge in the lung and conjunctiva were assessed in models of allergic inflammation and during an infection with parasitic larvae of the nematode ascaris suum. infiltration by inflammatory cells into th ... | 2007 | 17761953 |
| inactivation of ascaris suum in a biodrying compost system. | pathogen contamination of waterways is a serious concern in dairy farming areas where livestock waste is applied to agricultural fields. as an alternative, a biodrying composting system dries collected livestock waste, reduces the strong odors, and has been proposed as a means of reducing, and even eliminating pathogens present in the waste. therefore, the survival of pathogens in a biodrying composting system was investigated. dairy farm livestock waste was piled in a biodrying storage shed whe ... | 2007 | 17766833 |
| ascaris suum in sheep. | | 1914 | 17800310 |
| fmrfamide-like peptides encoded on the flp-18 precursor gene activate two isoforms of the orphan caenorhabditis elegans g-protein-coupled receptor y58g8a.4 heterologously expressed in mammalian cells. | two alternatively spliced variants of an orphan caenorhabditis elegans g-protein-coupled receptors (gpcrs; y58g8a.4a and y58g8a.4b) were cloned and functionally expressed in chinese hamster ovary (cho) cells. the y58g8a.4a and y58g8a.4b proteins (397 and 433 amino acid residues, respectively) differ both in amino acid sequence and length of the c-terminal tail of the receptor. a calcium mobilization assay was used as a read-out for receptor function. both receptors were activated, with nanomolar ... | 2008 | 17879267 |
| parasite-specific il-4 responses in ascaris suum and trichuris suis-infected pigs evaluated by elispot. | the objective of the present study was to develop an elispot method to measure parasite-specific il-4 producing cells during experimental ascaris suum and trichuris suis infections in pigs. in many experimental settings it is useful to be able to measure changes in specifically induced cytokines over time at post-mrna level; in particular, specific measurement of il-4 is important for studies on nematodes due to the key function of il-4 in driving the th2 response. two separate experiments were ... | 2007 | 17883456 |
| [effects of some species of hydrocoles on the development of ascarid eggs]. | the study established that aquatic plants differently affected the development of ascarid eggs. some species of algae exerted a damaging effect on ascarid eggs only at early stages of embryogenesis while others did at its late stages, but some species did not produce any noticeable effect on the development of the eggs. a great difference was found in the development of ascarid eggs in different seasons. gas exchange conditions were ascertained to affect the development of ascarid eggs. analysis ... | 2007 | 17912827 |
| change of subunit composition of mitochondrial complex ii (succinate-ubiquinone reductase/quinol-fumarate reductase) in ascaris suum during the migration in the experimental host. | the mitochondrial metabolic pathway of the parasitic nematode ascaris suum changes dramatically during its life cycle, to adapt to changes in the environmental oxygen concentration. we previously showed that a. suum mitochondria express stage-specific isoforms of complex ii (succinate-ubiquinone reductase: sqr/quinol-fumarate reductase: qfr). the flavoprotein (fp) and small subunit of cytochrome b (cybs) in adult complex ii differ from those of infective third stage larval (l3) complex ii. howev ... | 2008 | 17933581 |
| decursin and decursinol angelate selectively inhibit nadh-fumarate reductase of ascaris suum. | nadh-fumarate reductase (nfrd) is a key enzyme in many anaerobic helminths. decursin and decursinol angelate have been isolated from the roots of angelica gigas nakai (apiaceae) as nfrd inhibitors. they inhibited ascaris suum nfrd with ic (50) values of 1.1 and 2.7 microm, respectively. their target is the electron transport enzyme complex i. since the inhibitory activities of decursin against bovine heart complexes are weak, it is a selective inhibitor of the nematode complex i. in contrast, de ... | 2007 | 17948169 |
| a mouse fcgamma-fcepsilon protein that inhibits mast cells through activation of fcgammariib, sh2 domain-containing inositol phosphatase 1, and sh2 domain-containing protein tyrosine phosphatases. | a human fcgamma-fcepsilon fusion protein (ge2) designed to inhibit fcepsilonri signaling by coaggregating fcepsilonri with the inhibitory receptor fcgammariib has been shown to inhibit mast cell activation and block cutaneous anaphylaxis. a critical issue remained as to whether the mechanism of ge2 inhibition is competition for ige binding or inhibitory signaling through fcgammariib. | 2008 | 17949802 |
| helminths of wild boar in the isolated population close to the northern border of its habitat area. | one hundred wild boars (sus scrofa) from a geographically isolated population on the island saaremaa of western estonia were examined for visceral helminths. seven helminth species, metastrongylus pudendotectus, m. salmi, m. elongatus, ascaris suum, trichuris suis, dicrocoelium dendriticum and taenia hydatigena larva, were found. the predominant helminths discovered were lung nematodes (prevalence 82%, mean intensity 96.2 per animal). a significant negative correlation was observed between the w ... | 2007 | 17964726 |
| western blot antibody determination in sera from patients diagnosed with anisakis sensitization with different antigenic fractions of anisakis simplex purified by affinity chromatography. | using western blot techniques, the specificities of crude and purified (pak and pas) anisakis simplex antigens were compared against 24 sera from patients diagnosed with anisakis sensitization. all patients recognized a 60 kda protein against the a. simplex crude extract, while 37.5% and 12.5% reacted with proteins of 40 and 25 kda, respectively, when igg was tested. in the case of ige determination, 41.6% of sera were negative, while 12.5% and 20.8% appeared to cross-react against toxocara cani ... | 2007 | 17974043 |