| chromatographic studies of mitomycin c degradation in albumin microspheres. | serum albumins and polylactic acid (pla) have been used as bioerodable polymers in the preparation of drug-containing microspheres for parenteral drug delivery. the albumin microsphere may be prepared via either chemical cross-linking or heat denaturation of the protein. heat-denatured albumin microspheres containing mitomycin c (mmc) have been used in pre-clinical and clinical investigations. due to the high reactivity of mmc as a bifunctional alkylating agent, a study on the stability of mmc i ... | 1988 | 3148622 |
| characterization of the priming effects of human granulocyte-macrophage colony-stimulating factor on human neutrophil leukotriene synthesis. | human granulocyte-macrophage colony-stimulating factor (gm-csf) is an in vitro and in vivo stimulator of human bone marrow myelomonocytic precursor cells and mature granulocyte and macrophage effector cells. we have compared the effect of gm-csf on the synthesis of 5-lipoxygenase products induced by the chemotactic peptide fmet-leu-phe and the calcium ionophore a23187 in human neutrophils. although gm-csf alone did not stimulate detectable synthesis of products of the 5-lipoxygenase pathway, pre ... | 1988 | 3148964 |
| [lysosomal enzyme activity in the nerve tissue of various species of animals after administration of a neurotoxic pesticide aphos]. | | 1988 | 3149613 |
| production of prostaglandins and hete-compounds by isolated-cultivated bovine aortic endothelial cells. | the main product of aa conversion by baec is pgi2. other pgs were synthesized by confluent cells only in traces with exception of a hht-like compound which has still to be characterized in more details. a significant release of hete was only detected when baec were destroyed. under these conditions about 1 pmole/10(5) cells 15- and 12 hete were released by baec. taken into account the amounts of hete produced by endothelial cells as well as the partially contradictory activities of 12- and 15-he ... | 1988 | 3150276 |
| sodium valproate and chlordiazepoxide in the elevated plus-maze test of anxiety in the rat. | the effects of sodium valproate (25-200 mg/kg i.p.) were investigated in the elevated plus-maze test of anxiety in the rat. no single dose significantly increased the percentage of entries made onto the open arms. chlordiazepoxide (5 mg/kg) both acutely and after 5 days of administration significantly increased the percentage of entries onto open arms and this anxiolytic effect was neither potentiated, nor antagonised, by sodium valproate (25-200 mg/kg). chlordiazepoxide also increased the total ... | 1988 | 3151011 |
| relevant problems to the epidemiology of mansonic schistosomiasis. | | 1987 | 3151110 |
| influenza viruses as lymphocyte mitogens. i. b cell mitogenesis by influenza a viruses of the h2 and h6 subtypes is controlled by the i-e/c subregion of the major histocompatibility complex. | influenza a viruses of the h2, h3, and h6 subtypes function as t cell-independent b cell mitogens for lymphocytes from balb/c mice. lymphocytes from c57bl/10 mice, however, undergo mitogenesis only in response to h3 viruses. the failure of c57bl/10 lymphocytes to respond to h2 and h6 viruses was shown not to reflect a difference in dose-response profile or kinetics of the response, nor was it due to the activity of suppressor t cells. experiments with congenic and recombinant strains of mice est ... | 1985 | 3155540 |
| non-immune igg f(ab')2 binding to group c and g streptococci is mediated by structures on gamma chains. | the present investigation was designed to determine whether the heavy or the light immunoglobulin chain is involved in the non-immune binding of igg f(ab')2 fragments to specific surface receptors on human group c and g streptococci. purified human polyclonal igg was mildly reduced with dithiothreitol and alkylated with iodoacetamide. light (l) and heavy (h) chains were separated. intact igg and purified l and h chains of polyclonal immunoglobulin g were tested in an inhibition assay for non-imm ... | 1985 | 3156400 |
| [inhibitory effects of bisphosphonates on the resorption of alveolar bone in rats]. | | 1985 | 3161960 |
| [levels of prostaglandin e1 and f2 alpha in the dynamics of toxic-infectious shock induced by yersinia pestis]. | murine toxin of yersinia pestis when injected in the rat tail vein (ld50) caused pronounced alterations in pge1 and pgf2 alpha content in different tissues (lung, heart, spleen, liver, kidney, small intestine) and blood. heat-inactivated toxin has been shown to have the same effects as the intact toxin preparation. the changes in pg content are, probably, due to the lipopolysaccharide component of both preparations. the differences in metabolic effects between yersinia pestis endotoxin and lipop ... | 1988 | 3162386 |
| luteolytic potency of 16-phenoxy-derivatives of prostaglandin f2 alpha. | the binding of 16-phenoxy derivatives of prostaglandin (pg) f2 alpha to rat luteal membranes, and also their abortifacient potency in pregnant rats, have been studied. competitive binding studies with various pg-analogues were performed in ovaries of juvenile rats pretreated with pmsg and hcg, and in parallel studies the abortifacient potency of these substances was tested in pregnant rats. it was observed that this class of derivatives bound to the pgf2 alpha receptor as well as, or even better ... | 1988 | 3162421 |
| mechanisms of conversion of xanthine dehydrogenase to xanthine oxidase in ischemic rat liver and kidney. | previous studies have proposed and supported a role for the proteolytic, irreversible conversion of xanthine dehydrogenase to xanthine oxidase (xo) in postischemic injury in a wide variety of organs. a second mechanism of conversion, due to sulfhydryl modification and reversible with dithiothreitol (dtt), is potentially important but has not been well investigated. in this study rat liver and kidney were found to produce significant amounts of dtt-reversible xo during normothermic global ischemi ... | 1988 | 3163235 |
| a double-blind comparison of ibuprofen, placebo and ibuprofen with meptazinol in soft tissue rheumatism. | the administration of ibuprofen potentiates and prolongs the analgesic effect of meptazinol when the two drugs are given simultaneously to mice. a double-blind three-way crossover study of placebo, ibuprofen (1600 mg/day) and ibuprofen (1600 mg/day) plus meptazinol (400 mg/day) was carried out in 45 patients with soft tissue rheumatism to see if the same potentiation could be demonstrated in man. treatment order was randomized and each regimen was given for 2 weeks preceded by 1 week on paraceta ... | 1988 | 3163538 |
| jamshidi needle biopsy for diagnosis of bone lesions in small animals. | sixty-two bone lesions in 59 small animals were biopsied using a jamshidi-type biopsy needle. in all instances, the jamshidi needle biopsy diagnosis was confirmed or disputed by results of histologic examination of specimens obtained by surgical resection or by amputation or at necropsy. in 57 of 62 bone lesions, biopsy resulted in an accurate diagnosis of tumor vs nontumor, yielding an accuracy rate of 91.9%. the specific tumor type or lesion type was identified accurately by jamshidi needle bi ... | 1988 | 3165373 |
| [an immunocytochemical study on the prostaglandin e2 localization in human gastric mucosa]. | | 1988 | 3165480 |
| production of tumor necrosis factor in nude mice by muramyl peptides associated with bacterial vaccines. | gram-negative vaccines can elicit the production of tumor necrosis factor (tnf) in mice primed by muramyl dipeptide (mdp) or by its lipophilic derivative mdp-dipalmitoyl glycerol (mdp-gdp). in mice pretreated with mdp and particularly with mdp-gdp, bordetella pertussis vaccine was shown to be more effective than typhoid vaccine. the time course of tnf production in the blood did not indicate any difference between the effect of mdp or of mdp-gdp. in both cases the cytotoxic activity reached maxi ... | 1988 | 3167834 |
| a new approach to the quality control of steroid receptor assays. | lyophilized cytosols prepared from calf uterus and human breast tumor tissue are commonly used to assess the reliability of routine steroid receptor assays. however, preanalytical error (sample preparation, storage, homogenization) cannot be detected in this way. participating laboratories were asked to mail us all their receptor results obtained over a four-month interval, and to include some information about the patients involved (age, menopausal status, nodal status). after verifying the hom ... | 1988 | 3168219 |
| [effect of the entomopathogenic fungus penicillium funiculosum on the reproduction of the causative agent of plague in the body of fleas]. | | 1988 | 3173243 |
| [qualitative and quantitative changes in the phospholipid composition in experimental brain edema and during treatment with antiedema preparations]. | | 1988 | 3174245 |
| receptor-like function of heparin in the binding and uptake of neutral lipids. | molecular mechanisms regulating the binding, amphipathic stabilization, and metabolism of the major neutral lipids (e.g., cholesteryl esters, triglycerides, and fatty acids) are well studied, but the details of their movement from a binding compartment to a metabolic compartment deserve further attention. since all neutral lipids must cross hydrophilic segments of plasma membranes during such movement, we postulate that a critical receptor-like site exists on the plasma membrane to mediate a ste ... | 1988 | 3174644 |
| funding of project to map human genes sparks debate. | | 1988 | 3177203 |
| phasic activity of human dorsal foot veins. | phasic contractions of the dorsal foot veins were detected in 7 male subjects, aged 23 to 49 years, by means of a linear variable differential transformer (lvdt) after congesting the veins by a pressure of 6.7 kpa (50 torr). this phasic activity (pa) had a frequency of 3 to 7 contractions per minute and led to a periodic diminution of the diameter of the distended veins by 12 to 44%. using a double lvdt, the pa was shown to represent peristaltic waves travelling along the vessel wall concomitant ... | 1988 | 3178793 |
| [identification of the principal species of the genus serratia by a simplified system]. | a reduced identification system for the genus serratia is proposed. the usefulness of this system was shown by identification of 720 strains from clinical specimens or natural environment. computation of "diagnosis ability coefficient" and principal component analysis made it possible to reduce this identification system to 9 tests. numerical taxonomy displayed the 9 recognized species. | 1988 | 3179060 |
| localization of the human dopamine beta hydroxylase (dbh) gene to chromosome 9q34. | a human cdna clone for dopamine beta hydroxylase (dbh) has been isolated from a phaeochromocytoma library. in situ hybridization of this probe to replication-banded chromosomes has localized the gene to chromosome 9q34. the structural gene for the enzyme is therefore close to the abo blood group locus. this suggests that the previously described activity variation in levels of serum dbh may reflect alterations in either the structure or regulation of the dbh coding sequences. both biochemical an ... | 1988 | 3180847 |
| some chemical properties of maitotoxin, a putative calcium channel agonist isolated from a marine dinoflagellate. | maitotoxion, a putative ca2+ channel agonist, was isolated from cultures of the marine dinoflagellate gambierdiscus toxicus as a colorless amorphous solid. the toxin reacted positively to dragendorff's reagent but not to ninhydrin reagent. the mouse lethality of maitotoxin determined by intraperitoneal injection was 0.13 micrograms/kg. chemical features of the toxin were elucidated mainly by various spectroscopic measurements. the molecular weight of maitotoxin as a disodium salt was estimated t ... | 1988 | 3182760 |
| some chemical properties of maitotoxin, a putative calcium channel agonist isolated from a marine dinoflagellate. | maitotoxion, a putative ca2+ channel agonist, was isolated from cultures of the marine dinoflagellate gambierdiscus toxicus as a colorless amorphous solid. the toxin reacted positively to dragendorff's reagent but not to ninhydrin reagent. the mouse lethality of maitotoxin determined by intraperitoneal injection was 0.13 micrograms/kg. chemical features of the toxin were elucidated mainly by various spectroscopic measurements. the molecular weight of maitotoxin as a disodium salt was estimated t ... | 1988 | 3182760 |
| a role for glyceraldehyde-3-phosphate dehydrogenase in the development of thermotolerance in xenopus laevis embryos. | during heat shock, xenopus laevis embryos exhibit an increase in the rate of accumulation of lactate and a loss of atp relative to non-heat-shocked control embryos. these results suggest that heat shock stimulates a shift in energy metabolism to anaerobic glycolysis while at the same time causing an increase in the demand for atp. we have evidence indicating that the embryo may meet such demands placed on it by increasing the levels of some glycolytic enzymes. in this report, we show that heat s ... | 1988 | 3182940 |
| organization of a complex movement: fixed and variable components of the cockroach escape behavior. | the escape behavior of the cockroach periplaneta americana was studied by means of high speed filming (250 frames/s) and a computer-graphical analysis of the body and leg movements. the results are as follows: 1. the behavior begins with pure rotation of the body about the posteriorly located cerci, followed by rotation plus forward translation, and finally pure translation (figs. 1, 2). 2. a consistent inter-leg coordination is used for the entire duration of the turn (fig. 3a). at the start of ... | 1988 | 3184004 |
| [effect of the induction of liver cytochrome p-450 by a chemically inert fluorocarbon on the reactions of immediate and delayed hypersensitivity]. | the effect of chemically inert perfluorodecalin (pfd) on the development of hypersensitive reactions of immediate and delayed types in guinea pigs and mice was studied. it was found that administration of pfd to animals both before sensitization (mice) and during the development of sensitization (guinea pigs) reduced the degree of the both types of hypersensitivity. | 1988 | 3191983 |
| effect of bromoethylamine hydrobromide on systemic acid-base balance. | intravenous administration of bromoethylamine hydrobromide (bea) has been shown to induce papillary necrosis of the kidney. we used this model to clarify the role of the medullary structure in acid-base homeostasis. bea (25 mg) or vehicle was injected to male sprague-dawley rats. blood specimens and 24 hr urine were collected once a week totaling 4 weeks. blood bicarbonate significantly decreased in bea treated rats with no change in plasma creatinine or creatinine clearance at 3 and 4 weeks, we ... | 1988 | 3194903 |
| treatment of fasciola hepatica infection in horses with triclabendazole. | thirteen thoroughbred foals with an adult infection and a presumed immature infection with fasciola hepatica were treated with 12 mg triclabendazole/kg bodyweight. the absence of eggs from samples of faeces examined at intervals up to 110 days after treatment showed that all the animals were cured. in a second trial the same dose of triclabendazole cured 11 of 12 foals whereas nitroxynil at 7 mg/kg cured only six of 12 foals. no side effects were observed after treatment. | 1988 | 3195012 |
| mutations in the hemagglutinin gene associated with influenza virus resistance to norakin. | the nucleotide sequences of the hemagglutinin genes of four norakin-resistant mutants of influenza a/fpv/weybridge were determined and compared to the wild-type hemagglutinin. all mutants show one or two amino acid substitutions which are discussed to destabilise the ph 7 conformation of hemagglutinin. | 1988 | 3196166 |
| seronegative virus carriers in the infection of rabbits with human t lymphotropic virus type i. | six htlv-i-transformed t cell lines were prepared from pbl of three rabbits each of b/j and chbb:hm strains, and were inoculated into newborn rabbits of these two strains, and of their f1 hybrid. none of three b/j cell lines induced anti-htlv-i antibody response in newborn b/j rabbits, whereas all three chbb:hm cell lines did induce a response in newborn chbb:hm rabbits. these b/j cell lines however could induce antibody response in adult b/j as well as newborn chbb:hm rabbits, and a chbb:hm cel ... | 1988 | 3199075 |
| seronegative virus carriers in the infection of rabbits with human t lymphotropic virus type i. | six htlv-i-transformed t cell lines were prepared from pbl of three rabbits each of b/j and chbb:hm strains, and were inoculated into newborn rabbits of these two strains, and of their f1 hybrid. none of three b/j cell lines induced anti-htlv-i antibody response in newborn b/j rabbits, whereas all three chbb:hm cell lines did induce a response in newborn chbb:hm rabbits. these b/j cell lines however could induce antibody response in adult b/j as well as newborn chbb:hm rabbits, and a chbb:hm cel ... | 1988 | 3199075 |
| complex organization of the length heterogeneous 5' external spacer of mung bean (vigna radiata) ribosomal dna. | restriction enzyme analysis and cloning of the 18s, 5.8s, and 25s ribosomal rna genes (rdna) of the mung bean (vigna radiata = phaseolus aureus) reveal length heterogeneity in the repeating units (10-11 kbp) localized within two different regions in the ribosomal spacer. the 1.5-2.0 kbp region flanking the 3' end of the 25s rrna contains various numbers (8-10) of tandemly arranged 180 bp subrepeats. after dna sequencing a complex organized length heterogeneous 5' external spacer built up of diff ... | 1988 | 3203889 |
| double myelination of axons in the sympathetic nervous system of the mouse. ii. mechanisms of formation. | the phenomenon termed 'double myelination', present in sympathetic nerve of normal adult rats and mice, comprises regions of a myelinated axon which are concentrically ensheathed by additional (outer) myelinating schwann cells. evidence has been presented that in some instances the outer schwann cell fails to make contact with an axon, yet its myelin sheath characteristically remains ultrastructurally intact. the present study has sought to identify and analyse configurations intermediate betwee ... | 1988 | 3204414 |
| diseases of winter housed sheep. | | 1988 | 3206810 |
| [changes in the "latent" virulence of a vaccinal strain of yersinia pestis multiplying within macrophages]. | the multiplication of y. pestis vaccinal strain inside peritoneal macrophages of guinea pigs and white mice in vitro leads to an essential increase in its latent virulence. this effect is most pronounced when guinea pig macrophages are used. changes in the latent virulence of y. pestis vaccinal strains, occurring in the process of their passage inside macrophages in vitro, correlate with those observed in vivo, i.e. in animal experiments. | 1988 | 3206961 |
| [bactericidal effect of tween 80 on mycobacteria]. | | 1988 | 3210511 |
| effect of aminooxyacetic acid on the release of preloaded [3h]gaba and radioactive metabolites from slices of developing mouse brain. | the release of [3h]gamma-aminobutyric acid (gaba) and its radioactive metabolites from slices of the cerebral cortex, cerebellum, striatum and brain stem of developing and adult mice was studied. the slices were incubated and superfused in the absence and presence of the gaba aminotransferase (gaba-t) inhibitor aminooxyacetic acid (aoaa). exposure to 100 microm aoaa totally inhibited gaba-t and all radioactivity released from slices was in authentic gaba. in studies on developing brain the 10-mi ... | 1988 | 3216949 |
| [prospects for the use of monoclonal antibodies in yersinia pestis]. | industrial production of plague monoclonal antibodies in mice balb/c was shown possible. precipitation of the immunoglobulins with caprylic acid provided the highest purity of the preparations. maintenance of the specific activity by the monoclonal antibodies was stable. the immunoenzymatic conjugate and erythrocytic diagnosticum on the basis of the monoclonal antibodies are sensitive, specific and may be used in antiplague practice. | 1988 | 3218993 |
| morphological variation of echinococcus granulosus protoscoleces from hydatid cysts of human and various domestic animals in jordan. | | 1988 | 3220653 |
| observations on commensal rats and their status to plague in bombay. | the distribution pattern of various types of commensal rodents in bombay city reveals that bandicota bengalensis constitutes the predominant commensal rodent species followed by r. rattus and rattus norvegicus. apart from these three types, bandicota indica, m. musculus and an insectivore (suncus murinus) are the three species of commensal small mammals that are frequently encountered in or near human habitations. these small mammals are prevalent throughout the year and their percentage distrib ... | 1988 | 3221091 |
| [behavioral, morphologic and biochemical correlates of the early effect of a neural transplant into the damaged brain of adult rats]. | complex study was carried out of the behavioural, morphological and neurobiochemical parameters of the effect of an embryonal neural transplant of the amygdala in the first weeks after its introduction into the area of the left amygdala of adult rats-recipients. the neural transplant promoted normalization of animals food search, disturbed by operative interference. the amygdalar embryonal tissue lowered the proliferative glia reaction, produced by the brain lesion, and intensified the nervous t ... | 1988 | 3223076 |
| beneficial effect of omeprazole in a patient with severe bronchial asthma and gastro-oesophageal reflux. | a 25-yr-old man suffered from severe nocturnal asthma, which was shown to be provoked by pathological gastro-oesophageal reflux. a dramatic, immediate improvement of his pulmonary condition was achieved by treatment with omeprazole after failure of other therapeutic measures, including high doses of ranitidine. | 1988 | 3224694 |
| identification of a yersinia pestis-specific dna probe with potential for use in plague surveillance. | a 900-base-pair dna fragment derived from a 9.5-kilobase plasmid in yersinia pestis hybridized specifically with y. pestis dna. we demonstrated the feasibility of using this dna fragment to detect plague bacilli directly in fleas, suggesting that this y. pestis-specific dna probe may be used for plague surveillance in the field. additional applications for this dna probe may include plague diagnosis and pathogenesis research. | 1988 | 3230131 |
| role of renal prostaglandins in bile-induced diuresis in the dog. | to clarify the possible role of renal prostaglandins (pgs) in the phenomenon of bile-induced diuresis in the dog, we studied the effect of in situ unilateral infusion of bile on kidney function and pgs excretion, before and after pg-synthetase inhibitor administration in anesthetized dogs. the contralateral intact kidney served as control. in the first group of 6 dogs, infusion of bile diluted 1:20 resulted in a significant increase in urinary flow (117%; p less than 0.05), sodium (61%; p less t ... | 1988 | 3231185 |
| importance of a 3-o-sulfate group in a heparin pentasaccharide for antithrombotic activity. | previous theoretical and experimental evidence led to the formulation of a specific pentasaccharide structure which represents the site in heparin for binding to antithrombin iii. this pentasaccharide was subsequently synthesized. a pentasaccharide of the same structure but lacking only the sulfate group on the hydroxyl group of the middle glucosamine (position c-3) was also synthesized to test the structure - activity relationships. previous biochemical studies showed the 3-o-desulfated pentasa ... | 1988 | 3232125 |
| examining the impact of different health levels and management strategies on dairy heifer production through computer simulation. | | 1988 | 3232667 |
| magnesium salts exert direct vasodilator effects on rat cremaster muscle microcirculation. | in vivo microscopic observations were made on arterioles and venules (10-20 microns i.d.) of the rat cremaster muscle circulation, using an image-splitting eyepiece and high-resolution tv image-intensification, in order to determine the microvascular effects of inorganic and organic magnesium (mg) salts. mgso4, mgcl2, mg aspartate hcl, and mg acetate were administered perivascularly and intravenously. topical application (10(-2) to 10(1) mumol) and intravenous (i.v.) administration (5-400 mumol/ ... | 1988 | 3233048 |
| effects of acute doses of ethanol administered at pre-implantation stages on fetal development in the mouse. | the objective of the present study was to investigate the effects of maternal administration of acute doses of alcohol at preimplantation stages of pregnancy on the fetus and fetal membranes. single doses of 0.02 ml and 0.03 ml/g body wt. of 25% v/v of absolute alcohol in saline were administered to groups of mf1 mice on one of days 1-6 of gestation. controls were either pair-fed and pair-watered or saline-treated. fetuses and placentae were collected on day 15. resorption and fetal death were i ... | 1988 | 3234238 |
| periodicity coding in the inferior colliculus of the cat. ii. topographical organization. | 1. the topographical distributions of single-unit and multiple-unit responses to amplitude-modulated tones--and to other relevant parameters of simple tonal stimuli--were defined across the main frequency representational gradient and within narrow frequency ranges represented in "frequency band laminae" in the principal midbrain auditory nucleus, the central nucleus of the inferior colliculus (icc), in adult, barbiturate-anesthetized cats. 2. responses to amplitude-modulated tones with the carr ... | 1988 | 3236053 |
| periodicity coding in the inferior colliculus of the cat. ii. topographical organization. | 1. the topographical distributions of single-unit and multiple-unit responses to amplitude-modulated tones--and to other relevant parameters of simple tonal stimuli--were defined across the main frequency representational gradient and within narrow frequency ranges represented in "frequency band laminae" in the principal midbrain auditory nucleus, the central nucleus of the inferior colliculus (icc), in adult, barbiturate-anesthetized cats. 2. responses to amplitude-modulated tones with the carr ... | 1988 | 3236053 |
| dimetridazole residues in pork tissue. i. assay by liquid chromatography with electrochemical detector. | a liquid chromatographic (lc) method with electrochemical detection in the reductive mode was developed for the quantitative determination of dimetridazole (dmz) and its major metabolite (hmmni) at residue levels in pork tissue. for blood plasma, a sample is precipitated with 2 volumes of acetonitrile and centrifuged, and a diluted aliquot of the supernatant liquid is chromatographed. for muscle, a 10 g sample is extracted 3 times with dichloromethane. after evaporation of the combined extracts, ... | 1988 | 3240970 |
| the effect of methylxanthines on morphine analgesia in mice and rats. | the influence of various doses of caffeine and theophylline on analgesic effect of morphine was tested in mice and rats in the hot plate and tail immersion tests. caffeine in rats potentiated the morphine analgesia in both tests in a dose-dependent manner. in mice the caffeine-morphine interaction was biphasic: lower caffeine doses (10 and 25 mg/kg) inhibited, while higher (75 and 100 mg/kg) potentiated the analgesic effects of morphine. theophylline interacted with morphine in a manner similar ... | 1988 | 3241763 |
| [study of the virulence plasmids of 100 strains of yersinia pestis isolated in madagascar]. | | 1988 | 3242429 |
| [9,12,13-trihydroxy-10(e)-octadecenoic and 9,12,13-trihydroxy-10,11-epoxy-octadecanoic acids--new antistress substances isolated from licorice]. | | 1988 | 3243940 |
| purification, partial characterization and biological effects of the xtc mesoderm-inducing factor. | the mesoderm of xenopus laevis is formed through an inductive interaction in which a signal from the vegetal hemisphere of the blastula acts on overlying animal pole cells. we have recently reported that the xenopus xtc cell line secretes a mesoderm-inducing factor (mif) which may resemble the natural signal. in this paper, we describe the purification and biological effects of xtc-mif. xtc-mif is a hydrophobic protein with an isoelectric point of 7.8 and an apparent relative molecular mass (mr) ... | 1988 | 3246228 |
| purification, partial characterization and biological effects of the xtc mesoderm-inducing factor. | the mesoderm of xenopus laevis is formed through an inductive interaction in which a signal from the vegetal hemisphere of the blastula acts on overlying animal pole cells. we have recently reported that the xenopus xtc cell line secretes a mesoderm-inducing factor (mif) which may resemble the natural signal. in this paper, we describe the purification and biological effects of xtc-mif. xtc-mif is a hydrophobic protein with an isoelectric point of 7.8 and an apparent relative molecular mass (mr) ... | 1988 | 3246228 |
| [studies on the nontuberculous lung mycobacteriosis in japan (report of the study in the year 1986 of the mycobacteriosis research group of the japanese national chest hospitals)--the prevalence rate of nontuberculous lung mycobacteriosis in japan is gradually increasing since 1984--the mycobacteriosis research group of the japanese national chest hospitals]. | | 1988 | 3249525 |
| [studies on the nontuberculous lung mycobacteriosis in japan (report of the study in the year 1986 of the mycobacteriosis research group of the japanese national chest hospitals)--the prevalence rate of nontuberculous lung mycobacteriosis in japan is gradually increasing since 1984--the mycobacteriosis research group of the japanese national chest hospitals]. | | 1988 | 3249525 |
| [changes in the biological properties of yersinia pestis during long-term intracellular multiplication]. | | 1988 | 3249571 |
| [the modifying action of normal human blood serum on vaccinal ev76 and virulent 363(1/1479) strains of yersinia pestis]. | | 1988 | 3249590 |
| [studies on stimulation of immunological responses resulting from intravesical bcg instillation in patients with bladder carcinomas]. | | 1988 | 3251104 |
| influenza-like syndrome in homosexual men: a prospective diagnostic study. | in the course of a prospective study of the prevalence and incidence of infection with the human immunodeficiency virus (hiv) and risk factors for the acquired immune deficiency syndrome among 961 homosexual men, 97 initially hiv antibody seronegative men reported a febrile period lasting at least three days. in 60 of these men serological evidence for an infection was found: influenza a or b virus (17 men), hiv (14), epstein-barr virus (seven), parainfluenza virus type i, 11 or ill (five), hepa ... | 1988 | 3256668 |
| glutathione transferase activities of cultured human lymphocytes. | we have detected glutathione transferase (gst) activity towards 1-chloro-2,4-dinitrobenzene (cdnb) and benzo[a]-pyrene 4,5-oxide (bpo) in freshly isolated peripheral lymphocytes, phytohaemagglutinin (pha)-stimulated lymphocytes, interleukin-2 (il-2)-dependent t-cells and lymphoblastoid b-cell lines. the detection of conjugating activity with bpo implies the expression of a neutral, 'mu-like' gst form by both resting and cultured lymphocytes. in a sample of 12 unrelated individuals, bpo conjugati ... | 1988 | 3257909 |
| inhibition of the proliferative response of human b lymphocytes to b cell growth factor by transforming growth factor-beta. | the effects of transforming growth factor-beta (tgf-beta) on the proliferative response of human b cells to the low molecular weight b cell growth factor (bcgf) have been investigated in this study. it was found that tgf-beta, at picomolar concentrations, strongly inhibited the bcgf-induced proliferation of anti-mu chain or staphylococcus aureus cowan i-activated human b cells and also of a bcgf-dependent cell line derived from a human lymphocytic nodular lymphoma. this inhibitory effect was det ... | 1988 | 3257917 |
| nonrandom trg gamma variable gene rearrangement in normal human t cells and t cell leukemias. | to estimate the extent of the trg gamma variable (v) gene repertoire used in human t cell ontogeny, we have analyzed the variety of v gamma gene rearrangements in a large series of t and non-t acute and chronic leukemias. a limited heterogeneity of rearranged fragments was observed: only 13 types of differently rearranged fragments, four of which occurred only once, were found among 80 rearranged chromosomes. furthermore, in the leukemic population as a whole, the frequency distribution of the m ... | 1988 | 3257920 |
| a monoclonal antibody for the specific diagnosis of plague. | a stable mouse-cell hybridoma was obtained that secretes an iga monoclonal antibody reactive with the fraction 1 (f1) envelope antigen of yersinia pestis. titres of the antibody typically ranged from 1:32 768 to 1:65 536 in mouse ascitic fluids.the monoclonal antibody formed a line of precipitation when run against f1 antigen in ouchterlony gel diffusion tests. in tests of 235 strains of y. pestis, lines of identity occurred between the precipitates formed with a solution of purified f1 antigen ... | 1988 | 3260145 |
| [a genetically defined model for testing teratogenicity in vivo]. | | 1988 | 3261883 |
| potent and comparable vasodilator actions of a- and b-calcitonin-gene-related peptides on the superficial subcutaneous vasculature of man. | 1. vasoactivity of human a- and b-calcitonin-gene-related peptides was studied in normal subjects, using reflectance plethysmography to measure relative changes in blood flow produced by superficial subcutaneous injections of the peptides. 2. injection of 10(-11) mol of either peptide caused an immediate 200% increase in local blood flow and prolonged hyperaemia lasting up to 3 h. the hyperaemic response to 10(-13) mol of each peptide was significantly (p less than 0.01) smaller and shorter in d ... | 1988 | 3262028 |
| receptor-mediated endocytosis and degradation of bovine growth hormone in rat liver. | receptor-mediated endocytosis of radiolabelled bovine growth hormone (125i-bgh) via somatogenic receptors in the liver was studied following in vivo intraportal injection. at different times after injection, subcellular membrane fractions involved in binding (plasma membranes), endocytosis (endocytic vesicles) and degradation (lysosomes) of peptide hormones were isolated by sucrose density gradient centrifugation. these fractions were evaluated for the time-course accumulation of radiolabelled b ... | 1988 | 3264255 |
| interleukin 1 as a tumor cytostatic mediator released from tumor ascites-treated macrophages. | peritoneal macrophages from dba/2 mice, elicited by injection of corynebacterium parvum (c.p.), were in vitro activated to eb tumor cytostasis by incubation with tumor-induced ascites that was harvested 7 days after intraperitoneal eb injection. the active cytostasis-mediating compound was found to be interleukin 1 (il 1). when tumor ascites was fractionated according to molecular weight size, the most active il 1-inducing fraction was found to comprise molecules of greater than 100,000 daltons. ... | 1988 | 3264264 |
| t-cell subsets in the cerebrospinal fluid and blood of patients with multiple sclerosis. | the absolute numbers and ratios of helper/inducer (t4) and cytotoxic/suppressor (t8) t-cells were determined in cerebrospinal fluid (csf) and blood of patients with multiple sclerosis (ms) and various other neurologic diseases (ond). in patients with ms, the t4:t8 ratio was higher in both blood and csf, and the increase was significantly greater in csf than in blood. these findings were due to an increased proportion of t4-lymphocytes in the csf and to a decreased proportion of t8-cells in blood ... | 1988 | 3264287 |
| suppressive and enhancing effect of t-2 toxin on murine lymphocyte activation and interleukin 2 production. | t-2 toxin, a fungal metabolite shown previously to exert potent immunosuppressive effects, was examined for its effects on activation and interleukin 2 (il 2) production by murine and rat splenocytes. splenocytes (1 x 10(6) cells/well) were incubated with 1 microgram concanavalin a (con a) for 48 h at which time cellular protein and dna synthesis by these cells were ascertained using radiolabeled precursors. il 2 synthesis was determined from the cell supernatant using the il 2-requiring cell li ... | 1988 | 3264300 |
| malaria infection in rats with stimulated splenic red pulp: the blood flow and protective effect in normal and transplanted splenic tissue. | after splenic autotransplantation both weight and blood flow of the regenerated splenic tissue are decreased. in addition, the protective function of the transplant is less compared to that of the normal spleen. in the present study, the red pulp of normal and transplanted splenic tissue was stimulated by injections of phenylhydrazine to increase the weight, the blood flow, and the protective function. after stimulation, the weight of the normal spleen increased to 900 +/- 70 mg (control 530 +/- ... | 1988 | 3265528 |
| optimal conditions for recovery of the human immunodeficiency virus from peripheral blood mononuclear cells. | optimal conditions for demonstrating the presence of infectious human immunodeficiency virus in peripheral blood mononuclear cells (pmcs) from seropositive individuals involved cocultivation of infected cells with phytohemagglutinin-stimulated pmcs from seronegative donors in the presence of 2 micrograms of polybrene per ml. the size of the culture vessel also influenced the results; smaller numbers of infected cells were detected under conditions of increased cell density. in addition, an incre ... | 1988 | 3266220 |
| circular intermediates with missing nucleotides in the conversion of supercoiled or nicked circular to linear duplex dna catalyzed by two species of bal 31 nuclease. | the extracellular nucleases from alteromonas espejiana bal 31 can catalyze the endonucleolytic and/or exonucleolytic hydrolysis of duplex dna in response to a variety of alterations, either covalent or noncovalent, in dna structure. the nuclease can exist as at least two kinetically and molecularly distinct protein species. the two species that have been studied, called the 'fast' (f) and 'slow' (s) nucleases, both readily convert negatively supercoiled dnas to linear duplex molecules and accomp ... | 1988 | 3275464 |
| plasmodium fragile: detection of a ring-infected erythrocyte surface antigen (resa). | a ring-infected erythrocyte surface antigen (resa) has been detected by modified immunofluorescence assay in erythrocytes infected with the simian malaria parasite, plasmodium fragile. this resa, of mr 95,000, shares many characteristics with the resa initially found in the human malaria parasite p. falciparum. both antigens are found in the membrane of erythrocytes infected with young asexual parasite stages, in merozoite-enriched preparations, and in parasite culture supernatant. since the res ... | 1988 | 3276549 |
| the effects of some synthetic prostanoids on the contractility of the human lower uterine segment in vitro. | we have investigated the ability of three synthetic prostanoids to directly influence uterine contractility by studying the effects in vitro. strips of lower uterine segment smooth muscle were obtained from women undergoing elective cesarean section at term. the ability of these strips to develop tension in the presence of cumulative additions of prostanoids or oxytocin was assessed. spontaneous contractions were inhibited by zk 96.480, a stable synthetic analog of prostaglandin i2, with a 50th ... | 1988 | 3277428 |
| [continuous nasal positive pressure respiration (ncpap) as a therapeutic possibility in the sleep apnea syndrome]. | sleep apnea syndrome (sas) is a rare disorder which is being diagnosed more often with increasing knowledge among physicians and patients. sas presents with daytime hypersomnolence, intellectual deterioration and personality changes, chiefly in obese men, and is caused by intermittent upper airway obstruction during sleep at the level of the mesopharynx. consecutive repetitive apneas of more than 10 seconds' duration are immediately abolished by pneumatic splinting with continuous positive press ... | 1988 | 3278374 |
| a comparison of culture, direct fluorescent antibody test, and a quantitative indirect immunoperoxidase assay for detection of chlamydia trachomatis in pregnant women. | an indirect immunoperoxidase assay that quantitates antichlamydial immunoglobulin g (igg) and iga antibodies in serum was compared with endocervical culture and direct fluorescent antibody (microtrak) for detection of chlamydia trachomatis in asymptomatic pregnant women. of the 64 women tested by the three methods, 22 (34%) had antichlamydial igg and iga in their serum. the culture was positive in nine patients (14%) and the microtrak was positive in eight (12.5%). all positive cultures were imm ... | 1988 | 3279354 |
| vitamin d binding protein (gc) subtypes by isoelectric focusing and immunoblotting. | the polymorphism of the human vitamin d binding protein (gc system) was investigated in a total of 149 sera from unrelated healthy egyptians residing in tanta city, gharbiya governorate, nile delta of egypt, using isoelectric focusing (ief) in thin-layer polyacrylamide gel followed by immunoblotting. the estimated gene frequencies were gc1s = 0.540, gc1f = 0.242 and gc2 = 0.218. | 1988 | 3280448 |
| biotyping of aerobic actinomycetes by modified killer system. | forty-four yeasts belonging to the genera pichia, candida, saccharomyces and kluyveromyces were tested for their potential killer effect on 13 aerobic actinomycetes (6 nocardia asteroides, 1 n. brasiliensis, 1 n. caviae and 5 actinomadura madurae). only a few yeast strains did not display any killer activity against the aerobic actinomycetes studied, thus confirming that the killer phenomenon is widespread among microorganisms. for epidemiological purposes, a killer system was developed. accordi ... | 1988 | 3281858 |
| [interrelation between the blood neutrophil damage test and the passive hemagglutination reaction in detecting antibodies]. | statistical analysis of the results of examinations of vaccinees against plague has revealed that the values of the neutrophil damage index (ndi) and antibody titers as determined in the passive hemagglutination (pha) test qualitatively (but not quantitatively) correlate. the statistical series for the pha test and ndi belong to different variations, i. e. they describe different functions of antibodies with respect to the same antigen. besides, the determination of ndi permits the detection of ... | 1988 | 3284255 |
| the plague pandemics and the discovery of the plague bacillus. | | 1988 | 3289591 |
| comparative biology of test species. | this paper assesses the capacity of animal models to predict human response to carcinogenic agents with consideration for the heterogeneity of humans. it is widely accepted that human susceptibility to toxic substances, including carcinogens, is highly variable. conventional rodent models are usually highly inbred and valued for their ability to display characteristic homogeneity. current practice assumes that the homogeneity of response to toxic agents, including carcinogens, in the rodent mode ... | 1988 | 3289908 |
| application of recombinant dna techniques in the development of viral vaccines. | the wide repertoire of methods developed for the construction and manipulation of recombinant dna molecules enable viral genomes to be cloned and their genes expressed, in whole or in part, in microbial or animal cells in culture. this provides a ready source of many viral components that are otherwise difficult to obtain and in some cases were previously unknown. although immediate applications for vaccine development have concentrated principally upon envelope proteins, the immunological impor ... | 1988 | 3291454 |
| sex difference in prolactin and growth hormone cells in mouse adenohypophysis: stereological, morphometric, and immunohistochemical studies by light and electron microscopy. | prl and gh cells in the adenohypophysis of adult mice were studied with immunohistochemistry and stereological morphometry by light and electron microscopy. we compared findings for males and females. in female mice, most prl-immunoreactive cells (43% of all parenchymal cells) and most gh-immunoreactive cells (33% of all parenchymal cells) were classical prl cells (type i prl cells) or gh cells (type i gh cells), respectively. besides these classical types of cells, we found prl- and gh-immunore ... | 1988 | 3293989 |
| deferoxamine inhibition of malaria is independent of host iron status. | the mechanism whereby deferoxamine (df) inhibits the growth of malaria parasites was studied in rats infected with plasmodium berghei. peak parasitemia was 32.6% (day 14) in untreated controls and 0.15% (day 7) in rats receiving 0.33 mg/g in 8 hourly df injections, subcutaneously. df inhibition of parasite growth was achieved without any reduction in transferrin saturation or hemoglobin synthesis and with only a partial (56%) depletion of hepatic iron stores. dietary iron depletion resulted in a ... | 1988 | 3294334 |
| evolution of beta-lactamase inhibitors. | beta-lactamase, the bacterial enzyme that can inactivate penicillins, cephalosporins and related antibiotics, can function outside the cell or in the periplasmic space. this resistance can be transferred between bacteria of the same or different species. most strains of staphylococcus aureus, staphylococcus epidermidis, klebsiella, enterobacter, pseudomonas aeruginosa and bacteroides species are beta-lactamase producers. clavulanic acid is a beta-lactamase inhibitor that works by blocking the en ... | 1988 | 3294401 |
| production losses and control of helminths in ruminants of tropical regions. | | 1987 | 3294655 |
| evaluation of the skin disinfecting activity and cumulative effect of chlorhexidine and triclosan handwash preparations on hands artificially contaminated with serratia marcescens. | the initial and cumulative efficacy of two antiseptic handwash preparations in eliminating serratia marcescens from hands was evaluated on volunteers. two antiseptics with persistent skin antibacterial activity, 4% chlorhexidine gluconate in detergent and 1.5% triclosan in natural soap, were studied in a new protocol designed according to food and drug administration guidelines. after a single handwash, both preparations exhibited a degerming action statistically superior to the mechanical elimi ... | 1987 | 3294700 |
| [field trial of an immunoenzyme method using beta-lactamase as the monoclonal antibody marker for the capsular antigen of the plague microbe]. | beta-lactamase (penicillinase) has been used as a marker of monoclonal antibodies in the enzyme immunoassay made with a view to the detection of y. pestis capsular antigen and antibodies to it. the trial of the enzyme immunoassay with the use of the beta-lactamase conjugate in laboratories and under field conditions has revealed the advantage of this assay over hemagglutination tests commonly used for this purpose. | 1987 | 3296577 |
| purification and analysis of rat hematopoietic stem cells by flow cytometry. | the monoclonal antibody ox7 recognizes an epitope expressed on the thy-1 glycoprotein, ox22 recognizes the high molecular weight form(s) on leukocyte common antigen, and w3/13 recognized determinants found on certain sialoglycoproteins. recently, the rat colony-forming unit spleen (cfu-s) was characterized as being ox7 upper 20% positive (ox7u20%), ox22 negative (ox22-), and w3/13 weakly positive (w3/13+). in the present study these observations have been extended to include the hematopoietic st ... | 1987 | 3297565 |
| epinephrine, glucose, and lactate infusion in exercising adrenodemedullated rats. | the purpose of this study was to determine the metabolic function of the marked increase in plasma epinephrine which occurs in fasted rats during treadmill exercise. fasted adrenodemedullated (adm) and sham-operated (sham) rats were run on a rodent treadmill (21 m/min, 15% grade) for 30 min or until exhaustion. adm rats were infused with saline, epinephrine, glucose, or lactate during the exercise bouts. adm saline-infused rats showed markedly reduced endurance, hypoglycemia, elevated plasma ins ... | 1987 | 3298192 |
| a simple method to eliminate the antigenicity of surface class i mhc molecules from the membrane of viable cells by acid treatment at ph 3. | we describe here a simple, reproducible method which specifically eliminates the antigenicity of surface class i major histocompatibility complex (mhc) molecules by acid treatment at ph 3 from the membrane of viable cells. when fresh mononuclear cells (mnc) or established cultured cell lines were treated at 4 degrees c for 2 min with citric acid buffer at ph 3 containing 1% bovine serum albumin, the antigenicity of class i mhc molecules, but not those of class ii mhc and the other non-mhc antige ... | 1987 | 3298442 |
| [structural and functional properties and the biological activity of yersinia pestis capsular antigen, murine toxin and endotoxin]. | | 1987 | 3300112 |
| [circulating immune complexes containing an insulin of bovine origin in a patient "allergic" to insulin]. | | 1986 | 3302476 |
| [the rodent. akodon arviculoides, wagner, 1842 (cricetidae)--importance in plague foci in brazil]. | the occurrence of the rodent akodon arviculoides wagner, 1842 in the plague focus of the "agreste" region of the state of pernambuco and a report on its ability for survival, reproduction and development in captivity, its susceptibility to yersinia pestis infection and the role of this rodent species in brazilian plague foci are reported. | 1986 | 3302594 |