| ecological studies on intestinal microbial flora of kenyan children with diarrhoea. | the intestinal microflora was analysed together with short-chain fatty acids (scfa) and bile acids in faeces from nine children with acute diarrhoeal disease in lari, kenya. enteric pathogens such as enteroinvasive e. coli, enteropathogenic e. coli, yersinia enterocolitica, rotavirus, giardia lamblia and entamoeba histolytica were isolated either singly or in combination from diarrhoeal faecal specimens. the most striking finding in these patients was a marked reduction of anaerobes. analysis of ... | 1990 | 2348500 |
| [ocular findings in infection-linked immune phenomena and secondary diseases (the so-called reiter's syndrome)]. | between 1981 and 1987 the authors saw 12 patients with reiter's syndrome. lesions included 6 cases of bilateral conjunctivitis in patients with chlamydial infection, 3 of unilateral serofibrinous iridocyclitis in patients with yersinia enterocolitica, 1 case of bilateral iridocyclitis in a patient with positive chlamydial complement-binding reaction, 1 case of bilateral follicular conjunctivitis following acute gonococcal urethritis, and one case of unilateral serofibrinous iridocyclitis, in a p ... | 1990 | 2348635 |
| effects of room-temperature exposure on bacterial growth in stored red cells. | experiments were performed to determine the effect of room-temperature (rt) exposure on the growth rates of selected bacteria by deliberate contamination of as-1 packed red cell (rbc) units contained in pl146 plastic bags. isolates of staphylococcus aureus, s. epidermidis, streptococcus faecalis, pseudomonas aeruginosa, citrobacter freundii, enterobacter agglomerans, and yersinia enterocolitica were inoculated into 3- to 5-day-old packed rbc units. the units were refrigerated at 4 to 6 degrees c ... | 1990 | 2349628 |
| epidemiologic investigations of yersinia enterocolitica and related species: sources, frequency, and serogroup distribution. | during an 11-year period (1978 to 1989), over 300 strains of yersinia spp. (excluding y. pestis and y. pseudotuberculosis) were recovered from a variety of gastrointestinal and extraintestinal sites in patients in california. over the 11-year period, y. enterocolitica serogroup o:3 predominated, although a shift in the relative frequency of this serogroup was observed during this interval, increasing dramatically during the years 1984 to 1989. of the remaining y. enterocolitica isolates, over 40 ... | 1990 | 2351734 |
| comparison of enrichment and plating media for isolation of yersinia. | yersinia enterocolitica (serotypes 0:3 or 0:8), yersinia frederiksenii, yersinia kristensenii, or yersinia intermedia along with 10(8) cells of each of three extraneous organisms (escherichia coli, enterobacter aerogenes, pseudomonas fragi), all commonly found on market poultry, were inoculated into five enrichment media followed by streaking onto 11 plating media to determine the most-efficacious combinations for future surveys or assessment studies. for yersinia enterocolitica (0:8), infrequen ... | 1990 | 2356183 |
| hickman catheter sepsis caused by yersinia enterocolitica. | | 1990 | 2356507 |
| yersinia enterocolitica infection in patients with iron overload. | | 1990 | 2358213 |
| case records of the massachusetts general hospital. weekly clinicopathological exercises. case 28-1990. a 72-year-old woman with abdominal pain and an ileocecal radiographic abnormality. | | 1990 | 2359420 |
| epidemiological, clinical and microbiological features of yersinia enterocolitica infections in a community during a four-year period. | epidemiological and clinical features of 17 cases (13 children) of yersiniosis treated in a hospital over a four-year period, as well as factors associated with virulence, antibiotic resistance and plasmids in yersinia enterocolitica strains from these patients were studied. the proportion of y. enterocolitica was 3.57% of all fecal cultures positive for enteric pathogens, with 13 sporadic episodes and one outbreak. in 11 cases the infection was unimicrobial. the clinical presentation was: gastr ... | 1990 | 2361543 |
| multiple measures of motor lateralization in human primates (homo sapiens). | laterality was assessed in 100 human (homo sapiens) subjects through the use of five measures of motoric behavior in three categories: locomotor, manual, postural. locomotor measures included leading limb for initiation of walking and whole-body turning. performance and questionnaire measures were used to assess lateral hand bias. postural bias was assessed as the weight distribution in quiet standing. population-level biases on the manual and leading limb measures were to the right; on posture ... | 1990 | 2364660 |
| relationship between dam's age at bull's birth and bull's genetic evaluation. | after accounting for year of birth of bulls, younger dams produced superior sons. bulls' pd, parent average, and pedigree index milk decreased with increasing age of dams at bull's birth. this appears to be due to decrease in maternal grandsire's pd milk and dam's cow index milk with increase in age of dam. younger dams have an advantage of potentially higher genetic merit due to genetic trend but have less information included in their evaluations. bull's latest parent average and pedigree inde ... | 1990 | 2365889 |
| [the efficacy of the extracorporeal connection of a porcine spleen in early and late septic complications in patients with burns]. | extracorporeal connection of a porcine spleen (eccps) was used in 36 patients ranging in age from 14 to 60 years with a iiiab-iv degree burn of from 30 to 80% of a body surface. the clinical signs of infective complications were the indication for eccps. the immunomodulating effect of eccps in burn septicotoxemia and normalizing effect on the morphofunctional state of neutrophil granulocytes without normalization of cellular immunity in toxemia was established. | 1990 | 2366448 |
| oral immunization of mice with a live recombinant yersinia enterocolitica o:9 strain that produces the cholera toxin b subunit. | the 70-kilobase pyv plasmid of yersinia enterocolitica encodes a set of proteins called yops that are produced during infection. to use y. enterocolitica as a live carrier to present the cholera toxin b (ct-b) subunit to the immune system, we constructed an operon fusion between ctxb and the yop51 gene. this operon fusion was either cloned on an rsf1010-derived plasmid or integrated into the pyv plasmid itself. in y. enterocolitica, both constructions directed the synthesis of free ct-b only und ... | 1990 | 2370100 |
| identification of a conjunctivitis-associated gene locus from the virulence plasmid of yersinia enterocolitica. | the virulence plasmid (pyv) of yersinia enterocolitica is necessary for production of conjunctivitis in guinea pigs and for mouse lethality. to identify the genes responsible for production of conjunctivitis in guinea pigs, we subcloned the bamhi and sali restriction fragments of the virulence plasmid of y. enterocolitica a2635 (serotype o:8) into derivatives of the broad-host-range plasmid prk290 and introduced the constructions into plasmid-negative y. enterocolitica strains. a mild, transient ... | 1990 | 2370102 |
| differentiation of serological responses to yersinia enterocolitica serotype o9 and brucella species by immunoblot or enzyme-linked immunosorbent assay using whole bacteria and yersinia outer membrane proteins. | serum samples from 134 patients showing by the microagglutination test serological cross-reactivity between yersinia enterocolitica serotype o9 and brucella spp. were analyzed by immunoblot and enzyme-linked immunosorbent assay techniques for the presence of antibodies directed against plasmid-encoded, yersinia-associated outer membrane proteins (omps). since these omps are exclusively expressed in pathogenic strains of yersinia spp., this characteristic was chosen for serological differentiatio ... | 1990 | 2380382 |
| [acute stenosing laryngotracheitis in a patient with yersinia and viral infections]. | | 1990 | 2385481 |
| isolation and structure of a novel charged member of the red-pigment-concentrating hormone-adipokinetic hormone family of peptides isolated from the corpora cardiaca of the blowfly phormia terraenovae (diptera). | a hypertrehalosaemic neuropeptide from the corpora cardiaca of the blowfly phormia terraenovae has been isolated by reversed-phase h.p.l.c., and its primary structure was determined by pulsed-liquid phase sequencing employing edman chemistry after enzymically deblocking the n-terminal pyroglutamate residue. the c-terminus was also blocked, as indicated by the lack of digestion when the peptide was incubated with carboxypeptidase a. the octapeptide has the sequence pglu-leu-thr-phe-ser-pro-asp-tr ... | 1990 | 2386478 |
| investigations into the route of uptake and pharmacokinetics of intraperitoneally-administered monoclonal antibodies: i. transdiaphragmatic blockade of the terminal lymphatics in the rat. | recent studies on the intraperitoneal administration of radiolabeled monoclonal antibodies indicate that the diaphragm and, in particular, the lymphatics associated with the diaphragm are more involved in the transport of such high-molecular-mass moieties than was earlier suspected. the current study examines the role of the diaphragm in the i.p. transport of an igg2a murine monoclonal antibody, 5g6.4, by observing the effect on the absorption of the antibody produced when the diaphragm has been ... | 1990 | 2386982 |
| [transfusion-induced yersinia infection]. | after a primary operation for renal artery stenosis a 48-year-old woman had to be re-operated twice for renewed arterial stenosis and retroperitoneal bleeding. the patient's respiratory and cardiovascular functions were stable two days after the second operation and renal function was recovering. but because of suspected renewed bleeding a transfusion with erythrocyte concentrate was begun. fifteen minutes later the patient started to shiver and this was followed by signs of cardiocirculatory de ... | 1990 | 2387216 |
| relapsing yersinia infection. | | 1990 | 2390549 |
| susceptibility of hla-b27 transgenic mice to yersinia enterocolitica infection. | the majority of patients with reactive arthritis have the major histocompatibility complex class i gene hla-b27. the development of arthritis in these patients often occurs following infection with one of several enteric bacteria, including yersinia enterocolitica. in this study, transgenic mice expressing hla-b27 and their negative full sibs were infected intravenously with yersinia enterocolitica 0:8 wa in an attempt to develop an experimental model of reactive arthritis. to date, no reactive ... | 1990 | 2391253 |
| tertiary structure of oxidized flavodoxin from an eukaryotic red alga chondrus crispus at 2.35-a resolution. localization of charged residues and implication for interaction with electron transfer partners. | the crystal structure of the oxidized form of a flavodoxin from an eukaryotic red alga, chondrus crispus, has been determined by multiple isomorphous replacement and anomalous scattering methods. a model of the 173 residues and flavin mononucleotide (fmn) has been refined by a restrained least squares method to a crystallographic r-factor of 22.6% using 6236 reflections between 6.0 and 2.35 a with f greater than 3 sigma f. this molecule has a sheet consisting of five parallel beta-strands with t ... | 1990 | 2394748 |
| onset of the hormone-sensitive perinatal period for sexual differentiation of the sexually dimorphic nucleus of the preoptic area in female rats. | the volume of the sexually dimorphic nucleus of the preoptic area (sdn-poa) of the rat brain is severalfold larger in males than in females. the volume of the sdn-poa can be influenced significantly by the hormonal milieu during the perinatal "critical period" of sexual differentiation of the brain. the purpose of the present study was to determine the onset of this period of sexual differentiation of the sdn-poa. pregnant rats received no treatment or were injected subcutaneously with oil on da ... | 1990 | 2394991 |
| [the anginal form of yersiniosis]. | the anginal form of yersiniosis is described in 16 patients (1.3% of all patients with this kind of infection). clinico-laboratory investigations revealed signs of a generalized process with bacteremia and toxemia. the syndrome complex of infectious mononucleosis is often observed in this form of yersiniosis. two patients showed a diphtheria-like form of severe angina. in 31% of patients recurrence developed but usually without angina. the outcomes are favourable. | 1990 | 2396379 |
| factors affecting production and activity of phospholipase c by yersinia enterocolitica. | the production of phospholipase c by yersinia enterocolitica strain sg was optimum at 37 degrees c at ph 6.5. no enzyme activity could be detected when the organism was grown at extreme ph values (ph greater than 8.5 or less than 5.0). the enzyme production was maximum when the organism was grown under static conditions in tsb medium. all solvents and salts inhibited the enzyme activity, whereas loss of activity was 95% in presence of methanol (20%) and 99% in presence of sodium azide (0.2 mol/l ... | 1990 | 2398028 |
| does a retrovirus explain fatigue syndrome puzzle? | | 1990 | 2399461 |
| the prevalence of yersinia enterocolitica 0:3 in finnish pigs and pork. | one hundred and forty seven samples of pig faeces collected from 14 herds located in different parts of finland were examined for yersinia enterocolitica biotype 4 serotype 0:3. twenty six (17.7%) animals and 5 herds (35.7%) were positive. the tonsils of 350 animals from 35 herds with high condemnation figures at meat inspection and the tonsils of 131 animals from 13 herds with low condemnation figures collected from 2 abattoirs in southwest finland were also examined. the prevalence raes of y.e ... | 1990 | 2399870 |
| yersinia enterocolitica coxitis after hip replacement. a case report. | we report a case of hematogenous yersinia enterocolitica coxitis 10 years after hip replacement. despite extraction of the prosthesis and antibiotic treatment, the infection relapsed. infections in replaced joints with yersinia enterocolitica call for long-standing treatment with specific antibiotics. | 1990 | 2402993 |
| disseminated yersinia enterocolitica. case report and review of the literature. | yersinia enterocolitica is an uncommon cause of infectious diarrhea in the united states. rarely, it is a cause of extraintestinal disease. yet an association exists between y. enterocolitica infection and axial arthropathy. we present a patient with long-standing arthritis, consistent with incomplete reiter's syndrome, who developed y. enterocolitica colitis with hepatic and pulmonic infection. | 1990 | 2406333 |
| in vitro antimicrobial activity of bismuth subsalicylate and other bismuth salts. | this report demonstrates that bismuth subsalicylate (bss) effectively inhibits growth of a number of bacterial strains known to cause diarrhea, including escherichia coli, salmonella, shigella, and campylobacter. other bismuth salts and sodium salicylate, a hydrolysis product of bss in the gut, also were examined and were shown to have various degrees of activity. growth of the organisms was monitored in vitro by inoculating culture fluid that contained one of the compounds to be tested and dete ... | 1990 | 2406851 |
| a heat-modifiable outer membrane protein carries an antigen specific for the species yersinia enterocolitica and yersinia pseudotuberculosis. | the outer membranes of gram-negative bacteria are considered to be of importance in host-bacteria interaction, in protective immunity, and occasionally in subclassification within a species. in this study, the outer membranes of several strains of yersinia enterocolitica and y. pseudotuberculosis were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). it was found that the appearance of the major proteins depended on the temperature at which they were solubilized i ... | 1985 | 2409152 |
| structural studies on o-specific polysaccharides of lipopolysaccharides from yersinia enterocolitica serovars o:1,2a,3, o:2a,2b,3 and o:3. | lipopolysaccharides from yersinia enterocolitica serovars o:1,2a,3, o:2a,2b,3 and o:3 have been isolated and characterized. 6-deoxy-l-altrose residues were shown to be the main constituents of lipopolysaccharides isolated in addition to residues of l-rhamnose, d-glucose, d-galactose, 2-acetamido-2-deoxy-d-glucose, 2-acetamido-2-deoxy-d-galactose, d-glycero-d-manno-heptose and l-glycero-d-manno-heptose, 3-deoxy-d-manno-octulosonic acid being minor components of sugar chains. mild hydrolysis of li ... | 1985 | 2410273 |
| projection systems and terminal localization of dorsal column afferents: an autoradiographic and horseradish peroxidase study in the rat. | projection systems from the gracile nucleus and the cuneate nuclear complex to their terminal sites in the mesencephalon, diencephalon, and cerebellum were examined by means of anterograde autoradiography and retrograde horseradish peroxidase methods. three projection systems emerge from the dorsal column nuclei, decussate via internal arcuate fibers, and form the contralateral medial lemniscus (ml). at the obex, some fibers split off the ml and course dorsolaterally, forming an ascending latera ... | 1985 | 2413085 |
| characterization of an unique rna initiated immediately upstream from human alpha 1 globin gene in vivo and in vitro: polymerase ii-dependence, tissue specificity, and subcellular location. | we have identified an abundant transcript initiated upstream from the canonical cap site of human alpha 1 globin gene in bone marrow cells and in cos-7 cells transfected with an alpha 1 globin gene-containing plasmid. similar to the major alpha 1 globin transcript, this upstream rna is present almost exclusively in the cytoplasm of the transfected cos-7 cells. it is also synthesized efficiently in vitro by rna polymerase ii in the nuclear extracts prepared from a hela cell line and an erythroleu ... | 1985 | 2413422 |
| natural killer cytotoxic factor induction by k562 cells in patients with advanced cancer: correlation with production of interferon. | advanced cancer patients were studied for their ability to produce natural killer cytotoxic factor (nkcf). of 23 patients with advanced epithelial cancers, 8 showed deficient natural killer (nk) activity, as measured in a standard 51cr release assay. lymphocytes from these patients did not generate nkcf (nonproducers) in the presence of k562 cells. in addition, 7 other patients whose nk activity was in the normal range did not generate nkcf. thus the deficiency in nkcf production was only partia ... | 1985 | 2415742 |
| [occurrence and level of antibodies against yersinia enterocolitica o antigens detected in select human groups by the passive hemagglutination test]. | | 1985 | 2421117 |
| anti-hla-b27 antibodies in sera from patients with gram-negative bacterial infections. | several forms of seronegative polyarthritis are strongly associated with hla-b27, and a number of microorganisms have been implicated in the etiology of these diseases. to explain the association between hla-b27 and arthritis initiated by infection with these organisms, it has been proposed that there is immunologic cross-reactivity between the b27 molecule and 1 or more microbial antigens, and that this cross-reactivity leads to tolerance to such infection and/or to the production of anti-hla-b ... | 1986 | 2421739 |
| structural studies on o-specific polysaccharides of lipopolysaccharides from yersinia enterocolitica serovars o:5 and o:5,27. | lipopolysaccharides of yersinia enterocolitica serovars o:5 and o:5,27 were shown to have a similar sugar composition, consisting of l-rhamnose, d-glucose, d-galactose, d- and l-glycero-d-manno-heptose, 2-acetamido-2-deoxy-d-glucose, 2-acetamido-2-deoxy-d-galactose, 3-deoxy-d-manno-octulosonate and d-threo-pent-2-ulose (d-xylulose). partial hydrolysis of lipopolysaccharides with acetic acid produced rhamnans with the following repeating unit: ----3)-l-rha rho(alpha 1----3)-l-rha rho(alpha 1----3 ... | 1986 | 2422030 |
| serological cross-reactions between brucella abortus and yersinia enterocolitica 0:9. | yersinia enterocolitica (ye) 0:9 is an organism of great significance in veterinary medicine largely as a result of its cross-reaction with brucella abortus (ba). boty ye 0:9 and ba possess somatic antigens in common; as a result of which animals exposed to ye 0:9 have an immune response which is distinguishable only with difficulty from that induced by ba. cattle were exposed to ye 0:9 by the oral or intramammary routes. oral exposure failed to generate significant serologic response. in contra ... | 1985 | 2422134 |
| predicted conformations for the immunodominant region of the circumsporozoite protein of the human malaria parasite plasmodium falciparum. | the circumsporozoite protein of the human malaria parasite plasmodium falciparum contains multiple tandem repeats of the amino acid sequence asn-ala-asn-pro. the repeated sequence encompasses the immunodominant region of the protein, and antibodies raised against it are potent inhibitors of invasion and development of sporozoites in cultured hepatocytes. using a modified build-up procedure, we have explored a large number of possible helical and near-helical conformations of a terminally blocked ... | 1986 | 2426702 |
| studies on the serology of flagellar antigens of yersinia enterocolitica and related yersinia species. | a total of 1242 strains of y. enterocolitica, 104 strains of y. frederiksenii, 95 strains of y. kristensenii and 85 strains of y. intermedia were serotyped with antisera against 56 o antigens and 19 h antigens according to the extended antigenic scheme of wauters, and with additional antisera against 4 somatic and 19 flagellar antigens not previously described. h antigens of y. frederiksenii, y. kristensenii, and y. intermedia turned out to be rather homogeneous without distinct subfactors. in t ... | 1986 | 2426873 |
| identification of yersinia enterocolitica o15 lipopolysaccharide as a rough antigen. | lipopolysaccharide (lps) of a reference strain of yersinia enterocolitica serovar o15, ye614, was examined for its chemical structure and antigenicity. the o15 lps was partially hydrolyzed with diluted acetic acid and fractionated on a sephadex g-50 column. only one peak was detected at fractions where the r-core oligosaccharide of a y. enterocolitica rough strain was eluted. the chemical compositions of this oligosaccharide and the o15 lps were similar to those of the r-core oligosaccharide and ... | 1986 | 2427540 |
| monoclonal antibodies against escherichia coli heat-stable toxin (sta) and their use in a diagnostic st ganglioside gm1-enzyme-linked immunosorbent assay. | seven monoclonal antibodies (mabs) against heat-stable enterotoxin (st) from a human escherichia coli isolate were prepared and evaluated for their usefulness in an st immunodetection assay, the st ganglioside gm1-enzyme-linked immunosorbent assay (elisa). this assay is based on the ability of sta, as present in, for example, culture filtrates from st-producing e. coli, to inhibit specific anti-st antibody from binding to solid-phase-bound st ganglioside (gm1-bound st-cholera b subunit). four of ... | 1986 | 2429984 |
| aminergic neurotransmitter and water content changes in rats after transient forebrain ischemia. | we have studied changes of cerebral monoamine metabolism and water content, during recirculation following global transient ischemia (20 min) using the four-vessel occlusion model in rats. levels of monoamines and their metabolites were determined in cortex, striatum, hippocampus, and hypothalamus. water content was evaluated by weight and by the analysis of t1 and t2 relaxation times in 1h-nuclear magnetic resonance. norepinephine levels decreased; 3,4-dihydroxyphenylethylamine, 3,4-dihydroxyph ... | 1986 | 2430056 |
| aminergic neurotransmitter and water content changes in rats after transient forebrain ischemia. | we have studied changes of cerebral monoamine metabolism and water content, during recirculation following global transient ischemia (20 min) using the four-vessel occlusion model in rats. levels of monoamines and their metabolites were determined in cortex, striatum, hippocampus, and hypothalamus. water content was evaluated by weight and by the analysis of t1 and t2 relaxation times in 1h-nuclear magnetic resonance. norepinephine levels decreased; 3,4-dihydroxyphenylethylamine, 3,4-dihydroxyph ... | 1986 | 2430056 |
| [effects of enzymatic deglycosylation of human goiter thyroglobulin on its immunochemical properties]. | thyroglobulin (tg), isolated from soluble iodoproteins by ammonium sulphate fractionation, was enzymatically deglycosylated in vitro and analyzed by polyacrylamide gel electrophoresis, double immunodiffusion and non-commercial ria. carbohydrate and iodine content was chemically determined. by paage deglycosylated tg (dtg) showed the appearance of a major band in the 12s region and three slower migrating bands corresponding to higher aggregates than 19s tg. in immunodiffusion by testing native an ... | 1986 | 2430684 |
| specificity of oligoclonal igg bands against myelin proteins in chronic relapsing eae in guinea pigs. | we showed previously by using imprint electroimmunofixation that the oligoclonal igg in sera and csf from chronic relapsing eae in guinea pigs were specific to spinal cord and mycobacterium tuberculosis. we now show that most oligoclonal igg bands are directed predominantly against isolated myelin basic protein (mbp). activity to the latter could be removed from sera or csf by absorption with mbp but not with histone or lysozyme. the oligoclonal igg reacted weakly with isolated proteolipid apopr ... | 1987 | 2433329 |
| effects of metoprolol, alone and in combination with lidocaine, on ventricular fibrillation threshold: comparison with atenolol, propranolol, and pindolol. | metoprolol and other beta-adrenergic blocking drugs are known to exert cardioprotective effects that include significant reduction in occurrence of ventricular fibrillation (vf) following myocardial ischemia and infarction. to help determine the mechanism of these cardioprotective effects, this study evaluated the effect of equipotent beta-blocking doses of metoprolol and three other beta-blockers with differing ancillary properties on ventricular fibrillation threshold (vft) in the normal canin ... | 1987 | 2439844 |
| immunoblotting analysis of human igm, igg and iga response to chromosomally coded antigens of yersinia enterocolitica 0:3. | human antibody response after yersinia enterocolitica infection was studied by immunoblotting sequentially collected sera against a whole-cell homogenate of y. enterocolitica serotype 0:3, grown under conditions restrictive for the plasmid. the antibodies observed were directed against a multitude of chromosomally coded antigens, and a considerable individual heterogeneity was found in the reactions of individual sera. the early (0-2 months) and late (greater than or equal to 11 months) response ... | 1987 | 2440235 |
| factors contributing to the reduced invasiveness of chlorine-injured yersinia enterocolitica. | the invasion of epithelial cells in vitro and in vivo by chlorine-injured yersinia enterocolitica was assessed by direct microscopic observations. these experiments showed that injury by chlorine inhibited invasiveness of virulent y. enterocolitica. two requirements appeared to be necessary for invasiveness: the organism must be viable and metabolically active, and the organism must have certain surface components to initiate engulfment. inhibition of rna synthesis by rifampin and protein synthe ... | 1987 | 2440382 |
| [enhancement of the therapeutic efficacy of blood substitutes in burn shock]. | | 1987 | 2440759 |
| a new set of monoclonal antibodies to human mhc class ii alpha chains demonstrates that most alpha epitopes are inaccessible on the living cell surface. | when mice were immunized with a mixture of human mhc class ii alpha and beta glycoprotein chains, the predominant antibody response was anti-alpha, and from a subsequent fusion experiment over 60 hybridomas showing anti-alpha activity were generated, compared with 11 anti-beta secretors. these findings contrast with the relative paucity of anti-alpha monoclonals described previously. use of a miniaturized western blot screening protocol was a critical factor in the present study since the anti-a ... | 1987 | 2440796 |
| a new set of monoclonal antibodies to human mhc class ii alpha chains demonstrates that most alpha epitopes are inaccessible on the living cell surface. | when mice were immunized with a mixture of human mhc class ii alpha and beta glycoprotein chains, the predominant antibody response was anti-alpha, and from a subsequent fusion experiment over 60 hybridomas showing anti-alpha activity were generated, compared with 11 anti-beta secretors. these findings contrast with the relative paucity of anti-alpha monoclonals described previously. use of a miniaturized western blot screening protocol was a critical factor in the present study since the anti-a ... | 1987 | 2440796 |
| discrimination of hepatocyte-stimulating activity from human recombinant tumor necrosis factor alpha. | the involvement of tumor necrosis factor alpha (tnf alpha) in the regulation of acute-phase protein synthesis is currently under discussion. in this study the effect of human recombinant tnf alpha on the regulation of the 4 acute-phase proteins alpha 2-macroglobulin, albumin, alpha 1-proteinase inhibitor and alpha 1-acute-phase globulin was investigated in rat hepatocyte primary cultures. no changes in synthesis of any of the 4 proteins were observed. however, an acute-phase response similar to ... | 1987 | 2441999 |
| immunoblot analysis of human igm, igg and iga responses to plasmid-encoded antigens of yersinia enterocolitica serovar o3. | human igm, igg and iga responses after infection with yersinia enterocolitica serovar o3 were studied by immunoblotting sera against whole-cell homogenates of a plasmid-containing strain of y. enterocolitica o3 and a plasmid-free strain derived from it; each strain was grown in conditions expressive for the plasmid. the antibodies observed were directed against several plasmid-encoded polypeptides. the response against different bacterial components decreased uniformly with time and the persisti ... | 1987 | 2443704 |
| analysis of the effects of inhaled diesel exhaust on the alveolar intravascular and interstitial cellular components of rodent lungs. | transmission electron microscopy (tem) was used to determine the effect of diesel engine exhaust (dee) on the intravascular and interstitial cellular population of the lungs of exposed rats and guinea pigs. animals with matched controls were subjected to environments of either 250, 750, 1500 or 6000 micrograms/m3 for either 2 weeks, 6 weeks, 10 weeks or 18 months. these animals were sacrificed immediately following the exposure periods and their lungs perfused with fixative. following dissection ... | 1987 | 2443965 |
| diagnostic importance of h-antigens in yersinia enterocolitica and other yersinia species. | h-antigens of 1,622 strains of y. enterocolitica, y. frederiksenii, y. kristensenii, and y. intermedia were examined according to the antigenic scheme of wauters in order to evaluate the importance of their antigens for the species diagnosis and epidemiological purposes. yersinia are monophasic bacteria with species-specific h-antigens. flagellar antigens of y. frederiksenii, y. kristensenii, and y. intermedia turned out to be rather homogeneous without distinct subfactors. the scope of identifi ... | 1987 | 2444390 |
| [a new branched-chain monosaccharide from the yersinia enterocolitica serotype o:4.32 lipopolysaccharide]. | a component of the yersinia enterocolitica o:4,32 lipopolysaccharide has been shown to be a second representative of the new class of monosaccharides and possess the structure of 3,6-dideoxy-4c-(1-hydroxyethyl)-d-xylo-hexose (yersiniose). | 1987 | 2445358 |
| influence of neuropeptides on airway smooth muscle. | the first bronchoactive (and vasoactive) peptide to be discovered in the lung was isolated and characterized in 1970 from an embryologically related organ, the small intestine. since then, more than 20 additional peptides have been described in lung tissue and their biologic activities investigated. many of these are neuropeptides acting as neurotransmitters or neuromodulators to influence airway, pulmonary vascular, and other functions. more neuropeptides are known to exist in the brain and per ... | 1987 | 2446543 |
| definition of myasthenogenic sites of the human acetylcholine receptor using synthetic peptides. | experimental autoimmune myasthenia gravis (eamg) and antibodies that modulate achrs from cultured human muscle are induced by a disulfide-looped peptide comprising the human acetylcholine receptor (achr) alpha-subunit residues 125-147 (h alpha 125-147). to delineate the essential antigenic requirements for induction of eamg by this peptide, a series of peptides was synthesized: (a) a nonlooped analog (cys 128 replaced by ser) stimulated modulating autoantibodies, induced eamg, and bound antibodi ... | 1987 | 2446556 |
| immunosuppressive effects of isoprinosine in man: a comparison to chlorambucil effects in multiple sclerosis. | immunological and clinical functions were studied over a 2 year period in conjunction with a placebo controlled trial of isoprinosine and chlorambucil in 21 patients with exacerbating remitting multiple sclerosis. laboratory and clinical evaluations were performed at 3 month intervals and during relapses. in placebo-treated patients, the decrease in circulating t8+ cells was maximum during relapses, t lymphocyte function was impaired, and five of the six patients experienced clinical worsening. ... | 1987 | 2446758 |
| immunological and biochemical characterization of an epitope of the transferrin receptor involved in the production of interferon-gamma and b-cell growth factor. | we have investigated the effect of a newly developed monoclonal antibody, moab nda9, on human lymphocyte function. this moab inhibits the capacity of peripheral blood lymphocytes to display blastogenic responses and to produce immunoglobulins when stimulated in vitro with pwm or with soluble antigens. the inhibitory effect seems to result from the decreased ability of t lymphocytes to produce b cell growth factors (bcgf) in the presence of moab nda9. this antibody also blocks the capacity of pol ... | 1987 | 2446777 |
| rapid staining of campylobacter pyloridis. | | 1987 | 2447004 |
| isolation of simian immunodeficiency virus from african green monkeys and seroepidemiologic survey of the virus in various non-human primates. | sixteen isolates of simian retrovirus closely related to human immunodeficiency virus (hiv) were obtained from healthy african green monkeys (agm) (cercopithecus aethiops). the first isolate was obtained from a monkey seropositive for hiv, and the others were isolated from monkeys harboring antibodies to the first isolate. these simian retroviruses were referred to as simian immunodeficiency virus from agm, siv[agm], due to their cross-reactivities with hiv structural proteins. these siv[agm] is ... | 1988 | 2447023 |
| chinese hamster ovary cell lysosomes rapidly exchange contents. | we have used cell fusion to address the question of whether macromolecules are rapidly exchanged between lysosomes. donor cell lysosomes were labeled by the long-term internalization of the fluid-phase pinocytic markers, invertase (sucrase), lucifer yellow, fitc-conjugated dextran, or texas red-conjugated dextran. recipient cells contained lysosomes swollen by long-term internalization of dilute sucrose or marked by an overnight fitc-dextran uptake. cells were incubated for 1 or 2 h in marker-fr ... | 1987 | 2447096 |
| changes of chromatin organization induced by phospholipids. | isolated nuclei represent a suitable model for studying the influence of exogenous phospholipids, normally found as minor chromatin components, on the nuclear structure, which, in turn, could be related to the observed modifications of dna and rna synthesis. the morphological modifications induced on chromatin rnp granules and nuclear matrix have been analyzed both with conventional thin sectioning and with an original method based on image analysis of freeze-fractured and replicated nuclear sam ... | 1987 | 2447872 |
| protein hc-iga complexes carry antibody activities. | polyclonal protein hc-iga complexes (hc-iga) were isolated from two different serum pools. their hydrodynamic volumes were found to be slightly greater than that of monomeric iga but less than that of dimeric iga. sodium dodecyl sulphate (sds)-polyacrylamide gel electrophoresis of reduced and carboxymethylated complexes followed by immunoblotting showed that the complexes contained normal light and heavy ig chains, and one polypeptide chain with mr = 90,000, which carried both iga alpha chain an ... | 1988 | 2448867 |
| monoclonal antibodies and characterization of epitopes of smooth brucella lipopolysaccharides. | four mouse monoclonal antibodies generated against brucella melitensis 16m, and three generated against b. suis 1330 were analysed. an elisa (enzyme-linked-immunosorbent assay) with whole cells as antigens was used to determine cross-reactivities with other gram-negative bacteria. two antibodies showed cross-reactivity with smooth brucella strains only. five antibodies also reacted with yersinia enterocolitica o:9, but not with other bacteria. two of these antibodies had significantly higher tit ... | 1987 | 2449902 |
| antigenic homologies between plasmid encoded proteins from enteropathogenic yersinia and thyroid autoantigen. | | 1987 | 2450826 |
| [a method of detecting inclusions of chlamydia trachomatis in pathological material]. | | 1988 | 2453733 |
| serological and biochemical characteristics of 416 yersinia strains from well water and drinking water plants in the federal republic of germany: lack of evidence that these strains are of public health importance. | a total of 416 yersinia strains from well water and drinking water plants in the federal republic of germany was analysed at the national reference centre for salmonella, hamburg, in the period 1982 to 1987. of these, 341 (82%) strains were y. enterocolitica, 46 (11%) strains were y. intermedia, 24 (5.8%) strains were y. frederiksenii, and 5 strains (1.2%) y. kristensenii. the serogroups o:3, o:9, and o:5,27 which in central europe are associated with human disease, were not isolated. seventy-tw ... | 1988 | 2453981 |
| use of monoclonal antibodies to identify the distribution of a and m epitopes on smooth brucella species. | the smooth types of brucella species express two lipopolysaccharides (lpss) (a and m) which are antigenically distinct. their existence as cross-reactive antigenic complexes makes definitive serology difficult. murine hybridomas were produced and selected for their ability to produce monoclonal antibodies to the specific a- or m-lps epitopes. the specificity of the monoclonal antibodies was determined by microplate enzyme-linked immunoassay, binding inhibition assay, microplate agglutination, an ... | 1988 | 2457599 |
| [the structure of o-specific polysaccharide from yersinia enterocolitica serotype o:6.31 lipopolysaccharide]. | the serologically active o-specific polysaccharide has been isolated from the lipopolysaccharide of yersinia enterocolitica, serovar o: 6.31. using methylation, partial acid hydrolysis and 13c nmr spectroscopy, the main structural moiety of the o-specific polysaccharide is shown to be the following disaccharide repeating unit: (formula: see text). | 1988 | 2458735 |
| mechanism of action of galanin on myenteric neurons. | 1. conventional intracellular recording methods were used to investigate the mechanism of action of galanin on electrical behavior of ah/type 2 myenteric neurons in the guinea pig small intestine. 2. the overall action of galanin was inhibitory and consisted of membrane hyperpolarization, decreased input resistance, and suppression of excitability. 3. the action of galanin was on the somatic membrane. there were no effects on spike initiation or propagation velocity in the processes. 4. the reve ... | 1988 | 2459321 |
| detection of cross-reacting epitopes on plasmid-encoded outer membrane proteins of enteropathogenic yersinia by monoclonal antibodies. | plasmid-positive yersinia bacteria of different species and different serotypes were analysed with respect to the immunological relationship of two of their plasmid-encoded outer membrane proteins (omps) by the immunoblot technique using three monoclonal antibodies (mab), which were induced against omps of yersinia enterocolitica serotype o:9 bacteria. evidence is presented that these omps display discrete epitopes, which are common to all plasmid-positive yersinia strains tested, with only one ... | 1989 | 2462666 |
| virulence associated plasmid and r-plasmid curing in yersinia enterocolitica by some tricyclic compounds. | the virulence plasmid which confers calcium-dependent growth in yersinia enterocolitica and the r-plasmid of a y. enterocolitica strain were eliminated by several, tricyclic psychopharmacons, e.g. promethazine, imipramine, and (+) and (-)-butaclamols. | 1988 | 2465656 |
| enzyme-linked immunosorbent assay for differentiation of the antibody response of cattle naturally infected with brucella abortus or vaccinated with strain 19. | purified o chain of brucella abortus was passively attached to polystyrene to differentiate antibody responses of cattle vaccinated with b abortus strain 19 from those of naturally infected cattle. in the indirect assay, using o polysaccharide as antigen, a single serum dilution was used and mouse monoclonal antibody to bovine l chain conjugated with horseradish peroxidase was the detection reagent. measurable antibody was not found in sera of vaccinated cattle, except for 3 sera from cattle tha ... | 1989 | 2465711 |
| immunity in human schistosomiasis mansoni: cross-reactive igm and igg2 anti-carbohydrate antibodies block the expression of immunity. | we have previously reported that the slow development of immunity to reinfection after treatment of schistosoma mansoni infections is partly attributable to the continued presence of 'blocking' antibodies in young, susceptible children. a further analysis of this phenomenon supports the hypothesis that such blocking antibodies can be of the igg2 as well as the igm isotype, and that they react with carbohydrate epitopes expressed both on egg polysaccharides and on schistosomulum surface antigens, ... | 1988 | 2465785 |
| the gene coding for the 190,000-dalton iron-regulated protein of yersinia species is present only in the highly pathogenic strains. | a genomic library containing dna fragments of 0.5 to 2 kilobase pairs in length from yersinia enterocolitica serovar o:8 was constructed in a bacteriophage lambda gt11 expression vector. mouse antibodies specific for the iron-regulated high-molecular-weight proteins (hmwps) were used to screen the library. two positive clones of 1 and 0.5 kilobase pairs, designated a13 and d7, respectively, were detected and isolated. they coded for beta-galactosidase fusion proteins of 151,000 and 138,000 dalto ... | 1989 | 2466794 |
| response of synovial fluid t cell clones to yersinia enterocolitica antigens in patients with reactive yersinia arthritis. | from synovial fluids of two patients with reactive arthritis following yersinia enterocolitica infection, t lymphocyte clones were obtained that showed proliferative responses to y. enterocolitica. the responses required autologous t-cell-depleted peripheral blood mononuclear cells as antigen presenting cells. three clones were studied in detail; two of them showed a marked and specific response to yersinia antigens alone, the other one recognized both yersinia and salmonella typhimurium antigen ... | 1989 | 2467775 |
| [immunochemical study of the lipopolysaccharides of yersinia enterocolitica serovars o5 and o5.27]. | y. enterocolitica lipopolysaccharides (lps), serovars o5 and o5.27, have similar antigenic specificity. the lps of serovar o5.27 has been shown to contain no factor o27. residual alpha-l-rhamnose, glycosylated by d-xylulose, has been found to play an important role in the formation of factor o5, common for both serovars. | 1988 | 2469272 |
| [the use of bismuth-sulfite-agar as a solid differential medium for isolating bacteria of the species y. enterocolitica and y. frederiksenii]. | the potentialities of bismuth-sulfite-agar (bsa) medium as a differentiating medium for the isolation of y. enterocolitica and the related species have been under study. y. enterocolitica colonies in bsa had a characteristic appearance and consistency. 7 y. enterocolitica and 2 y. frederiksenii strains have been isolated in 4.7% of various environmental specimens studied with the use of the bsa medium. bsa appears to be a promising medium for the isolation of yersinia from the clinical material ... | 1989 | 2470976 |
| [a comparative evaluation of the growth of yersinia enterocolitica on elective media]. | the aim of the work has been to find the optimal selective medium for the isolation of different serological and biochemical variants of y. enterocolitica. endo's, ploskirev's, lewin's, and bismuth sulfite media have been under study. hottinger's agar, ph 7.2, has been used for the inoculation dose control. forty y. enterocolitica strain isolated from humans and animals have been employed in the study. thirty-eight strains have been referred to 8 most prevalent serological variants and to 5 bioc ... | 1989 | 2470980 |
| expression in yersinia enterocolitica of k99 antigen gene of escherichia coli. | the expression of the determinant for the k99 antigen of escherichia coli in yersinia enterocolitica avirulent transconjugants d29(pfs239) and l15(pfs239) was confirmed by means of sds-polyacrylamide gel electrophoresis, double immunodiffusion and western blotting. the conditions for the expression of k99 antigen genes in y. enterocolitica and in e. coli were compared. the k99 antigen protein expressed in y. enterocolitica in the form of pili on the surface of the cells was discussed. this artic ... | 1988 | 2472004 |
| [structure of the o-specific polysaccharide of the yersinia enterocolitica serovar o:4.32 lipopolysaccharide. serologic relations of lipopolysaccharides of y. enterocolitica o:4.32 and y. intermedia o:4.33]. | o-specific polysaccharide has been isolated on mild acid hydrolysis of the lipopolysaccharide from yersinia enterocolitica o: 4.32 (strain 96) and shown to consist of yersiniose b (3,6-dideoxy-4-c-(1-hydroxyethyl)-d-xylo-hexose, yerb) acetylated at c1' and 2-acetamido-2-deoxy-d-galactose residues in a molar ratio 1:2. acid hydrolysis, methylation and 13c nmr studies indicated the polysaccharide to be composed of trisaccharide repeating units of the following structure: (sequence; see text) the d ... | 1989 | 2472796 |
| definition of brucella a and m epitopes by monoclonal typing reagents and synthetic oligosaccharides. | the paradigm that brucella a and m epitopes are simultaneously expressed on single cells and within one antigen molecule was reinvestigated by using polysaccharide-specific murine monoclonal antibodies. monoclonal antibodies were generated to the m antigen of brucella melitensis 16m. chemically defined lipopolysaccharides and o polysaccharides from brucella abortus 1119-3, b. melitensis 16m, and yersinia enterocolitica o:9 were used to dissect the binding profiles of the b. melitensis antibodies ... | 1989 | 2474505 |
| [a simplified method of determining the serologic variants of yersinia enterocolitica]. | identification of the serological variants according to g. wauters et al. involves examination of one strain in the agglutination test with 32 sera, and to specify a number of serological variants, investigations with monosera are necessary. six group sera are suggested for the determination of the minimal set of monovalent sera necessary for the identification of the tested strain serological variant. employment of group sera considerably simplifies the serologic identification of the strains. ... | 1989 | 2477611 |
| analysis of brucella lipopolysaccharide with specific and cross-reacting monoclonal antibodies. | monoclonal antibodies which bind brucella a lipopolysaccharide (lps)-specific, m lps-specific, or cross-reactive epitopes were used as reagents in quantitative dot blot, western blot (immunoblot), and immunoprecipitation analysis of brucella whole cells, whole-cell extracts, and purified lps preparations. this set of monoclonal antibodies detected four unique epitopes on brucella lps. the specificity of monoclonal antibodies reactive with brucella unique (a and m) and common (c and c/y) lps epit ... | 1989 | 2479655 |
| [immunoelectrophoretic analysis of the antigenic composition of yersinia]. | the antigenic composition of 24. y. pseudotuberculosis newly isolated and reference strains, 7 y. enterocolitica strains, as well as y. pestis vaccine strain ev, has been studied by the method of immunoelectrophoresis in agar. the antigenic composition of these bacteria has been found to be complicated and to comprise not less than 8-11 antigens, and among them nonspecific protein antigens common for enterobacteria, the common generic antigen, the antigen common with y. pestis, as well as o-anti ... | 1989 | 2480038 |
| [isolation of yersinia enterocolitica in various conditions of cultivation]. | the rates of yersinia isolation from 156 samples of pathologic material cultivated by two methods, the routine one, with the selection of yersinia from a microbial association by prolonged cooling of the examined material in slightly alkaline buffer and multiple inoculations into endo's medium, and the technique suggested by the authors--a rapid method based on the treatment of the biologic material with a concentrated alkaline-salt mixture at 4 degrees c for 2 days and a single inoculation into ... | 1989 | 2481080 |
| [coagglutinating diagnostic agents for serological identification of yersinia enterocolitica]. | coagglutinating antibody agents of y. enterocolitica serologic variants o3; o5; o5.27; o6.30; o7.8; o9; o16 were prepared on the basis of cowan i lyophilized reagent; they were lyophilized with faibich lyophilization medium. these diagnostic agents have shown high activity and specificity. | 1989 | 2481081 |
| [preparation of hyperimmune sera to yersinia enterocolitica]. | the authors describe one of the modifications of the method for the preparation of rabbit sera hyperimmune to y. enterocolitica by intracutaneous injections (into 5 sites of the back) of the antigen with freund's adjuvant, followed by intravenous revaccinations. the antigen for immunization has been prepared by heating yersinia suspension (1.10(6) bacterial cells per ml) in boiling water bath for 2 hrs. the course of a rabbit immunization takes approximately 3.10(6) bacterial cells. antibody tit ... | 1989 | 2481141 |
| [the pharmacological effect of yersinia enterocolitica (o) antigens on isolated guinea pig and rabbit ileum]. | in this study, acetylcholine-like effect of yersinia enterocolitica (o) antigen on isolated guinea-pig and rabbit ileum was observed. atropine was shown its antagonist. | 1989 | 2483247 |
| [stationary and migratory avifauna as reservoirs of salmonella, yersinia and campylobacter]. | domestic and wild animals have been always considered very important as reservoir of agents of human infections. particularly birds, because of their great mobility from a continent to another or within the limits of the same ecosystem may transfer pathogenic micro-organisms. the present survey was undertaken in order to evaluate the presence of campylobacter, yersinia and salmonella in migratory and permanent birds. during the period october 1986 to march 1988 intestinal loops were collected fr ... | 1989 | 2483644 |
| [current problems in the microbiological control of food]. | the paper deals with a number of aspects of current interest and significance on the subject of the microbiological testing of food with particular reference to the role of research institutes. the main objectives of microbiological testing of food are essentially two: (i) to establish the absence of a human health hazard due to microbial contamination of food (food safety); and (ii) to define the quality standard of food (food quality). as far as the first of these objectives is concerned, the ... | 1989 | 2483651 |
| [gram-negative flora of horticultural produce destined for consumption mainly in the raw state]. | a survey has been carried out to evaluate the recovery of enterobacteriaceae in freshly consumed horticultural products. 64 samples of these vegetables random chosen in different stores in the general vegetable market of ferrara have been examined among the families of compositae (lettuce, prickly lettuce, cabbage lettuce, common chicory, artichoke), umbrelliferae (curly parsley, carrot, celery, fennel), cruciferae (garden cabbage, red radish), liliaceae (onion), and solanaceae (tomato). 654 bac ... | 1989 | 2483908 |
| [yersinia enterocolitica in fresh and packaged meats]. | in these last years it was observed an increased frequency of human infections by y. enterocolitica supported mostly in europe by serotype 0:3 and 0:9, and by serotype 0:8 in u.s.a. as far as it is concerned the transmission form to man, in addition to the possible infection between men, the one from animal to man and the one consequent on water use, most important suspicions are about foods derived from infected animals or however contaminated by man (fresh or preserved or processed meat, milk ... | 1989 | 2484479 |
| [yersinia and infantile gastroenteritis]. | fecal samples and intestinal mucosal biopsies of 16 patients in paediatric age, with protracted diarrhoea with blood and mucus, were examined for intestinal pathogens including yersinia. yersinia enterocolitica 1/5/xz and yersinia intermedia 1/18/xz were isolated from fecal samples of two patients. in another child, yersinia enterocolitica, 1/5/xz was isolated from fecal sample, while yersinia enterocolitica 1/self-agglutinable/xz from colon biopsy. the other intestinal pathogens were absent; ae ... | 1989 | 2490392 |
| [bacterial etiologies of acute infantile diarrhea in dakar]. | systematic research into all the bacterial etiological agents responsible for acute diarrhoea was undertaken on 276 diarrhoea stools collected from children aged 0-5 years. particular attention was given to finding bacterial agents usually overlooked during routine checks: campylobacter, vibrionaceae and yersinia enterocolitica. | 1989 | 2491376 |
| lack of effect by prostaglandin f2 alpha and verapamil on calcium uptake by isolated corpora lutea from pseudopregnant rats. | | 1989 | 2492809 |