| anti-infective potential of marine invertebrates and seaweeds from the brazilian coast. | this manuscript describes the evaluation of anti-infective potential in vitro of organic extracts from nine sponges, one ascidian, two octocorals, one bryozoan, and 27 seaweed species collected along the brazilian coast. antimicrobial activity was tested against staphylococcus aureus (atcc 25923), enterococcus faecalis (atcc 29212), pseudomonas aeruginosa (atcc 27853), escherichia coli (atcc 25922) and candida albicans (atcc 10231) by the disk diffusion method. antiprotozoal activity was evaluat ... | 2013 | 23681060 |
| identification of a polysaccharide produced by the pyruvate overproducer candida glabrata cctcc m202019. | candida glabrata has great potential for the accumulation of pyruvate as a preferred strain in pyruvate production by fermentation. however, its substrate conversion rate is relatively low. in this study, a novel polysaccharide containing α-1,4-glucosidic bonds was observed accidentally in screening a high-titer pyruvate strain by atmospheric and room temperature plasma mutagenesis of c. glabrata. chemical analysis of the partially purified polysaccharide s4-c10 showed the main components were 1 ... | 2017 | 28343242 |
| molecular characterization of a novel isolate of candida tropicalis for enhanced lipid production. | to characterize a new isolate of candida tropicalis for its enhanced storage lipid accumulation with respect to lipid composition, fatty acid profile and transcriptional regulation of four key genes involved in lipid productivity using different carbon sources. | 2013 | 23311514 |
| chlamydia pneumoniae clinical isolate from gingival crevicular fluid: a potential atherogenic strain. | chlamydia pneumoniae has been associated to atherosclerotic cardiovascular diseases. the aim of our study was to characterize, for the first time, a c. pneumoniae strain isolated from the gingival crevicular fluid of a patient with chronic periodontitis, described as a risk factor for cardiovascular diseases. c. pneumoniae isolate was characterized and compared to the respiratory ar-39 strain by vd4-ompa genotyping and by investigating the intracellular growth in epithelial and macrophage cell l ... | 2015 | 26636048 |
| difference in distribution of chlamydia trachomatis genotypes among different provinces: a pilot study from four provinces in china. | the objective of this study was to assess the distribution of chlamydia trachomatis genovars in male sexually transmitted disease (std) clinic patients from 1 province in eastern china (jiangsu) and 3 provinces in southern china (guangdong, guangxi, and hainan). urine specimens (n = 140) were collected from 46 public std clinics in the 4 provinces. specimens that tested positive for c. trachomatis by polymerase chain reaction were genotyped for the infecting c. trachomatis strain by amplifying a ... | 2013 | 23429090 |
| fluoroquinolone and macrolide exposure predict clostridium difficile infection with the highly fluoroquinolone- and macrolide-resistant epidemic c. difficile strain bi/nap1/027. | antibiotics have been shown to influence the risk of infection with specific clostridium difficile strains as well as the risk of c. difficile infection (cdi). we performed a retrospective case-control study of patients infected with the epidemic bi/nap1/027 strain in a u.s. hospital following recognition of increased cdi severity and culture of stools positive by c. difficile toxin immunoassay. between 2005 and 2007, 72% (103/143) of patients with first-episode cdis were infected with the bi st ... | 2015 | 26525793 |
| a modified r-type bacteriocin specifically targeting clostridium difficile prevents colonization of mice without affecting gut microbiota diversity. | clostridium difficile is a leading cause of nosocomial infections worldwide and has become an urgent public health threat requiring immediate attention. epidemic lineages of the bi/nap1/027 strain type have emerged and spread through health care systems across the globe over the past decade. limiting person-to-person transmission and eradicating c. difficile, especially the bi/nap1/027 strain type, from health care facilities are difficult due to the abundant shedding of spores that are impervio ... | 2015 | 25805733 |
| real-time polymerase chain reaction method for detection of toxigenic clostridium difficile from stools and presumptive identification of nap1 clone. | this study describes the development of a cost-effective, multiplex real-time polymerase chain reaction (rtpcr) method for detection of toxigenic clostridium difficile from stools and presumptive identification of the nap-1 strain. the diagnostic value of the new method is for the detection of toxigenic c. difficile which has the following performance characteristics: 99.8% specificity, 95.1% sensitivity, 97.5% positive predictive value, and 99.5% negative predictive value. examination of 24 spe ... | 2013 | 23182075 |
| clostridium botulinum type d/c intoxication in a dairy cow stock in saxony-anhalt (germany)--report on an innovative diagnostic approach. | botulism in cattle is a rare but serious disease. in germany there is no obligation to report botulism in animals and therefore a precise morbidity rate is not available. in this manuscript we describe an outbreak of clostridium (c.) botulinum neurotoxin (bont) intoxication in a saxony-anhalt dairy cow stock of 286 holstein-friesian cows and offspring in spring/summer 2009 and its diagnostic approach. 122 animals showed clinical signs of bont intoxication. 115 of the affected animals (40.2% of t ... | 2016 | 27169148 |
| genetic profiles of propionibacterium acnes and identification of a unique transposon with novel insertion sequences in sarcoid and non-sarcoid isolates. | propionibacterium acnes is one of the most commonly implicated etiologic agents of sarcoidosis. we previously reported a complete genome sequence of the c1 strain of p. acnes as a clinical isolate from subcutaneous granulomatous inflammatory lesions in a patient with sarcoidosis. in the present study, we initially searched for genetic profiles specific to the c1 strain by core genome analysis and multiple genome alignment with database sequences from 76 and 9 p. acnes strains, respectively. the ... | 2015 | 25966054 |
| characterization of polyhydroxyalkanoate (pha) synthase derived from delftia acidovorans ds-17 and the influence of pha production in escherichia coli. | heterologous expression of polyhydroxyalkanoate (pha) synthase from delftia acidovorans ds-17 (phac(da)) in escherichia coli jm109 leads to effective production of high-molecular-weight poly[(r)-3-hydroxybutyrate] [p(3hb)]. this study examined the effect of phac(da) expression on p(3hb) production in e. coli jm109 (da strain) by comparing with the strain expressing pha synthase (phac(re)) from ralstonia eutropha (re strain). first, the kinetic properties of phac(da) were investigated. among the ... | 2013 | 23333645 |
| whole genome sequencing-based molecular epidemiologic analysis of autochthonous dengue virus type 1 strains circulating in japan in 2014. | cases of autochthonous infections of dengue virus type 1 (denv-1) were detected in japan after a 70-year period devoid of dengue outbreaks. we previously showed that e gene sequences are identical in 11 of the 12 denv-1 strains autochthonous to japan. however, the e sequence represents only 14% of the denv-1 genome. in the present study, we have sequenced the entire genome of 6 autochthonous denv-1 strains that were isolated from patients during the 2014 outbreak. sequencing of 5 yoyogi group st ... | 2017 | 27169954 |
| experimental infection of cattle with ovine dichelobacter nodosus isolates. | dichelobacter nodosus is the main causative agent of ovine footrot, and there are strong indications that the bacterium can be transferred to cattle grazing on the same pasture as sheep. the aim of this study was to investigate if benign and virulent d. nodosus strains isolated from sheep can be transferred to the interdigital skin of cattle under experimental conditions. further, we wanted to observe the impact of such infection on bovine foot health, and test the effect of topical chlortetracy ... | 2015 | 26407552 |
| a novel library-independent approach based on high-throughput cultivation in bioscreen and fingerprinting by ftir spectroscopy for microbial source tracking in food industry. | microbiological source tracking (mst) for food industry is a rapid growing area of research and technology development. in this paper, a new library-independent approach for mst is presented. it is based on a high-throughput liquid microcultivation and ftir spectroscopy. in this approach, ftir spectra obtained from micro-organisms isolated along the production line and a product are compared to each other. we tested and evaluated the new source tracking approach by simulating a source tracking s ... | 2017 | 27783405 |
| oral and conjunctival exposure of nonhuman primates to low doses of ebola makona virus. | nonhuman primate (nhp) models of ebola virus (ebov) infection primarily use parenteral or aerosol routes of exposure. uniform lethality can be achieved in these models at low doses of ebov (≤100 plaque-forming units [pfu]). here, we exposed nhps to low doses of ebov (makona strain) by the oral or conjunctival routes. surprisingly, animals exposed to 10 pfu by either route showed no signs of disease. exposure to 100 pfu resulted in illness and/or lethal infection. these results suggest that these ... | 2016 | 27284090 |
| combination of cell culture and qpcr to assess the efficacy of different anticoccidials on eimeria tenella sporozoites. | three in vitro studies were designed to develop an assay for anticoccidial efficacy by use of laboratory (houghton) and field (t-376) eimeria tenella strains. in study (1), minimum inhibitory concentrations (mics) of monensin (mon), maduramicin (mad), salinomycin (sal), and lasalocid (las) were determined that are able to inhibit more than 50% of sporozoites in host cell (madin-darby bovine kidney (mdbk)) penetration and more than 95% of houghton sporozoites development to mature merozoites (tre ... | 2015 | 25773180 |
| functional characterizations of malonyl-coa:acyl carrier protein transacylase (mcat) in eimeria tenella. | eimeria tenella, an apicomplexan parasite in chickens, possesses an apicoplast and its associated metabolic pathways including the type ii fatty acid synthesis (fas ii). malonyl-coa:acyl-carry protein transacylase (mcat) encoded by the fabd gene is one of the essential enzymes in the fas ii system. in the present study, the entire e. tenella mcat gene (etfabd) was cloned and sequenced. immunolabeling located this protein in the apicoplast organelle in coccidial sporozoites. functional replacemen ... | 2012 | 22525053 |
| construction and evaluation of an exopolysaccharide-producing engineered bacterial strain by protoplast fusion for microbial enhanced oil recovery. | enterobacter cloacae strain jd, which produces water-insoluble biopolymers at optimal temperature of 30°c, and a thermophilic geobacillus strain were used to construct an engineered strain for exopolysaccharide production at high temperatures by protoplast fusion. the obtained fusant strain zr3 produced exopolysaccharides at up to 45°c with optimal growth temperature at 35°c. the fusant produced exopolysaccharides of approximately 7.5 g/l or more at ph between 7.0 and 9.0. the feasibility of the ... | 2013 | 23856587 |
| ph-induced change in cell susceptibility to butanol in a high butanol-tolerant bacterium, enterococcus faecalis strain cm4a. | though butanol is considered as a potential biofuel, its toxicity toward microorganisms is the main bottleneck for the biological butanol production. recently, butanol-tolerant bacteria have been proposed as alternative butanol production hosts overcoming the end product inhibition. one remaining key issue to be addressed is how physicochemical properties such as ph and temperature affect microbial butanol tolerance during cultivation and fermentation. | 2015 | 25904984 |
| the multi-targeted kinase inhibitor sorafenib inhibits enterovirus 71 replication by regulating ires-dependent translation of viral proteins. | the activation of erk and p38 signal cascade in host cells has been demonstrated to be essential for picornavirus enterovirus 71 (ev71) replication and up-regulation of virus-induced cyclooxygenase-2 (cox-2)/prostaglandins e2 (pge2) expression. the aim of this study was to examine the effects of sorafenib, a clinically approved anti-cancer multi-targeted kinase inhibitor, on the propagation and pathogenesis of ev71, with a view to its possible mechanism and potential use in the design of therapy ... | 2014 | 24680956 |
| synthetic genome engineering forging new frontiers for wine yeast. | over the past 15 years, the seismic shifts caused by the convergence of biomolecular, chemical, physical, mathematical, and computational sciences alongside cutting-edge developments in information technology and engineering have erupted into a new field of scientific endeavor dubbed synthetic biology. recent rapid advances in high-throughput dna sequencing and dna synthesis techniques are enabling the design and construction of new biological parts (genes), devices (gene networks) and modules ( ... | 2017 | 27535766 |
| an outbreak of type π vaccine-derived poliovirus in sichuan province, china: emergence and circulation in an under-immunized population. | during august 2011-february 2012, an outbreak of type π circulating vaccine-derived poliovirus (cvdpvs) occurred in sichuan province, china. | 2014 | 25503964 |
| outbreak of paralytic poliomyelitis in oman: evidence for widespread transmission among fully vaccinated children. | from january, 1988, to march, 1989, a widespread outbreak (118 cases) of poliomyelitis type 1 occurred in oman. incidence of paralytic disease was highest in children younger than 2 years (87/100,000) despite an immunisation programme that recently had raised coverage with 3 doses of oral poliovirus vaccine (opv) among 12-month-old children from 67% to 87%. we did a case-control study (70 case-patients, 692 age-matched controls) to estimate the clinical efficacy of opv, assessed the immunogenici ... | 1991 | 1679866 |
| structural and biochemical insights into the v/i505t mutation found in the eiav gp45 vaccine strain. | the equine infectious anemia virus (eiav) is a lentivirus of the retrovirus family, which causes persistent infection in horses often characterized by recurrent episodes of high fever. it has a similar morphology and life cycle to the human immunodeficiency virus (hiv). its transmembrane glycoprotein, gp45 (analogous to gp41 in hiv), mediates membrane fusion during the infection. however, the post-fusion conformation of eiav gp45 has not yet been determined. eiav is the first member of the lenti ... | 2014 | 24656154 |
| single nucleotide polymorphism genotyping of erysipelothrix rhusiopathiae isolates from pigs affected with chronic erysipelas in japan. | over the past decades, erysipelothrix rhusiopathiae strains displaying similar phenotypic and genetic profiles of the attenuated, acriflavine-resistant e. rhusiopathiae koganei 65-0.15 strain (serovar 1a) have been frequently isolated from pigs affected with chronic erysipelas in japan. in this study, using the conventional pcr assay that was designed to detect strain-specific single nucleotide polymorphism (snp) sites found in the genome of the vaccine strain, we analyzed e. rhusiopathiae isola ... | 2017 | 28250289 |
| role of surface protective antigen a in the pathogenesis of erysipelothrix rhusiopathiae strain c43065. | to clarify the role of surface protective antigen a (spaa) in the pathogenesis of erysipelothrix rhusiopathiae c43065 (serotype 2), the spaa deletion mutant of e. rhusiopathiae δspaa was constructed by homologous recombination. the virulence of the δspaa mutant decreased more than 76-fold compared with that of the wild-type strain c43065 in mice. the mutant strain was sensitive to the bactericidal action of swine serum, whereas the wild-type strain was resistant. the adhesion of wild-type strain ... | 2015 | 25223326 |
| capsular polysaccharide of erysipelothrix rhusiopathiae, the causative agent of swine erysipelas, and its modification with phosphorylcholine. | the capsule has been implicated in the virulence of the swine pathogen erysipelothrix rhusiopathiae, a rod-shaped, intracellular gram-positive bacterium that has a unique phylogenetic position in the phylum firmicutes and is a close relative of mollicutes (mycoplasma species). in this study, we analyzed the genetic locus and composition of the capsular polysaccharide (cps) of the fujisawa strain of e. rhusiopathiae. genome analysis of the fujisawa strain revealed that the genetic locus for capsu ... | 2012 | 22949554 |
| role of the srrz/rz1 lambdoid lysis cassette in the pathoadaptive evolution of shigella. | shigella, the etiological agent of bacillary dysentery (shigellosis), is a highly adapted human pathogen. it evolved from an innocuous ancestor resembling the escherichia coli strain by gain and loss of genes and functions. while the gain process concerns the acquisition of the genetic determinants of virulence, the loss is related to the adaptation of the genome to the new pathogenic status and occurs by pathoadaptive mutation of antivirulence genes. in this study, we highlight that the srrz/rz ... | 2017 | 28389211 |
| selenium-mediated protection in reversing the sensitivity of bacterium to the bactericidal antibiotics. | inducing production of damaging reactive oxygen species (ros) is an important criterion to distinguish the bactericidal antibiotics from bacteriostatic antibiotics. selenoenzymes were generally recognized to be a powerful antioxidant capable of scavenging free radicals, protecting the cells from the harmful effects of ros. therefore, the present study was carried out to investigate the selenium (se)-mediated protection in reversing antibiotic sensitivity and the role of selenoenzymes in alleviat ... | 2017 | 28347459 |
| immunogenicity of amino acids 1-150 of streptococcus gapc displayed on the surface of escherichia coli. | streptococcus is one of the main pathogens that cause bovine mastitis. they includes into s.agalactiae, s.dysgalactiae, and s.uberis. the gapc protein is a virulence factor that is expressed on the surface of streptococcus species. gapc is highly antigenic and immunization with gapc confers cross-protection against all three species. our previous data showed that amino acids 1-150 of gapc (gapc1-150) of s. dysgalactiae conferred similar immunoprotection compared to full-length gapc. thus, the pr ... | 2017 | 28179116 |
| pyruvate dehydrogenase complex regulator (pdhr) gene deletion boosts glucose metabolism in escherichia coli under oxygen-limited culture conditions. | pyruvate dehydrogenase complex regulator (pdhr) is a transcriptional regulator that negatively regulates formation of pyruvate dehydrogenase complex (pdhc), nadh dehydrogenase (ndh)-2, and cytochrome bo3 oxidase in escherichia coli. to investigate the effects of a pdhr defect on glucose metabolism, a pdhr deletion mutant was derived from the wild-type e. coli w1485 strain by λ red-mediated recombination. while no difference in the fermentation profiles was observed between the two strains under ... | 2017 | 28007420 |
| potential swimming motility variation by acrr in escherichia coli. | acrr, the toxic-compounds-response regulator, regulates motility in microorganisms, presumably to escape from toxic environments. in this study, the genome-wide target genes of acrr were investigated in a δacrr mutant strain by microarray analysis. in the absence of acrr, the transcription of most flagella/motility genes was highly increased. in addition, flagella formation was increased in this mutant strain. motility assays revealed that acrr modulates swimming motility, but not swarming. | 2016 | 27558437 |
| a new metabolic route for the production of gamma-aminobutyric acid by corynebacterium glutamicum from glucose. | gamma-aminobutyric acid (gaba), a non-protein amino acid widespread in nature, is a component of pharmaceuticals, foods, and the biodegradable plastic polyamide 4. corynebacterium glutamicum shows great potential for the production of gaba from glucose. gaba added to the growth medium hardly affected growth of c. glutamicum, since a half-inhibitory concentration of 1.1 m gaba was determined. as alternative to gaba production by glutamate decarboxylation, a new route for the production of gaba vi ... | 2016 | 27289384 |
| metabolic engineering of escherichia coli for astragalin biosynthesis. | astragalin (kaempferol 3-o-glucoside) is used as a standard to assess the quality of radix astragali and has exhibited a number of biological properties. in this work, we screened several udp-dependent glycosyltransferases (ugt) for their potential as efficient biocatalysts for astragalin synthesis. the highest astragalin production with 285 mg/l was detected in the recombinant strain expressing ugt from arabidopis thaliana (atugt78d2). to further improve astragalin production, an efficient udp- ... | 2016 | 27730814 |
| cloning, expression, and purification of pseudomonas aeruginosa flagellin, and characterization of the elicited anti-flagellin antibody. | pseudomonas aeruginosa is an important opportunistic human pathogen that causes serious infections in immunocompromised hosts. the single polar flagellum is an important factor in both virulence and colonization. | 2016 | 27621933 |
| construction of a cyanobacterium synthesizing cyclopropane fatty acids. | microalgae have received much attention as a next-generation source of biomass energy. however, most of the fatty acids (fas) from microalgae are multiply unsaturated; thus, the biofuels derived from them are fluid, but vulnerable to oxidation. in this study, we attempted to synthesize cyclopropane fas in the cyanobacterium synechocystis sp. pcc 6803 by expressing the cfa gene for cyclopropane fa synthase from escherichia coli with the aim of producing fas that are fluid and stable in response t ... | 2016 | 27263419 |
| pathway construction and metabolic engineering for fermentative production of ectoine in escherichia coli. | ectoine is a protective agent and stabilizer whose synthesis pathway exclusively exists in select moderate halophiles. a novel established process called "bacterial milking" efficiently synthesized ectoine in moderate halophiles, however, this method places high demands on equipment and is cost prohibitive. in this study, we constructed an ectoine producing strain by introducing the ectoine synthesis pathway into escherichia coli and improved its production capacity. firstly, the ectabc gene clu ... | 2016 | 26969253 |
| alteration in apyrase enzyme attenuated virulence of shigella flexneri. | virulence of shigella is attributed to the genes presence in chromosome or in the megaplasmid. the apy gene which is located in the megaplasmid of shigella species encodes for apyrase enzyme, a pathogenesis-associated enzyme causing mitochondrial damage and host cell death. in this study we constructed an apy mutant of shigella flexneri by insertional activation using a kanamycin resistant gene cassette. the wild type apy gene of s. flexneri 2a was pcr amplified, cloned and mutated with insertio ... | 2016 | 26706344 |
| effective treatment of hypertension by recombinant lactobacillus plantarum expressing angiotensin converting enzyme inhibitory peptide. | hypertension is considered the most serious risk factor for cardiovascular disease. angiotensin-converting enzyme inhibitory peptides (aceips), which are made from tuna frame protein (tfp) and yellow fin sole frame protein (yfp), have been used previously to treat hypertension. however, the production of these short peptides is usually dependent on enzymatic hydrolysis, resulting in a digested mixture that makes it difficult to purify the aceips. although it has been reported that aceips could b ... | 2015 | 26691527 |
| cranberry (vaccinium macrocarpon) oligosaccharides decrease biofilm formation by uropathogenic escherichia coli. | the preventive effects of the american cranberry (vaccinium macrocarpon) against urinary tract infections are supported by extensive studies which have primarily focused on its phenolic constituents. herein, a phenolic-free carbohydrate fraction (designated cranf1b-f2) was purified from cranberry fruit using ion exchange and size exclusion chromatography. maldi-tof-ms analysis revealed that the cranf1b-f2 constituents are predominantly oligosaccharides possessing various degrees of polymerisatio ... | 2015 | 26613004 |
| understanding the virulence of the entero-aggregative e. coli o104:h4. | o104:h4 is a new strain of e. coli that has caused an outbreak in germany. it was isolated from patients with bloody diarrhoea and haemolytic uremic syndrome (hus). bgi (www.genomics.cn) sequenced and assembled this new strain. it was reported to show resistance to a number of drugs that are toxic to other e. coli and causes serious complications during infections, which ultimately lead to death. multi-drug resistance and high virulence of this strain is thought to be acquired from different sou ... | 2015 | 26561016 |
| enhanced production of l-sorbose in an industrial gluconobacter oxydans strain by identification of a strong promoter based on proteomics analysis. | gluconobacter oxydans is capable of rapidly incomplete oxidation of many sugars and alcohols, which means the strain has great potential for industrial purposes. strong promoters are one of the essential factors that can improve strain performance by overexpression of specific genes. in this study, a pipeline for screening strong promoters by proteomics analysis was established. based on the procedure, a new strong promoter designated as p b932_2000 was identified in g. oxydans wsh-003. the prom ... | 2015 | 25952118 |
| adhesive properties of yapv and paralogous autotransporter proteins of yersinia pestis. | yersinia pestis is the causative agent of plague. this bacterium evolved from an ancestral enteroinvasive yersinia pseudotuberculosis strain by gene loss and acquisition of new genes, allowing it to use fleas as transmission vectors. infection frequently leads to a rapidly lethal outcome in humans, a variety of rodents, and cats. this study focuses on the y. pestis kim yapv gene and its product, recognized as an autotransporter protein by its typical sequence, outer membrane localization, and am ... | 2015 | 25690102 |
| novel leptospira interrogans protein lsa32 is expressed during infection and binds laminin and plasminogen. | pathogenic leptospira is the aetiological agent of leptospirosis, a life-threatening disease of human and veterinary concern. the quest for novel antigens that could mediate host-pathogen interactions is being pursued. owing to their location, these antigens have the potential to elicit numerous activities, including immune response and adhesion. this study focuses on a hypothetical protein of leptospira, encoded by the gene lic11089, and its three derived fragments: the n-terminal, intermediate ... | 2015 | 25627443 |
| a synthetic suicide riboswitch for the high-throughput screening of metabolite production in saccharomyces cerevisiae. | artificial devices such as the synthetic riboswitch have shown potential to introduce unnatural phenotypic perturbation because its synthetic traits are distinct from that of innate metabolism. in this study, a riboswitch, a small regulatory element found in rnas, was employed to reprogram microorganisms to produce valuable metabolites. a self-cleaving ribozyme glms, found in gram-positive bacteria, cleaves its own transcript in response to the intracellular glucosamine 6-phosphate (glcn6p) conc ... | 2015 | 25596509 |
| incp-1β plasmids are important carriers of fitness traits for variovorax species in the mycosphere--two novel plasmids, phb44 and pbs64, with differential effects unveiled. | the laccaria proxima mycosphere strongly selects variovorax paradoxus cells. fifteen independent v. paradoxus strains, isolated from mycospheres sampled at two occasions, were investigated with respect to the occurrence of plasmids of sizes <60-100 kb. two v. paradoxus strains, hb44 and bs64, were found to contain such plasmids, which were coined phb44 and pbs64. replicon typing using a suite of plasmid-specific pcr systems indicated that both plasmids belong to the incp-1β group. also, both wer ... | 2015 | 25542203 |
| engineering an iterative polyketide pathway in escherichia coli results in single-form alkene and alkane overproduction. | alkanes and alkenes are ideal biofuels, due to their high energy content and ability to be safely transported. to date, fatty acid-derived pathways for alkane and alkene bioproduction have been thoroughly explored. in this study, we engineered the pathway of the iterative type i polyketide synthase (pks) sgce with the cognate thioesterase (te) sgce10 in escherichia coli, with the goal of overproducing pentadecaheptaene (pdh) followed by its hydrogenation to pentadecane (pd). based on initial in ... | 2015 | 25536488 |
| systematically engineering escherichia coli for enhanced production of 1,2-propanediol and 1-propanol. | the biological production of high value commodity 1,2-propanediol has been established by engineering the glycolysis pathway. however, the simultaneous achievement of high titer and high yield has not been reported yet, as all efforts in increasing the titer have resulted in low yields. in this work, we overcome this limitation by employing an optimal minimal set of enzymes, channeling the carbon flux into the 1,2-propanediol pathway, increasing nadh availability, and improving the anaerobic gro ... | 2015 | 25490349 |
| [effect of stx2-encoding phage on the motility and gene expression involved in moving of escherichia coli lysogen]. | the effect of flhdc, flia, flid and flie genes involved in moving of escherichia coli (e. coli) on the motility of lysogened strain by stx2-encoding phage phimin27 was explored by gene knockout and phage lysogenic conversion. | 2014 | 25252454 |
| the role of the chi1 gene from the endophytic bacteria serratia proteamaculans 336x in the biological control of wheat take-all. | take-all, a disease caused by the fungus gaeumannomyces graminis var. tritici, is the most important root disease of wheat and causes severe yield losses worldwide. using microorganisms as biological agents to control the disease is important because no resistant cultivars or effective chemical fungicides are available. in this study, we tested the biological control capability of a chitinase produced by the endophytic bacterium serratia proteamaculans 336x against wheat take-all. the chitinase ... | 2014 | 25093749 |
| efficacy of a live attenuated escherichia coli o78:k80 vaccine in chickens and turkeys. | a candidate live vaccine for avian pathogenic escherichia coli (apec) was constructed from a virulent field apec 078 strain by mutation of the aroa gene. the mutant was highly similar to the parent wild-type strain in respect of colony morphology, motility, growth in suspension, hemagglutination, congo red binding, hep-2 cell adhesion, and the elaboration of surface antigens type 1 fimbriae and flagella, although production of curli fimbriae was reduced marginally. the mutant proved avirulent wh ... | 2013 | 24689185 |
| [rmla gene deletion affects biofilm formation by avian pathogenic escherichia coli]. | to characterize rmla gene inavian pathogenic escherichia coli (apec). | 2013 | 24409761 |
| an improved escherichia coli strain to host gene regulatory networks involving both the arac and laci inducible transcription factors. | many of the gene regulatory networks used within the field of synthetic biology have extensively employed the arac and laci inducible transcription factors. however, there is no escherichia coli strain that provides a proper background to use both transcription factors simultaneously. we have engineered an improved e. coli strain by knocking out the endogenous laci from a strain optimal for arac containing networks, and thoroughly characterized the strain both at molecular and functional levels. ... | 2014 | 24382032 |
| expression and purification of recombinant rop1 of toxoplasma gondii in bacteria. | toxoplasmosis is a worldwide-distributed infection which is mostly asymptomatic but can cause serious health problems in congenitally-infected newborns and immunecompromised individuals. research is undergoing both to improve toxoplasma serological tests, which play the main role in laboratory diagnosis of the infection, and develop an effective vaccine to prevent the infection. some studies showed usefulness of rhoptry protein 1 (rop1) antigen of toxoplasma gondii (t. gondii) in serodiagnosis o ... | 2013 | 24285997 |
| construction of a full-length infectious bacterial artificial chromosome clone of duck enteritis virus vaccine strain. | duck enteritis virus (dev) is the causative agent of duck viral enteritis, which causes an acute, contagious and lethal disease of many species of waterfowl within the order anseriformes. in recent years, two laboratories have reported on the successful construction of dev infectious clones in viral vectors to express exogenous genes. the clones obtained were either created with deletion of viral genes and based on highly virulent strains or were constructed using a traditional overlapping fosmi ... | 2013 | 24195756 |
| differentiation of microbial species and strains in coculture biofilms by multivariate analysis of laser desorption postionization mass spectra. | 7.87 to 10.5 ev vacuum ultraviolet (vuv) photon energies were used in laser desorption postionization mass spectrometry (ldpi-ms) to analyze biofilms comprised of binary cultures of interacting microorganisms. the effect of photon energy was examined using both tunable synchrotron and laser sources of vuv radiation. principal components analysis (pca) was applied to the ms data to differentiate species in escherichia coli-saccharomyces cerevisiae coculture biofilms. pca of ldpi-ms also different ... | 2013 | 24067765 |
| metabolic engineering for l-glutamine overproduction by using dna gyrase mutations in escherichia coli. | an l-glutamine-overproducing mutant of an escherichia coli k-12-derived strain was selected from randomly mutagenized cells in the course of l-alanyl-l-glutamine strain development. genome-wide mutation analysis unveiled a novel mechanism for l-glutamine overproduction in this mutant. three mutations were identified that are related to the l-glutamine overproduction phenotype, namely, an intergenic mutation in the 5'-flanking region of yeig and two nonsynonymous mutations in gyra (gly821ser and ... | 2013 | 23455340 |
| [gene cloning, expression and polyclonal antibody preparation and application of translocated intimin receptor-cytoskeleton coupling protein]. | to prepare translocated intimin receptor-cytoskeleton coupling protein (tccp) of enterohaemorrhagic escherichia coli (ehec) o157:h7 and its polyclonal antibody. | 2012 | 23232519 |
| [presence of genes of genotoxin associated with pks pathogenicity island in escherichia coli m-17 probiotic strain]. | study the presence of genetic determinants of pks pathogenicity island containing clb (colibactin) genes in bacteria of the e. coli m-17 production strain by using pcr. | 2012 | 23163031 |
| improved production of l-threonine in escherichia coli by use of a dna scaffold system. | despite numerous approaches for the development of l-threonine-producing strains, strain development is still hampered by the intrinsic inefficiency of metabolic reactions caused by simple diffusion and random collisions of enzymes and metabolites. a scaffold system, which can promote the proximity of metabolic enzymes and increase the local concentration of intermediates, was reported to be one of the most promising solutions. here, we report an improvement in l-threonine production in escheric ... | 2013 | 23160128 |
| substitutions in the escherichia coli rna polymerase inhibitor t7 gp2 that allow inhibition of transcription when the primary interaction interface between gp2 and rna polymerase becomes compromised. | the escherichia coli-infecting bacteriophage t7 encodes a 7 kda protein, called gp2, which is a potent inhibitor of the host rna polymerase (rnap). gp2 is essential for t7 phage development. the interaction site for gp2 on the e. coli rnap is the β' jaw domain, which is part of the dna binding channel. the binding of gp2 to the β' jaw antagonizes several steps associated with interactions between the rnap and promoter dna, leading to inhibition of transcription at the open promoter complex forma ... | 2012 | 22977089 |
| [influence of corynebacteria metabolites on antagonistic activity of h2o2 producing lactobacilli]. | study combined influence of corynebacterium genus bacteria metabolites and h2o2 producing lactobacilli on survival rate of staphylococcus aureus, escherichia coli and lactobacillus acidophilus. | 2013 | 22937706 |
| a histone-like protein of helicobacter pylori protects dna from stress damage and aids host colonization. | genomic dna in a bacterial cell is folded into a compact structure called a nucleoid, and nucleoid-associated proteins are responsible for proper assembly of active higher-order genome structures. the human gastric pathogen helicobacter pylori express a nucleoid-associated protein encoded by the hup gene, which is the homolog to the escherichia coli histone-like protein hu. an h. pylori hup mutant strain (x47 hup:cat) showed a defect in stationary phase survival. the x47 hup:cat mutant was more ... | 2012 | 22776439 |
| lsa30, a novel adhesin of leptospira interrogans binds human plasminogen and the complement regulator c4bp. | pathogenic leptospira is the etiological agent of leptospirosis, a life-threatening disease that affects populations worldwide. surface proteins have the potential to promote several activities, including adhesion. this work aimed to study the leptospiral coding sequence (cds) lic11087, genome annotated as hypothetical outer membrane protein. the lic11087 gene was cloned and expressed in escherichia coli bl21 (de3) strain by using the expression vector pae. the recombinant protein tagged with n- ... | 2012 | 22732096 |
| biodiesel synthesis by a one-step method in a genetically engineered escherichia coli using rice straw hydrolysate and restaurant oil wastes as raw materials. | to reduce the cost of biodiesel, the utilization of rice straw hydrolysate and restaurant oil wastes as the substrates for biodiesel production in a recombinant strain by a one-step method was investigated. | 2012 | 22681508 |
| french cattle is not a reservoir of the highly virulent enteroaggregative shiga toxin-producing escherichia coli of serotype o104:h4. | in may-june 2011, a massive outbreak of haemolytic uraemic syndrome caused by enteroaggregative shiga toxin (stx)-producing escherichia coli (stec) o104:h4 occurred in europe, which was linked to the consumption of sprouted seeds. as ruminants are known reservoirs of stec, this study investigated whether cattle could be a reservoir of enteroaggregative stec o104:h4 and a potential source of transmission to humans. a total of 1468 french cattle were analysed for faecal carriage of the outbreak st ... | 2012 | 22424867 |
| heterologous transformation of agrocybe aegerita with a bacterial neomycin-resistance gene fused to a fungal promoter-like dna sequence. | dna sequences of the basidiomycete agrocybe aegerita were cloned in e. coli based on their ability to drive the expression of the bacterial promoterless tetracycline (tc)-resistance gene. a 0.48% frequency of the cloned sequences promoted antibiotic-resistance. the sequence conferring the highest tc resistance (40 μg/ml) was selected to drive the expression in e. coli of two other promoterless genes encoding chloramphenicol and neomycin resistance. one of the derivative vectors, pn13-a2, carryin ... | 1995 | 24173057 |
| effect of amino acid mutation at position 127 in 3a of a rabbit-attenuated foot-and-mouth disease virus serotype asia1 on viral replication and infection. | an amino acid mutation (r127→i) in the 3a non-structural protein of an fmdv serotype asia1 rabbit-attenuated zb strain was previously found after attenuation of the virus. to explore the effects of this mutation on viral replication and infection, the amino acid residue isoleucine (i) was changed to arginine (r) in the infectious cdna clone of the rabbit-attenuated zb strain by sitedirected mutagenesis, and the r127-mutated virus was rescued. bhk monolayer cells and suckling mice were inoculated ... | 2014 | 25326348 |
| characterization of a hypervirulent fowl adenovirus 4 with the novel genotype newly prevalent in china and establishment of reproduction infection model of hydropericardium syndrome in chickens. | severe hydropericardium syndrome (hps) has been present in layers in the northeast of china since june 2015, with mortality rates varying from 30 to 90%. dead layers had severe hydropericardium with pericardial volumes of 5 to 20 ml, as well as inclusion body hepatitis. laboratory investigations led to the isolation of a fowl adenovirus strain, hljfad15, from the liver tissue of dead layers. natural deletions of orf19 and orf27 were found in this clinical strain by complete genome sequencing, wh ... | 2017 | 28339951 |
| [construction and properties of the francisella tularensis vaccine strain without one copy of the iglc gene and without reca gene]. | the live vaccine based on the francisella tularensis subsp. holarctica vaccine strain 15 niieg line is used in russia against tularemia. this vaccine is highly effective, but fairly unstable. therefore, development of stable live tularemia vaccine with minimal side effect is rather urgent. the method of allel removal in the f. tularensis vaccine strain was used to remove one copy of the iglc gene, which is required to provide intracellular production of the vaccine strain, as well as removal of ... | 2015 | 26665740 |
| balb/c mice, but not c57bl/6 mice immunized with a δclpb mutant of francisella tularensis subspecies tularensis are protected against respiratory challenge with wild-type bacteria: association of protection with post-vaccination and post-challenge immune responses. | francisella tularensis subspecies tularensis is highly virulent for humans especially when it is inhaled. therefore, it has the potential to be used as a biothreat agent. vaccines against f. tularensis will need to be approved in accordance with the fda animal rule. this will require identification of robust correlates of protection in experimental animals and the demonstration that similar immune responses are generated in vaccinated humans. towards this goal, we have developed an experimental ... | 2012 | 22484348 |
| n2o production, a widespread trait in fungi. | n2o is a powerful greenhouse gas contributing both to global warming and ozone depletion. while fungi have been identified as a putative source of n2o, little is known about their production of this greenhouse gas. here we investigated the n2o-producing ability of a collection of 207 fungal isolates. seventy strains producing n2o in pure culture were identified. they were mostly species from the order hypocreales order-particularly fusarium oxysporum and trichoderma spp.-and to a lesser extent s ... | 2015 | 25894103 |
| revealing in vivo glucose utilization of gluconobacter oxydans 621h δmgdh strain by mutagenesis. | gluconobacter oxydans, belonging to acetic acid bacteria, is widely used in industrial biotechnology. in our previous study, one of the main glucose metabolic pathways in g. oxydans 621h was blocked by the disruption of the mgdh gene, which is responsible for glucose oxidation to gluconate on cell membrane. the resulting 621h δmgdh mutant strain showed an enhanced growth and biomass yield on glucose. in order to further understand the intracellular utilization of glucose by 621h δmgdh, the funct ... | 2014 | 24035043 |
| generation of a reassortant avian influenza virus h5n2 vaccine strain capable of protecting chickens against infection with egyptian h5n1 and h9n2 viruses. | avian influenza h5n1 viruses have been enzootic in egyptian poultry since 2006. avian influenza h9n2 viruses which have been circulating in egyptian poultry since 2011 showed high replication rates in embryonated chicken eggs and mammalian cells. | 2016 | 26620838 |
| generation of live attenuated influenza virus by using codon usage bias. | seasonal influenza epidemics and occasional pandemics threaten public health worldwide. new alternative strategies for generating recombinant viruses with vaccine potential are needed. interestingly, influenza viruses circulating in different hosts have been found to have distinct codon usage patterns, which may reflect host adaptation. we therefore hypothesized that it is possible to make a human seasonal influenza virus that is specifically attenuated in human cells but not in eggs by converti ... | 2015 | 26269186 |
| molecular evolution of the hemagglutinin and neuraminidase genes of pandemic (h1n1) 2009 influenza viruses in sendai, japan, during 2009-2011. | analyzing the evolutionary pattern of the influenza a(h1n1)pdm09 strain in different regions is important for understanding its diversification. we therefore conducted this study to elucidate the genetic variability and molecular evolution of the influenza a(h1n1)pdm09 strains that circulated during the 2009-2010 and 2010-2011 influenza seasons in sendai, japan. nasopharyngeal swab specimens were collected from patients with influenza-like illnesses who visited outpatient clinics in sendai city, ... | 2013 | 24078044 |
| estimated global mortality associated with the first 12 months of 2009 pandemic influenza a h1n1 virus circulation: a modelling study. | 18,500 laboratory-confirmed deaths caused by the 2009 pandemic influenza a h1n1 were reported worldwide for the period april, 2009, to august, 2010. this number is likely to be only a fraction of the true number of the deaths associated with 2009 pandemic influenza a h1n1. we aimed to estimate the global number of deaths during the first 12 months of virus circulation in each country. | 2012 | 22738893 |
| computational prediction of vaccine strains for human influenza a (h3n2) viruses. | human influenza a viruses are rapidly evolving pathogens that cause substantial morbidity and mortality in seasonal epidemics around the globe. to ensure continued protection, the strains used for the production of the seasonal influenza vaccine have to be regularly updated, which involves data collection and analysis by numerous experts worldwide. computer-guided analysis is becoming increasingly important in this problem due to the vast amounts of generated data. we here describe a computation ... | 2014 | 25122778 |
| modified newcastle disease virus vectors expressing the h5 hemagglutinin induce enhanced protection against highly pathogenic h5n1 avian influenza virus in chickens. | naturally-occurring attenuated strains of newcastle disease virus (ndv) are being developed as vaccine vectors for use in poultry and humans. however, some ndv strains, such as beaudette c (bc), may retain too much virulence in poultry for safe use, and more highly attenuated strains may be suboptimally immunogenic. we therefore modified the bc strain by changing the multibasic cleavage site sequence of the f protein to the dibasic sequence of avirulent strain lasota. additionally, the bc, f, an ... | 2014 | 24968158 |
| efficacy of single dose of a bivalent vaccine containing inactivated newcastle disease virus and reassortant highly pathogenic avian influenza h5n1 virus against lethal hpai and ndv infection in chickens. | highly pathogenic avian influenza (hpai) and newcastle disease (nd) are 2 devastating diseases of poultry, which cause great economic losses to the poultry industry. in the present study, we developed a bivalent vaccine containing antigens of inactivated nd and reassortant hpai h5n1 viruses as a candidate poultry vaccine, and we evaluated its immunogenicity and protective efficacy in specific pathogen-free chickens. the 6:2 reassortant h5n1 vaccine strain containing the surface genes of the a/ch ... | 2013 | 23469269 |
| characterization of an avian influenza virus h9n2 strain isolated from a wild bird in southern china. | we isolated an avian influenza virus h9n2 strain from a wild bird in the guangxi province of southern china in 2013 named a/turtledove/guangxi/49b6/2013(h9n2) (gx49b6). we aimed to understand the genetic characters of the gx49b6 strain by analyzing the complete genome sequence. the results showed that our isolated strain has features of low pathogenic avian influenza viruses and viruses that infect humans. the discovery of the complete genome sequence of the gx49b6 strain may be helpful to furth ... | 2014 | 24948768 |
| introgression of ivermectin resistance genes into a susceptible haemonchus contortus strain by multiple backcrossing. | anthelmintic drug resistance in livestock parasites is already widespread and in recent years there has been an increasing level of anthelmintic drug selection pressure applied to parasitic nematode populations in humans leading to concerns regarding the emergence of resistance. however, most parasitic nematodes, particularly those of humans, are difficult experimental subjects making mechanistic studies of drug resistance extremely difficult. the small ruminant parasitic nematode haemonchus con ... | 2012 | 22359506 |
| haemophilus influenzae infection of a prosthetic knee joint in a patient with cll: a vaccine preventable disease. | a 71-year-old man was admitted with a 2-day history of the hot and swollen right knee in december 2012. he had undergone a primary cemented right total knee replacement (tkr) in 2002. he also had a history of chronic lymphatic leukaemia (cll), haemolytic anaemia and splenomegaly, and was in partial remission following chemotherapy in 2008. he underwent arthroscopic washout of right tkr on admission. blood cultures on admission and joint fluid specimen grew haemophilus influenzae (hi). the isolat ... | 2013 | 24252835 |
| characterization of oral helicobacter pylori strain by 4 methods. | the aim of the study was to describe oral helicobacter pylori strain from a child by 4 methods. the strain was positive by immunofluorescence, urea- and caga positive, vaca s1 m2 genotype and resistant to metronidazole and clarithromycin. in conclusion, virulent and antibiotic resistant h. pylori strains can be present in oral cavity from patients with chronic dental and gastroduodenal diseases. | 2013 | 24075629 |
| molecular diagnosis of helicobacter pylori strain by 16s rdna pcr amplification and direct sequencing. | rapid detection of h.pylori strains by pcr-sequencing. | 2011 | 24443707 |
| [detection and significance of gene variation in hepatitis b virus s region]. | to explore the variations of gene s in hepatitis b viruses of hepatitis b patients and provide experimental evidences for the mutation analysis of viral gene. | 2012 | 23134957 |
| re-analysis of unassigned hepatitis c virus (hcv) strain cyhcv025: evidence of a highly divergent lineage within genotype 1. | hcv global sequences have been classified into 7 genotypes, several subtypes and a number of unassigned sequences. our aim was to perform an in depth investigation of the taxonomic relationships of the unclassified cyhcv025 strain by means of phylogenetic analysis. phylogenetic tree reconstructions were performed using ml methods on full-length genomic and partial hcv alignments. phylogenetic analysis of full-length sequences revealed that cyhcv025 clustered close to the root node of genotype 1, ... | 2017 | 28179183 |
| prospective risk analysis of hepatocellular carcinoma in patients with chronic hepatitis c by ultrasound strain elastography. | we have reported about real-time tissue elastography (rte), which displays relative strain by measuring the relative distortion of the tissue, and found this information to be useful for diagnosing liver fibrosis. however, its use in predicting hepatocellular carcinoma has not been reported as yet. here, we investigated rte to predict liver carcinogenesis in patients with chronic hepatitis c virus (hcv) infection. | 2017 | 27750232 |
| impact of type i interferon on the safety and immunogenicity of an experimental live-attenuated herpes simplex virus 1 vaccine in mice. | viral fitness dictates virulence and capacity to evade host immune defenses. understanding the biological underpinnings of such features is essential for rational vaccine development. we have previously shown that the live-attenuated herpes simplex virus 1 (hsv-1) mutant lacking the nuclear localization signal (nls) on the icp0 gene (0δnls) is sensitive to inhibition by interferon beta (ifn-β) in vitro and functions as a highly efficacious experimental vaccine. here, we characterize the host imm ... | 2017 | 28122977 |
| molecular characterisation of varicella zoster virus genotypes in sri lanka. | genotyping of wild type of varicella zoster virus (vzv) in sri lanka would help to distinguish the vzv wild type infection from varicella vaccine associated infections. | 2013 | 24385056 |
| varicella vaccination in japan: necessity of implementing a routine vaccination program. | varicella-zoster virus (vzv) is the causative agent of varicella (chickenpox). it shows extremely high infectivity and is spread by airborne, droplet, and contact transmission. after a person is infected with vzv, the virus remains dormant in the dorsal root ganglia, but can be reactivated under circumstances where specific immunity declines, leading to the development of herpes zoster (shingles). although varicella is a disease that usually resolves after about 1 week, it can cause various comp ... | 2013 | 23483311 |
| artesunate demonstrates in vitro synergism with several antiviral agents against human cytomegalovirus. | human cytomegalovirus (hcmv) infections remain a major problem in immunocompromised patients. three antiviral agents, ganciclovir (gcv), foscarnet (fos) and cidofovir (cdv), are currently approved for the treatment of hcmv infections. they all target the viral dna polymerase and are associated with significant side effects. combinations of novel antiviral compounds acting on different targets such as artesunate (art) with currently approved drugs or eventually letermovir or maribavir (mbv) may r ... | 2016 | 26844400 |
| newly identified transcripts of ul4 and ul5 genes of human cytomegalovirus. | human cytomegalovirus (hcmv) ul4 and ul5 genes are two members of the rl11 gene family. in an earlier study, three ul4 transcripts of about 1.7, 1.5 and 1.4 kb were found in early and late classes after infection by the towne strain by nuclease protection and primer extension analyses. in the present study, two ul4 transcripts (1.5 and 1.7 kb) were found by cdna library screening, northern blot, 3' and 5' race analyses to appear initially in the immediate early phase and one ul4 transcript (1.4 ... | 2015 | 25730208 |
| differences in neutralizing antigenicity between laboratory and clinical isolates of hcov-229e isolated in japan in 2004-2008 depend on the s1 region sequence of the spike protein. | human coronavirus (hcov) is a causative agent of the common cold. although hcov is highly prevalent in the world, studies of the genomic and antigenic details of circulating hcov strains have been limited. in this study, we compared four japanese isolates with the standard hcov-229e strain obtained from atcc (atcc-vr740) by focusing on the spike (s) protein, a major determinant of neutralizing antigen and pathogenicity. the isolates were found to have nucleotide deletions and a number of sequenc ... | 2012 | 22673931 |
| synthesis and anti-hiv activities of suramin conjugates of 3'-fluoro- 2',3'-dideoxythymidine and 3'-azido-2',3'-dideoxythymidine. | conjugates between suramin, a polyanionic naphthalene sulfonate derivative, and nucleoside reverse transcriptase inhibitors (nrtis), 3'-azido-2',3'-dideoxythymidine (azt) and 3'-fluoro-2',3'-dideoxythymidine (flt), were designed to create an antiretroviral with multiple mechanisms of action that could be developed as an anti-hiv topical microbicide candidate. the anti-hiv activity of these conjugates was compared with that of suramin and the corresponding physical mixtures of suramin and nucleos ... | 2012 | 22385137 |
| the establishment and characteristics of cell-adapted ibv strain h120. | avian infectious bronchitis virus is an important pathogen in poultry worldwide. vaccination is the only effective way to prevent and control ibv infection. h120, one of the safest vaccine strains, which has been used worldwide as a primary vaccine, cannot adapt to passaged cells, which severely restricts the quality of the vaccine. based on the reverse genetics of our previous research work, we constructed the recombinant r-h120-beaudette-p65(s) strain by replacing the complete spike gene of h1 ... | 2016 | 27558123 |
| altered pathogenicity of a tl/ch/ldt3/03 genotype infectious bronchitis coronavirus due to natural recombination in the 5'- 17kb region of the genome. | an infectious bronchitis coronavirus, designated as ck/ch/lgx/130530, was isolated from an ibv strain h120-vaccinated chicken in this study. analysis of the s1 gene showed that isolate ck/ch/lgx/130530 was a tl/ch/ldt3/03-like virus, with a nucleotide sequence similarity of 99%. however, a complete genomic sequence analysis showed that ck/ch/lgx/130530 was more closely related to a massachusetts type strain (95% similarity to strain h120) than to the tl/ch/ldt3/03 strain (86%), suggesting that r ... | 2016 | 26616599 |
| [characterization the immunogenicity of recombinant vp2 of infectious bursal disease virus containing n-terminal m2e of avian influenza virus]. | we developed subunit vaccines against h5 or h9 subtype avian influenza viruses (aiv) and infectious bursal disease viruses (ibdv). viral protein 2 (vp2) of ibdv was used as cargo protein to display a 12-amino-acid (aa) immunodominant epitope derived from n-terminal m2 extracelluar domain (nm2e) of h5 or h9 subtype aiv. | 2012 | 22934356 |
| protein clustering and rna phylogenetic reconstruction of the influenza a [corrected] virus ns1 protein allow an update in classification and identification of motif conservation. | the non-structural protein 1 (ns1) of influenza a virus (iav), coded by its third most diverse gene, interacts with multiple molecules within infected cells. ns1 is involved in host immune response regulation and is a potential contributor to the virus host range. early phylogenetic analyses using 50 sequences led to the classification of ns1 gene variants into groups (alleles) a and b. we reanalyzed ns1 diversity using 14,716 complete ns iav sequences, downloaded from public databases, without ... | 2013 | 23667580 |
| polyomavirus jc in the context of immunosuppression: a series of adaptive, dna replication-driven recombination events in the development of progressive multifocal leukoencephalopathy. | polyomavirus jc (jcv) is the etiological agent of progressive multifocal leukoencephalopathy (pml), a demyelinating infection of oligodendrocytes in the brain. pml, a frequently fatal opportunistic infection in aids, has also emerged as a consequence of treatment with several new immunosuppressive therapeutic agents. although nearly 80% of adults are seropositive, jcv attains an ability to infect glial cells in only a minority of people. data suggest that jcv undergoes sequence alterations that ... | 2013 | 23690820 |