| effectiveness of calcium hydroxide-based intracanal medicaments against enterococcus faecalis. | lima rkp, guerreiro-tanomaru jm, faria-júnior nb, tanomaru-filho m. effectiveness of calcium hydroxide-based intracanal medicaments against enterococcus faecalis. international endodontic journal abstract: aim to evaluate the antimicrobial efficacy of calcium hydroxide-based intracanal medicaments against enterococcus faecalis. methodology one hundred and six single-rooted human teeth were inoculated with e. faecalis (atcc 29212) and incubated in an microaerophilic environment for 21 days. tee ... | 2011 | 22077915 |
| antibiotic resistant bacteria/genes dissemination in lacustrine sediments highly increased following cultural eutrophication of lake geneva (switzerland). | this study investigates faecal indicator bacteria (fib), multiple antibiotic resistant (mar), and antibiotic resistance genes (args), of sediment profiles from different parts of lake geneva (switzerland) over the last decades. mars consist to expose culturable escherichia coli (ec) and enterococcus (ent) to mixed five antibiotics including ampicillin, tetracycline, amoxicillin, chloramphenicol and erythromycin. culture-independent is performed to assess the distribution of args responsible for, ... | 2011 | 22051343 |
| A GFP-labeled monitoring tool to quantify conjugative plasmid transfer between Gram-positive and Gram-negative bacteria. | On basis of pIP501 a GFP-tagged monitoring tool was constructed for quantifying plasmid mobilization among Gram-positive bacteria and between Gram-positive Enterococcus faecalis and Gram-negative E. coli. Furthermore, retromobilization of the GFP-tagged monitoring tool was shown from E. faecalis OG1X into the clinical isolate E. faecalis T9. | 2011 | 22138997 |
| antibacterial activity of methyl aminolevulinate photodynamic therapy in the treatment of a cutaneous ulcer. | we describe a 79-year-old female with a chronic venous ulceration infected by staphylococcus aureus and enterococcus faecalis and not responsive to conventional treatments. the patient was treated with methyl-aminolaevulinate photodynamic therapy (mal-pdt). after four weeks the cutaneous swabs become negative and we observed a significant clinical improvement. therefore we suppose that malpdt could represent a valid therapeutic option in the treatment of infected chronic ulcers. | 2011 | 21978711 |
| enterococcus cecorum infections in broiler breeders and their offspring: molecular epidemiology. | increased mortality and problems with lameness were reported in dutch broiler flocks from the year 2008 onwards. therefore, a field inventory, including 10 affected broiler flocks, nine corresponding broiler breeder flocks and five hatcheries, was carried out. the onset of clinical signs (lameness and increased mortality) started at about 2 weeks of age. the flock mortality varied from 3.1 to 8.1% at slaughter. post-mortem lesions of broiler flocks were characterized by the occurrence of pericar ... | 2011 | 22107095 |
| reusable venesection tourniquets: a potential source of hospital transmission of multiresistant organisms. | to determine the prevalence of multiresistant organism (mro) colonisation of reusable venesection tourniquets. | 2011 | 21895597 |
| Bacteriophage lysis of enterococcus host strains: a tool for microbial source tracking? | This paper describes the isolation of Enterococcus host strains, for potential use as simple bacteriophage (phage)-based microbial source tracking (MST) tools. Presumptive Enterococcus host strains were isolated from cattle feces, raw municipal wastewater, agricultural runoff, and waters impacted by farms or wastewater treatment works (WWTW) in southern England, United Kingdom (UK). All enterococcal host strains (n = 390) were first screened for their ability to detect phage in samples of ra ... | 2011 | 22047499 |
| The effects of hyperosmosis or high pH on a dual-species biofilm of Enterococcus faecalis and Pseudomonas aeruginosa: an in vitro study. | To investigate the effect of hyperosmotic hyperosmosis or alkaline stress on a dual-species biofilm of Enterococcus faecalis and Pseudomonas aeruginosa. | 2011 | 21859433 |
| correlation between quantitative polymerase chain reaction and culture-based methods for measuring enterococcus over various temporal scales and three california marine beaches. | several studies have examined how fecal indicator bacteria (fib) measurements compare between quantitative polymerase chain reaction (qpcr) and the culture methods it is intended to replace. here we extend those studies by examining the stability of that relationship within a beach, as affected by time of day and seasonal variations in source. enterococcus spp. were quantified at three southern california beaches in the morning and afternoon using two qpcr assays, membrane filtration, and define ... | 2011 | 22179252 |
| incongruent gelatinase genotype and phenotype in enterococcus faecalis is due to shutting off the ability to respond to the gbap quorum-sensing signal. | the concomitant presence of complete fsr quorum-sensing system and gele-spre operons in enterococcus faecalis is known to be essential for the detection of gelatinase activity. however, there are reports stating the absence of gelatinase activity despite the presence of complete fsr and gele loci. in order to understand this incongruence between genotype and phenotype we sequenced fsr and gele loci of e. faecalis ln68 strain, which was previously found to carry both operons but to lack gelatinas ... | 2011 | 22117005 |
| Time-kill study and synergistic activity of cell-wall inhibitor antibiotics in combination with gentamicin against Enterococcus faecalis and Enterococcus faecium. | The synergy between gentamicin and vancomycin, teicoplanin, ampicillin and linezolid was studied by time-kill method. Two clinical vancomycin resistant enterococci (VRE) and two vancomycin susceptible enterococci (VSE) isolates were used. Different concentrations of antibiotics were combined. Two VSE strains and the control strain exhibited synergism with the combination of gentamicin, vancomycin, teicoplanin, ampicillin and linezolid. Two VRE strains exhibited synergism with the combination of ... | 2011 | 21983323 |
| Chemical Constituents of the Methanolic Extract of Leaves of Leiothrix spiralis Ruhland and Their Antimicrobial Activity. | Chemical fractionation of the methanolic extract of leaves of Leiothrix spiralis Ruhland afforded the flavonoids luteolin-6-C-b-D-glucopyranoside (1), 7-methoxyluteolin-6-C-b-D-glucopyranoside (2), 7-methoxyluteolin-8-C-b-D-glucopyranoside (3), 4'-methoxyluteolin-6-C-b-D-glucopyranoside (4), and 6-hydroxy-7-methoxyluteolin (5), and the xanthones 8-carboxymethyl-1,5,6-trihydroxy-3-methoxyxanthone (6), 8-carboxy-methyl-1,3,5,6-tetrahydroxyxanthone (7). Methanolic extract, fractions, and isolated c ... | 2011 | 22179427 |
| functional and quality characteristics of the red blood cell fraction from biopreserved porcine blood as influenced by high pressure processing. | the effects of high hydrostatic pressure (hhp) processing, at 400mpa for 15min at 20°c, on the microbiological and functional characteristics of the red blood cell (rbc) fraction obtained from porcine blood, previously preserved by means of lactic acid bacteria (lab) was studied. biopreservation was achieved by incubation of inulin-enriched blood inoculated with a lab strain (enterococcus raffinosus ps99) for 72h at 5°c. results showed that incubation of blood with added e. raffinosus followed b ... | 2008 | 22063343 |
| N-Acetylcysteine Inhibits Growth and Eradicates Biofilm of Enterococcus faecalis. | The aims of this study were to evaluate the antibacterial and biofilm eradication efficacies of N-acetylcysteine (NAC) on Enterococcus faecalis. | 2012 | 22152626 |
| In-vitro antimicrobial activity and synergistic/antagonistic effect of interactions between antibiotics and some spice essential oils. | Spices and herbs have been used for many years by different cultures. The aim of the present study is (1) to investigate in-vitro antimicrobial effects of different spices and herbs (5 species: Rosmarinus officinalis (Rosemary), Coriandrum sativum (coriander), Micromeria fruticosa (L.) Druce subsp. Brachycalyx P.H. Davis (White micromeria), Cumium cyminum (cumin), Mentha piperita (Peppermint) against different bacteria and fungi species, and (2) to discuss the in-vitro possible effects between t ... | 2011 | 21888227 |
| Fecal Indicators in Sand, Sand Contact, and Risk of Enteric Illness Among Beachgoers. | BACKGROUND:: Beach sand can harbor fecal indicator organisms and pathogens, but enteric illness risk associated with sand contact remains unclear. METHODS:: In 2007, visitors at 2 recreational marine beaches were asked on the day of their visit about sand contact. Ten to 12 days later, participants answered questions about health symptoms since the visit. F coliphage, Enterococcus, Bacteroidales, fecal Bacteroides, and Clostridium spp. in wet sand were measured using culture and molecular method ... | 2012 | 22157306 |
| Safety evaluation in vitro of Enterococcus durans from Tibetan traditional fermented yak milk. | Despite its ubiquity in fermented dairy products, the safety of lactic acid enterococcal bacteria remains controversial. In this study, five Enterococcus durans strains - A1, A2, B1, B2, and C1 - were isolated from traditional fermented yak milk from Tibet. To evaluate the strains' safety, biogenic amine production, antibiotic resistance and presence of known virulence determinants were investigated. Strain A1 can produce biogenic amines for histamine, spermine, and spermidine (mean values: 8.64 ... | 2011 | 22068487 |
| Microbiological impact of treatment lagoons on the economics of water for reuse in agriculture a case study in Morocco (Settat and Soualem regions). | This study was undertaken to enumerate pathogens: fecal coliforms, Escherichia coli, fecal enterococci and Salmonella in the areas irrigated with treated wastewater. The samples were isolated from Settat (33°00'N, 7°37'W) and Soualem regions (34°26'N, 5°53'W). A total of (n= 48) raw water, (n=48) treated water, (n=71) of vegetables samples irrigated by treated water taken from Waste Water Treatment Plant Settat; A total of (n=24) raw water, (n=24) treated water, (n=97) of vegetables samples irri ... | 2011 | 21955386 |
| Differential effector responses by circulating/blood and tissue/peritoneal neutrophils following burn combined with Enterococcus faecalis infection. | Recently we found that superimposition of Enterococcus faecalis (E. faecalis) infection on burn injury caused an eruption of host mortality not seen with either individual challenge. We hypothesized that the Enterococcus bacteria, and/or factors related to these organisms, aggravate burn-induced modulations in host defense by neutrophils. Our study focuses on alterations in neutrophils' oxidative, proteolytic, and adhesive functions and trans-endothelial migration of neutrophils in burn rats ino ... | 2011 | 22066701 |
| The anti-sigma factor TcdC modulates hypervirulence in an epidemic BI/NAP1/027 clinical isolate of Clostridium difficile. | Nosocomial infections are increasingly being recognised as a major patient safety issue. The modern hospital environment and associated health care practices have provided a niche for the rapid evolution of microbial pathogens that are well adapted to surviving and proliferating in this setting, after which they can infect susceptible patients. This is clearly the case for bacterial pathogens such as Methicillin Resistant Staphylococcus aureus (MRSA) and Vancomycin Resistant Enterococcus (VRE) s ... | 2011 | 22022270 |
| prevalence and antimicrobial susceptibility of enterococcus species isolated from clinical specimens. | multidrug-resistant enterococci are emerging as a leading nosocomial pathogen. knowledge of the antimicrobial resistance profile is essential to formulate treatment guidelines for infection caused by enterococci. a total of 200 enterococcal strains were isolated from various clinical specimens from may 2009 to april 2010 in the department of microbiology, bangladesh institute of health sciences hospital, mirpur, dhaka, bangladesh. they were speciated and an antimicrobial susceptibility pattern w ... | 2011 | 22081191 |
| using the good to beat out the bad: probiotics for eliminating vancomycin-resistant enterococci colonization. | | 2011 | 21989278 |
| method repeatability for measuring enterococcus in southern california beach sands. | a recent study that evaluated 22 methods for enumerating faecal indicator bacteria in sand recommended standardization to a preferred method, but all researchers involved in that study had extensive experience in processing sand samples. the purpose of this study was to evaluate how well the recommended method can be transferred to laboratories without such experience. | 2011 | 21967472 |
| vana-containing enterococcus faecium susceptible to vancomycin and teicoplanin because of major nucleotide deletions in tn1546. | during the course of routine screening for vancomycin-resistant enterococci (vre), we found six enterococcus faecium isolates positive for vana by pcr, but susceptible to vancomycin and teicoplanin by phenotypic testing. the aim of this study was to characterize the genetic composition of the tn1546 vana gene cluster of these isolates. | 2011 | 21926081 |
| evaluation of nd:yag and er:yag irradiation, antibacterial photodynamic therapy and sodium hypochlorite treatment on enterococcus faecalis biofilms. | meire ma, coenye t, nelis hj, de moor rjg. evaluation of nd:yag and er:yag irradiation, antibacterial photodynamic therapy and sodium hypochlorite treatment on enterococcus faecalis biofilms. international endodontic journal. abstract: aim to compare the antimicrobial efficacy of two-high power lasers (nd:yag and er:yag) and two commercial antimicrobial photodynamic therapy (apdt) systems with that of sodium hypochlorite (naocl) action on enterococcus faecalis biofilms grown on dentine discs. m ... | 2012 | 22243483 |
| production of two bacteriocins in various growth conditions produced by gram-positive bacteria isolated from chicken cecum. | lactobacillus plantarum clp29 and enterococcus faecium cle34 isolated from the cecal contents of young broiler chicks were identified based on physiological and biochemical characteristics, and identification was confirmed by 16s rrna sequencing. both bacteria showed a broad range of inhibitory action against bacteria such as salmonella and escherichia coli and produced two peptides, plantaricin clp29 and enterocin cle34. treatment with proteinase k, trypase, or benase resulted in the los ... | 2012 | 22220499 |
| [septic complications after multilocal fractures and multiple traumatic injury]. | the increase of the number of the multiple traumatic injuries is related to social factors, such as: the development of industry, the change of life style and the conditions of work, the manner and the speed of relocation and the biological factors related with the elongation of life time. according to the world health organization data the injuries are one of the main health problems in the world. | 2011 | 22235645 |
| characterization of a vancomycin-resistant enterococcus faecium outbreak caused by 2 genetically different clones at a neonatal intensive care unit. | in july 2010, we identified an outbreak of vancomycin-resistant enterococci (vre) in our 26-bed neonatal intensive care unit. we performed an epidemiological investigation after clinical cultures of 2 neonates were positive for vre. identification, susceptibility testing, and molecular characterization were performed. cultures of 3 surveillance stool samples of inpatients and 5 environmental samples were positive for vre. all isolates were identified as enterococcus faecium containing the vana g ... | 2012 | 22259784 |
| editorial: not so nosocomial anymore: the growing threat of community-acquired clostridium difficile. | clostridium difficile infection is widely accepted to be the leading cause of nosocomial infection-related morbidity and mortality, outpacing both antibiotic-resistant staphylococcus and enterococcus. the existence and prevalence of community-acquired clostridium difficile infection, on the other hand, is much less well appreciated. growing evidence now suggests that community-acquired clostridium difficile infection may account for more than a third of clostridium difficile-associated diarrhea ... | 2012 | 22218031 |
| studies on the biosynthesis of the lipodepsipeptide antibiotic ramoplanin a2. | ramoplanin, a non-ribosomally synthesized peptide antibiotic, is highly effective against several drug-resistant gram-positive bacteria, including vancomycin-resistant enterococcus faecium (vre) and methicillin-resistant staphylococcus aureus (mrsa), two important opportunistic human pathogens. recently, the biosynthetic cluster from the ramoplanin producer actinoplanes atcc 33076 was sequenced, revealing an unusual architecture of fatty acid and non-ribosomal peptide synthetase biosynthetic gen ... | 2012 | 22222159 |
| significance of perioperative infection in survival of patients with ovarian cancer. | perioperative infectious diseases comprise some of the most common causes of surgical mortality in women with ovarian cancer. this study was aimed to evaluate the significance of perioperative infections in survival of patients with ovarian cancer. | 2012 | 22228425 |
| novel vancomycin-resistance transposon, plasmid replicon types, and virulence factors of vancomycin-resistant enterococci in zhejiang, china. | forty-seven vancomycin-resistant enterococcus (vre) strains were isolated from clinical samples in 13 zhejiang hospitals and fecal samples from icu patients in a large teaching hospital in china. no vre isolates were detected in healthy human subjects. cc17 was the main clonal complex in clinical enterococcus faecium isolates but not in isolates from healthy human subjects. novel vancomycin-resistance transposons were detected among vre strains. this is the first report demonstrating insertion o ... | 2012 | 22257297 |
| prevalence of bacterial pathogens in aseer region, kingdom of saudi arabia: emphasis on antimicrobial susceptibility of staphylococcus aureus. | this short study aims to determine the prevalence of various bacterial pathogens causing infections in the aseer regions, and to also assess the distribution of staphylococcus aureus in relation to different body sites as well as their in vitro antimicrobial susceptibility profile. | 2011 | 22216389 |
| lactic acid bacteria from raw milk as potentially beneficial strains to prevent bovine mastitis. | bovine mastitis produces a wide variety of problems in the dairy farm. the treatment of this disease is based on the use of antibiotics which are not always effective. these drugs are also responsible for the presence of residues in the milk and the increase of antibiotic-resistant strains. probiotic products were proposed as a valid alternative to antibiotic therapies and are also useful for the prevention of infectious syndromes. with the aim of designing a probiotic product to prevent bovine ... | 2012 | 22261519 |
| heterocyclic urea derivatives and methods of use thereof (wo2010142978). | a series of ureido derivatives incorporating a highly derivatized quinolone scaffold were prepared by a succession of original steps. the lead molecules of these compounds are the quinolone antibacterials of the nalidixic acid/ciprofloxacin type and they target, similar to these clinically used agents, the dna gyrase/topoisomerase iv of pathogenic bacteria. these new quinolones are claimed to act as efficient antibacterials against a large number of drug-resistant bacteria such as staphylococcus ... | 2012 | 22264034 |
| post-operative wound infection in salvage laryngectomy: does antibiotic prophylaxis have an impact? | salvage laryngectomy carries a high risk of post-operative infection with reported rates of 40-61%. the purpose of this study was to analyse infections in our own patients and review the potential impact of our current antibiotic prophylaxis (ap). a retrospective analysis of infection in 26 consecutive patients between 2000 and 2010 undergoing salvage total laryngectomy (sl) following recurrent laryngeal cancer after failed radiotherapy or chemo-radiation was undertaken. the antibiotic prophylax ... | 2012 | 22274693 |
| characterization of functional, safety, and probiotic properties of enterococcus faecalis ugra10, a new as-48-producer strain. | enterococcus faecalis ugra10, a new as-48-producer strain, has been isolated from a spanish sheep's cheese. the inhibitory substance produced by e. faecalis ugra10 was purified and characterized using matrix-assisted laser desorption ionization-time of flight mass spectrometry, confirming its identity with as-48 enterocin (7.150 da). subsequent genetic analysis showed the existence of the as-48 gene cluster on a plasmid of approximately 70-kb. the ugra10 strain was examined for safety properties ... | 2012 | 22265284 |
| culture heterogeneity in zeta potential is linked to ebp in enterococcus faecalis. | enterococcus faecalis, a commensal of the gastrointestinal tract and an opportunistic pathogen, has the ability to adhere to surfaces and form biofilms. it has been shown earlier that only 10 to 20 percent of a e. faecalis og1rf culture expresses endocarditis and biofilm-associated pili (ebp) which are involved in biofilm formation. another study revealed that e. faecalis clinical isolates, as well as og1rf, are heterogeneous with respect to their apparent zeta potential which was also correlate ... | 2012 | 22267669 |
| environmental dna encoded antibiotics fasamycin a and b inhibit fabf in type ii fatty-acid biosynthesis. | in a recent study of polyketide biosynthetic gene clusters cloned directly from soil, we isolated two antibiotics, fasamycin a and b, which showed activity against methicillin-resistant staphylococcus aureus and vancomycin-resistant enterococcus faecalis. to identify the target of the fasamycins, mutants with elevated fasamycin a mics were selected from a wild type culture of e. faecalis og1rf. next generation sequencing of these mutants, in conjunction with in vitro biochemical assays, showed ... | 2012 | 22224500 |
| genetic characterisation of antibiotic resistance and virulence factors in vana-containing enterococci from cattle, sheep and pigs subsequent to the discontinuation of the use of avoparcin. | the prevalence of vancomycin resistant-enterococci (vre) in faecal samples from cattle, sheep and pigs slaughtered for human consumption was evaluated. enterococci containing the vana gene were detected in 25.3% and 2.7% of the porcine and ovine samples, respectively, and were identified as enterococcus faecium. no vana-containing enterococcal strains were detected in bovine samples. enterococcal strains with intrinsic vancomycin resistance were detected in seven (9.9%) faecal samples from pigs ... | 2012 | 22264646 |
| diversity of enterococcus cecorum from chickens. | enterococcus cecorum is a normal inhabitant of the intestine of birds and other vertebrates. it has recently emerged in canada and other countries as an important cause of arthritis and osteomyelitis in chickens. the objectives of this study were to assess if this emergence was caused by a particular clone of e. cecorum and to assess the antimicrobial susceptibility of this organism. one hundred and thirteen e. cecorum isolates from infections in canadian chickens (cases) and from the ceca of co ... | 2012 | 22266160 |
| inhibition of bacterial carbonic anhydrases and zinc proteases: from orphantargets to innovative new antibiotic drugs. | zinc-containing enzymes, such as carbonic anhydrases (cas) and metalloproteases (mps) play critical functions in bacteria, being involved in various steps of their life cycle, which are important for survival, colonization, acquisition of nutrients for growth and proliferation, facilitation of dissemination, invasion and pathogenicity. the development of resistance to many classes of clinically used antibiotics emphasizes the need of new antibacterial drug targets to be explored. there is a weal ... | 2012 | 22214451 |
| screening of enzymatic activities for the depolymerisation of the marine bacterial exopolysaccharide he800. | the exopolysaccharide (eps) he800 is a marine-derived polysaccharide (from 8 × 10(5) to 1.5 × 10(6) g mol(-1)) produced by vibrio diabolicus and displaying original structural features close to those of glycosaminoglycans. in order to confer new biological activities to the eps he800 or to improve them, structural modifications need to be performed. in particular, depolymerisation is required to generate low-molecular-weight derivatives. to circumvent the use of chemical methods that lack specif ... | 2012 | 22234531 |
| hospital-acquired infections. | health-acquired infection (hai) is defined as a localized or systemic condition resulting from an adverse reaction to the presence of infectious agents or its toxins. this article focuses on hais that are well studied, common, and costly (direct, indirect, and intangible). the hais reviewed are catheter-related bloodstream infection, ventilator-associated pneumonia, surgical site infection, and catheter-associated urinary tract infection. this article excludes discussion of clostridium difficile ... | 2012 | 22269261 |
| development of an intracanal mature enterococcus faecalis biofilm and its susceptibility to some antimicrobial intracanal medications; an in vitro study. | to develop a mature biofilm of enterococcus faecalis inside the root canal system and to test its susceptibility to some antimicrobial medications in vitro. | 2012 | 22229006 |
| overtreatment of enterococcal bacteriuria. | the purposes of this study were to investigate the clinical outcomes of enterococcal bacteriuria and to determine whether current management is adherent to infectious diseases society of america guidelines. | 2012 | 22232145 |
| simultaneous aortic and tricuspid valve endocarditis due to complication of sinus of valsalva rupture. | we experienced a case of ruptured aneurysm of the sinus of valsalva, and this resulted in simultaneous aortic and tricuspid valve endocarditis through a shunt. the echocardiography showed a ruptured sinus of valsalva aneurysm to the right atrium with a shunt. the aortic non-coronary cusp was fibro-thickened with vegetation. vegetations of the septal leaflet and the anterior leaflet of the tricuspid valve were also found. the blood culture grew enterococcus garllinarum. we replaced both tricuspid ... | 2011 | 22263159 |
| chemical compositions of the essential oils of aerial parts of chamaemelum mixtum (l.) alloni. | the chemical compositions of the aerial parts essential oils of chamaemelum mixtum (l.) alloni from corsica and sardinia were investigated employing gas chromatography and gas chromatography/mass spectrometry. the structure of (z)-heptadeca-9,16-dien-7-one, a natural compound not previously described, was elucidated by gas chromatography/mass spectrometry (electron impact and chemical ionization) and one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy. the variation in ch ... | 2012 | 22225420 |
| necrotizing periodontal diseases in hiv-infected brazilian patients: a clinical and microbiologic descriptive study. | objective: nowadays, necrotizing periodontal diseases have a low prevalence; however, a better understanding of the etiopathogenesis of these diseases is necessary for determining more adequate preventive and therapeutic strategies. method and materials: from a pool of 1,232 hiv-infected patients, 15 presented with necrotizing periodontal diseases, which were evaluated by full-mouth periodontal clinical measurements. subgingival biofilm samples were collected from necrotizing lesions of six of t ... | 2012 | 22259811 |
| chemical and biological diversity in fourteen selections of four ocimum species. | biomass, essential oil yield, essential oil composition diversity, and antibacterial and antifungal activities of 14 selections of 4 ocimum species [ocimum basilicum l. (selections: t1-t10), o. gratissimum l. (selections: t11-t12), o. tenuiflorum l.f., syn. o. sanctum l. (selection: t13) and o. kilimandscharicum baker ex. guerke (selection: t14)] were investigated. o. basilicum selections t9 (methyl chavicol: 87.0%) and t10 {(z)- and (e)-methyl cinnamate: 69.1%} produced higher biomass (67.8 and ... | 2011 | 22224293 |
| fine-tuned transcriptional regulation of malate operons in enterococcus faecalis. | in e. faecalis, the mae locus is constituted by two putative divergent operons, maepe and maekr. the first operon encodes a putative h(+)/malate symporter (maep) and a malic enzyme (maee) previously shown to be essential for malate utilization in this bacterium. the maekr operon encodes two putative proteins with significant similarity to two-component systems involved in sensing malate and activating its assimilation in bacteria. our transcriptional and genetic assays showed that maepe and maek ... | 2012 | 22247139 |
| antibacterial effect and cytotoxicity of beta-1,3-1, 4-glucanase from endophytic bacillus subtilis swb8. | we studied the antibiotic activity and selective cytotoxicity of beta-1,3-1,4-glucanase from endophytic bacillus subtilis swb8. | 2011 | 22260051 |
| studies on the effect of an enterococcus faecium probiotic on t cell populations in peripheral blood and intestinal epithelium and on the susceptibility to salmonella during a challenge infection with salmonella typhimurium in piglets. | although enterococcus faecium is used as a probiotic feed supplement in animal production, feeding of the bacterium to piglets resulted in a more severe infection with salmonella typhimurium dt104 during a challenge experiment. to enlighten the mode of action by which e. faecium affected the piglets' health, we investigated the influence of the probiotic bacterium on the development of intestinal and circulating immune cells during a challenge experiment with s. typhimurium dt104. to minimise va ... | 2011 | 22256673 |
| clonal outbreak of st17 multidrug-resistant enterococcus faecium harbouring an inc18-like::tn1546 plasmid in a haemo-oncology ward of a spanish hospital. | objectives: to report a clonal outbreak of st17 vancomycin-resistant enterococcus faecium (vrefm) carrying tn1546 (vana) in a haemo-oncology ward of a tertiary teaching hospital in the south of spain (january-september 2009). patients and methods: twenty-two vrefm strains from 13 patients were characterized by pfge, multiple-locus variable-number tandem-repeat analysis (mlva) and multilocus sequence typing (mlst). genes encoding antibiotic resistance and putative virulence traits and the tn1546 ... | 2012 | 22228676 |
| antimicrobial activity of the investigational pleuromutilin compound bc-3781 tested against gram-positive organisms commonly associated with acute bacterial skin and skin structure infections. | bc-3781 is a novel semi-synthetic pleuromutilin antimicrobial agent which is developed as an intravenous and oral therapy for acute bacterial skin and skin structure infections (absssi) and respiratory tract infections (rti). bc-3781 and comparator agents were tested against 1,893 clinical gram-positive organisms predominantly causing absssi by broth microdilution method. bc-3781 exhibited potent activity against methicillin-resistant staphylococcus aureus (mic(50/90), 0.12/0.25 μg/ml), coagulas ... | 2012 | 22232289 |
| inhibitors of aminoglycoside resistance activated in cells. | the most common mechanism of resistance to aminoglycoside antibiotics entails bacterial expression of drug-metabolizing enzymes, such as the clinically widespread aminoglycoside n-6'-acetyltransferase (aac(6')). aminoglycoside-coa bisubstrates are highly potent aac(6') inhibitors; however, their inability to penetrate cells precludes in vivo studies. some truncated bisubstrates are known to cross cell membranes, yet their activities against aac(6') are in the micromolar range at best. we report ... | 2012 | 22217014 |
| distribution and disinfection of bacterial loadings associated with particulate matter fractions transported in urban wet weather flows. | urban runoff is a resource for reuse water. however, runoff transports indicator and pathogenic organisms which are mobilized from sources of fecal contamination. these organisms are entrained with particulate matter (pm) that can serve as a mobile substrate for these organisms. within a framework of additional treatment for reuse of treated runoff which requires the management of pm inventories in unit operations and drainage systems there is a need to characterize organism distributions on pm ... | 2011 | 22244969 |
| [endocarditis in iceland 2000-2009, a national survey incidence, microbiology and prognosis.] | introduction: the objective of this study was to analyze the incidence, clinical features, microbiology and prognosis of patients with infective endocarditis (ie) in iceland, and to compare the results with a previous study made in iceland 1976-85. material and methods: a retrospective study including all patients diagnosed with ie in iceland 2000-2009. information was obtained from medical records. results: a total of 88 cases (71% men, mean age 59 years) were diagnosed ... | 2012 | 22253080 |
| efficacy of the self-adjusting file system on cleaning and shaping oval canals: a microbiological and microscopic evaluation. | the shaping ability of root canal instruments is determined by a complex interrelationship of parameters such as cross-sectional design and the ability to remove debris and the smear layer. the self-adjusting file (saf) consists of a hollow, flexible instrument in the form of a compressible, thin-walled, pointed cylinder. the aim of this study was to compare the saf with the protaper rotary file system, evaluating debris and smear layer removal and the presence of bacteria by using microbiologic ... | 2012 | 22244642 |
| biodiversity in traditional polish cheese oscypek determined by culture-dependent and -independent approaches. | oscypek is a traditional polish, scalded-smoked cheese, with a protected designation of origin (pdo) status manufactured from raw sheep's milk without starter cultures in the tatra mountains region of poland. this study was undertaken in order to get some insight on the microbiota which develops and evolves during the manufacture and ripening stages of oscypek. to this end, we made use of both culturing and the culture-independent methods of pcr-dgge and pyrosequencing of 16s rrna gene amplicons ... | 2012 | 22247135 |
| influence of enterococcal surface protein (esp) on the transport of enterococcus faecium within saturated quartz sands. | enterococcus was selected by us epa as a gram-positive indicator microorganism for groundwater fecal contamination and its effectiveness is highly dependent on its transport behavior within the groundwater system. in this research, we performed laboratory column transport experiments to investigate the transport of enterococcus faecium of human origin within saturated quartz sands. particularly, we used a wild type strain (e1162) and a mutant (e1162δesp) to examine the influence of enterococcal ... | 2012 | 22243624 |