| monoclonal antibodies to somatostatin receptor of rat brain. | murine monoclonal antibodies (mabs) to the rat brain somatostatin (srif) receptor were produced. sp 2/0 myeloma cells were fused with splenocytes of balb/c mice immunized with the soluble rat brain srif receptor which was partially purified by gel-filtration chromatography. screening by radioligand ([125i-tyr11]srif-14) binding inhibition assay yielded three stable cell lines producing igg1, igm, or iga antibody. autoradiographic study of the polyacrylamide gel electrophoresed under nondenaturin ... | 1992 | 1294456 |
| the effect of dietary safflower phospholipid and soybean phospholipid on plasma and liver lipids in rats fed a hypercholesterolemic diet. | the effect of dietary safflower phospholipid (saf-pl) and soybean phospholipid (soy-pl) on plasma, liver, and fecal lipids in rats fed a hypercholesterolemic diet was compared with that of triglyceride mixture (controls). triglyceride mixture (sp-oil) of safflower oil and palm oil (8:2) contained almost comparable amounts of linoleic acid to safflower phospholipid or soybean phospholipid. concentration of total cholesterol in plasma of rats fed the saf-pl and soy-pl diets were significantly decr ... | 1992 | 1294706 |
| monoclonal antibody sp-21 defines a sialosyl-tn antigen expressed on carcinomas and k562 erythroleukemia cells. | monoclonal antibody (mab) sp-21 resembles the well established mab b72.3 by high affinity binding to peptide linked sialosyl-tn disaccharide, neuac alpha 2-6 galnac alpha, and non-reactivity to the related trisaccharide neuac alpha 2-6 (gal beta 1-3)galnac alpha. although mab sp-21 may be classified, accordingly, into the family of mabs defining the sialosyl-tn antigen, its fine specificity is distinct from the reference antibody b72.3 by binding of mab sp-21 to fetal mucins from human meconium ... | 1992 | 1295441 |
| screening of bacterial strains producing maltotetraose-forming amylase and the conditions for enzyme production. | the authors isolated 1380 bacterial strains from 290 soil samples collected in china and 490 strains were received from other research teams in this institute. by screening 707 strains showed starch-hydrolyzing activity. by further screening and paper chromatographic test, three strains with maltotetraose as the major product were obtained. the maltotetraose was further confirmed by treatment with beta-amylase splitting to maltose and with glucoamylase to glucose. the most promising strain was 5 ... | 1992 | 1295602 |
| inhibition of salmonella ferlac by enterobacteria under in-vitro conditions. | interactions of proteus morganii, proteus mirabilis, proteus sp. klebsiella oxaenae and serratia marcescens isolated from vegetable salads of mass feeding systems with salmonella ferlac (a new subgenus vi of salmonella) isolated from a hostel cook's hands and lizard droppings were recorded following in-vitro nephelometric analysis. nephelometric analysis revealed inhibition of s. ferlac by all the tested isolates from fifth hour of mixed culture interaction. k. oxaenae was the strong inhibitor. | 1992 | 1296949 |
| [candida krusei in genital infections]. | frequency of appearance of candida krusei in human reproductive tract, susceptibility of these microorganisms to drugs and their importance in pathogenesis of mycosis, were investigated. strains c. krusei species constituted 6.7% of the total 1325 strains and 21.8% within other than c. albicans species yeast-like fungi. vaginal mycosis caused by fungi from c. krusei species occurs in presence of lactobacillus sp. and correct values of ph in vaginal contents, however changed leukocytosis in evide ... | 1992 | 1297039 |
| [the role of candida kefyr (c. pseudotropicalis) in reproductive tract infections]. | frequency of appearance of c. kefyr strains, their diagnosis, susceptibility to drugs and importance in pathogenesis of reproductive tract mycoses were investigated. the investigated material consisted of 2717 strains, and there were 67 strains of c. kefyr isolated from vaginal mucous membrane. c. kefyr strains constituted 2.5% of total number of 2717 isolated strains and 8.2% within fungi other than c. albicans species. vaginal mycosis caused by c. kefyr occurs in presence of lactobacillus sp. ... | 1992 | 1297040 |
| close association of peptidergic nerves with lymphocytes in canine and monkey ileal villi. | intimate association of peptidergic nerves with lymphocytes of canine and monkey ileal villi was demonstrated by immunohistochemistry and transmission electron microscopy. a swollen, presumably terminal, portion of nerves containing large cored vesicles and small clear vesicles was in direct contact with a lymphocyte. the apposing membranes of the nerve and lymphocyte were thickened and darkened, being separated by a narrow uniform space. the lymphocyte-associated nerves contained immunoreactivi ... | 1992 | 1297069 |
| [the effect of culture media on bacteriocin production in various strains of bacteria]. | some staphylococcus and enterococcus strains were used to investigate the effect of culture medium on bacteriocin production. staphylococcus cohnii sc7, staphylococcus sp. ztj 151, s. saprophyticus ss 877, enterococcus faecium ef1 and e. faecalis efg2 were isolated from the rumen wall and contents of lambs, calves and fallow deer, enterococcus gallinarum eg10 and e. avium ea12 were isolated from the caecum of japanese quail. the tested bacteria belong to producers with a wide antimicrobial effec ... | 1992 | 1297243 |
| [inhibitory effects of organic acids and salts on selected micromycetes]. | inhibitory effects of two fungistatic preparations (a and b) and of propionic acid were tested in a complete feed mixture for broiler chickens (starter mixture). the water content of this mixture was 25.4%, environmental temperature was 24 degrees c and relative air humidity 90%. propionic acid, which has a high fungistatic effect, served as a standard. the composition of the preparation a was as follows: propionic acid, acetic acid, sorbic acid, citric acid and calcium propionate. the preparati ... | 1992 | 1297244 |
| regional differences of b cell immune responses to (4-hydroxy-3 nitrophenylacetyl) acetic acid (np) antigen in senescent mucosal immune system. | the age-associated primary immune response of b cells from the mesenteric lymph nodes (mln), the bronchial lymph nodes (bln), lamina propria (lp), peyer's patches (pp), and the spleen (sp) of mice following peritoneal or oral immunization with (4-hydroxy-3-nitrophenylacetyl) acetic acid (np) conjugated bovine gamma-globulin (np-bgg) was investigated. the induction of immune responses was assessed in 4-, 11-, and 24-month old individual c57bl/6j male mice by determining the number and isotype of ... | 1992 | 1297408 |
| arthropod parasites of springbok, gemsbok, kudus, giraffes and burchell's and hartmann's zebras in the etosha and hardap nature reserves, namibia. | a total of 48 springbok, 48 gemsbok, 23 kudus and 6 giraffes were examined for ticks and lice, while 9 burchell's zebras and 6 hartmann's mountain zebras were examined only for ticks. springbok and gemsbok were shot in both the etosha national park in the north and the hardap nature reserve in the south of namibia. all the other animals were shot in the etosha national park. a total of 7 ixodid tick species and 8 lice species were recovered. the springbok carried few ticks. the adults of a rhipi ... | 1992 | 1297955 |
| parasites of domestic and wild animals in south africa. xxx. ectoparasites of kudus in the eastern transvaal lowveld and the eastern cape province. | sets of four kudus were shot and examined for arthropod parasites at approximately monthly intervals from april 1981 to march 1983 in the southern part of the kruger national park, eastern transvaal lowveld. these animals harboured 10 ixodid tick species of which boophilus decoloratus followed by amblyomma hebraeum were the most abundant. the seasonal abundances of these ticks and of amblyomma marmoreum, rhipicephalus appendiculatus, rhipicephalus evertsi evertsi and rhipicephalus zambeziensis w ... | 1992 | 1297956 |
| [the cultivation of blastocystis (rhizopoda: lobosea) from hens and ducks]. | the method of cultivation of blastocystis galli from hens and blastocystis sp. from ducks was worked out. blastocystis grow on nutrient medium at ph 7.0 to 7.2 in a wide range of temperatures from 30 to 45 c. optimum temperatures for cultivation are 41 to 42 c. the growth of cultures was obtained on biphase egg medium. solid phase of the medium presents coagulated contents of the hen's egg. liquid phase can be made of henk's solution with the addition of 30% of fresh or lyophilizinic hen serum o ... | 1992 | 1297974 |
| [the restoration of the vocal possibilities for the laryngectomized patient with a tracheoesophageal prosthesis]. | the experience reported concerning vocal rehabilitation employing tracheoesophageal prostheses (tep) in laryngectomized subjects at the university of milan is based upon 149 patients who underwent procedures for total laryngectomy from november 1986 on in nearly all cases (137), a primary procedure was followed in order to insert a blom singer prosthesis. some technical modifications in creating the fistula during the reconstruction phase of laryngectomy reduced the number of postoperative and l ... | 1992 | 1298151 |
| the virulence plasmid does not contribute to growth of salmonella in cultured murine macrophages. | the virulence plasmid, characteristic of many serovars of salmonella sp., and specifically its spv genes, promote intracellular growth of the bacteria in the liver and spleen and are essential for the virulence of these salmonella serovars in the mouse. in an attempt to establish an in vitro model for studying its function, we evaluated its effect on the intracellular growth of the bacteria in macrophages in culture. we used a number of different macrophage-like cell lines (j774-a.1, ic-21 and p ... | 1992 | 1298867 |
| [side chain cleavage of sterols by mycobacterium sp. m12]. | a mutant strain of mycobacterium sp. m12 was obtained. it can convert cholesterol. 3 beta-acetyl-cholesterol, sitosterol and 3 beta-acetyl-sitosterol to 4-androstane-3,17-dione (4-ad) (main product, the yield is 69%, 70%, 26% and 22% respectively) and 1,4-androstadiene-3,17-dione (add) (little amount). optimum conditions for the side chain cleavage of cholesterol and 3 beta-acetyl-cholesterol by mycobacterium sp. m12 were studied. | 1992 | 1299139 |
| de-novo biosynthesis of chlorinated aromatics by the white-rot fungus bjerkandera sp. bos55. formation of 3-chloro-anisaldehyde from glucose. | the white-rot fungus bjerkandera sp. bos55 produced de-novo several aromatic metabolites. besides veratryl alcohol and veratraldehyde, compounds which are known to be involved in the ligninolytic system of several other white-rot fungi, other metabolites were formed. these included anisaldehyde, 3-chloro-anisaldehyde and a yet unknown compound containing two chlorine atoms. additionally gc/ms analysis revealed the production of small amounts of anisyl alcohol and 3-chloro-anisyl alcohol. after 1 ... | 1992 | 1299620 |
| [excitant effect of sodium suberogorgin on isolated rabbit ileum]. | suberogorgin was isolated from gorgoniae suberogorgia sp from south china sea. the isolated rabbit ileum contracted in a concentration-dependent manner by suberogorgin-na (sub) 2.13 mumol.l-1 or higher. sub 213 mumol.l-1 had no effect on the relaxation induced by norepinephrine and isoprenaline 10-100 mumol.l-1 on ileum. the stimulation of sub on ileum was antagonized by atropine 56 mumol.l-1 or indometacin 0.35 mmol.l-1 and was partly blocked by indometacin 0.17 mmol.l-1, but was not affected b ... | 1992 | 1300053 |
| risk of contamination of human and agricultural environment with parasites through reuse of treated municipal wastewater in riyadh, saudi arabia. | two selected sites in the riyadh metropolitan area were surveyed for the presence of human pathogenic parasites in treated municipal wastewater (tmww). a total of 100 samples were collected from both sites at two different seasons, the winter and summer reason. the most common parasites seen were the larvae and adult of strongyloides sp. there were few ascaris lumbricoides ova, some of which were embryonated and possibly infective, while the highest frequency of ascaris ova (100 +/litre) was fou ... | 1992 | 1300348 |
| infection and labor. vii. microbial invasion of the amniotic cavity in spontaneous rupture of membranes at term. | the purpose of this study was to determine the frequency, microbiologic characteristics, and clinical significance of microbial invasion of the amniotic cavity in women with premature rupture of membranes at term. | 1992 | 1301006 |
| collateral reinnervation and expansive regenerative reinnervation by sensory axons into "foreign" denervated skin: an immunohistochemical study in the rat. | immunohistochemistry has been used to study, the capacity of different types of sensory axons in the saphenous nerve to extend into denervated glabrous skin territory after a chronic sciatic nerve lesion. in this study, the extension of the intact or regenerating thin peptidergic and coarse saphenous nerve fibres in adult and neonatal rats was determined. substance p (sp) and calcitonin gene-related peptide (cgrp) antibodies were used as markers for thin axons and neurofilament (nf) antibodies f ... | 1992 | 1301374 |
| [roxithromycin in dental infections]. | roxithromicin is a semisynthetic macrolide antibiotic, with similar antibacterial activity to erythromycin. it is characterized by an excellent pharmacokinetic profile and a good tissue penetration. particularly at the dose of 300 mg per day the dental tissue diffusion is extremely good, and this is important to oppose the oral cavity infections. oral cavity infections can be either odontogenic or non odontogenic. odontogenic infections are typically primary and are caused by commensal bacteria ... | 1992 | 1301492 |
| [alpha-glucosidase inhibitors of microbial origin]. | the data on spreading of inhibitors of alpha-glucosidases with microbial origin are given. physiochemical characteristics of acorbose--a known inhibitor of alpha-glucosidases--and new inhibitor isolated from streptomyces sp. are given in detail. | 1992 | 1302499 |
| free autogenous cartilage grafts to the mandible of rats-histological study. | the present study was designed to histologically evaluate the behavior of free autogenous cartilage grafts to the mandible of rats. a 3-mm segment was removed from the last rib of male adult rats and transplanted fresh to a receptor bed prepared on the mandibular ramus. the results showed that the grafts maintained their vitality up to 120 days and the perichondrium was biologically integrated to the osseous bed. appositional growth of the grafts was found. new bone formation was observed in clo ... | 1992 | 1303116 |
| genetic linkage of the human gene for phenylethanolamine n-methyltransferase (pnmt), the adrenaline-synthesizing enzyme, to dna markers on chromosome 17q21-q22. | we have determined the genetic location of the human gene encoding phenylethanolamine n-methyltransferase (pnmt), the terminal enzyme of the catecholamine pathway catalyzing the synthesis of epinephrine (adrenaline) from norepinephrine. this gene is linked to dna markers on the long arm of chromosome 17, q21-q22, most closely to the dna markers mfd15 (d17s250) (zmax = 15.0, theta = 0.065) and flb17.1 (zmax = 14.6, theta = 0.045). multipoint linkage analysis placed the pnmt locus in the interval ... | 1992 | 1303174 |
| normal hearing in splotch (sp/+), the mouse homologue of waardenburg syndrome type 1. | splotch is considered a model of waardenburg syndrome type i (wsi) because the abnormalities are caused by mutations in homologous genes, pax-3 in mice and pax3 (hup2) in humans. we examined inner ear structure and function in splotch mutants (sp/+) and found no sign of auditory defects, in contrast to the deafness in many wsi individuals. the difference in expression of the genes in the two species may be due to different parts of the gene being mutated, or may result from variations in modifyi ... | 1992 | 1303254 |
| antibacterial in vitro-activity of meropenem against 200 clinical isolates in comparison to 11 selected antibiotics. | the antimicrobial activity of meropenem, a new parenteral carbapenem, was tested in vitro by an agar dilution method against 200 clinical isolates (gram-negative/positive aerobes and anaerobes). meropenem was compared with imipenem, ceftazidime, cefotaxime, piperacillin, ciprofloxacin, gentamicin; and metronidazole, cefoxitin, chloramphenicol, clindamycin, vancomycin when appropriate. meropenem and imipenem exhibited an extended spectrum of activity with low minimal inhibitory concentrations (mi ... | 1992 | 1303691 |
| [immunomodulating action of peptidoglycan of microbial origin]. | the action of a high molecular weight peptidoglycan produced by agrobacter radiobacter sp. on the functional activity parameters in leukocytes and macrophages i. e. chemotaxis and adhesion was studied. it was shown that the peptidoglycan had a stimulating action on the chemotaxis of cells of the peritoneal exudate. a marked stimulating action of the drug on the primary immune response to the tissue antigen of sheep erythrocytes was observed. the peptidoglycan stimulated the antibody titers and d ... | 1992 | 1304124 |
| a quantitative and morphometric study of the transformation of amoeboid microglia into ramified microglia in the developing corpus callosum in rats. | the morphometric and quantitative changes associated with the differentiation of amoeboid microglia into ramified microglial cells in the corpus callosum of rats between 21 d postconception (e21) and 15 d postnatally are described. using lectin labelling, 5 morphological types of labelled cells (r, sp, klp, tlp, ap) based on cell body shape, the configuration of their cytoplasmic processes and their staining intensity, were recognised. round cells (r) and cells with stout processes (sp) were agg ... | 1992 | 1304580 |
| the effect of dietary safflower phospholipid on steroids in gastrointestinal tract of rats fed a hypercholesterolemic diet. | the effect of dietary safflower phospholipid (saf-pl) on the postprandial changes of steroids in the small intestinal and cecal contents was examined in rats fed a hypercholesterolemic diet. the triglyceride mixture (sp-oil) containing a comparable amount of linoleic acid to saf-pl was used as a reference fat source. saf-pl suppressed the elevation of plasma cholesterol levels at all times after meal intake, when compared to sp-oil. the reduction of plasma cholesterol in rats fed the saf-pl diet ... | 1992 | 1304605 |
| louse and mite infestation in domestic animals in northern nigeria. | records of domestic animals brought to the veterinary entomology laboratory for diagnosis of suspected lice and mite infestation over a 10 year period were analysed. from a total of 794 suspected cases, 137 (17.3%) and 247 (31.1%) were positive for lice and mange mites respectively. the most common lice species recorded were linognathus vituli (66.7%) on cattle, l. ovillus (83.3%) on sheep, haematopinus suis (100%) on pigs and menacanthus stramineus (54.5%) on poultry. other lice species recorde ... | 1992 | 1305335 |
| [histopathologic study of the host-parasite relationship: the earthworm-, wild boar-metastrongyle model]. | a histopathological study was conducted on two hosts of metastrongyles (metastrongylus sp.): earthworms and wild boars (sus scrofa linnaeus, 1758). in the earthworm intermediate host there were rare granulomas outside the normal site occupied by larvae (blood sinuses of calciferous glands). given the small number of these reactions, they could not constitute a limiting factor for parasitism in this host. however, the lungs of wild boars contained numerous lesions associated with the presence of ... | 1992 | 1305854 |
| [biochemical and clinical aspects of pulmonary surfactant proteins]. | pulmonary surfactant is a complex mixture of phospholipids and proteins which is synthesized and secreted by alveolar type ii cells. its presence is essential to prevent the collapse of alveoli at the end of expiration. recently, it has been demonstrated that in addition to its reduction of surface tension of alveolar surfaces, pulmonary surfactant exhibits several other functions in the alveolar lining layer, and surfactant proteins are definitely involved in the expression of these functions. ... | 1992 | 1306239 |
| immunohistochemical study of the sympathetic and sensory innervation to the blood vessels of the dog forepaw. | immunohistochemical staining of arteries supplying the dog forepaw showed a dense distribution of nerve fibers which were immunoreactive to tyrosine hydroxylase (th), neuropeptide y (npy), vasoactive intestinal peptide (vip), substance p (sp), and calcitonin gene-related peptide (cgrp) around the vascular walls. the density of each immunoreactive fiber tended to increase in the peripheral branch of the vascular tree. retrograde axonal tracing with fast blue from the artery revealed that these im ... | 1992 | 1306603 |
| changes of fibronectin in the right and left ventricles of rats exposed to chronic normobaric hypoxia. | changes of fibronectin (fn) in the right and left ventricles of adult rats exposed to chronic normobaric hypoxia were observed by a peroxidase immunohistochemical stain technique and analyzed quantitatively by a point counting method. fifty-six rats were randomly divided into control groups of day 0 (immediately prior to the experiment), day 5, day 15, and day 30 and hypoxia groups of day 5, day 15, and day 30. rats of the hypoxia groups were put into a normobaric hypoxia chamber with oxygen con ... | 1992 | 1306605 |
| [new metacercariae of the genus tylodelphys (trematoda, diplostomatidae) in lacustrine populations of galaxias maculatus (teleostei, galaxiidae)]. | metacercariae of three new species of tylodelphys (trematoda, diplostomatidae) were found in the brain of galaxias maculatus (teleostei, galaxiidae), at the meliquina lake, neuquen province, argentina. these metacercariae are morphologically described as tylodelphys argentinus n. sp., tylodelphys barilochensis n. sp. and tylodelphys crubensis n. sp. this is the first time that a stage of genus tylodelphys is mentioned parasitizing galaxias maculatus. metacercariae of t. barilochen sis n. sp. wer ... | 1992 | 1306987 |
| [epidemiological and diagnostic aspects of human cryptosporidiosis in chile]. | cryptosporidium sp. infections have been reported in different chilean localities, both in healthy individuals and immunocompromised patients. among the first, hospitalized and ambulatory children less than 3 years old are the age group more affected reaching frequencies from 3.1 up to 19.3%. in pre-school children, school children and adults the prevalence rates have been 1.1, 0.2 and 0.3% respectively. according to the epidemiological studies carried out, the routine laboratory diagnosis of th ... | 1992 | 1306991 |
| [need for more emphasis on fungal infection in burns]. | we have made quantitative bacterial and fungal culture for 148 biopsy specimens from burn patients. further confirmation was made by frozen section with pas stain in 82 of the specimens. it was discovered that the fungal infections in these burn patients were characterized by early onset with multiple organisms. it was also found that the incidence of fungal infections was higher in severe burns. two rare cases of fungal infection were discovered, one of them was infected by geotrichum sp, and t ... | 1992 | 1307335 |
| [changes in physical, chemical, and microbiological characteristics of brines applied in the salting of mozzarella cheese during the period of utilization]. | variation of the physical, chemical and microbiological characteristics of brines during their utilization for salting mozzarella cheese. forty brine samples used for submersion salting of mozzarella cheese in a dairy industry in the state of s. paulo, brazil, were analysed for the purpose of discovering the variation in the physical, chemical and microbiological characteristics observed over their period of utilization. the mean values preparation up to the 21st day of utilization of ph, sodium ... | 1992 | 1307420 |
| production and regulation of a thermostable protease by pseudomonas sp. b45. | a pseudomonas sp. produced an extracellular thermostable protease which required induction by peptone. growth of the organism and the production of protease was optimum at 30 degrees c. the enzyme was subjected to catabolite repression by glucose. both chloramphenicol and rifamycin completely abolished protease production indicating de novo synthesis of the enzyme. leucine, lysine, histidine and glycine enhanced the protease production considerably and they were the most effective when added dur ... | 1992 | 1307442 |
| [the experimental infection of cebus apella sp. monkeys with trypanosoma cruzi. its clinical, electrocardiographic and anatomicopathological assessment]. | thirty-two monkeys were captured and adapted to laboratory conditions captives isolated. they were submitted to multiple xenodiagnosis which were negative. twelve were infected intraperitoneally with different strains of t. cruzi (1.10(5) to 5.10(6)). twenty were the control group. between on to six years both the control group and the infected monkeys, were submitted to xenodiagnosis, serological testing clinical examination and electrocardiography. the clinical examination and the electrocardi ... | 1992 | 1308068 |
| [neonatal bacterial meningitis: etiological agents in 109 cases during a 10 year period]. | the etiology of purulent meningitis was investigated in 109 newborn infants admitted in a neonatal intensive care unit throughout a ten year period. bacterial pathogens were isolated from the csf in 57 (52.2%) neonates. there was a predominance of gram-negative bacilli isolated in 38 (34.9%) neonates. gram-positive cocci were isolated from csf in only 12 (11.0%) neonates. microorganisms associated with nosocomial septicemia and meningitis in neonates--klebsiella sp, salmonella sp. enterobacter s ... | 1992 | 1308405 |
| catadiscus pomaceae sp. n. (trematoda, paramphistomatidae) from pomacea canaliculata (lamarck, 1801) (prosobranchia, ampullariidae). | catadiscus pomaceae sp. n. from the intestine of the prosobranch mollusc pomacea canaliculata (lamarck, 1801), is described. the host snail was collected from a lenitic biotope belonging to the riachuelo basin (corrientes province, argentina) during 1985-1986. so far the species of the genus catadiscus cohn, 1904 have been recorded in amphibians and reptiles. this is the first instance of a species of that genus parasitizing a mollusc. | 1992 | 1308560 |
| a new host of trypanosoma cruzi from jujuy, argentina: octodontomys gliroides (gervais & d'orbigny, 1844) (rodentia, octodontidae). | to identify wild hosts of trypanosoma cruzi, surveys were conducted in the subandean valleys of jujuy province, argentina, between june 1986 and march 1987. seventy two mammals from 13 different species were examined by xenodiagnosis. fifty two of them were mostly rodents trapped at the localities of maimará, león and tilcara, and the remainder had been kept in captivity at the estación biológica experimental, in jujuy. trypanosoma cruzi infection was detected only in 2 octodontomys gliroides (2 ... | 1992 | 1308567 |
| description of leishmania equatorensis sp. n (kinetoplastida: trypanosomatidae), a new parasite infecting arboreal mammals in ecuador. | characterization is given of a new parasite, leishmania equatorensis sp. n., which was isolated from the viscera of a sloth (choloepus hoffmanni) and a squirrel (sciurus granatensis), captured in humid tropical forest on the pacific coast of ecuador. data based on biological and molecular criteria, as well as numerical zymotaxonomical analysis, indicate that this parasite is a new species of the l. braziliensis complex. l. equatorensis is clearly distinguishable from all other known species with ... | 1992 | 1308568 |
| rapid in vitro detection of hiv-1-specific antibody secretion by cells-culture with virus antigens. | the present report describes an alternative method for in vitro detection of hiv-1-specific antibody secretion in 24h of culture employing as stimulant of peripheral blood mononuclear cells the disrupted inactivated whole virus adsorbed onto microwells in a commercial elisa kit plates. the results obtained from this technique have showed high sensitivity and specificity since it was capable of detecting hiv-1 infection early after birth. there were neither false-positivity nor false-negativity w ... | 1992 | 1308570 |
| [candidiasis in aids patients]. | a total of 35 in patients admitted at emilio ribas hospital--são paulo, brazil, with digestive candidiasis and aids clinical diagnostic were evaluated 10 month later, being 29 male and 6 female; white outnumbering black with age ranged from 30 to 50 years old. agar sabouraud culture and tube germinative tests identified 28 (80%) candida albicans out 35 strains. minimum inhibitory concentration (mic) 50% was against azoles (ketoconazole = 2.2 micrograms/ml; itraconazole = 21.0 micrograms/ml and f ... | 1992 | 1308948 |
| [the prevalence of intestinal parasitoses among the users of the health center of sousas district, campinas, são paulo (1986-1990)]. | the prevalence of intestinal parasites was carried on users of the health center of sousas district, campinas, sp (1986-1990). from 770 observed medical records we verify 114 positive cases (14.8%) for protozoa, helminths and commensals. ascaris lumbricoides (48.2%), giardia lamblia (30.7%), trichuris trichiura (18.4%) and enterobius vermicularis (9.6%) were more prevalent in preschool children. the adults, what are the most numerous class, appeared few parasitised. the other parasites and comme ... | 1992 | 1308950 |
| [the prevalence, infection indices and feeding habits of the triatominae captured in an area of epidemiological surveillance]. | prevalence of triatomines and their infection by trypanosoma cruzi, were studied during 1974-1981, in the administrative region of campinas, são paulo, brazil. this investigation was continued (1982-1986), blood meal tests with bird, marsupial, rodent and human antisera having been added. panstrongylus megistus, predominant in the region, whose winged instars are often found infected by t. cruzi in inhabited houses, displayed and alimentary eclecticism. from a sample of 7,785, 14.78 percent reac ... | 1992 | 1308951 |
| [pulmonary lymph node in acute juvenile paracoccidioidomycosis (a case report)]. | the primary complex like ghon was observed in a child's clinical roentgenographic study. c.s., white, male, 6 years old, was born in curitiba (pr), brazil and living in guaratingueta (sp), brazil, developed "common cold", bimodal diary fever, chills, shake and sweats. dyspnea, cough with general lymphadenopathy. foot and right shoulder arthralgias. six months ago visited a cave, equitation practice, dog and cat contacts and no transfusion, frontal sweats, fever (38.4 degrees c). t.a. was 8/6, ta ... | 1992 | 1308953 |
| [production of monoclonal antibodies against a wild strain of rabies virus]. | production of monoclonal antibodies against a wild strain of rabies virus. cell fusion of sp 2/o, a murine myeloma against a wild strain of rabies virus has originated five monoclonal antibodies (m.a.) specific for virus nucleocapsid , one m.a. specific for virus glycoprotein and one m.a. specific for a viral membrane protein. | 1992 | 1309144 |
| regulation of a cytochrome c2 isoform in wild-type and cytochrome c2 mutant strains of rhodobacter sphaeroides. | in rhodobacter sphaeroides, mutations that suppress the photosynthetic deficiency (spd mutations) of strains lacking cytochrome c2 (cyt c2) cause accumulation of a periplasmic cyt c2 isoform that has been designated isocytochrome c2 (isocyt c2). in this study, a new method for purification of both cyt c2 and isocyt c2 is described that uses periplasmic fluid as a starting material. in addition, antiserum to isocyt c2 has been used to demonstrate that all suppressor mutants contain an isocyt c2 o ... | 1992 | 1309978 |
| lipoxin generation by human megakaryocyte-induced 12-lipoxygenase. | eicosanoid biosynthesis was examined with a human megakaryocytic cell line (dami). megakaryocytes incubated with [1-14c]arachidonic acid and either ionophore a23187 or thrombin generated both thromboxane and 12-hydroxyheptadecatrienoic acid (hhtre). exposure to phorbol myristate acetate (pma) for 1 through 9 days induced differentiation and revealed an increase in the conversion of [1-14c]arachidonate to cyclooxygenase- and lipoxygenase (lo)-derived products. the lo-derived product was identifie ... | 1992 | 1310055 |
| exposure of surfactant protein a to ozone in vitro and in vivo impairs its interactions with alveolar cells. | this study focused on the question of whether exposure of surfactant protein a (sp-a) to ozone affected properties of this protein that may be involved in regulating alveolar type ii cell and alveolar macrophage functions. in vitro exposure of human or canine sp-a to ozone reduced the ability of this protein to inhibit phorbol-ester induced secretion of [3h]phosphatidylcholine by alveolar type ii cells in culture. ozone-exposed human sp-a showed a decreased ability to enhance phagocytosis of her ... | 1992 | 1310226 |
| structural analysis of a novel sialic-acid-containing trisaccharide from rhodobacter capsulatus 37b4 lipopolysaccharide. | sialic-acid-containing lipopolysaccharides from rhodobacter capsulatus 37b4 (s-form lipopolysaccharide), kb-1 (r-type lipopolysaccharide) and sp 18 (deep r-type lipopolysaccharide) were investigated for the linkage and substitution of sialic acids. methylation analysis and behaviour towards acid and enzymic hydrolysis indicated a non-reducing terminal location of sialic acids in the r-type lipopolysaccharide of strain sp 18, whereas an internal, chain-linked location of sialic acids was found in ... | 1992 | 1310942 |
| oxygen-evolving photosystem ii preparation from wild type and photosystem ii mutants of synechocystis sp. pcc 6803. | we present here a simple and rapid method which allows relatively large quantities of oxygen-evolving photosystem ii- (ps-ii-) enriched particles to be obtained from wild-type and mutants of the cyanobacterium synechocystis 6803. this method is based on that of burnap et al. [burnap, r., koike, h., sotiropoulou, g., sherman, l. a., & inoue, y. (1989) photosynth. res. 22, 123-130] but is modified so that the whole preparation, from cells to ps-ii particles, is achieved in 10 h and involves only o ... | 1992 | 1311205 |
| expression of surfactant associated protein-a and clara cell 10 kilodalton mrna in neoplastic and non-neoplastic human lung tissue as detected by in situ hybridization. | two markers for the progenitor cells of peripheral airways and their tumors are the 10 kilodalton (kd) clara cell protein and the major surfactant associated protein-a (sp-a). we used the rna-rna in situ hybridization technique to study expression of the genes encoding these proteins at the cellular level in 19 pairs of non-neoplastic and neoplastic tissues from resected human lungs. our results show that in non-neoplastic lung tissue, the clara 10 kd protein gene was expressed in nonciliated ce ... | 1992 | 1311398 |
| the regulation of connective tissue metabolism by vasoactive intestinal polypeptide. | immunohistochemical studies have confirmed the innervation of bone with neuropeptidergic neurons containing vasoactive intestinal polypeptide (vip), substance p (sp) and calcitonin gene-related peptide (cgrp). in this study, we report effects of vip on connective tissue cell metabolism. vip stimulated pge2 production in human articular chondrocytes, human osteoblast-like cells and human synovial cells, however, stromelysin production was unaffected. vip also stimulated camp production in human o ... | 1992 | 1311458 |
| involvement of a chlorobenzoate-catabolic transposon, tn5271, in community adaptation to chlorobiphenyl, chloroaniline, and 2,4-dichlorophenoxyacetic acid in a freshwater ecosystem. | a chlorobenzoate-catabolic transposon (tn5271) was introduced on a conjugative plasmid (pbrc60) in the natural host, alcaligenes sp. strain br60, into lake water and sediment flowthrough microcosms. experimental microcosms were exposed to micromolar levels of 3-chlorobenzoate, 4-chloroaniline, 2,4-dichlorophenoxyacetate, or 3-chlorobiphenyl. the populations of the host, br60, and organisms carrying tn5271 were monitored over a 100-day period by use of selective plate counts and the most-probable ... | 1992 | 1311543 |
| cloning and nucleotide sequence of the gene coding for citrate synthase from a thermotolerant bacillus sp. | the structural gene coding for citrate synthase from the gram-positive soil isolate bacillus sp. strain c4 (atcc 55182) capable of secreting acetic acid at ph 5.0 to 7.0 in the presence of dolime has been cloned from a genomic library by complementation of an escherichia coli auxotrophic mutant lacking citrate synthase. the nucleotide sequence of the entire 3.1-kb hindiii fragment has been determined, and one major open reading frame was found coding for citrate synthase (ctsa). citrate synthase ... | 1992 | 1311544 |
| the activation of camp-dependent protein kinase is directly linked to the stimulation of bone resorption by parathyroid hormone. | the present study was performed to characterize the direct involvement of camp in the stimulation of bone resorption by parathyroid hormone (pth), using sp-camps and rp-camps, which were the direct agonist and antagonist in the activation of camp-dependent protein kinase (pka), respectively. bone resorbing activity was estimated as the number of pits formed on the dentine slice and total area of pits per slice in bone marrow cells derived from 2 week-old mice. dibutyryl camp (dbcamp)(10(-4)m) an ... | 1992 | 1311571 |
| vasoactive intestinal peptide stimulates immunoglobulin production and growth of human b cells. | the effect of vasoactive intestinal peptide (vip) on human lymphoblastoid b cell lines and tonsil b cells was studied. vip increased immunoglobulin production and proliferation by lymphoblastoid b cell line, gm-1056, in a dose-dependent manner. as little as 10(-12) m of vip was effective, and higher concentrations of vip induced an approximately five-fold increase in iga production. moreover, this enhancement was blocked by vip antagonist. similarly, vip enhanced igm and igg production by other ... | 1992 | 1311995 |
| differential effects of progesterone treatment on the oxytocin-induced prostaglandin f2 alpha response and the levels of endometrial oxytocin receptors in ovariectomized ewes. | the oxytocin-induced uterine prostaglandin (pg) f2 alpha response and the levels of endometrial oxytocin receptors were measured in ovariectomized ewes after they had been given steroid pretreatment (sp) with progesterone and estrogen to induce estrus (day of expected estrus = day 0) and had subsequently been treated with progesterone over days 1-12 and/or pgf2 alpha over days 10-12 postestrus. the uterine pgf2 alpha response was measured after an i.v. injection of 10 iu oxytocin on days 13 and ... | 1992 | 1312363 |
| ws-7338, new endothelin receptor antagonists isolated from streptomyces sp. no. 7338. i. taxonomy, fermentation, isolation, physico-chemical properties and biological activities. | ws-7338 a, b, c and d, novel endothelin receptor antagonists, have been isolated from fermentation broth of streptomyces sp. no. 7338. these antagonists were purified from the culture mycelium by extraction with acetone, followed by carbon column chromatography and hplc. among them, ws-7338 b showed good activity in an endothelin receptor binding assay with an ic50 of 2.7 x 10(-7) m. | 1992 | 1312521 |
| ws-7338, new endothelin receptor antagonists isolated from streptomyces sp. no. 7338. ii. biological characterization and pharmacological characterization of ws-7338 b. | ws-7338, produced by streptomyces sp. no. 7338, was found to be a competitive and specific antagonist against et-1 receptors in in vitro studies and ws-7338 b is also active in vivo. furthermore, ws-7338 b was a specific antagonist for vascular et-1 receptors (eta receptors) and significantly prevented the accumulation of intracellular inositol 1,4,5-triphosphate (ip3) in endothelin treated rat aorta tissues. | 1992 | 1312522 |
| concomitant cryptosporidia, coronavirus and parvovirus infection in a raccoon (procyon lotor). | a juvenile raccoon (procyon lotor) was found moribund near fort collins, colorado (usa). upon examination, the raccoon was dehydrated, had a mucopurulent oculonasal discharge and diarrhea, and was euthanized. postmortem examination revealed emaciation, severe fibrinous gastroenteritis and a small, firm liver. histopathological findings included blunting of villi, infiltration of lamina propria with neutrophils and plasma cells, and mild bronchopneumonia. cryptosporidium sp. was demonstrated on i ... | 1992 | 1312645 |
| characterization of the promoter region of the murine fibroblast growth factor receptor 1 gene. | to obtain some clue for the regulatory mechanism by which fibroblast growth factor (fgf) receptor 1 (fgfr 1) gene is expressed, we have cloned the promoter region of this gene from genomic library of mouse fgf-responsive cell lines. the genomic clone isolated here includes the fgfr 1 gene from position -868 to +697 relative to transcription initiation site. sequence analysis reveals the presence of various consensus sequences for the binding sites of transcriptional factors such as sp 1, gcf, oc ... | 1992 | 1312839 |
| nmr and epr studies on a monoheme cytochrome c550 isolated from bacillus halodenitrificans. | a c-type monoheme ferricytochrome c550 (9.6 kda) was isolated from cells of bacillus halodenitrificans sp.nov., grown anaerobically as a denitrifier. the visible absorption spectrum indicates the presence of a band at 695 nm characteristic of heme-methionine coordination. the midpoint redox potential was determined at several ph values by visible spectroscopy. the redox potential at ph 7.6 is 138 mv. when studied by 1h-nmr spectroscopy as a function of ph, the spectrum shows a ph dependence with ... | 1992 | 1312933 |
| specific binding of surfactant apoprotein sp-a to rat alveolar macrophages. | surfactant protein a (sp-a) influences the function of alveolar macrophages in vitro. in this study the characteristics of the binding of 125i-labeled sp-a to rat alveolar macrophages has been investigated. the binding of sp-a to alveolar macrophages at 4 degrees c was saturable with half-maximal binding at a sp-a concentration of 4 micrograms/ml. bound sp-a was rapidly displaced by an excess of unlabeled sp-a. the binding of labeled sp-a to the alveolar macrophages was blocked in a dose-depende ... | 1992 | 1314502 |
| the direct involvement of camp-dependent protein kinase in the regulation of collagen synthesis by parathyroid hormone (pth) and pth-related peptide in osteoblast-like osteosarcoma cells (umr-106). | the present study was performed to characterize the direct involvement of camp-dependent protein kinase (pka) in the regulation of collagen synthesis by parathyroid hormone (pth) and pth-related peptide (pthrp) in osteoblastic osteosarcoma cells, umr-106. sp-camps (10(-4)m), a direct activator of pka, as well as dibutyryl camp (dbcamp, 10(-4)m) significantly inhibited collagen synthesis. human (h) pth-(1-34) (10(-7)m) and hpthrp (10(-7) m) inhibited collagen synthesis to the same degree. althoug ... | 1992 | 1314599 |
| unhydrolyzable analogues of adenosine 3':5'-monophosphate demonstrating growth inhibition and differentiation in human cancer cells. | a set of adenosine 3':5'-monophosphate (camp) analogues that combine exocyclic sulfur substitutions in the equatorial (rp) or the axial (sp) position of the cyclophosphate ring with modifications in the adenine base of camp were tested for their effect on the growth of hl-60 human promyelocytic leukemia cells and ls-174t human colon carcinoma cells. both diasteromeres of the phosphorothioate derivatives were growth inhibitory, exhibiting a concentration inhibiting 50% of cell proliferation of 3- ... | 1992 | 1314695 |
| gene transfer in magnetic bacteria: transposon mutagenesis and cloning of genomic dna fragments required for magnetosome synthesis. | broad-host-range incp and incq plasmids have been transferred to the aerobic magnetic bacterium aquaspirillum sp. strain amb-1. conjugal matings with escherichia coli s17-1 allowed high-frequency transfer of the rk2 derivative prk415 (4.5 x 10(-3) transconjugant per recipient cell) and the rsf1010 derivative pkt230 (3.0 x 10(-3) transconjugant per recipient). these plasmids successfully formed autonomous replicons in transconjugants and could be isolated and transformed back into e. coli, illust ... | 1992 | 1314800 |
| m-phase-specific histone h1 kinase in fish oocytes. purification, components and biochemical properties. | we demonstrate, for the first time in fish, that a ca(2+)-independent and cyclic-nucleotide-independent histone h1 kinase activity oscillates according to the cell cycle of the oocyte, peaking at the first and the second meiotic metaphase with a transient drop between them. the kinase, m-phase-specific histone h1 kinase (m-h1k), was purified from mature carp oocytes by using two exogenous substrates for assaying its activity: histone h1 and a synthetic peptide (sp peptide, kkaakspkkakk) containi ... | 1992 | 1315270 |
| novel aerobic 2-aminobenzoate metabolism. purification and characterization of 2-aminobenzoate-coa ligase, localisation of the gene on a 8-kbp plasmid, and cloning and sequencing of the gene from a denitrifying pseudomonas sp. | a new pathway for the aerobic metabolism of 2-aminobenzoate which proceeds via 2-aminobenzoyl-coa has recently been revealed in a pseudomonas strain kb 740-. the enzyme catalyzing the first step, the formation of the coenzyme a (coa) thioester of 2-aminobenzoate, is 2-aminobenzoate-coa ligase. it was purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source and characterized. it is rather specific for 2-aminobenzoate, but activates also benzoate and f ... | 1992 | 1315272 |
| purification and characterization of membrane-bound endoglycoceramidase from corynebacterium sp. | endoglycoceramidase catalyzes the hydrolysis of the linkage between oligosaccharides and ceramides of various glycosphingolipids. we found that a bacterial strain corynebacterium sp., isolated from soil, produced endoglycoceramidase both intracellularly and extracellularly. the intracellular enzyme bound to the cell membrane was solubilized with 1% triton x-100 and purified to homogeneity about 170-fold with 60% recovery. the molecular mass of the enzyme was approximately 65 kda. the enzyme is m ... | 1992 | 1315273 |
| [cytomegalovirus associated diseases of the heart]. | latent cytomegalovirus (cmv) infection is not uncommon in the juvenile and adult population. the full blown disease is mostly restricted to immunosuppressed and immunodeficient patients, but may also occur in healthy individuals. the acute cmv-myocarditis often takes a mild course with only transient changes of left ventricular hemodynamics or a pericardial effusion as assessed by echocardiography. in our patient population it was characterized by the presence of anti-interfibrillary antibodies. ... | 1992 | 1315712 |
| selective inhibition by dactinomycin of nanc sensory bronchoconstriction and [125i]nka binding due to nk-2 receptor antagonism. | in the present study, dactinomycin (10(-5) m) inhibited the non-adrenergic, non-cholinergic bronchoconstriction upon antidromic vagal nerve stimulation (1 hz for 1 min) in the isolated perfused guinea-pig lung by 84%. the release of calcitonin gene-related peptide was unchanged, however, suggesting a postjunctional action. dactinomycin (10(-5), 5 x 10(-5) m) also reduced non-adrenergic non-cholinergic bronchial contractions (maximally by 75%) induced by electrical field stimulation or capsaicin, ... | 1992 | 1316711 |
| in vitro, candida albicans releases the immune modulator adenosine and a second, high-molecular weight agent that blocks neutrophil killing. | we previously described a lyophilized supernatant from germinated candida albicans that blocks human neutrophil (pmn) o2- production and degranulation stimulated by several pmn agonists but does not block stimulation by pma. in studies to further characterize this candida hyphal inhibitory product (chip), we noted several physicochemical parallels with the purine nucleoside adenosine (ado). a sephadex g-10 semipurified fraction of chip had an absorption peak near 260 nm, an apparent m.w. of less ... | 1992 | 1316920 |
| alterations in the water intake caused by central inhibition of angiotensin-converting enzyme in the rat. | in the present study we investigated the effects of central (i.c.v.) and subcutaneous (s.c.) injections of a 2 micrograms dose of lisinopryl, an inhibitor of angiotensin i(angi)-converting enzyme (ce), on water intake. i.c.v. but not s.c. injection of lisinopryl abolished drinking in response to s.c. isoprenaline (100 micrograms/kg) and significantly reduced drinking in response to 24 h water deprivation or s.c. polyethylene glycol (30% w/v, 10 ml/kg). lisinopryl had no effect on water intake in ... | 1992 | 1317024 |
| activation of g proteins by (rp) and (sp) diastereomers of guanosine 5'-[beta-thio]triphosphate in hamster fibroblasts. differential stereospecificity of gi, gs and gp. | the effects of guanosine 5'-[beta-thio]triphosphate (gtp beta[s]) on g proteins have been examined in chinese hamster lung fibroblasts (ccl39 line) permeabilized with alpha-toxin from staphylococcus aureus. although much less effective than guanosine 5'-[gamma-thio]triphosphate (gtp gamma[s]), both (rp) and (sp) diastereomers of gtp beta[s] were found to activate three g protein-mediated pathways: inhibition of forskolin-stimulated adenylate cyclase (mediated by gi), potentiation of receptor-med ... | 1992 | 1318029 |
| isolation and characterization of peptides which act on rat platelets, from a pheochromocytoma. | an increase in the cellular concentration of camp leads to the inhibition of platelet aggregation. we have been investigating endogenous peptides which inhibit platelet function, using an assay which detects increase in platelet camp. compared with the human adrenal medulla, a pheochromocytoma (pc) contained abundant peptides that elevate platelet camp. about 90% of the activity was found in the sp-iii fraction which contained strongly basic peptides. from the sp-iii fraction, peptides p-1, p-2 ... | 1992 | 1318039 |
| pulmonary cestodiasis in a cynomolgus monkey (macaca fascicularis). | cestodiasis in primates has been noted historically to occur quite frequently, although tissue damage and clinical signs may or may not be apparent. larval cestodes, such as hydatid cysts or cysticercus cysts, are known to cause extensive tissue damage, while other larval cestodes, such as tetrathyridia, cause minimal damage to the host. this case history concerns an apparently healthy cynomolgus monkey that was part of a surgical study. during the study, all parameters were normal. the monkey d ... | 1992 | 1318447 |
| site-directed conversion of a cysteine to aspartate leads to the assembly of a [3fe-4s] cluster in psac of photosystem i. the photoreduction of fa is independent of fb. | the terminal electron acceptors fa and fb exist as two [4fe-4s] clusters located on the 8.9-kda psac protein in photosystem i. we have used site-directed mutagenesis to produce a complementary pair of mutant psac proteins in which specific cysteine ligands to the [4fe-4s] clusters were changed to aspartic acid residues. the mutant proteins, denoted c14d and c51d, were overproduced in escherichia coli; the iron-sulfur clusters were inserted in vitro; and the reconstituted proteins were rebound to ... | 1992 | 1318744 |
| phospholipids chiral at phosphorus. stereochemical mechanism for the formation of inositol 1-phosphate catalyzed by phosphatidylinositol-specific phospholipase c. | the phosphatidylinositol-specific phospholipase c (pi-plc) from mammalian sources catalyzes the simultaneous formation of both inositol 1,2-cyclic phosphate (icp) and inositol 1-phosphate (ip). it has not been established whether the two products are formed in sequential or parallel reactions, even though the latter has been favored in previous reports. this problem was investigated by using a stereochemical approach. diastereomers of 1,2-dipalmitoyl-sn-glycero-3-(1d- [16o,17o]phosphoinositol) ( ... | 1992 | 1318746 |
| surfactant protein c: hormonal control of sp-c mrna levels in vitro. | we have studied hormonal regulation of the surfactant protein c (sp-c) in fetal 18-dah rat lung explants. sp-c mrna was detected in northern blots with a specific rat sp-c cdna probe and quantified by densitometry. treatment of the explants with dexamethasone resulted in a dose-dependent increase of the sp-c mrna level. transcriptional assays have shown that the regulation of sp-c mrna by dexamethasone involves a transcriptional step. administration of the camp analogues, 8-bromoadenosine 3',5'- ... | 1992 | 1319687 |
| defense reaction elicited by microinjection of kainic acid into the medial hypothalamus of the rat: antagonism by a gabaa receptor agonist. | electrical stimulation of either the midbrain central gray or the medial hypothalamus induces a defense reaction in the rat, characterized mainly by increased locomotion, rearing, and leaping. however, microinjection of the excitatory amino acid glutamate was effective only in the former region. because excitatory amino acids do not depolarize axons of passage, it was suggested that the hypothalamus is devoid of soma/dendrites of neurons commanding the defense reaction. in the present study, we ... | 1992 | 1319704 |
| second messenger signaling in the regulation of cytosolic ph and dna synthesis by parathyroid hormone (pth) and pth-related peptide in osteoblastic osteosarcoma cells: role of na+/h+ exchange. | the present study was performed to investigate the regulation of cytosolic ph (phi) and dna synthesis by parathyroid hormone(pth) and pth-related peptide (pthrp) in osteoblasts, using osteoblastic osteosarcoma cells, umr-106 which possessed pth-responsive dual signal transduction systems (camp-dependent protein kinase (pka) and calcium/protein kinase c [ca/pkc]) and amiloride-inhibitable na+/h+ exchange system. both human (h)pth-(1-34) and hpthrp-(1-34) caused a progressive decrease in phi and t ... | 1992 | 1320038 |
| isolation and sequence analysis of a gene encoding a basic cytochrome c-553 from the cyanobacterium anabaena sp. pcc 7937. | | 1992 | 1320427 |
| transcriptional regulation of the plastocyanin and cytochrome c553 genes from the cyanobacterium anabaena species pcc 7937. | the effect of copper on the levels of plastocyanin (pc) and cytochrome c553 (cyt-c)-specific transcripts from anabaena sp. pcc 7937 was investigated. the addition of copper resulted in a marked increase in pc mrna levels, and a decrease in cyt c mrna levels. thus the functional exchange between pc and cyt c seems to be regulated at the mrna level. the copper-dependent increase in pc and decrease in cyt c mrna levels was abolished when chloramphenicol was added to the cells. this suggests that de ... | 1992 | 1320727 |
| adp receptor-induced activation of guanine-nucleotide-binding proteins in human platelet membranes. | adp receptor-regulated binding of the labeled gtp analog, guanosine 5'-o-(3-[35s]thiotriphosphate) ([35s]gtp[gamma s]), to guanine-nucleotide-binding proteins (g proteins) was studied in human platelet membranes. the potent adp receptor agonist, 2-methyl-thio-adenosine 5'-diphosphate (2mesadp), a non-hydrolyzable analog of adp, increased the binding of [35s]gtp[gamma s] without apparent lag phase. under optimal conditions, i.e. in the presence of gdp (1-10 microm), 2mesadp increased the binding ... | 1992 | 1321045 |
| purulent meningitis due to aspergillosis in a patient with systemic lupus erythematosus. | we report a 39-year-old female patient with systemic lupus erythematosus under immunosuppressive therapy who developed persistent neutrophilic meningitis, for which no infectious agent could be identified. intensifying the immunosuppressive therapy induced a short amelioration of the clinical picture. at autopsy, basal meningitis was found to be due to aspergillus sp. | 1992 | 1321697 |
| subgingival temperature (iii). relation to microbial counts. | the present investigation examined the relationship of selected bacterial species and subgingival temperature. 35 subjects were measured at 6 sites per tooth for clinical parameters and subgingival temperature. measurements were repeated for 21 subjects at 2 month intervals providing a total of 66 subjects visits. at each visit, subgingival plaque samples were taken from the mesial aspect of each tooth and anaerobically dispersed, diluted and plated on non-selective media. after anaerobic incuba ... | 1992 | 1321846 |
| highly potent and selective heptapeptide antagonists of the neurokinin nk-2 receptor. | incorporation of d-pro9 into substance p related peptides is known to enhance neurokinin nk-2 receptor agonist potency and selectivity with respect to other neurokinin receptors. we now report that replacement of d-trp9 by d-pro9 in the nonselective neurokinin antagonist [arg5,d-trp7,9, nle11]-sp(5-11) gave a partial agonist with nk-2 receptor selectivity. further incorporation of pro10 provided the weak but selective nk-2 antagonist arg-ala-d-trp-phe-d-pro-pro-nle-nh2 (compound 4; nk-2 pkb = 5. ... | 1992 | 1321907 |
| differential effects of cytokines on long-term mitogenic and secretory responses of fetal rat pancreatic beta-cells. | it has been proposed that certain cytokines secreted by islet-infiltrating leukocytes may be involved in the pathogenesis of insulin-dependent diabetes mellitus by participation in beta-cell destruction. in the present study, the impact of various cytokines on replication and long-term insulin secretion by pancreatic beta-cells was investigated. to this end, fetal rat pancreatic islets containing a high fraction of beta-cells were exposed in culture for 1-3 days to interleukin-1 beta (il-1 beta) ... | 1992 | 1322036 |
| evidence from directed mutagenesis that aspartate 170 of the d1 polypeptide influences the assembly and/or stability of the manganese cluster in the photosynthetic water-splitting complex. | to identify amino acid residues that influence the assembly or stability of the manganese cluster in photosystem ii, we have generated site-directed mutations in the d1 polypeptide of the cyanobacterium, synechocystis sp. pcc 6803. indirect evidence has suggested that the d1 polypeptide provides some of the ligands that are required for metal binding. mutations at position 170 of d1 were selected for characterization, since an aspartate to asparagine mutation (dn170d1) at this position completel ... | 1992 | 1322168 |
| protection against tabun toxicity in mice by prophylaxis with an enzyme hydrolyzing organophosphate esters. | we demonstrate here the correlation between protection afforded by pretreatment alone with parathion hydrolase purified from pseudomonas sp. against tabun toxicity in mice and the kinetic parameters which are assumed to determine the in vivo detoxification of tabun by the same enzyme. results show that 15 and 22 micrograms of parathion hydrolase per animal conferred a protective ratio of 3.94 and 5.65 respectively, against tabun toxicity, without post-exposure treatment. | 1992 | 1322669 |
| the effect of epithelium removal on leukotriene e4-induced histamine hyperresponsiveness in guinea-pig tracheal smooth muscle. | 1. removal of the epithelium resulted in a threefold increase in guinea-pig tracheal sensitivity to histamine without increasing the maximal response. 2. preincubation of epithelially-denuded guinea-pig tracheal smooth muscle with leukotriene e4 (lte4) in vitro increased the subsequent maximal response of the tissues to histamine. the sensitivity of the tissues to histamine was unaffected by lte4 pretreatment. 3. pretreatment of the epithelially-denuded tissues with the lte4-analogue, 20-cooh lt ... | 1992 | 1324073 |
| enhancement of the circulating antibody secreting cell response in human diarrhea by a human lactobacillus strain. | human lactobacillus sp strain gg (lactobacillus gg) administered during acute rotavirus diarrhea has been shown to promote clinical recovery. to elucidate the immune mechanisms behind such a favorable outcome, the elispot (solid phase enzyme-linked immunospot) assay of ig- and specific antibody-secreting cells among circulating lymphocytes was used, giving indirect evidence of the immunologic events in the gut. after rehydration, 39 children with acute rotavirus diarrhea, mean age 16 (sd 6) mo, ... | 1992 | 1324462 |
| isolation and characterization of is1165, an insertion sequence of leuconostoc mesenteroides subsp. cremoris and other lactic acid bacteria. | we have cloned and characterized an insertion sequence from leuconostoc mesenteroides subsp. cremoris strain db1165. this element, designated is1165, is 1553 bp, has imperfect inverted repeat ends, contains an open reading frame of 1236 bp, and is not related to any previously described insertion sequence. the copy number of is1165 varies from 4 to 13 in l. mesenteroides subsp. cremoris strains allowing genetic fingerprinting of strains based on location and number of bands on hybridization. is1 ... | 1992 | 1325060 |