| cellular immunity to tumor-associated antigens of transplantable mammary tumors of c3h/hen mice. | | 1979 | 83909 |
| induction of high frequencies of endoreduplication in mammalian cell cultures with 33258 hoechst and rubidazone. | cells of the mouse l strain and the chinese hamster cho line were treated with 33258 hoechst, rubidazone, and a combination of these. recovering cell populations following the drug removal exhibited a high frequency (20--50%) of metaphases with diplochromosomes (endoreduplication), especially in the combination treatment series (up to more than 70% in the l strain). such a procedure should be useful in probing the mechanisms of the endoreduplication process. | 1979 | 83930 |
| immunogenetic analysis of "natural killer" activity in the mouse. | | 1979 | 83960 |
| reactions of chicken antibodies with immunoglobulins of mouse serum and t cells. | | 1978 | 83961 |
| serological identification of neoantigens on mouse fibroblasts which have undergone "spontaneous" malignant alteration in vitro. | st-l1 is a cell line established from lung explants from a normal st/a mouse. the st-l1 cells have undergone spontaneous malignant alteration in vitro. the cells were rejected after inoculation into syngeneic immunocompetent hosts, and a syngeneic humoral immuneresponse against the st-l1 cells has been detected. the specificity of this humoral response was investigated. the syngeneic response to st-l1 was characterized by indirect immunofluorescence tests and by immunoprecipitation of radiolabel ... | 1979 | 83967 |
| biochemical relationship between murine immune interferon and a killer cell helper factor. | concanavalin a and alloantigen-stimulated mouse spleen cells release into the supernatant at least two distinct antigen-nonspecific factors that enhance the generation of cytotoxic cells in vitro. as previously reported, analysis of the supernatant by ammonium sulfate fractionation, gel filtration, hydroxylapatite chromatography, and hydrophobic chromatographic showed one of the two killer cell helper factors (khf) to be associated with thymocyte mitogenic factor. this report demonstrates the se ... | 1979 | 84015 |
| generation of cytolytic t lymphocytes in vitro. x. induction of primary and secondary ctl responses by the mitogen sodium periodate. | short-term (15 min) sodium periodate (naio4) treatment of mouse spleen cells previously primed in vivo or in vitro against alloantigens induced the formation of secondary (2 degree) cytolytic t lymphocytes (ctl) specific for the priming antigens. ctl formation was readily demonstrable within 24 hr after treatment. this early ctl response occurred equally well in the presence or absence of cytosine arabinoside (ara c), indicating that naio4 could induce ctl independently of dna synthesis. forty-e ... | 1979 | 84021 |
| f1 hybrid anti-parental h-2k cell-mediated lympholysis. i. stimulator and target determinants controlled by the h-2k region. | h-2k-heterozygous f1 hybrid mouse spleen cells cultured with irradiated h-2k-homozygous stimulator cells generated specific anti-parent cytolytic effectors. the parental antigenic determinants recognized by responder cells during induction (afferent arm) and by effector cells during cytolysis (efferent arm) were coded for, or regulated by, the h-2k-hh3 region of the mhc, according to recombinant analysis. there were no detectable influences by other linked or unlinked genes on the phenotypic exp ... | 1979 | 84028 |
| [3h]uridine uptake by target monolayers as a terminal label in an in vitro cell-mediated cytotoxicity assay. | a terminal labelling method is described for measuring cell-mediated cytotoxicity based on the ability of surviving target cells to incorporate [3h]uridine into their rna precursor pools. parameters of the system were examined using whole and damaged embryonic mouse fibroblast monolayers. this assay is less laborious than direct cell counting and gives increased sensitivity at low target to effector cell ratios. the labelling time is short and, unlike similar techniques, it allows target cell mo ... | 1979 | 84034 |
| attempts to reproduce amyotrophic lateral sclerosis in laboratory animals by inoculation of schu virus isolated from a patient with apparent amyotrophic lateral sclerosis. | a virus isolated from the csf of a patient who had amyotrophic lateral sclerosis for 7 years, and prolonged pleocytosis in the csf, was adapted to suckling mouse brain by subsequent serial blind passages. this schu virus belongs to the tick-borne encephalitis complex of the genus flavivirus (togaviridae). suckling mouse brain homogenate of the 13th passage was used for transmission experiments in various species of laboratory animals. golden hamsters infected subcutaneously fell ill after a numb ... | 1979 | 84063 |
| differences in cell surface antigens of tumor metastases and those of the local tumor. | tests were made to determine whether cell surface tumor antigens of metastases differed from the tumor antigens of the cell population of the local tumor growth. c57bl/6 mouse spleen lymphocytes sensitized against monolayers of the local growth of the 3ll lewis lung carcinoma (l-3ll) in the presence of syngeneic serum generated lymphocytes that were cytotoxic to l-3ll but significantly less cytotoxic to target cells derived from lung metastases (m-3ll). lymphocytes sensitized against m-3ll were ... | 1979 | 84091 |
| serologically defined, unique surface antigen on a mouse mammary adenocarcinoma. | syngeneic a.sw mice were immunized with sublethal viable cells of a spontaneous mammary adenocarcinoma s3w. the serum was tested by complement-dependent cytotoxicity against in vitro-cultured s3w cells and a spectrum of controls. s3w cells were found to react with at least four different kinds of antibodies in the serum. one antigen was present on several leukemia cell lines. a second cross-reactive antigen was detected on polyoma virus-induced tumors. a third was demonstrated on other mammary c ... | 1979 | 84092 |
| natural polyclonality of spontaneous akr leukemia and its consequences for so-called specific immunotherapy. | young female akr mice made leukemic by iv inoculation of 10(3) spontaneous akr thymoma cells were treated with repeated injections of irradiated cells from the same tumor. treatment began 1 day after injection of the viable cells. the cytotoxicity of sera and lymphoid cells from healthy mice immunized with lymphoma cells from either treated or nontreated mice with leukemia grafts revealed that the tumor cells could be subdivided into four distinct antigenic types. one type (clone a) accounted fo ... | 1979 | 84093 |
| co-suppression of virus production and erythroid differentiation in friend erythroleukemic cell x non-erythroid mouse cell hybrids. | | 1979 | 84391 |
| the expression of histocompatibility-2 antigens on hemopoietic stem cells. | antisera directed against histocompatibility-2 antigens of the mouse suppress the formation of spleen colonies by pluripotent hemopoietic stem cells. of antisera which are specific for subregions of the h-2 complex, only those which contain anti-h-2k or anti-h-2d activity are effective. specific anti-ia sera do not react with the stem cell. if the titer of the cfu-s suppressive effect of the antisera is compared to their toxicity to spleen lymphocytes, it can be concluded that cfu-s express anti ... | 1979 | 84415 |
| study of the glycolipid composition of the milk fat-globule membrane and the mouse mammary tumour virus prepared from the milk of infected swiss mice [proceedings]. | | 1978 | 84579 |
| isolation of single-stranded dna sequences coding for cellular and viral rna in mouse cells transformed by simian virus 40 [proceedings]. | | 1978 | 84624 |
| inactivation in vivo of mouse liver glycogen synthase by phenylephrine, vasopressin and glucagon: effect of anaesthesia [proceedings]. | | 1978 | 84648 |
| fibroblast interferon in man is coded by two loci on separate chromosomes. | we have examined viral and poly(rl):poly(rc) induction of interferon synthesis in several human, mouse and chinese hamster cell lines, and in hybrids derived from the fusion of such cells. we observed species and cell-type differences in inducer effectiveness and in the kinetics of interferon production. in some cases, parental characteristics are preserved in somatic cell hybrids, and in other cases, the expression of the donor phenotype is modulated by the epigenetic state of the recipient cel ... | 1979 | 84714 |
| a defined subgenomic fragment of in vitro synthesized moloney sarcoma virus dna can induce cell transformation upon transfection. | the longest dna molecules synthesized by endogenous reverse transcription in detergent-permeabilized moloney murine sarcoma virus (mo-msv) virions (clone g8-124) are double-stranded dna molecules of 5,8 kilobase pairs (kbp). this dna species has been purified by sedimentation of total in vitro synthesized mo-msv dna through neutral sucrose gradients. a physical map of the positions of the cleavage sites for a series of restriction endonucleases has been derived for this 5.8 kbp dna. mo-msv dna s ... | 1979 | 84715 |
| sgc (small granule chromaffin) cells in the mouse adrenal medulla: light and electron microscopic identification using semi-thin and ultra-thin sections. | adrenal glands of the mouse, fixed either in glutaraldehyde followed by osmium tetroxide or in a mixture of potassium dichromate and glutaraldehyde, and embedded in epon 812, were investigated by light and electron microscopy. an argentaffin reaction was applied to semi-thin sections for light microscopy and to ultra-thin sections for electron microscopy. since the mature secretory granules in the small granule chromaffin (sgc) cell were argentaffin and were mainly located along the cell membran ... | 1978 | 84757 |
| inhibition of condensation of human y chromosome by the fluorochrome hoechst 33258 in a mouse-human cell hybrid. | the fluorochrome hoechst 33258 which binds preferentially to a-t base pairs, drastically inhibits the condensation of a-t-rich centromeric heterochromatin regions in mouse cell lines. the condensation of all other regions of these chromosomes is also inhibited to some extent. the human y chromosome contains a large heterochromatic region, which is also rich in a-t base pairs. this chromosome is not affected by hoechst 33258 in human leukocyte cell cultures. on the other hand, condensation of the ... | 1979 | 84782 |
| localization of nors in chromosomes of mouse cell lines by a combined 33258-hoechst and ag-staining technique. | | 1979 | 84805 |
| murine model for immunoprophylaxis of cytomegalovirus infection. i. efficacy of immunization. | murine cytomegalovirus was utilized as a model for human cytomegalovirus, which had no experimental animal, to study immunoprophylaxis of the cytomegalovirus infections. (1) murine cytomegalovirus (mcmv) serially propagated in mouse embryonic fibroblasts had lost pathogenicity for weanling mice including neonatally thymectomized mice. (2) the cell culture-adapted mcmv was effective as a "live, attenuated virus vaccine" against challenge by virulent, mouse-passaged mcmv. (3) the immunization via ... | 1978 | 85239 |
| quantitation of enzymes in tissue sections by estimation of hydrolytic activity and antigenic determinants. | the method and instrumentation for the analysis of enzyme activity and of fluorescent antibody of antigen binding is described. it is based on the microscopic photometry of stain intensities per area of functional units. thus it was shown that the concentration of aminopeptidase (ec 3.4.2.1) per area in procimal tubule of kidney from pig and mouse is constant. in contrast to the pig the tubules from the outer medulla of the mouse contained twice as much enzyme as those from the cortex. a non con ... | 1976 | 85312 |
| studies on the cytophilic properties of human beta2-microglobulin. ii. the role of histocompatibility antigens. | the mechanism by which exogenously added beta2m binds to lymphoid cells has been explored. in the mouse it has been shown that beta2m remains associated with plasma membrane macromolecules following solubilization with np-40 and that approximately 25-30% of the binding could be accounted for by binding to h-2 antigens. no binding to mouse immunoglobulin or ia antigens could be detected. the sites for binding of the remainder of the cell-bound beta2m were not determined. whereas normal human lymp ... | 1979 | 85331 |
| differences in mouse ovarian cells as distinguished by horseradish peroxidase labelling. | the extracellular space of mouse ovarian follicles and stroma contained horseradish peroxidase (hrp) reaction product one minute after intravenous injection of the tracer. in addition to pinocytotic uptake of the tracer, non-vesicular staining with hrp reaction product, heretofore unrecognized, was noted in a variety of ovarian cell-types. this diffuse staining was correlated with changes in cellular morphology suggestive of degeneration. these findings are discussed in relation to the compositi ... | 1979 | 85420 |
| antiproliferative effects of 9-beta-d-arabinofuranosyladenine 5'-monophosphate and related compounds in combination with adenosine deaminase inhibitors against mouse leukemia l1210/c2 cells in culture. | the antiproliferative activity of 9-beta-d-arabinofuranosyladenine 5'-monophosphate against a cultured line of mouse leukemia cells (l1210/c2) was enhanced by addition of either 2'-deoxycoformycin or erythro-9-(2-hydroxy-3-nonyl)adenine. the activity of 9-beta-d-arabinofuranosyladenine 5'-monophosphate, alone or in combination with either of the two inhibitors of adenosine deaminase, was comparable to that of 9-beta-d-arabinofuranosyladenine (ara-a), apparently reflecting the rapid conversion of ... | 1979 | 85484 |
| heterogeneity in cell-associated transformation-sensitive proteins antigenically related to fibronectin. | antiserum to purified fibronectin has been used to investigate transformation-associated heterogeneity in surface components antigenically related to fibronectin. examination of extracts from surface-labeled rat cells by gradient gel electrophoresis revealed in "normal" cells the presence of two species with approximate molecular weights of 250,000 and 230,000, which were decreased in wild-type transformed cells. reaction with antifibronectin serum revealed the selective precipitation of the two ... | 1979 | 85490 |
| alterations in the response to erythropoietin and rna-dependent dna polymerase activity in mouse spleen cells infected with friend leukemia virus. | | 1979 | 85492 |
| an in vitro model for induction of immunological unresponsiveness to turkey gamma-globulin in primed mouse spleen cells. i. the secondary in vitro response and induction of unresponsiveness. | | 1979 | 85495 |
| an in vitro model for induction of immunologic unresponsiveness to turkey gamma-globulin in primed mouse spleen cells, ii. antigen-specific suppressor cells. | | 1979 | 85496 |
| pattern of activity of nucleolus organizers during spermatogenesis in mammals as analyzed by silver-staining. | silver-staining in the nuclei and chromosomes of spermatogenesis of four species of mammals (man, mus musculus, rattus norvegicus, and cavia cobaya) was investigated qualitatively and quantitatively. these species show a very similar pattern of activity of the nucleolus organizer regions (nors) during the various stages of spermatogenesis. silver precipitates are detectable in growing spermatogonia and up until the pachytene stage of meiotic prophase. during the meiotic metaphases i and ii and d ... | 1979 | 85513 |
| differential changes of auditory nerve and brain stem short latency evoked potentials in the laboratory mouse. | subcollicular, volume-conducted auditory evoked potentials were obtained from the c57bl/6 laboratory mouse. barbiturate-induced, whole body hypothermia was associated with a latency increase; between 37 and 31 degrees c, these values were 0.28, 0.48, 0.88 and 0.88 msec for pi-iv, respectively. the amplitude of the auditory nerve potential elicited by a 100 db click doubled in amplitude between 16 and 42 days post partum. a progressive latency decrease, from pi to piv, was observed during this ag ... | 1979 | 85541 |
| internal synthesis of p23,30 by several lymphoid malignancies. | the aim of this study was to prove the internal synthesis of p23,30 antigen (hla-d related determinant) on human leukemias and lymphomas on which it has been detected with complement-dependent cytotoxic assays. murine ia antigens similar to p23,30 antigen are found on many subsets of cells in the mouse (b lymphocytes, macrophages, allogeneically activated t lymphocytes) and on intercellularly transferred immunoregulatory molecules, which may be adsorbed to other cells. the question exists whethe ... | 1979 | 85554 |
| murine type-c virus expression in human x mouse hybrid cells [proceedings]. | | 1979 | 85560 |
| cultivation of fresh and frozen mouse bone marrow. - application of methyl cellulose. | the culture of cells of both fresh and frozen mouse bone marrow on methyl cellulose (mc) was approached. we used 1.5%-concentration of mc and proved the stem cells of fresh and frozen mouse bone marrow to proliferate and form haemopoietic colonies on mc. we established that the freezing process did not significantly decrease the proliferative capacity of cfu-c stem cells. | 1978 | 85584 |
| serological analysis of species specificity in the high mobility group chromosomal proteins. | the non-histone chromosomal protein of the high mobility group (hmg-1) present in mouse liver was purified to homogeneity. antibodies against this protein as well as pure hmg-1 derived from calf thymus and hmg-e purified from duck erythrocytes were elicited in rabbits. the interaction between the antibodies and the immunogens was measured by passive hemoagglutination and by quantitative microcomplement fixation. quantitative microcomplement fixation assays revealed that the immunological distanc ... | 1979 | 85628 |
| tissue distribution of human alpha1-microglobulin. | human alpha(1)-microglobulin was isolated from the urine of patients with tubular proteinuria, and its molecular weight was established by sodium dodecyl sulfate-polyacrylamide gel electrophoresis at 33,000 daltons. the carbohydrate content was 21.7%. anti-alpha(1)-microglobulin serum was prepared and observed to react monospecifically in gel diffusion to purified alpha(1)-microglobulin, as well as to normal human serum and urine. sera from the domestic chicken, mouse, rat, rabbit, dog, calf, co ... | 1979 | 85635 |
| cell-surface antigens associated with recombinant mink cell focus-inducing murine leukemia viruses. | distinct type-specific antigens were detected on cells infected with cloned mink cell focus-inducing (mcf) murine leukemia viruses by means of cell surface immunofluorescence absorption assays with rabbit antisera raised against naturally-occurring akr mcf viruses. the mcf type-specific antibodies were present in high titer and not absorbable by cells infected with ecotropic, xenotropic, or wild mouse amphotropic murine leukemia viruses, or combinations of ecotropic and xenotropic viruses. three ... | 1979 | 85682 |
| the induction of cell-mediated immunity and tolerance with protein antigens coupled to syngeneic lymphoid cells. | a mouse model of cell-mediated immunity (cmi) and tolerance to protein antigens horse gamma globulin (hogg) and cytochrome (cyt c) was investigated. a reliable cmi response as measured in vivo by ear swelling or by an in vitro t-cell proliferation assay could be induced by one of two methods: (a) sensitization by antigen-complete freund's adjuvant in the base of the tail, or (b) sensitization by s.c. injection of antigen coupled to syngeneic lymphoid cells. the in vivo response exhibited charact ... | 1979 | 85683 |
| the distribution of myelin basic protein in subcellular fractions of developing jimpy mouse brain. | | 1979 | 85694 |
| [perichordal necroses. contribution to the understanding of the spinal column morphogenesis]. | in 12--15 days old rat embryos and 12 days old mouse embryos vitally stained by acridin orange, a perichordal necrosis appears, showing metameric intensifications at the level of the dense sclerotomites. similar aspects were detected in serially sectioned rabbit and human embryos (6--7 mm c.r. length). the authors correlate the necrotic zones mentioned with the inductive functions of the notochord. | 1978 | 86154 |
| transplantation of human prostate neoplasia into the nude mouse. | cells derived from human prostates have been successfully transplanted from tissue culture and from primary surgical specimens into nude mice. techniques of transplantation and results are discussed. | 1978 | 86170 |
| simultaneous production of q and r bands after staining with chromomycin a3 or olivomycin. | human and mouse chromosomes, stained with either chromomycin a3 or olivomycin, which bind preferentially to g - c-rich dna (where g is guanosine and c is cytosine), exhibit a q or a reverse banding pattern, depending on the wavelength used for excitation. the two complementary banding patterns can be observed in the same metaphase simply by changing the combination of excitation filters. these data suggest, therefore, that in addition to base composition, other factors are involved in the produc ... | 1979 | 86207 |
| detecting enteropathogenic strains of escherichia coli of porcine origin. 2. relationship between o and k antigens and the production of enterotoxin in strains isolated from the piglet after weaning. | the production of enterotoxin by biological tests (yl adrenal cells and the suckling mouse) has been examined in 96 strains of escherichia coli, isolated from sick piglets after weaning. there is a good correlation between the presence of the capsular antigen k88 and that of the thermolabile fraction (lt) of the enterotoxin (69.2 per cent of the enteropathogenic strains studied). however, the presence of the thermostable fraction (st) of the enterotoxin of strains which, according to their serol ... | 1978 | 86320 |
| passive sensitization of mice and rats with ige antibodies. | the strain-dependent variation in susceptibility to passive sensitization of the skin and peritoneal mast cells with homologous ige antibodies was observed in inbred strains of mice. strain 129 showed low susceptibility to passive sensitization and high efficiency of normal serum in blocking mouse reagin-induced pca in rats. balb/c and c3h/a strains showed higher susceptibility to passive sensitization and the efficiency of normal serum in blocking rat pca lower than strain 129. the serum factor ... | 1978 | 86339 |
| evidence for the presence of an antitumor factor in serum of normal animals. | we have previously reported finding a factor with antitumor activity (tnf, tumor necrosis factor) in extracts of serum from normal mice. the possibility that tnf exists in the blood of normal animals of other species was explored. horse, mouse, dog, human, sheep, calf, rat and shark serums were fractionated with (nh4)2so4 and filtered through s-200 sephacryl gel. proteins of molecular weight 90,000 to 180,000 were pooled, concentrated and dialyzed. tnf, determined by l-cell assay in vitro and me ... | 1979 | 86384 |
| differences in the expression of histocompatibility antigens on mouse lymphocytes and tumor cells: immunochemical studies. | immunochemical studies have shown that labeled, detergent-solubilized extracts of sl2 (h-2d) lymphoma cells contain components reactive with several anti-h-2 alloantisera of restricted specificity. anti-h-2k and anti-h-2ja as well as anti-h-2d sera precipitated labeled polypeptides of a molecular weight similar to that of h-2 heavy chains. in addition, all antisera tested precipitated a component of 70000 daltons molecular weight, which is antigenically related to gp 69/71 of friend murine leuke ... | 1979 | 86449 |
| normal immunosuppressive protein inhibition of human and murine lymphoblastoid cell line proliferation. | normal immunosuppressive protein, prepared from human plasma by deae-cellulose chromatography, inhibits dna synthesis in human cell lines of lymphocytes of both t and b origin. it also inhibits [3h]thymidine incorporation in mouse cell lines. normal immunosuppressive protein was able to inhibit the proliferation of these cells, although they were already transformed and had a high rate of dna synthesis. on the other hand, it does not inhibit myeloid cells or fibroblasts. | 1979 | 86450 |
| liver-specific and shared cell membrane antigens. studies by light- and electron microscopy. | liver-specific and shared saline-insoluble cell surface antigens were localized by immunofluorescence as well as by light- and electron microscopic immunoenzyme techniques. antisera against purified mouse liver cell membranes were surface membrane but not organ-specific. variable quantities of shared antigens were present in endoderm- and mesoderm-derived organs but not in ectodermal nerve tissue. species crossreactivity was observed for the rat. repeated absorption produced liver-specific antis ... | 1978 | 86503 |
| alpha-foetoprotein:immunoreactivity of the major oestrogen-binding component in mouse amniotic fluid. | competitive protein binding studies, using oestrone as the inhibitor, demonstrated that mouse amniotic fluid contains a high affinity oestradiol-binding component precipitable by monospecific rabbit anti-alpha-foetoprotein (afp) in a double-antibody radio-immunoassay. on disc gel radio-electrophoresis the oestrogen-binding entity migrated as an alpha-1 protein which was immunoreactive to anti-afp serum. immunoprecipitation of protein-bound [3h]-oestradiol from solution was proportional to anti-a ... | 1979 | 86511 |
| protease inhibitors reduce mitogen induced lymphocyte stimulation. | proteolytic events might play a role during lymphocyte activation. mouse spleen cells were therefore stimulated in serum-free cultures by pha, cona, lps and dextran sulphate and the effect of various added protease inhibitors on [3h]-thymidine incorporation investigated. both soybean inhibitor and trasylol inhibited the response of the cells to all mitogens. the other inhibitors (antipain, leupeptin, ovomucoid, alpha-1 trypsin, alpha-2 macroglobulin) had little or no effect. the marked inhibitor ... | 1979 | 86514 |
| a new virion precipitation test for oncovirus envelope antigens which detects common antigenic determinants in mammalian type-c viruses and mason-pfizer monkey virus. | a method for the study of oncovirus envelope antigens was developed, bases on the precipitation of intact virions by a double antibody technique. the amount of precipitated virus was then measured as reverse transcriptase activity. the method was designated the virion precipitation test (vpt). it has been used for titration of antibodies to envelope antigens of oncoviruses. the study of envelop antigens of 11 different oncoviruses permitted their differentiation into the following groups: (1) mu ... | 1979 | 86521 |
| cell-mediated reactivity to antigens shared by moloney-virus-induced lymphomas (lstra) and certain 3-methylcholanthrene-induced mouse sarcomas. | spleen cells (sc) both from balb/c mice whose primary moloney sarcoma virus (msv)-induced sarcomas had spontaneously regressed and from normal, untreated balb/c mice, were co-cultivated for 5 days with mitomycin-c-treated lstra cells; lstra is a balb/c moloney lymphoma which shares cell surface antigens with msv-indiced sarcomas. these sc, referred to as cmr and cu cells, respectively, were shown to be cytotoxic to lstra cells in 3 h 51cr-release assays; cmr cells showed, in most cases, the grea ... | 1979 | 86527 |
| interplexiform cell of the mouse retina: a golgi demonstration. | a neuron of the inner nuclear layer (inl) with some processes extending to the outer plexiform layer (opl) and others to the inner plexiform layer (ipl) was discovered near the posterior pole of the mouse retina. the neuron's location and appearance are similar to the interplexiform cells of several other species. the relatively recent recognition of this cell type along with its characteristic of infrequent staining by the golgi technique make the extent of its distribution among species uncert ... | 1979 | 86528 |
| the development of the hippocampus and dentate gyrus in normal and reeler mice. | the histogenesis, the time of origin and the pattern of migration of the cells in the hippocampus and dentate gyrus, have been studied in normal and reeler mice. the earliest indication of a defect in the reeler hippocampus is seen on the fifteenth embryonic day (e15) which is at least 24 hours after the first indication of a defect in the neocortex. it is not until e18, that the dentate gyrus shows signs of its incipient abnormality. it appears then, that in both the hippocampus and the dentate ... | 1979 | 86549 |
| flow cytometry of mammalian sperm: progress in dna and morphology measurement. | variability in dna content and head shape of mammalian sperm are potentially useful markers for flow cytometric monitoring of genetic damage in spermatogenic cells. the high refractive index and extreme flatness of the sperm heads produce an optical effect which interferes with dna measurements in flow cytometers which have dye excitation and fluorescence light collection normal to the axis of flow. orientation of sperm in flow controls this effect and results in coefficients of variation of 2.5 ... | 1979 | 86565 |
| quantitation of lymphocyte response to pha by flow cytofluorometry. iii. heterogeneity of induction period. | early events in phytohaemagglutinin (pha) stimulation of mouse splenocytes have been quantitated by using flow cytometry and supravital staining with acridine orange (ao). increasing percentages of single cells with increased metachromatic (red) ao staining were demonstrated in cultures stimulated by pha for up to 24 hr. these differences in staining could be eliminated by fixation with 1:1 ethanol/acetone before staining. stimulated cells showed an increase in nonspecific esterase activity as m ... | 1979 | 86571 |
| prostacyclin: a solution to some problems of extracorporeal circulation. experiments in greyhounds. | in cardiopulmonary-bypass experiments in greyhounds the effects of adding prostacyclin, prostacyclin plus heparin, and heparin alone to the extracorporeal circulation were compared. with heparin alone platelet count and function were reduced, the pressure differential across the arterial filters rose, platelet deposits were found on the arterial filters, and plasma-fibrinogen levels fell. plasma from these dogs was toxic to fetal-mouse hearts in culture. with prostacyclin alone, fibrinogen level ... | 1979 | 86720 |
| [immunological study of rsv-induced mouse brain tumor. i. induction of tumor specific cytotoxic t cells (author's transl)]. | | 1979 | 86973 |
| in vitro transformation of mouse bone marrow cells by the polycythemic strain of friend leukemia virus. | strains of friend leukemia virus (flv) that are associated with polycythemia contain the defective spleen focus-forming virus (sffv). to determine whether the transforming ability of flv was affected by the presence of this second agent, dba/2j mouse bone marrow cells were infected in vitro. criteria for transformation were the establishment of permanent lines, growth on semisolid agarose, and the production of tumors at the site of inoculation in syngeneic hosts. two lines of immature hematopoi ... | 1979 | 86991 |
| the plasma cell differentiation antigen pc.1 is absent in ch3/tif and present in c3h/hej. | (c3h/tifxdba/2)f1 mice, immunized with viable balb/c plasmacytoma mopc315 cells, produce antibodies directed against a cell-surface antigen. the strain and tissue distribution of this antigen was identical to that of the plasma cell differentiation alloantigen pc.1. the antigen is absent in the mouse strain c3h/tif but is present in the closely related substrain c3h/hej. this is the third difference between surface structures of the b-cell lineage of c3h/tif and c3h/hej mice. | 1979 | 87010 |
| phenotypes of 'null' lymphoid cells in human blood. | f(ab')2 antibody fragments of heteroantisera directed against p28,33 (or 'ia-like') antigen, t-cell antigen(s) (t), myeloid antigen (m), and immunoglobulin have been used along with rosetting techniques to analyse the antigenic heterogeneity of 'null' or 'unclassified' lymphoid cells purified 'negatively' from human blood by e rosette depletion of t cells and b removal on an anti-immunoglobulin immunoabsorbent. the results confirm that considerable heterogeneity exists within the 'null' cell pop ... | 1979 | 87011 |
| the cellular infiltrate in contact hypersensitivity to picryl chloride in the mouse. | in the present work, the contact hypersensitivity skin test reaction to picryl chloride in cba mice was examined. the test was performed by applying the contact allergen to the ear skin and making a series of histological analyses up to 24 hours after challenge. an increment in ear thickness, measured with an engineer's micrometer 24 hours after challenge, was obvious in a group of sensitized mice when compared with a nonsensitized control group, and the difference was found to be highly signifi ... | 1979 | 87073 |
| human serum and epithelial spread in tissue culture. | the dependence of epithelial spread on human serum in culture has been studied. using measured pieces of mouse ear skin epithelial outgrowth about floating explants (epiboly) and from adherent explants was studied. where compared directly the two systems show similar results. because of its ease of quantitation, the adherent explant culture was studied in greater detail. in this system in the presence of serum appreciable spread was found only after 48 h but spread continued for at least the nex ... | 1979 | 87150 |
| dicarbocyanine fluorescent probes of membrane potential block lymphocyte capping, deplete cellular atp and inhibit respiration of isolated mitochondria. | 3,3'-dipropylthiodicarbocyanine iodide, a widely used fluorescent probe of membrane potential, was found to inhibit anti-ig antibody, induced capping of mouse lymphocytes. the dye also lowered the cell atp content. experiments with isolated mitochondria revealed that the probe had a potent inhibitory action at site i of the respiratory chain. this mitochondrial blockade helps to explain the atp depletion and blockade of capping, and gives cause for caution in the use of this dye as a probe of ce ... | 1979 | 87222 |
| t cell-produced immunoglobulin binding factor (ibf) bears determinants coded for by the i region of the major histocompatibility complex and lacks allogeneic restriction. | an immunoglobulin binding factor (ibf) produced by in vivo alloantigen-activated t cells suppresses in vitro antibody production to sheep red blood cells (srbc) and probably represents the soluble form of the t cell fc receptor. to study the relationship between ibf and the major histocompatibility complex (mhc), two types of experiments were undertaken. first, different ibf were prepared by injecting thymocytes from mice of h-2k, h-2b and h-2d haplotypes into irradiated, allogeneic recipient mi ... | 1979 | 87328 |
| induction and characterization of isogeneic anti-idiotypic antibodies to balb/c myeloma s117: lack of reactivity with major idiotypic determinants. | isogeneic anti-idiotypic antibodies were induced by immunization of balb/c mice with the balb/c-derived myeloma protein s117 which binds n-acetylglucosamine-containing antigens including group a streptococcal carbohydrate (a-cho). most balb/c mice produced anti-s 117 idiotypic antibodies, as shown by various different immunization protocols. the antibodies of individual mice were of intermediate to high affinity (2.8 x 10(6) m-1 to 1.4 x 10(8) m-1). in isoelectric focusing, most individual antib ... | 1979 | 87329 |
| development and differentiation of mouse blastocysts in serum-free medium. | | 1979 | 87331 |
| a simple one-step procedure for staining the nucleolus organizer regions. | a simple silver staining technique for routine use is described by which the nucleolus organizer regions of mammalian chromosomes, including those of mouse chromosomes, are stained selectively. | 1979 | 87343 |
| phenotypic conversion of cultured mouse embryo cells by aza pyrimidine nucleosides. | | 1978 | 87352 |
| the effect of hapten-specific suppression of ige on antigen-induced histamine release from mouse peritoneal mast cells. | subcutaneous injections of a mixture of dinitrophenylated ovalbumin (dnp3-oa) and dextran sulfate into swiss-webster mice elicited short-lived primary and long-lasting secondary ige antibody responses to both dnp and oa. histamine was released on in vitro challenge with antigen (oa or dnp22-bsa) of washed peritoneal mast cells (pmc) obtained from mice during a primary or a secondary ige response. administration of an intravenous injection of a tolerogenic conjugate of dnp8-mouse gamma-globulin, ... | 1979 | 87380 |
| isolation of alpha-fetoprotein messenger rna from mouse yolk sac. | | 1979 | 87394 |
| plasmodium-infected blood cells analyzed and sorted by flow fluorimetry with the deoxyribonucleic acid binding dye 33258 hoechst. | red cells from plasmodium berghei infected mouse blood can be sorted on the basis of their dna content with the bisbenzimidazole dye 33258 hoechst. the optimal conditions for dye uptake have been established and with these conditions uninfected cells are nonfluorescent and can be completely separated from infected cells which exhibit fluorescence in almost direct proportion to the number of parasite nuclei (i.e. dna) they contain. the number of fluorescent cells detected and their fluorescence i ... | 1979 | 87413 |
| the fine specificity of regulatory t cells. i. hen egg-white lysozyme-induced suppressor t cells in a genetically nonresponder mouse strain do not recognize a closely related immunogenic lysozyme. | | 1979 | 87419 |
| mechanisms of mouse mast cell activation and inactivation for ige-mediated histamine release. | the ige-mediated histamine release from mouse mast cells requires ca++, is optimal at 37 degrees c, and is enhanced by phosphatidylserine. the rate of release is relatively slow. the mast cells can be activated to release histamine by either anti-ige or anti-fab antibodies and, in the case of cells from sensitized mice, by the immunizing antigen. the incubation of mast cells with antigen in the absence of ca++ or phosphatidylserine fails to release histamine. such cells are desensitized to the f ... | 1979 | 87441 |
| the activation of the alternative complement pathway by fetal calf serum and mouse thymocytes. | mouse thymocytes activated the alternative complement pathway of mouse serum in the presence of heated fetal calf serum. the activation required c3 from the fetal calf serum but was independent of antibody either in the murine or bovine serum. no other murine cells tested, including erythrocytes, peripheral blood lymphocytes, lymph node cells, spleen cells, and various cultured cell lines, activated the alternative complement pathway as effectively as thymocytes. in addition, sera from species o ... | 1979 | 87451 |
| properties and applications of monoclonal antibodies directed against determinants of they thy-1 locus. | fusion of cells of the mouse myeloma line, p3/x63-ag8 with spleen cells from akr/j mice immunized against c3h thymocytes or from (balb/c x balb.k)f1 mice immunized against akr/j thymocytes gave rise to hybrid cell lines that continuously secrete antibodies specific for the thy-1.2 and thy-1.1 antigens, respectively. monoclonal antibodies from four such cell lines were analyzed in detail. all were 19s igm, and, in the presence of complement (c), had high lytic titers on t cells of the appropriate ... | 1979 | 87465 |
| allotypes of c3 in laboratory and wild mouse distinguished by alloantisera. | | 1979 | 87473 |
| mechanisms of regulation of cell-mediated immunity. iv. azobenzenearsonate-specific suppressor factor(s) bear cross-reactive idiotypic determinants the expression of which is linked to the heavy-chain allotype linkage group of genes. | t-cell derived suppressor factor(s) (sf) specific for azobenzenearsonate (aba) were prepared by the mechanical disruption of suppressor cells. such suppressor factors were adsorbed to and recovered from immunoadsorbents prepared from the f(ab')2 fragments of rabbit immunoglobulin directed against the cross-reactive idiotype of a/j anti-aba antibodies. these aba-suppressor factors were not retained on sepharose 4b immunoadsorbent columns which had been coupled with f(ab')2 fragments or normal rab ... | 1979 | 87484 |
| passive transfer of lymphocytes from diabetic man to athymic mouse. | | 1979 | 87744 |
| passive transfer of lymphocytes from diabetic man to athymic mouse. | | 1979 | 87745 |
| passive transfer of lymphocytes from diabetic man to athymic mouse. | | 1979 | 87746 |
| cytotoxic t cells: lyt phenotype and blocking of killing activity by lyt antisera. | we reexamined two questions concerning lyt antigens of cytotoxic t cells of the mouse: is lyt-1 antigen expressed on cytotoxic effector cells and can cytotoxicity be blocked by antibody to lyt antigens in the absence of added complement? a 3-hr (51)cr-release assay with splenic effector cells and leukemia or myeloma target cells was used to measure cell-mediated cytotoxicity. the cytotoxic activity of effector cells against allogeneic targets was abolished by exposure to lyt-1, lyt-2, or lyt-3 a ... | 1979 | 88050 |
| surface mapping of mouse thymocytes. | the blocking method used previously for determining the relative positions of different components of the cell surface was modified by first fixing the cells with paraformaldehyde. this technique was applied to the h-2k (k), h-2d (d), tl, lyt-1, and lyt-2 surface components of mouse thymocytes, and the results were compared in parallel with data obtained with the original technique with unfixed cells. previous mapping data with unfixed cells, indicating the positions of these molecules relative ... | 1979 | 88052 |
| fine specificity of antibodies to poly(glu60ala30tyr10) produced by hybrid cell lines. | the polyethylene glycol-mediated cell fusion technique has been used to analyze the diversity of the antibody response to the terpolymer poly(glu60ala30tyr10)(gat). nine stable clones (all producing igm k anti-gat antibodies) were isolated from a fusion between p3-x63-ag8 myeloma cells and spleen cells from a dba/2 mouse sensitized to gat 5 days earlier. seven other clones (producing igg k anti-gat antibodies) were derived from another fusion between nsi myeloma cells and spleen cells of (c57bl/ ... | 1979 | 88055 |
| human breast carcinoma antigen is immunologically related to the polypeptide of the group-specific glycoprotein of mouse mammary tumor virus. | we have shown [mesa-tejada, r., keydar, i., ramanarayanan, m., ohno, t., fenoglio, c. & spiegelman, s. (1978) proc. natl. acad. sci. usa 75, 1529--1533] that an antigen immunologically related to gp52, a 52,000-dalton glycoprotein of the mouse mammary tumor virus, can be identified in sections of human breast cancer by means of an indirect immunoperoxidase technique. the specificity of the reaction was established by absorption experiments which revealed that only purified gp52, or material cont ... | 1979 | 88056 |
| different teratogenic efficacy to mouse fetal cns of 5-azacytidine in combination with x-irradiation depends on the sequence of successive application. | the single treatment of pregnant mice on day 12 post conception with 5-azacytidine (azacr), followed by a single irradiation dose of 200 rad two hours later, is exclusively neurotoxic to the fetus, as shown by a severe hypoplasia of the parieto-occipital regions of the telencephalon. this effect is explicable by the specific function of the mitotic cell population for the integrity of the cortex wall. combining these two hazards in the reverse manner, i.e., irradiation followed by azacr, resulte ... | 1979 | 88083 |
| [immunochemical study of the leukocyte myeloperoxidases from various sources]. | the antigenic difference between myeloperoxidases of human, rabbit, guinea pig, horse, dog, sheep and mouse leucocytes and horse radish peroxidase was investigated. by counterimmunoelectrophoresis with antiserum specific for human and mouse myeloperoxidase and horse radish peroxidase, the enzyme catalysing peroxidase reaction in leucocytes from the above sources was shown to possess species specificity and different antigenic composition. | 1979 | 88108 |
| non-histone chromatin proteins of mouse spleen cells during the primary immune response to sheep red blood cells and aggregated human gamma-globulin. | 1. the protein/dna ratio in chromatin of spleen cells increased during immunization; the ratio was the highest at the time of the maximum antibody synthesis, then decreased to the control values. 2. in the spleen cells of mice immunized with sheep red blood cells or aggregated human gamma-globulin, several characteristic fractions of non-histone chromatin proteins were preferentially synthesized: two antigen-specific fractions were observed in the phase of igm and igg synthesis, respectively, an ... | 1978 | 88139 |
| automotive exhaust and mouse activity: relationships between pollutant concentrations and decreases in wheel running. | groups of male and female mice inhaled either clean air, 100 ppm carbon monoxide, or light-irradiated and nonirradiated automotive exhaust containing nominally 25, 50, 75, or 100 ppm carbon monoxide in three tests with exposure lasting from 4 to 7 days. exhaust from a factory or lean-tuned engine in the first and third tests reversibly suppressed activity wheel running during exposure in mice of both sexes by as much as 78.3 and 83.1%, respectively. light-irradiated exhaust suppressed running mo ... | 1979 | 88208 |
| properties of a basement membrane-related glycoprotein synthesized in culture by a mouse embryonal carcinoma-derived cell line. | two glycoproteins, gp-1 and gp-2, have been isolated from an extracellular membrane synthesized in cell culture by an embryonal carcinoma-derived cell line. the amino acid and carbohydrate compositions have been determined. both proteins are rich in half-cystine residues and contain approximately 12-15% carbohydrate. antibodies have been obtained against one of the glycoproteins, gp-2, in rabbits. the antibody reacts with basement membranes from adult mouse and human kidney glomeruli and tubules ... | 1979 | 88263 |
| multiple genes coding for the androgen-regulated major urinary proteins of the mouse. | we have purified a cdna fragment complementary to the mrna coding for one of the major urinary proteins (mups) synthesized in the mouse liver. using this cdna as a hybridization probe, we have shown that the level of mup mrna is lower in the livers of females and castrated males than in those of males. the addition of testosterone to females and castrated males results in an increase in the concentration of the mrna to levels found in males. there are approximately 15 gene per haploid genome cod ... | 1979 | 88267 |
| binding of native and aggregated human gamma-globulin by mouse lymphoid cells and fibroblasts. | | 1979 | 88288 |
| the h-y antigen:production of antibodies, detection, and cross-reaction between mouse, rat, and human. | | 1978 | 88303 |
| termination of natural tolerance to alpha-foetoprotein in rats: study of cell-mediated immunity in the macrophage migration inhibition test. | immunization of rats with mouse alpha-foetoprotein has been earlier shown to induce the antibody response to self afp or rats. in this work a single injection of afpm resulted in termination of natural tolerance of effector t-cells to afpr, as shown by the macrophage migration inhibition test using peritoneal exudate cells from immunized animals. a significant reaction was elicited by both afpm and afpr in the course of primary, secondary and third immunization with afpm. pronounced mmi reaction ... | 1979 | 88377 |
| the presence of intervening sequences in the alpha-fetoprotein gene of the mouse. | | 1979 | 88451 |
| life history of mouse sperm protein. intratesticular stages. | a basic protein fraction, migrating as a single band in acetic acid-urea gel, distinct from histones, was isolated from mouse sperm collected from vasa deferentia and caudae epididymides and was used to immunize female rabbits. the presence of antibodies to the mouse sperm protein (msp) in the rabbit antisera was demonstrated by a cytoimmunofluorescence procedure using the cells of origin of the antigenic protein, the mature mouse sperm. the specificity of the antisera was verified by fluid and ... | 1979 | 88454 |
| tumor-specific immunity induced by somatic hybrids. i. lack of relationship between immunogenicity and tumorigenicity of selected hybrids. | hybrid clones were derived from fusion of tepc-15 plasmacytoma cells of balb/c mice with mouse l cells of c3h origin. the morphology, tumorigenicity, and immunogenicity of three representative clones were extensively studied. one clone (ltc-1) showed a morphology intermediate to that of either parental cell and possessed the highest tumorigenic and immunogenic properties. the other two clones displayed a "flat" morphology which differed significantly from that of either parent. one of these two, ... | 1979 | 88482 |