| investigation of citrullinemia type i variants by in vitro expression studies. | mild citrullinemia is an allelic variant of classical citrullinemia type i also caused by deficiency of the urea cycle enzyme argininosuccinate synthetase (ass). affected patients comprise a biochemical but no clinical phenotype. however, there is no reliable parameter allowing conclusions regarding the course of the disorder or its type of manifestation. the aim of this study was to test the importance of varying levels of ass residual activities for the severity at diagnosis. bacterial in vitr ... | 2008 | 18473344 |
| arginine deprivation as a targeted therapy for cancer. | certain cancers may be auxotrophic for a particular amino acid, and amino acid deprivation is one method to treat these tumors. arginine deprivation is a novel approach to target tumors which lack argininosuccinate synthetase (ass) expression. ass is a key enzyme which converts citrulline to arginine. tumors which usually do not express ass include melanoma, hepatocellular carcinoma, some mesotheliomas and some renal cell cancers. arginine can be degraded by several enzymes including arginine de ... | 2008 | 18473854 |
| [construction of an infectious clone of equine infectious anemia virus by n-glycosylation reverse-mutations]. | to elucidate the role of n-glycosylation in fetal donkey dermal cell (fdd)-attenuated equine infectious anemia virus (eiav), we constructed an n-glycosylation reverse-mutation molecular clone, plgn191n236n246. this viral molecular clone was derived from the infectious clone plgfd3-8 by site-directed mutagenesis. this clone was used to transfect fetal donkey dermal (fdd) cells. infectious characteristics of transfectants were monitored by rt-pcr, indirect immune fluorescence and reverse transcrip ... | 2008 | 18479052 |
| mechanical and genetic separation of aster- and midzone-positioned cytokinesis. | the mitotic spindle positions the cytokinesis furrow. the cytokinesis furrow then forms and ingresses at the site of the mitotic spindle, between the spindle poles. two populations of spindle microtubules are implicated in cytokinesis furrow positioning: radial microtubule arrays called asters and bundled non-kinetochore microtubules called the spindle midzone. here i will discuss our recent results that provided examples of how aster-positioned and midzone-positioned cytokinesis can be mechanic ... | 2008 | 18481963 |
| differences in ability of jennies and mares to conceive with cooled and frozen semen containing glycerol or not. | a suitable method for the cryopreservation of donkey semen would be very valuable for the ex situ management of genetic diversity in this species. this report uses a variety of observation and trials to evaluate the effect of cryoprotectants in per-cycle pregnancy rates (pc) in equids females (jennies (donkey) and mares (horse)). this was explored by (1) comparing the results of insemination of jennies and mares with cooled or frozen donkey semen, (2) examining the possible toxic effect of the c ... | 2009 | 18502059 |
| neurovirulence evaluation of venezuelan equine encephalitis (vee) vaccine candidate v3526 in nonhuman primates. | assessment of neurovirulence is a standard test for vaccines derived from virulent neurotropic viruses. this study evaluated the potential neurovirulence of v3526, a live attenuated vaccine derived from a full-length infectious clone of venezuelan equine encephalitis virus (veev) trinidad donkey strain (trd), a comparator veev vaccine (tc-83), trd, and process control material (pcm) in juvenile rhesus macaques. following intrathalamic/intraspinal (i.t./i.s. ) or subcutaneous (s.c.) inoculations, ... | 2008 | 18508163 |
| ass melt under pressure: one substance, three liquids. | an in situ high-temperature--high-pressure study of liquid chalcogenide ass by x-ray diffraction, resistivity measurements, and quenching from melt is presented. the obtained data provide direct evidence for the existence in the melt under compression of two transformations: one is from a moderate-viscosity molecular liquid to a high-viscosity nonmetallic polymerized liquid at p approximately 1.6-2.2 gpa; the other is from the latter to a low-viscosity metallic liquid at p approximately 4.6-4.8 ... | 2008 | 18518048 |
| structure of the o-polysaccharide of the lipopolysaccharide produced by taylorella asinigenitalis type strain (atcc 700933). | taylorella asinigenitalis sp. nov is a nonpathogenic gram-negative bacterium recently isolated from the genital tract of male donkeys. the bacterium is phenotypically indistinguishable from taylorella equigenitalis, a pathogen that is the cause of contagious equine metritis, a highly communicable venereal disease of horses. the structural analysis of the lipopolysaccharide produced by t. asinigenitalis sp. nov (atcc 700933) demonstrated that its o-polysaccharide (o-ps) component is a linear unbr ... | 2008 | 18523489 |
| [character study of content and correlativity of elements in radix astragali obtained from different regions]. | to study the characters of concentration of 42 elements in radix astragali and make an attempt at looking for relationship between the element concentration and regions where samplers are obtained. | 2008 | 18536459 |
| elastic and macroporous agarose-gelatin cryogels with isotropic and anisotropic porosity for tissue engineering. | the focus of this work was to design a macroporous scaffold with controlled porosity in isotropic and anisotropic manner for tissue-engineering applications. agarose-gelatin scaffolds were synthesized by cryogelation method, in which agarose was used to improve the mechanical characteristics and gelatin-provided amiable property of elasticity, cell adhesion, and cell proliferation in the scaffold. agarose-gelatin (8%) cryogels synthesized in two different solvent systems (i.e., water and 0.1% ac ... | 2009 | 18563830 |
| histomorphometric analysis of the irides of dogs, camels, buffalos and donkeys. | the present study was carried out to investigate the morphological and histomorphometrical characters of irides in dogs, camels, buffalos, and donkeys. the findings of the study revealed that, morphologically, the irides were consisted of an anterior border layer, a middle layer of connective tissue stroma and a posterior layer of pigmented epithelium. interestingly, the anterior borders of all investigated animals were not enveloped by a distinct epithelial or endothelial lining, but on contrar ... | 2009 | 18565555 |
| isolation of enterobacter sakazakii from ass' milk in sicily: case report, safety and legal issues. | enterobacter sakazakii (es) infections are likely to involve newborns and infants, causing meningitis and necrotizing enterocolitis and sepsis. contamination of infant formulae milk during factory production or bottle preparation is implicated. es has been isolated from environmental sources and from food other than infant formula and milk powder, but why it is associated only with the consumption of infant formulae, is unclear. according to regulation (ec) no. 2073/2005 on the microbiological c ... | 2008 | 18571118 |
| [ass plus dipyridamole or clopidogrel after stroke: what is the best secondary prevention?]. | | 2008 | 18575244 |
| effects of freezing/thawing on motile sperm subpopulations of boar and donkey ejaculates. | the main aim of this study is to assess the influence of freeze/thawing on motile sperm subpopulations in ejaculates from two phylogenetically different mammalian species, boar and donkey. our results indicate that, whereas boar and donkey sperm respond very differently in their mean motion characteristics to freezing/thawing, this process did not change the existence of a 4-subpopulations structure in the ejaculates in either species when these subpopulations were defined by taking values of cu ... | 2008 | 18602684 |
| potential of supramolecular solvents for the extraction of contaminants in liquid foods. | amphiphile-based supramolecular solvents (asss), which are water immiscible liquids consisting of supramolecular aggregates in the nano- and micro-scale regimes dispersed in a continuous phase, were assessed for the extraction of trace contaminants in liquid foods. the ass selected was made up of reversed micelles of decanoic dispersed in tetrahydrofuran (thf)-water and the contaminants used as a model were bisphenol a (bpa), ochratoxin a (ota) and benzo(a)pyrene (bapy). the influence of matrix ... | 2009 | 18603255 |
| illustrated identification keys to strongylid parasites (strongylidae: nematoda) of horses, zebras and asses (equidae). | the equidae (the horse, equus caballus, the ass, equus asinus, zebras and their hybrids) are hosts to a great variety of nematode parasites, some of which can cause significant morbidity or mortality if individual hosts are untreated. worldwide the nematode parasites of horses belong to 7 suborders, 12 families, 29 genera and 83 species. the great majority (19 of 29 genera and 64 of 83 species) are members of the family strongylidae, which includes the most common and pathogenic nematode parasit ... | 2008 | 18603375 |
| a disruption of ctpa encoding carboxy-terminal protease attenuates burkholderia mallei and induces partial protection in cd1 mice. | burkholderia mallei is the etiologic agent of glanders in solipeds (horses, mules and donkeys), and incidentally in carnivores and humans. little is known about the molecular mechanisms of b. mallei pathogenesis. the putative carboxy-terminal processing protease (ctpa) of b. mallei is a member of a novel family of endoproteases involved in the maturation of proteins destined for the cell envelope. all species and isolates of burkholderia carry a highly conserved copy of ctpa. we studied the invo ... | 2008 | 18614331 |
| tetanus in the equine species: a retrospective study of 31 cases. | few studies exist about factors affecting the outcome of horses with tetanus. | 2008 | 18615902 |
| haplotypic variability within the mitochondrial gene encoding for the cytochrome c oxidase 1 (cox1) of cylicocyclus nassatus (nematoda, strongylida): evidence for an affiliation between parasitic populations and domestic and wild equid hosts. | this study investigated the genetic variability within cylicocyclus nassatus (nematoda, strongylida, cyathostominae) collected from different domestic and wild hosts (i.e. horse, donkey, przewalskii horse, tarpan and turkmen kulan) and localities in europe and/or usa. the ribosomal internal transcribed spacer 2 (its2) and the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene were pcr-amplified and sequences characterized from seventy individual parasitic specimens. while its2 displayed 0- ... | 2008 | 18619736 |
| the rabies situation in the middle east. | rabies is a public health problem of significant importance in the majority of southern and eastern mediterranean and middle eastern countries. in some of these countries, there is a considerable death rate due to rabies. dogs are the main source of human infection, while cats constitute the second most important group of domestic animals followed by cattle, sheep, goats, camels, donkeys and then wild animals. there are around 300 reported human cases in these regions annually, with several hund ... | 2008 | 18634465 |
| mule cognition: a case of hybrid vigour? | this study compares the behaviour of the mule (equus asinus x equus caballus) with that of its parent species to assess the effects of hybridization on cognition. six mules, six ponies (e. caballus) and six donkeys (e. asinus) were given a two choice visual discrimination learning task. each session consisted of 12 trials and pass level was reached when subjects chose the correct stimulus for at least 9 out of the 12 trials in three consecutive sessions. a record was made of how many pairs each ... | 2009 | 18636282 |
| prevalence of equine herpesvirus-1 and equine herpesvirus-4 infections in equidae species in turkey as determined by elisa and multiplex nested pcr. | in this report we examined the presence of specific antibodies against equine herpesvirus type 1 (ehv-1), and equine herpesvirus type 4 (ehv-4) in several equidae, including mules, donkeys, horses. the presence of ehv-1 and ehv-4 in respiratory diseases of equids, and ability of multiplex nested polymerase chain reaction (pcr) screening in simultaneous diagnosis of horses acutely infected by ehv-1 and ehv-4 were also investigated. sera from 504 horses, mules and donkeys sampled were tested for t ... | 2009 | 18649902 |
| aeuass3 (a = li, k, rb, and cs): new as3+ species from an arsenic-rich polysulfide flux. | four europium compounds, lieuass 3 ( i), keuass 3 ( iia), rbeuass 3 ( iib), and cseuass 3 ( iic), containing as (3+) were synthesized in molten alkali-metal polysulfide salts. an as-rich flux was found to be necessary to stabilize the pyramidal building unit [ass 3] (3-). all crystallize in the monoclinic space group p2 1/ c with two new structure types. compound i features a new coordination mode for the [ass 3] (3-) unit and three-dimensional [eu(ass 3)] (-) framework. compounds iia- c are iso ... | 2008 | 18652447 |
| pancreatic cancer cell lines deficient in argininosuccinate synthetase are sensitive to arginine deprivation by arginine deiminase. | eukaryotic cells can synthesize the non-essential amino acid arginine from aspartate and citrulline using the enzyme argininosuccinate synthetase (ass). it has been observed that ass is underexpressed in various types of cancers ass, for which arginine become auxotrophic. arginine deiminase (adi) is a prokaryotic enzyme that metabolizes arginine to citrulline and has been found to inhibit melanoma and hepatoma cancer cells deficient of ass. we tested the hypothesis that pancreatic cancers have l ... | 2008 | 18661517 |
| unusual transmission route of lymphogranuloma venereum; following sexual contact with a female donkey. | here, we present a 20-year-old man who presented with painful inguinal and femoral masses. he gave a history of sexual contact with a mare 14 days before his recent illness. he was diagnosed with lymphogranuloma venereum based on the histopathological findings and a high titre of igg (1:1400). | 2008 | 18663048 |
| glycosylation at the fetomaternal interface: does the glycocode play a critical role in implantation? | during pregnancy, the heavily glycosylated surfaces of the implanting blastocyst and maternal uterine epithelium interact in a highly controlled and specific manner. examination of this interface in species that show interdigitation of embryonic and maternal surfaces (epitheliochorial placentation) shows that each has its own particular pattern of glycosylation or glycotype, and that closely related and/or interbreeding species e.g. horse and donkey or llama and guanaco, have very similar glycot ... | 2009 | 18677581 |
| oestrus cycle characteristics and prediction of ovulation in catalonian jennies. | the catalonian donkey breed is in danger of extinction, and much needs to be learned about the reproductive features of its females if breeding and conservation programmes are to be successful. this study reports the oestrous behaviour, oestrus cycle characteristics and dynamic ovarian events witnessed during 50 oestrous cycles (involving 106 ovulations) in 10 catalonian jennies between march 2002 and january 2005. these jennies were teased, palpated transrectally and examined by ultrasound usin ... | 2008 | 18678401 |
| [a flow cytometric assay for the expression of interferon gamma in t lymphocytes and its application in the study of eiav-induced immune response]. | the attenuated vaccine of equine infectious anemia virus (eiav) is the first lentiviral vaccine that provides solid protection against the infection of eiav virulent strains. study of the immune response induced by eiav vaccine is an important approach to understand the immunity to other lentiviruses. ifn-gamma expressed by specifically stimulated lymphocytes is an important indicator for the evaluation of t cell-mediated immunity. a flow cytometry based assay was established in this study to ac ... | 2008 | 18720846 |
| polymorphisms in the kappa casein (csn3) gene in horse and comparative analysis of its promoter and coding region. | the major parts of the coding region and promoter of the equine kappa casein (csn3) gene were sequenced and compared among several species. four snps were identified in the csn3 gene: two in exon 1 and two in exon 4. the snps were genotyped in six slovenian horse breeds using rflp and two different pcr-based methods. the highest variation in genotype frequencies was found in the slovenian cold-blood breed. the snps in exon 4 may cause a change in the amino acid sequence and may alter chemical/fu ... | 2008 | 18752583 |
| human whole blood assay for rapid and routine testing of non-steroidal anti-inflammatory drugs (nsaids) on cyclo-oxygenase-2 activity. | aim of this study was to present a simple, fast and reliable method to examine the capacity of nsaids to inhibiting cox-2 activity that uses rapid (stimulation takes only 5 h compared to other existing protocols) and routine testing. the assay includes elimination of cox-1-activity using ass (a selective cox-1 inhibitor) and the thromboxane synthetase inhibitor (txbsi), cox-2 induction via lps and measurement of pge(2). using txbsi reduces the amount of lps and results in higher prostaglandin pr ... | 2008 | 18759073 |
| proviral genomic sequence analysis of chinese donkey leukocyte attenuated equine infectious anemia virus vaccine and its parental virus strain liaoning. | proviral dna was extracted from donkey leukocyte infected with chinese donkey leukocyte attenuated equine infectious anemia virus (dla-eiav), and peripheral blood lymphocytes (pbl) from a horse infected with the virulent eiav strain liaoning (eiav l). the entire proviral dna from both viruses was cloned and sequenced. the lengths of complete genomic sequences of dla-eiav and eiav l provirus were 8266 bp and 8235 bp, respectively. sequence comparison indicated that dla-eiav shares 97.0% and 97.5% ... | 2002 | 18763064 |
| maintenance of platelet in vitro properties during 7-day storage in m-sol with a 30-hour interruption of agitation. | extensive periods without agitation can occasionally occur during platelet (plt) shipment and can affect plt quality during 5- to 7-day storage. the use of buffer-containing plt additive solutions (ass) may better preserve plt quality during storage by maintaining plt ph and other in vitro variables. a newly described bicarbonate-containing as, m-sol, was compared to plasma for preservation of whole blood-derived plt concentrates in which a 30-hour interruption of agitation was included. | 2008 | 18764828 |
| intracellular aß triggers neuron loss in the cholinergic system of the app/ps1ki mouse model of alzheimer's disease. | loss of cholinergic neurons in the nucleus basalis of meynert in alzheimer's disease (ad) patients was one of the first discoveries of neuron loss in ad. despite an intense focus on the cholinergic system in ad, the reason for this cholinergic neuron loss is yet unknown. in the present study we examined abeta-induced pathology and neuron loss in the cholinergic system of the bigenic app/ps1ki mouse model. expression of the app transgene was found in chat-positive neurons of motor nuclei accompan ... | 2010 | 18771817 |
| pathologic findings in equine muscle (excluding polysaccharide storage): a necropsy study. | gross and histopathologic evaluation of skeletal muscle was performed in 229 equids (217 horses, 8 ponies, 3 donkeys, and 1 mule) 1 year of age or older undergoing postmortem examination at oregon state university in a 2.5-year period. animals were evaluated for grossly evident muscle lesions, and muscle samples were fixed in formalin, processed routinely, and stained with hematoxylin and eosin (he) and periodic acid-schiff (pas) for glycogen. muscle lesions were detected in 149 animals (65%). c ... | 2008 | 18776088 |
| matrix metallopeptidase 2 activity in tendon regions: effects of mechanical loading exercise associated to anabolic-androgenic steroids. | matrix metallopeptidases (mmps) are responsible for degradation of the extracellular matrix components and tissue remodeling. to achieve a better understanding of aas effects in rat tendon, mmp-2 activity in the proximal and distal regions of the calcanear tendon (ct) and proximal, intermediate and distal region of superficial (sft) and deep flexor tendons (dft) after mechanical load exercise associated with aas was investigated. animals were grouped into four groups: sedentary animals (s); sede ... | 2008 | 18810485 |
| immunohistochemical localisation of intravitreally injected bevacizumab at the posterior pole of the primate eye: implication for the treatment of retinal vein occlusion. | to locate bevacizumab in the posterior pole within 1-14 days after intravitreal injection in the primate eye. | 2008 | 18815425 |
| the relationship between kcl infusion and changes of ecg, electrolytes of plasma and k content of donkey's red blood cells. | sudden marked increases in the serum potassium concentration, up to 8 to 9 meq l(-1), are the result of transcellular movement of potassium and are associated with profound electrocardiographic abnormalities and fluid shifts. electrocardiographic changes associated with hyperkalemia have been described in the cat, the dog, the horse and are well documented in man. since there is no research about the effects of hyperkalemia on electrocardiogram of donkey, the purpose of this study was to induce ... | 2008 | 18817168 |
| analysis of gene expression identifies differentially expressed genes and pathways associated with lymphatic dissemination in patients with adenocarcinoma of the esophagus. | the presence of lymphatic dissemination is an important predictor of survival in esophageal adenocarcinoma (ea). the aim of this study was to discover a prognostic gene expression profile for lymphatic dissemination in ea and to identify genes and pathways that provide oncological insight in lymphatic dissemination. | 2008 | 18825457 |
| selective stimulation of either tumor necrosis factor receptor differentially induces pain behavior in vivo and ectopic activity in sensory neurons in vitro. | recent studies suggest that tumor necrosis factor-alpha (tnf) sensitizes primary afferent neurons, and thus facilitates neuropathic pain. here, we separately examined the roles of tumor necrosis factor receptor (tnfr) 1 and 2 by parallel in vivo and in vitro paradigms using proteins that selectively activate tnfr1 or tnfr2 (r1 and r2). in vivo, intrathecally injected r1, but not r2 slightly reduced mechanical and thermal withdrawal thresholds in rats, whereas co-injection resulted in robust, at ... | 2008 | 18838115 |
| identification of a liver-specific camp response element in the human argininosuccinate synthetase gene. | argininosuccinate synthetase (ass), a key enzyme in the urea cycle, participates in many metabolic processes including arginine biosynthesis and the citrulline-nitric oxide (no) cycle. factors like diets, hormones and pro-inflammatory stimuli are known to regulate ass gene expression primarily at the transcription level. however, little is known about the cis-elements for transcriptional regulation of the ass gene. in this study, we employed dnase i hypersensitive sites mapping to identify poten ... | 2008 | 18840401 |
| cases of fistulous withers in a horse and a donkey in egypt. | | 1948 | 18863545 |
| the role of molecular testing and enzyme analysis in the management of hypomorphic citrullinemia. | expanded newborn screening detects patients with modest elevations in citrulline; however it is currently unclear how to treat these patients and how to counsel their parents. in order to begin to address these issues, we compared the clinical, biochemical, and molecular features of 10 patients with mildly elevated citrulline levels. three patients presented with clinical illness whereas seven came to attention as a result of expanded newborn screening. one patient presented during pregnancy and ... | 2008 | 18925679 |
| using indirect elisa to assess different antigens for the serodiagnosis of fasciola gigantica infection in cattle, sheep and donkeys. | an indirect enzyme-linked immunosorbent assay (ielisa) was evaluated for its diagnostic capability in detecting antibodies against fasciola gigantica infection in cattle, sheep and donkeys sera using crude worm, excretory-secretory and glutathione s-transferase antigens prepared from adult liver fluke. presence of f. gigantica worms at post-mortem examination of cattle, sheep and donkey's livers was taken as a gold standard for the evaluation of the assay. the diagnostic sensitivity, specificity ... | 2009 | 18947842 |
| cervical hypoplasia in jennets: a description of two cases. | the authors describe two cases of cervical hypoplasia in jennets, detected during the gynaecological evaluation of two purebred females of the portuguese donkey breed asinina de miranda. these jennets aged 10 and 15 years were referred for consultation by the local breeders association because of infertility detected during an artificial breeding programme. in both jennets the cervix was small, shorter than the usual and closed. in one of the females, outflow of semen during artificial inseminat ... | 2010 | 18954379 |
| the study of sodium channels involved in pain responses using specific modulators. | voltage-gated sodium channels (vgscs) are transmembrane proteins responsible for generation and conduction of action potentials in excitable cells. physiological and pharmacological studies have demonstrated that vgscs play a critical role in chronic pain associated with tissue or nerve injury. many long-chain peptide toxins (60-76 amino acid residues) purified from the venom of asian scorpion buthus martensii karsch (bmk) are investigated to be sodium channel-specific modulators. the alpha-like ... | 2008 | 18958371 |
| precise and accurate determination of high concentrations of sulphur by isotope-dilution thermal-ionization mass-spectrometry. | an isotope-dilution thermal-ionization mass-spectrometric procedure has been developed for the accurate and precise determination of sulphur in steels and organic-based materials. the sample and isotopically enriched sulphur spike are dissolved in a sealed tube to prevent loss of sulphur and the sulphur isotopes are measured as ass(+) ions, with silica gel as an emitter. this technique has been applied to the determination of sulphur in 13 nbs standard reference materials ranging in concentratio ... | 1984 | 18963726 |
| solvent extraction of 8-quinolinolato-iron(iii) with the aid of various phenols as hydrogen-bonding donors. | effect of various phenols (aroh) on the solvent extraction of iron(iii) with 8-quinolinol (hq) has been investigated. greatly enhanced extraction is found in the presence of aroh, e.g., the distribution ratio of iron(iii) with hq in carbon tetrachloride is increased 200,000-fold by 0.10m 3,5-dichlorophenol. from extraction equilibrium analysis, the enhanced extraction has been ascribed to the formation of association complexes of neutral iron(iii) 8-quinolinolate (feq(3)) with aroh as feq(3) . n ... | 1993 | 18965621 |
| determination of gold in biological materials by electrothermal atomic absorption spectrometry with a molybdenum tube atomizer. | electrothermal atomic absorption spectrometry (eta-ass) of gold with a molybdenum tube atomizer has been investigated. a sensitive eta-aas method was developed. the gold absorption signal became higher with the heating rate of the tube atomizer and as the ratio of hydrogen in the argon purge gas decreased. the optimal heating rate and the optimal gas flow rate were 5.5 c/msec and ar 480 ml/min + h(2) 20 ml/min, respectively. the absolute characteristic mass (the mass of element giving 0.0044 abs ... | 1995 | 18966226 |
| tensammetric determination of non-ionic surfactants combined with bias separation procedure (wickbold) 2. optimisation of the precipitation and investigation of interferences. | the precipitation step in the developed procedure (bias-itm) was optimised with the aim of achieving a lower detection limit. losses during filtration using triton x-100 as representative non-ionic surfactant (ns), the indirect tensammetric method (itm) and adsorptive stripping tensammetry (adst) for the determination of the concentration of the surfactant in the precipitate and in the filtrate were investigated. the g4 glass filter recommended up to now was found to be insufficiently effective ... | 1995 | 18966351 |
| studies on the extraction of copper(ii) with 4-alkyldithiocarboxylate derivatives of 1-phenyl-3,5-dioxopyrazolidine. | the extraction behaviour of copper(ii) from acid solutions (ph 0-6) was studied with the new reagent 4-alkyldithiocarboxylate-3,5-dihydroxy-l-phenylpyrazol (hl; alkyl = n-butyl, n-dodecyl). the species extracted was found to be ml(2). the reagent was used to separate copper(ii) from iron(iii) in an acidic lixiviation solution of a mineral sample composed of enarguite (cu(3)ass(4)) and pyrite (fes(2)). additionally, the extraction efficiency of this new reagent was compared with the commercially ... | 1996 | 18966517 |
| electrochemical and thermodynamic studies of the ion-pair formation of chloropentamminecobalt(iii) ion in ethyl alcohol-water media containing different dicarboxylate ligands. | the ion-pair dissociation constants, k(d), of the ion-pair formed between chloropentamminecobalt(iii) ion (cpx(2+)) and a variety of dicarboxylate ligands, have been determined from emf measurements of a cell composed of glass and calomel electrodes. measurements were made in water and in aqueous binary mixtures of ethyl alcohol, over a wide range of solvent composition (0-60 wt% ethyl alcohol), at six different temperatures (ranging from 30 to 55 degrees c at intervals of 5 degrees c). the ther ... | 1998 | 18967072 |
| association constants of dibenzo[3n + 2]crown-n ethers using steady-state fluorescence spectroscopy. | the steady-state fluorescence spectra of cation complexes of fluorophore macrocyclic ethers have been studied for the estimation of 1:1 association constants, and perchlorate salts of li(+), na(+), k(+) rb(+) and pb(2+) complexing with dibenzo[23]crown-9, dibenzo[26]crown-10, and sym-dibenzo[26]crown-10, were investigated. the fluorescence emission maximum of the free and the various ligand/cation mixtures of complexed crown ethers were measured at room temperature in an. the concentrations of c ... | 2005 | 18969874 |
| pathological investigation of caries and occlusal pulpar exposure in donkey cheek teeth using computerised axial tomography with histological and ultrastructural examinations. | post-mortem examination of 16 donkey cheek teeth (ct) with caries (both peripheral and infundibular) and pulpar exposure were performed using computerised axial tomography (cat), histology and scanning electron microscopy. cat imaging was found to be useful to assess the presence and extent of caries and pulp exposure in individual donkey ct. histology identified the loss of occlusal secondary dentine, and showed pulp necrosis in teeth with pulpar exposure. viable pulp was present more apically ... | 2008 | 18980849 |
| [chronic myeloproliferative diseases. current therapeutic standards and new developments]. | chronic myeloproliferative diseases (cmpd) are haematopoetic neoplasias with indolent course and preserved cellular function of the maturing malignant cells. in philadelphia-positive chronic myeloid leukaemia the discovery of molecular disease mechanisms led to the successful introduction of targeted therapy with imatinib. ph-negative cmpd are conventionally treated by cytoreduction and low dose ass in order to minimise the risk of vascular complications. for all cmpd, lifelong surveillance and ... | 2008 | 18985309 |
| when arsine makes the difference: chelating phosphino and bridging arsinoarylthiolato gallium complexes. | the (organo)gallium compounds gacl{(sc6h4-2-pph2)-kappa2s,p}2 (1), ga{(sc6h4-2-pph2)-kappa2s,p}{(sc6h4-2-pph2)-kappas}2 (2), game2{(sc6h4-2-pph2)-kappa2s,p} (3), gatbu2{(sc6h4-2-pph2)-kappa2s,p} (4), gatbu{(sc6h4-2-pph2)-kappa2s,p}{(sc6h4-2-pph2)-kappas} (5), [game2{(mu2-sc6h4-2-asph2)-kappas}]2 (6), and gatbu{(sc6h4-2-asph2)-kappa2s,as}{(sc6h4-2-asph2)-kappas} (7) were obtained from the reaction of 2-eph2c6h4sh (e = p (psh), as (assh)) with gacl3 (1, 2) or gar3 (r = me, tbu; 3-7) in different m ... | 2008 | 18986138 |
| herpesvirus-associated neurological disease in a donkey. | a 4-year-old donkey was evaluated for progressive neurological abnormalities consisting of depression, stupor, weakness, and recumbency. diagnostic evaluation for viral involvement identified an asinine herpesvirus in dna extracted from deep pharyngeal swabs. specific primers were designed based on comparison with equine herpesviral dna polymerase sequences and yielded an 875-base pair product from the donkey. this sequence had complete identity with short sequences of asinine herpesvirus previo ... | 2008 | 18987238 |
| source and release mechanism of arsenic in aquifers of the mekong delta, vietnam. | in order to elucidate the arsenic source and its release mechanism into groundwater in the mekong delta, vietnam, groundwater samples were collected from wells at different depths (20 to 440 m) and core samples (from 20 to 265 m depth) were analyzed. based on the analytical results for groundwater and core samples, the as source in groundwater is considered to be pyrite (fes(2)) in acid sulfate soil (ass) under oxidizing conditions and hydrous ferric oxide (fe(oh)(3)) under reducing conditions. ... | 2009 | 18996614 |
| the human neonatal small intestine has the potential for arginine synthesis; developmental changes in the expression of arginine-synthesizing and -catabolizing enzymes. | milk contains too little arginine for normal growth, but its precursors proline and glutamine are abundant; the small intestine of rodents and piglets produces arginine from proline during the suckling period; and parenterally fed premature human neonates frequently suffer from hypoargininemia. these findings raise the question whether the neonatal human small intestine also expresses the enzymes that enable the synthesis of arginine from proline and/or glutamine. carbamoylphosphate synthetase ( ... | 2008 | 19000307 |
| gross anatomy of the female genital organs of the domestic donkey (equus asinus linné, 1758). | although donkeys play an important role as companion or pack and draught animals, theriogenological studies and anatomical data on the genital organs of the jenny are sparse. to provide anatomical descriptions and morphometric data, the organa genitalia feminina, their arteries and the ligamentum latum uteri of 10 adult but maiden jennies were examined by means of gross anatomical and morphometric techniques. in comparison with anatomical data of horses obtained from literature the genital organ ... | 2009 | 19007353 |
| ocular infection of donkeys (equus asinus) with setaria equina. | seven donkeys raised in different locations in egypt were found to have a unilateral eye showing motile white worms in the aqueous humor. the parasites were surgically removed from the anterior chamber of the eye in five out of the seven donkeys, and were cleared, mounted and identified as setaria equina based on light microscopic features. the ocular infection with s. equina reported herein may be the first reported aberrant cases in the egyptian animals. | 2009 | 19015947 |
| assessment of sperm dna fragmentation in stallion (equus caballus) and donkey (equus asinus) using the sperm chromatin dispersion test. | sperm dna fragmentation (sdf) is an important parameter to assessing sperm quality. information about sperm quality is not available for donkeys, especially in some breeds at risk of extinction. the objectives of this research were to test the four commercial variants of sperm chromatin dispersion test (scd; sperm halomax test), originally developed to assess sdf in boars, bulls, rams and stallions, in order to scrutinize their applicability in the study of sdf in a donkey breed at risk of extin ... | 2009 | 19019071 |
| assessment of urinary total testosterone production by a highly sensitive time-resolved fluorescence immunoassay. | radioimmunoassay (ria) that involves purification of the analyte by organic solvent extraction is widely used. although the extraction ria is reliable when properly validated, it is time consuming and radioactive, we measure urinary total testosterone with a highly sensitive rapid and accurate time-resolved fluorescence immunoassay (trfia) method. high affinity antitestosterone antibody and eu(3+) labeled donkey antisheep igg as tracers were used. the assay was evaluated for specificity, sensiti ... | 2008 | 19021270 |
| citrin deficiency, a perplexing global disorder. | citrin deficiency, caused by mutations in slc25a13, can present with neonatal intrahepatic cholestasis or with adult onset neuropsychiatric, hepatic and pancreatic disease. until recently, it had been thought to be found mostly in individuals of east asian ancestry. a key diagnostic feature has been the deficient argininosuccinate synthetase (ass) activity (e.c. 6.3.4.5) in liver, with normal activity in skin fibroblasts. in this series we describe the clinical presentation of 10 patients referr ... | 2009 | 19036621 |
| phylogenetic analysis of west nile virus isolated in italy in 2008. | in italy the first occurrence of west nile virus (wnv) infection was reported in tuscany region during the late summer of 1998. in august 2008, the wnv infection re-emerged in italy, in areas surrounding the po river delta, and involving three regions lombardy, emilia romagna and veneto. wnv was isolated from blood and organs samples of one horse, one donkey, one pigeon (columba livia) and three magpies (pica pica). the phylogenetic analysis of the isolates, conducted on 255 bp in the region cod ... | 2008 | 19040827 |
| morphology and diagnosis of some fourth-stage larvae of cyathostomines (nematoda: strongyloidea) in donkeys equus asinus l. from ethiopia. | fourth-stage larvae of four species of the cyathostominae nicoll, 1927 parasitic in donkeys equus asinus l. from ethiopia were identified mainly using moulting specimens. they are cylicocyclus asini matthee, krecek & gibbons, 2001, c. auriculatus (looss, 1900) chaves, 1930, cyathostomum tetracanthum (mehlis, 1831) molin, 1861 (sensu looss, 1900) and cylindropharynx brevicauda leiper, 1911. the larva of cylicocyclus asini is similar to those of c. nassatus (looss, 1900) chaves, 1930 and c. leptos ... | 2009 | 19048404 |
| application of 1-alkyl-3-methylimidazolium-based ionic liquids as background electrolyte in capillary zone electrophoresis for the simultaneous determination of five anthraquinones in rhubarb. | a capillary zone electrophoresis method using only 1-alkyl-3-methylimidazolium-based ionic liquids as background electrolyte for the simultaneous determination of five anthraquinone derivatives including aloe-emodin, emodin, chrysophanol, physcion and rhein in rhubarb species was described. ion association constants, k(ass), between anthraquinone anions and imidazolium cations were determined by analyzing the electrophoretic mobility change of anthraquinone anions using a non-linear least-square ... | 2007 | 19071659 |
| expression of interleukin-1 receptor antagonist in human cornea. | the purpose of this study was to confirm the expression of interleukin-1 receptor antagonist (il-1 ra) in the human cornea. four samples of human ex vivo corneal epithelium were obtained from patients undergoing photorefractive keratectomy. rt-pcr was performed using mrna isolated from the corneal epithelium and oligo-dt primers. pcr was performed on the cdna products using primers specific for human il-1 ra. the pcr products were subcloned and sequenced. human cornea sections were prepared from ... | 2009 | 19087877 |
| a phytosociological research on the vegetation of ahirdaği (afyonkarahisar). | this phytosociological study was carried out to determine the vegetation of ahirdaği. the study distinguished four plant communities distributed among three different types of vegetation. the vegetation tables are included. relevant types and associated syntaxa are as follows: forest and shrub vegetation [quercetea pubescentis (oberd, 1948) doing kraft, 1955; querco-cedretalia libani barbero, loisel et quézel, 1974; carpino-acerion quézel, barbero and akman, 1978; 1- ferulagini macrosciadiae-pin ... | 2007 | 19090142 |
| strong second harmonic generation from the tantalum thioarsenates a3ta2ass11 (a = k and rb). | the strongly anisotropic thioarsenates a(3)ta(2)ass(11) are stabilized in a polysulfide flux. all compounds contain the same parallel (1)/(infinity)[ta(2)ass(11)(3-)] polymeric anionic chains, but the size of the alkali-metals has a profound effect on the packing of the chains. the k(+) or rb(+) favor noncentrosymmetric packing of the chains, whereas the larger cs(+) favors the centrosymmetric packing. the chains feature the combination of two asymmetric units [ta(2)s(11)] and [ass(3)] and exhib ... | 2009 | 19090658 |
| prevalence of methicillin-resistant staphylococcus pseudintermedius isolated from clinical samples of companion animals and equidaes. | in this study the prevalence of methicillin-resistant staphylococcus pseudintermedius (mrsp) in clinical specimens of different animal species was defined by investigating a total of 16,103 clinical samples originating from veterinary facilities of five german federal states in 2007. of all samples examined, 72 were positive for mrsp, giving an overall prevalence of 0.45%. in clinical specimens originating from small animals the prevalence was 0.58% (n=67; dogs n=61 and cats n=6), while samples ... | 2009 | 19097710 |
| hydatidosis of slaughtered animals in ngorongoro district of arusha region, tanzania. | a study on the prevalence of hydatidosis in cattle, goats and sheep was carried out in ngorongoro district of arusha region, tanzania. a 4-years data records from four slaughter slabs were retrieved and analysed. in addition, meat inspection was done in the same slaughter slabs for nine months and 64 households were interviewed to assess the community awareness on hydatidosis. results showed the overall prevalence of hydatidosis to be 47.9%. species prevalence of 48.7%, 34.7% and 63.8% in cattle ... | 2009 | 19105043 |
| interhemispheric distribution of alzheimer disease and vascular pathology in brain aging. | most of the neuropathological studies in brain aging were based on the assumption of a symmetrical right-left hemisphere distribution of both alzheimer disease and vascular pathology. to explore the impact of asymmetrical lesion formation on cognition, we performed a clinicopathological analysis of 153 cases with mixed pathology except macroinfarcts. | 2009 | 19118241 |
| the basis for disease-modifying treatments for alzheimer's disease: the sixth annual mild cognitive impairment symposium. | at the sixth annual mild cognitive impairment symposium in miami beach, florida, a multidisciplinary group of experts from the alzheimer's disease (ad) community met to discuss the current status of ad intervention trials and future plans for designing trials of prodromal ad and/or mild cognitive impairment. there is no agreement regarding a single pathogenic mechanism to be targeted, although a consensus seems to be emerging that effective treatment will require attacking multiple targets. thes ... | 2009 | 19118811 |
| characterization of anaplasma infections in sicily, italy. | this study aimed to characterize infection with anaplasma marginale, a. phagocytophilum, a. ovis, and a. platys in humans, animals, and ticks in sicily, italy, during 2003-2006. serologic (competitive elisa [celisa]) and indirect immunofluorescence antibody [ifa]; n= 1990) and dna (polymerase chain reaction [pcr]; n= 2788) tests were conducted on horse, donkey, cattle, sheep, goat, pig, dog, cat, roe deer, wild boar, human, and tick samples. the results reported herein suggested that in sicily c ... | 2008 | 19120181 |
| eutrophication of lake tasaul, romania-proposals for rehabilitation. | lake tasaul on the black sea coast is highly eutrophic, but not strongly contaminated (heavy metals, pahs, and organochlorine pesticides). cyanophytes dominate phytoplankton by 67-94% and form frequent algal blooms. high primary production (up to 270 mg c(ass)/m(2).h) and algal biomass (maximum chlorophyll a concentration 417 microg/l) may be controlled by light, as secchi depth is often below 1 m. the main tributary, casimcea river, provides high quantities of suspended matter and about 3 tons ... | 2009 | 19132430 |
| recombinant human arginase inhibits proliferation of human hepatocellular carcinoma by inducing cell cycle arrest. | human hepatocellular carcinoma (hcc) has an elevated requirement for arginine in vitro, and pegylated recombinant human arginase i (rharg-peg), an arginine-depleting enzyme, can inhibit the growth of arginine-dependent tumors. while supplementation of the culture medium with ornithine failed to rescue hep3b cells from growth inhibition induced by rharg-peg, citrulline successfully restored cell growth. the data support the roles previously proposed for ornithine transcarbamylase (otc) in the arg ... | 2009 | 19138817 |
| studies on besnoitiosis bennetti in miniature donkeys. | besnoitia tissue cysts associated with the skin lesions recovered from the naturally-infected miniature donkeys (equus asinus) during clinical examination were studied by the light and electron microscopy, as well as histochemically to elucidate the specific morphologic features of the cyst causing this disease. the cyst was differentiated phenotypically from those of other besnoitia spp. the interpretation of results showed that morphometric attributes of the tissue cysts and the associated pat ... | 2008 | 19143129 |
| zoonotic hydatidosis in donkeys: post mortum examination in the zoo, giza, egypt. | a total of 17 out of 160 donkeys slaughtered had hepatic hydatidosis as proved macroscopically and microscopically. the epidemiology of zoonotic echinococcosis/hydatidosis was reviewed and discussed. | 2008 | 19143140 |
| evaluation of plasma membrane integrity of donkey spermatozoa. | the aim of the study was to develop a hypo-osmotic swelling test (hos-test) for evaluating plasma membrane integrity in donkey spermatozoa. in the first study, six different hypo-osmotic solutions (fructose or fructose/sodium citrate, 75 or 150 mosm, or bi-distilled water at 1 : 10 semen : solution ratio, or bi-distilled water at 1 : 3) were compared. the 75 mosm fructose solution (1 : 10) and bi-distilled water (1 : 3) were chosen for study 2, where two incubation times (5 or 45 min) were teste ... | 2010 | 19144042 |
| arginine deiminase as a novel therapy for prostate cancer induces autophagy and caspase-independent apoptosis. | arginine deprivation as an anticancer therapy has historically been met with limited success. the development of pegylated arginine deiminase (adi-peg20) has renewed interest in arginine deprivation for the treatment of some cancers. the efficacy of adi-peg20 is directly correlated with argininosuccinate synthetase (ass) deficiency. cwr22rv1 prostate cancer cells do not express ass, the rate-limiting enzyme in arginine synthesis, and are susceptible to adi-peg20 in vitro. interestingly, apoptosi ... | 2009 | 19147587 |
| concurrent unilateral choanal atresia and congenital laryngeal deformity in a miniature donkey. | | 2009 | 19151409 |
| adult unilateral supraglottitis as a consequence of acute submandibular sialadentis. | acute submandibular sialadenitis (ass) may spread to sublingual and submandibular spaces. on rare occasions it can spread along the floor of the mouth, to the base of the tongue and to the pre epiglottic space, valleculae and epiglottis, leading to supraglottitis. adult supraglottitis is an acute inflammation of the supraglottic structures. unilateral supraglottitis in an adult as a consequence of ass is a unique complication as described in this case report. sore throat with severe odynophagia ... | 2008 | 19157331 |
| toxic effects of two acid sulfate soils from the dabaoshan mine on corymbia citriodora var.variegata and daphnia carinata. | acidic, metal-stressed conditions encountered in the acid sulfate soils significantly inhibited the growth of corymbia citriodora var.variegata, possibly due to the reduced rate of photosynthesis and plant root activity. however, the plant's self-protection mechanism to counteract stress-induced cellular damage by reactive oxygen species still functioned well even at a soil ph as low as 2.81. this may explain the high tolerance of this plant species to the extremely acidic environments. the obse ... | 2009 | 19157696 |
| development of a highly sensitive elisa for aldosterone in mouse urine: validation in physiological and pathophysiological states of aldosterone excess and depletion. | clinical studies have established aldosterone as a critical physiological and pathophysiological factor in salt and water homeostasis, blood pressure control and in heart failure. genetic and physiological studies of mice are used to model these processes. a sensitive and specific assay for aldosterone is therefore needed to monitor adrenocortical activity in murine studies of renal function and cardiovascular diseases. | 2009 | 19162057 |
| optimized shift-invariant wavelet packet feature extraction for electroencephalographic evoked responses. | local discriminant bases (ldb) have a major disadvantage in their representation which is sensitive to signal translations. the discriminant features will be not consistent when the same but shifted signal is applied. thus, to overcome this problem, an approximate shift-invariant features extraction based on local discriminant bases is introduced. this technique is based on approximate shift-invariant wavelet packed decomposition which integrate a cost function for decimation decision in each su ... | 2008 | 19163258 |
| noninvasive estimation of three-dimensional cardiac electrical activities from body surface potential maps. | a noninvasive three-dimensional (3d) cardiac electrical imaging (3dcei) approach, which can estimate the location of the initiation site (is) of activation and the resultant 3d activation sequence (as) from body surface potential maps (bspms), was validated in an intact large mammalian model (swine) during acute ventricular pacing. body surface potential mapping and intracavitary noncontact mapping (ncm) were performed simultaneously during pacing from both right ventricular (rv) sites (intramur ... | 2008 | 19163726 |
| quality of care of patients with acute myocardial infarction in bulgaria: a cross-sectional study. | cardiovascular diseases are the major cause of death in bulgaria. because of notable differences in mortality rates between bulgaria and other european countries, we presume a tangible difference in the management of acute myocardial infarction (ami) and an underutilization of evidence-based treatments. in order to determine the quality of care of patients with ami in bulgaria, we analyzed the appropriateness of current treatments and their relation to patient characteristics. | 2009 | 19171057 |
| palliative effect of lafutidine on oral burning sensation. | lafutidine is a unique histamine h(2)-receptor antagonist (h2ra) that has a sensitizing effect on capsaicin-sensitive afferent neurons (csan). this effect may make lafutidine useful for the treatment of burning mouth syndrome (bms). | 2009 | 19175712 |
| the morphology of the cervico-thoracic sympathetic system in donkeys (equus asinus l.). | five adult donkeys of both sexes, used in applied anatomy classes, and perfused with formalin for teaching purposes, constituted the study material. ganglion cervicale caudale of the examined materials has observed to exhibit individually variable situation as to extend on the left side of the median line, at the alignment of the first and second intercostal spaces and on the right side between the level of the first and third costa. the ganglion extended more caudally on the right side of media ... | 2009 | 19183351 |
| structures of the ceramides from porcine palatal stratum corneum. | ceramides are the major type of lipid found in stratum corneum from the skin, gingiva and hard palate. the present study examined the ceramides of the stratum corneum from the hard palate. six fractions of ceramides were isolated by preparative thin-layer chromatography. the least polar fraction contained an unusual acyl ceramide (eos) consisting of long omega-hydroxy acids amide-linked to sphingosine with mostly saturated fatty acids ester-linked to the omega-hydroxyl group. the second and thir ... | 2009 | 19184160 |
| parental genomes mix in mule and human cell nuclei. | whether chromosome sets inherited from father and mother occupy separate spaces in the cell nucleus is a question first asked over 110 years ago. recently, the nuclear organization of the genome has come increasingly into focus as an important level of epigenetic regulation. in this context, it is indispensable to know whether or not parental genomes are spatially separated. genome separation had been demonstrated for plant hybrids and for the early mammalian embryo. conclusive studies for somat ... | 2009 | 19198867 |
| highly selective monomethylation of primary amines through host-guest product sequestration. | this communication reports the highly selective monomethylation of primary amines through host-guest product sequestration. complete control of the outcome of the n-methylation reaction has been achieved by adding to the reaction medium stoichiometric amounts of a teraphosphonate phosphonate cavitand tiiii, capable of selectively and quantitatively trapping the monomethylated ammonium salt formed. the synergistic combination of ion-dipole, h-bonding, and ch(3)-pi interactions provide the high as ... | 2009 | 19199632 |
| phylogenetic analysis and arsenate reduction effect of the arsenic-reducing bacteria enriched from contaminated soils at an abandoned smelter site. | microbial reduction of as(v) (i.e., arsenate) plays an important role in arsenic (as) mobilization in aqueous environment. in this study, we investigated as(v) reduction characteristics of the bacteria enriched from the arsenic-contaminated soil at an abandoned smelter site. it was found that as(v) was completely reduced to as(iii) (i.e., arsenite) in 21 h. after 3-d incubation, a yellow solid was precipitated and the concentration of as(iii) decreased sharply. after 150 h incubation, ca. 65% of ... | 2008 | 19209639 |
| an update review on commiphora molmol and related species. | the origins of myrrh and frankincense are traced to the arabian peninsula. according to herodotus (5th century bc): "arabia is the only country which produces frankincense, myrrh, cassia, and cinnamon.., the trees bearing the frankincense are guarded by winged serpents of small size and various colors." diodorus siculus wrote, in the second half of the first century bc, that "all of arabia exudes a most delicate fragrance; even the seamen passing by arabia can smell the strong fragrance that giv ... | 2008 | 19209761 |
| evaluation of the seminiferous epithelial cycle, spermatogonial kinetics and niche in donkeys (equus asinus). | kinetics of spermatogonia as well as localization in niches have been described in rodents, but rarely in large animals or in species of economical interest. in this regard, and envisioning the possibility of spermatogonial transplantation from donkeys (equus asinus) to mules (equus mulus mulus), many variables that may contribute for an enhanced understanding of the spermatogonial biology in donkeys were investigated. testes from five adult donkeys were routinely processed for high-resolution l ... | 2009 | 19211205 |
| in situ fluorescence microscopic investigation into the dependence of conformation and electrophoretic velocity of single dna molecules on acid or spermidine concentration. | a measurement cell for dna migration was fabricated by agarose gel on a glass plate for the purpose of in situ fluorescence microscopic measurements of the electrophoretic behaviors of single giant dna (t4gt7dna, 165600 base pairs) molecules. the cell was connected to an original circuit that can supply a constant electric field strength to a dna-migration-space of the cell. conformation and electrophoretic velocity of single dna molecules were measured at the same time as a function of acid or ... | 2009 | 19212068 |
| the evaluation of interictal focal eeg findings in adult patients with absence seizures. | to investigate the focal interictal eeg abnormalities in adult patients with absence seizures (ass) and to identify their clinical, eeg and semiological correlates. | 2009 | 19213578 |
| evaluation of endogenous nitric oxide synthesis in congenital urea cycle enzyme defects. | nitric oxide (no) is synthesized from arginine and o(2) by nitric oxide synthase (nos). citrulline, which is formed as a by-product of the nos reaction, can be recycled to arginine by the 2 enzymes acting in the urea cycle: argininosuccinate synthetase (ass) and argininosuccinate lyase (asl). although the complete urea cycle is expressed only in the liver, ass and asl are expressed in other organs including the kidney and vascular endothelium. to examine possible alterations of the no pathway in ... | 2009 | 19217439 |
| [comparison of proviral genomes between the chinese eiav donkey leukocyte-attenuated vaccine and its parental virulent strain]. | the donkey leukocyte-attenuated vaccine of equine infectious anemia virus (eiav) was the first lentiviral vaccine that induced solid protection from the infection of virulent strains. to elucidate the mechanism of increased immunogenicity and attenuated virulence of the vaccine, the proviral genomic dna of an eiav vaccine strain, eiav(dlv121) was analyzed and compared with the genome of a parental virulent strain eiav(dv117). full length viral genomic dnas were amplified as two segments by la-pc ... | 2008 | 19226953 |
| an xps analytical approach for elucidating the microbially mediated enargite oxidative dissolution. | in this work, the microbe-mediated oxidative dissolution of enargite surfaces (cu(3)ass(4)) was studied on powdered samples exposed to 9k nutrient solution (ph 2.3) inoculated by acidithiobacillus ferrooxidans initially adapted to arsenopyrite. these conditions simulate the acid mine environment. the redox potential of the inoculated solutions increased up to +0.72 v vs normal hydrogen electrode (nhe), indicating the increase of the fe(3+) to fe(2+) ratio, and correspondingly the ph decreased to ... | 2009 | 19229526 |