| the g-protein g(o) in mammalian cardiac muscle: localization and coupling to a1 adenosine receptors. | to investigate the functional relevance of the g-protein g(o) to adenosine-induced effects in the heart, we studied the localization of g(o) and its interaction with a1 adenosine receptors. concentrations of g(o) in various mammalian hearts differed markedly between the atrial and the ventricular muscle, as well as among species. in most species examined, the concentration of g(o) was much higher in the atrium than in the ventricle. the highest levels of g(o) in atria and ventricles were found i ... | 1995 | 7775387 |
| synaptic integration in layer iv of the ferret striate cortex. | 1. whole-cell patch recording were made with dye-filled electrodes from layer iv in slices of the ferret striate cortex. projections from the thalamus and layer vi provide most of the extralaminar input to layer iv. interactions between these two pathways are thought to play a role in the generation of suppressive non-linearities such as end-inhibition. thus, synaptic responses evoked by stimulating each pathway individually were compared with those produced by activating both projections togeth ... | 1995 | 7776231 |
| synaptic and membrane mechanisms underlying synchronized oscillations in the ferret lateral geniculate nucleus in vitro. | 1. the cellular basis for generation of spindle waves and a slower synchronized oscillation resembling absence seizures was investigated with extracellular and intracellular recording techniques in slices of ferret dorsal lateral geniculate nucleus (lgnd) maintained in vitro. 2. intracellular recording from lgnd relay cells in vitro revealed that spindle waves occurred once every 3-30 s and were associated with barrages of inhibitory postsynaptic potentials (ipsps) occurring at a frequency of 6- ... | 1995 | 7776249 |
| role of the ferret perigeniculate nucleus in the generation of synchronized oscillations in vitro. | 1. the cellular mechanisms by which neurons of the ferret perigeniculate nucleus (pgn) participate in the generation of spindle waves and slowed absence seizure-like oscillations were investigated with intracellular and extracellular recording techniques in geniculate slices maintained in vitro. 2. during spindle wave generation, pgn neurons generated repetitive (2-9 hz) high frequency (up to 500 hz) burst discharges mediated by the activation of a low threshold ca2+ spike by the arrival of barr ... | 1995 | 7776250 |
| acetylcholinesterase in the developing ferret retina. | the function of acetylcholinesterase (ache) is to terminate the action of acetylcholine at the cholinergic synapse. recent evidence suggests additional roles for acetylcholinesterase as a peptidase and/or a protease which is expressed by growing neurites as part of their invasion of developing neural structures. we report the localization of acetylcholinesterase in developing ferret retina. ache histochemical staining is seen in the developing inner plexiform layer (ipl) of ferret retina at birt ... | 1995 | 7781740 |
| protein hpn: cloning and characterization of a histidine-rich metal-binding polypeptide in helicobacter pylori and helicobacter mustelae. | helicobacter pylori is a human gastrointestinal pathogen involved in gastritis, duodenal ulcers, and gastric neoplasia. this microorganism produces large amounts of a urease which, like all known ureases, has nickel in the active site. we have identified a protein in clinical isolates of h. pylori and an identical protein in the ferret pathogen helicobacter mustelae that strongly binds ni2+ and zn2+. this protein has been named hpn to emphasize its origins in h. pylori and its affinity for nicke ... | 1995 | 7790085 |
| immunolocalisation of protein phosphatase inhibitor-1 in the cerebral cortex of the rat, cat and ferret. | the distribution of inhibitor-1 was analysed in the neocortex of cat, ferret and rat by immunocytochemistry (at the light and electron microscope levels) and by immunoblotting using an affinity purified antibody which recognises both the phosphorylated and dephosphorylated forms of the protein. in each mammalian cortex immunocytochemical techniques identified inhibitor-1 predominantly in infragranular pyramidal neurons and, at a lower concentration, in supragranular pyramidal neurons of cortical ... | 1995 | 7796181 |
| cardiac electrophysiologic and antiarrhythmic actions of two long-acting spirobenzopyran piperidine class iii agents, l-702,958 and l-706,000 [mk-499]. | the cardiac electrophysiologic and antiarrhythmic actions of two class iii ketone- and alcohol-containing spirobenzopyran piperidine analogs, l-702,958 and l-706,000 [mk-499], respectively, were assessed in vitro and in vivo. l-702,958 and l-706,000 [mk-499] selectively blocked the rapidly activating component of the delayed rectifier k+ current in guinea pig isolated ventricular myocytes (ic50 values, 14.6 and 43.9 nm, respectively), and prolonged effective refractory period in ferret isolated ... | 1994 | 7802864 |
| identification of two genetically distinct strains of pneumocystis carinii in infected ferret lungs. | | 1994 | 7804271 |
| production and characterization of monoclonal antibodies against guinea pig tracheal mucins. | thirty-five hybridomas that secrete mouse monoclonal antibodies (mab) against guinea pig (g.p.) tracheal mucins were established. the mabs were characterized immunologically, biochemically, and immunohistochemically at both light and electron microscopic levels. isotyping of the mabs revealed 14 to be igm, 13 igg1, 3 igg2, and 5 igg3. the mabs demonstrated various patterns of binding in immunoblots against mucins derived from g.p. tracheal explants. this suggested the presence of "subpopulations ... | 1994 | 7806248 |
| resistance and disease in brugia malayi infection of ferrets following prior infection, injection of attenuated infective larvae and injections of larval extracts. | a partial resistance expressed by a 53% to 78% reduction in lymphatic filariae from a challenge infection was induced in ferrets (mustela putorius furo) by a prior infection and by injection of radiation attenuated infective larvae (l3) but not by injections of lyophilized microfilariae (mf) or l3. equivalent acquired resistance was demonstrated with and without overt filarial disease. a prior infection resulted in peripheral lymphoedema in approximately one-third of the amicrofilaraemic resista ... | 1994 | 7808763 |
| evolution of alanine:glyoxylate aminotransferase 1 peroxisomal and mitochondrial targeting. a survey of its subcellular distribution in the livers of various representatives of the classes mammalia, aves and amphibia. | as part of a wider study on the molecular evolution of alanine:glyoxylate aminotransferase 1 (agt1) intracellular compartmentalization, we have determined the subcellular distribution of immunoreactive agt1, using postembedding protein a-gold immunoelectron microscopy, in the livers of various members of the classes mammalia, aves, and amphibia. as far as organellar distribution is concerned, three categories could be distinguished. in members of the first category (type i), all, or nearly all, ... | 1994 | 7813517 |
| profiles of emetic action of cisplatin in the ferret: a potential model of acute and delayed emesis. | the emetic profile of action of cisplatin 5 and 10 mg/kg i.p. was investigated in the ferret over a 72 and 40 h period respectively. cisplatin 10 mg/kg induced retching and vomiting which rapidly declined after the first 8 h. cisplatin 5 mg/kg induced a less intense emetic response which declined after 16 h but reappeared at approximately 32 h to reveal a 'delayed' retching and vomiting response. the use of cisplatin 5 mg/kg may offer a regimen to model cytotoxic acute and delayed emesis. | 1994 | 7813558 |
| the effect of nerve injury on the incidence and distribution of branched pulpal axons in the ferret. | in a previous electrophysiological study in ferrets, we demonstrated that some axons in the inferior alveolar nerve branch to supply the pulps of two teeth. we have now investigated the incidence and distribution of branched pulpal axons at various intervals after nerve injury and subsequent regeneration, to study the extent to which the innervation of the teeth returns to normal. in adult male ferrets under anesthesia, the left inferior alveolar nerve was either sectioned (31 animals) or crushe ... | 1994 | 7814751 |
| effects of adenosine on ca2+ transients and tension in aequorin-injected ferret papillary muscles. | the effects of adenosine on ca2+ transients and tension in ferret papillary muscles were investigated using the aequorin method. adenosine (0.01-1 mm) reduced the peak of ca2+ transients and caused a slight concentration-dependent decrease in tension. adenosine prolonged the decay time of ca2+ transients but did not alter the time course of tension. in isoproterenol (0.1 microm)-treated preparations, adenosine decreased the peak of ca2+ transients but did not alter the peak of tension. adenosine ... | 1994 | 7816558 |
| an n-methyl-d-aspartate receptor antagonist does not prevent eye-specific segregation in the ferret retinogeniculate pathway. | recent studies have shown that electrical activity, particularly that mediated by nmda receptors, has a profound effect on the development of specific neuronal connections. blocking nmda receptors in the ferret's lateral geniculate nucleus prevents the segregation of retinal afferents into on and off sublaminae. we have now examined the involvement of nmda receptors in the separation of afferents from the two eyes that occurs in the lateral geniculate nucleus several weeks earlier in development ... | 1994 | 7834339 |
| effect of cyclopiazonic acid on contractile responses in slow and fast bundles of cremaster skeletal muscle from the ferret. | the effects of cyclopiazonic acid (cpa) on twitch force, calcium (ca2+) uptake and release by the sarcoplasmic reticulum (sr), and ca2+ sensitivity of contractile apparatus were studied using intact and chemically skinned cremaster fibers and compared with those on the extensor digitorum longus and soleus. in cremaster muscles treated with cpa (0.5-5 microm) a potentiation of the twitch was observed, associated with an increase in time to peak and in time of relaxation. in triton-skinned fibers, ... | 1994 | 7834571 |
| permeability of ferret trachea in vitro to 99mtc-dtpa and [14c]antipyrine. | platelet-activating factor (paf) and vasoactive drugs were tested on permeability of ferret trachea in vitro by measuring fluxes of 99mtc-diethylenetriamine pentaacetic acid (99mtc-dtpa; hydrophilic) and [14c]antipyrine ([14c]ap; lipophilic) across the tracheal wall. tracheae were bathed on both sides with krebs-henseleit buffer, with luminal buffer containing either 99mtc-dtpa or [14c]ap. luminal and abluminal radioactivities, potential difference, and tracheal smooth muscle tone were measured. ... | 1994 | 7836130 |
| modulation of myocardial relaxation by basal release of endothelin from endocardial endothelium. | the aim was to study the potential role of basal endothelin-1 release from endocardial endothelium in isolated ferret papillary muscle preparations. | 1994 | 7842464 |
| development of muscarinic acetylcholine receptors in the ferret retina. | the development of muscarinic acetylcholine receptor protein in the ferret retina was studied using biochemical, autoradiographic, and light and electron microscopic immunohistochemical techniques. the development of retinal muscarinic cholinergic receptor proteins involves transient shifts in their number and distribution, as well as changes in the relative abundance of two molecular weight variants. receptor binding assays demonstrate changes in the number and affinity of retinal binding sites ... | 1994 | 7842519 |
| evidence for cholinergic vagal afferents and vagal presynaptic m1 receptors in the ferret. | the distribution of muscarinic receptor binding was examined in the ferret brainstem vagal nuclei using the non-selective ligand [3h]quinuclidinyl benzilate and the relatively m1 receptor-selective ligand [3h]pirenzepine. the highest density of receptor sites are found in the subnucleus gelatinosus and lower levels in the other subnuclei of the nucleus of the tractus solitarius and in the area postrema and dorsal motor nucleus of the vagus nerve. dense binding was also seen in the adjacent hypog ... | 1994 | 7849574 |
| thermoregulatory effects of resiniferatoxin in the rat. | when administered acutely, the vanilloid (capsaicin) receptor agonist resiniferatoxin induces marked hypothermia in the ferret, rat and mouse. the aim of this study was to further characterise the thermoregulatory effects of resiniferatoxin in the rat in an attempt to understand the mechanism by which resiniferatoxin induces this hypothermic effect. three doses of resiniferatoxin were administered (50, 100, 200 micrograms/kg s.c.) in separate animals at an ambient temperature (ta) of 20 degrees ... | 1994 | 7851474 |
| ultrasonographic detection of adrenal gland tumors in two ferrets. | hyperadrenocorticism is recognized as a common medical condition in middle- to old-aged ferrets. because diagnosis of adrenocortical tumors in ferrets, using results of adrenocorticotropic hormone stimulation or dexamethasone suppression tests, is unreliable, ultrasonography was used as an alternative to laparotomy for the detection of tumors in 2 ferrets. the diagnosis was confirmed by surgical excision and histologic examination of the adrenal gland. in a typical manifestation of the condition ... | 1994 | 7852151 |
| morphology of retinal axons induced to arborize in a novel target, the medial geniculate nucleus. i. comparison with arbors in normal targets. | ferret retinal axons can be induced to innervate the medial geniculate nucleus (mgn) by a combination of brain lesions early in development. our previous work suggests that the retinal ganglion cells responsible for this plasticity are w cells. the present study continues this work with a morphological investigation of normal retinal ganglion-cell axons and retinal ganglion-cell axons induced to arborize in the mgn. retinal axons were bulk filled with horseradish peroxidase placed in the optic t ... | 1994 | 7852629 |
| differential effects of the serotonin1a agonist, 8-oh-dpat, on masculine and feminine sexual behavior of the ferret. | administration of the serotonin (5-ht)1a receptor agonist 8-hydroxy-2-(di-n-propylamino)tetralin (8-oh-dpat) facilitates the expression of masculine sexual behavior in male and female rats as well as in male rhesus monkeys and inhibits lordosis behavior in female rats. in the present study the effects of 8-oh-dpat on masculine coital and feminine proceptive and receptive behaviors were evaluated in the ferret, a carnivore. doses of 8-oh-dpat (0.1 or 0.2 mg/kg) that facilitate masculine sexual be ... | 1994 | 7855220 |
| mechanism of the negative inotropic effect of thiopental in isolated ferret ventricular myocardium. | thiopental's myocardial depressant effects are well known and most likely involve some alteration in intracellular ca2+ homeostasis. the aim of this study was to investigate the mechanisms of thiopental's negative inotropic effects and its underlying mechanism in isolated ferret ventricular myocardium (which shows physiologic characteristics similar to human ventricular myocardium), and in frog ventricular myocardium, in which ca2+ ions for myofibrillar activation are derived almost entirely fro ... | 1995 | 7856902 |
| glial domains and axonal reordering in the chiasmatic region of the developing ferret. | this study has examined the developing glial architecture of the optic pathway and has related this to the changing organization of the constituent axons. immunocytochemistry was used to reveal the distribution of glial profiles, and dii was used to label either radial glial profiles or optic axons. electron microscopy was used to determine the distribution of glial profiles, axons, growth cones, and wrists at different locations along the pathway. three different glial boundaries were defined: ... | 1994 | 7860785 |
| nmda-receptor antagonists disrupt the formation of the auditory space map in the mammalian superior colliculus. | in the ferret (mustela putorius) the map of auditory space in the deeper layers of the superior colliculus (sc) matures over a period of several postnatal weeks, a process known to be guided by both visual and auditory experience. the auditory responses are initially very broadly tuned, and gradually become more selective for specific sound locations that coincide with the visual receptive fields recorded in the same region of the sc. to investigate the possible involvement of nmda-type glutamat ... | 1995 | 7869115 |
| the role of the sarcoplasmic reticulum in the response of isolated ferret cardiac muscle to beta-adrenergic stimulation. | beta-adrenergic stimulation of cardiac muscle leads to an increase in the strength of contraction and an abbreviation of its time course. we have investigated the role of the sarcoplasmic reticulum in these changes by monitoring force and cytoplasmic [ca2+] in ferret papillary muscles, and the ca2+ current in isolated ferret myocytes, during the application of isoprenaline in the absence and presence of the sarcoplasmic reticulum inhibitor ryanodine (10(-6) mol/l). isoprenaline (10(-6) mol/l) le ... | 1994 | 7873161 |
| ultrastructural evidence for synaptic interactions between thalamocortical axons and subplate neurons. | thalamic axons are known to accumulate in the subplate for a protracted period prior to invading the cortical plate and contacting their ultimate targets, the neurons of layer 4. we have examined the synaptic contacts made by visual and somatosensory thalamic axons during the transition period in which axons begin to leave the subplate and invade the cortical plate in the ferret. we first determined when geniculocortical axons leave the subplate and begin to grow into layer 4 of the visual corte ... | 1994 | 7874312 |
| vitamin e enhances the lymphatic transport of beta-carotene and its conversion to vitamin a in the ferret. | beta-carotene and alpha-tocopherol may have either antagonistic or synergistic effects on each other's absorption and metabolism. the effects of both physiological and pharmacological concentrations of alpha-tocopherol on the absorption and metabolism of beta-carotene in ferret intestine were determined. | 1995 | 7875474 |
| protection of ferrets against influenza challenge with a dna vaccine to the haemagglutinin. | immunization of ferrets with a plasmid dna expressing influenza virus haemagglutinin (pcmv/h1 dna) provided complete protection from challenge with the homologous a/pr/8/34 (h1n1) influenza virus. delivery of dna-coated gold beads by gene gun to the epidermis was much more efficient than intramuscular delivery of dna in aqueous solution. the antibody response induced by dna delivered by gene gun was more cross-reactive than dna delivered in aqueous solution or after natural infection. this novel ... | 1994 | 7879412 |
| species-related differences in inotropic effects of angiotensin ii in mammalian ventricular muscle: receptors, subtypes and phosphoinositide hydrolysis. | 1. experiments were carried out to clarify the mechanisms responsible for variations in the positive inotropic effect (pie) of angiotensin ii (aii) on ventricular muscles from various mammals. we examined the density of aii receptors, the relative proportions of receptor subtypes and the acceleration of the hydrolysis of phosphoinositide that was induced by aii, as well as the pie of aii in ventricular muscles from the rabbit, dog, rat and ferret. 2. in the rabbit, aii (1 microm) in the presence ... | 1995 | 7881743 |
| 'sensory-efferent' neural control of mucus secretion: characterization using tachykinin receptor antagonists in ferret trachea in vitro. | 1. we characterized the tachykinin receptor(s) mediating 'sensory-efferent' neural control of release of 35so4-labelled macromolecules (mucus) from ferret trachea in vitro in ussing chambers using selective tachykinin antagonists. secretion was induced by substance p (sp), neurokinin a (nka), capsaicin, the nk1 tachykinin receptor agonist [sar9, met(o2)11]substance p ([sar9]sp), or acetylcholine (ach), or by electrical stimulation of nerves. antagonists used were fk888 and l-668,169, selective f ... | 1994 | 7889271 |
| copper toxicosis in sibling ferrets. | two sexually intact female silver-shaded domestic ferret siblings from different litters were examined because of cns depression and lethargy. ferret 1 was dehydrated and hypothermic, whereas ferret 2 was icteric and febrile and had serum bilirubin concentration > 12.0 mg/dl and bun of 59 mg/dl. despite supportive treatment, the ferrets died within days of evaluation. on necropsy, ferret 1 had chronic hepatopathy, with diffuse vacuolation of hepatocytes. in ferret 2, the liver had centrilobular ... | 1994 | 7890574 |
| subcellular calcium pools of ischaemic and reperfused myocardium characterised by electron probe. | the subcellular redistribution of calcium and other electrolytes was analysed in myocardium subjected to global ischaemia and reperfusion in order to establish possible causes of reperfusion injury. | 1995 | 7895244 |
| ionic basis of the electrophysiological actions of adenosine on cardiomyocytes. | the purpose of this review is to examine the role of the extracellular a1-adenosine (ado) receptor in modulating membrane potential and currents in cardiac cells. the cellular electrophysiological effects of adenosine are both cell type- and species-dependent. in supraventricular tissues (sa, av node, and atrium) of all species studied, the "direct" camp-independent activation of the inwardly rectifying k+ current ikado seems to be the most important action of adenosine. this current is activate ... | 1995 | 7896004 |
| complex ceruminous gland adenocarcinoma in a brown-footed ferret (mustela putorius furo). | | 1994 | 7898040 |
| myocardial salvage efficacy of the thromboxane receptor antagonist ifetroban in ferrets and dogs. | the effects of the thromboxane a2 (txa2)/prostaglandin endoperoxide (tp) receptor antagonist ifetroban (bms-180291) on infarct size (is) resulting from coronary occlusion/reperfusion was determined in anesthetized dogs and ferrets. in dogs, ifetroban (1 + 1 mg/kg/h, intravenously, i.v.) or vehicle administration was initiated 10 min before left circumflex coronary artery (lcx) occlusion. in ferrets, the left anterior descending coronary artery (lad) was occluded; after 75 min, a continuous infus ... | 1994 | 7898080 |
| protein kinase inhibitor h-89 reverses forskolin stimulation of cardiac l-type calcium current. | calcium currents (ica) and barium currents (iba) were measured in freshly isolated single ferret ventricular myocytes, using the whole cell patch-clamp and perforated patch-clamp techniques with na and k currents blocked by tetraethylammonium and cs. the membrane potential (em) dependence of activation and steady-state inactivation curves were determined using a boltzmann relation, where e0.5 is the em at half-maximal conductance. forskolin (1 microm) increased the rate of ica inactivation, espe ... | 1995 | 7900772 |
| neuromodulation of calcium current by extracellular atp in isolated ventricular myocytes. | the effects of extracellular atp on the l-tpe ca2+ current (ica), action potential, and resting and intracellular ca2+ levels were examined in enzymatically isolated myocytes from the right ventricles of ferrets. extracellular atp decreased the peak amplitude of ica in a time- and concentration-dependent manner. the concentration-response relationship for atp inhibition of ica was well described by a conventional michaelis-menten relationship with a half maximal inhibitory concentration of 1 mic ... | 1993 | 7910429 |
| rearrangements of synaptic connections in visual cortex revealed by laser photostimulation. | assessing patterns of synaptic connections in the developing mammalian neocortex has relied primarily on anatomical studies. in a physiological approach described here, the patterns of synaptic connections in slices of developing ferret visual cortex were determined with scanning laser photostimulation. functional synaptic inputs to pyramidal cells in cortical layers 2 and 3 originating from sites close to the neuronal cell body appeared at least 2 weeks before eye opening, prior to the formatio ... | 1994 | 7912852 |
| the pathogenesis of crohn's disease. | this paper describes a program of research undertaken by the inflammatory bowel disease study group at the royal free hospital school of medicine. the group has tested the hypothesis that the primary pathological abnormality in crohn's disease is in the mesenteric blood supply. the first experiments involved microcorrosion resin casting of the arterial supply of specimens of resected intestine affected by crohn's disease. this revealed severe damage to submucosal blood vessels, even in areas tha ... | 1994 | 7921140 |
| terminal types on ipsilaterally and contralaterally projecting lateral superior olive cells. | the lateral superior olive (lso) in ferret contains two distinct populations of principal cells, one population projecting to the ipsilateral and the other projecting to the contralateral inferior colliculus. in addition, these populations have been shown to be distinct from each other on the basis of tonotopic and isofrequency distribution within lso, of dendritic morphology, and of neurotransmitter within the somata. the present study compared the two populations on the basis of the type of sy ... | 1994 | 7928743 |
| potentiation of infectivity and pathogenesis of influenza a virus by a house dust mite protease. | common house dust mites (e.g., dermatophagoides farinae) excrete a serine-type (df) protease. df protease obtained from cultured mites enhanced viral replication in vitro via proteolytic cleavage of viral hemagglutinin (ha) into ha1 and ha2, which confers potent viral infectivity. its potency is 2- to 5-fold higher than bovine trypsin or human plasmin. df protease also markedly accelerated virus propagation in vivo: a minute quantity of protease (estimated delivered amount, 0.8-3.2 micrograms) p ... | 1994 | 7930699 |
| role of surface glycoproteins in influenza virus pyrogenicity. | eleven h3n2, seven h1n1 and three h3n1 influenza virus reassortants of the pyrogenic a/puerto rico/8/34-a/england/939/69 clone 7a (h3n2) (a/7a) and poorly pyrogenic a/fiji/15899/83 (h1n1) (a/fiji) parents were analysed genetically for the parental origin of their genes and for their pyrogenicity in ferrets. all h3n2 reassortants were pyrogenic and produced significantly more fever than a/fiji but differences in pyrogenicity between them could not be correlated with either single or constellation ... | 1994 | 7931175 |
| nonpeptide renin inhibitors with good intraduodenal bioavailability and efficacy in dog. | the aim of this study was the discovery of nonpeptide renin inhibitors with much improved oral absorption, bioavailability, and efficacy, for use as antihypertensive agents. our prior efforts led to the identification of a-74273 [1,r = 3-(4-morpholino)propyl], with a bioavailability of 26 +/- 10% [10 mg/kg intraduodenally (id), dog]. in vivo metabolism studies of a-74273 showed that the morpholino moiety underwent metabolic degradation. computer modeling of a-74273 bound to renin indicated that ... | 1994 | 7932521 |
| cloning and characterization of an ito-like potassium channel from ferret ventricle. | fk1, a ferret ventricular full-length cdna clone, encodes a 654-amino acid protein with 98% identity to human k+ transient outward current (ito)-like hk1 (tamkun et al. faseb j.5: 331-337, 1991). fk1 is detectable in ferret brain, atrium, left and right ventricle, and kidney but not in skeletal muscle, endothelial cells, aorta, and liver. in xenopus oocytes, fk1 crna gives rise to a rapidly activating and inactivating ito-like current, which is highly k+ selective (na(+)-to-k+ permeability ratio ... | 1994 | 7943383 |
| a histological comparison of periapical inflammatory and neural responses to two endodontic sealers in the ferret. | previous studies have shown that there is a proliferation of nerves beneath the apices of pulpectomized teeth. this may be due to the inflammation induced after the procedure, resulting, perhaps, from the irritant nature of the materials used to fill the root canal. the experiment reported here was conducted to determine whether this inflammation was induced by the sealer rather than arising as a result of tissue damage and whether, if inflammation is eliminated or reduced, the neural changes ar ... | 1994 | 7945012 |
| effects of acidosis and hypoxia on the response of isolated ferret cardiac muscle to inotropic agents. | the aim was to study the effects of acidosis and hypoxia on the response of cardiac muscle to inotropic agents which (a) act predominantly by increasing intracellular [ca2+] (raising extracellular [ca2+], noradrenaline, isoprenaline) and (b) act partly (phenylephrine) or predominantly (emd 57033) by increasing myofilament calcium sensitivity. | 1994 | 7954624 |
| megaesophagus in nine ferrets. | megaesophagus was diagnosed in 9 adult ferrets. clinical history of the ferrets included regurgitation, difficulty in swallowing, partial anorexia, and lethargy. cachexia, dehydration, weakness, and ptyalism were observed on physical examination. radiography revealed the esophagus of each ferret to be dilated in the thoracic and cervical regions. of 4 ferrets that had lymphocytopenia, 2 had concurrent leukopenia. serum biochemical analysis revealed high activity of alanine transaminase (4 ferret ... | 1994 | 7961071 |
| review: absorption and metabolism of beta-carotene. | to better understand the potential function of beta-carotene (beta-c) in the prevention of cancer, greater knowledge of beta-c metabolism and a suitable animal model to mimic human beta-c metabolism are necessary. the small intestinal mucosa contains beta-c cleavage enzyme(s), thereby playing an important role in both the provitamin a activity and anti-cancer properties of beta-c. the ability of the ferret (mustela putorius furo) to absorb intact beta-c makes it an appropriate model for studying ... | 1994 | 7963135 |
| helicobacter pylori and the pathogenesis of duodenal ulcer. | in summary, it appears that the role of h. pylori in duodenal ulcerogenesis is not directly associated with acid hypersecretion. similarly, it seems unlikely that h. pylori-induced autoimmune injury is an important mechanism in duodenal ulcerogenesis. an essential question remaining is whether h. pylori infection of areas of gastric metaplasia in the duodenum is essential to ulcer pathogenesis. the low yield of h. pylori in duodenal biopsy studies argues against this mechanism. it is possible th ... | 1994 | 7964119 |
| transmissible mink encephalopathy species barrier effect between ferret and mink: prp gene and protein analysis. | experimental infection of transmissible mink encephalopathy (tme) in two closely related mustelids, black ferret (mustela putorius furo) and mink (mustela visa), revealed differences in their susceptibility to the tme agent. when challenged with the stetsonville tme agent, a longer incubation period was observed in ferrets (28 to 38 months) than mink (4 months). western blot analysis of ferret and mink prion proteins (prp) demonstrated no detectable differences between the proteins. northern blo ... | 1994 | 7964604 |
| control of steady-state intracellular ph in intact perfused ferret hearts. | to elucidate the role of bicarbonate-dependent mechanisms and na+/h+ exchange in maintenance of physiological intracellular ph (phi) under various steady-state conditions, phosphorus nmr spectra were taken in isovolumically-contracting, perfused ferret hearts. switching the perfusate from hco3-/co2 to hepes buffer significantly decreased phi from the control value of 7.14 +/- 0.01 (mean +/- s.e., n = 4) to 7.08 +/- 0.01 (p < 0.01). exposure to 4-acetamide-4'-isothiocyanatostilbene-2,2'-disulphon ... | 1994 | 7966350 |
| distinct laminar differences in the distribution of excitatory amino acid receptors in adult ferret primary visual cortex. | in order to explore the relative contributions of the different ionotropic excitatory amino acid receptor subtypes to signalling in primary visual cortex, we have mapped their distributions in area 17 of adult ferret cerebral cortex by quantitative in vitro autoradiography. d,l-alpha-amino-3-hydroxy-5-methoxy-4-isoxazole propionate (ampa) and kainate receptors, gating fast, na(+)-permeable channels, were localized with [3h]dizocilpine maleate ([3h]mk-801). all three radioligands bound to single ... | 1994 | 7969924 |
| ultra-slow voltage-dependent inactivation of the calcium current in guinea-pig and ferret ventricular myocytes. | l-type ca2+ current, ica, has been recorded in guinea-pig ventricular myocytes at 36 degrees c using the whole cell patch clamp technique. intracellular ca2+ was buffered with ethylenebis(oxonitrilo)tetraacetate (egta). an increase in the rate of stimulation from 0.5 to 3 hz resulted in an abrupt decrease in ica in the first beat at the high rate, followed by a progressive decrease (tau approx. 7 s) over the next 30 s. the changes were not the result of ca(2+)-dependent inactivation, because sim ... | 1994 | 7971160 |
| effect of cromakalim on skeletal muscle function and blood flow in the ferret ischemic hindlimb. | cromakalim, an agent that opens atp-modulated potassium channels, has recently been reported to reduce skeletal muscle contractile force during anoxia in vitro. to determine whether this activity occurs in vivo, cromakalim was tested for its ability to influence muscle function and blood flow in a model of skeletal muscle ischemia. in anesthetized ferrets the muscles of the hindlimb were stimulated electrically via the sciatic nerve and isometric force of contraction was measured. under normal p ... | 1994 | 7972324 |
| effect of sodium pyridinethione on the uptake and distribution of nickel in rats, ferrets and guinea-pigs. | oral administration of sodium pyridinethione together with ni2+ (using 63ni2+ as a tracer) to rats, ferrets and guinea-pigs produced highly increased tissue levels of the metal in several tissues in comparison with animals given the ni2+ alone. ni2+ forms a lipophilic complex with pyridinethione and it can be assumed that a facilitated passage of the ni2+ across the cellular membranes of various tissues is important for the observed effects. pigmented tissues (e.g. the eye melanin), the pancreat ... | 1994 | 7979962 |
| effects of bradykinin receptor agonists, and captopril and thiorphan in ferret isolated trachea: evidence for bradykinin generation in vitro. | bradykinin b2 receptor agonists, but not a b1 receptor agonist, were potent spasmogens of ferret isolated trachea. bradykinin-induced contractions were unaffected by several pharmacological agents, indicating a direct effect on airway smooth muscle b2 receptors. captopril plus thiorphan caused contractions in approximately 70% of preparations that were abolished by b2 receptor antagonists, but not by b1 receptor antagonist. thus, ferret tracheal tissues appear capable of releasing and degrading ... | 1994 | 7982459 |
| efficacy of 2'-deoxy-2'-fluororibosides against influenza a and b viruses in ferrets. | single-dose treatments (5 to 40 mg/kg of body weight given intraperitoneally) of ferrets with 2'-deoxy-2'-fluoroguanosine or its prodrug, 2,6-diamino-purine-2'-fluororiboside, 1 h after infection with influenza a virus significantly inhibited replication of virus in the upper respiratory tract, resulting in amelioration of fever and nasal inflammation. replication of virus in the lower respiratory tract was also reduced > 100-fold, but three doses were required to prevent replication in the lung ... | 1994 | 7986023 |
| functional mapping of horizontal connections in developing ferret visual cortex: experiments and modeling. | in cat striate cortex, patchy horizontal axonal projections link columns of similar orientation specificity. to assess the physiological correlates of such clustered projections, a new multisite stimulation technique was used to functionally map the pattern of horizontal synaptic inputs onto single layer 2/3 cells within tangential slices of developing ferret visual cortex. twenty-four separate sets of horizontal fibers were stimulated within a 1200 microns strip of cortex, while evoked synaptic ... | 1994 | 7996176 |
| model for the pharmacological basis of spontaneous synchronous activity in developing retinas. | spontaneous waves of bursts of action potentials propagate across the ganglion-cell surface of developing retinas. a recent biophysical model postulated that this propagation is mediated by an increase in extracellular k+, following its ejection from ganglion cells during action potentials. moreover, the model hypothesized that bursts might terminate due to the accumulation of intracellular ca2+ and the subsequent activation of a ca(2+)-dependent k+ conductance in the cells' dendrites. finally, ... | 1994 | 7996185 |
| subgrouping of respiratory syncytial virus strains from australia and papua new guinea by biological and antigenic characteristics. | strains of respiratory syncytial virus from 3 major areas of australia and papua new guinea (png) were analyzed for variations in their antigenic and biological properties and in the molecular weights of their major structural proteins. seventy-eight strains from infants and young children with lri were collected from 1981-1984. the rsv season in the australian cities lasted from april through september, with major peaks in july of each year, while the rsv season in tropical png was year-round, ... | 1994 | 8002781 |
| migration and development of the larvae of gnathostoma nipponicum in the rat, second intermediate or paratenic host, and the weasel, definitive host. | migration and development of the larvae of gnathostoma nipponicum in the rat as second intermediate or paratenic host and the weasel as definitive host were examined to clarify the pathogenesis in each animal. the advanced third-stage larvae (adl3) given orally to rats remained in the stomach for 12 h post-infection (pi). after that, larvae transiently invaded various organs and finally moved into the muscle within 48 h. the percentage of eosinophils in the blood taken from the jugular vein of r ... | 1994 | 8006379 |
| the inotropic mechanism of the phosphodiesterase inhibitor opc 3911 alone and in combination with rolipram, studied in papillary muscles of ferret and guinea-pig and isolated myocytes of guinea-pig ventricular muscle. | opc 3911 is a potent inhibitor and pde iii is a specific inhibitor in cardiac muscle. the effects of the drug alone and in combination with the non-inotropic pde iv inhibitor rolipram were analysed using hearts from guinea-pigs and ferrets. opc 3911 had an ec50 value of 0.1 microm. at 0.1 microm peak force was increased by 50.7 +/- 7.6% (n = 6, p < 0.001), time to peak tension (tpt) reduced by 18.7 +/- 5.6% (n = 6, p < 0.05). time to half relaxation (thr) was prolonged by 19 +/- 4.2% (n = 6, p < ... | 1994 | 8010139 |
| genome conservation in helicobacter mustelae as determined by pulsed-field gel electrophoresis. | genomic dna from 15 strains of helicobacter mustelae was subjected to pulsed-field gel electrophoresis (pfge) after digestion with paci and sfii. h. mustelae genome dna appeared very similar in all strains examined, whether isolated from ferrets or mink or from animals bred in either the usa or in the uk. the h. mustelae genome size was estimated to be 1.7 mb, similar in size to that of h. pylori. a minor difference in paci pfge pattern and genome size was observed between rifampicin-resistant a ... | 1994 | 8013879 |
| granulomatous enteritis caused by mycobacterium avium in a ferret. | granulomatous enteritis caused by mycobacterium avium was diagnosed in a pet ferret evaluated for anorexia, vomiting, and diarrhea. delayed gastric emptying was identified during barium sulfate contrast radiography. a constriction in the duodenum, which was an area of granulomatous inflammation on histologic examination, was surgically removed. after removal of this lesion, the ferret did well for 8 months, but clinical signs returned. at a second surgery, a nodule was removed from the pylorus. ... | 1994 | 8014091 |
| the metabolic consequences of an increase in the frequency of stimulation in isolated ferret hearts. | 1. the metabolic consequences of an increase in the frequency of stimulation were examined in isolated ferret hearts. intracellular ph (phi) and the intracellular concentrations of phosphocreatine ([pcr]i), inorganic phosphate ([pi]i) and atp were measured by 31p nuclear magnetic resonance (nmr) spectroscopy. 2. increasing the stimulus rate from 0.1-0.7 to 2 hz caused an increase in [pi]i and a decrease recovery of both [pcr]i and [pi]i during continued stimulation. there was no change in [atp]i ... | 1994 | 8014891 |
| further studies of the antiemetic activity of granisetron against whole body x-irradiation or cisplatin-induced emesis in the ferret. | in ferrets, the highly selective 5-ht3 receptor antagonist, granisetron, abolished or reduced emesis induced by cisplatin (10 mg/kg i.v.) or whole body x-irradiation (50 gy in 10.4 min) in a dose-dependent manner when administered by a variety of routes (intravenous, per os, subcutaneous, intramuscular). complete protection from vomiting and retching was achieved with 0.5 mg/kg i.v. or p.o. granisetron. granisetron (0.5 mg/kg i.v.) was also effective when given 6 h before cisplatin, completely p ... | 1994 | 8016189 |
| mechanism of the negative inotropic effect of propofol in isolated ferret ventricular myocardium. | the aim of this study was to investigate propofol's effect on myocardial contractility and relaxation and examine its underlying mechanism of action in isolated ferret ventricular myocardium. | 1994 | 8024141 |
| melanin and the regulation of mammalian photoreceptor topography. | melanin, or products directly associated with it, regulates the maturation of the neural retina because in hypopigmented mammals the central retina fails to develop fully. to determine whether this deficit is reflected in the distribution of photoreceptors, their topography has been studied in the retinae of normally reared pigmented and albino ferrets and animals reared under reduced light conditions. in both strains, the general distribution of rods and cones was similar to that in the cat, co ... | 1994 | 8025716 |
| a technique for catheterization of the urinary bladder in the ferret. | the technique of catheterization of the urinary bladder, an important clinical skill for the diagnosis of urinary tract disorders, has not been described for the ferret. the bladder was catheterized in 23 ferrets (10 intact females; 11 spayed females; and 2 intact males) using a 3 1/2 french, red rubber urethral catheter fitted with a steel wire stylet. ferrets were anaesthetized with isoflurane or ketamine (30 mg/kg im) and xylazine (3 mg/kg im). females were positioned in ventral recumbency wi ... | 1994 | 8035567 |
| enantioselective inhibition of apomorphine-induced emesis in the ferret by the neurokinin1 receptor antagonist cp-99,994. | these studies have examined the effects of the selective neurokinin1 (nk1) receptor antagonist cp-99,994 on the retching and vomiting response to apomorphine. cp-99,994 (1-3 mg/kg i.p.) attenuated retching and vomiting induced by apomorphine (0.25 mg/kg s.c.) with complete inhibition of retching and vomiting at the 3 mg/kg dose. in contrast cp-100,263 (3 mg/kg i.p.), the enantiomer of cp-99,994 with 1000-fold lower affinity for the nk1 receptor, was without effect. | 1994 | 8035913 |
| does early enucleation affect the decussation pattern of alpha cells in the ferret? | naturally occurring cell death has been hypothesized to sculpt various features of the organization of the mature visual pathways, including the recent proposal that the selective elimination of ganglion cells in the temporal retina shapes the formation of decussation patterns. through a class-specific interocular competition, ganglion cells in the two temporal hemiretinae are selectively lost to produce the decussation patterns characteristic of each individual cell class (leventhal et al., 198 ... | 1994 | 8038121 |
| relaxation in ferret ventricular myocytes: unusual interplay among calcium transport systems. | transport systems responsible for removing ca2+ from the myoplasm during relaxation in isolated ferret ventricular myocytes were studied using caffeine-induced contractures. internal calcium concentration ([ca2+]i) was measured with the fluorescent calcium indicator indo-1, and the results were compared with our recent detailed characterizations in rabbit and rat myocytes. relaxation and [ca2+]i decline during a twitch in ferret myocytes were fast and similar to that in rat myocytes (i.e. half-t ... | 1994 | 8046644 |
| genetic diversity of pneumocystis carinii derived from infected rats, mice, ferrets, and cell cultures. | the degree of strain and/or species diversity among pneumocystis carinii isolates is unknown. as a first approach to the study of p. carinii genetic relatedness, we compared the pulsed field gel electrophoretic karyotypes of p. carinii derived from lung homogenates of three immunosuppressed host animals: rats transtracheally inoculated with p. carinii-infected mouse lung; and ferrets which developed reactivated latent p. carinii pneumonia. rat p. carinii propagated on hel299 cells was also exami ... | 1994 | 8049685 |
| intracellular campylobacter-like organism from ferrets and hamsters with proliferative bowel disease is a desulfovibrio sp. | proliferative bowel disease is an intestinal disorder of a variety of domestic animals associated with the presence of an intracellular campylobacter-like organism (iclo). we have identified the iclo obtained from a ferret with proliferative colitis by 16s rrna sequence analysis. in this ferret, proliferative bowel tissue containing the iclo had translocated to the mesenteric lymph nodes, omentum, and liver. the 16s rrna genes of the iclo were amplified from an infected fragment of extraintestin ... | 1994 | 8051249 |
| age-related effects of triphenyl phosphite-induced delayed neuropathy on central visual pathways in the european ferret (mustela putorius furo). | the objective of this study was to investigate the relationship between the maturation of visual system neurons and the onset of their susceptibility to triphenyl phosphite (tpp)-induced delayed neurotoxicity in the european ferret. we administered single subcutaneous doses of tpp (1184 mg/kg body wt) to 1- to 10-week-old ferret kits to assess the effects on connections and neurons of the developing lateral geniculate thalamic nucleus (lgn) and primary visual cortex. brains were processed with a ... | 1994 | 8056204 |
| influence of callosal activity on units in the auditory cortex of ferret (mustela putorius). | 1. callosal neurons in primary auditory cortex project heavily to regions of the contralateral homotopic field that are excited by stimulation of one or both ears and minimally to regions that are excited by stimulation of one ear and inhibited by stimulation of the other ear. the intent of this study is to begin assessing the function of this extensive intercortical projection in determining the response properties of single units in the recipient auditory cortex. 2. callosal neurons in the rig ... | 1994 | 8064345 |
| effects of a novel nonpeptide bradykinin b2 receptor antagonist on intestinal and airway smooth muscle: further evidence for the tracheal b3 receptor. | 1. we examined the effects of phosphonium, [[4-[[2- [[bis(cyclohexylamino)methylene]amino]-3-(2-naphthalenyl) 1-oxopropyl]amino]-phenyl]-tributyl, chloride, monohydrochloride (win 64338), a novel, nonpeptide bradykinin b2 receptor antagonist, on bradykinin-induced contractions of guinea-pig isolated ileum, and guinea-pig and ferret trachea. 2. win 64338 potently and competitively antagonized ileal contractions, in response to bradykinin, exhibiting a pa2 value of 7.97 +/- 0.10. the compound was ... | 1994 | 8075864 |
| efficacy of adenovirus-vectored respiratory syncytial virus vaccines in a new ferret model. | in the absence of an adequate small animal model for testing the efficacy of adenovirus-vectored respiratory syncytial virus (rsv) vaccines, a ferret model was established for this purpose. recombinant adenovirus types 4, 5 and 7 expressing the rsv fusion glycoprotein (f), the attachment glycoprotein (g) or both f and g were constructed previously. these recombinants contain a deletion of a large portion of the e3 region of the respective adenovirus vector. in addition, an ad7(e3+)f recombinant ... | 1994 | 8085377 |
| cortical specification: microcircuits, perceptual identity, and an overall perspective. | the microcircuitry within a cortical area and its perceptual identity are both specified relatively late in development. we have asked whether and how the pattern of input activity during development influences these features of cortex. routing visual projections to the auditory pathway in ferrets leads to visual activation of the developing auditory cortex, causing auditory cortex to receive patterns of input activity very different from normal. visual inputs respecify, in instructive fashion, ... | 1993 | 8087534 |
| ca2+ handling and myofibrillar ca2+ sensitivity in ferret cardiac myocytes with pressure-overload hypertrophy. | experiments were performed in aequorin-loaded left ventricular myocytes isolated from hypertrophied hearts and age-matched controls. five to six months after postvalvular aortic banding, left ventricular hypertrophy was present, as indicated by a 97% (p < 0.001) increase in the left ventricular weight-to-body weight ratio and a 24% (p < 0.001) increase in cell width. in comparison with controls, the hypertrophied myocytes demonstrated that 1) contraction duration was prolonged by 37% (p < 0.001) ... | 1994 | 8092296 |
| ca-dependent facilitation of cardiac ca current is due to ca-calmodulin-dependent protein kinase. | repetitive membrane potential (em) depolarization from -90 to 0 mv in rabbit and ferret ventricular myocytes induces a facilitation or "staircase" of ca current (ica), which is ca (not em) dependent and takes several seconds to accumulate and dissipate. that is, ica at the tenth pulse at 1-2 hz exceeds that at the first pulse (i10 > i1). the ica staircase was completely abolished by dialysis with either of two inhibitory peptides of ca-calmodulin-dependent protein kinase (camkii) camkii(290-309) ... | 1994 | 8092302 |
| immunolocalization of scrapie amyloid in non-congophilic, non-birefringent deposits in golden syrian hamsters with experimental transmissible mink encephalopathy. | transmissible mink encephalopathy (tme), a naturally occurring subacute spongiform encephalopathy in commercially ranch-reared mink (mustela vision), is characterized neuropathologically by spongiform changes in the neuropil, intracytoplasmic neuronal vacuolation and astrocytic hypertrophy and hyperplasia. amyloid deposits have not been observed in brain tissue sections from animals with natural and experimental tme using conventional histochemical stains such as congo red. to determine if amylo ... | 1993 | 8103204 |
| the effects of orally administered y-25130, a selective serotonin3-receptor antagonist, on chemotherapeutic agent-induced emesis. | the antiemetic effects of orally administered y-25130, a potent and selective 5-ht3-receptor antagonist, were compared with those of ondansetron, granisetron, metoclopramide and domperidone. y-25130 (0.1-1.0 mg/kg) dose-dependently prolonged the latency to the first vomiting and decreased the number of vomitings induced by cisplatin in dogs. the antiemetic effect of y-25130 against cisplatin-induced vomiting was more potent than that of metoclopramide and ondansetron, but it showed little differ ... | 1993 | 8107329 |
| ferrets. | this article addresses the common ferret diseases encountered by the veterinarian. an introductory section familiarizes the veterinarian with management and preventive health aspects of the pet ferret. associated clinical techniques are described. diseases most frequently seen in pet ferrets are discussed. | 1994 | 8109068 |
| tension and intracellular calcium transients of activated ferret ventricular muscle in response to step length changes. | to elucidate the effects of mechanical constraints on the (ca2+) affinity of cardiac troponin c, we studied the relationships among the myoplasmic ca2+ concentration ([ca2+]i), tension and length in steadily activated intact cardiac muscle. the ca2+ sensitive photoprotein, aequorin, was micro-injected into cells of ferret right ventricular papillary muscles to monitor the [ca2+]i. the muscle was then steadily activated with ouabain (10(-4) m)(ouabain contracture) or high frequency stimuli in the ... | 1993 | 8109375 |
| different sensitivities of the na+/k(+)-atpase isoforms to oxidants. | inhibition of na+/k(+)-atpase by partially reduced oxygen metabolites has been suggested to be involved in ischemia-reperfusion injury to heart and other organs. since various isoforms of the enzyme have different sensitivities to ouabain and several other inhibitors, we studied the effects of h2o2 and the hydroxyl radical on enzyme activity and phosphoenzyme formation in na+/k(+)-atpase preparations with known alpha-subunit isoform composition in order to assess the oxidant sensitivities of the ... | 1994 | 8110803 |
| estimates of beat to beat handling of activator calcium using measurements of [ca2+]i in aequorin loaded ferret cardiac muscle. | the aims were (1) to measure simultaneously and on a beat to beat basis intracellular calcium concentration ([ca2+]i) transients and force transients in isolated ferret cardiac trabeculae; (2) to obtain and compare independent estimates of the recirculating fraction of ca2+ using the [ca2+]i data and the force data (recirculating fraction is the fraction of activator ca2+ taken up by the sarcoplasmic reticulum in each beat and, in the steady state twitch, the fraction of activator ca2+ released ... | 1994 | 8111791 |
| histopathologic features and post-surgical sequelae of 57 cutaneous neoplasms in ferrets (mustela putorius furo l.). | the study of the signalment, histomorphologic features, and post-surgical clinical progress of 57 cutaneous neoplasms in 55 domestic ferrets (mustela putorius furo l.) was based on diagnostic pathologic accessions (1987-1992) from 142 ferrets. mean age of the group was 4.3 years; 31/54 (57%) were female and 23/54 (43%) were male. thirty-three (58%) of the cutaneous neoplasms were basal cell tumors. the mean age of ferrets with basal cell tumor was 5.2 years, and 23/33 (70%) were female. histolog ... | 1993 | 8116142 |
| codominant mixtures of viruses in reference strains of influenza virus due to host cell variation. | influenza viruses grown in chicken eggs may comprise mixtures of variants, creating problems in establishing international reference strains and in preparing high growth reassortants. we therefore analyzed representative reference strains of h3n2 viruses from 1987 to 1989 by direct sequencing of ha1. three of seven reference strains had different nucleotides at the same position in nucleotide gels, indicating the presence of codominant mixtures. these nucleotide duplications occurred at residues ... | 1994 | 8116258 |
| the tachykinin nk1 receptor antagonist cp-99,994 attenuates cisplatin induced emesis in the ferret. | the tachykinin nk1 receptor antagonist cp-99,994 ((+)-(2s,3s)-3-(2-methoxybenzylamino)-2-phenylpiperidine) (0.3-3 mg/kg i.v.), but not its inactive enantiomer cp-100,263, attenuated the retching and vomiting induced by cisplatin (10 mg/kg i.v.) in the ferret. cp-99,994, 3 mg/kg i.v., prevented vomiting in all ferrets tested. since substance p is the preferred ligand at the nk1 receptor subtype these data support a role for the release of this peptide during the emetic response induced by cytotox ... | 1993 | 8119305 |
| modulation of basal l-type ca2+ current by adenosine in ferret isolated right ventricular myocytes. | 1. the whole-cell configuration of the gigaohm seal voltage clamp and an internal perfusion technique were used to study the effects of adenosine on the basal l-type ca2+ current (ica) in enzymatically isolated right ventricular myocytes of ferrets. basal l-type ica was isolated by using a na(+)- and k(+)-free saline (replacement by n-methyl-d-glucamine+, cs+ and tea+, respectively). all experiments were conducted at room temperature (22-24 degrees c). 2. basal ica was markedly reduced during ex ... | 1993 | 8120807 |
| modulation of l-type ca2+ current by extracellular atp in ferret isolated right ventricular myocytes. | 1. the effects of extracellular adenosine triphosphate (atp) on the basal l-type ca2+ current (ica) were investigated in ferret isolated right ventricular myocytes using the gigaohm seal voltage clamp in the whole-cell and cell-attached configurations. 2. micromolar levels of extracellular atp reversibly inhibited ica in a concentration-dependent manner, without any significant changes in the voltage dependence of either the peak ica i-v relationship or steady-state activation curve. 3. in contr ... | 1993 | 8120808 |
| pneumocystis carinii pneumonia in thoroughbred foals: identification of a genetically distinct organism by dna amplification. | genetically distinct forms of pneumocystis carinii infect several mammalian hosts. we report the amplification of p. carinii dna from samples of two infected thoroughbred foal lungs by using primers designed from the sequence of a p. carinii mitochondrial rrna gene; these primers also prime the amplification of p. carinii dna from other hosts. the nucleotide sequence of part of the mitochondrial rrna gene amplified from p. carinii infecting one of the foals was determined and found to be distinc ... | 1994 | 8126183 |
| regulation of neurotrophin receptors during the maturation of the mammalian visual system. | cell division, cell death, and remodeling of connections are major features of the construction of the mammalian cns. we have begun to address the role of neurotrophins in these events through characterization of the expression of their receptors in the developing ferret visual system. by use of chemical cross-linking of iodinated neurotrophins, proteins corresponding to trkb, trkc, and p75 were identified as receptors for brain-derived neurotrophic factor (bdnf) and neurotrophin-3 (nt-3) throug ... | 1994 | 8126572 |
| evaluation of the bioavailability of natural and synthetic forms of beta-carotenes in a ferret model. | | 1993 | 8129298 |
| distribution of beta-carotene and vitamin a in lipoprotein fractions of ferret serum. effect of beta-carotene supplementation. | | 1993 | 8129299 |