| development of tools for quantitative intracellular metabolomics of aspergillus niger chemostat cultures. | in view of the high citric acid production capacity of aspergillus niger, it should be well suited as a cell factory for the production of other relevant acids as succinic, fumaric, itaconic and malic. quantitative metabolomics is an important omics tool in a synthetic biology approach to develop a. niger for the production of these acids. such studies require well defined and tightly controlled cultivation conditions and proper rapid sampling, sample processing and analysis methods. in this stu ... | 2017 | 26366135 |
| 2,4-di-tert-butyl phenol as the antifungal, antioxidant bioactive purified from a newly isolated lactococcus sp. | the volatile organic compound 2,4-di-tert-butyl phenol (2,4 dtbp) was purified from the cell free supernatant of a newly isolated lactococcus sp. by solvent extraction and chromatographic techniques. molecular characterization of the compound by esi-ms, (1)h nmr and ftir analysis revealed the structure, c14h22o. fungicidal activity was demonstrated against aspergillus niger, fusarium oxysporum and penicillium chrysogenum by disc diffusion assay. among the cell lines tested for cytotoxicity of th ... | 2015 | 26164257 |
| effects of three volatile oxylipins on colony development in two species of fungi and on drosophila larval metamorphosis. | the aim of this study is to investigate the effects of three volatile oxylipins on colony development in two fungi and on drosophila larval metamorphosis. using an airborne exposure technique, three common and volatile oxylipins (1-octen-3-ol, (e)-2-hexenal, and 1-hexanol) were compared for their effects on spore germination and colony growth in aspergillus niger and penicillium chrysogenum, as well as for their effects on the morphogenesis of larvae of drosophila melanogaster. conidia of both a ... | 2015 | 26126831 |
| environmental mold and mycotoxin exposures elicit specific cytokine and chemokine responses. | molds can cause respiratory symptoms and asthma. we sought to use isolated peripheral blood mononuclear cells (pbmcs) to understand changes in cytokine and chemokine levels in response to mold and mycotoxin exposures and to link these levels with respiratory symptoms in humans. we did this by utilizing an ex vivo assay approach to differentiate mold-exposed patients and unexposed controls. while circulating plasma chemokine and cytokine levels from these two groups might be similar, we hypothesi ... | 2015 | 26010737 |
| synthesis, spectral, thermal, fluorescence, antimicrobial, anthelmintic and dna cleavage studies of mononuclear metal chelates of bi-dentate 2h-chromene-2-one schiff base. | the co(ii), ni(ii) and cu(ii) complexes have been synthesized with schiff base (hl), derived from 8-formyl-7-hydroxy-4-methylcoumarin with benzylamine. the schiff base and its metal complexes were structurally characterized based on ir, (1)h nmr, (13)c nmr, uv-visible, esr, magnetic, thermal, fluorescence, mass and esi-ms studies. the complexes are completely soluble in dmf and dmso. the molar conductance values indicate that, all synthesized metal complexes are non-electrolytic in nature. eleme ... | 2015 | 26002539 |
| protein kinase a signaling and calcium ions are major players in paf mediated toxicity against aspergillus niger. | the penicillium chrysogenum antifungal protein paf is toxic against potentially pathogenic ascomycetes. we used the highly sensitive aequorin-expressing model aspergillus niger to identify a defined change in cytoplasmic free ca(2+) dynamics in response to paf. this ca(2+) signature depended on an intact positively charged lysine-rich paf motif. by combining ca(2+) measurements in a. niger mutants with deregulated camp/protein kinase a (pka) signaling, we proved the interconnection of ca(2+) per ... | 2015 | 25882631 |
| untargeted metabolic profiling of vitis vinifera during fungal degradation. | this paper illustrates the application of an untargeted metabolic profiling analysis of winery-derived biomass degraded using four filamentous fungi (trichoderma harzianum, aspergillus niger, penicillium chrysogenum and p. citrinum) and a yeast (saccharomyces cerevisiae). analysis of the metabolome resulted in the identification of 233 significant peak features [p < 0.05; fold change (fc) > 2 and signal-to-noise ratio >50] using gas chromatography-mass spectrometry followed by statistical chemom ... | 2015 | 25868913 |
| biotic elicitor enhanced production of psoralen in suspension cultures of psoralea corylifolia l. | cell cultures of psoralea corylifolia l. were established from the leaf disk derived callus. the effect of different biotic elicitors prepared from the fungal extract (aspergillus niger and penicillium notatum), yeast extract and chitosan with different concentrations was studied. the increased synthesis of psoralen in 16-day old cell cultures under 16 h of light and 8 h of dark period was studied. elicitation of psoralen in a. niger elicitor treated cells was found 9-fold higher over control ce ... | 2014 | 25313287 |
| synthesis of mannich bases: 2-(3-phenylaminopropionyloxy)-benzoic acid and 3-phenylamino-1-(2,4,6-trimethoxy-phenyl)-propan-1-one, their toxicity, ionization constant, antimicrobial and antioxidant activities. | mannich bases 2-(3-phenylaminopropionyloxy)-benzoic acid (a) and 3-phenylamino-1-(2,4,6-trimethoxy-phenyl)-propan-1-one (b) were synthesized. structures were confirmed by ultraviolet/visible and infra-red spectroscopies. the ionization constant (pka) values at 8.3 and 8.0 reported for compounds a and b, respectively, indicated that protonation might occur at physiological ph. the lc50 values of 145,595 μg/ml (a) and 82,526 μg/ml (b) obtained from brine shrimp lethality testing showed that both c ... | 2014 | 25038706 |
| the use of real-time pcr to study penicillium chrysogenum growth kinetics on solid food at different water activities. | fungal growth on solid foods can make them unfit for human consumption, but certain specialty foods require fungi to produce their characteristic properties. in either case, a reliable way of measuring biomass is needed to study how various factors (e.g. water activity) affect fungal growth rates on these substrates. biochemical markers such as chitin, glucosamine or ergosterol have been used to estimate fungal growth, but they cannot distinguish between individual species in mixed culture. in t ... | 2014 | 25036772 |
| lag time for germination of penicillium chrysogenum conidia is induced by temperature shifts. | in the environment, fungal conidia are subject to transient conditions. in particular, temperature is varying according to day/night periods. all predictive models for germination assume that fungal spores can adapt instantaneously to changes of temperature. the only study that supports this assumption (gougouli and koutsoumanis, 2012, modelling germination of fungal spores at constant and fluctuating temperature conditions. international journal of food microbiology, 152: 153-161) was carried o ... | 2014 | 24929731 |
| antifungal effect of antimicrobial peptides (amps lr14) derived from lactobacillus plantarum strain lr/14 and their applications in prevention of grain spoilage. | the concern for food safety has led to an increased interest in the development of novel antimicrobials. keeping this aim in mind, we have investigated the antifungal effect of antimicrobial peptides (amps lr14) produced by lactobacillus plantarum strain lr/14 against four spoilage fungi, namely, aspergillus niger, rhizopus stolonifer, mucor racemosus and penicillium chrysogenum. interestingly, all the four fungi were inhibited, suggesting that amps lr14 exhibited anti-fungal property. the pepti ... | 2014 | 24929709 |
| effect of low shear modeled microgravity on phenotypic and central chitin metabolism in the filamentous fungi aspergillus niger and penicillium chrysogenum. | phenotypic and genotypic changes in aspergillus niger and penicillium chrysogenum, spore forming filamentous fungi, with respect to central chitin metabolism were studied under low shear modeled microgravity, normal gravity and static conditions. low shear modeled microgravity (lsmmg) response showed a similar spore germination rate with normal gravity and static conditions. interestingly, high ratio of multiple germ tube formation of a. niger in lsmmg condition was observed. confocal laser scan ... | 2014 | 24803238 |
| control of spoilage fungi by protective lactic acid bacteria displaying probiotic properties. | thirty-six lactic acid bacteria belong to lactococcus, lactobacillus, enterococcus, and pediococcus were isolated, and the spectrum of antifungal activity was verified against fusarium oxysporum (kacc 42109), aspergillus niger (kacc 42589), fusarium moniliforme (kacc 08141), penicillium chrysogenum (nii 08137), and the yeast candida albicans (mtcc 3017). three isolates, identified as pediococcus pentosaceus (tg2), lactobacillus casei (dy2), and lactococcus (bsn) were selected further, and their ... | 2014 | 24532445 |
| gemini alkyldeoxy-d-glucitolammonium salts as modern surfactants and microbiocides: synthesis, antimicrobial and surface activity, biodegradation. | dimeric quaternary alkylammonium salts possess a favourable surface and antimicrobial activity. in this paper we describe synthesis, spectroscopic analysis, surface and antimicrobial activity as well as biodegradability of polymethylene-α,ω-bis(n,n-dialkyl-n-deoxy-d-glucitolammonium iodides), a new group of dimeric quaternary ammonium salts. this new group of gemini surfactants can be produced from chemicals which come from renewable sources. the structure of products has been determined by the ... | 2014 | 24416314 |
| fungal degradation of an acetolactate synthase (als) inhibitor pyrazosulfuron-ethyl in soil. | owing to reported phytotoxicity of some sulfonylurea class of herbicides in number of sensitive crops and higher persistence in soil, present study was conducted to isolate and identify pyrazosulfuron-ethyl degrading fungi from soil of rice field. penicillium chrysogenum and aspergillus niger, were isolated and identified from rhizospere soil of rice field, as potent pyrazosulfuron-ethyl degrading fungi. degradation of pyrazosulfuron-ethyl by p. chrysogenum and a. niger, yielded transformation p ... | 2013 | 23993642 |
| a comparative study of nitrilases identified by genome mining. | escherichia coli strains expressing different nitrilases transformed nitriles or kcn. six nitrilases (from aspergillus niger (2), a. oryzae, neurospora crassa, arthroderma benhamiae, and nectria haematococca) were arylacetonitrilases, two enzymes (from a. niger and penicillium chrysogenum) were cyanide hydratases and the others (from p. chrysogenum, p. marneffei, gibberella moniliformis, meyerozyma guilliermondi, rhodococcus rhodochrous, and r. ruber) preferred (hetero)aromatic nitriles as subst ... | 2013 | 23475593 |
| influence of fungal elicitors on biosynthesis of natamycin by streptomyces natalensis hw-2. | to investigate the effect of fungal elicitors on biosynthesis of natamycin in the cultures of streptomyces natalensis hw-2, the biomass and filtrate of the broth from aspergillus niger as 3.6472, penicillium chrysogenum as 3.5163, a. oryzae as 3.2068, and saccharomyces cerevisiae as 2.2081 were used to induce natamycin production in s. natalensis hw-2. the results showed that the biomass of p. chrysogenum as 3.5163 could enhance the yield of natamycin from 0.639 to 0.875 g l(-1). the elicitor fr ... | 2013 | 23463250 |
| safety evaluation of glucose oxidase from penicillium chrysogenum. | glucose oxidase (β-d-glucose:oxygen 1-oxidoreductase; ec 1.1.2.3.4) is used in the food and beverage industry as a preservative and stabilizer and is commonly derived from the fungus aspergillus niger. although the safety of glucose oxidase preparations from a. niger is well-established, the use of preparations derived from other fungal species is of interest; however, an assessment of their safety is warranted. here, we report on the safety of a glucose oxidase preparation derived from the fung ... | 2013 | 23454104 |
| design, synthesis, spectral and biological evaluation of novel 1-allyl substituted 2,6-diphenylpiperidin-4-ones and its derivatives of oximes/oxime ethers. | a series of 1-allyl-2,6-diphenylpiperidin-4-one oximes 17-24 and 1-allyl-2,6-diphenylpiperidin-4-one o-benzyloximes 25-32 were synthesized from respective 1-allyl-2,6-diphenylpiperidin-4-ones 9-16. the structure and the conformations have been investigated for all the synthesized compounds 9-32 by analytical and spectral techniques (ir, mass, 1h, 13c, 2d nmr and x-ray diffraction). based on the proton nmr analysis, all the synthesized compounds 9-32, exposed chair conformation except compounds 2 ... | 2012 | 23031593 |
| phytochemical screening and antimicrobial activity of coccinia cordifolia l. plant. | the medicinal plant, coccinia cordifolia l. was analyzed for its chemical composition. the antimicrobial activities of the methanol, water, ethanol and ethyl acetate extracts of coccinia cordifolia l. plant were evaluated against some gram positive bacteria (sarcina lutea, bacillus subtilis and staphylococcus aureus), gram negative bacteria (salmonella typhi, shigella dysenteriae and escherichia coli) and fungi (candida albicans, aspergillus niger and penicillium notatum). chemical analysis show ... | 2012 | 23009991 |
| influence of substituent effects on spectroscopic properties and antimicrobial activity of 5-(4'-substituted phenylazo)-2-thioxothiazolidinone derivatives. | 5-(4'-substituted phenylazo)-2-thioxothiazolidinone derivatives (hl(n)) have been synthesized and characterized by elemental analysis, spectra (ir, electronic and (1)h nmr). the ir spectral data indicate that the compounds can exist in two resonance structures. the synthesized ligands were screened for their antimicrobial activity against four bacterial species, two gram positive bacteria (bacillus cereus and staphylococcus aureus) and two gram negative bacteria (escherichia coli and klebsiella ... | 2013 | 22968655 |
| chemical composition and antimicrobial activity of the leaf essential oil of skimmia laureola growing wild in jammu and kashmir, india. | the analysis of skimmia laureola hydrodistillate by gas chromatography coupled with mass spectrometry revealed the presence of 20 constituents, representing 94.6% of the total oil. the major constituents of oil were linalyl acetate (33.0%), linalool (25.0%), limonene (8.1%), α-terpineol (5.9%) and geranyl acetate (5.9%). the monoterpene (93.4%) rich essential oil was evaluated for its antibacterial and antifungal activities against seven microorganisms by agar diffusion and microdilution methods ... | 2013 | 22780302 |
| diversity of microfungi in sandy beach soil of teluk aling, pulau pinang. | a total of 82 isolates of microfungi were isolated from 6 sandy soil samples collected from teluk aling beach, pulau pinang. the soil microfungi were isolated by using direct isolation, debris isolation and soil dilution techniques. based on morphological characteristics, seven genera of microfungi were identified namely, fusarium (42%), aspergillus (24%), trichoderma (13%), curvularia (9%), colletotrichum (6%), helminthosporium (4%) and penicillium (2%). the most common species isolated was fus ... | 2011 | 24575210 |
| isolation and characterization of formaldehyde-degrading fungi and its formaldehyde metabolism. | formaldehyde is classified as a human carcinogen that may cause nasopharyngeal cancer and probably leukemia. the effects of environmental and nutritional factors on fungal growth and the biodegradation of formaldehyde were investigated. fungal strains sgfa1 and sgfa3 isolated from untreated sewage sediment samples collected from heavily formaldehyde-contaminated areas were identified using morphological characteristics and molecular techniques and named as aspergillus nomius sgfa1 and penicilliu ... | 2014 | 24464080 |
| fermentative recovery of lipids and proteins from freshwater fish head waste with reference to antimicrobial and antioxidant properties of protein hydrolysate. | effectiveness of fermentation using lactic acid bacteria (lab) for recovering lipids and proteins simultaneously from freshwater fish head (fwh) was evaluated. three different proteolytic lab (pediococcus acidilactici ncim5368, enterococcus faecium ncim5335 and pediococcus acidilactici fd3) isolated from fish processing wastes were employed in the fermentation process. the fermentation conditions involved 10 % (w/w) glucose, 2 % (w/w) nacl and 10 % (v/w) lab cultures at 37 °c. the process result ... | 2014 | 25190843 |
| identification of a novel penicillium chrysogenum rhamnogalacturonan rhamnohydrolase and the first report of a rhamnogalacturonan rhamnohydrolase gene. | rhamnogalacturonan (rg) i is one of the main components of pectins in the plant cell wall. we recently reported two rg i-degrading enzymes, endo-rg and exo-rg lyases, secreted by penicillium chrysogenum 31b. here, our aims were to purify a rg rhamnohydrolase (pcrgrh78a) from the culture filtrate of this strain and to characterize this enzyme. on the basis of the internal amino acid sequences, the encoding gene, pcrgrh78a, was cloned and overexpressed in aspergillus oryzae. the deduced amino acid ... | 2017 | 28110667 |
| molecular characterization of a penicillium chrysogenum exo-rhamnogalacturonan lyase that is structurally distinct from other polysaccharide lyase family proteins. | we previously described an endo-acting rhamnogalacturonan (rg) lyase, termed pcrgl4a, of penicillium chrysogenum 31b. here, we describe a second rg lyase, called pcrglx. we determined the cdna sequence of the pcrglx gene, which encodes pcrglx. based on analyses using a blast search and a conserved domain search, pcrglx was found to be structurally distinct from known rg lyases and might belong to a new polysaccharide lyase family together with uncharacterized fungal proteins of nectria haematoco ... | 2015 | 25921806 |
| synthesis, characterization and antifungal activity of chemically and fungal-produced silver nanoparticles against trichophyton rubrum. | to characterize and explore the potential in extracellular biosynthesis of silver nanoparticles (agnps) by penicillium chrysogenum and aspergillus oryzae and to investigate the antifungal effect of chemically vs biologically synthesized agnps comparing with conventional antifungal drugs against trichophyton rubrum. | 2014 | 25234047 |
| molecular identification of isolated fungi from stored apples in riyadh, saudi arabia. | fungi causes most plant disease. when fruits are stored at suboptimal conditions, fungi grows, and some produce mycotoxin which can be dangerous for human consumption. studies have shown that the penicillium and monilinia species commonly cause spoilage of fruits, especially apples. several other genera and species were reported to grow to spoil fruits. this study was conducted to isolate and identify fruit spoilage by fungi on apples collected in riyadh, saudi arabia and conduct a molecular ide ... | 2013 | 24235866 |
| fungi in ontario maple syrup & some factors that determine the presence of mold damage. | maple syrup is a high value artisanal product produced mainly in canada and a number of states primarily in the northeast usa. mold growth (wallemia sebi) on commercial product was first reported in syrup in 1908. since then, few data have been published. we conducted a systematic examination for fungi in maple syrup from 68 producers from all of the syrup-producing areas of ontario, canada. the mean ph of the samples was ph 6.82, sugar content averaged 68.0±0.89 °brix and aw averaged 0.841±0.01 ... | 2015 | 26001061 |
| interactions between naturally occurring antifungal agents. | pairwise interactions between four antifungal compounds were studied. the β-1,3-glucan synthase inhibitor echinocandin b (ecb) showed synergistic effect with the cell wall hydrolase chib chitinase and enga β-1,3-glucanase on saccharomyces cerevisiae, candida albicans, aspergillus rugulosus and a. fumigatus. the antifungal protein of penicillium chrysogenum (paf) did not influence the antifungal activity of chib or enga, but showed antagonistic effect with ecb on a. nidulans, a. rugulosus and a. ... | 2013 | 24275596 |
| molecular characterization and analysis of antimicrobial activity of endophytic fungi from medicinal plants in saudi arabia. | endophytic fungi, which have been reported in numerous plant species, are important components of the forest community and contribute significantly to the diversity of natural ecosystems. | 2016 | 27099679 |
| diversity and bioprospection of fungal community present in oligotrophic soil of continental antarctica. | we surveyed the diversity and capability of producing bioactive compounds from a cultivable fungal community isolated from oligotrophic soil of continental antarctica. a total of 115 fungal isolates were obtained and identified in 11 taxa of aspergillus, debaryomyces, cladosporium, pseudogymnoascus, penicillium and hypocreales. the fungal community showed low diversity and richness, and high dominance indices. the extracts of aspergillus sydowii, penicillium allii-sativi, penicillium brevicompac ... | 2015 | 25809294 |
| cytotoxic and antibiotic cyclic pentapeptide from an endophytic aspergillus tamarii of ficus carica. | a new cyclic pentapeptide, disulfide cyclo-(leu-val-ile-cys-cys) (1), named malformin e, together with 13 known cyclic dipeptides, was isolated from the culture broth of endophytic fungus fr02 from the roots of ficus carica. the strain fr02 was identified as aspergillus tamarii on the basis of morphological characteristics and molecular analyses of internal transcribed spacer (its). their structures were determined by the combination of 1d and 2d nmr spectroscopy, hrms (esi), uv, and marfey's an ... | 2016 | 27147299 |
| the capability of fungi isolated from moldy dwellings to produce toxins. | the main objective was analysis and assessment of toxinogenic capabilities of fungi isolated from moldy surfaces in residential rooms in an urban agglomeration situated far from flooded areas in moderate climate zone. | 2016 | 27518891 |
| effect of two aerosolization methods on the release of fungal propagules from a contaminated agar surface. | the effect of perpendicular and swirling aerozolization methods on the release of fungal fragments and spores from agar surface was studied. three fungal species (aspergillus versicolor, cladosporium cladosporioides, penicillium chrysogenum) were selected for the tests as they commonly occur indoors, create different hyphae structure when they grow on surfaces, and have different spore shapes, aerodynamic sizes, and formation mechanisms. as the tested surface, petri dishes filled with malt extra ... | 2012 | 22742802 |
| microbiological evaluation of ten french archives and link to occupational symptoms. | fungi that damage documents in archives may harm workers' health, depending on which mold species are inhaled, the concentrations of fungal species inhaled, and individual factors. our aim was to identify and quantify fungi in archives and to investigate possible links with the symptoms experienced by workers. ten french archives were sampled using an air impactor and electrostatic dust collectors. allergies and general symptoms felt by 144 workers were reported using a self-report questionnaire ... | 2012 | 22429323 |
| humidifier lung and humidifier fever. | the aim of our study was to evaluate clinical diagnoses in symptomatic persons exposed to aerosols from humidifiers or air conditioners. in addition, we tried to identify the causative antigens. results of clinical investigations, including inhalation challenge tests, demonstrated a typical hypersensitivity pneumonitis (humidifier lung) in 9 persons and isolated systemic symptoms without significant changes in lung function and chest x-rays (humidifier fever) in 3 persons. microbiological studie ... | 1988 | 27519372 |
| quantification and identification of microorganisms found on shell and kernel of fresh edible chestnuts in michigan. | chestnut is a relatively new cultivated crop for michigan, and postharvest loss due to decay has been problematic as production has increased each year. in 2007, more than 25% of chestnuts were lost to postharvest decay, equivalent to approximately 5300 kg of fresh product. to determine the organisms responsible for decay, a microbiological survey was performed in 2006 and 2007 to identify microorganisms involved in postharvest shell (external surface) mold and internal kernel (edible portion) d ... | 2016 | 26869338 |
| synthesis, characteristics and antimicrobial activity of zno nanoparticles. | the utilization of various plant resources for the bio synthesis of metallic nano particles is called green technology and it does not utilize any harmful protocols. present study focuses on the green synthesis of zno nano particles by zinc carbonate and utilizing the bio-components of powder extract of dry ginger rhizome (zingiber officinale). the zno nano crystallites of average size range of 23-26 nm have been synthesized by rapid, simple and eco friendly method. zinc oxide nano particles wer ... | 2015 | 25748589 |
| mycotoxins from mould infested building materials. | only limited documentation of non-allergenic, especially toxic reactions after inhalation of microfungal spores in water damaged buildings exists. recently attention has been drawn to the mycotoxins as causal compounds, as some the dominating genera found in buildings are well known mycotoxin producers.penicillium chrysogenum and a. ustus do not seem to produce any known mycotoxins when growing on building materials, whereasp. brevicompactum produces mycophenolic acid, somep. polonicum produces ... | 2000 | 23605430 |
| stereoselective bioreduction of α-azido ketones by whole cells of marine-derived fungi. | seven strains of marine-derived fungi (aspergillus sclerotiorum cbmai 849, cladosporium cladosporioides cbmai 857, penicillium raistrickii cbmai 931, penicillium citrinum cbma 1186, mucor racemosus cbmai 847, beauveria felina cbmai 738, and penicillium oxalicum cbmai 1185) and terrestrial fungus penicillium chrysogenum cbma1199 were screened as catalysts for the asymmetric reduction of α-keto azides 5-8 to their corresponding β-azidophenylethanols 9-12. the marine fungi showed prelog and anti-pr ... | 2015 | 26272428 |
| determination of lipolytic and proteolytic activities of mycoflora isolated from dry-cured teruel ham. | fungi play a key role in dry-cured ham production because of their lipolytic and proteolytic activities. in the present study, 74 fungal strains from dry-cured teruel hams and air chambers were tested for proteolytic and lipolytic activities, with a view to their possible use as starter cultures. lipolytic activity of fungi was studied against lauric, palmitic, stearic and oleic acids, whereas proteolytic activity was studied against casein and myosin. of the 74 fungal strains tested, most of th ... | 2015 | 26243949 |
| identification and control of moulds responsible for black spot spoilage in dry-cured ham. | the aims of this work were to identify moulds responsible for black spot spoilage in the drying and cellar stages of dry-cured ham processing and evaluate the effectiveness of preventive actions for controlling this alteration. four mould strains isolated from spoiled hams were identified by morphological characteristics and the its and β-tubulin sequencing. two of them were cladosporium oxysporum, one was c. cladosporioides and the remaining one was c. herbarum. these spoiling strains reproduce ... | 2016 | 27468139 |
| augmentation of a microbial consortium for enhanced polylactide (pla) degradation. | bioplastics are eco-friendly and derived from renewable biomass sources. innovation in recycling methods will tackle some of the critical issues facing the acceptance of bioplastics. polylactic acid (pla) is the commonly used and well-studied bioplastic that is presumed to be biodegradable. considering their demand and use in near future, exploration for microbes capable of bioplastic degradation has high potential. four pla degrading strains were isolated and identified as penicillium chrysogen ... | 2016 | 26843697 |
| fungi associated with rocks of the atacama desert: taxonomy, distribution, diversity, ecology and bioprospection for bioactive compounds. | this study assessed the diversity of cultivable rock-associated fungi from atacama desert. a total of 81 fungal isolates obtained were identified as 29 ascomycota taxa by sequencing different regions of dna. cladosporium halotolerans, penicillium chrysogenum and penicillium cf. citrinum were the most frequent species, which occur at least in four different altitudes. the diversity and similarity indices ranged in the fungal communities across the latitudinal gradient. the fisher-α index displaye ... | 2016 | 26235221 |
| production of 8,11-dihydroxy and 8-hydroxy unsaturated fatty acids from unsaturated fatty acids by recombinant escherichia coli expressing 8,11-linoleate diol synthase from penicillium chrysogenum. | hydroxy unsaturated fatty acids can be used as antimicrobial surfactants. 8,11-linoleate diol synthase (8,11-lds) catalyzes the conversion of unsaturated fatty acid to 8-hydroperoxy unsaturated fatty acid, and it is subsequently isomerized to 8,11-dihydroxy unsaturated fatty acid by the enzyme. the optimal reaction conditions of recombinant escherichia coli expressing penicillium chrysogenum 8,11-lds for the production of 8,11-dihydroxy-9,12(z,z)-octadecadienoic acid (8,11-dihode), 8,11-dihydrox ... | 2016 | 27997074 |
| characterization and expression of the antifungal protein from monascus pilosus and its distribution among various monascus species. | monascus species are traditionally used for food preservation. this study used the disc diffusion method to verify the antifungal activity of protein extracted from monascus pilosus bcrc38072 against 15 fungal pathogens. an antifungal protein, designated as mafp1, was successfully purified and confirmed through n-terminal sequencing. to further explore the antifungal gene, a mafp1 gene that is similar to that of pgafp from penicillium chrysogenum was cloned from m. pilosus bcrc38072. according t ... | 2016 | 26803705 |
| synthesis, spectral investigation (¹h, ¹³c) and anti-microbial screening of benzophenone imines. | new series of benzophenone imines with general formula ph2-c=nr; r = benzyl, 4-fluorobenzyl, naphthyl, phenyl, 4-nitrophenyl were synthesized by condensation of dichlorodiphenylmethane and different aromatic primary amines (1:1) those imines were characterized by different physiochemical and spectroscopic techniques like melting point, elemental analysis, ft-ir, multinuclear nmr (¹h, ¹³c). after characterization, imines were subjected to anti-microbial activities. all compounds showed promising ... | 2015 | 26639485 |
| identification and characterization of three penicillium chrysogenum α-l-arabinofuranosidases (pcabf43b, pcabf51c, and afq1) with different specificities toward arabino-oligosaccharides. | we previously described four α-l-arabinofuranosidases (abfs) secreted by penicillium chrysogenum 31b. here, we cloned the fifth and sixth genes (pcabf43b and pcabf51c) encoding the abfs pcabf43b and pcabf51c in this strain and overexpressed these genes in escherichia coli. the deduced amino acid sequences of pcabf43b and pcabf51c were highly similar to putative abfs belonging to glycoside hydrolase families 43 and 51, respectively. semiquantitative reverse transcription polymerase chain reaction ... | 2015 | 26002506 |
| biochemical characterization and overexpression of an endo-rhamnogalacturonan lyase from penicillium chrysogenum. | rhamnogalacturonan lyase (pcrgl4a) was purified from the culture supernatant of penicillium chrysogenum 31b. pcrgl4a optimal activity occurred between ph 7-8 and at 40 °c. conserved domain search analysis identified pcrgl4a as a member of polysaccharide lyase family 4. pcrgl4a contains two conserved catalytic and four conserved substrate-binding residues as determined by x-ray crystallography of the aspergillus aculeatus rg lyase. recombinant pcrgl4a (rpcrgl4a) expressed in escherichia coli demo ... | 2015 | 25666014 |
| oxidative dearomatisation: the key step of sorbicillinoid biosynthesis†electronic supplementary information (esi) available: containing all experimental details. see doi: 10.1039/c3sc52911hclick here for additional data file. | an fad-dependent monooxygenase encoding gene (sorbc) was cloned from penicillium chrysogenum e01-10/3 and expressed as a soluble protein in escherichia coli. the enzyme efficiently performed the oxidative dearomatisation of sorbicillin and dihydrosorbicillin to give sorbicillinol and dihydrosorbicillinol respectively. bioinformatic examination of the gene cluster surrounding sorbc indicated the presence of two polyketide synthase (pks) encoding genes designated sorba and sorbb. the gene sorba-en ... | 2014 | 25580210 |
| meleagrin, a new fabi inhibitor from penicillium chryosogenum with at least one additional mode of action. | bacterial enoyl-acyl carrier protein reductase (fabi) is a promising novel antibacterial target. we isolated a new class of fabi inhibitor from penicillium chrysogenum, which produces various antibiotics, the mechanisms of some of them are unknown. the isolated fabi inhibitor was determined to be meleagrin by mass spectroscopy and nuclear magnetic resonance spectral analyses, and its more active and inactive derivatives were chemically prepared. consistent with their selective inhibition of stap ... | 2013 | 24312171 |
| substrate specificity and gene expression of two penicillium chrysogenum α-l-arabinofuranosidases (afq1 and afs1) belonging to glycoside hydrolase families 51 and 54. | we previously isolated two α-l-arabinofuranosidases (abfs), termed afq1 and afs1, from the culture filtrate of penicillium chrysogenum 31b. afq1 and afs1 complementary dnas encoding afq1 and afs1 were isolated by in vitro cloning. the deduced amino acid sequences of afq1 and afs1 are highly similar to those of penicillium purpurogenum abf 2 and abf 1, respectively, which belong to glycoside hydrolase (gh) families 51 and 54, respectively. pfam analysis revealed an "alpha-l-af_c" domain in afq1 a ... | 2013 | 22410744 |
| alteration of protein patterns in black rock inhabiting fungi as a response to different temperatures. | rock inhabiting fungi are among the most stress tolerant organisms on earth. they are able to cope with different stressors determined by the typical conditions of bare rocks in hot and cold extreme environments. in this study first results of a system biological approach based on two-dimensional protein profiles are presented. protein patterns of extremotolerant black fungi -coniosporium perforans, exophiala jeanselmei - and of the extremophilic fungus -friedmanniomyces endolithicus - were comp ... | 2012 | 22862921 |
| halotolerant ability and α-amylase activity of some saltwater fungal isolates. | four halotolerant fungal isolates originating from the saltwater lake urmia in iran were selected during a screening program for salt resistance and α-amylase activity. the isolates were identified based on sequencing the its region and a part of the β-tubulin gene, as penicillium chrysogenum (isolate u1; cbs 132820), fusarium incarnatum (isolate u2; cbs 132821), and penicillium polonicum (isolate u3; cbs 132822, and isolate u4; cbs 132823). the growth of these isolates was determined by measuri ... | 2013 | 24250679 |
| vacuolar serine protease is a major allergen of fusarium proliferatum and an ige-cross reactive pan-fungal allergen. | fusarium species are among prevalent airborne fungi and causative agents of human respiratory atopic disorders. we previously identified a 36.5-kda f. proliferatum component recognized by ige antibodies in 9 (53%) of the 17 f. proliferatum-sensitized atopic serum samples. the purpose of this study is to characterize the 36.5-kda allergen of f. proliferatum. | 2016 | 27334782 |
| compost as a source of microbial isolates for the bioremediation of heavy metals: in vitro selection. | heavy metal pollution has become a major environmental concern nowadays and the bioremediation of polluted habitats is an increasingly popular strategy due to both its efficiency and safety. a screening and selection protocol based on different composting processes was designed in order to isolate heavy metal-resistant microorganisms. a collection of 51 microorganisms was obtained and most of them showed the capability to tolerate heavy metals in multi-polluted aqueous systems (cd(ii), cr(vi), n ... | 2012 | 22664539 |
| sorbicatechols a and b, antiviral sorbicillinoids from the marine-derived fungus penicillium chrysogenum pjx-17. | two novel sorbicillinoids combining a bicyclo[2.2.2]octane with a 2-methoxyphenol moiety, named sorbicatechols a (1) and b (2), were isolated from the culture of the marine sediment-derived fungus penicillium chrysogenum pjx-17, together with the known protocatechuic acid methyl ester and caffeic acid methyl ester (3). their structures, including absolute configurations, were assigned by analysis of nmr, ms data, and tddft ecd calculations. compounds 1 and 2 exhibited activities against influenz ... | 2014 | 24495078 |
| hepatitis c virus ns3-ns4a protease inhibitors from the endophytic penicillium chrysogenum isolated from the red alga liagora viscida. | hepatitis c virus (hcv) ns3-ns4a protease is an attractive target for anti-hcv agents because of its important role in replication. in this work, we demonstrated that the ethyl acetate extract of the endophytic fungus penicillium chrysogenum exhibited a potent activity against hcv ns3-ns4a protease with an ic50 value of 20 microg/ml. the fungus was isolated from the red alga liagora viscida and identified by its morphology and 18s rdna. large-scale fermentation of the fungus in czapek's peptone ... | 2014 | 24459769 |
| a new variant of self-excising β-recombinase/six cassette for repetitive gene deletion and homokaryon purification in neurospora crassa. | in a previous study, we developed a cassette employing a bacterial β-recombinase acting on six recognition sequences (β-rec/six), which allowed repetitive site-specific gene deletion and marker recycling in neurospora crassa. however, only one positive selection marker was used in the cassette. a tedious subsequent procedure was needed to purify homokaryons due to the lack of a negative selection after cassette eviction. additionally, the endoxylanase xylp promoter from penicillium chrysogenum u ... | 2014 | 24556286 |
| molecular cloning and expression of fungal cellobiose transporters and β-glucosidases conferring efficient cellobiose fermentation in saccharomyces cerevisiae. | cellobiose was once regarded as a byproduct that should be removed from biomass hydrolysates because of its inhibitory activity to cellulases. it was revealed, however, that cellobiose could serve as a co-substrate for xylose fermentation by engineered saccharomyces cerevisiae. despite its advantages, to date, little is known about cellodextrin transporters that endow s. cerevisiae with cellobiose transporting ability. in this study, engineered s. cerevisiae strains capable of fermenting cellobi ... | 2014 | 24184384 |
| cryo-em near-atomic structure of a dsrna fungal virus shows ancient structural motifs preserved in the dsrna viral lineage. | viruses evolve so rapidly that sequence-based comparison is not suitable for detecting relatedness among distant viruses. structure-based comparisons suggest that evolution led to a small number of viral classes or lineages that can be grouped by capsid protein (cp) folds. here, we report that the cp structure of the fungal dsrna penicillium chrysogenum virus (pcv) shows the progenitor fold of the dsrna virus lineage and suggests a relationship between lineages. cryo-em structure at near-atomic ... | 2014 | 24821769 |
| penicillium strains isolated from slovak grape berries taxonomy assessment by secondary metabolite profile. | the secondary metabolite profiles of microfungi of the genus penicillium isolated from samples of grape berries collected in two different phases during two vegetative seasons in slovakia is described to assess the taxonomy. three slovak vine regions have been selected for this study, based on their climatic differences and national economic importance. cultures of microfungi isolated from berries were incubated on different selective media for macro and micromorphology identification. the speci ... | 2014 | 25109845 |
| microscopic fungi recovered from honey and their toxinogenity. | the objective of this investigation was to contribute towards the knowledge of microbiology of honey, more than 50 samples of honey from slovakia and other countries were mycologically investigated in terms of the overall fungal diversity and toxicological potential of isolated fungi from penicillium genera. the study revealed that out of 13 genera recovered, penicillium was the most frequent and diverse genus, followed by aspergillus and cladosporium being found in 65.91 % (29 samples), 34.1 % ... | 2012 | 22702826 |
| [investigation of mold fungi in air samples of elementary schools and evaluation of allergen-specific ige levels in students' sera]. | atmospheric fungal spores play important role in allergic reactions in atopic individuals. monitorization of those spores found in the environment of atopic cases is crucial for the choice of the antigens that will be included in allergen screening procedures and precautions to be taken against mold-originated health problems. since most of the people spend plenty of time indoors in recent years, the effects of exposure to indoor air fungi on human health have gained importance. this study was a ... | 2012 | 22639315 |
| transcriptome analysis of the two unrelated fungal β-lactam producers acremonium chrysogenum and penicillium chrysogenum: velvet-regulated genes are major targets during conventional strain improvement programs. | cephalosporins and penicillins are the most frequently used β-lactam antibiotics for the treatment of human infections worldwide. the main industrial producers of these antibiotics are acremonium chrysogenum and penicillium chrysogenum, two taxonomically unrelated fungi. both were subjects of long-term strain development programs to reach economically relevant antibiotic titers. it is so far unknown, whether equivalent changes in gene expression lead to elevated antibiotic titers in production s ... | 2017 | 28359302 |
| a 9-pool metabolic structured kinetic model describing days to seconds dynamics of growth and product formation by penicillium chrysogenum. | a powerful approach for the optimization of industrial bioprocesses is to perform detailed simulations integrating large scale computational fluid dynamics (cfd) and cellular reaction dynamics (crd). however, complex metabolic kinetic models containing a large number of equations pose formidable challenges in cfd-crd coupling and computation time afterward. this necessitates to formulate a relatively simple but yet representative model structure. such a kinetic model should be able to reproduce ... | 2017 | 28322433 |
| purification, kinetic, and thermodynamic characteristics of an exo-polygalacturonase from penicillium notatum with industrial perspective. | an extracellular exo-polygalacturonase (exo-pg) produced by penicillium notatum was purified (3.07-folds) by ammonium sulfate fractionation, ion exchange, and gel filtration chromatography. two distinct isoforms of the enzyme, namely exo-pgi and exo-pgii, were identified during column purification with molecular weights of 85 and 20 kda, respectively, on sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page). the enzyme displayed its optimum activity at ph 6.0 and 50 °c and was fou ... | 2017 | 28316005 |
| penicillin production in industrial strain penicillium chrysogenum p2niad18 is not dependent on the copy number of biosynthesis genes. | multi-copy gene integration into microbial genomes is a conventional tool for obtaining improved gene expression. for penicillium chrysogenum, the fungal producer of the beta-lactam antibiotic penicillin, many production strains carry multiple copies of the penicillin biosynthesis gene cluster. this discovery led to the generally accepted view that high penicillin titers are the result of multiple copies of penicillin genes. here we investigated strain p2niad18, a production line that carries on ... | 2017 | 28209150 |
| structurally diverse secondary metabolites from a deep-sea-derived fungus penicillium chrysogenum scsio 41001 and their biological evaluation. | five new compounds, including a cytotoxic dimeric isocoumarin, bipenicilisorin (1), a merosesquiterpenoid, yaminterritrem c (2), a citrinin dimer, penicitrinone f (3), a alkaloid, terremide d (4), and a δ-valerolacton, (e)-4-(propen-1-yl)-5,6-dihydro-2h-pyran-2-one (5), along with ten known compounds (6-15) were isolated from a deep-sea-derived fungus penicillium chrysogenum scsio 41001. their structures and absolute configurations were elucidated by nmr spectra, ms, cd, optical rotation, x-ray ... | 2017 | 28108327 |
| improved catalytic properties of penicillium notatum lipase immobilized in nanoscale silicone polymeric films. | lipases are one of the most proficient biocatalysts having enormous biotechnological prospective. immobilization offers a potential solution to improve the stability and recycling characteristics of lipases. an extracellular lipase from penicillium notatum (pnl) was immobilized in silicon polymers (sip) through entrapment, and subsequently coated this matrix on the network of fibers in the sponges. the silicone polymers-immobilized lipase (sip-lipase) displayed highest apparent activity and entr ... | 2017 | 28089928 |
| d19s mutation of the cationic, cysteine-rich protein paf: novel insights into its structural dynamics, thermal unfolding and antifungal function. | the cysteine-rich, cationic, antifungal protein paf is abundantly secreted into the culture supernatant of the filamentous ascomycete penicillium chrysogenum. the five β-strands of paf form a compact β-barrel that is stabilized by three disulphide bonds. the folding of paf allows the formation of four surface-exposed loops and distinct charged motifs on the protein surface that might regulate the interaction of paf with the sensitive target fungus. the growth inhibitory activity of this highly s ... | 2017 | 28072824 |
| casein phosphopeptides and cacl2 increase penicillin production and cause an increment in microbody/peroxisome proteins in penicillium chrysogenum. | transport of penicillin intermediates and penicillin secretion are still poorly characterized in penicillium chrysogenum (re-identified as penicillium rubens). calcium (ca(2+)) plays an important role in the metabolism of filamentous fungi, and casein phosphopeptides (cpp) are involved in ca(2+) internalization. in this study we observe that the effect of cacl2 and cpp is additive and promotes an increase in penicillin production of up to 10-12 fold. combination of cacl2 and cpp greatly promotes ... | 2017 | 28062375 |
| evaluation of hdpe and ldpe degradation by fungus, implemented by statistical optimization. | plastic in any form is a nuisance to the well-being of the environment. the 'pestilence' caused by it is mainly due to its non-degradable nature. with the industrial boom and the population explosion, the usage of plastic products has increased. a steady increase has been observed in the use of plastic products, and this has accelerated the pollution. several attempts have been made to curb the problem at large by resorting to both chemical and biological methods. chemical methods have only resu ... | 2017 | 28051105 |
| improvement of activity, thermo-stability and fruit juice clarification characteristics of fungal exo-polygalacturonase. | an extracellular exo-polygalacturonase (exo-pg) from penicillium notatum was immobilized in sodium-alginate matrix through two different protocols, viz. covalent bonding and adsorption to enhance its catalytic activity, thermal stability and life-time properties for industrial applications. covalent immobilization was more efficient in terms of high relative activity (45.89%) and immobilization yield (71.6%) as compared to adsorption. immobilized exo-pg derivatives displayed maximum activities a ... | 2017 | 27984141 |
| biodegradation of waste grease by penicillium chrysogenum for production of fatty acid. | the aim of present work was to effectively remediate grease waste by penicillium chrysogenum. for efficient degradation, grease waste was pre-treated using various lipases, among them lipolase was the best. the pretreated grease was used as a substrate by p. chrysogenum resulting into the production of fatty acids. process was optimized by response surface methodology (rsm) using four variables viz; fecl2 (mm), spore concentration (spores/ml), time period (days) and amount of grease (g). the opt ... | 2017 | 27978437 |
| euler-lagrange computational fluid dynamics for (bio)reactor scale down: an analysis of organism lifelines. | the trajectories, referred to as lifelines, of individual microorganisms in an industrial scale fermentor under substrate limiting conditions were studied using an euler-lagrange computational fluid dynamics approach. the metabolic response to substrate concentration variations along these lifelines provides deep insight in the dynamic environment inside a large-scale fermentor, from the point of view of the microorganisms themselves. we present a novel methodology to evaluate this metabolic res ... | 2016 | 27917102 |
| degradation of benzo[a]pyrene by pleurotus ostreatus po-3 in the presence of defined fungal and bacterial co-cultures. | benzo[a]pyrene, a high molecular weight polycyclic aromatic hydrocarbon possesses carcinogenic, teratogenic, and mutagenic properties. the present study focuses on benzo[a]pyrene degradation by pleurotus ostreatus po-3, characterization and identification of metabolites produced and the extent of degradation in the presence of axenic culture of p. ostreatus po-3 and defined co-cultures of the basidiomycete with bacteria and non-basidiomycete fungi. thin-layer chromatography revealed that p. ostr ... | 2017 | 27874973 |
| comparison of fiber optic and conduit attenuated total reflection (atr) fourier transform infrared (ft-ir) setup for in-line fermentation monitoring. | the performance of a fiber optic and an optical conduit in-line attenuated total reflection mid-infrared (ir) probe during in situ monitoring of penicillium chrysogenum fermentation were compared. the fiber optic probe was connected to a sealed, portable, fourier transform infrared (ft-ir) process spectrometer via a plug-and-play interface. the optical conduit, on the other hand, was connected to a ft-ir process spectrometer via a knuckled probe with mirrors that had to be adjusted prior to each ... | 2016 | 27864445 |
| identification of fungal candidates for asthma protection in a large population-based study. | exposure to molds has been related to asthma risk both positively and negatively, depending on the environmental setting. the pertinent results are based on generic markers or culturing methods although the majority of present fungi cannot be cultured under laboratory conditions. the aim of the present analysis was to assess environmental dust samples for asthma-protective fungal candidates with a comprehensive molecular technique covering also non-cultivable and non-viable fungi. | 2017 | 27711990 |
| microbial transformation of the antimalarial sesquiterpene endoperoxide dihydroartemisinin. | dihydroartemisinin (dha, 1), a sesquiterpene endoperoxide derived from artemisinin, has shown potent antimalarial and anticancer activities. microbial transformation of dha by absidia coerulea and penicillium chrysogenum yielded one new (3) and four known metabolites (2, 4-6). the chemical structures of these compounds were identified as deoxydihydroartemisinin (2), 8α-hydroxydeoxyartemisinin (3), deoxyartemisinin (4), 9α-hydroxyartemethin-i (5) and 3α-hydroxydeoxydihydroartemisinin (6) using sp ... | 2017 | 27788595 |
| disseminated penicilliosis due to penicillium chrysogenum in a pediatric patient with henoch-schönlein syndrome. | a case of disseminated infection caused by penicillium chrysogenum in a 10-year-old boy with a history of henoch-schönlein purpura and proliferative glomerulonephritis, treated with immunosuppressors, is reported herein. the patient had a clinical picture of 2 weeks of fever that did not respond to treatment with broad-spectrum antibiotics and amphotericin b. computed tomography imaging showed diffuse cotton-like infiltrates in the lungs, hepatomegaly, mesenteric lymphadenopathy, and multiple we ... | 2016 | 27596684 |
| proteomic analysis of the signaling pathway mediated by the heterotrimeric gα protein pga1 of penicillium chrysogenum. | the heterotrimeric gα protein pga1-mediated signaling pathway regulates the entire developmental program in penicillium chrysogenum, from spore germination to the formation of conidia. in addition it participates in the regulation of penicillin biosynthesis. we aimed to advance the understanding of this key signaling pathway using a proteomics approach, a powerful tool to identify effectors participating in signal transduction pathways. | 2016 | 27716202 |
| hot off the press. | a personal selection of 32 recent papers is presented covering various aspects of current developments in bioorganic chemistry and novel natural products such as chrysamide a from a deep-sea fungus penicillium chrysogenum. | 2016 | 27714022 |
| bioactive chaetoglobosins from the mangrove endophytic fungus penicillium chrysogenum. | a novel chaetoglobosin named penochalasin i (1) with a unprecedented six-cyclic 6/5/6/5/6/13 fused ring system, and another new chaetoglobosin named penochalasin j (2), along with chaetoglobosins g, f, c, a, e, armochaetoglobosin i, and cytoglobosin c (3-9) were isolated from the culture of penicillium chrysogenum v11. their structures were elucidated by 1d, 2d nmr spectroscopic analysis and high resolution mass spectroscopic data. the absolute configuration of compounds 1 and 2 were determined ... | 2016 | 27690061 |
| genome-wide chromatin immunoprecipitation sequencing analysis of the penicillium chrysogenum velvet protein pcvela identifies methyltransferase pcllma as a novel downstream regulator of fungal development. | penicillium chrysogenum is the sole industrial producer of the β-lactam antibiotic penicillin, which is the most commonly used drug for treating bacterial infections. in p. chrysogenum and other filamentous fungi, secondary metabolism and morphogenesis are controlled by the highly conserved multisubunit velvet complex. here we present the first chromatin immunoprecipitation next-generation sequencing (chip-seq) analysis of a fungal velvet protein, providing experimental evidence that a velvet ho ... | 2017 | 27570838 |
| quantification of the fungal fraction released from various preloaded fibrous filters during a simulated ventilation restart. | this study aimed to demonstrate that particles, especially those associated with fungi, could be released from fibrous filters used in the air-handling unit (ahu) of heating, ventilation and air-conditioning (hvac) systems during ventilation restarts. quantification of the water retention capacity and sem pictures of the filters was used to show the potential for fungal proliferation in unused or preloaded filters. five fibrous filters with various particle collection efficiencies were studied: ... | 2016 | 27564375 |
| risk element sorption/desorption characteristics of dry olive residue: a technique for the potential immobilization of risk elements in contaminated soils. | olive oil production is one of the most relevant agroindustrial activities in the mediterranean region and generates a huge amount of both solid and semi-solid wastes, the uncontrolled disposal of which might lead to serious environmental problems. due to its organic matter and mineral nutrient content, the waste material can be applied to agricultural soil as a fertilizer. however, due to its high organic matter content, dry olive residue (dor), commonly called "alperujo," has the potential to ... | 2016 | 27557964 |
| at-line determining spore germination of penicillium chrysogenum bioprocesses in complex media. | spore inoculum quality in filamentous bioprocesses is a critical parameter associated with viable spore concentration (1) and spore germination (2). it influences pellet morphology and, consequently, process performance. the state-of-the-art method to measure viable spore concentration is tedious, associated with significant inherent bias, and not applicable in real-time. therefore, it is not usable as process analytical technology (pat). spore germination has so far been monitored using image a ... | 2016 | 27557717 |
| identification of the main regulator responsible for synthesis of the typical yellow pigment produced by trichoderma reesei. | the industrially used ascomycete trichoderma reesei secretes a typical yellow pigment during cultivation, while other trichoderma species do not. a comparative genomic analysis suggested that a putative secondary metabolism cluster, containing two polyketide-synthase encoding genes, is responsible for the yellow pigment synthesis. this cluster is conserved in a set of rather distantly related fungi, including acremonium chrysogenum and penicillium chrysogenum in an attempt to silence the cluster ... | 2016 | 27520818 |
| effect of dry mycelium of penicillium chrysogenum fertilizer on soil microbial community composition, enzyme activities and snap bean growth. | the dry mycelium fertilizer (dmf) was produced from penicillin fermentation fungi mycelium (pffm) following an acid-heating pretreatment to degrade the residual penicillin. in this study, it was applied into soil as fertilizer to investigate its effects on soil properties, phytotoxicity, microbial community composition, enzyme activities, and growth of snap bean in greenhouse. as the results show, ph, total nitrogen, total phosphorus, total potassium, and organic matter of soil with dmf treatmen ... | 2016 | 27473622 |
| synthesis and antimicrobial activity of styryl/pyrrolyl/pyrazolyl sulfonylmethyl-1,3,4-oxadiazolyl amines and styryl/pyrrolyl/pyrazolyl sulfonylmethyl-1,3,4-thiadiazolyl amines. | a new class of mono and bis heterocycles - styryl sulfonylmethyl-1,3,4-oxadiazolyl/1,3,4-thiadiazolyl amines, pyrrolyl sulfonylmethyl-1,3,4-oxadiazolyl/1,3,4-thiadiazolyl amines and pyrazolyl sulfonylmethyl-1,3,4-oxadiazolyl/1,3,4-thiadiazolyl amines were prepared from the synthetic intermediate z-styrylsulfonylacetic acid adopting simple and well versed synthetic methodologies and studied their antimicrobial activity. amongst all the tested compounds styryl thiadiazole 5c exhibited promising an ... | 2016 | 27448921 |
| chrysamides a-c, three dimeric nitrophenyl trans-epoxyamides produced by the deep-sea-derived fungus penicillium chrysogenum scsio41001. | three dimeric nitrophenyl trans-epoxyamides, chrysamides a-c (1-3), were obtained from the deep-sea-derived fungus penicillium chrysogenum scsio41001. their structures were characterized by spectroscopic analysis, electronic circular dichroism computations, and x-ray single-crystal diffraction analysis. notably, compound 1 possesses a novel centrosymmetric dimer skeleton featuring an unprecedented 7-oxa-2,5-diazabicyclo[2.2.1]heptane ring system, which represents the first example of dimeric nit ... | 2016 | 27440074 |
| mixed pulmonary infection with penicillium notatum and pneumocystis jiroveci in a patient with acute myeloid leukemia. | penicillium notatum is a fungus that widely exists in the environment and is often non-pathogenic to humans. however, in immunocompromised hosts it may be recognized as a cause of systemic mycosis. a 44-year-old man with acute myeloid leukemia (aml) was admitted to our hospital with fever and neutropenia. due to no improvement after initial treatment, he underwent bronchoscopy. the patient was found to have p. notatum and pneumocystis jiroveci infection, and therefore was given voriconazole, pri ... | 2016 | 27403180 |
| autochthonous microbial community associated with pine needle forest litterfall influences its degradation under natural environmental conditions. | the slow natural degradation of chir pine (pinus roxburghii) needle litterfall and its accumulation on forest floors have been attributed to its lignocellulosic complexities of the biomass. the present study offers a microbiological insight into the role of autochthonous microflora associated with pine needle litterfall in its natural degradation. the denaturing gradient gel electrophoresis (dgge) fingerprinting indicated actinomycetes (saccharomonospora sp., glycomyces sp., agrococcus sp., leif ... | 2016 | 27317052 |
| soluble material secreted from penicillium chrysogenum isolate exhibits antifungal activity against cryphonectria parasitica- the causative agent of the american chestnut blight. | the american chestnut (castanea dentata) was once the dominant canopy tree along the eastern region of the united states. cryphonectria parasitica, the causative agent of chestnut blight, was introduced from asia in the early 1900's, and obliterated the chestnut population within 50 years. we sought to identify environmental microbes capable of producing factors that were fungicidal or inhibited growth of c. parasitica in the hopes developing a biological control of chestnut blight. we isolated ... | 2016 | 27274909 |
| identification of a polyketide synthase involved in sorbicillin biosynthesis by penicillium chrysogenum. | secondary metabolism in penicillium chrysogenum was intensively subjected to classical strain improvement (csi), the resulting industrial strains producing high levels of β-lactams. during this process, the production of yellow pigments, including sorbicillinoids, was eliminated as part of a strategy to enable the rapid purification of β-lactams. here we report the identification of the polyketide synthase (pks) gene essential for sorbicillinoid biosynthesis in p. chrysogenum we demonstrate that ... | 2016 | 27107123 |
| genetic and morphological diversity of the genus penicillium from mazandaran and tehran provinces, iran. | the genus penicillium contains a large number of ubiquitous environmental taxa, of which some species are clinically important. identification of penicillium down to the species level is currently based on polyphasic criteria, including phenotypic features and genetic markers. biodiversity of the genus penicillium from mazandaran and tehran provinces has not been described. | 2016 | 27099684 |