| conformational and functional studies of a cytosolic 90 kda heat shock protein hsp90 from sugarcane. | hsp90s are involved in several cellular processes, such as signaling, proteostasis, epigenetics, differentiation and stress defense. although hsp90s from different organisms are highly similar, they usually have small variations in conformation and function. thus, the characterization of different hsp90s is important to gain insight into the structure-function relationship that makes these chaperones key regulators in protein homeostasis. this work describes the characterization of a cytosolic h ... | 2013 | 23619240 |
| global analysis of the sugarcane microtranscriptome reveals a unique composition of small rnas associated with axillary bud outgrowth. | axillary bud outgrowth determines shoot architecture and is under the control of endogenous hormones and a fine-tuned gene-expression network, which probably includes small rnas (srnas). although it is well known that srnas act broadly in plant development, our understanding about their roles in vegetative bud outgrowth remains limited. moreover, the expression profiles of micrornas (mirnas) and their targets within axillary buds are largely unknown. here, we employed srna next-generation sequen ... | 2013 | 23564956 |
| mapping the lignin distribution in pretreated sugarcane bagasse by confocal and fluorescence lifetime imaging microscopy. | delignification pretreatments of biomass and methods to assess their efficacy are crucial for biomass-to-biofuels research and technology. here, we applied confocal and fluorescence lifetime imaging microscopy (flim) using one- and two-photon excitation to map the lignin distribution within bagasse fibers pretreated with acid and alkali. the evaluated spectra and decay times are correlated with previously calculated lignin fractions. we have also investigated the influence of the pretreatment on ... | 2013 | 23548159 |
| high-throughput sequencing of small rna transcriptome reveals salt stress regulated micrornas in sugarcane. | salt stress is a primary cause of crop losses worldwide, and it has been the subject of intense investigation to unravel the complex mechanisms responsible for salinity tolerance. microrna is implicated in many developmental processes and in responses to various abiotic stresses, playing pivotal roles in plant adaptation. deep sequencing technology was chosen to determine the small rna transcriptome of saccharum sp cultivars grown on saline conditions. we constructed four small rnas libraries pr ... | 2013 | 23544066 |
| biochemical biomarkers in scinax fuscovarius tadpoles exposed to a commercial formulation of the pesticide fipronil. | one of the main pesticides used in the cultivation of sugarcane in são paulo state, brazil, is regent(®)800wg, the main active compound of which is fipronil. fipronil is a potent insecticide that eliminates pests, including insects resistant to pyrethroids, organophosphates (op) and carbamates (ca). there is little known on the toxic effects of fipronil on non-target organisms, such as tadpoles of frogs. it is possible that this compound carries a high toxicity for these organisms, since the pes ... | 2013 | 23489839 |
| study of kinetic parameters in a mechanistic model for enzymatic hydrolysis of sugarcane bagasse subjected to different pretreatments. | the goal of this work is to evaluate the influence of different pretreatments in the kinetics of enzymatic hydrolysis of sugarcane bagasse and to propose a reliable methodology to easily perform sensitivity analysis and updating kinetic parameters whenever necessary. a kinetic model was modified to represent the experimental data of the batch enzymatic hydrolysis of sugarcane bagasse pretreated with alkaline hydrogen peroxide. the simultaneous estimation of kinetic parameters of the mathematical ... | 2013 | 23474967 |
| expression of soflac, a new laccase in sugarcane, restores lignin content but not s:g ratio of arabidopsis lac17 mutant. | lignin is a complex phenolic heteropolymer deposited in the secondarily thickened walls of specialized plant cells to provide strength for plants to stand upright and hydrophobicity to conducting cells for long-distance water transport. although essential for plant growth and development, lignin is the major plant cell-wall component responsible for biomass recalcitrance to industrial processing. peroxidases and laccases are generally thought to be responsible for lignin polymerization, but, giv ... | 2013 | 23418623 |
| enterobacter sacchari sp. nov., a nitrogen-fixing bacterium associated with sugar cane (saccharum officinarum l.). | five nitrogen-fixing bacterial strains (sp1(t), nn143, nn144, nn208 and hx148) were isolated from stem, root or rhizosphere soil of sugar cane (saccharum officinarum l.) plants. cells were gram-negative, motile, rods with peritrichous flagella. dna g+c content was 55.0 ± 0.5 mol%. sequence determinations and phylogenetic analysis of 16s rrna gene and rpob indicated that the strains were affiliated with the genus enterobacter and most closely related to e. radicincitans dsm 16656(t) and e. oryzae ... | 2013 | 23291881 |
| bioconversion of sugarcane biomass into ethanol: an overview about composition, pretreatment methods, detoxification of hydrolysates, enzymatic saccharification, and ethanol fermentation. | depleted supplies of fossil fuel, regular price hikes of gasoline, and environmental damage have necessitated the search for economic and eco-benign alternative of gasoline. ethanol is produced from food/feed-based substrates (grains, sugars, and molasses), and its application as an energy source does not seem fit for long term due to the increasing fuel, food, feed, and other needs. these concerns have enforced to explore the alternative means of cost competitive and sustainable supply of biofu ... | 2012 | 23251086 |
| x-ray crystallography and nmr studies of domain-swapped canecystatin-1. | the three-dimensional structure of canecystatin-1, a potent inhibitor of cysteine proteases from sugarcane (saccharum officinarum), has been solved in two different crystal forms. in both cases, it is seen to exist as a domain-swapped dimer, the first such observation for a cystatin of plant origin. size exclusion chromatography and multidimensional nmr spectroscopy show the dimer to be the dominant species in solution, despite the presence of a measurable quantity of monomer undergoing slow exc ... | 2013 | 23241243 |
| correlation between agar plate screening and solid-state fermentation for the prediction of cellulase production by trichoderma strains. | the viability of converting biomass into biofuels and chemicals still requires further development towards the reduction of the enzyme production costs. thus, there is a growing demand for the development of efficient procedures for selection of cellulase-producing microorganisms. this work correlates qualitative screening using agar plate assays with quantitative measurements of cellulase production during cultivation under solid-state fermentation (ssf). the initial screening step consisted of ... | 2012 | 23227312 |
| genome sequence of enterobacter sp. strain sp1, an endophytic nitrogen-fixing bacterium isolated from sugarcane. | enterobacter sp. strain sp1 is an endophytic nitrogen-fixing bacterium isolated from a sugarcane stem and can promote plant growth. the draft genome sequence of strain sp1 presented here will promote comparative genomic studies to determine the genetic background of interactions between endophytic enterobacteria and plants. | 2012 | 23209221 |
| enzymatic saccharification of sugarcane bagasse by n-methylmorpholine-n-oxide-tolerant cellulase from a newly isolated galactomyces sp. cczu11-1. | based on the enrichment culture strategy, a novel n-methylmorpholine-n-oxide (nmmo)-tolerant cellulase-producing strain galactomyces sp. cczu11-1 was isolated from soil samples. after the optimization of culture condition, the highest fpa (13.4 u/ml) and cmcase (24.5 u/ml) were obtained. in both culture and reaction media containing nmmo 25% (w/v), the cellulase from galactomyces sp. cczu11-1 still had good activity. furthermore, high saccharification rate was obtained in aqueous-nmmo media. mor ... | 2013 | 23186661 |
| suspension cell culture as a tool for the characterization of class iii peroxidases in sugarcane. | secreted class iii peroxidases (ec 1.11.1.7) are implicated in a broad range of physiological processes throughout the plant life cycle. however, the unambiguous determination of the precise biological role of an individual class iii peroxidase isoenzyme is still a difficult task due to genetic redundancy and broad substrate specificity in vitro. in addition, many difficulties are encountered during extraction and analysis of cell wall proteins. since class iii peroxidases are also secreted into ... | 2013 | 23159486 |
| short-term usage of sewage sludge as organic fertilizer to sugarcane in a tropical soil bears little threat of heavy metal contamination. | a field experiment was carried out to study the effect of application rates of sewage sludge and mineral nitrogen and phosphate fertilizers on as, ba, cd, cr, cu, ni, pb, se, and zn concentration in soil, cane plant, and first ratoon (residual effect) in a typic hapludult soil. to allow an analysis by means of response surface modeling, four rates of sewage sludge (0, 3.6, 7.2 and 10.8 t ha(-1), dry base), of n (0, 30, 60 and 90 kg ha(-1)) and of p(2)o(5) (0, 60, 120 and 180 kg ha(-1)) were appl ... | 2013 | 23137916 |
| effects of drought on the microtranscriptome of field-grown sugarcane plants. | sugarcane (saccharum spp.) is the most promising crop for renewable energy. among the diverse stresses that affect plant productivity, drought stress frequently causes losses in sugarcane fields. although several studies have addressed plant responses to drought using controlled environments, plant responses under field conditions are largely unknown. recently, microrna (mirna)-mediated post-transcriptional regulation has been described as an important and decisive component in vegetal developme ... | 2013 | 23129215 |
| butanol production in a first-generation brazilian sugarcane biorefinery: technical aspects and economics of greenfield projects. | the techno-economics of greenfield projects of a first-generation sugarcane biorefinery aimed to produce ethanol, sugar, power, and n-butanol was conducted taking into account different butanol fermentation technologies (regular microorganism and mutant strain with improved butanol yield) and market scenarios (chemicals and automotive fuel). the complete sugarcane biorefinery with the batch acetone-butanol-ethanol (abe) fermentation process was simulated using aspen plus®. the biorefinery was de ... | 2013 | 23127845 |
| functional characterization of sugarcane mustang domesticated transposases and comparative diversity in sugarcane, rice, maize and sorghum. | transposable elements (tes) account for a large portion of plant genomes, particularly in grasses, in which they correspond to 50%-80% of the genomic content. tes have recently been shown to be a source of new genes and new regulatory networks. the most striking contribution of tes is referred as "molecular domestication", by which the element coding sequence loses its movement capacity and acquires cellular function. recently, domesticated transposases known as mustang and derived from the muta ... | 2012 | 23055803 |
| whole-genome sequence of enterobacter sp. strain sst3, an endophyte isolated from jamaican sugarcane (saccharum sp.) stalk tissue. | enterobacter sp. strain sst3 is an endophytic bacterium isolated from saccharum spp. here we present its annotated draft genome that may shed light on its role as a bacterial endophyte of sugarcane. to our knowledge, this is the first genome announcement of a sugarcane-associated bacterium from the genus enterobacter. | 2012 | 23045495 |
| a review on soil carbon accumulation due to the management change of major brazilian agricultural activities. | agricultural areas deal with enormous co2 intake fluxes offering an opportunity for greenhouse effect mitigation. in this work we studied the potential of soil carbon sequestration due to the management conversion in major agricultural activities in brazil. data from several studies indicate that in soybean/maize, and related rotation systems, a significant soil carbon sequestration was observed over the year of conversion from conventional to no-till practices, with a mean rate of 0.41 mg c ha( ... | 2012 | 23011303 |
| using population genetic methods to identify the origin of an invasive population and to diagnose cryptic subspecies of telchin licus (lepidoptera: castniidae). | telchin licus, the giant sugarcane borer, is an important pest species of sugarcane in northeast brazil. four subspecies of telchin licus are recognized in brazil based on their geographic distribution and subtle differences in wing colour pattern. some taxa are morphologically indistinguishable, and their accurate identification is key to their efficient control. mitochondrial genes sequences (cytochrome oxidase i and subunit 6 of the nicotinamide adenine dinucleotide dehydrogenase) were applie ... | 2013 | 22971459 |
| acetobacter okinawensis sp. nov., acetobacter papayae sp. nov., and acetobacter persicus sp. nov.; novel acetic acid bacteria isolated from stems of sugarcane, fruits, and a flower in japan. | eleven strains of acetic acid bacteria were isolated from stems of sugarcane, fruits, and a flower in japan. the isolates were separated into three groups, groups i, ii, and iii, in the genus acetobacter according to phylogenetic analysis based on 16s rrna sequences. the isolates had sequence similarities of 99.8-100% within the group, 99.3-99.6% to those of the type strains of each related acetobacter species, and less than 98.4% to those of the type strains of other acetobacter species. genomi ... | 2012 | 22878741 |
| concentrations of cu, fe, mn, and zn in tropical soils amended with sewage sludge and composted sewage sludge. | sewage sludge may be used as an agricultural fertilizer, but the practice has been criticized because sludge may contain trace elements and pathogens. the aim of this study was to compare the effectiveness of total and pseudototal extractants of cu, fe, mn, and zn, and to compare the results with the bioavailable concentrations of these elements to maize and sugarcane in a soil that was amended with sewage sludge for 13 consecutive years and in a separate soil that was amended a single time with ... | 2013 | 22810380 |
| functional characterization and synergic action of fungal xylanase and arabinofuranosidase for production of xylooligosaccharides. | plant cell wall degrading enzymes are key technological components in biomass bioconversion platforms for lignocellulosic materials transformation. cost effective production of enzymes and identification of efficient degradation routes are two economic bottlenecks that currently limit the use of renewable feedstocks through an environmental friendly pathway. the present study describes the hypersecretion of an endo-xylanase (gh11) and an arabinofuranosidase (gh54) by a fungal expression system w ... | 2012 | 22750495 |
| recombinant expression and biochemical characterization of sugarcane legumain. | plant legumains, also termed vacuolar processing enzymes (vpes), are cysteine peptidases that play key roles in plant development, senescence, programmed cell death and defense against pathogens. despite the increasing number of reports on plant cysteine peptidases, including vpes, the characterization of sugarcane vpes and their inhibition by endogenous cystatins have not yet been described. this is the first report of the biochemical characterization of a sugarcane cysteine peptidase. in this ... | 2012 | 22721948 |
| a novel multienzyme complex from a newly isolated facultative anaerobic bacterium, paenibacillus sp. tw1. | a multienzyme complex from newly isolated paenibacillus sp. tw1 was purified from pellet-bound enzyme preparations by elution with 0.25% sucrose and 1.0% triethylamine (tea), ultrafiltration and sephacryl s-400 gel filtration chromatography. the purified multienzyme complex showed a single protein band on non-denaturing polyacrylamide gel electrophoresis (native-page). the high molecular mass of the purified multienzyme complex was approximately 1,950 kda. the complex consisted of xylanase and c ... | 2012 | 22695526 |
| identification of sense and antisense transcripts regulated by drought in sugarcane. | sugarcane is an important sugar and energy crop that can be used efficiently for biofuels production. the development of sugarcane cultivars tolerant to drought could allow for the expansion of plantations to sub-prime regions. knowledge on the mechanisms underlying drought responses and its relationship with carbon partition would greatly help to define routes to increase yield. in this work we studied sugarcane responses to drought using a custom designed oligonucleotide array with 21,901 diff ... | 2012 | 22610347 |
| influence of sugarcane burning on indoor/outdoor pah air pollution in brazil. | this work presents the influence of sugarcane burning on pah levels and their profiles at a residence located in araraquara (sp, brazil), a city surrounded by sugarcane plantations. the average concentrations of total pahs (σpahs) associated with atmospheric particulate matter were higher during the burning period (σpahs 22.9 ng m(-3)) than in the non-burning period (σpah 2.35 ng m(-3)). a comparison of our results with previous studies regarding pah levels and their profiles in araraquara outdo ... | 2012 | 22578797 |
| establishment of an in vitro regeneration system for genetic transformation of selected sugarcane genotypes. | a good culture system provides considerable quantities of highly regenerable target tissues. embryogenic callus cultures are ideal for micro-projectile-mediated transformation, because regenerable cells are not very stable. effective exploitation of genetic transformation requires good regeneration systems. we selected three sugarcane genotypes for the establishment and optimization of good in vitro regeneration systems, viz., s-2003-us-359, s-2006-sp-30, and s-2003-us-165. three callus inductio ... | 2012 | 22535387 |
| a bac library of the sp80-3280 sugarcane variety (saccharum sp.) and its inferred microsynteny with the sorghum genome. | sugarcane breeding has significantly progressed in the last 30 years, but achieving additional yield gains has been difficult because of the constraints imposed by the complex ploidy of this crop. sugarcane cultivars are interspecific hybrids between saccharum officinarum and saccharum spontaneum. s. officinarum is an octoploid with 2n = 80 chromosomes while s. spontaneum has 2n = 40 to 128 chromosomes and ploidy varying from 5 to 16. the hybrid genome is composed of 70-80% s. officinaram and 5- ... | 2012 | 22524198 |
| mass balance of pilot-scale pretreatment of sugarcane bagasse by steam explosion followed by alkaline delignification. | five pilot-scale steam explosion pretreatments of sugarcane bagasse followed by alkaline delignification were explored. the solubilised lignin was precipitated with 98% sulphuric acid. most of the pentosan (82.6%), and the acetyl group fractions were solubilised during pretreatment, while 90.2% of cellulose and 87.0% lignin were recovered in the solid fraction. approximately 91% of the lignin and 72.5% of the pentosans contained in the steam-exploded solids were solubilised by delignification, r ... | 2012 | 22391588 |
| production of a his-tagged canecystatin in transgenic sugarcane. | transgenic plants have been widely used as expression systems of recombinant proteins in recent years because it can be an efficient alternative for the large-scale production of proteins. this is an area with great potential but is still not much explored. indeed, this system can bring a breakthrough in the expression of any protein. the model used here as a protein factory was sugarcane, a crop of great global importance. this chapter describes the system that has been adopted in the routine p ... | 2012 | 22351027 |
| study of postures in sugarcane cutters in the pontal of paranapanema-sp, brazil. | the expansion of sugarcane monoculture in brazil in the last decades has pointed out to the necessity of considering the question of sugarcane cutters occupational health. in this work we present a cross-sectional study aiming to examine the occupational posture of a group of sugarcane cutters, which work in a cane field located in the region of pontal do paranapanema- sp, brazil. the study was made using the ergonomic analysis of work - eaw methodology and the postural analysis method by win-ow ... | 2012 | 22317560 |
| impacts of introducing a new tool for the manual harvesting of sugar cane: the ergonomic analysis contribution. | the purpose of this study was to evaluate the impact of introduction a new work tool on health and productivity of manual harvesting sugarcane workers. in this study it was performed aergonomic work analysis (ewa) to analyze the work tool changes.the study included sugarcane workers, drivers, fiscals, and technical's safety at work.during the analysis process, 134 questionnaires were applied,aimed to identify the constraints that such workers are submitted,to evaluate the tool introduced, in ord ... | 2012 | 22317488 |
| protective gloves on manual sugar cane cutting are really effective? | problems related to the use of personal protective equipment (ppe), specially the use of protective gloves for the manual sugar cane cutting, motivated this research, made possible by a tripartite negotiation involving the ministry of labor, the union of rural workers and the employer's association of sugarcane agribusiness. the main objective was to evaluate, from an ergonomics perspective, the impact of use of the gloves during the manual cane sugar cutting, raising questions on safety, effect ... | 2012 | 22317487 |
| biocatalytic potential of an alkalophilic and thermophilic dextranase as a remedial measure for dextran removal during sugar manufacture. | the present study is focused on dextranase from streptomyces sp. nk458 with potential to remove dextran formed during sugar manufacture. the dextranase had molecular weight of 130 kda and hydrolyzed 15-25 and 410 kda dextran. dextranase production was optimized using statistical designs and the enzyme was purified 1.8-fold with 55.5% recovery. it displayed maximum activity at ph 9.0 and 60°c and was stable over a wide range of ph from 5.0 to 10.0. the k(m) and v(max) values were 3.05 mm and 17.9 ... | 2012 | 22277209 |
| [men of the sugarcane fields and their hospitals: the architecture of health under the estado novo]. | the article explores the emergence of an architectural heritage in the realm of healthcare assistance for workers in the sugarcane agroindustry in brazil following enactment of the law known as the estatuto da lavoura canavieira (1941), under the auspices of the instituto do açúcar e do álcool and as part of estado novo policies (1937-1945). the institute proposed solutions based on surveys conducted at sugarcane mills in cane-producing states and on the medical and hospital system adopted by th ... | 2011 | 22240948 |
| diversification of hat transposase paralogues in the sugarcane genome. | transposons are abundant components of eukaryotic genomes, and play important role in genome evolution. the knowledge about these elements should contribute to the understanding of their impact on the host genomes. the hat transposon superfamily is one of the best characterized superfamilies in diverse organisms, nevertheless, a detailed study of these elements was never carried in sugarcane. to address this question we analyzed 32 cdnas similar to that of hat superfamily of transposons previous ... | 2012 | 22228195 |
| a mixed model qtl analysis for sugarcane multiple-harvest-location trial data. | sugarcane-breeding programs take at least 12 years to develop new commercial cultivars. molecular markers offer a possibility to study the genetic architecture of quantitative traits in sugarcane, and they may be used in marker-assisted selection to speed up artificial selection. although the performance of sugarcane progenies in breeding programs are commonly evaluated across a range of locations and harvest years, many of the qtl detection methods ignore two- and three-way interactions between ... | 2012 | 22159754 |
| chemical composition and enzymatic digestibility of sugarcane clones selected for varied lignin content. | the recalcitrance of lignocellulosic materials is a major limitation for their conversion into fermentable sugars. lignin depletion in new cultivars or transgenic plants has been identified as a way to diminish this recalcitrance. in this study, we assessed the success of a sugarcane breeding program in selecting sugarcane plants with low lignin content, and report the chemical composition and agronomic characteristics of eleven experimental hybrids and two reference samples. the enzymatic diges ... | 2011 | 22145819 |
| chemical and morphological characterization of sugarcane bagasse submitted to a delignification process for enhanced enzymatic digestibility. | in recent years, biorefining of lignocellulosic biomass to produce multi-products such as ethanol and other biomaterials has become a dynamic research area. pretreatment technologies that fractionate sugarcane bagasse are essential for the successful use of this feedstock in ethanol production. in this paper, we investigate modifications in the morphology and chemical composition of sugarcane bagasse submitted to a two-step treatment, using diluted acid followed by a delignification process with ... | 2011 | 22122978 |
| genetically modified sugarcane for bioenergy generation. | sugarcane breeding has significantly progressed over the past 30 years, but attempts to further increase crop yield have been limited due to the complexity of the sugarcane genome. an alternative to boost the crop yield is the introduction of genes encoding desirable traits in the elite sugarcane cultivars. genetically modified sugarcane with increased yield and pest and disease resistance has already proven its value not only by the increased sugar content but also for the improvement of the cr ... | 2012 | 22093808 |
| integrated versus stand-alone second generation ethanol production from sugarcane bagasse and trash. | ethanol production from lignocellulosic materials is often conceived considering independent, stand-alone production plants; in the brazilian scenario, where part of the potential feedstock (sugarcane bagasse) for second generation ethanol production is already available at conventional first generation production plants, an integrated first and second generation production process seems to be the most obvious option. in this study stand-alone second generation ethanol production from surplus su ... | 2012 | 22019267 |
| sugarcane improvement: how far can we go? | in recent years, efforts to improve sugarcane have focused on the development of biotechnology for this crop. it has become clear that sugarcane lacks tools for the biotechnological route of improvement and that the initial efforts in sequencing ests had limited impact for breeding. until recently, the models used by breeders in statistical genetics approaches have been developed for diploid organisms, which are not ideal for a polyploid genome such as that of sugarcane. breeding programs are de ... | 2012 | 21983270 |
| screening and production of ligninolytic enzyme by a marine-derived fungal pestalotiopsis sp. j63. | marine-derived fungi are prone to produce structurally unique secondary metabolites, a considerable number of which display the promising biological properties and/or industrial applications. among those, ligninolytic enzymes have attracted great interest in recent years. in this work, about 20 strains were isolated from sea mud samples collected in the east china sea and then screened for their capacity to produce lignin-degrading enzymes. the results showed that a strain, named j63, had a grea ... | 2011 | 21947763 |
| evaluation of grinding methods for pellets preparation aiming at the analysis of plant materials by laser induced breakdown spectrometry. | it has been demonstrated that laser induced breakdown spectrometry (libs) can be used as an alternative method for the determination of macro (p, k, ca, mg) and micronutrients (b, fe, cu, mn, zn) in pellets of plant materials. however, information is required regarding the sample preparation for plant analysis by libs. in this work, methods involving cryogenic grinding and planetary ball milling were evaluated for leaves comminution before pellets preparation. the particle sizes were associated ... | 2011 | 21872013 |
| sugar cane burning pollution and respiratory symptoms in schoolchildren in monte aprazível, southeastern brazil. | to estimate the prevalence of respiratory symptoms and to analyze associated factors as well as peak expiratory flow measurements in schoolchildren. | 2011 | 21829978 |
| functional markers for gene mapping and genetic diversity studies in sugarcane. | the database of sugarcane expressed sequence tags (est) offers a great opportunity for developing molecular markers that are directly associated with important agronomic traits. the development of new est-ssr markers represents an important tool for genetic analysis. in sugarcane breeding programs, functional markers can be used to accelerate the process and select important agronomic traits, especially in the mapping of quantitative traits loci (qtl) and plant resistant pathogens or qualitative ... | 2011 | 21798036 |
| second generation ethanol in brazil: can it compete with electricity production? | much of the controversy surrounding second generation ethanol production arises from the assumed competition with first generation ethanol production; however, in brazil, where bioethanol is produced from sugarcane, sugarcane bagasse and trash will be used as feedstock for second generation ethanol production. thus, second generation ethanol production may be primarily in competition with electricity production from the lignocellulosic fraction of sugarcane. a preliminary technical and economic ... | 2011 | 21795041 |
| effects of soil mechanical resistance on nematode community structure under conventional sugarcane and remaining of atlantic forest. | nematodes present high potential as a biological indicator of soil quality. in this work, it was evaluated relations between soil physical properties and nematode community under sugarcane cropping and remaining of atlantic forest areas in northeastern pernambuco, brazil. soil samples were collected from september to november 2009 along two 200-m transects in both remaining of atlantic forest and sugarcane field at deeps of 0-10, 10-20, 20-30, 30-40, and 40-50 cm. for soil characterization, it w ... | 2012 | 21769561 |
| reuse of sugarcane bagasse ash (scba) to produce ceramic materials. | sugarcane bagasse ash (scba) is a residue resulting from the burning of bagasse in boilers in the sugarcane/alcohol industry. scba has a very high silica concentration and contains aluminum, iron, alkalis and alkaline earth oxides in smaller amounts. in this work, the properties of sintered ceramic bodies were evaluated based on the concentration of scba, which replaced non-plastic material. the ash was mixed (up to 60 wt%) with a clayed raw material that is used to produce roof tiles. prismatic ... | 2011 | 21733619 |
| induction of insect plant resistance to the spittlebug mahanarva fimbriolata stål (hemiptera: cercopidae) in sugarcane by silicon application. | changes in the agroecosystem with the increase of green cane harvesting in brazil affected the insect populations associated to this crop, and secondary pests like the spittlebug mahanarva fimbriolata stål, became much more important. many studies have demonstrated the active role played by silicon in plant defense against herbivory. the objective of this study was to evaluate the effects of silicon applications on the biology of the spittlebug reared on two resistant (sp79-1011 and sp80-1816) a ... | 2013 | 21710035 |
| cellulases and hemicellulases from endophytic acremonium species and its application on sugarcane bagasse hydrolysis. | the aim of this work was to have cellulase activity and hemicellulase activity screenings of endophyte acremonium species (acremonium zeae ea0802 and acremonium sp. ea0810). both fungi were cultivated in submerged culture (sc) containing l -arabinose, d -xylose, oat spelt xylan, sugarcane bagasse, or corn straw as carbon source. in solid-state fermentation, it was tested as carbon source sugarcane bagasse or corn straw. the highest fpase, endoglucanase, and xylanase activities were produced by a ... | 2011 | 21573756 |
| enzymatic hydrolysis of chemithermomechanically pretreated sugarcane bagasse and samples with reduced initial lignin content. | chemithermomechanical (ctm) processing was used to pretreat sugarcane bagasse with the aim of increasing cell wall accessibility to hydrolytic enzymes. yields of the pretreated samples were in the range of 75-94%. disk refining and alkaline-ctm and alkaline/sulfite-ctm pretreatments yielded pretreated materials with 21.7, 17.8, and 15.3% of lignin, respectively. hemicellulose content was also decreased to some extent. fibers of the pretreated materials presented some external fibrillation, fiber ... | 2011 | 21344677 |
| identification and expression analysis of micrornas and targets in the biofuel crop sugarcane. | micrornas (mirnas) are small regulatory rnas, some of which are conserved in diverse plant genomes. therefore, computational identification and further experimental validation of mirnas from non-model organisms is both feasible and instrumental for addressing mirna-based gene regulation and evolution. sugarcane (saccharum spp.) is an important biofuel crop with publicly available expressed sequence tag and genomic survey sequence databases, but little is known about mirnas and their targets in t ... | 2010 | 21092324 |
| characterization of commercial cellulases and their use in the saccharification of a sugarcane bagasse sample pretreated with dilute sulfuric acid. | this study aimed to correlate the efficiency of enzymatic hydrolysis of the cellulose contained in a sugarcane bagasse sample pretreated with dilute h(2)so(4) with the levels of independent variables such as initial content of solids and loadings of enzymes and surfactant (tween 20), for two cellulolytic commercial preparations. the preparations, designated cellulase i and cellulase ii, were characterized regarding the activities of total cellulases, endoglucanase, cellobiohydrolase, cellobiase, ... | 2011 | 20953894 |
| evaluating the use of predatory insects as bioindicators of metals contamination due to sugarcane cultivation in neotropical streams. | streams located in areas of sugarcane cultivation receive high concentrations of metal ions from soils of the adjacent areas causing accumulation of metals in the aquatic sediment. this impact results in environmental problems and leads to bioaccumulation of metal ions in aquatic organisms. in the present study, metal concentrations in different predatory insects were studied in streams near sugarcane cultivation and compared to reference sites. possible utilisation of predatory insects as bioin ... | 2011 | 20711857 |
| valorization of an industrial organosolv-sugarcane bagasse lignin: characterization and use as a matrix in biobased composites reinforced with sisal fibers. | in the present study, the main focus was the characterization and application of the by-product lignin isolated through an industrial organosolv acid hydrolysis process from sugarcane bagasse, aiming at the production of bioethanol. the sugarcane lignin was characterized and used to prepare phenolic-type resins. the analysis confirmed that the industrial sugarcane lignin is of hgs type, with a high proportion of the less substituted aromatic ring p-hydroxyphenyl units, which favors further react ... | 2010 | 20589841 |
| genetic variability of mahanarva sp (hemiptera: cercopidae) collected from different sites in brazil. | spittlebugs are the leading cause of damage to tall grasses. annual losses are estimated to reach 2.1 billion dollars in sugarcane crops and grazing land throughout the world. correct identification of these species is difficult due to similarities in color, body size and male genitalia. molecular markers have been useful in the identification and assessment of genetic diversity of many species. we investigated the genetic diversity of the spittlebug species mahanarva fimbriolata, m. spectabilis ... | 2010 | 20533195 |
| purification and biochemical properties of a glucose-stimulated beta-d-glucosidase produced by humicola grisea var. thermoidea grown on sugarcane bagasse. | the effect of several carbon sources on the production of mycelial-bound beta-glucosidase by humicola grisea var. thermoidea in submerged fermentation was investigated. maximum production occurred when cellulose was present in the culture medium, but higher specific activities were achieved with cellobiose or sugarcane bagasse. xylose or glucose (1%) in the reaction medium stimulated beta-glucosidase activity by about 2-fold in crude extracts from mycelia grown in sugarcane bagasse. the enzyme w ... | 2010 | 20221730 |
| use of brazilian sugarcane bagasse ash in concrete as sand replacement. | sugarcane today plays a major role in the worldwide economy, and brazil is the leading producer of sugar and alcohol, which are important international commodities. the production process generates bagasse as a waste, which is used as fuel to stoke boilers that produce steam for electricity cogeneration. the final product of this burning is residual sugarcane bagasse ash (sba), which is normally used as fertilizer in sugarcane plantations. ash stands out among agroindustrial wastes because it re ... | 2010 | 20163947 |
| heat causes oligomeric disassembly and increases the chaperone activity of small heat shock proteins from sugarcane. | small heat shock proteins (shsp) constitute an important chaperone family linked to conformational diseases. in plants, shsps prevent protein aggregation by acting as thermosensors and to enhance cell stress tolerance. sshsp17.2 and sshsp17.9 are the most highly expressed class i shsps in sugarcane. they exist as dodecamers at 20 degrees c and have distinct substrate specificities. therefore, they are useful models to study how class i shsps work. here we present data on the effects of heat on t ... | 2016 | 20137963 |
| xylooligosaccharides production from alkali-pretreated sugarcane bagasse using xylanases from thermoascus aurantiacus. | sugarcane bagasse hemicellulose was isolated in a one-step chemical extraction using hydrogen peroxide in alkaline media. the polysaccharide containing 80.9% xylose and small amounts of l-arabinose, 4-o-methyl-d-glucuronic acid and glucose, was hydrolyzed by crude enzymatic extracts from thermoascus aurantiacus at 50 degrees c. conditions of enzymatic hydrolysis leading to the best yields of xylose and xylooligosaccharides (dp 2-5) were investigated using substrate concentration in the range 0.5 ... | 2010 | 20066571 |
| removal of zn2+ from aqueous single metal solutions and electroplating wastewater with wood sawdust and sugarcane bagasse modified with edta dianhydride (edtad). | this work describes the preparation of a new chelating material derived from wood sawdust, manilkara sp., and not only the use of a new support, but also a chemically modified sugarcane bagasse synthesized in our previous work to remove zn(2+) from aqueous solutions and electroplating wastewater. the first part describes the chemical modification of wood sawdust and sugarcane bagasse using ethylenediaminetetraacetic dianhydride (edtad) as modifying agent in order to introduce carboxylic acid and ... | 2010 | 20047793 |
| efficient targeting of polyhydroxybutyrate biosynthetic enzymes to plant peroxisomes requires more than three amino acids in the carboxyl-terminal signal. | metabolic engineering of plant peroxisomes for biotechnological purposes typically requires efficient peroxisomal targeting of heterologous proteins. type i peroxisomal targeting signals (pts1) consist of three uncleaved amino acids (skl or a conserved variant) at the carboxyl terminus and direct nuclear-encoded proteins into the peroxisomes of eukaryotic cells. pts1 fusion with a heterologous protein results in peroxisomal targeting of that protein, but the minimal length of pts1 required for e ... | 2010 | 19879015 |
| scale-up of diluted sulfuric acid hydrolysis for producing sugarcane bagasse hemicellulosic hydrolysate (sbhh). | sugarcane bagasse was pretreated with diluted sulfuric acid to obtain sugarcane bagasse hemicellulosic hydrolysate (sbhh). experiments were conducted in laboratory and semi-pilot reactors to optimize the xylose recovery and to reduce the generation of sugar degradation products, as furfural and 5-hydroxymethylfurfural (hmf). the hydrolysis scale-up procedure was based on the h-factor, that combines temperature and residence time and employs the arrhenius equation to model the sulfuric acid conce ... | 2010 | 19846294 |
| sugarcane genes associated with sucrose content. | sucrose content is a highly desirable trait in sugarcane as the worldwide demand for cost-effective biofuels surges. sugarcane cultivars differ in their capacity to accumulate sucrose and breeding programs routinely perform crosses to identify genotypes able to produce more sucrose. sucrose content in the mature internodes reach around 20% of the culms dry weight. genotypes in the populations reflect their genetic program and may display contrasting growth, development, and physiology, all of wh ... | 2009 | 19302712 |
| monodisperse lignin fractions as standards in size-exclusion analysis: comparison with polystyrene standards. | the difficulty of preparing monodisperse lignin fractions on a large scale is a limiting factor in many applications. the present paper addresses this problem by examining the properties and size-exclusion behavior of lignin isolated by the acetosolv pulping process from post-extraction crushed sugarcane bagasse. the isolated lignin was subjected to a solvent pretreatment, followed by preparative gel permeation chromatography fractionation. the fractions were analyzed by high-performance size-ex ... | 2009 | 19269646 |
| biotechnological production of xylitol: enhancement of monosaccharide production by post-hydrolysis of dilute acid sugarcane hydrolysate. | dilute-acid hydrolysis pretreatment of sugarcane bagasse resulted in release of 48% (18.4 g/l) of the xylan in the hemicellulose fraction into the hydrolysate as monomeric xylose. in order to enhance the recuperation of this monomer, a post-hydrolysis stage consisted of thermal treatment was carried out. this treatment resulted in an increase in xylose release of 62% (23.5 g/l) of the hemicellulose fraction. original and post-hydrolysates were concentrated to the same levels of monomeric xylose ... | 2009 | 19214792 |
| characterization of a sugarcane (saccharum spp.) gene homolog to the brassinosteroid insensitive1-associated receptor kinase 1 that is associated to sugar content. | the present article reports on the characterization of scbak1, a leucine-rich repeat receptor-like kinase from sugarcane (saccharum spp.), expressed predominantly in bundle-sheath cells of the mature leaf and potentially involved in cellular signaling cascades mediated by high levels of sugar in this organ. in this report, it was shown that the scbak1 sequence was similar to the brassinosteroid insensitive1-associated receptor kinase1 (bak1). the putative cytoplasmatic domain of scbak1 contains ... | 2009 | 19096852 |
| informative genomic microsatellite markers for efficient genotyping applications in sugarcane. | genomic microsatellite markers are capable of revealing high degree of polymorphism. sugarcane (saccharum sp.), having a complex polyploid genome requires more number of such informative markers for various applications in genetics and breeding. with the objective of generating a large set of microsatellite markers designated as sugarcane enriched genomic microsatellite (segms), 6,318 clones from genomic libraries of two hybrid sugarcane cultivars enriched with 18 different microsatellite repeat ... | 2009 | 18946655 |
| biotechnological production of xylitol from agroindustrial residues. evaluation of bioprocesses. | batch, fed-batch, and semicontinuous fermentation processes were used for the production of xylitol from sugarcane bagasse hemicellulosic hydrolysate. the best results were achieved by the semicontinuous fermentation process: a xylitol yield of 0.79 g/g with an efficiency of 86% and a volumetric productivity of 0.66 g/l/h. | 1998 | 18576050 |
| carboxymethylcellulose obtained by ethanol/water organosolv process under acid conditions. | sugar cane bagasse pulps were obtained by ethanol/water organosolv process under acid and alkaline conditions. the best condition of acid pulping for the sugarcane bagasse was 0.02 mol/l sulfuric acid at 160 degrees c, for 1 h, whereas the best condition for alkaline pulping was 5% sodium hydroxide (base pulp) at 160 degrees c, for 3 h. for the residual lignin removal, the acid and alkaline pulps were submitted to a chemical bleaching using sodium chlorite. pulps under acid and alkaline conditio ... | 2007 | 18478417 |
| the brazilian biofuels industry. | ethanol is a biofuel that is used as a replacement for approximately 3% of the fossil-based gasoline consumed in the world today. most of this biofuel is produced from sugarcane in brazil and corn in the united states. we present here the rationale for the ethanol program in brazil, its present 'status' and its perspectives. the environmental benefits of the program, particularly the contribution of ethanol to reducing the emission of greenhouse gases, are discussed, as well as the limitations t ... | 2008 | 18471272 |
| oxidation in acidic medium of lignins from agricultural residues. | agricultural residues as sugarcane straw and bagasse are burned in boilers for generation of energy in sugar and alcohol industries. however, excess of those by-products could be used to obtain products with higher value. pulping process generates cellulosic pulps and lignin. the lignin could be oxidized and applied in effluent treatments for heavy metal removal. oxidized lignin presents very strong chelating properties. lignins from sugarcane straw and bagasse were obtained by ethanol-water pul ... | 2008 | 18418748 |
| influence of mahanarva fimbriolata (stål) (hemiptera: cercopidae) injury on the quality of cane juice. | mahanarva fimbriolata (stål) is an important pest in latin america and causes significant reduction in sugarcane productivity. there is no information regarding the effect of this pest on the quality of cane juice used for sugar and alcohol production. this work aimed at evaluating the quality of sugarcane juice from plants attacked by spittlebugs. the experiment was arranged in a completely randomized design with 15 replications, and comprised two treatments: control and chemical treatment with ... | 2013 | 18368252 |
| [sugar cane burning in brazil: respiratory health effects]. | the article aimed to update scientific literature information about respiratory health effects caused by sugarcane burning, considering the expansion of sugarcane plantations in brazil and in the state of são paulo. articles published between 1996 and 2006, which deal with the health effects of sugarcane burning and/or air pollutants originating from this burning, were discussed. these studies suggest that part of the population--especially the elderly, children and asthmatics--suffers health ef ... | 2008 | 18327496 |
| sugarcane functional genomics: gene discovery for agronomic trait development. | sugarcane is a highly productive crop used for centuries as the main source of sugar and recently to produce ethanol, a renewable bio-fuel energy source. there is increased interest in this crop due to the impending need to decrease fossil fuel usage. sugarcane has a highly polyploid genome. expressed sequence tag (est) sequencing has significantly contributed to gene discovery and expression studies used to associate function with sugarcane genes. a significant amount of data exists on regulato ... | 2008 | 18273390 |
| identification of new aba- and meja-activated sugarcane bzip genes by data mining in the sucest database. | sugarcane is generally propagated by cuttings of the stalk containing one or more lateral buds, which will develop into a new plant. the transition from the dormant into the active stage constitutes a complex phenomenon characterized by changes in accumulation of phytohormones and several other physiological aspects. abscisic acid (aba) and methyl-jasmonate (meja) are major signaling molecules, which influence plant development and stress responses. these plant regulators modulate gene expressio ... | 2008 | 17968554 |
| leaching and half-life of the herbicide tebuthiuron on a recharge area of guarany aquifer in sugarcane fields in brazil. | this study was undertaken to evaluate the degradation and mobility of the herbicide tebuthiuron (n-[5-(1,1-dimethylethyl)-1,3,4-thiadiazol-2-yl]-n,n'-dimethylurea) in soil under field conditions, and its potential for leaching and groundwater contamination. a watershed, espraiado, located over a recharge area in brazil, was chosen for soil and water studies. at espraiado, water samples were collected from seven wells at intervals of three months from march 2004 to june 2006 and analyzed for tebu ... | 2007 | 17701698 |
| identification of genes responsive to the application of ethanol on sugarcane leaves. | the control of gene expression in precise time and space is a desirable attribute of chemically inducible systems. ethanol is a chemical inducer with favourable features, such as being inexpensive and easy to apply. the aim of this study was to identify ethanol-responsive genes in sugarcane. the cdna macroarray technique was adopted to identify transcript changes in sugarcane leaves (saccharum spp. cv sp80-3280) exposed to ethanol. the expression profiles of sugarcane genes were analysed using n ... | 2007 | 17701412 |
| pipeline for macro- and microarray analyses. | the pipeline for macro- and microarray analyses (pmma) is a set of scripts with a web interface developed to analyze dna array data generated by array image quantification software. pmma is designed for use with single- or double-color array data and to work as a pipeline in five classes (data format, normalization, data analysis, clustering, and array maps). it can also be used as a plugin in the bioarray software environment, an open-source database for array analysis, or used in a local versi ... | 2007 | 17464422 |
| orthologous comparison in a gene-rich region among grasses reveals stability in the sugarcane polyploid genome. | modern sugarcane (saccharum spp.) is an important grass that contributes 60% of the raw sugar produced worldwide and has a high biofuel production potential. it was created about a century ago through hybridization of two highly polyploid species, namely s. officinarum and s. spontaneum. we investigated genome dynamics in this highly polyploid context by analyzing two homoeologous sequences (97 and 126 kb) in a region that has already been studied in several cereals. our findings indicate that t ... | 2007 | 17425713 |
| biochemical and biophysical characterization of small heat shock proteins from sugarcane. involvement of a specific region located at the n-terminus with substrate specificity. | when cells are submitted to an increase in temperature, heat shock proteins (hsp) are synthesized to help heat stress resistance. small hsps, which are diverse and abundant in plants, have the major function of preventing irreversible protein aggregation. the diversity of small hsps in plants is intriguing and characterization of their chaperone activity is important to understand plant tolerance to heat stress. a previous study showed that small hsps, mainly represented by class i (cytosolic), ... | 2007 | 17336576 |
| mudra-like sequences from rice and sugarcane cluster as two bona fide transposon clades and two domesticated transposases. | the mutator system of maize has been described as the most active and mutagenic plant transposon. the autonomous element mudr contains two genes: mudra encoding the transposase and mudrb whose product function remains undetermined. mudra-like coding domain showed to be the most abundantly expressed transposon-related sequence in sugarcane transcriptome. a previous report identified the existence of at least four clades of mudra-like sequences in sugarcane, rice and arabidopsis, which already exi ... | 2007 | 17289300 |
| serial analysis of gene expression in sugarcane (saccharum spp.) leaves revealed alternative c4 metabolism and putative antisense transcripts. | sugarcane (saccharum spp.) is a highly efficient biomass and sugar producing crop. leaf reactions have been considered as potential rate-limiting step for sucrose accumulation in sugarcane stalks. to characterize the sugarcane leaf transcriptome, field-grown mature leaves from cultivar "sp80-3280" were analyzed using serial analysis of gene expression (sage). from 480 sequenced clones, 9,482 valid tags were extracted, with 5,227 unique sequences, from which 3,659 (70%) matched at least a sugarca ... | 2007 | 17211512 |
| response of sugarcane to increasing concentrations of copper and cadmium and expression of metallothionein genes. | sugarcane (saccharum spp.) offers the potential to be a phytoremediator species due to its outstanding biomass production, but its prospective metal accumulation and tolerance have not been fully characterized. sugarcane plantlets were able to tolerate up to 100microm of copper in nutrient solution for 33 days, with no significant reduction in fresh weight, while accumulating 45mgcukg(-1) shoot dry weight. higher levels of copper in solution (250 and 500microm) were lethal. sugarcane displayed t ... | 2007 | 17175063 |
| [screening of microorganism producing polyunsaturated fatty acids with sugarcane molasses]. | a strain name lb1 producing polyunsaturated fatty acids with sugarcane molasses was screened form soil samples by the methods of sudan black b stain and the determination of pufas' iodine value. based on the grass morphological and physiological characteristics and the sequence similarity of ribosomal dna-its, the strain was identified as mucor recurvus sp.. the results of l64 (4(3)) orthogonal experiments indicated when mucor recurvus was cultivated at 28 degrees c for 5 days with 160 r/min, th ... | 2006 | 17037053 |
| female reproductive system of the sugarcane spittlebug mahanarva fimbriolata (auchenorrhyncha): vitellogenesis dynamics and protein quantification. | the present study aimed describing the ovaries of the sugarcane spittlebug mahanarva fimbriolata which are meroistic telotrophic with nurse cells and oocytes located in the tropharium. sem revealed paired ovaries located dorsolaterally around the intestine, and oocytes exhibiting shapes ranging from round (less developed) to elliptic (more developed), suggesting a simultaneous, although, asynchronous development. based on histological data we classified the oocytes in stages from i to v. stage i ... | 2007 | 16973368 |
| effects of genetic polymorphisms cyp1a1, gstm1, gstt1 and gstp1 on urinary 1-hydroxypyrene levels in sugarcane workers. | sugarcane workers in brazil are exposed to various genotoxic compounds, including polycyclic aromatic hydrocarbons (pahs), derived from an incomplete combustion process of burnt sugarcane fields. the effects of the occupational exposure to sugarcane fields burning were measured in urine samples of sugarcane workers from the northwest of the state of são paulo when exposed (harvesting) and when non-exposed (non-harvesting). the urinary levels of 1-hydroxypyrene (1-ohp) and the influence of the ge ... | 2006 | 16914185 |
| ultrastructural study of the ovary of the sugarcane spittlebug mahanarva fimbriolata (hemiptera). | the present study describes the ultrastructure of meroistic telotrophic ovaries of the sugarcane spittlebug mahanarva fimbriolata. in this type of ovary, nurse cells, oogonia, and prefollicular tissue are located at the terminal (distal) regions or tropharium of ovarioles. oocytes in different developmental stages, classified from i to v, are observed in the vitellarium. stage i oocytes do not exhibit intercellular spaces in the follicular epithelium, suggesting that synthesis and production of ... | 2006 | 16644228 |
| comparison of stir bar sorptive extraction and membrane-assisted solvent extraction as enrichment techniques for the determination of pesticide and benzo[a]pyrene residues in brazilian sugarcane juice. | the present work compares two modern and environmentally friendly enrichment techniques, stir bar sorptive extraction (sbse) and membrane-assisted solvent extraction (mase) for the determination of 18 organic contaminant residues in brazilian sugarcane juice. stir bar sorptive extraction and thermal desorption coupled to capillary gas chromatography-mass spectrometry using the selected ion monitoring mode [sbse-td-gc-ms(sim)] and membrane-assisted solvent extraction combined with large volume in ... | 2006 | 16597442 |
| variables that affect xylitol production from sugarcane bagasse hydrolysate in a zeolite fluidized bed reactor. | the operational conditions for xylitol production by fermentation of sugarcane bagasse hydrolysate in a fluidized bed reactor with cells immobilized on zeolite were evaluated. fermentations were carried out under different conditions of air flowrate (0.0125-0.0375 vvm), zeolite mass (100-200 g), initial ph (4-6), and xylose concentration (40-60 g/l), according to a 2(4) full factorial design. the air flowrate increase resulted in a metabolic deviation from product to biomass formation. on the ot ... | 2013 | 16321046 |
| determination of flavonoids in cultivated sugarcane leaves, bagasse, juice and in transgenic sugarcane by liquid chromatography-uv detection. | a high-performance liquid chromatography (hplc) method with photo-diode array (dad) detection was developed to separate and quantify flavonoids in sugarcane leaves and bagasse (= the crushed sugarcane refuse from juice extraction), and in sugarcane juice. sugarcane flavonoids consist of a complex mixture of aglycones and glycosides (including flavonolignan glycosides), and the hplc-uv method herein proposed is suitable for their quantification as total flavonoids. this method was applied to anal ... | 2006 | 16310199 |
| development of an integrated genetic map of a sugarcane (saccharum spp.) commercial cross, based on a maximum-likelihood approach for estimation of linkage and linkage phases. | sugarcane (saccharum spp.) is a clonally propagated outcrossing polyploid crop of great importance in tropical agriculture. up to now, all sugarcane genetic maps had been developed using either full-sib progenies derived from interspecific crosses or from selfing, both approaches not directly adopted in conventional breeding. we have developed a single integrated genetic map using a population derived from a cross between two pre-commercial cultivars ('sp80-180' x 'sp80-4966') using a novel appr ... | 2006 | 16307229 |
| effects of sulfuric acid loading and residence time on the composition of sugarcane bagasse hydrolysate and its use as a source of xylose for xylitol bioproduction. | a 2(2) full factorial design was employed to evaluate the effects of sulfuric acid loading and residence time on the composition of sugarcane bagasse hydrolysate obtained in a 250-l reactor. the acid loading and the residence time were varied from 70 to 130 mg acid per gram of dry bagasse and from 10 to 30 min, respectively, while the temperature (121 degrees c) and the bagasse loading (10%) were kept constant. both the sulfuric acid loading and the residence time influenced the concentrations o ... | 2013 | 16209549 |
| ultrastructural study of the salivary glands of the sugarcane spittlebug mahanarva fimbriolata (stal, 1854) (euhemiptera: cercopidae). | spittlebugs are insects that suck sap from plants and regurgitate saliva containing toxic enzymes into the leaves. as a consequence, the conductive channels are blocked resulting in dry leaves, thus giving a burned aspect to the plantation. this work performed ultrastructural analyses of the salivary glands of the sugarcane spittlebug mahanarva fimbriolata, since these organs produce the enzymes that are injected into the plants, thus being responsible for the economic losses in the production o ... | 2006 | 16171997 |
| on-line identification of sugarcane (saccharum officinarum l.) methoxyflavones by liquid chromatography-uv detection using post-column derivatization and liquid chromatography-mass spectrometry. | sugarcane (saccharum officinarum l., gramineae) bagasse and leaves were investigated for their flavonoid content and transgenic sugarcane ("bowman-birk" and "kunitz") was compared with non-modified ("control") plants. analyses were carried out by high-performance liquid chromatography coupled to diode array uv detection (lc/uv), also using post-column addition of shift reagents, and tandem ms (atmospheric pressure chemical ionization-ms/ms and collision-induced dissociation-ms). on-line uv and m ... | 2005 | 16038194 |
| ceriporiopsis subvermispora used in delignification of sugarcane bagasse prior to soda/anthraquinone pulping. | sugarcane bagasse was pretreated with the white-rot fungus ceriporiopsis subvermispora for 30 d of incubation. the solid-state fermentation of 800 g of bagasse was carried out in 20-l bioreactors with an inoculum charge of 250 mg of fungal mycelium/kg of bagasse. the oxidative enzymes manganese peroxidase (mnp), lignin peroxidase (lip), and laccase (lac) and the hydrolytic enzyme xylanase (xyl) were measured by standard methods and related to the fungus's potential for delignification. among the ... | 2005 | 15920273 |
| perspectives on bioenergy and biotechnology in brazil. | brazil is one of the world's largest producers of alcohol from biomass at low cost and is responsible for more than 1 million direct jobs. in 1973, the brazilian program of alcohol (proalcool) stimulated the creation of a bioethanol industry that has led to large economic, social, and scientific improvements. in the year 1984, 94.5% of brazil's cars used bioethanol as fuel. in 2003/2004, 350.3 million of sugarcane produced 24.2 million t of sugar and 14.4 billion l of ethanol for an average 4.3 ... | 2005 | 15917587 |