| [relations between catabolite repression and sporulation in bacillus subtilis (author's transl)]. | acetoin dehydrogenase can be catabolite repressed by numerous sources of carbon. the following results point out that the catabolite repression of this enzyme and the inhibition of sporulation are mediated by the same mechanism: 1. mutants, able to synthesize acetoin dehydrogenase in the presence of glucose, sporulate in glucose medium at a higher rate than the standard strain. 2. the catabolite repressing effect of a compound and its ability to inhibit sporulation are in a direct relation to ea ... | 1976 | 822795 |
| electron microscopy of isolated cell walls of bacillus subtilis var. niger. | isolated cell walls of bacillus subtilis have astriated appearance in the electron microscope. the structure persists when teichoic acids are removed. it is inferred that the structure bears on the arrangement of the peptidoglycan chains. | 1976 | 822796 |
| some physiological functions of the l-leucine dehydrogenase in bacillus subtilis. | mutants of bacillus subtilis constitutive for l-leucine dehydrogenase synthesis were selected. using these mutants we could determine two functional roles for the l-leucine dehydrogenase. this enzyme liberates ammonium ions from branched chain amino acids when supplied as the sole nitrogen source. another function is to synthesize from l-isoleucine, l-leucine, and l-valine the branched chain alpha-keto acids which are precursors of branched chain fatty acid biosythesis. these results together wi ... | 1976 | 822797 |
| vinyl chloride mutagenicity via the metabolites chlorooxirane and chloroacetaldehyde monomer hydrate. | mutagenicity tester strains of bacillus and salmonella were used to assay vinyl chloride in nutrient broth at a practical concentration level. also screened without exogenous activation were seven potential metabolites of vinyl chloride in their pure forms as well as the related epichlorohydrin. chlorooxirane, chloroacetaldehyde, chloroacetaldehyde monomer hydrate, chloroacetaldehyde dimer hydrate, chloroacetaldehyde trimer, and epichlorohydrin produced significant mutagenic acitivity in salmone ... | 1976 | 822875 |
| reconstitution of reduced nicotinamide adenine dinucleotide oxidase activity with menadione in membrane vesicles from the menaquinone-deficient bacillus subtilis aro d. relation between electron transfer and active transport. | membrane vesicles from the menaquinone-deficient bacillus subtilis aro d contain a low content of menaquinone and consequently oxidaze reduced nicotinamide adenine dinucleotide (nadh) at low rate. supplementation of the membrane vesicles suspension with the menaquinone-analogue menadione, results in an incorporation of menadione in the membranes. the incorporated menadione increases with the external menadione concentration up to a maximum of 7 nmol of menadione bound per mg membrane protein. th ... | 1976 | 823014 |
| alteration of the acidic ribosomal proteins from dormant spores of bacillus subtilis. | | 1976 | 823046 |
| influence of detergent on aerosol allergic sensitization with enzymes of bacillus subtilis. | an anionic detergent, sodiumdodecylbenzenesulphonate, had an adjuvant effect upon aerosol allergic sensitization with subtilopeptidase a, a proteolytic enzyme of bacillus subtilis. both local and systemic antibody responses were accelerated and prolonged by use of the detergent. this adjuvant effect was only seen when an inactive form of the enzyme was used. the detergent, however, was able to potentiate early clinical responses upon initial exposure to the active enzyme. | 1976 | 823120 |
| evaluation of two microbiological methods for detecting residual antibiotics in milk. | two methods described in the aoac official methods of analysis for the detection of penicillin residues in whole milk were evaluated to determine the capability of each method to detect residues of 12 antibiotics used in the dairy industry. the first method, a cylinder-plate method that uses sarcina lutea as the test organism, detected levels of 1 ppm of 8 of the 12 antibiotics tested. the second method, using paper disks with bacillus subtilis as the test organism, detected approximately 1 ppm ... | 1976 | 823145 |
| structure of plant cell walls. purification and characterization of a beta-1,4-galactanase which degrades a structural component of the primary cell walls of dicots. | wild type bacillus subtilis, when grown on a soybean arabinan-galactan, secretes a beta-1,4-galactanase which has been purified more than 200-fold from the culture fluid. affinity chromatography was the most effective step in a purification procedure which resulted in a preparation that contained only a single 40,000 molecular weight protein band upon sodium dodecyl sulfate-disc gel electrophoresis. the purified galactanase digests a beta-1,4-galactan purified from citrus pectin and digests part ... | 1976 | 823153 |
| identification of an isolated protein essential for peptidoglycan synthesis as the n-acetylglucosaminyltransferase. | we have identified a protein extractable from bacillus megaterium membranes by licl as the enzyme in peptidoglycan synthesis which catalyzes the transfer of n-acetylglucosamine from udp-n-acetylglucosamine to n-acetyl-d-muramyl-l-analyl-d-gamma-glutamyl-meso-diaminopimelyl-d-alanyl-d-alanine-p-p-lipid. this identification demonstrates the isolation and characterization of a membrane-associated enzyme involved in a complex series of reactions without the ready availability of a substrate for the ... | 1976 | 823160 |
| sequence characterization of 5s ribosomal rna from eight gram positive procaryotes. | the available comparative data on procaryotic 5s rrna was extended through sequencing studies of eight gram positive procaryotes. complete nucleotide sequences were presented for 5s rrna from bacillus subtilis, b. firmus, b. pasteurii, b. brevis, lactobacillus brevis and streptococcus faecalis. in addition, 5s rrna oligonucleotide catalogs and partial sequence data were provided for b. cereus and sporosarcina ureae. these sequences and catalogs were discussed in terms of known features of procar ... | 1976 | 823342 |
| rec assay with cold incubation with and without metabolic reactivation in vitro [proceedings]. | | 1976 | 823428 |
| [dose-response relationship between the number of microorganisms and their radiation resistance]. | | 1976 | 823596 |
| [heterogeneity of rna polymerase in bacillus subtilis (author's transl)]. | | 1976 | 823604 |
| [in vitro synthesis of ribosomal rna (author's transl)]. | | 1976 | 823605 |
| [antibiotic residues in organs and muscle tissues of broilers. i. bacitracin, flavomycin, spiramycin and viriniamycin residues following administration of diets containing low levels of these antibiotics (author's transl)]. | two groups of broilers were fed two different feed mixtures. a feed containing a mixture of bacitracin, flavomycin, spiramycin and virginiamycin (20 ppm each) was administered to sixity broilers. sixty other broilers were given a similar feed not containing any antibiotics. after slaughter, samples of kidney, liver and breast were examined for the presence of antibiotic residues. all samples were found to be negative for antibiotic residues. four micro-organisms were used in performing the tests ... | 1976 | 823664 |
| [excretion of organic compounds bei microorganisms in presence of diphenylamine]. | diphenylamine (dpa) causes an increased leakage of organic compounds with resting cells of candida guilliermondii, bacillus subtilis, escherichia coli, and staphylococcus aureus already after a treatment of 1 h. the leakage depends both on the concentration of dpa and the duration of the treatment. moreover, dpa influences the composition of the leaked ninhydrine-positive compounds. after 20 h of treatment an increased release of mannan and ribose is observed in addition to the stronger leakage ... | 1976 | 823713 |
| [comparative studies on the effectiveness of 2 frequently-used elective culture media for enterococci]. | | 1976 | 823722 |
| [hygienic and bacteriological comparative studies in 50 hospitals. i. methods and aim of microbiological monitoring (author's transl)]. | within a period of a year, environmental examinations were carried out in 50 hospitals selected at random in rheinland-pfalz with a view to determining the effective conditions, mainly in such risky areas as operating theatres, delivery rooms, intensive-care units and neonatal wards. in this first report the method is described. such investigations are based on a detailed interrogation of the staff, and local inspection. as is evident from the extract from the questionnaire, the investigation wa ... | 1976 | 823739 |
| effect of ultrasonic waves on the heat resistance of bacillus spores. | the effect of ultrasonic treatment on the heat resistance of bacillus spores differs widely both with the species and the strain tested. ultrasonic waves do not affect the heat resistance of some strains, whereas they greatly reduce that of other strains of the same species. the heat-sensitizing effect of ultrasonic waves is more pronounced when short heating periods are used. ultrasonic treatment does not seem to affect the "z value". | 1976 | 823864 |
| effects of thermoradiation on bacteria. | a 60co source was used to determine the effects of thermoradiation on achromobacter aquamarinus, staphylococcus aureus, and vegetative and spore cells of bacillus subtilis var. globigii. the rate of inactivation of these cultures, except vegetative-cell populations of b. subtilis, was exponential and in direct proportion to temperature. the d10 (dose that inactivates 90% of the microbial population) value for a. aquamarinus was 8.0 krad at 25 degrees c and 4.9 krad at 35 degrees c. for s. aureus ... | 1976 | 823869 |
| s-adenosylmethionine and tetrahydrofolate-dependent methylation of trna in bacillus subtilis. incomplete methylations caused by trimethoprim, pactamycin, or chloramphenicol. | | 1976 | 823871 |
| [the effect of microorganisms on protein catabolism in putrefaction studies]. | | 1976 | 823935 |
| superactivation of neutral proteases: acylation with n-hydroxysuccinimide esters. | a series n-hydroxysuccinimide esters of acylamino acids previously shown to acylate and thereby increase the activity of thermolysin by several orders of magnitude (blumberg, s., and vallee, b. l. (1975), biochemistry 14, 2410) has been used to modify the related neutral proteases from bacillus subtilis, bacillus megaterium, and aeromonas proteolytica. each of these enzymes is activated to a level characteristic of the particular protein and the particular acyl group incorpporated when monitored ... | 1976 | 823965 |
| [effect of endonuclease isolated from sheep brains on dna--rna hybrids]. | | 1976 | 823981 |
| [use of eco ri restrictase for identifying closely related microorganisms]. | | 1976 | 824112 |
| [preparation of molecules combining phage lambda and bacillus subtilis dnas by the sticky end addition method]. | | 1976 | 824113 |
| pleiotropic, extragenic suppression of dna mutants in bacillus subtilis. | thermosensitive (dna) mutants of bacillus subtilis defective in deoxyribonucleic acid replication can be divided into two groups on the basis of their ability to spontaneously yield secondary mutants with an hds phenotype (thermoin-sensitivity and resistance to aryl-azo-pyrimidines) at frequencies higher than 10(-8). such a phenotype is due to alleles at the hds locus (mapping close to cysa), which act as extragenic pleiotropic suppressors. hds suppressibility has been used as a screening tool t ... | 1976 | 824267 |
| regulation of histidinol phosphate aminotransferase synthesis by tryptophan in bacillus subtilis. | the effect of tryptophan on the synthesis of histidinol phosphate aminotransferase and prephenate dehydrogenase has been examined. the genes specifying two enzymes for tryptophan biosynthesis (anthranilate synthase and tryptophan synthase-b) were found to be derepressed in a temporal sequence according to their chromosomal location. the genes for histidinol phosphate aminotransferase and prephenate dehydrogenase were derepressed simultaneously approximately 8 min after tryptophan synthase-b. whe ... | 1976 | 824269 |
| changes in deoxyribonucleic acid polymerase activities in synthesis of deoxyribonucleic acid during sporulation of bacillus subtilis. | the deoxyribonucleic acid (dna) polymerase activities in bacillus subtilis strains marburg 168 (thy-trp2) and d22, a dna polymerase i-deficient mutant, were measured at various stages of sporulation. the dna polymerase i activity, which had decreased after the exponential growth, began to increase at the early stage of sporulation, reached a maximum and then again decreased. the activity of neither dna polymerase ii nor iii was observed to change so drastically as that of dna polymerase i during ... | 1976 | 824270 |
| postirradiation recovery dependent on the uvr-1 locus in bacillus subtilis. | a mutant (uvr-1) of bacillus subtilis that is deficient in excision of ultraviolet (uv)-induced pyrimidine dimers from deoxyribonucleic acid (dna) shows a marked increase in ability to survive uv irradiation when plated on amino acid-supplemented agar medium compared with its survival ability when plated on nutrient plating medium, the effect is considered to be one of growth-dependent lethality. irradiated stationary phase uvr-1 cells, incubated in liquid medium lacking amino acids required for ... | 1976 | 824273 |
| site-specific deoxyribonucleases in bacillus subtilis and other bacillus strains. | we systematically studied site-specific deoxyribonucleases in bacillus strains and detected deoxyribonuclease activities in 20 of 62 strains tested. | 1976 | 824277 |
| resporulation of outgrowing bacillus subtilis spores. | germinated spores of bacillus subtilis were incubated in outgrowth medium and tested periodically for capacity to sporulate when suspended in sporulation medium. concurrent measurements were made of deoxyribonucleic acid (dna) content and numbers of cell division septa and nucleoids. sporulation potential is shown to reach a peak at about 110 min at which time the chromosomes are probably well into the second round of replication. experiments with nalidixic acid show that sporulation potential c ... | 1976 | 824279 |
| effect of netropsin on the derepression of enzymes during growth and sporulation of bacillus subtilis. | netropsin, a polypeptide antibiotic which binds specifically to adenylate-thymidylate-rich regions of deoxyribonucleic acid, inhibitis sporulation at about stage ii, but does not inhibit growth of bacillys subtilis. an analysis of the sporulation-associated enzymes aconitase, alkaline phosphatase, and glucose dehydrogenase revealed that their rates of expression were not affected by the presence of the antibiotic. the derepression of histidase, a vegetatively induced enzyme was stimulated by net ... | 1976 | 824280 |
| dna synthesis in vivo in bacillus subtilis. | | 1976 | 824451 |
| restriction and modification in bacillus subtilis: inducibility of a dna methylating activity in nonmodifying cells. | the nonrestricting/nonmodifying strain bacillus subtilis 222 (r-m-) can be induced to synthesize a dna-modifying activity upon treatment with either mitomycin c (mc) or uv light. this is shown by the following facts. (i) infection of mc-pretreated 222 cells with unmodified spp1 phage yields about 3% modified phage that are resistant to restriction in b. subtilis r (r+m+). the induced modifying activity causes the production of a small fraction of fully modified phage in a minority class of mc-tr ... | 1976 | 824459 |
| prophage mutation causing heat inducibility of defective bacillus subtilis bacteriophage pbsx. | a mutant of bacillus subtilis 168 has been isolated in which the defective phage pbsx was heat inducible, whereas another phage, phi105, was not so induced. a culture of the mutant grown at 30 degrees c, when shifted to 45 degrees c, began to lyse after 45 min; cell viability began to decrease after 10 min. heat-induced lysis of the mutant was prevented by chloramphenicol. dna, rna, protein, and peptidoglycan synthesis were normal at the nonpermissive temperature up to the time of lysis. the sit ... | 1976 | 824461 |
| genetic evidence that bacillus bacteriophage phi 105 integrates by insertion. | genetic analysis by pbs1 transduction has demonstrated that bacterial markers spanning the phi105 attachment site are spread upon integration, accounting for insertion of one or more phi105 genomes. | 1976 | 824462 |
| bacteriophage pbs2-induced inhibition of uracil-containing dna degradation. | degradation of uracil-containing dna by bacillus subtilis extracts and its inhibition after infection by the uracil-containing dna phage pbs2 have been investigated to resolve differences between the published reports of tomita and takahashi (1975) and friedberg et al. (1975, 1976). the product of hydrolysis of pbs2 dna, tritium labeled in its uracil and cytosine residues, is solely uracil and not deoxyuridine. the degrading activity is completely inhibited within 7 min after pbs2 infection, bef ... | 1976 | 824463 |
| [effect of ca2+ ions on late stages of genetic transformation in bacillus subtilis]. | calcium chloride stimulated later stages of genetic transformation after irreversible binding of dna in the cells of bacillus subtilis. the composition of a solid medium with cacl2 for selection after transformation is presented. | 1976 | 824529 |
| [use of hydropyrite in the chemiluminescent determination of iron-porphyrin proteins]. | hydropyrite was found to be a suitable reagent which may be used in chemiluminescent reactions for determination of iron-porphyrin proteins. | 1976 | 824530 |
| synthesis of rna and protein in a mutant of bacillus subtilis temperature sensitive during spore germination. | bacillus subtilis strain pb 2427 temperature sensitive in the synthesis of rna during spore germination and outgrowth has been characterized to some extent. at non permissive temperature (46 degrees c) strain pb 2427 synthesizes stable and unstable rna for 50 min from the beginning of germination and then stops. most of the stable rna is degraded to shorter molecules but can be identified as ribosomal rna by hybridization-competition experiments. at non permissive temperature, in the presence of ... | 1976 | 824546 |
| near-uv effects of 5,7-dimethoxycourmain in bacillus subtilis. | | 1976 | 824658 |
| [microbiological assay of some phenothiazine derivatives using different strains of bacillus subtilis]. | this paper deals with the microbiological assay of chloropromazine, levomepromazine and promethazine, whose bacteriostatic power was measured in the diffusion test against four different strains of bacillus subtilis. the four strains proved to be equally sensitive to solutions of phenothiazine derivatives containing more than 0.5 mg/ml. there was no difference between the four strains as referred to the median percentual error in the chlorpromazine determination. the median percentual error has ... | 1976 | 824694 |
| physical map of bacteriophage phi29 dna. | | 1976 | 824814 |
| [beta-1,3-1,4-glucanase in spore-forming microorganisms. i. beta-glucanase production during the growth cycle of bacillus subtilis (marburg yale)]. | | 1976 | 824871 |
| effect of nisin on the germination and proliferation of spores of some aerobic spore-formers in nutrient medium. | | 1976 | 824886 |
| effect of nisin in the presence of l-alanine on the germination and outgrowth of b. sublitis spores. | | 1976 | 824887 |
| effect of nisin on prolonging the keeping quality of pasteurized milk. | | 1976 | 824888 |
| [pathogenic properties of bacteria of the subtilis--mesentericus group]. | | 1976 | 824920 |
| glycerol dissimilation and its regulation in bacteria. | | 1976 | 825019 |
| active metabolites of isoxazolylpencillins in humans. | metabolites of the isoxazolylpenicillins that still possessed antibacterial activity were shown to be present in urine and serum. in healthy subjects, the amounts excreted in urine were low; 10 to 23% of the excreted penicillin activities represented the metabolites. the highest amount of metabolite in urine was found for oxacillin, and the lowest was found for flucloxacillin. no extreme differences in the amounts of metabolite excreted were observed when the compounds were administered orally o ... | 1976 | 825029 |
| microbiological hazard from the exhaust of a high-vacuum sterilizer. | data are presented which show the potential for release of viable microorganisms into the atmosphere from high-vacuum steam sterilizers during the evacuation cycle preceding application of steam under pressure. bacillus subtilis var. niger spores, serratia marcescens cells, and t1 coliphage disseminated into the sterilizer chamber as small particles from liquid suspensions, and dried spores of b. subtilis var. niger distributed on bulk discard materials were recovered from the atmosphere around ... | 1976 | 825044 |
| [mode of action of d-amino acids on the biosynthesis of peptidoglycan (author's transl)]. | the mechanism of growth inhibition by d-amino acids was studied. d-serine at concentrations from 0.02-0.2 m was sufficient to cause partial growth inhibition in seven species of bacteria representing the four most common types of peptidoglycan. the inhibited cells displayed morphological alterations. in the nucleotide-activated peptidoglycan precursors of these cells, d-alanine residues in position 4 and/or 5 of the peptide moiety were partially or even completely replaced by d-serine. the pepti ... | 1976 | 825075 |
| surface analysis and depth profile composition of bacterial cells by x-ray photoelectron spectroscopy and oxygen plasma etching. | | 1976 | 825123 |
| reversibility of partial denaturation of dna. | the hypochromicity recovery of a partially denatured dna sample when salt concentration is suddenly increased at an intermediate stage of the transition, is studied. the results of csc1 gradient analysis, polyethylenglycol/dextran partition analysis, and the behaviour in a new thermal transition, support the view that the process is an intramolecular double chain renaturation as that induced by cooling. the degree of denaturation irreversibility is dependent on the size of dna segments between t ... | 1976 | 825148 |
| immobilization of protein and polysaccharide on magnetic particles: selective binding of microorganisms by concanavalin a-magnetite. | | 1976 | 825154 |
| [effect of contamination of germ-free guinea pigs by individual representatives of the intestinal microflora on antibody and complement concentrations]. | on the 3rd day after birth germ-free guinea pigs were contaminated with one of the following representatives of normal intestinal microbial flora: bac. mesentericus, bac. subtilis, s. albus, and s. faecalis. in the animals aged two weeks a study was made of the level of antibodies against the microorganisms used for monocontamination, and also to the e. coli o55 pathogenic for guinea pigs; the complement content in the serum was examined as well. bac. mesentericus and bac. subtilis contamination ... | 1976 | 825158 |
| biosynthesis of the unit that links teichoic acid to the bacterial wall: inhibition by tunicamycin. | | 1976 | 825388 |
| 5-methoxyuridine, a new modified constituent in trnas of bacillaceae. | | 1976 | 825391 |
| [temperature-dependent mutants of bacillus subtilis a-50 with a diminished level of alkaline proteinase synthesis]. | temperature-sensitive proteolytically inactive mutants of bacillus subtilis a-50 are obtained. the optimal ability to synthetise the enzyme both in the original strain and in mutants is expressed at 37 degrees c. the level of proteolytic activity of selected prot(ts) mutants depends both on the temperature of growth and on the temperature at which the activity of subtilisin is analysed. the optima of functioning ts-proteinases are shifted in mutants as compared with the original strain. the abil ... | 1976 | 825415 |
| relationship of redox potential to the growth cycle of microorganisms. | | 1976 | 825453 |
| the identity of s15-1-a and b with racemomycins a and c. | | 1976 | 825497 |
| studies of etamycin biosynthesis employing short-term experiments with radiolabeled precursors. | the biosynthesis of etamycin by streptomyces griseoviridus was investigated with the precursors, 14c-l-leucine and 14ch3-l-methionine, during short-term incubations. both radiosotopes are rapidly incorporated into the antibiotic without significant lag. d-leucine,but not l-leucine,inhibits etamycin formation. the kinetics of incorporation of 14c-l-leucine as well as the inhibitor studies suggest that l-leucine is the direct precursor of thed-enantiomerpresent in the antibiotic peptide. chloramep ... | 1976 | 825498 |
| rubella hemagglutination-inhibition test: false-positive reactions in sera contaminated with bacteria. | we have shown that bacterial contamination of sera can have a marked influence on the results of the rubella hemagglutination-inhibition (hi) test. in addition to increasing the levels of nonspecific agglutinins, a number of common bacterial species tested had a significant effect on the hi titers. when sera free from rubella-specific antibodies were contaminated with bacillus subtilis or pseudomonas fluorescens, hi titers ranged from 16 to 128 (expressed as the reciprocal of the highest serum d ... | 1976 | 825529 |
| [contribution to the study of sterilisation of drugs presented as sprays (authors transl)]. | | 1976 | 825633 |
| characterization of iturin a in antibiotics from various strains of bacillus subtilis. | iturin a, an antifungal lipopeptide characterized by the presence of homologous liposoluble beta-aminoacids was found to be the active component to bacillomycin b, bacillomycin r and eumycin. iturin a was identified by thin-layer chromatography, aminoacid analysis and by characterization of liposoluble aminoacids and peptides. another two preparations: the antibiotic of raubitschek and the bacillomycin of landy et al. contain components of the same structural type but they are different from itu ... | 1976 | 825665 |
| adjuvant activity of bacterial glycolipids. | | 1976 | 825676 |
| [the stimulation of the competence in b. subtilis and its possible inhibition at different cell levels]. | | 1975 | 825701 |
| on the identity of dnap and dnaf genes of bacillus subtilis. | the dnap strains of bacillus subtilis are altered in the initiation of dna replication at high temperature (riva et al., 1975). fine mapping of the gene shows that it is located very close to the dnaf gene described by karamata and gross (1970) and mapped by love et al. (1976) in the polc region. the phenotype of both mutants is indistinguishable: the dna synthesis stops at non permissive temperature after synthesizing an amount of dna equivalent to the completion of the rounds of replication al ... | 1976 | 825719 |
| 5-methoxyuridine: a new minor constituent located in the first position of the anticodon of trnaala, trnathr, and trnaval from bacillus subtilis. | the sequences of the anticodon of trnaala, trnathr, and trnaval from bacillus subtilis w 168 were n-g-c, n-g-u, and n-a-c, respectively. a new minor constituent, n, occupied the first position of the anticodon of each trna. n was indentified as 5-methoxyuridine (mo5u, figure 1) by comparison of its uv absorption spectra, rf values in thin-layer chromatography using several solvent systems and mass spectra with those of chemically synthesized specimen. | 1976 | 825836 |
| [peptidases of preparations of proteolytic enzymes from several species of bacteria, molds and actinomycetes]. | the activity of carboxy- and aminopeptidases of enzymic preparations obtained from bacteria bacillus subtilis 1 m, 17, bacillus mesentericus 100/26, 100/56, 16; pseudomonas aeruginosa 1/7a, 1/4; bacillus thermophilicus s. sp. nov b-22, moulds aspergillus oryzae 1-746, aspergillus flavus 716 and actinomycete streptomyces griseus 32-13 was studied. in the bacterial preparations the proteolytic activity was 1.5-2.5 times lower than in the fungal and actinomycete preparations. the carboxypeptidase a ... | 1976 | 825850 |
| [effect of addition of bases and amino acids on inosine biosynthesis by mutants of bacillus subtilis]. | the relationship between the inosine biosynthesis by the bacillus subtilis mutants vnii-genetika-265 and vnii-genetika-21 and the concentrations of adenine, guanine, uracile and amino acids was investigated. adenine and guanine in high concentrations inhibited the inosine biosynthesis in both mutants. the mutant gene-21 showed an increased requirement for adenine as compared with the mutant gene-265. in the presence of uracile the pattern of the relationship between the inosine growth and biosyn ... | 1976 | 825851 |
| expression of the thymidylate synthetase gene of the bacillus subtilis bacteriophage phi-3-t in escherichia coli. | the thymidylate synthetase gene of b. subtilis bacteriophage phi-3-t, when cloned in plasmids psc101 or pmb9 is expressed in e. coli. the promoter of the cloned gene is likely to originate in phi-3-t. rearrangements of hybrid plasmid sequences during the cloning have been noted. b. subtilis strains can be transformed with hybrid dnas. the transformants contain sequences of phi-3-t, but not those of plasmid vectors. | 1976 | 825858 |
| bedding disposal cabinet for containment of aerosols generated by animal cage cleaning procedures. | laboratory tests with aerosolized spores and animal room tests with uranine dye indicate the effectiveness of a prototype bedding disposal cabinet in reducing airborne contamination generated by cage cleaning procedures. | 1976 | 826219 |
| base sequence complexity of the stable rna species of drosophila melanogaster. | the base sequence complexity of drosophila transfer rna (trna), 5s rna, and 18s + 28s ribosomal rna was determined by analyzing the kinetics of rna-dna hybridization on membrane filters. we find that drosophila trna is made up from about 59 basic nucleotide sequences distinguishable by hybridization, suggesting that many of the 99 trna species resolved by reverse phase chromatography (rpc-5) are homogenic. in contrast 5s rna was found to contain a single family of sequences. either 18s ribosomal ... | 1976 | 826268 |
| the folate-mediated synthesis of ribothymidylate in transfer ribonucleic acid, and evidence for multifunctional enzymes in one-carbon metabolism in eukaryotic sources. | | 1976 | 826434 |
| [mutants of bacillus subtilis--producer of alkaline proteinase, sporulating in the presence of high concentrations of glucose]. | glur mutants of bacillus subtilis a-50 capable of sporulating in the medium containing 5% glucose are isolated. the mutants are divided into three groups. the first group comprises glur mutants whose level of proteolytic activity is lower than that of the initial strain a-50. the productivity of mutants of the 2nd group is equal to the level of alkaline proteinase synthesis characteristics of the original strain. in mutants of the third group the level of alkaline protease synthesis exceeds the ... | 1976 | 826455 |
| [operon for riboflavin synthesis in bacillus subtilis. xi. determination of the type of regulation using a test for dominance of operator-constitutive and regulator-constitutive mutations]. | the strain gsy 468 of bacillus subtilis, giving persistent unstable merozogotes was used as recipient in transformation. diploid cells bearing a regulator constitutive or operator constitutive mutation were obtained, and their phenotype was studied. the operator constitutive mutation behaves as a dominant one, but the regulator constitutive is recessive. hence the regulation type in riboflavin operon is negative. the product of the regulator gene is obviously a repressor protein. | 1976 | 826456 |
| pola dependent repair of 5-bromouracil-labelled bacillus subtilis transforming dna irradiated with u.v. in the presence of cysteamine. | | 1976 | 826497 |
| biosynthesis of ribothymidine in the transfer rna of streptococcus faecalis and bacillus subtilis. a methylation of rna involving 5,10-methylenetetrahydrofolate. | the methyl moiety of ribothymidine in the trna of streptococcus faecalis, bacillus subtilis, and probably bacillus cereus is dervied from a 1-carbon folate derivative and not s-adenosylmethionine. micrococcus luteus (m. lysodeikticus) trna appears to be almost devoid of ribothymidine. s. faecalis trna lacking ribothymidine can be methylated in vitro with formation of ribothymidine. 5,10-methylenetetrahydrofolate has been identified as the 1-carbon donor involved in ribothymidine formation in s. ... | 1976 | 826533 |
| [nucleic acids utilized as the main source of bacterial nutrition]. | secretion of dnases and rnases, respectively, was found in saprophyte bacteria isolated from nature and growing on media containing dna and rna. serratia marcescens and bacillus subtilis with a high nuclease activity can assimilate rna and dna as the main source of nutrition. ser. marcescens with a nuclease which attacks both dna and rna can grow equally well on these acids. bac. subtilis has a higher activity of rnase and grows better on rna. | 1976 | 826761 |
| [inactivation of isogenous strains of bacillus subtilis and their mixtures by ionizing radiation]. | resistance to ionizing radiation of vegetative cells, spores and dehydrated cultures was studied with isogenous strains of bacillus subtilis with defects in the repair systems. in vegetative cells of different classes of the recombination deficient mutants, dl90 was 31-35% of ld90 of the wild type cells. differences in radiosensitivity of the vegetative cells were more pronounced cf. the spores and dehydrated cells: ld90 of rec- mutants was 55-88%; ld90 of uvr- mutants was 120% of ld90 of the pa ... | 1976 | 826767 |
| mechanism of dna synthesis inhibition by arabinosyl cytosine and arabinosyl adenine. | | 1976 | 826836 |
| a simplified procedure for the analysis of dna polymerase iii levels in bacillus subtilis strains. | a simple and reproducible procedure is described which allows the fast and almost quantitative removal of dna polymerases i and ii from dna polymerase iii, in crude extracts of pola+ strains of bacillus subtilis. the procedure entails streptomycin sulfate and ammonium sulfate fractionations; subsequent analysis of the partially purified preparation by g-200 chromatography, deae cellulose chromatography and density gradient sedimentation, shows that the ammonium sulfate fraction contains less tha ... | 1976 | 826885 |
| specific cleavage of chromatin by restriction nucleases. | digestion of mouse and rat liver nuclei with a restriction nuclease from bacillus subtilis (bsu) is examined in continuation of previous work from this laboratory (pfeiffer et al., 1975, nature 258, 450). the finding of more than 95% c in the 5'-termini of the dna fragments generated during digestion with bsu shows that the participation of endogenous nucleases in bsu digestion is extremely small. the restriction nuclease hae iii, an isoschizomer of bsu, yields identical degradation patterns. th ... | 1976 | 826886 |
| comparison of various procedures for removing proteins and nucleic acids from cell walls of bacillus subtilis. | several procedures were used in an attempt to prepare clean cell walls from bacillus subtilis. the results indicate that protein and nucleic acids are tightly bound to the walls. the cleanest wall preparations were found following trichloroacetic acid extraction at 60 degrees or by extraction with 0.1n naoh under a nitrogen atmosphere for 10 hrs. protein denaturants, such as sodium dodecyl sulfate and concentrated guanidine hydrochloride were relatively ineffective in removing proteins and nucle ... | 1976 | 826899 |
| [genetic transformation in the studies of dna replication and recombination (author's transl)]. | | 1976 | 826984 |
| [the traps of guthrie's test]. | presently, 90% of the newborn infants are undergoing screening for phenylketonuria before discharge from the maternity hospital. the tests of detection on urine (search for phenylpyruvic acid) and blood (evaluation of serum phenylalanine) have to be interpreted according to the fetal age, the age at sampling, the conditions of anabolism and the protid intake. | 1976 | 827271 |
| [pharmacokinetics of the trichomonacidal agent zk 25 095 in 6 normal subjects following intravenous and oral administration (author's transl)]. | the distribution, absorption and elimination of 5-morpholinomethyl-3-(5-nitro-1-methyl-2-imidazolyl-methylene-amino)-2-oxazolidinone-hydrochloride (test designation zk 25 095), which proved to be an effective trichomonacidal agent in animal and clinical trials, was determined microbiologically and with the aid of 14c-labelled substance. after i.v. injection of 10 mg zk 25095 in each of two volunteers the substance was distributed between blood and body tissues within approximately 10 min. the ca ... | 1976 | 827298 |
| the binding site of escherichia coli ribosomal protein s4 on 16-s ribosomal rna from different bacterial species. | ribosomal protein s4 of escherichia coli was bound to 16-s ribosomal rnas from several bacterial species and the complexes digested with pancreatic ribonuclease in an effort to isolate heterologous rna binding sites for protein s4. 16-s rnas from aeromonas punctata, pseudomonas fluorescens and vibrio cuneatus each gave rise to protected fragments whose electrophoretic mobility was 7s, i.e. similar to that of the fragment generated from e. coli 16-s rna using the same conditions. no comparable fr ... | 1976 | 827436 |
| transcription in vitro of phi29 dna and ecori fragments by bacillus subtilis rna polymerase. | ecori fragments a, b and c produced from linear phi29 dna, but not d or e fragments, are transcribed by purified bacillus subtilis rna polymerase. the transcription of fragments a and c is initiated preferentially with gtp and to a lesser extent with atp; the reverse happens in the case of fragment b. the dinucleotides gpu and gpa respectively, compete specifically with the incorporation of [gamma-32p]gtp directed by fragments a and c. the rna synthesized in vitro by purified b. subtilis rna pol ... | 1976 | 827446 |
| [uv-mutagenesis in bacillus subtilis. vi. mutagenesis under conditions of experimental delay of post-radiation dna replication]. | to test a suggestion that mutation frequency decline (mfd) in uv-irradiated bacteria starved by nitrogen sources is caused by long delay of the first postradiation cycle of dna replication, we used a strain 1201-05 of bacillus subtilis 168 (ade6 met5tsdnac) which does not synthesize dna at 42 degrees c. in a suspension of uv-irradiated cells of this strain which is incubated at 42 degrees in liquid deprived by nitrogen sources, a decrease of frequencies of ade+ and met+ revertants is observed. t ... | 1976 | 827462 |
| peptides from mycobacillin--negative sporeformer mutants of bacillus subtilis. | | 1976 | 827491 |
| rna polymerase in vegetative cells of bacillus subtilis. i. purification and properties of rna polymerase l1 and l2. | two forms of rna polymerase [ec 2.7.7.6], rpase l1 and rpase l2, isolated from a highly synchronized vegetative culture of bacillus subtilis marburg strain are described. rpase l1 is the major component (identical and the vegetative rna polymerase already reported) and rpase l2 is a minor, new component corresponding to 3--5% of the total activity. the enzymes differed in their requirements for divalent ions, though the differences depended on the template dna employed. prase l1 is able to trans ... | 1976 | 827546 |
| rna polymerase in vegetative cells of bacillus subtilis. ii. new polypeptide factors, fi and fii, stimulating in vitro rna synthesis directed by phage m2 dna. | two polypeptides named fi and fii were isolated from vegetative cells of bacillus subtilis marburg. the molecular weights of fi and fii were 15,000 and 30,000 daltons, respectively. they were able to stimulate the transcription of phage m2 dna in the presence of mg2+ ions by rpase l1 and rpase l2 [rna polymerase; ec 2.7.7.6]. although both core- and holo-rpase l2 hardly exhibited transcription activity under these conditions, the factors could stimulate both activities up to the level of rpase l ... | 1976 | 827547 |
| [disinfection of sewage waters from rendering plants by means by peracetic acid]. | in our experiments, peracetic acid -- known in commerce as "wolfasteril" was tested as a new and efficient disinfectant to disinfect sewage waters from rendering plants. peracetic acid was used in experiments in concentration of 0.1 to 1.0% for 30 sec. to 60 min. as a comparative agent, 5% chloramine was used. results obtained in preliminary and main experiments proved that peracetic acid is fully appropriate to disinfect biologically cleaned sewage waters in rendering plants. sewage waters supp ... | 1976 | 827581 |
| competence in continuous cultures of bacillus subtilis: inhibition by arginine and reversal of the inhibition by mn2+. | arginine inhibited the competence of bacillus subtilis growing in a chemostat at a dilution rate of 0-277 h-1. the biosynthesis of competence-stimulating activity was only partially repressed. the inhibitory effect may be due to an alteration in the cell's capacity for being stimulated to competence and/or in its ability to take up dna irreversibly. mnso4 at 10(-6) m restored competence immediately. | 1976 | 827601 |
| mapping of mutations affecting synthesis of exocellular enzymes in bacillus subtilis. identity of the sacuh, amyb and pap mutations. | the sacuh, amyb and pap mutations are identical with respect to their pleiotropic phenotype and their genetic location. strains bearing these mutations overproduce several exocellular enzymes: alpha amylase, lavansucrase and proteases, they are poorly or not at all transformable and most of them are devoid of flagella. these mutations are tightly linked to the sacu- mutations by transformation and therefore lie between the hisa1 and gtab290 markers. it is possible that the sacuh, amyb and pap mu ... | 1976 | 827683 |
| discrimination of competent bacillus subtilis with respect to ribonucleic acids. | a study has been made of the affinity of double-stranded helical rna for dna receptors in competent bacillus subtilis. in competition experiments, using homologous and heterologous dna samples which had been sheared to molecular weights comparable to that of the rna (about 2 x 10(6)), and which still exhibited appreciable competition in dna uptake experiments, the replicative form of phage f2 rna showed no evidence of affinity for receptor sites. a second double-stranded rna preperation from a w ... | 1976 | 827684 |