| identification of yersinia species by the api 20e. | a prospective study was performed to assess the effectiveness of the api 20e in the identification of 183 yersinia isolates incubated at 28 degrees c for 18 to 24 h. the results showed an overall correct-identification rate of 90%, with positive predictive values for yersinia enterocolitica and yersinia frederiksenii of 94 and 92%, respectively. yersinia intermedia results were unacceptable. | 1990 | 2199507 |
| concerns of microbial pathogens in association with dairy foods. | recent outbreaks of foodborne disease linked to salmonella, listeria, and yersinia have highlighted consumer awareness of microbiological problems in the food supply. such outbreaks affirm the need for improved testing, environmental monitoring, and epidemiological surveillance. this paper reviews the entry of microbial pathogens into foods, with an emphasis on dairy products, by examining the contribution of the processing environment to microbial contamination. numerous surveys, including a re ... | 1990 | 2200814 |
| yersinia enterocolitica immunodominant 60 kda antigen, common to a broad range of bacteria, is a heat-shock protein. | monoclonal antibodies (mabs) against the yersinia enterocolitica immunodominant 60 kda antigen, termed cross-reacting protein antigen (crpa), were obtained by fusion of spleen cells from mice immunized with crpa with murine myeloma cells. the reactivities of the mabs were examined by western blotting against extracts of y. enterocolitica and 23 other species of gram-positive and gram-negative bacteria. cross-reactions were recognized with a wide range of bacteria, but not with gram-positive cocc ... | 1990 | 2200844 |
| nucleotide sequence of yst, the yersinia enterocolitica gene encoding the heat-stable enterotoxin, and prevalence of the gene among pathogenic and nonpathogenic yersiniae. | the gene encoding the heat-stable enterotoxin (yst) was cloned from the chromosome of yersinia enterocolitica w1024 (serotype o:9), and the nucleotide sequence was determined. the yst gene encodes a 71-amino-acid polypeptide. the c-terminal 30 amino acids of the predicted protein exactly correspond to the amino acid sequence of the toxin extracted from culture supernatants (t. takao, n. tominaga, and y. shimonishi, biochem. biophys. res. commun. 125:845-851, 1984). the n-terminal 18 amino acids ... | 1990 | 2201642 |
| in vitro activity of ljc10,627, a new carbapenem antibiotic with high stability to dehydropeptidase i. | the in vitro activity of ljc10,627, a new carbapenem, was compared with those of imipenem and ceftazidime. ljc10,627 had broad-spectrum activity against gram-positive and gram-negative clinical isolates. the mics of this compound for 90% of members of the family enterobacteriaceae tested (mic90s), including strains resistant to ceftazidime, ranged from 0.1 to 25 micrograms/ml. ljc10,627 inhibited pseudomonas aeruginosa at an mic90 of 3.13 micrograms/ml; it thus was twofold more active than imipe ... | 1990 | 2203313 |
| positive immunostaining of ovine congenital progressive muscular dystrophy with antibody against n- and c-terminal dystrophin. | | 1990 | 2203334 |
| [yersinia-induced arthritis: new knowledge of pathogenesis, diagnosis and therapy]. | yersinia infections have been identified in rising incidence as the cause of acute or subacute intestinal or extraintestinal diseases in the past two decades. immunopathological manifestations of yersinia infections, e.g. yersinia-induced arthritis, have evoked special interest among clinicians and microbiologists. beneath epidemiological and clinical characteristics this review focuses on recent progress in pathogenesis and serological and immunohistological diagnosis of yersiniosis. possible c ... | 1990 | 2204212 |
| [clinico-allergic indicators in children with yersinia infection]. | | 1990 | 2204873 |
| determination of plasmid-associated hydrophobicity of yersinia enterocolitica by the salting-out test. | the hydrophobicity of the cell surface of yersinia enterocolitica was investigated by the salting-out test. it was shown that plasmid-containing strains grown at 37 degrees c aggregated at a final concentration of (nh4)2so4 of between 0.3 to 0.5 m. in contrast, the plasmid-free derivatives did not show aggregation even at a concentration of 2.0 m(nh4)2so4. therefore, the salting-out test can be utilized as a simple and reliable method for distinguishing plasmid-containing, virulent strains of ye ... | 1990 | 2206200 |
| yersiniosis. | | 1990 | 2208969 |
| involvement of m cells in the bacterial invasion of peyer's patches: a common mechanism shared by yersinia enterocolitica and other enteroinvasive bacteria. | recent evidence indicates that ileal peyer's patches represent the main infection route for yersinia enterocolitica. in this study transmission and scanning electron microscopy have shown that only a small fraction of bacteria present in the lumen adhere to the follicle-associated murine epithelium with no discernible preference for either m or absorptive cells. yersiniae attached to m cells are phagocytosed and transported from the lumen into the lamina propria. no invasion of columnar absorpti ... | 1990 | 2210445 |
| free amino acid turnover in methanogens measured by 15n nmr spectroscopy. | turnover of the nitrogen moiety from free amino acid pools in two thermophilic methanogens, methanobacterium thermautotrophicum delta h and methanococcus thermolithotrophicus sn1, has been monitored with 15n nmr spectroscopy. in cells growing exponentially on 15nh4cl, glutamate was the major soluble 15n-labeled species in both organisms. when the mb. thermoautotrophicum cells were harvested, washed, and resuspended into medium containing 14nh4cl, the resonance for [15n]glutamate decreased with a ... | 1990 | 2211697 |
| determination of dexamethasone in bovine tissues by coupled-column normal-phase high-performance liquid chromatography and capillary gas chromatography-mass spectrometry. | a new method for the determination of dexamethasone in bovine liver and muscle tissues has been developed. crude tissue extracts were obtained by means of a three-phase liquid-liquid extraction scheme. the resulting residue was subjected to coupled-column normal-phase high-performance liquid chromatography which served to isolate the drug for the purpose of screening and quantification. sample was injected onto the first column of the system, a phenyl column, from which a heart-cut was diverted ... | 1990 | 2211922 |
| use of urea filter paper disc to detect urease activity in enterobacteriaceae by multipoint replication techniques. | a new method of detecting urease activity in enterobacteriaceae was developed. an 8.5 cm filter paper disc impregnated with 20% urea and 0.5% bromocresol purple was placed on the surface of a glucose fermentation plate after inoculation with a multipoint replicator and overnight incubation. this method was compared with the commercially prepared mast urea agar (multipoint) and fuscoe's urea plate medium. a total of 240 routine isolates of enterobacteriaceae were tested for urease activity using ... | 1990 | 2212071 |
| self-instruction to prevent hiv infection among african-american and hispanic-american adolescents. | this study tested the efficacy of self-instruction intervention to reduce avoidable risks for hiv infection associated with drug use and unsafe sexual activity among african-american and hispanic adolescents (n = 60). after completing pretests, adolescent participants in the study were randomly divided into three conditions. participants in one condition received a self-instructional guide about aids and its transmission along with group instruction in using the guide. adolescents in another con ... | 1990 | 2212180 |
| the role of chemosensitive muscle receptors in cardiorespiratory regulation during exercise. | several possible mechanisms leading to the cardiorespiratory adjustments to muscular exercise can be considered. activation of the cardiovascular and respiratory centers may result from: (1) direct or reflex action of circulating metabolites (humoral control); (2) cortical influxes (central drive); (3) nervous impulses from receptors in the contracting muscles (peripheral drive). information presently available focuses most of the interest upon the muscular drive. our studies on anesthetized ani ... | 1990 | 2212481 |
| comparative study of the prevalence and clinical profiles of diarrheas due to aeromonas and other enteric pathogens. | the prevalence of aeromonas spp. and other enteric pathogens in stool specimens from diarrheic and non-diarrheic patients was studied over a 12 month period (january to december, 1986). except for the absence of fever, all the clinical features in aeromonas diarrhea were comparable to those associated with other diarrheagenic agents. these features included abdominal pain (30%), vomiting (24.5%), fever (31.5%), dehydration (9.5%) and hematochezia (19.5%). aeromonas spp. were more frequently isol ... | 1990 | 2212640 |
| effect of magnesium lithospermate b on urinary prostaglandins in rats with renal failure. | urinary excretion of prostaglandin e2 showed a significant increase following administration of magnesium lithospermate b [1] in rats with renal failure. in contrast, that of thromboxane b2 showed a significant decrease, indicating the improvement of renal failure. | 1990 | 2213036 |
| immunologic and pathologic manifestations of the infection of rhesus monkeys with simian immunodeficiency virus of macaques. | the striking similarities between simian immunodeficiency virus (siv)-induced disease in macaque monkeys and hiv-induced disease in humans make the siv-induced macaque monkey an extraordinarily important model for the study of aids. the most significant difference between these lentivirus-induced syndromes is the more rapid progression of disease in siv-infected monkeys. the immunologic and pathologic manifestations of siv infections in rhesus monkeys are described. | 1990 | 2213505 |
| yersinia enterocolitica serotype o:3 is arthritogenic for mice. | it is shown, for the first time, that yersinia enterocolitica serotype o:3 is experimentally arthritogenic. moreover, it is arthritogenic for the mouse, an optimal model for human yersiniosis. this arthritis can be induced by the oral route, the most common route in man. the pattern of joint disease closely parallels that of human reactive arthritis associated with this pathogen. | 1990 | 2215184 |
| homologous dna sequences on the virulence plasmids of pathogenic yersinia and salmonella dublin lane. | yersinia and salmonella harbour plasmids that encode traits important for virulence, enabling both pathogenic genera to survive and grow in cells of the reticulo-endothelial organs during systemic infections. we have detected dna homology between the salmonella dublin virulence plasmid psdl2 and the plasmids of the pathogenic yersinia species pestis, pseudotuberculosis, and enterocolitica. three regions of psdl2 were found to share homology with the virulence plasmid pib1 of yersinia pseudotuber ... | 1990 | 2215215 |
| precipitation of struvite in urine medium by urease-positive and urease-negative yersinia strains. | yersinia strains either urease-positive or urease-negative were examined for precipitation of struvite in human urine at 25 and 37 degrees c. all urease-positive strains and 8 out of 10 urease-negative strains showed the ability to produce these crystals. incubation time required for precipitation was longer for urease-negative strains and quantity of struvite formed was higher in urease-positive ones. regarding incubation temperature, no significant influence has been observed. | 1990 | 2219568 |
| [the occurrence of yersinia enterocolitica in sewage]. | using a modified cold enrichment procedure yersinia spp. were detected in 90.6% out of 32 raw waste water samples obtained within one year from two municipal sewage treatment plants. moreover yersinia were isolated from 50% of 6 effluent samples. altogether 118 yersinia strains were isolated and typed biochemically and serologically. 69 out of these isolates belonged to yersinia enterocolitica, 60 strains to biotype 1, and 9 to biotype 4, serotype 0:3, 8 strains yersinia enterocolitica serotype ... | 1990 | 2220165 |
| hemolysin as a marker for serratia. | all serratia marcescens strains (total of 33) of different sources were hemolytic including clinical strains previously classified as being nonhemolytic. dna fragments of the two hemolysin genes hybridized with the chromosomal dna of s. marcescens, s. liquefaciens, s. kiliensis, s. grimesii, s. proteamaculans, s. plymutica, s. rubridaea which were also hemolytic. the restriction pattern of the hemolysin locus differed in each strain. s. ficaria and s. marinorubra expressed a different hemolysin ... | 1990 | 2222120 |
| production and response of a human prostatic cancer line to transforming growth factor-like molecules. | serum-free media conditioned by the androgen insensitive human prostate cancer cell line du145 showed immunological transforming growth factor-alpha (tgf alpha) activity, as well as competing activity in epidermal growth factor (egf) radioreceptor assays (rra). furthermore, there were factors in the conditioned media which inhibited and stimulated dna synthesis by du145 cells in a dose-dependent fashion. fractionation of the concentrated conditioned media by reverse-phase high performance liquid ... | 1990 | 2223575 |
| production and response of a human prostatic cancer line to transforming growth factor-like molecules. | serum-free media conditioned by the androgen insensitive human prostate cancer cell line du145 showed immunological transforming growth factor-alpha (tgf alpha) activity, as well as competing activity in epidermal growth factor (egf) radioreceptor assays (rra). furthermore, there were factors in the conditioned media which inhibited and stimulated dna synthesis by du145 cells in a dose-dependent fashion. fractionation of the concentrated conditioned media by reverse-phase high performance liquid ... | 1990 | 2223575 |
| glucose partitioning in the pregnant ewe: effects of undernutrition and exercise. | maternal whole-body glucose entry rate and uterine and umbilical net uptakes of glucose and oxygen were measured in single-pregnant ewes which were either well-fed throughout, or fed at 0.3-0.4 predicted energy requirement for 7-21 d during late pregnancy. all ewes were studied while standing at rest and then while walking on a treadmill at 0.7 m/s on a 10 degrees slope for 60 min. underfed ewes suffered significant decreases in live weight and had lower fetal, but not placental, weights at 140- ... | 1990 | 2223746 |
| glucose partitioning in the pregnant ewe: effects of undernutrition and exercise. | maternal whole-body glucose entry rate and uterine and umbilical net uptakes of glucose and oxygen were measured in single-pregnant ewes which were either well-fed throughout, or fed at 0.3-0.4 predicted energy requirement for 7-21 d during late pregnancy. all ewes were studied while standing at rest and then while walking on a treadmill at 0.7 m/s on a 10 degrees slope for 60 min. underfed ewes suffered significant decreases in live weight and had lower fetal, but not placental, weights at 140- ... | 1990 | 2223746 |
| yersinia enterocolitica abscess of the transverse colon. report of a case. | yersinia enterocolitica abscess of the bowel is a rare entity. only five cases have previously been reported--none in the surgical literature. a unique presentation for yersinia infection, abscess of the transverse colon, is described, and the literature of y. enterocolitica intestinal perforation and abscess is reviewed. | 1990 | 2226090 |
| evaluation of different techniques for detection of virulence in yersinia enterocolitica. | | 1990 | 2229402 |
| sequence, localization and function of the invasin protein of yersinia enterocolitica. | the inv locus of yersinia enterocolitica is sufficient to convert a non-invasive escherichia coli k12 strain into a microorganism that is able to penetrate cultured mammalian cells. the nucleotide sequence of inv reveals an open reading frame corresponding to an 835-amino-acid protein that is homologous to the invasin protein from yersinia pseudotuberculosis. a polyclonal antiserum elicited by a synthetic peptide corresponding to the c-terminal 88 amino acids of this open reading frame detected ... | 1990 | 2233250 |
| [the capacity for regeneration of the myocardium in rat fetuses]. | a reproducible model of heart injury in 16-days old rat fetuses has been developed. 30 days after the injury a 4-fold increase of the damaged area was observed. decrease of reparative capacity of myocardium in fetuses as compared with the adults is due to the functional immaturity of the connective tissue cells. | 1990 | 2234794 |
| halophilic proteins and the influence of solvent on protein stabilization. | competition between protein-solvent and protein-protein interactions is arguably the most important contributing factor to polypeptide folding in general. a study of halophilic proteins, correlating their stability and solution structures in different conditions, focuses on the effects of a high salt solvent. a mechanism is proposed to explain how these proteins have adapted to such an extreme environment. | 1990 | 2238041 |
| halophilic proteins and the influence of solvent on protein stabilization. | competition between protein-solvent and protein-protein interactions is arguably the most important contributing factor to polypeptide folding in general. a study of halophilic proteins, correlating their stability and solution structures in different conditions, focuses on the effects of a high salt solvent. a mechanism is proposed to explain how these proteins have adapted to such an extreme environment. | 1990 | 2238041 |
| the influence of properties encoded by the yersinia virulence plasmid on adhesion of yersinia enterocolitica to ileal brush border membrane vesicles. | the influence of plasmid-associated cell surface structures on the ability of yersinia enterocolitica to bind to ileal brush border membrane vesicles (bbvs) was investigated. rabbit or human bbvs were immobilized on polystyrene microtiter plates and adhesion of radiolabeled cells of y. enterocolitica was determined. strains of pathogenic serotypes carrying the yersinia virulence plasmid (pyv+), as well as their isogenic plasmid cured derivatives (pyv-), adhered to immobilized bbvs, but adhesion ... | 1990 | 2245012 |
| the influence of ph, temperature and organic acids on the initiation of growth of yersinia enterocolitica. | the influence of incubation temperature, and of acetic, lactic and citric acids on the minimum ph for the initiation of growth of six strains of yersinia enterocolitica was determined. the strains included two of serotype o : 9, two of serotype o : 3, and one each of serotypes o : 8 and o : 5, 27. in a culture medium acidified with hcl to ph values between 4.0 and 6.0 at intervals of approximately 0.1 unit the minimum ph at which growth was detected after incubation at 20 degrees, 10 degrees, 7 ... | 1990 | 2246144 |
| morphine attenuation of a conditioned emotional response in post-dependent rats. | rats placed in a test chamber where they had received repetitive shocks the previous day significantly reduced their motor activity; this was taken as indicator of a conditioned emotional response. morphine attenuated this conditioned suppression of motility, possibly due to a reduction of the anxiety associated with the expectation of the noxious stimuli. previous morphine dependence (20 mg/kg daily for 26 days) did not modify the effect of the opioid on the conditioned suppression of motility. ... | 1990 | 2253691 |
| in-vitro activity of cefotaxime against clinical isolates of yersinia enterocolitica biotype 4, serotype 03. | | 1990 | 2254230 |
| detection of pyv+ yersinia enterocolitica isolates by p1 slide agglutination. | rabbit polyclonal antisera were raised against the pyv-encoded outer membrane protein p1 of five yersinia enterocolitica strains belonging to serogroups o:3, o:5,27, o:8, and o:9. analysis of these strains with the sera showed that p1 presented at least six different antigenic factors. two of the serum specimens were chosen to test the p1 agglutinability of 797 strains isolated from various sources. this technique appeared to be more reliable than autoagglutination and ca2+ dependency to monitor ... | 1990 | 2254416 |
| mice and moles inhabiting mountainous areas of shimane peninsula as sources of infection with yersinia pseudotuberculosis. | a total of 1,835 yersinia spp. were isolated from 925 (60.5%) of 1,530 wild mice and from 139 (79.9%) of 174 moles living in mountainous areas of eastern shimane prefecture, japan. the yersinia spp. included 1,106 yersinia enterocolitica, 26 y. enterocolitica-like, 176 yersinia mollaretii, 149 yersinia frederiksenii, 70 yersinia intermedia, 231 yersinia kristensenii, 5 yersinia aldovae, and 72 yersinia pseudotuberculosis. human pathogenic y. enterocolitica was not isolated. y. pseudotuberculosis ... | 1990 | 2254420 |
| yersinia enterocolitica tenosynovitis. the first case. | we describe a case of tenosynovitis of tibialis posterior due to yersinia enterocolitica occurring after injury by a plant thorn in a 55-year-old man. the illness was chronic with 2 recurrences in spite of antibiotic treatment. full recovery was obtained only after surgical intervention. our patient's chronic course was fostered by the persistence of thorn fragments in the infected area and the exceptionally pathogenic character of the isolated colony of yersinia. | 1990 | 2254906 |
| yersinia enterocolitica bacteraemia after blood transfusion. | | 1990 | 2255295 |
| [the differential diagnosis of acute intestinal infections]. | the authors evaluate contemporary possibilities of clinico-epidemiological and laboratory diagnosis of intestinal yersiniosis in the clinical practice. data are reported on infestation of the environment with yersiniosis pathogen in this territory and the clinical picture of gastrointestinal form of yersiniosis and its symptomatology that is of help in the diagnosis. perfection of laboratory diagnosis is of great importance in this respect. | 1990 | 2256268 |
| lymphoid dendritic accessory cells of the rat. | the expression of mhc class ii antigens on potential apc is a crucial step in t-lymphocyte activation and the initiation of an immune response. the studies which are presented here were initiated to characterize the critical apc present in physiologically normal, untreated rats. such a cell should constitutively express these antigens at high density and therefore provide the apparatus necessary to provoke both primary and secondary immune responses at any time. dac were found to fulfill these c ... | 1990 | 2258188 |
| [detection of toxigenic vibrio cholerae o1 using polymerase chain reaction for amplifying the cholera enterotoxin gene]. | a rapid and simple procedure using polymerase chain reaction (pcr) was developed for the detection of cholera enterotoxin (ct) producing character. this method is based on amplifying a 380 base pair (bp) segment of the ct gene (ctx) which controls the production of ct. two single-stranded oligonucleotides, synthetized to be complementary to the known nucleotide sequences of genes encoding the a-subunit of ctx, were used as extension primers. the oligonucleotide sequences are 5'tcaaactatattgtctgg ... | 1990 | 2258648 |
| [detection of toxigenic vibrio cholerae o1 using polymerase chain reaction for amplifying the cholera enterotoxin gene]. | a rapid and simple procedure using polymerase chain reaction (pcr) was developed for the detection of cholera enterotoxin (ct) producing character. this method is based on amplifying a 380 base pair (bp) segment of the ct gene (ctx) which controls the production of ct. two single-stranded oligonucleotides, synthetized to be complementary to the known nucleotide sequences of genes encoding the a-subunit of ctx, were used as extension primers. the oligonucleotide sequences are 5'tcaaactatattgtctgg ... | 1990 | 2258648 |
| [yersinia enterocolitica: investigation in 1,300 children under 5 years of age with acute diarrhea]. | the presence of yersinis enterocolitica was investigated in feces of 1,300 children younger than 5 years, hospitalized because of presenting acute diarrheic disease in cuba during 1985-1988 period at different provincial pediatric hospital, as well as in havana city. selective agar base for yersinia was used supplemented with cephsuloidin, irgasan and novoviocin (cin) and buffered saline solution as enrichment medium. this last one at 4 degrees c during 21 days with weekly resowings to the selec ... | 1990 | 2259775 |
| yersinia enterocolitica in the synovial membrane of patients with yersinia-induced arthritis. | using a monospecific rabbit antibody against yersinia enterocolitica outer membrane protein 1, we examined synovial biopsy specimens from 7 patients with yersinia-induced arthritis. yersinia were demonstrated in the synovial membrane by indirect immunofluorescence in 4 patients with yersinia-induced arthritis, but not in 6 control patients with salmonella-induced arthritis or with rheumatoid arthritis. these findings suggest the persistence of yersinia in the joints of patients with yersinia-ind ... | 1990 | 2261001 |
| proliferative response of synovial fluid and peripheral blood mononuclear cells to arthritogenic and non-arthritogenic microbial antigens and to the 65-kda mycobacterial heat-shock protein. | cellular immune responses to microbial antigens have been implicated in the pathogenesis of some forms of arthritis including reactive arthritis, reiter's syndrome, ankylosing spondylitis and rheumatoid arthritis. we investigated the proliferative t cell responses of paired peripheral blood (pb) and synovial fluid (sf) mononuclear cells (mc) to so-called arthritogenic bacteria (yersinia enterocolitica and salmonella typhimurium), to control antigens, such as candida albicans, mumps virus and pur ... | 1990 | 2263227 |
| control of choroidal blood flow by the nucleus of edinger-westphal in pigeons: a laser doppler study. | anatomical studies in birds have suggested that choroidal blood flow may be regulated by a circuit involving the following serially-connected components: the retina-the suprachiasmatic nucleus (scn)-the medial subdivision of the nucleus of edinger-westphal (mew)-the ciliary ganglion-the choroidal blood vessels. in order to better clarify the role of this circuit, we examined the effects of electrical stimulation of ew on choroidal blood flow in the ipsilateral eye, using laser doppler velocimetr ... | 1990 | 2265988 |
| a model to study physiological activation of phospholipase a2 and vasorelaxation by lysophosphatidylcholine. | earlier we demonstrated that micellar solutions of lpc caused endothelium-dependent relaxation of rabbit thoracic aorta and bovine intrapulmonary artery and vein through a cyclic gmp-dependent mechanism. the availability of lpc for vasorelaxation depends on its production by deacylation of pc by pla2. we assessed the possible activation of pla2 by commonly used vasorelaxants such as acetylcholine, bradykinin, calcium ionophore a23187 and thrombin and vasoconstrictors like histamine and phenyleph ... | 1990 | 2266777 |
| fate of injected glucagon taken up by rat liver in vivo. degradation of internalized ligand in the endosomal compartment. | the uptake and processing of glucagon into liver endosomes were studied in vivo by subcellular fractionation. after injection of [[125i]iodo-tyr10]glucagon and [[125i]iodo-tyr13]glucagon to rats, the uptake of radioactivity into the liver was maximum at 2 min (6% of the dose/g of tissue). on differential centrifugation, the radioactivity in the homogenate was recovered mainly in the nuclear (n), microsomal (p) and supernatant (s) fractions, with maxima at 5, 10 and 40 min, respectively; recovery ... | 1990 | 2268296 |
| the preferential uptake of very-low-density lipoprotein cholesteryl ester by rat liver in vivo. | the removal from the blood and the uptake by the liver of injected very-low-density lipoprotein (vldl) preparations that had been radiolabelled in their apoprotein and cholesteryl ester moieties was studied in lactating rats. radiolabelled cholesteryl ester was removed from the blood and taken up by the liver more rapidly than sucrose-radiolabelled apoprotein. near-maximum cholesteryl ester uptake by the liver occurred within 5 min of the injection of the vldl. at this time, apoprotein b uptake ... | 1990 | 2268298 |
| the role of the 5'-flanking sequence of a human trna(glu) gene in modulation of its transcriptional activity in vitro. | the role of a trna-like structure within the 5'-flanking sequence of a human trna(glu) gene in the modulation of its transcription in vitro by hela cell extracts has been investigated using several deletion mutants of a recombinant of the gene which lacked part or all of the trna-like structure. the transcriptional efficiency of four mutants was the same as that of the wild-type recombinant, two mutants had decreased transcriptional efficiency, one was more efficient, and one, lacking part of th ... | 1990 | 2268303 |
| proteolytic processing of porcine big endothelin-1 catalyzed by cathepsin d. | cathepsin d, a candidate for endothelin-converting enzyme (ece), was allowed to act on porcine big endothelin-1 (big et-1, 1-39). the proteinase primarily cleaved the asp18-ile19 and trp21-val22 bonds of big et-1(1-39), with a optimum ph of 3.5. the mature et-1(1-21), generated by the cleavage between trp21 and val22, was subsequently degraded by removal of most of the c-terminal tripeptide (ile19-ile20-trp21). therefore, cathepsin d is by no means a specific ece, although the proteinase does cl ... | 1990 | 2268325 |
| survey of enteropathogenic agents in children with and without diarrhoea in ghana. | a survey was carried out over 1 year in a rural area of ghana on the isolation, detection and/or identification of enteric pathogens from children under 5 years of age with and without diarrhoea. the isolation and detection rate of shigella flexneri, shigella dysenteriae, giardia lamblia and rotavirus were higher in children with diarrhoea than in controls. yersinia enterocolitica, vibrio cholerae and vibrio parahaemolyticus were not isolated during the period of this survey. the incidence of ot ... | 1990 | 2270006 |
| [from the parenchyma to the mucosa biopsy: significance and importance of local assays of antibiotics. a critical study]. | the authors discussed the relevance of antibiotic concentrations as measured in tissues and fluids of the respiratory tract. they analysed theoretical factors which influence tissue distribution of antibiotics as well as the results of studies carried out in animal and in human tissues. various factors may interfere with the results of antibiotic assays: but the data published by various groups were usually convergent: this confirms the significance of "tissue pharmacokinetics". new protocols ba ... | 1990 | 2270342 |
| in vivo t1 measurements of canine brain for deep tissue temperature measurements with mri. | the possibility of deep tissue temperature imaging with a magnetic resonance (mr) imager was studied in 30 mongrel dogs. the coronary view of the brain of a dog anesthetized with muscle relaxant and pentobarbital and ventilated automatically with air was taken with a special head coil by a 0.1 tesla mr imager, asahi mark-j, driven by a spin warp pulse sequence. t1s in the imaging of the right and left white matter, grey matter, thalamus and caudate nucleus were measured with rectal temperature i ... | 1990 | 2271416 |
| kinetic mechanism of orotate phosphoribosyltransferase from salmonella typhimurium. | the chemical mechanism of the phosphoribosyltransferases (prtases), although largely unknown, may proceed either via a concerted direct-transfer mechanism or with a two-step mechanism involving a carboxonium-like intermediate. to study this question, we have cloned the salmonella typhimurium pyre gene, coding for the enzyme orotate phosphoribosyltransferase (ec 2.2.4.10, oprtase), and developed a bacterial strain that overproduces the enzyme, which we have purified to homogeneity. initial veloci ... | 1990 | 2271660 |
| affinity labeling of aminoacyl-trna synthetases with adenosine triphosphopyridoxal: probing the lys-met-ser-lys-ser signature sequence as the atp-binding site in escherichia coli methionyl-and valyl-trna synthetases. | pyridoxal 5'-triphospho-5'-adenosine (ap3-pl), the affinity labeling reagent specific for lysine residues in the nucleotide-binding site of several enzymes [tagaya, m., & fukui, t. (1986) biochemistry 25, 2958-2964; yagami, t., tagaya, m., & fukui, t. (1988) febs lett. 229, 261-264], was used to identify the atp-binding site of escherichia coli methionyl-trna synthetase (metrs). incubation of this enzyme with ap3-pl followed by reduction with sodium borohydride resulted in a rapid inactivation o ... | 1990 | 2271710 |
| early antibiotic treatment of reactive arthritis associated with enteric infections: clinical and serological study. | to find out whether a 10-14 days' course of antibiotics early in the course of reactive arthritis associated with enteric infections could reduce the severity and duration of the disease and whether the antibody response in patients with reactive arthritis associated with yersinia infection differed between those treated and those not treated with the antibiotics. | 1990 | 2271852 |
| yersinia enterocolitica: intestinal features. | | 1990 | 2272121 |
| milk and plasma concentrations of the progestin st-1435 in women treated parenterally with st-1435. | a subcutaneous contraceptive capsule releasing progestin st-1435 was used by 6 breast-feeding women. one to three paired milk and plasma samples were collected over a one-month period and the concentrations of st-1435 were determined by radioimmunoassay. an improved and sensitive method for determination of st-1435 in milk was developed. a column chromatographic purification of milk prior to radioimmunoassay decreased the blank and improved sensitivity. the average plasma concentration of st-143 ... | 1990 | 2272183 |
| [serologic diagnosis and follow-up in patients with uveitis. antibodies to virulence antigens of yersinia enterocolitica]. | sera from 72 patients with uveitis and sera from 93 healthy blood donors were analyzed by an immunoblot technique for antibodies to plasmid-encoded virulence associated antigens of yersinia enterocolitica. in the group of patients with acute anterior uveitis (n = 16), antibodies of the classes iga, igg, igm were significantly increased in comparison to the healthy blood donors. the presence of iga antibodies is a sign of acute or persistent yersinia infection. for the other forms of uveitis thes ... | 1990 | 2272579 |
| [yersinia enterocolitica infection in a patient with thalassemia major]. | | 1990 | 2275497 |
| yersinia enterocolitica aroa mutants as carriers of the b subunit of the escherichia coli heat-labile enterotoxin to the murine immune system. | plasmid p5f which directs the expression of the escherichia coli heat-labile enterotoxin b subunit (lt-b) from the ptac promoter was introduced into the attenuated yersinia enterocolitica o:8 aroa mutant strain yam.1. yam.1 (p5f) expressed high levels of cell-associated and secreted lt-b in a stable fashion when grown on normal laboratory medium. the strain was used as a live oral vaccine in balb/c mice and vaccinated mice developed high levels of gut-associated and systemic antibodies to both l ... | 1990 | 2277586 |
| [influence of fluoride on induced bone formation and mineralization]. | by using undecalcified bone sections, accompanied by tetracycline double labeling and bone histomorphometry, the influence of different concentrations of fluoride on induced ectopic bone formation and mineralization during the 21th-30th day of the experiment was observed. results showed that on the 30th day, the induced bone formation in the group of 50ppm naf was more active than that of the control; and from the 21th day to the 26th day as well as from the 26th day to the 30th day, no inhibiti ... | 1990 | 2279310 |
| endoscopic findings in yersinia enterocolitica enterocolitis. | the endoscopic findings in the colon and terminal ileum in eight cases of yersinia enterocolitica enterocolitis infection were studied. the diagnosis was based on the isolation of y. enterocolitica in the feces and/or elevated serum antibody titers to the organism. total colonoscopy was performed between 7 and 38 days (mean, 24 days) after the onset of symptoms. in all patients, the terminal ileum was affected, followed by frequent involvement of the ileocecal valve and the cecum, and less frequ ... | 1990 | 2279647 |
| characterisation of yersinia enterocolitica strains associated with ovine abortion. | | 1990 | 2281589 |
| [a serological study of yersinia enterocolitica]. | in this study, the incidence of yersinia enterocolitica was investigated at neighbourhood of bursa and 13.26 percentage seropositivity was determined. | 1990 | 2283972 |
| rapid methods for differentiating gram-positive from gram-negative aerobic and facultative anaerobic bacteria. | different tests based on lysis by koh and on reaction with fluorogenic and chromogenic substrates, l-alanine-4-nitroanilide (lana); l-alanine-4-methoxy- beta-naphthylamide (mna); 4-alanine-2-amidoacridone (aaa); l-alanine-7-amido- 4-methylcoumarin (aamc); 8-anilino-1-naphthalene-sulphonic acid (ans) were compared for their suitability to distinguish gram-positive from gram-negative bacteria. a concentration of 100 micrograms/ml was chosen for incorporating lana, aaa, aamc and ans into the growth ... | 1990 | 2286581 |
| the pyv plasmid of yersinia encodes a lipoprotein, ylpa, related to trat. | a series of lipoproteins was detected in the membrane fraction of yersinia enterocolitica w227, a typical strain from serotype o:9. at least two of them, ylpa and ylpb, are encoded by the pyv plasmid. the sequence of ylpa reveals the presence of a typical lipoprotein signal peptide. the mature ylpa protein would be 223 residues long with a calculated molecular weight of 23798 for the proteic moiety of the molecule. ylpa shares 88% identical residues with the trat protein encoded by plasmid ped20 ... | 1990 | 2287280 |
| chemical modification of pig liver initiation factor eif-2 with n-ethylmaleimide. amino acid sequences around the n-ethylmaleimide-reactive sulfhydryl groups and the effect of gdp on the modification. | the activity of eukaryotic initiation factor eif-2 as to the formation of the ternary complex, eif-2 gtp met-trna(f), is inhibited by n-ethylmaleimide. our preparation of pig liver eif-2 contained alpha and gamma subunits and was inhibited by more than 90% by n-ethylmaleimide. using our eif-2, we determined the sequences around the n-ethylmaleimide-reactive sulfhydryl groups, studied the effect of gdp on the sulfhydryl modification and that of nem on the [3h]gdp binding, and examined the protect ... | 1990 | 2292592 |
| a model of multivessel coronary artery disease using conscious, chronically instrumented dogs. | the present investigation was undertaken to develop a reproducible model of multivessel coronary artery disease including a total occlusion of one vessel and a stenosis of an adjacent vessel in chronically instrumented dogs. utilizing sterile techniques, we surgically prepared dogs for measurement of heart rate, arterial pressure, left ventricular pressure, and blood flow velocity in the left anterior descending (lad) and left circumflex (lcca) coronary arteries. a hydraulic occluder and ameroid ... | 1990 | 2292881 |
| [age-related features of cardiac contractility and its adrenergic regulation in catecholamine damage to the myocardium]. | studies on isolated rat hearts showed that in contrast to 8-month-old animals, in 26-month-old rats the contractile activity of the myocardium increases by the 3rd day after administration of toxic adrenalin doses (1 mg/kg i.m.). the maximum functional response of catecholamine injured hearts to stimulation of alpha- and beta-adrenoceptors reduced in both age groups, while the sensitivity of the myocardium to beta-adrenoceptor agonists in mature rats increased. | 1990 | 2293152 |
| [drainage of the thoracic duct and changes in myocardial lymph drainage under conditions of electrothermocoagulation of the coronary artery]. | it was demonstrated in dog experiments that the drainage function of the heart lymphatic system and coagulation of the central lymph are disturbed in acute myocardial infarction. drainage of the thoracic duct with diversion of the flow of the toxic lymph fails to correct the diminished rate of lymph flow in the myocardium and does not prevent the increase of the hypercoagulation shift in the lymph. it is concluded that lymph stimulation and correction of the disorder of the lymph coagulation pot ... | 1990 | 2293154 |
| production of specific monoclonal antibodies to salmonella typhi flagellin and possible application to immunodiagnosis of typhoid fever. | four murine monoclonal antibodies (mabs) to salmonella typhi flagellin were produced. these mabs did not react with eight other enterobacterial strains tested: salmonella enteritidis, salmonella typhimurium, salmonella paratyphi a, escherichia coli, shigella flexneri, shigella sonnei, yersinia enterocolitica, and campylobacter jejuni. all four mabs cross-reacted with salmonella muenchen flagellin indicating specificity for d antigenic flagellar epitope. one mab (c4) was selected to develop a dou ... | 1990 | 2295860 |
| adhesion of yersinia enterocolitica to human epithelial cell lines and to rabbit and human small intestinal tissue. | in assays to determine adherence of yersinia enterocolitica, virulence plasmid-containing (pyv+) strains and strains of their plasmid-cured (pyv-) derivatives adhered equally efficiently to hela cells and fetal intestinal epithelial int 407 cells. non-pathogenic strains of y. enterocolitica did not adhere. in contrast, pyv+ strains adhered more efficiently to rabbit and human intestine than did pyv(-)-strains, as evaluated by an in vitro assay, measuring adhesion of radiolabeled bacteria to disk ... | 1990 | 2302342 |
| in vitro activity of norfloxacin and other antibacterial agents against gastro-intestinal pathogens isolated in sweden. | the in vitro activity of norfloxacin was compared to that of ampicillin, doxycycline, chloramphenicol, trimethoprim in combination with sulfamethoxazole (1/20), and erythromycin, against 272 clinical isolates of gastro-intestinal pathogens. norfloxacin was the most active compound of those tested with mics in the range 0.004-2 mg/l. concentrations inhibiting 90% of the strains (mic 90) were 0.008 mg/l for vibrio cholerae, 0.016 mg/l for aeromonas hydrophila, 0.032 mg/l for vibrio cholerae non 01 ... | 1990 | 2302351 |
| genetic analysis of the yope region of yersinia spp.: identification of a novel conserved locus, yera, regulating yope expression. | the yope gene of yersinia pseudotuberculosis was recently sequenced, and yope was identified as an indispensable virulence determinant when tested in a mouse model (a. forsberg and h. wolf-watz, mol. microbiol. 2:121-133, 1988). in the study described here, the dna sequences of the yope genes of yersinia pestis ev76 and yersinia enterocolitica 8081 were determined and compared with that of the y. pseudotuberculosis gene. only two codons were found to differ, both leading to amino acid replacemen ... | 1990 | 2307658 |
| a synthetic oligonucleotide probe and a cloned polynucleotide probe based on the yopa gene for detection and enumeration of virulent yersinia enterocolitica. | we compared a synthetically produced 19-mer oligonucleotide probe with a polynucleotide probe consisting of a cloned fragment of the virulence gene yopa for their relative efficiencies in identification and enumeration of virulent yersinia enterocolitica. the probes were used in dna-dna colony hybridization assays to differentiate 70 yersinia strains with known plasmid profiles. all 19 strains harboring the 40- to 50-megadalton virulence plasmid were positive in the hybridization assay, whereas ... | 1990 | 2310179 |
| hypokalemia and quadriparesis. | | 1990 | 2310287 |
| synovial fluid antigen-presenting cells unmask peripheral blood t cell responses to bacterial antigens in inflammatory arthritis. | we and others have previously shown that synovial fluid (sf) mononuclear cells (mc) from patients with both reactive arthritis and other inflammatory arthritides proliferate in vitro in response to bacteria clinically associated with the triggering of reactive arthritis. in all cases, such sfmc responses are greater than the corresponding peripheral blood (pb) mc responses, often markedly so, and the mechanism for this is unclear. we have investigated this phenomenon by comparing the relative ab ... | 1990 | 2311298 |
| hematogenous osteitis due to yersinia enterocolitica. | | 1990 | 2313139 |
| yersinia enterocolitica o:3 infections in infants and children, associated with the household preparation of chitterlings. | | 1990 | 2314448 |
| transfusion-associated yersinia enterocolitica. | | 1990 | 2315991 |
| sepsis associated with transfusion of red cells contaminated with yersinia enterocolitica. | between april 1987 and may 1989, the centers for disease control investigated seven cases of transfusion-associated yersinia enterocolitica sepsis; four were caused by organisms of serotype o:3, and one each was caused by organisms of serotype o:1,2,3; o:5,27; and o:20. all seven recipients developed septic shock after receiving units of red cells (rbcs) contaminated with y. enterocolitica; five recipients died. the cases occurred in seven states and were unrelated. there was no evidence for con ... | 1990 | 2315994 |
| asymptomatic yersinia enterocolitica infections during an outbreak in a day-nursery. | | 1990 | 2318220 |
| tibenelast, 5,6-diethoxybenzo(b)thiophene-2-carboxylic acid, sodium salt (ly186655), is an orally active anti-asthma compound in the guinea pig. | anaphylactic shock was induced in actively sensitized guinea pigs by free inhalation of a high dose of ovalbumin (10 mg/ml) aerosol. tibenelast (ly186655), 5,6-diethoxybenzo(b)-thiophene-2-carboxylic acid, sodium salt, proved to be a potent orally active compound against anaphylactic shock induced by high dose antigen aerosol. when a lower aerosol challenge (0.05 mg/ml) was employed, bronchoconstriction was observed with a concomitant increase in lung resistance (rl) and a fall in dynamic compli ... | 1990 | 2329918 |
| use of vicryl (polyglactin 910) mesh to limit epidural scar formation after laminectomy. | a variety of substances have been used at laminectomy sites to prevent postoperative epidural scarring. free grafts of autologous subcutaneous fat are commonly used both clinically and experimentally. the free fat grafts usually survive, but decrease in size by about 50%. postoperatively, subcutaneous seroma has been observed with the use of fat grafts, as well as recurrent symptoms of neural compression by the graft that required additional operations. when compared to the use of free grafts af ... | 1990 | 2330087 |
| comparative study of culture methods to detect yersinia enterocolitica serogroup o3 on swine tongues. | swine tongues were used as a model for evaluation of culture methods for y. enterocolitica o3. the following three methods were used: method a consisting of primary culture on dc, mcconkey, cin and ssd agar with additional long-term cold enrichment in pbs and/or selenite broth. method b--2 min, treatment of samples with 0.5 per cent koh solution followed by the same cultivation as above and method c--"cold shock" i.e. incubation of swabs without any medium at -20 degrees c overnight and then ino ... | 1990 | 2331297 |
| a highly efficient electroporation system for transformation of yersinia. | the various pathogenic yersinia species are not readily and efficiently transformed by classical methods. for this reason, the electroporation technique was applied for genetic transformation of these species. using optimal conditions, we were able to transform the six yersinia strains studied with the two most widely used groups of plasmids: psu2718 (a pacyc184 derivative) and pk19 (a puc19 derivative). only yersinia enterocolitica (y. e.) serotype 0:8 gave poor results (less than 5 x 10(2) tra ... | 1990 | 2332165 |
| simple assay of calcium dependency for virulent plasmid-bearing clones of yersinia enterocolitica. | a simplified procedure to detect the calcium dependency of virulent plasmid-bearing strains of yersinia enterocolitica was developed. a low-calcium, agarose-based medium of brain heat infusion with added magnesium effectively differentiated plasmid-bearing and plasmidless isolates. further, the expression of calcium dependency in plasmid-bearing strains of y. enterocolitica as measured by the average colony diameter was proportional to the calcium concentration of the assay. | 1990 | 2332473 |
| [conjugative r-plasmid resistance of the causative agents of yersinia infection]. | nature, structure, occurrence and drug resistance of 160 strains of y. pseudotuberculosis and 60 strains of y. enterocolitica isolated from various sources within 1986-1988 were studied. in the strains of y. pseudotuberculosis, the cell composition with respect to the requirements in calcium ions as well as the plasmid profiles with determination of the molecular weights of the plasmids in the antibiotic sensitive and resistant pathogens and r(+)-transconjugants were investigated. some molecular ... | 1990 | 2337369 |
| [granulomatous appendicitis: differential "common" appendicitis diagnosis]. | | 1990 | 2342439 |
| [immunostimulating effect of culture filtrates of yersinia enterocolitica]. | application of sterile culturing supernatant of yersinia (y.) enterocolitica (tested serovars were 03 and 08) caused significantly accelerated transplant rejection in mice of various inbreeding strains. action correlated with dosage (r = -0.92). c57b16 mice were tested for their pregnancy rates in response to the same filtrate (serovar 03), with 5.5 live births per mating being recorded from 47 control matings but only 4.4 from 45 experimental matings (alpha less than 0.0025). the mean gestation ... | 1990 | 2344242 |
| the presence of yersinia enterocolitica and other yersinia species on the carcasses of market broilers. | sixty ready-to-cook broiler carcasses obtained from several local supermarkets were tested for the presence of yersinia enterocolitica and other yersinia species. in the present study, the authors used two enrichment broths, yeast-extract/rosebengal-bile oxalate sorbose (yer-bos) and phosphate-buffered saline with a postenrichment koh treatment (pbs-koh), and two plating media, cefsulodin-irgasan-novobiocin (cin) and pectin agar. yersinia organisms were found on 34 of 60 carcasses (56.7%) and y. ... | 1990 | 2345728 |
| [hepato-renal polycystic disease and liver hydatidosis (a rare association)]. | a case is presented of a patient with polycystic kidneys and familial history of this disease who was from a zone of hydatid endemism. the patient had a hepatic mass compatible with hydatid cyst. diagnoses by imaging, like echography and cat, confirmed it, aside from evidencing polycystic liver disease. there was operative and anatomopathologic confirmation of both diseases of the liver. after a review of the bibliography that shows a scant incidence of this concomitance, some remarks are offere ... | 1990 | 2346686 |
| excretion of yersinia spp. associated with consumption of pasteurized milk. | yersinia enterocolitica biotype 1, serotype o.10k was isolated from 19 patients in the paediatric wards of a district general hospital over a period of 3 months. fifteen cases were patients on the medical ward. shortly afterwards, y. enterocolitica biotype 1, serotype o.6, 30 was isolated from a further 17 patients on this ward in 1 month. the same serotypes of y. enterocolitica were isolated from the pasteurized milk supplied to the ward. epidemiological evidence indicated that contaminated pas ... | 1990 | 2347379 |
| presence of specific binding sites for [3h]sarcophytol-a in cultured human skin fibroblasts. | the presence of specific binding sites for [3h]sarcophytol-a in human skin fibroblasts was examined using biochemical and morphological methods. the displacement studies clearly revealed that high (kd = 31.0 nm) and low (kd = 6.05 microm) affinity sites were present in the intact cells. moreover, autoradiographic studies using light microscopy revealed that the specific binding sites may exist in both the cytoplasm and the nuclei. | 1990 | 2347400 |