| frequency of hpv infection and gsh levels in plasma of women with cervical dysplasia. | human papillomavirus (hpv) can be a cause of cervical intraepithelial neoplasia (cin) and cervical cancer. the level of antioxidant compounds in plasma (retinol, alfa-tocoferol, vitamins c and e, glutathione) can modulate the progression of latent hpv infection to subclinical lesions and cin. our studies show that in the cases of hpv infection glutathione (gsh) content decreased, but glutathione disulfide (gssg) levels increased. the quantitative gsh/gssg ratio reflects the above changes of both ... | 1997 | 9174835 |
| the effect of human papillomavirus infection on sperm cell motility. | to investigate the presence of human papillomavirus (hpv) in human sperm cells and to evaluate potential effects of hpv on the sperm functions. | 1997 | 9176459 |
| hpv-16 oncogenes e6 and e7 are mutagenic in normal human oral keratinocytes. | the mutation frequency of ps189 shuttle vector plasmids is higher in human oral keratinocytes (nhok) immortalized with cloned human papillomavirus-16 (hpv-16) genome than in primary normal nhok (nhok). to determine whether oncoproteins e6 and e7 of hpv-16 are responsible for the higher mutation frequency of the plasmids, we measured the mutation frequency in nhok and in nhok expressing the hpv-16 oncogenes (e6, e7, or e6 plus e7). we also measured the mutation frequency in nhok expressing the e6 ... | 1997 | 9178911 |
| clinical and epidemiological correlations between the infection with hpv 16 and hpv 18 and female cervical lesions. | a number of 66 specimens from female cervical lesions were examined for infection with human papillomavirus (hpv) types 6, 11, 16, and 18 by nucleic acid hybridization in dot-blot techniques and 35 sera were tested by the immunodot-blot technique, in order to detect the presence of anti e4 and e7 hpv protein antibodies. the findings were compared with the histologic diagnosis. fifty-six per cent of specimens contained hpv dna sequences. in 47% of specimens from cervical carcinoma, hpv 11 was det ... | 1995 | 9179967 |
| design and methods of a population-based natural history study of cervical neoplasia in a rural province of costa rica: the guanacaste project. | this paper reports on the enrollment phase of a population-based natural history study of cervical neoplasia in guanacaste, a rural province of costa rica with consistently high rates of invasive cervical cancer. the main goals of the study are to investigate the role of human papillomavirus (hpv) infection and its co-factors in the etiology of high-grade cervical neoplasia, and to evaluate new cervical cancer screening technologies. to begin, a random sample of censal segments was selected and ... | 1997 | 9180057 |
| p53 mutations in relation to human papillomavirus type 16 infection in squamous cell carcinomas of the head and neck. | the relationship between human papillomavirus (hpv) type 16 infection and p53 gene mutations was investigated in squamous cell carcinomas of the head and neck (scchn). hpv was detected by general primer-mediated and type-specific pcr. alterations in the p53 gene were investigated using single-strand conformation polymorphism and sequence analysis in 27 scchn, of which 12 were hpv 16-positive and 15 were hpv-negative. mutations were detected in 2/12 (16.7%) hpv 16-positive and 7/15 (46.7%) hpv-ne ... | 1997 | 9180148 |
| human papillomavirus variants: implications for natural history studies and vaccine development efforts. | | 1997 | 9182967 |
| probing for cerebrospinal fluid antibody specificities by a panel of random peptide libraries. | in a state of health the central nervous system is nearly devoid of macrophages, t and b cells. however, such cells are required to initiate the inflammation associated with multiple sclerosis (ms). an important issue in understanding the pathogenesis of such conditions is to fully define the t and b cell specificities. using random peptide libraries, we have analysed the cerebrospinal fluid antibody specificities within one oligoclonal "band" from a patient with ms. the selected peptides from a ... | 1997 | 9189009 |
| presence of human papillomavirus in esophageal squamous cell carcinomas of hong kong chinese and its relationship with p53 gene mutation. | there is no scientific study that has investigated the association between human papilloma virus (hpv) and p53 mutation in hong kong chinese patients with esophageal cancers. the aim of this survey is to evaluate in details the prevalence and relationship of hpv and p53 mutation in these patients with esophageal squamous cell carcinomas. fresh tissues from the resected specimens of 70 chinese patients (59 men, 11 women) with primary esophageal squamous cell carcinomas (20 well-differentiated, 36 ... | 1997 | 9190999 |
| interlaboratory agreement among results of human papillomavirus type 16 enzyme-linked immunosorbent assays. | serological assays for measuring antibodies to human papillomavirus type 16 (hpv-16) virus-like particles (vlps) have become important epidemiologic tools in recent years. however, the interlaboratory replicability of these assays has not been assessed. in this investigation, three laboratories tested a panel of specimens obtained from two different groups: 265 subjects in a vulvar cancer case-control study and 107 healthy volunteer blood donors. each laboratory used an enzyme-linked immunosorbe ... | 1997 | 9196186 |
| human papilloma virus 16-18 infection and cervical cancer in mexico: a case-control study. | cervical cancer (cc) is one of the principal public health problems in mexico. the national mortality rate due to cc was estimated at 21.8 per 100,000 among women over 15 years old during 1994. despite this high incidence little is known in mexico about the risk factors for cc. the objectives of the study were to evaluate the association between cc and hpv types 16 and 18 in women living in mexico city. from august, 1990 to december, 1992, a case-control study was carried out in the metropolitan ... | 1997 | 9204620 |
| two cytodifferentiation agent-induced pathways, differentiation and apoptosis, are distinguished by the expression of human papillomavirus 16 e7 in human bladder carcinoma cells. | many transformed cells have been found to lose the capacity to proliferate and undergo differentiation following exposure to hybrid polar agents. this study investigates the mechanism by which hexamethylene bisacetamide (hmba) suppresses the proliferation of the human bladder carcinoma line, t24. we found that following a 24-h exposure to hmba, t24 proliferation was inhibited, and cells arrested in g1 phase and underwent morphological maturation. hmba-induced cessation of proliferation was media ... | 1997 | 9205091 |
| idiopathic cd4+ t-lymphocytopenia and recurrent vulvar intraepithelial neoplasia. | cd4+ t-lymphocytopenia immunodeficiency without human immunodeficiency virus (hiv) infection has been reported recently. the association between immunodeficiency and anogenital neoplasia secondary to human papillomavirus infections is well documented. | 1994 | 9205460 |
| dna analysis indicates patient-specific human papillomavirus type 16 strains in bowen's disease on fingers and in archival samples from genital dysplasia. | human papillomavirus (hpv) type 16 is casually involved in the pathogenesis of anogenital cancer and has also been demonstrated in some patients with bowen's disease (bd) on the fingers. from two women with hpv 16 in bd on the fingers, and in archival samples from genital dysplasia, collected as long as 26 years ago, the non-coding region of the virus was amplified by the polymerase chain reaction and sequenced. the hpv 16 dna sequences found in the finger lesions and in the genital archival sam ... | 1997 | 9205498 |
| [study on the interrelationship between human papilloma virus infection and langerhans cell in carcinogenesis of esophagus]. | in order to find out the interrelationship between human papilloma virus (hpv) infection and langerhans cells (lcs) during the development of esophageal carcinoma, and the mechanism of carcinogenesis during hpv infection, digoxigenine labelled hpv dna probes of hpv 6b/11, hpv 16/18 with in situ hybridization, and anti-s-100 protein antibody with immunohistochemical lsab-assays were used respectively in order to investigate hpv infection and the distribution of lcs in 40 cases of esophageal squam ... | 1996 | 9206210 |
| lymphoepithelioma-like carcinoma of the uterine cervix: association with epstein-barr virus and human papillomavirus. | the presence of epstein-barr virus (ebv) has not been documented in previous reports of lymphoepithelioma-like carcinoma (lelc) of the uterine cervix by either polymerase chain reaction or in situ hybridization, and the histogenesis of the tumor remains unknown. additionally, a relationship between human papillomavirus (hpv) and cervical lelc also has not been reported. | 1997 | 9210713 |
| ha-ras oncogene-induced transcription of human papillomavirus type 18 e6 and e7 oncogenes. | human papillomavirus (hpv) dna sequences are found in most carcinomas originating from the uterine cervix. hpv e6 and e7 oncogenes have been shown to cooperate with ras oncogenes to fully transform human epithelial cells. we investigated the effect of the ha-ras oncogene on the transcriptional activity of hpv-18 and found that it induced the transcriptional activity of the viral promoter, whereas the normal gene had only a minimal effect. however, transfection of the normal ha-ras gene and simul ... | 1997 | 9210955 |
| complex genomic rearrangement within the 12q15 multiple aberration region induced by integrated human papillomavirus 18 in a cervical carcinoma cell line. | human papillomavirus (hpv) dna is integrated into the host genome in cervical cancer. the cervical carcinoma cell line sw756 has integrated hpv-18 dna in chromosome region 12q15, in the papillomavirus-associated locus-2 (pal2). by polymerase chain reaction and hybridization of an arrayed cosmid library with oligonucleotides from the rearranged allele, we determined the pre-integration germline structure of the region. pal2 was located approximately 10 kb from sequence-tagged site marker u27131, ... | 1997 | 9210958 |
| androgen responsive adult human prostatic epithelial cell lines immortalized by human papillomavirus 18. | prostate cancer and benign tumors of the prostate are the two most common neoplastic diseases in men in the united states, however, research on their causes and treatment has been slow because of the difficulty in obtaining fresh samples of human tissue and a lack of well characterized cell lines which exhibit growth and differentiation characteristics of normal prostatic epithelium. non-neoplastic adult human prostatic epithelial cells from a white male donor were immortalized with human papill ... | 1997 | 9214605 |
| acinar differentiation by non-malignant immortalized human prostatic epithelial cells and its loss by malignant cells. | invasive prostatic carcinomas and prostatic intraepithelial neoplasia (pin) are characterized by a loss of normal cell organization, cell polarity, and cell:cell and cell:basement membrane adhesion. the objective of this study was to establish in vitro three-dimensional (3-d) cell models which can be used to investigate mechanisms involved in acinar morphogenesis and differentiation in normal prostatic epithelium and their abnormalities in cancer cells. the process of acinar morphogenesis, inclu ... | 1997 | 9214606 |
| detection of human papillomavirus infection in squamous tumours of the conjunctiva and lacrimal sac by immunohistochemistry, in situ hybridisation, and polymerase chain reaction. | squamous tumours of the ocular surface, including the lacrimal pathway, range from benign lesions to invasive carcinomas. some of these tumours are associated with human papillomavirus (hpv) infection, with the types of hpv differing among papillomas and dysplastic or malignant lesions. | 1997 | 9215061 |
| seropositivity against hpv 16 capsids: a better marker of past sexual behaviour than presence of hpv dna. | to assess if seropositivity to human papillomavirus type 16 capsids is a better marker of sexual history than the presence of hpv dna. | 1997 | 9215097 |
| human papillomaviruses are not associated with renal carcinoma. | in an attempt to better understand the troubling issue of renal human papillomavirus (hpv) infection, fresh tumour specimens obtained by surgical extirpation from tumours were compared for the presence of hpv. all of the renal carcinoma samples were examined by pcr using two sets of consensus primers and the specific primer pairs for hpv 16, 18, and 33. none of the 28 carcinomas and 17 corresponding normal tissues were found positive for hpv dna in any of the applied analyses. our results sugges ... | 1997 | 9216686 |
| p53 protein overexpression in laryngeal squamous cell papillomas. | we retrospectively investigated p53 protein immunoreactivity in 103 laryngeal squamous cell papillomas (lp) previously revealed to be human papillomavirus type 6 or 11 positive by in situ hybridization and/or the polymerase chain reaction. 21 lp failed to show any detectable level of p53 protein reactivity. in 45 cases only occasional strongly positive cells were observed in almost the whole thickness of the epithelium. in 26 lp, p53 protein immunoreactivity was found to be almost exclusively re ... | 1997 | 9216688 |
| coinfection of hpv-11 and hpv-16 in a case of laryngeal squamous papillomas with severe dysplasia. | human papillomavirus (hpv) types 6 and 11 have been associated with benign laryngeal papilloma, while hpv-16 is occasionally associated with laryngeal carcinoma. in this study, a case of laryngeal squamous papillomas with severe dysplasia was evaluated for the presence of hpv infection. the biopsy specimens were taken from a 58-year-old female patient at two different time points 3 months apart. architecturally, the tumor showed papillary configuration reminiscent of squamous papilloma. cytologi ... | 1997 | 9217136 |
| perspectives on human papillomavirus infection. | | 1997 | 9217655 |
| human papillomavirus and human disease. | human papillomaviruses (hpvs) are associated with a spectrum of different diseases in humans, including common warts and genital warts. of more serious concern is the connection between certain hpv types and some malignancies, particularly cervical and anal cancer. dna from hpv-16 and hpv-18, two types frequently found in cervical cancer tissue, can immortalize cells in laboratory cultures, unlike dna from hpv types associated with benign genital lesions. although it is unclear how high-risk hpv ... | 1997 | 9217657 |
| clinical presentation and natural course of anogenital warts. | the natural history of human papillomavirus (hpv) genital tract lesions is complex, partly because infection can appear in several forms and often follows a fluctuating course. the primary mode of transmission of genital strains of hpv is through sexual contact. most clinically apparent genital warts are caused by hpv type 6 or 11. the main manifestations of anogenital warts are cauliflower-like condylomata acuminata that usually involve moist surfaces; keratotic and smooth papular warts, usuall ... | 1997 | 9217658 |
| counseling patients with genital warts. | counseling patients about any sexually transmitted disease (std) is difficult, for both the physician and the patient, but a diagnosis of genital warts presents particular challenges. for many patients, being told that they have any std comes as a shock. although fear is a common reaction, the relationship between human papillomavirus (hpv) and cancer has made the presence of genital warts especially frightening. this fear is heightened by the fact that treatment will not eradicate the underlyin ... | 1997 | 9217661 |
| mutational analyses of differentiation-dependent human papillomavirus type 18 enhancer elements in epithelial raft cultures of neonatal foreskin keratinocytes. | human papillomaviruses (hpvs) reproduce only in differentiated squamous epithelia. viral transcription is rather restricted in basal strata but increases dramatically in the spinous cells. inopportune viral oncoprotein expression in the basal reserve cells can lead to dysplasias and carcinomas. until now all studies to identify transcription factor binding sites within the upstream regulatory region (urr) that controls the expression of the oncogene have been conducted in proliferating cell cult ... | 1997 | 9218869 |
| two classes of human papillomavirus type 16 e1 mutants suggest pleiotropic conformational constraints affecting e1 multimerization, e2 interaction, and interaction with cellular proteins. | random mutagenesis of human papillomavirus type 16 (hpv16) e1 was used to generate e1 missense mutants defective for interaction with either hubc9 or 16e1-bp, two cdnas encoding proteins that have been identified by their ability to interact with hpv16 e1 in two-hybrid assays. hubc9, the human counterpart of saccharomyces cerevisiae ubc9, is a ubiquitin-conjugating enzyme known to be involved in cell cycle progression. 16e1-bp encodes a protein of no known function but does contain an atpase sig ... | 1997 | 9223484 |
| characterization of a human papillomavirus type 16 variant-dependent neutralizing epitope. | we have determined that three type-specific and conformationally dependent monoclonal antibodies, h16.e70, h16.u4, and h16.v5, neutralize pseudotype human papillomavirus type 16 (hpv16) virions in vitro. h16.u4 and h16.v5 neutralized pseudotype virions derived from the german hpv16 variant 114k and the zairian variant z-1194 with equal efficiency. in contrast, neutralization of z-1194 pseudotype virions by h16.e70 was two orders of magnitude weaker than neutralization of 114k pseudotype virions. ... | 1997 | 9223527 |
| prevalence of human papilloma virus 16 or 18 in cervical cancer in hualien, eastern taiwan. | in order to determine the association of human papilloma virus (hpv) with cervical cancer of patients in the hualien area, we analyzed 40 cervical cancer specimens using polymerase chain reaction (pcr) to detect the presence of hpv type 16 (hpv-16) and type 18 (hpv-18) genomes. the results showed that at least 70% (28/40) of the specimens had hpv dna. of the 37 squamous cell carcinomas of the cervix, hpv-16 was present in 25 cases (68%) and hpv-18 in 2 (5.4%). hpv-16 dna was detected in one of 2 ... | 1997 | 9226973 |
| clinical and molecular aspects of squamous cell carcinoma of the head and neck in the nonsmoker and nondrinker. | most molecular analyses of head and neck squamous cell carcinoma involve patients who have used tobacco and alcohol. the pathways involved in tumorigenesis in patients lacking these lifestyle risks may be quite different. research involving rigorous epidemiologic and molecular methods is needed to identify the unique spectra of genetic alterations in subsets of the population. this review considers recent information on the accumulation of genetic changes in the typical head and neck squamous ce ... | 1997 | 9229148 |
| infiltration of cervical cancer tissue with human papillomavirus-specific cytotoxic t-lymphocytes. | ctls specific for high-risk human papillomaviruses (hpvs) have recently been found in the peripheral blood of cervical cancer patients. although cell-mediated immunity is thought to be important in the control of hpv infection, the functional relevance and site of activation of hpv-specific ctls are unclear. we identified hla-a*0201-restricted hpv-16 e7 peptide-specific ctls in the peripheral blood (four of five patients), draining lymph nodes (three of four patients) and tumors (one of three pa ... | 1997 | 9230206 |
| alternatively spliced hpv-18 e6* protein inhibits e6 mediated degradation of p53 and suppresses transformed cell growth. | the e6 proteins originating from the tumour-associated human papillomavirus (hpv) types 16 and 18 have been shown to bind to and target the tumour suppressor protein, p53, for ubiquitin-mediated degradation. however, in cell lines derived from cervical neoplasias, the predominant early region transcripts are spliced and encode truncated forms of e6, termed e6*. we report here that hpv-18 e6* protein will interact both with the full-length e6 proteins from hpv-16 and hpv-18 and also with e6-ap, a ... | 1997 | 9233760 |
| p53-mediated accumulation of hypophosphorylated prb after the g1 restriction point fails to halt cell cycle progression. | this study analyses whether the inability of p53 to induce g1 arrest after the restriction point relates to an inability to modulate prb phosphorylation. transient p53 overexpression in normal human diploid fibroblasts and p53-deficient cancer cells led to increased levels of the cyclin-dependent kinase inhibitor p21 cip1/waf1/sdi1 and an accumulation of hypophosphorylated prb in cells growing asynchronously and in cells synchronized in late g1 or m. similarly, gamma-irradiation of asynchronous, ... | 1997 | 9233768 |
| pregnancy and cervical infection with human papillomaviruses. | this investigation was undertaken to assess whether pregnancy represents a risk factor for human papillomavirus (hpv) infection which is independent of age and other known hpv risk factors. | 1996 | 9236309 |
| prevalence of penile human papillomavirus dna in husbands of women with and without cervical neoplasia: a study in spain and colombia. | to investigate the role of men in cervical cancer, 816 husbands of women enrolled in four case-control studies of cervical neoplasia in populations at high (colombia) and low (spain) risk for cervical cancer were interviewed. exfoliated cells from the penis were obtained and analyzed by polymerase chain reaction for the presence of human papillomavirus (hpv) dna. penile hpv dna prevalences were higher in husbands of women with cervical neoplasia than in husbands of controls. husbands of controls ... | 1997 | 9237700 |
| papillary (pebbled) masses of the oral mucosa: more than simple papillomas. | soft tissue masses are found in almost 4% of adult dental patients, and most of these will never be biopsied. clinical evaluation, therefore, is of paramount importance. oral mucosal masses with irregular or nodular surface alterations are of special concern. these alterations may appear to be papillomas, but there are several type of papillomas, and among the lesions which present with a papilloma-like appearance are diverse malignancies, contagious infections, sexually transmitted diseases, va ... | 1996 | 9242122 |
| deletions of chromosome 3p are frequent and early events in the pathogenesis of uterine cervical carcinoma. | to study the molecular abnormalities involved in the multistage development of cervical carcinoma (cc), we investigated the presence of oncogenic human papillomavirus (hpv) sequences, loss of heterozygosity (loh), and microsatellite alterations at several genes/loci at 3p (3p14.2 at the fhit gene, 3p14.3-21.1, 3p21, and 3p22-24.2), 9p21, rb and p53, and p53 gene point mutations in precisely microdissected archival tissues from 20 ccs and their accompanying precursor lesions (cervical intraepithe ... | 1997 | 9242443 |
| [follow-up of high risk, human papillomavirus (hpv)-positive patients with cancer of the uterine cervix]. | the study population consisted of 30 cervical cancer patients stage i.a-ii.b. (figo) stages operated on according to the wertheim technique. a parallel histological evaluation and hpv status determination were carried out on biopsies from the primary tumors and on the regional lymph nodes. a general primer mediated polymerase chain reaction (pcr) was performed at first and the samples not amplified were examined by type-specific primers. all except one primary tumors contained dna-sequences char ... | 1997 | 9244858 |
| cytomorphologic evidence of human papillomavirus infection in smears from the irradiated uterine cervix. | to report the cytomorphologic evidence of human papillomavirus (hpv) infection in cervical smears from women treated with radiotherapy for carcinoma of the uterine cervix. | 1997 | 9250302 |
| human papillomavirus antibody responses among patients with incident cervical carcinoma. | the human papillomavirus (hpv) is recognized as a major cause of cervical cancer precursor lesions. hpv serology is a key method in the continuing elucidation of the importance of hpv exposure for cancer development and in predicting hpv-associated diseases. to extend previous hpv serological studies on cervical cancer, serum samples from a consecutive series of 216 women with incident untreated cervical carcinoma and 243 age- and sex-matched healthy blood donors were evaluated for the presence ... | 1997 | 9260694 |
| association between human papillomavirus type 18 variants and histopathology of cervical cancer. | | 1997 | 9274919 |
| different susceptibility of cervical keratinocytes containing human papillomavirus to cell-mediated cytotoxicity. | to detect the factors responsible for the susceptibility of cervical keratinocytes infected with human papillomavirus (hpv) to non-specific lysis mediated by natural killer (nk) and lymphokine activated killer (lak) cells. | 1996 | 9275369 |
| [clinicopathological features of human papilloma virus associated squamous cell carcinomas of the vulva]. | to investigate the relationship between human papilloma virus (hpv) status and clinicopathological features of vulva carcinoma and its clinical significance. | 1996 | 9275429 |
| [role of pcr and dot bolt hybridization in the detection of human papillomavirus of the penile cancer]. | human papillomavirus (hpv), a oncogenicpotential, may be carcinogenic effectors in variety of genital tract malignancies. we examined the association of human papillomavirus type 16 and 18 in 28 penile cancers by the highly sensitive polymerase chain reaction and dot bolt hybridization. human papillomavirus type 16 was found in 14 of 28 penile cancers (50%), whereas type 18 was identified in 3 of 28 penile cancers (10.6%). our results suggest humanpapillomavirus type 16 may play a role in the de ... | 1996 | 9275681 |
| cancer-associated human papillomavirus types are selectively increased in the cervix of women in the first trimester of pregnancy. | | 1997 | 9279838 |
| [detection of the human papillomavirus in hyperplastic and cancerous prostatic tissue with pcr]. | human papillomavirus (hpv) infections are frequently associated with benign, pre-malignant and malignant lesions of the female genital region and male anogenital tract. recently several studies have been performed to evaluate the presence of hpv in the adenomatous and carcinomatous prostatic tissue for the epidemiological implications due to the sexual transmission, and for the possible etiologic role in the development of prostatic carcinoma, first cause of death from neoplasia in men over 60 y ... | 1997 | 9281080 |
| the human papillomavirus e7 oncoprotein can uncouple cellular differentiation and proliferation in human keratinocytes by abrogating p21cip1-mediated inhibition of cdk2. | the high risk human papillomaviruses (hpvs) are associated etiologically with the majority of human cervical carcinomas. these hpvs encode two viral oncoproteins, e6 and e7, which are expressed consistently in cervical cancers. the function of these viral oncoproteins during a productive infection is to ensure viral replication in cells that have normally withdrawn from the cell division cycle and are committed to terminal differentiation. expression of the e7 oncoprotein has been shown to lead ... | 1997 | 9284049 |
| mechanism of hpv e6 proteins in cellular transformation. | the e6 protein is a major transforming protein of many types of papillomaviruses. mechanistically, the best characterized e6 proteins are those of the high-risk genital hpvs (e.g. hpv-16 and 18 e6), which function, at least in part, by inactivating the p53 tumor suppressor protein. biochemical studies have shown that this occurs by targeted degradation of p53, dependent on the e6-ap ubiquitin-protein ligase. the model that has emerged from e6/e6-ap-dependent p53 degradation has provided insight ... | 1996 | 9284524 |
| interactions of the human papillomavirus e7 protein with cell cycle regulators. | human papillomavirus (hpvs) critically depend on the cellular machinery for the replication of their genome. viral replication is restricted to the differentiated strata of the skin that are normally growth arrested. hence, the hpvs have developed strategies to subvert cellular growth regulatory pathways and are able to uncouple cellular proliferation and differentiation. the hpv e7 protein can overcome the activity of some cyclin-dependent kinase inhibitors, associate with cyclin/cyclin depende ... | 1996 | 9284525 |
| hla control in the progression of human papillomavirus infections. | cellular immunity is likely to be of major importance for the clearance of inapparent or overt infections caused by human papillomaviruses (hpvs). the highly polymorphic class i or class ii human leukocyte antigen (hla) molecules present hpv-derived peptides to cytotoxic (cd8+) or helper (cd4+) t lymphocytes bearing specific receptors and condition the immune responsiveness to hpv infections. recent data point to a role of an altered expression of hla molecules in the persistence of hpv-induced ... | 1996 | 9284528 |
| generation of in vitro autologous human cytotoxic t-cell response to e7 and her-2/neu oncogene products using ex-vivo peptide loaded dendritic cells. | | 1997 | 9286422 |
| [detection of human papillomavirus gene sequences in laryngeal tumors and premalignant changes by polymerase chain reaction]. | human papillomavirus gene sequences have been detected in a number of malignant and benign tumours. non-oncogenic types 6 and 11 are etiological factors of benign mucosal tumours. types 16 and 18 can be detected in malignancies most often but their role in the etiopathogenesis of cancers is still unclear. in our study we examined formalin-fixed and paraffin-embedded archive laryngeal tissues containing squamous cell carcinoma, papilloma and precancerous lesions for the presence of human papillom ... | 1997 | 9289687 |
| a monoclonal antibody against intact human papillomavirus type 16 capsids blocks the serological reactivity of most human sera. | a type-specific and neutralizing mouse mab (v5) against human papillomavirus (hpv) type 16 capsids was found to block the serological reactivity of human sera with the corresponding capsids. out of 352 human serum samples tested for the presence of igg against hpv-16, more than 75% of reactive sera were completely blocked by the v5 antibody. type-specific mabs against hpv-6, -18 and -33 were also found to block serological reactivity with capsids of the corresponding hpv types for the majority o ... | 1997 | 9292008 |
| type-specific prevalence of genital human papillomaviruses in benign, premalignant, and malignant biopsies in patients from greece. | more than 30 different human papillomavirus (hpv) types infect the anogenital mucosa and are responsible for a variety of benign, premalignant, and malignant lesions including cervical cancer. the goal of this study was to determine the distribution of individual hpv types in various grads of cervical precancerous lesions and cervical carcinoma in patients from greece. | 1997 | 9293610 |
| lymphoproliferative responses to human papillomavirus (hpv) type 16 proteins e6 and e7: outcome of hpv infection and associated neoplasia. | infection with human papillomavirus (hpv) type 16 (hpv16) is a major cause of high-grade cervical intraepithelial neoplasia (cin). experiments were planned to evaluate the role of cell-mediated immunity (e.g., lymphocyte proliferation) against hpv in the natural history of hpv-associated neoplasia and to identify antigenic sequences of the hpv16 proteins e6 and e7 against which an immune response may confer protection. | 1997 | 9293919 |
| genetic alterations in patients with esophageal cancer with short- and long-term survival rates after curative esophagectomy. | the objective of this study was to ascertain the exact relation between specific oncogenes and long- and short-term survival rates in patients with esophageal cancer. | 1997 | 9296509 |
| incidence of human papillomavirus 6, 11, 16, 18 and 33 in normal oral mucosa of a greek population. | the polymerase chain reaction (pcr) was applied for the detection of human papillomavirus (hpv) infection in samples obtained from the clinically normal mucosa of the oral cavity of 169 asymptomatic subjects in northern greece. of the subjects, 9.5% were found to be infected with hpv. typing of hpv by southern blot hybridization revealed that 2.4%, 0%, 0%, 4.1%, 0.6% of the subjects were infected with hpv 16, 18, 33, 6 and 11, respectively. | 1997 | 9298359 |
| carcinomas occurring in papillomas of the nasal septum associated with human papilloma virus (hpv). | carcinomas arising in pre-existing sinonasal papillomas of the nasal septum are rare. to our knowledge only one case has been reported. we report two cases of carcinomas occurring in septal papillomas. in the first case a carcinoma developed in an exophytic papilloma 16 years after the first operation for a papilloma. in the second case a carcinoma was present at the first presentation within an inverted papilloma, and a metastasis had also developed. in the first case hpv type 6/11 was demonstr ... | 1997 | 9299655 |
| anal human papillomavirus and anal cancer. | | 1997 | 9301544 |
| [the detection of human papillomavirus in papillomas of the larynx and tonsils through immunohistochemistry and dna in situ hybridization]. | presenting a study about human papillomavirus (hpv) determination by immunohistochemistry and "in situ" hybridization, in 25 samples of squamous cell papillomata (15 tonsilar and 10 laryngeal lesions). five cases resulted positive for hpv: 2 of them for immunohistochemical probes, other 2 for "in situ" hybridization and only 1 cas showed its positivity for both techniques. all these samples belonging to laryngeal cases. no significant differences were found in antecedents, clinical and histologi ... | 1997 | 9304351 |
| analysis of hpv16, 18, 31, and 35 dna in pre-invasive and invasive lesions of the uterine cervix. | to analyse the physical state of different human papillomavirus (hpv) dnas in 55 intraepithelial and invasive hpv associated cervical neoplasms. | 1997 | 9306943 |
| prevalence of epstein-barr virus in oral squamous cell carcinomas, premalignant lesions and normal mucosa--a study using the polymerase chain reaction. | epstein-barr virus (ebv) prevalence was assessed in 12 clinically normal oral mucosas, nine premalignant lesions, 36 oral squamous cell carcinomas (osccs) and a human papillomavirus (hpv) 16 positive cell line, derived from an oscc studied. the polymerase chain reaction (pcr) with two pairs of primers with different sensitivities was used. with primers specific for the bamhiw repeat, ebv was found in 100% of osccs, in 77.8% of premalignant lesions and in 8.3% of clinically normal oral mucosas (p ... | 1997 | 9307727 |
| human papillomavirus and other risk factors for cervical cancer. | cancer of the cervix is the second most common cancer in women after cancer of the breast, and the commonest in developing countries. in the last decade the etiology of cervical cancer has been unequivocally established--over 90% of the cases in all countries are related to infections to some strains of human papillomavirus (hpv). these infections are common in young age groups and resolve spontaneously in most instances. the typical hpv deoxyribonucleic acid (dna) prevalence after the third dec ... | 1997 | 9309247 |
| is human papillomavirus an infectious cause of non-cervical anogenital tract cancers? | | 1997 | 9310555 |
| prospective seroepidemiological study of role of human papillomavirus in non-cervical anogenital cancers. | to evaluate the association between infection with the major oncogenic types of human papillomavirus and the risk of developing non-cervical anogenital cancers in a cohort followed up prospectively. | 1997 | 9310566 |
| synthesis of infectious human papillomavirus type 18 in differentiating epithelium transfected with viral dna. | the lack of a permissive system for the propagation of viral stocks containing abundant human papillomavirus (hpv) particles has hindered the study of infectivity and the early stages of hpv replication. the organotypic (raft) culture system has permitted the study of a number of the differentiation-specific aspects of hpv, including amplification of viral dna, expression of late genes, and viral morphogenesis. however, these investigations have been limited to a single virus type, namely, hpv t ... | 1997 | 9311816 |
| conserved interaction of the papillomavirus e2 transcriptional activator proteins with human and yeast tfiib proteins. | papillomavirus early gene expression is regulated by the virus gene-encoded e2 proteins. the best-characterized e2 protein, encoded by bovine papillomavirus type 1 (bpv-1), has been shown to interact with basal transcription factor iib (tfiib) and the tata binding protein basal transcription factor (n. m. rank and p. f. lambert, j. virol. 69:6323-6334, 1995). we demonstrate that the potent e2 transcriptional activator protein encoded by a gene of human pv type 16 also interacts with tfiib in vit ... | 1997 | 9311902 |
| p53 protein overexpression is common and independent of human papillomavirus infection in squamous cell carcinoma of the vulva. | human papillomavirus (hpv) infection and p53 abnormalities might both play a role in the carcinogenesis of subtypes of squamous cell carcinoma of the vulva. in this study, the authors investigated the prevalence of and the relationship between p53 overexpression and hpv infection in patients with vulvar carcinoma. | 1997 | 9317172 |
| selection of human cervical epithelial cells that possess reduced apoptotic potential to low-oxygen conditions. | since human papillomavirus (hpv) infection is strongly associated with cervical neoplasia and tumor hypoxia has prognostic significance in human cervical carcinomas, we examined the relationship between hypoxia and apoptosis in human cervical epithelial cells expressing high-risk hpv type 16 oncoproteins. in vitro, hypoxia stimulated both p53 induction and apoptosis in primary cervical epithelial cells infected with the hpv e6 and e7 genes but not in cervical fibroblasts infected with e6 and e7. ... | 1997 | 9331075 |
| skin autografts in epidermodysplasia verruciformis: human papillomavirus-associated cutaneous changes need over 20 years for malignant conversion. | epidermodysplasia verruciformis (ev) is regarded as a model for cutaneous oncogenesis associated with specific human papillomaviruses (hpvs). because genital hpv-associated carcinogenesis is a very long-lasting process requiring 20-30 years and epidemiological studies of this type for hpv-associated skin cancers are impossible in such a rare disease as ev, we observed for up to 20 years ev patients having surgery for carcinomas with consecutive autografts from uninvolved and non-sun-exposed skin ... | 1997 | 9331078 |
| immune activation in cervical neoplasia: cross-sectional association between plasma soluble interleukin 2 receptor levels and disease. | in a previous study (tsukui et al., cancer res., 56: 3967-3974, 1996), we observed an inverse association between degree of cervical neoplasia and interleukin (il) 2 production by peripheral blood mononuclear cells in response to human papillomavirus (hpv) 16 e6 and e7 peptides in vitro. this suggested that a th1-mediated cellular immune response might be important in host immunological control of hpv infection and that a lack of such a response might predispose to progression of cervical diseas ... | 1997 | 9332763 |
| serum levels of soluble tumor-necrosis-factor receptors in patients with benign and malignant hpv-associated anogenital lesions. | the levels of type-i and type-ii soluble tnf-alpha receptors (stnf-rs) were evaluated in sera from patients with various human-papillomavirus-(hpv)-associated benign and malignant anogenital lesions using specific enzyme-linked immunobiological assays. in patients with benign hpv6/11-associated condylomata acuminata, the levels of stnf-ri were significantly increased, while stnf-rii were in normal range. both types of stnf-rs were in normal range in patients with benign hpv16-associated grade-i/ ... | 1997 | 9334803 |
| common association of hpv 2 with anogenital warts in prepubertal children. | anogenital (ag) warts in 31 prepubertal children were hpv typed by nonisotopic in situ hybridization (nish) using digoxigenin-labeled probes for human papilloma virus (hpv) types 1-5, 6, 11, 16, 18, 31, and 33. mode of transmission was determined from historical, clinical, and laboratory data independent of hpv typing. hpv 2 was detected most commonly (13/31 warts) followed by hpv 6 (7/31), hpv 11 (5/31), and hpv 16 (1/31). although not reaching statistical significance, our results suggested th ... | 1997 | 9336800 |
| cervical cancer and human papillomavirus: epidemiological evidence and perspectives for prevention. | cervical cancer is a major public health problem, as it is the second most common cancer in women world-wide after breast cancer. about 80% of the half a million cases estimated to occur annually in the world, occur in developing countries. the epidemiological evidence linking human papillomavirus (hpv) to cervical cancer is reviewed. it is concluded that over 90% of cervical cancers can be attributed to certain hpv types. hpv 16 accounts for the highest proportion (50%) followed by hpv 18 (12%) ... | 1997 | 9337560 |
| in situ hybridization and its diagnostic applications in pathology. | in situ hybridization (ish) is a technique by which specific nucleotide sequences are identified in cells or tissue sections. these may be endogenous, bacterial or viral, dna or rna. on the basis of research applications, the technique is now being translated into diagnostic practice, mainly in the areas of gene expression, infection and interphase cytogenetics. diagnostic applications are most often based on short nucleotide sequences (oligomers) labelled with non-isotopic reporter molecules, a ... | 1997 | 9349226 |
| cytokeratin patterns of expression in human epithelial cell lines correlate with transcriptional activity of the human papillomavirus type 16 upstream regulatory region. | a comparison of the cat reporter activity of a plasmid which contains the 232 bp epithelial specific enhancer alone with that of plasmids which contain additional sequences from the human papillomavirus type 16 (hpv-16) upstream regulatory region (urr) revealed two markedly different patterns, in an analysis of six human epithelial cell lines. in hela, c33a and siha, the cat reporter activities of all the constructs were comparable. in contrast, in caski, hk2be6-e7 and hacat we detected very low ... | 1997 | 9349481 |
| pathogenesis of human papillomavirus infection of the genitals. | | 1997 | 9350117 |
| human papillomavirus-specific cytotoxic t lymphocytes in patients with cervical intraepithelial neoplasia grade iii. | human papillomaviruses (hpvs), especially types 16 and 18, are strongly associated with the development of cervical intraepithelial neoplasia (cin) and invasive carcinoma. the hpv e6 and e7 proteins are expressed constitutively in the majority of cin lesions and carcinomas. therefore, they are targets for the immune response against hpv and candidates for active immunotherapy. we have previously detected hpv-specific ctls from the peripheral blood mononuclear cells from a cervical cancer patient ... | 1997 | 9354449 |
| sexually transmitted infection as a cause of anal cancer. | the incidence of anal cancer has increased in recent decades, particularly among women. to identify underlying risk factors, we conducted a population-based case-control study in denmark and sweden. | 1997 | 9358129 |
| carcinoma of the lung in okinawa, japan: with special reference to squamous cell carcinoma and squamous metaplasia. | in okinawa, a subtropical island in southern japan, squamous cell carcinoma (scc), especially the well-differentiated form, is prevalent, while this form is relatively rare in both the mainland and other countries (e.g. united states of america). more patients with scc from okinawa, moreover, were positive for human papillomavirus (hpv) dna by polymerase chain reaction (pcr) (79%), and harbored hpv types 6, 16 and 18, in combination. on the other hand, less than 30% of the mainland patients were ... | 1997 | 9361099 |
| human papillomavirus infection in squamous cell carcinoma of the vulva, in various synchronous epithelial changes and in normal vulvar skin. | to investigate the prevalence of human papillomavirus (hpv) infection in various vulvar lesions. | 1997 | 9367704 |
| genetically modified bone marrow-derived dendritic cells expressing tumor-associated viral or "self" antigens induce antitumor immunity in vivo. | the clinical application of synthetic tumor peptide-based vaccines is currently limited to patients with specified major histocompatibility complex (mhc) class i alleles. such logistic limitations may be overcome using tumor gene-based approaches. here we describe the effective generation of dendritic cells (dc) expressing tumor peptide-mhc complexes as a result of particle-mediated transfer of genes encoding tumor-associated antigens (taa). bone marrow-derived dc were transfected with plasmid d ... | 1997 | 9368629 |
| molecular analysis of the interaction between hpv type 16 e6 and human e6-associated protein. | the complex formed between the human papillomavirus type 16 e6 protein and human e6-associated protein, which combine to ubiquitylate and degrade p53, has been studied by chemical crosslinking. analysis of the interactions of proteins purified from escherichia coli as well as proteins expressed in insect cells indicates that, while e6 has the capacity to form dimers, e6 and e6-associated protein interact as two monomers to form a heterologous dimer. | 1997 | 9369221 |
| immunotherapy of cancer by peptide-based vaccines for the induction of tumor-specific t cell immunity. | recent progress in defining the molecular nature of antigens and in finding ways to manipulate t cell-mediated immune responses may provide new modalities for cancer treatment. in this report, we review preclinical studies as well as the first clinical trials with vaccination strategies aiming at the induction of anti-tumor immunity. in particular, we focus on the development of a vaccine against human papillomavirus-induced cervical carcinoma. | 1996 | 9373306 |
| assessment of cytotoxic t-lymphocyte phenotype using the specific markers granzyme b and tia-1 in cervical neoplastic lesions. | cervical carcinomas are closely associated with high-risk human papillomavirus (hpv) types and are preceded by cervical intraepithelial neoplasia (cin). most cin lesions regress spontaneously and will not evolve to invasive carcinoma. the cellular immune system mediated by cytotoxic t lymphocytes (ctls) and natural killer (nk) cells are thought to play an important role in the ultimate decline of cin lesions. although tia-1 is constitutively expressed in the majority of circulating t cells and d ... | 1997 | 9374383 |
| [recombinant therapeutic vaccines against cancer of the uterine cervix]. | several mechanism to evade the immune system are induced during cervical cancer development, including the decrease of expression of class i hla molecules and secretion of specific cytokines by tumoral cells. consequently, the stimulation of cytotoxic (ctl) and helper (th) t lymphocytes, as well as the natural killer (nk) cells and macrophages is very poor. the induction of immune response against tumors needs the stimulation of multiple components of the immune system: systemic stimulation of c ... | 1997 | 9381250 |
| epidemiology of human papillomavirus infections. | | 1997 | 9385310 |
| [human papillomavirus infection and esophageal squamous cell carcinoma]. | human papillomavirus (hpv) infection, especially high risk types hpv 16 and 18, have been studied widely in cervical cancer. however, hpv infection in esophageal cancer has not been well defined. in the present study, immunohistochemistry, pcr and southern blot hybridization methods were used to detect hpv infection in 127 cases of esophageal squamous cell carcinoma. immunohistochemistry results indicated that the virus was detected frequently in well differentiated carcinoma. the positive rates ... | 1996 | 9388862 |
| using polymerase chain reaction to human papillomavirus in oral and pharyngolaryngeal carcinomas. | increasingly, evidence has shown that human papillomavirus (hpv) plays a role in the induction of certain carcinomas. the presence of hpv sequences in 56 previously untreated oral and pharyngolaryngeal carcinomas was examined by the polymerase chain reaction (pcr). | 1997 | 9395012 |
| protective anti-tumor immunity induced by vaccination with recombinant adenoviruses encoding multiple tumor-associated cytotoxic t lymphocyte epitopes in a string-of-beads fashion. | vaccines harboring genes that encode functional oncoproteins are intrinsically hazardous, as their application may lead to introduction of these genes into normal cells and thereby to tumorigenesis. on the other hand, oncoproteins are especially attractive targets for immunotherapy of cancer, as their expression is generally required for tumor growth, making the arisal of tumor variants lacking these antigens unlikely. using murine tumor models, we investigated the efficacy of polyepitope recomb ... | 1997 | 9405669 |
| volume-activated taurine transport is differentially activated in human cervical cancer ht-3 cells but not in human papillomavirus-immortalized z183a and normal cervical epithelial cells. | 1. previous studies demonstrate that volume-sensitive chloride currents are distinctly activated in cervical cancer cells, but not in human papillomavirus (hpv)-immortalized and normal cervical cells. in the present study, the na(+)-independent volume-activated transport of taurine in three cervical cell types was investigated. 2. osmotic swelling of cervical cancer ht-3 cells suspended in na(+)-free hypotonic medium led to increased membrane uptake of taurine. this taurine uptake was effectivel ... | 1997 | 9406659 |
| seasonal fluctuations in the cervical smear detection rates for (pre)malignant changes and for infections. | the detection of diseases can exhibit seasonal fluctuations. this can be studied in cervical smears. over a 9-year observation span (january 1983-january 1992) a series of 504,093 cervical smears obtained from a routine cytology laboratory in the netherlands were examined for infections (monilia, trichomonas, actinomyces, human papilloma virus [hpv], chlamydia, and herpes) as well as for mild, moderate, and severe dysplasias, carcinoma in situ, and squamous carcinoma. statistical analysis (princ ... | 1997 | 9407207 |
| human papillomavirus detection in high-grade squamous intraepithelial lesions. comparison of hybrid capture assay with a polymerase chain reaction system. | the validity of human papillomavirus (hpv) detection using the hybrid capture assay (hca) was compared with the polymerase chain reaction (pcr) in 38 patients with high-grade squamous intraepithelial lesions (hsils). hca and pcr showed 84% agreement for hpv detection. hca missed a significant higher proportion of hsil compared with pcr (21% vs. 5%; p = .04). thus, the sensitivity of hca should be increased before this test can be recommended for hsil. | 1995 | 9407223 |
| the role of high risk hpv lymph node positivity in the surgical staging of cancer of the uterine cervix. | the presence of oncogenic human papillomavirus (hpv) dnas in cervical carcinomas and their normal and metastatic pelvic lymph nodes is studied worldwide. does the hpv positivity without histological metastasis in the lymph nodes mean an early prognostic indicator in the progress of the disease? the authors analyzed the surgical, histological and virological data of four cervical cancer patients, whose recurrence occurred very early and died soon. after performing radical hysterectomy with precis ... | 1997 | 9408386 |
| differential regulation of the pocket domains of the retinoblastoma family proteins by the hpv16 e7 oncoprotein. | the human papillomavirus e7 oncoprotein binds to the retinoblastoma (rb) tumor suppressor protein, and the binding to rb correlates with the oncogenic potential of e7. recent studies from several laboratories indicated that the half-life of the rb protein is reduced in cells that are stably transformed with e7, suggesting that e7 could induce the proteolytic degradation of rb. to investigate whether the rb degradation is a primary effect of e7 or a result of altered cell phenotype, we sought to ... | 1997 | 9419416 |
| human papillomavirus distribution in cervical tissues of different morphology as determined by hybrid capture assay and pcr. | distribution of various types of genital human papillomavirus (hpv) in smears from histologically classified cervical lesions was determined by hybrid capture assay (hca) and was compared with a polymerase chain reaction (pcr) system using general primers (gp) in first and type specific primers (ts) in a second step. the overall agreement of high-risk hpv by hca and the more sensitive gp/ts pcr was 80.6% (204 of 253, kappa value 0.6). human papillomavirus frequency by gp/ts pcr was 14-20% higher ... | 1997 | 9421083 |