| a selective and extremely potent antagonist of the neurokinin-1 receptor. | sendide [tyr6,d-phe7,d-his9]-substance p(6-11) has been examined by measurements of ligand binding to crude membrane fractions and by functional tests on the spinally mediated behavioral response. sendide potently displaced [3h]-labeled substance p (sp) binding to mouse spinal cord membranes in a competitive manner. in vivo, sendide, intrathecally co-injected with sp, competitively antagonized sp-induced scratching, biting and licking. the behaviors elicited by physalaemin, septide and [sar9, me ... | 1992 | 1280526 |
| effects of fr 113680 and fk 224, novel tachykinin receptor antagonists, on cigarette smoke-induced rat tracheal plasma extravasation. | we examined the effects of novel tachykinin antagonists, fr 113680 (n alpha-[n alpha-(n alpha-acetyl-l-threonyl)-n1-formyl-d-tryptophyl]-n- methyl-n-phenylmethyl-l-phenylalaninamide) and fk 224 (n-[n2-[n-[n-[n-[2,3-didehydro-n-methyl-n-[n[3-(2-phenthylpheny l) - propionyl]-l-threonyl]-tyrosyl]-l-leucynyl]-d-phenylalanyl]-l-allo - threonyl]-l-asparaginyl]-l-serine nu-lactone) on rat tracheal plasma extravasation induced by cigarette smoke. intravenous injection of fk 224 (0.032-3.2 mg kg-1) inhib ... | 1992 | 1280594 |
| neurogenic inflammation and the oral mucosa. | since the rôle of neurogenic inflammation in various pathological conditions is well-established, we presume that it also has great importance in the development of several inflammatory processes in the oral mucosa. in the course of experimentally-induced neurogenic inflammation of the oral mucosa in rats, vasodilation and plasma extravasation occur in the area supplied by unmyelinated capsaicin-sensitive fibres. upon activating these fibres in the oral mucosa, mediators such as histamine, subst ... | 1992 | 1280655 |
| effects of long-term laxative treatment on neuropeptides in rat mesenteric vessels and caecum. | in order to investigate the toxic effects of long-term treatment with anthraquinone laxatives, rats were fed either chocolate alone, or chocolate adulterated with senna or danthron (1,8-dihydroxyanthraquinone) for 5 months. mesenteric blood vessels and the outer muscle layers of the caecum, together with the myenteric plexus, were examined using ultrastructural, histochemical, immunohistochemical and immunoassay techniques. there was no ultrastructural evidence of degeneration in either the mese ... | 1992 | 1280681 |
| protective effect of cicletanine on hypertension-induced decreases in the renal kallikrein-kinin and prostaglandin systems in stroke-prone spontaneously hypertensive rats. | we investigated the effect of two oral (p.o.) doses of cicletanine (5 and 30 mg/kg/day) for 4 weeks on urinary excretion (uke), renal concentration (rkc) of kallikrein, and prostaglandin e2 (pge2) and 6-keto-pgf1 alpha urinary excretion of stroke-prone (sp) spontaneously hypertensive rats (shr) and wistar-kyoto rats (wky) rats submitted to a high sodium intake (1%). both doses of cicletanine induced a significant antihypertensive effect in treated shr as compared with hypertensive untreated cont ... | 1992 | 1280717 |
| [pro9]sp and [pglu6, pro9]sp(6-11) interact with two different receptors in the guinea-pig ileum as demonstrated with new sp antagonists. | structural considerations led us to postulate that the introduction of the dipeptides dpro9-pro10 and dpro9-meleu10 should lock the c-terminal tetrapeptide of sp in a type ii' beta-turn structure, a prerequisite for antagonist activity. indeed, as the gr 71251, [dpro9, pro10, trp11]sp was more potent in inhibiting the septide, (pa2 = 6.5), than the [pro9]sp, (pa2 < or = 5), spasmogenic activity in the guinea-pig ileum bioassay. this result confirms that septide, [pglu6, pro9]sp(6-11), a peptide ... | 1992 | 1280787 |
| central substance p increased blood pressure, heart rate and splanchnic nerve activity in anaesthetized rats without impairment of the baroreflex regulation. | in anaesthetized rats the baroreflex was checked before and 15 min after i.c.v. administration of 10 micrograms sp. the baroreflex was checked indirectly by relating both the reflex prolongation in heart period (inter-beat-interval: ibi) and the reflex inhibition of sna to a pharmacologically induced bp rise. after i.c.v. administration of sp (n = 10) the resting values of the bp increased significantly from 73 +/- 16 mm hg to 86 +/- 9 mm hg (diastolic pressure) and from 98 +/- 20 mm hg to 113 + ... | 1992 | 1280788 |
| an after-depolarization following action potentials and its modulation by substance p in rat sympathetic neurons. | sympathetic neurons in rat coeliac-superior mesenteric ganglia (c-smg) displayed an after-depolarization (adp) following action potentials and after-hyperpolarization. the adp had amplitudes of 3-10 mv and lasted for 2-6 s, during which membrane resistance and excitability of c-smg neurons increased. the reversal potential of adp was dependent on external k+ concentrations. the adp was suppressed in a solution containing cd2+ or zero ca2+. the adp thus appears to be produced by inhibition of cer ... | 1992 | 1280796 |
| structure determination of two new amino acid-containing derivatives of adenosine from trna of thermophilic bacteria and archaea. | two new nucleosides have been identified in unfractionated transfer rna of two thermophilic bacteria, thermodesulfobacterium commune, and thermotoga maritima, six hyperthermophilic archaea, including pyrobaculum islandicum, pyrococcus furiosus and thermococcus sp. and two mesophilic archaea, methanococcus vannielii and methanolobus tindarius. structures were determined primarily by mass spectrometry, as 3-hydroxy-n-[[(9-beta-d-ribofuranosyl-9h-purin-6- yl)amino]carbonyl]norvaline, (hn6a), struct ... | 1992 | 1280806 |
| distinct presynaptic control of dopamine release in striosomal- and matrix-enriched areas of the rat striatum by selective agonists of nk1, nk2, and nk3 tachykinin receptors. | using a sensitive in vitro microperfusion method, the effects of selective and potent agonists of nk1, nk2, and nk3 tachykinin receptors ([pro9]sp, ([lys5,meleu9,nle10]nka-(4-10), and [pro7]nkb, respectively) on the presynaptic control of dopamine release were investigated in striosomal-enriched (area rich in [3h]naloxone binding sites) and matrix-enriched areas of the rat striatum. marked differences could be demonstrated as follows: (i) when used at 0.1 microm, the nk1 agonist stimulated the r ... | 1992 | 1280822 |
| capsaicin releases calcitonin gene-related peptide from the human iris and ciliary body in vitro. | slices of human iris or ciliary body, obtained post-mortem (8-12 h after death, n = 5), were superfused in vitro with capsaicin (10 microm) and the immunoreactivity for substance p (sp-li) or calcitonin gene-related peptide (cgrp-li) was measured in the effluent. in the iris and in the ciliary body cgrp-li was 3.71 +/- 0.74 pmol/g and 3.01 +/- 0.55 pmol/g and sp-li was 6.68 +/- 0.75 pmol/g and 6.55 +/- 0.84 pmol/g, respectively. a first exposure to capsaicin increased the cgrp-li outflow from th ... | 1992 | 1280843 |
| influence of substance p on gastrointestinal myoelectrical activity in the conscious piglet. | in conscious piglets with electrodes implanted in the wall of the antrum pylori, the small intestine and the caecum, the influence of intravenous infusion of substance p (sp) (0.1 and 1 micrograms/kg/min for 2 hours) on gastrointestinal myoelectrical activity and arterial pressure was studied. sp was without significant influence on electrical activity of the antrum. however, intestinal myoelectrical activities were significantly stimulated. in the small intestine sp infusion resulted in an incr ... | 1992 | 1280892 |
| noradrenergic and peptidergic innervation of secondary lymphoid organs: role in experimental rheumatoid arthritis. | noradrenergic (na) and peptidergic nerve fibres are present in both primary and secondary lymphoid organs, distributing with the vasculature, trabecular and capsular smooth muscle, and within the parenchyma among cells of the immune system. na nerve terminals directly abut lymphocytes and macrophages in spleen and lymph nodes. in these organs, norepinephrine has fulfilled the basic criteria for neurotransmission with cells of the immune system as targets. in vitro and in vivo studies have demons ... | 1992 | 1281104 |
| increased expression of preprotachykinin, calcitonin gene-related peptide, but not vasoactive intestinal peptide messenger rna in dorsal root ganglia during the development of adjuvant monoarthritis in the rat. | neuropeptides in dorsal root ganglia (drg) have been implicated in the pathogenesis of pain and neurogenic inflammation in experimental and clinical arthritis. recently we demonstrated increased levels of substance p (sp) and calcitonin gene-related peptide (cgrp) confined to innervating drg in adjuvant-mediated monoarthritis. we have now investigated whether changes in peptide content are reflected in altered neuropeptide gene expression and the time course involved. using in situ hybridization ... | 1992 | 1281253 |
| partial purification and characterization of an endo-alpha-n-acetylgalactosaminidase from the culture medium of streptomyces sp. oh-11242. | for the purification of a new type of endo-alpha-n-acetylgalactosaminidase from the culture medium of streptomyces sp. oh-11242 (endo-galnac-ase-s) [iwase, ishii, ishihara, tanaka, omura & hotta (1988) biochem. biophys. res. commun. 151, 422-428], a method for assaying enzyme activity was established. using purified pig gastric mucus glycoprotein (pgm) as the substrate, oligosaccharides liberated from pgm were pyridylaminated, and the reducing terminal sugars of oligosaccharides larger than gal ... | 1992 | 1281406 |
| immunohistochemical studies on the intrinsic pancreatic nerves in the chicken. | a peroxidase anti-peroxidase method or an avidin-biotinylated complex method was used to visualize neural elements immunostained for several neuropeptides in the chicken pancreas. pancreatic ganglion cells were only immunoreactive with vasoactive intestinal polypeptide (vip), galanin and substance p (sp) antisera. vip-immunoreactive (ir) ganglion cells were the most numerous, and most of them also showed the distinct immunoreaction with galanin. vip- and galanin-ir nerve fibers were observed in ... | 1992 | 1281486 |
| amino terminus of substance p potentiates kainic acid-induced activity in the mouse spinal cord. | sensitization to the behavioral effects produced by repeated injections of kainic acid (ka) into the mouse spinal cord area has been previously shown to be abolished by pretreatment with capsaicin, a neurotoxin of substance p (sp)-containing primary afferent c-fibers. while sp has a variety of well characterized biological actions that are mediated by interactions of its cooh terminus with neurokinin receptors, more recently we have characterized an amino-terminally directed sp binding site. the ... | 1992 | 1281498 |
| [neurobiology of physical environmental stress]. | although effects of physical environmental stress, including noise and whole-body vibration, on human psychological activities and emotion are not negligible for environmental and occupational hygiene, attempts to elucidate their physiological and biomedical mechanisms have been not made until recently. neurobiological researches on the effects of the physical environment, e.g., noise and whole-body vibration on organisms were reviewed. it has been well accepted that such effects can be classifi ... | 1992 | 1281511 |
| enhanced responsiveness of ovalbumin-sensitized guinea-pig alveolar macrophages to tachykinins. | 1. we have evaluated the ability of substance p (sp), neurokinin a (nka) and the selective nk2 receptor agonist [beta-ala8]-nka(4-10) to induce superoxide anion (o2-) production and prostanoid (prostaglandin e2, thromboxane b2) release from alveolar macrophages (ams) isolated from control or actively sensitized guinea-pigs. 2. the dose-response curves for nka and sp were shifted to the left (three orders and one order of magnitude, respectively) in ams isolated from sensitized animals, with no v ... | 1992 | 1281723 |
| characterization of nk-1 receptors in guinea pig and rat brain membranes with nk-1 peptides and a non-peptide antagonist. | the major finding of the present investigation is the demonstration of different nk-1 receptors in rat and guinea pig brain membranes with cp 96345 (non-peptide nk-1 antagonist) and r-544 (nk-1 peptide antagonist). we used [3h][sar9,met(o2)11]sp, the highly selective ligand for nk-1 receptor to compare nk-1 binding sites in rat and guinea pig brain membranes. scatchard analysis revealed the existence of a single population of [3h][sar9,met(o2)11]sp binding sites in both preparations. the affinit ... | 1992 | 1281744 |
| electroconvulsive stimuli and brain peptides: effect of modification of seizure duration on neuropeptide y, neurokinin a, substance p and neurotensin. | we studied the effects of modification of duration of seizures induced by electroconvulsive stimuli (ecs) on the changes in concentration of neuropeptide y (npy), neurokinin a (nka), substance p (sp) and neurotensin (nt)-like immunoreactivity (-li) in specific rat brain regions. rats were divided into groups pretreated with saline, indomethacin, flurbiprofen or diazepam prior to either six sham ecss or six ecss. after sacrifice by focused microwave irradiation, brains were dissected into frontal ... | 1992 | 1281745 |
| autoradiographic analysis of 125i-substance p binding in rat spinal cord following chronic constriction injury of the sciatic nerve. | using receptor binding and autoradiographic techniques, changes in bolton-hunter labeled 125i-substance p (125i-bh-sp) binding were determined in laminae i/ii, v and x of rat lumbar spinal cord after chronic constriction injury (cci) of the sciatic nerve. when compared to the sham-operated side of the control group, sp binding significantly increased ipsilateral to the cci in laminae i/ii at 5, 10 and 20 days after injury and in lamina v at 5 days after injury. scatchard analysis was performed o ... | 1992 | 1281746 |
| functional and immunological responses of jurkat lymphocytes transfected with the substance p receptor. | 1. we have transfected the rat substance p receptor (spr) cdna into the leukemic t-lymphocyte cell line jurkat (j-wt) in order to study the effects of substance p (sp) on lymphocyte signaling mechanisms and the resultant neuropeptide-induced immunological changes. 2. the spr cdna was transfected into j-wt by the method of electroporation. clones expressing sprs were selected using a functional assay that measured sp-induced mobilization of intracellular ca2+ ([ca2+]i) in a fluorescence activated ... | 1992 | 1281754 |
| measles virus-substance p receptor interaction: jurkat lymphocytes transfected with substance p receptor cdna enhance measles virus fusion and replication. | 1. we have demonstrated previously (harrowe et al., 1990), using a lymphoblastoid cell line that constitutively expresses the substance p receptor (spr) (payan et al., 1984, 1986), that this receptor may facilitate measles virus (mv) fusion with these cells. in order to test this hypothesis further, a stable cell line transfected with spr cdna has been established, and various stages of mv infection in spr positive and negative cells compared. 2. jurkat cells, a human t-lymphoblastoid cell line, ... | 1992 | 1281755 |
| local secretion of corticotropin-releasing hormone in the joints of lewis rats with inflammatory arthritis. | corticotropin-releasing hormone (crh), the principal regulator of the hypothalamic-pituitary-adrenal axis, is also secreted in peripheral inflammatory sites, where it acts as a local proinflammatory agent. arthritis-susceptible lew/n rats have profoundly deficient hypothalamic crh responses to inflammatory stimuli and other stressors. arthritis-resistant f344/n rats, on the other hand, have a robust increase in hypothalamic crh in response to the same stimuli. contrasting with these hypothalamic ... | 1992 | 1281840 |
| expression of growth-associated protein 43 and nerve growth factor receptor in human skin: a comparative immunohistochemical investigation. | the growth-associated protein 43 (gap43) is a neuronal membrane protein involved in axonal growth and regeneration as well as in the modulation of synaptic plasticity. it is present in sensory and sympathetic neurons, where it is consistently associated with the expression of nerve growth factor receptor (ngfr). we investigated, by means of immunohistochemistry, the presence and distribution of the gap43-immunoreactivity (ir) and of the ngfr-ir in the adult normal human skin from various body re ... | 1992 | 1281863 |
| the capsaicin test in mice for evaluating tachykinin antagonists in the spinal cord. | a capsaicin test involving peripheral nociception, which produces behaviour similar to that elicited by formalin, is described in mice. capsaicin was injected subcutaneously (s.c.) into the dorsal surface of a hindpaw and the time the animals spent licking the paw was recorded. doses of capsaicin of 6.25-1600 ng induced nociception, during a period of 5 min, starting immediately after injection and disappearing completely at 10 min. intrathecally (i.t.) administered [d-arg1,d-trp7,9,leu11]substa ... | 1992 | 1281912 |
| characterization of monoclonal antibodies against eosinophil chemotactic factors from young adult worms of angiostrongylus cantonensis. | two kinds of igg1 monoclonal antibodies against eosinophil chemotactic factors (ecfs) derived from angiostrongylus cantonensis young adult worms (ya) were established. western blot analyses demonstrated that one monoclonal antibody recognized a 16.1 kd component of ya-whole worm extract, and the other an 85 kd component. these antibodies did not show any cross reactions against several helminth antigens, as assessed by elisa. the chemotactic activity of ya-whole worm extract for guinea-pig-eosin ... | 1992 | 1281918 |
| chitinous components of the cell wall of fusarium oxysporum. | the cell wall of fusarium oxysporum f. sp. lycopersici was digested with chitinase to analyze the structure of its chitinous components. in spite of a similar acetylation degree of the cell wall components to that of 25-35% acetylated chitosan, only n-acetylglucosamine disaccharide [(glcnac)2] was obtained from chitinase hydrolyzate of the fungal cell wall by cm-sephadex c-25 column chromatography, while (glcnac)2 and several types of deacetylated chitooligosaccharides were separated from that o ... | 1992 | 1282049 |
| pharmacological profile of a high affinity dipeptide nk1 receptor antagonist, fk888. | 1. in our search for compounds that inhibit the binding of [3h]-substance p (sp) to guinea-pig lung membranes, the dipeptide sp antagonist, fk888, was developed by chemical modification of the parent compound, (d-pro4, d-trp7,9,10, phe11)sp4-11. 2. in a [3h]-sp binding assay using guinea-pig lung membranes and rat brain cortical synaptic membranes, fk888 displaced [3h]-sp binding with a ki value of 0.69 +/- 0.13 nm and 0.45 +/- 0.17 microm, respectively, in a competitive manner. 3. fk888 inhibit ... | 1992 | 1282073 |
| effect of substance p on circadian rhythms of firing activity and the 2-deoxyglucose uptake in the rat suprachiasmatic nucleus in vitro. | the suprachiasmatic nuclei (scn) have been identified as a pacemaker for many circadian rhythms in mammals. although substance p (sp) fibers from retina are found to terminate the scn, the physiological role of this peptide is uncertain. the 2-deoxyglucose (2-dg) uptake and firing activity in the scn show a robust circadian change. sp causes an increase in 2-dg uptake by scn during the subjective night but not during subjective day. sp-induced increase in 2-dg uptake is blocked by co-treatment w ... | 1992 | 1282077 |
| delta-opioid-receptor activation by [d-pen2,d-pen5]enkephalin and morphine inhibits substance p release from trigeminal nucleus slices. | the release of substance p (sp) from spinal dorsal horn slices is partially inhibited by micromolar concentrations of selective delta-opioid receptor agonists. in the present study, we have examined the effect of nanomolar concentrations of [d-pen2,d-pen5]enkephalin (dpdpe, delta-opioid receptor agonist) and low micromolar of concentrations morphine on k(+)-evoked sp release from rat trigeminal nucleus caudalis (tnc) slices. dpdpe and morphine inhibited sp release with an apparent maximal effect ... | 1992 | 1282103 |
| effect of the nep inhibitor sch32615 on airway responses to intravenous substance p in guinea pigs. | we examined the effects of the selective neutral endopeptidase (nep) inhibitor sch32615 on airway responses to rapid intravenous infusions of substance p (sp) and neurokinin a (nka) and on recovery of administered tachykinins from arterial blood in anesthetized mechanically ventilated guinea pigs. sch32615, in doses that cause a marked increase in the magnitude of bronchoconstriction induced by infused nka, had little effect on the changes in pulmonary conductance (gl) or dynamic compliance indu ... | 1992 | 1282124 |
| comparison in different tissue preparations of the in vitro pharmacological profile of rp 67580, a new non-peptide substance p antagonist. | we describe the effects of rp 67580, a new non-peptide substance p (sp) antagonist, on tachykinin-induced contractions of guinea-pig ileum, trachea and urinary bladder, rabbit pulmonary artery and rat portal vein. all nk1 agonists tested (sp, septide, spome and [pro9]sp) contracted guinea-pig ileum, trachea and urinary bladder (pd2 = 7.5 to 9.1), but they had no effect on rabbit pulmonary artery or rat portal vein (pd2 < 6). rp 67580 inhibited these effects: guinea-pig ileum, pa2 = 7.1 to 7.6; g ... | 1992 | 1282222 |
| transmitter substances contained in the petrosal ganglion cells determined by a double-labeling method in the rat. | the presence of glutamate (glu), aspartate (asp) and substance p (sp) in the petrosal ganglion of rats anesthetized with pentobarbital sodium was studied using retrograde labeling of the carotid sinus nerve (csn) with horseradish peroxidase (hrp) in combination with immunohistochemistry. (i) the incidence of hrp/glu-labeled cells was the highest (32%, n = 3), followed in order by hrp/asp-labeled cells (23%, n = 3) and hrp/sp-labeled cells (6%, n = 3). (ii) no significant difference was observed ... | 1992 | 1282228 |
| analysis of the gene encoding the rna subunit of ribonuclease p from cyanobacteria. | the genes encoding the rna subunit of ribonuclease p from the unicellular cyanobacterium synechocystis sp. pcc 6803, and from the heterocyst-forming strains anabaena sp. pcc 7120 and calothrix sp. pcc 7601 were cloned using the homologous gene from anacystis nidulans (synechococcus sp. pcc 6301) as a probe. the genes and the flanking regions were sequenced. the genes from anabaena and calothrix are flanked at their 3'-ends by short tandemly repeated repetitive (strr) sequences. in addition, two ... | 1992 | 1282240 |
| blockade of electrical field stimulation-induced nonadrenergic noncholinergic (nanc) excitatory response of the guinea pig ileum. | determination of guinea pig ileal response to electrical field stimulation (efs) before and after complete adrenergic and cholinergic blockade showed that the nonadrenergic noncholinergic excitatory (nanc-e) response contributed 40% to the total contractile response. nanc-e responses were reproducible to consecutive identical efs. tetrodotoxin significantly blocked the nanc-e response. the nanc-e responses obtained before and after treatment of the tissues with receptor antagonists of histamine ... | 1992 | 1282271 |
| the relationship of calbindin-containing neurons with substance p, leu-enkephalin and cholecystokinin fibres: an immunohistochemical study in the rat thalamus. | in the rat thalamus, immunoreactivity for the calcium binding protein calbindin (cb) is mostly confined to neuronal cell bodies, sometimes revealing proximal dendrites, of the midline, intralaminar and posterior regions. substance p (sp)-, cholecystokinin (cck)- and leu-enkephalin (l-enk)-immunoreactive (ir) elements in the thalamus are fibre-like structures, intermingled with punctate elements probably representing axonal arborizations and their synaptic boutons. these peptidergic fibres are un ... | 1992 | 1282325 |
| postnatal development of sympathetic and sensory innervation of the rhesus monkey ovary. | we have used immunofluorescence to study the postnatal development of the sympathetic and sensory innervation to the rhesus monkey (macaca mulatta) ovary. sympathetic nerves were identified as adrenergic by their content of tyrosine hydroxylase (th)-like immunoreactivity and as peptidergic by the presence of neuropeptide y (npy). fibers containing substance p (sp) or calcitonin gene-related peptide (cgrp)-like immunoreactivity were considered as sensory, whereas vasoactive intestinal peptide (vi ... | 1992 | 1282372 |
| purification and characterization of a novel thermostable lipase from pseudomonas cepacia. | a thermostable lipase from pseudomonas cepacia has been purified to homogeneity as judged by sds-page and isoelectric focusing. the purification included treatment of the culture supernatant with acrinol, hydrophobic interaction chromatography, and gel filtration. the enzyme was a monomeric protein with m(r) of 36,500 and pi of 5.1. the optimal ph at 50 degrees c and optimal temperature at ph 6.5 were 5.5-6.5 and 55-60 degrees c, respectively, when olive oil was used as the substrate. simple tri ... | 1992 | 1282513 |
| smooth muscle dilatation in the human uterine artery induced by substance p, vasoactive intestinal polypeptide, calcitonin gene-related peptide and atrial natriuretic peptide: relation to endothelium-derived relaxing substances. | the relaxatory influences of substance p (sp), vasoactive intestinal polypeptide (vip), calcitonin gene-related peptide (cgrp) and atrial natriuretic peptide (anp) were investigated in human uterine arteries precontracted by noradrenaline in vitro. sp, vip, cgrp and anp all relaxed isolated uterine arteries with intact endothelium. when tested on vessels devoid of their endothelium vip and sp had no effect on smooth muscular tone, while anp and cgrp still induced unchanged vasodilatation. these ... | 1992 | 1282518 |
| influence of spinal cord hemisection on the configurational changes in motor and primary afferent neurons and the chemical messenger alterations in the rat lumbar segments. | cholera toxin b subunit (ctb), a retrograde transport marker, was injected into the rat gastrocnemius muscles, and changes in ctb-labeling pattern of motoneurons and primary afferent neurons at the level l4 and l5 after spinal cord hemisection of the l1 level were observed in conjunction with the alterations of chemical messengers such as serotonin (5-ht), calcitonin gene-related peptide (cgrp), substance p (sp), galanin (gal), met-enkephalin (enk), and neuropeptide y (npy). motoneurons at the l ... | 1992 | 1282529 |
| effects of lordosis-relevant neuropeptides on midbrain periaqueductal gray neuronal activity in vitro. | certain neuropeptides can facilitate lordosis by acting on midbrain periaqueductal gray (pag) in estrogen-primed female rats. here, we investigated responses of individual pag neurons in vitro, to five neuropeptides: substance p (sp), luteinizing hormone-releasing hormone (lhrh), prolactin (prl), oxytocin (ot), and thyrotropin-releasing hormone (trh). substance p, ot, and trh excited spontaneous activity of pag neurons through neurotransmitter-like actions in a dose-dependent manner, whereas lhr ... | 1992 | 1282709 |
| glycerol injection to the rat trigeminal nerve: histological and immunohistochemical studies. | the effect of topical glycerol injection into the rat trigeminal nerves was investigated histologically and immunohistochemically. anhydrous glycerol was injected into the preganglionic portion of the trigeminal nerves via a ventral approach. extensive myelin swelling and axonolysis were observed in the rats killed 1 and 2 weeks after glycerol injection. numerous inflammatory cells were seen especially in the animals sacrificed 1 week after surgery. myelin disintegration continued up to 4 weeks ... | 1992 | 1282768 |
| cerebrovascular effects of substance p after experimental subarachnoid haemorrhage. | the vasoactive effects of substance p (sp), as well as the content of cyclic guanine monophosphate (cgmp), were determined in the rabbit basilar artery after subarachnoid haemorrhage (sah). out of 47 rabbits, 24 were subjected to a sah, induced by injecting 5 ml of autologous arterial blood into the cisterna magna; 23 were used as controls. in 20 animals (10 sah and 10 controls), isometric tension recording of isolated rings of the basilar artery--dissected 2 days after sah--was employed to asse ... | 1992 | 1282769 |
| prostatic specific antigen and immunoglobulin binding factor in human seminal plasma and prostate. | antibodies raised against prostatic specific antigen (psa) and immunoglobulin binding factor (igbf) of human seminal plasma (sp) were used to localize the antigens in various tissues by western blot. both antigens were found only in the prostate, including benign prostatic hypertrophy and prostatic adenocarcinoma. the polyclonal anti-psa antibodies stained five prostatic protein bands with estimated m(r) values of 10, 14, 22, 25, and 33 kd, whereas anti-igbf antibodies stained a single 16-kd pro ... | 1992 | 1282794 |
| excitation of cutaneous afferent nerve endings in vitro by a combination of inflammatory mediators and conditioning effect of substance p. | a broad mixture of inflammatory mediators ("inflammatory soup") was used to investigate the responsiveness of primary afferents from rat hairy skin in an in vitro skin-saphenous nerve preparation. in addition, a conditioning effect of the tachykinin substance p on chemosensitivity of nociceptors was examined. inflammatory soup (is) was made up in synthetic interstitial fluid from bradykinin, serotonin, histamin and prostaglandin e2 (all 10(-5) m). in addition, the potassium and the hydrogen ion ... | 1992 | 1282891 |
| n omega-nitro-l-arginine blocks the second phase but not the first phase of the endothelium-dependent relaxations induced by substance p in isolated rings of pig carotid artery. | endothelium-dependent relaxations can be evoked by a variety of stimuli, among them substance p (sp), which is found in sensory nerve fibers supplying the adventitia-media junction of most muscular arteries. this study determined the role of endothelium-derived nitric oxide as a mediator of endothelium-dependent relaxations to sp in isolated rings of the pig carotid artery suspended in organ chambers for isometric tension recording. sp (10(-12)-10(-7) m) caused concentration-dependent relaxation ... | 1992 | 1282940 |
| biphasic response to substance p in canine basilar arteries. | the responses to intraluminally applied substance p (sp) were examined in isolated and perfused canine basilar arteries using the stainless-steel cannula inserting method. in control vessels with intact endothelium, this peptide induced a monophasic dilation at lower doses, and a biphasic response, i.e., an initial dilation followed by a secondary constriction at higher doses. after extraluminal treatment with oxyhemoglobin, the dilation was attenuated and the constriction was augmented. after e ... | 1992 | 1282941 |
| distribution and origin of extrinsic nerve fibers containing calcitonin gene-related peptide, substance p and galanin in the rat upper rectum. | the distributions of nerve fibers containing calcitonin gene-related peptide (cgrp), substance p (sp) and galanin (gal) were examined in the rat rectum of mutants rats, aganglionic rats (agrs), which completely lack the intramural nerve cells in the large intestine, and of their normal littermates. the origin of extrinsic peptide-containing nerve fibers was examined using retrograde tracing combined with immunohistochemistry in normal rats. in the rectum of normal rats, cgrp-, sp- and gal-immuno ... | 1992 | 1283008 |
| regulation of human t lymphoblast growth by sensory neuropeptides: augmentation of cholecystokinin-induced inhibition of molt-4 proliferation by somatostatin and vasoactive intestinal peptide in vitro. | the effects on proliferation of molt-4 lymphoblasts of cholecystokinin (cck-8), somatostatin-14 (ss), vasoactive intestinal peptide (vip) and substance p (sp) were investigated using different combinations of the peptides, peptide analogs and their antagonists. in vitro proliferation of the cells was measured by a colorimetric assay for cell growth and survival. results indicate that sp and sp (3-11) stimulated, whereas cck-8, vip and ss inhibited, proliferation in a dose-dependent manner (p < 0 ... | 1992 | 1283156 |
| substance p and neurokinin a immunoreactive nerve fibres in the developing salivary glands of the rat. | the time of appearance and distribution of substance p (sp) and neurokinin a (nka) immunoreactive nerve fibres in developing salivary glands of the rat were studied by the use of indirect immunohistochemical methods. the glands were examined at daily intervals from the 15th day in utero (i.u.) until birth, and subsequently on the 2nd, 5th, 7th, 12th, 16th and 30th postnatal day. the findings were compared to samples from adult. the first sp- and nka-immunoreactive (ir) nerve fibres appeared on t ... | 1992 | 1283162 |
| calcitonin gene-related peptide, substance p and gap-43/b-50 immunoreactivity in the normal and arthrotic knee joint of the mouse. | the aim of this study was to describe the normal distribution of calcitonin gene-related peptide (cgrp) and substance p (sp) containing fibres in the knee joint of the mouse and to obtain insight into the changes in innervation associated with degenerative processes in the joint. arthrosis was induced by a single subpatellar intra-articular injection of bacterial collagenase. after decalcification in edta solutions, the cgrp and sp fibres were visualized by peroxidase-antiperoxidase pre-embeddin ... | 1992 | 1283163 |
| neurite regeneration in the cat recurrent laryngeal nerve: an immunohistochemical study. | the recurrent laryngeal nerve (rln) consists of various motor, sensory and autonomic nerve fibers, although it has not been established whether different neuronal types exhibit a similar ability to regenerate. to address this question, freezing was used to injure the cat rln fibers and the presence or absence of immunoreactivity for neuropeptides or transmitter-synthesizing enzymes was then examined as a marker to classify the fibers. in the control rln, calcitonin gene-related peptide-immunorea ... | 1992 | 1283303 |
| antibodies to the rat substance p receptor: production and characterization. | 1. a protein a-rat substance p receptor (spr) fusion protein was genetically engineered and used as an immunogen to raise a polyclonal antiserum to the spr. the fusion protein was expressed in escherichia coli driven by the heat-inducible lambda promoter (lambda pr). 2. the fusion protein was purified using an igg-sepharose column, which specifically binds proteins containing the protein a moiety. the igg fraction obtained after the immunization was cleaved to produce fab fragments, which were s ... | 1992 | 1283362 |
| endothelium-derived relaxing factor inhibits platelet adhesion to cultured porcine endocardial endothelium. | by using a simple platelet binding assay, we investigated whether endothelium-derived relaxing factor (edrf) released from endocardial endothelium influences the adhesion of unstimulated platelets to these cells. under basal conditions 8.0 +/- 0.32% of total platelets added adhered. the nitric oxide (no) synthase inhibitor, i.e. ng-nitro l-arginine methyl ester (l-name), and the edrf inhibitor haemoglobin (hb) increased this adhesion, but another no synthase inhibitor, ng-monomethyl l-arginine ( ... | 1992 | 1283374 |
| polyclonal antibodies against the rat nk1 receptor: characterization and localization in the spinal cord. | we have developed antibodies against the nk1 receptor and have investigated its cellular distribution. rabbit polyclonal antibodies were generated against peptide (19-32) of the rat brain nk1 receptor. they were very specific to the nk1 site as shown by elisa against various epitopes of nk1, nk2 and nk3 receptors and by immunoblotting of proteins from bacteria transfected with rat brain nk1 receptor cdna and from rat cortex. determining how immunostained nk1 receptors are distributed in the rat ... | 1992 | 1283531 |
| modulatory role of substance p on gonadotropin and prolactin secretion in the rabbit. | substance p (sp) is present in large quantities in the brainstem and hypophysiotropic areas of the brain, but its roles in gonadotropin and prolactin secretion are controversial. the aim of this study was to measure luteinizing hormone (lh), follicle-stimulating hormone (fsh), and prolactin (prl) release from the pituitary after either intracerebroventricular (icv) injection or infusion of sp or its c- and n-terminal fragments in intact (int) and ovariectomized (ovx) conscious rabbits. a single ... | 1992 | 1283536 |
| substance p stimulates il-1 production by astrocytes via intracellular calcium. | there is increasing evidence that local substance p (sp) exacerbates peripheral inflammations, partly by stimulating production of inflammatory cytokines such as interleukin-1 (il-1) and tumor necrosis factor alpha (tnf alpha). sp may play similar roles in certain central nervous system inflammations. multiple sclerosis plaques, for example, form around veins which are innervated by unmyelinated sp-containing fibers, and astrocytes in multiple sclerosis plaques stain for sp. we tested whether sp ... | 1992 | 1283556 |
| numerous sp-positive pyramidal neurons in cat neocortex are glutamate-positive. | an immunocytochemical technique that allows visualization of two antigens in the same neuron was used to verify the possibility that some neocortical pyramidal neurons contain both glutamate (glu) and substance p (sp) immunoreactivity. the results show that a large fraction of sp-positive pyramidal neurons are also glu-positive, and indicate that in a small population of cortical neurons a fast excitatory synaptic transmitter and a slow peptidic modulator coexist. | 1992 | 1283558 |
| intrathecal oxymetazoline does not produce neurotoxicity in the spinal cord of the rat. | to determine if intrathecal (i.t.) oxymetazoline (oxy) induces histological evidence of spinal neurotoxicity, male, sprague-dawley rats (300-450 g; implanted with an i.t. catheter) were treated with i.t. saline or 100 nmol oxy twice daily for 3 days, or 200 or 300 nmol oxy once daily for 3 days. spantide (d-arg1, d-try7,9, leu11-substance p; 0.067 nmol = 0.1 microgram, 0.167 nmol = 0.25 microgram or 0.334 nmol = 0.5 microgram) or capsaicin (0.164 mumol = 50 micrograms), given as a single i.t. in ... | 1992 | 1283563 |
| agonist-antagonist interactions in the skin: comparison of effects of loratadine and cetirizine on skin vascular responses to prick tests with histamine and substance p. | the skin vascular responses (weal, flare, blood flow measurements) elicited by intradermal administration by pricking of histamine (hs) and substance p (sp) were evaluated 6 h after a single intake of anti-h1 agents displaying different activity profile on skin tests at currently recommended dosages (loratadine 10 mg, cetirizine 10 mg) as compared to placebo (p). the weal and flare response and the increases of blood flow occurring in the usual flare area after hs and sp were almost completely a ... | 1992 | 1283698 |
| heterogenous distribution of peptide-containing nerve fibres within the circular muscle layer of the human pylorus. | the distribution of nerve fibres immunoreactive for vasoactive intestinal polypeptide (vip), substance p (sp), methionine-enkephalin (enk), calcitonin gene-related peptide (cgrp) and neuropeptide y (npy) within the circular muscle layer was examined histochemically in the human pylorus, adjacent antrum and duodenum. longitudinal cryostat sections of the pyloric and surrounding regions were stained by an indirect immunofluorescence method, and the total length of each type of peptide-containing f ... | 1992 | 1283961 |
| effect of substance p on respiratory rhythm and pre-inspiratory neurons in the ventrolateral structure of rostral medulla oblongata: an in vitro study. | the pre-inspiratory (pre-i) neurons which fire in the pre- and usually also during the post-inspiratory phase are located in the ventrolateral structures of the rostral medulla. they are suggested as primary rhythm generating neurons for respiration. these have been studied in isolated brainstem-spinal cord preparations from newborn 0-5-day-old rats. we have found that application of substance p (sp) enhanced the respiratory rhythm as measured by c4 ventral root and pre-i neuronal activities. fu ... | 1992 | 1283971 |
| immunocytochemical localisation of substance p in vagal ganglion cells and pericellular arborisations in the monkey. | the quantitative cell count showed the presence of about 20,000 ganglion cells with associated satellite elements in the nodose ganglion in the monkey. among these closely packed cells, at least one-third were substance p (sp) immunoreactive, ranging from faint or moderate to intense staining. substance p immunoreactivity (sp-ir) was localised in the cell bodies and their long extending neurites. ultrastructural study showed that sp-ir was not associated with any particular organelles or inclusi ... | 1992 | 1284128 |
| effect of nitro-l-arginine on endothelium-dependent hyperpolarizations and relaxations of pig coronary arteries. | 1. endothelium-dependent relaxation is caused by an endothelium-derived relaxing factor (edrf) identified as nitric oxide (no). our objective was to test whether one or several distinct endothelium-dependent relaxing factors exist. 2. in pig coronary arteries, a hyperpolarization accompanied by the relaxation caused by high concentrations of substance p (sp) and bradykinin (bk). 3. to examine the role played by nitric oxide and prostacyclin in the endothelium-dependent relaxations and hyperpolar ... | 1992 | 1284311 |
| [development of a system for cloning a dna fragment, containing the determinant of resistance to actinomycin for streptomyces chrysomallus no.2--a producer of actinomycin c]. | to study the modes of actinomycin biosynthesis and the mechanism responsible for resistance to the antibiotic producing s. chrysomallus no. 2, the authors undertook an examination and studies into the cloning system for gene(s) of resistance to actinomycin from a s. chrysomallus no. 2 actinomycin c producer and the cloning of a s. chrysomallus no. dna fragment to the actinomycin-sensitive streptomyces sp. 26-115 h-i on the vector plasmid pij702. the cloning gave rise to actinomycin-resistant str ... | 1992 | 1284351 |
| [changed levels of substance p and somatostatin in hiv-positive children]. | substance p (sp) and somatostatin (som), so as other neuropeptides can modulate neurologic and immunologic functions. sp has been described to enhance both in vitro and in vivo immunoglobulin synthesis. on the contrary, som has an inhibitory effect on the same activity. the modulating effect is more evident on iga isotype. hypergammaglobulinemia and in particular high levels of iga is a common finding in pediatric aids and an imbalance among regulatory effects of neuropeptides might be suggested ... | 1992 | 1284355 |
| 5838-dni, a deoxyribonuclease inhibitor produced by streptomyces sp. strain no. a-5838. | 5838-dni, an inhibitor of deoxyribonuclease (dnase) ii from porcine spleen was produced by streptomyces sp. strain no. a-5838. the structure of 5838-dni was shown to be 1,4,4a,5,12,12a-hexahydro-4,4a,11,12a-tetrahydroxy-3,8-dimethoxy-9- methoxycarbonyl-10-methyl-1,5,12-trioxo naphthacene. although similar in structure to tetracenomycin c, which is an antibiotic against gram-positive bacteria, 5838-dni has different antibacterial activity. 5838-dni was distinguished from 5923-dni, a previously re ... | 1992 | 1284432 |
| met-enkephalin-arg6-gly7-leu8- and substance p-containing projections from the nucleus preopticus medianus to the paraventricular hypothalamic nucleus. | the nucleus preopticus medianus (pome) is known to be important for the regulation of fluid balance and cardiovascular control. direct projections from the pome to the paraventricular hypothalamic nucleus (pvn), where vasopressin-containing neurons exist, were examined in the rat using immunohistochemistry combined with a retrograde tract tracing method. after injection of wga-hrp-colloidal gold into the pvn, many neurons were retrogradely labeled in the pome; some of them were immunoreactive to ... | 1992 | 1284443 |
| [pain and stress: biological protection]. | the biological methods of animal organism protection, the role of stress and the nociceptive signals, the hierarchy of defence mechanism--from the autonomic reflexes, neuroendocrine regulations, emotional reactions and expressions up to peculiar to the human being intellectual safeguard have been considered. the latest data on the role of tachykinins in transmission have been examined. the discovery of mediator function of "substance p" (sp), other related peptides, which coexists and realizes f ... | 1992 | 1284617 |
| human cystic fibrosis transmembrane conductance regulator directed to respiratory epithelial cells of transgenic mice. | human cystic fibrosis transmembrane conductance regulator (cftr) was expressed in transgenic mice under the control of transcriptional elements derived from the human surfactant protein c (sp-c) gene. the hcftr mrna was expressed in lungs and testes: in the lung, we found hcftr mrna in bronchiolar and alveolar epithelial cells, and cftr protein in respiratory epithelial cells. while the level of expression of hcftr mrna varied, hcftr mrna and protein were detected in pulmonary epithelial cells o ... | 1992 | 1284640 |
| [the role of substance p in the modulation of myotension in paralyzed limbs after spinal transsection on rats]. | it is a common clinical observation that stretch reflex excitability increases progressively after spinal transsection in man, and causes many severe complications. the purpose of the present study was to investigate the role and mechanism of substance p (sp) on the spinal reflex following spinal cord transsection. we first observed the changes of spontaneous electromyography (emg) recorded in the paralyzed limbs of spinally transsected rats, and found that the emg amplitude increased progressiv ... | 1992 | 1284659 |
| effects of trigeminal neurotransmitters on piglet pial arterioles. | we investigated effects of calcitonin gene-related peptide (cgrp), substance p (sp), and neurokinin a (nka) on pial arterioles in newborn pigs. pial arteriolar diameter was determined using a closed cranial window and intravital microscopy. initial diameters were approximately 100 microns. calcitonin-gene related peptide dilated pial arterioles by 22 +/- 8% at 10(-9)m and by 34 +/- 6% at 10(-8)m (n = 8), and this response was not significantly altered by prior administration of indomethacin (5mg ... | 1992 | 1284673 |
| enhanced learning produced by injection of neurokinin substance p into the region of the nucleus basalis magnocellularis: mediation by the n-terminal sequence. | the effect of unilateral injection of the neurokinin substance p (sp) and of certain n- or c-terminal sp-fragments into the region of the nucleus basalis magnocellularis (nbm) on inhibitory avoidance learning was investigated. rats with chronically implanted cannulae were tested on a one-trial uphill avoidance task. immediately after the training trial, rats were injected with 0.74 pmol sp or equimolar dosed sp(1-7), dime-c7, or sp(7-11). control groups included vehicle-injected rats and a group ... | 1992 | 1284861 |
| susceptibility of simulium (chirostilbia) pertinax kollar, 1832 (diptera, simuliidae) to bacillus thuringiensis var. israelensis in an atypical breeding habitat. | | 1992 | 1284991 |
| the motor actions of natural neurokinins and their specific agonists on the gastrointestinal tract of the rat. | neurokinins (nk) are a group of peptides that share a common c-terminal and play an important role in control of the motor functions of the mammalian gut. neurokinin receptors such as nk1, nk2 are certainly present in any segment of gut, whereas the nk3 receptors are probably present in the ileum of the guinea pig and duodenum of the rat. we measured gastrointestinal responses with natural nk and their very specific agonists to determine the probable receptors in the stomach and small intestine ... | 1992 | 1285005 |
| [influence of 5.7-dihydroxytryptamine on electro-acupuncture analgesia and substance p level in central nervous system of the arthralgic rats]. | the relation between electroacupuncture (ea) analgesia (a) and substance p (sp) level in the brain stem (bs) and lumbar spinal cord (lsc) of arthralgic rats was investigated. the rats were divided into three groups: 1)5.7-dihydroxytryptamine (5.7-dht) + ea, 2) vehicle (v) + ea, and 3)5.7-dht. all the animals were induced arthralgesia by injecting freund's adjuvant 7 days after cisterna injection of 5.7-dht or vehicle. the sp level in the bs and lsc was determined by ria. the results indicated th ... | 1992 | 1285044 |
| [ultrastructure identification of raphe-spinal serotonergic pain modulating system in rats]. | 5,6-dihydroxytryptamine was microinjected into the nucleus raphe magus (nrm) of rats. the sections of upper cervical segments were processed for immunoreactive substance p (sp). the degenerated axon terminals and immunoreactive fibers were identified by electron microscopy. the results show that the degenerated axon terminals and immunoreactive positive dendrites and axons were found in the laminae i and ii of the dorsal horn. the electron-dense axon terminals were in contact with unlabelled den ... | 1992 | 1285045 |
| antiinflammatory effect of a protein kinase c inhibitor (k-252a) on the development of the dextran-induced paw edema in the rat (preliminary results). | the effect of a metabolite of nocardiopsis sp. as a protein kinase c inhibitor from microbial origin was investigated on the onset and development of dextran-induced paw edema in the rat. it was published that this compound (k-252a) interferes with histamine release from mast cells, while dextran-induced paw and nose edema are induced by vasoactive agents, like histamine etc., released from the disrupted mast cells. the antiinflammatory effect of the k-252a is effectuated by the inhibition of pr ... | 1992 | 1285369 |
| [a new tachykinin receptor revealed by substance p analogues in the guinea pig ileum]. | [pro9]sp and septide have been described as selective agonists for the sp receptor (nk-1 type). these two peptides contract with a great efficacy the guinea-pig ileum, but unexpectedly septide was practically devoid of affinity for the nk-1 site labelled by 3h-[pro9]sp. like septide, sp analogues like sp-o-ch3, [apa9-10]sp and [pro9,10]sp share the same peculiar properties. in addition, the contracting activity of these peptides is not explained by an interaction with nk-2 or nk-3 sites. gr 71,2 ... | 1992 | 1285414 |
| myxozyma neotropica sp. nov. (candidaceae)--a new yeast species from costa rica. | two strains of an undescribed, soil-borne species of the genus myxozyma were recovered. they differ from other accepted species of this genus in their carbon assimilation pattern, mol% g+c and low extent of dna complementary. a description of the new species, myxozyma neotropica, and a key to the species accepted in the genus are given. | 1992 | 1285642 |
| clavispora opuntiae and other yeasts associated with the moth sigelgaita sp. in the cactus pilosocereus arrabidae of rio de janeiro, brazil. | clavispora opuntiae was the prevalent yeast associated with the feeding sites of sigelgaita sp. larvae in the cactus pilosocereus arrabidae. also associated with this habitat were candida sonorensis, pichia cactophila, pichia barkeri, candida sp. a, geotrichum sp., geotrichum sericeum and the yeast like organisms prototheca zopfii and acremonium sp. atypical yeast biotypes that may represent new species of pichia, sporopachydermia and candida were isolated. mating types of clavispora opuntiae we ... | 1992 | 1285643 |
| long-time survival and morphological stability of preserved sporothrix schenckii strains. | the survival and stability of morphological traits of 33 sporothrix schenckii strains were evaluated. strain subcultures were maintained under mineral oil for different periods of time lasting as long as 41 years, or in sterile distilled water for as long as 23 years. of the 33 strains preserved under mineral oil, 28 (85%) maintained viability and unchanged macro- and microscopic characteristics. all of the 4 strains maintained in distilled water also remained viable, with no morphological chang ... | 1992 | 1287484 |
| purification of the neurite outgrowth promoting fragment of mouse laminin. | a method for isolation of the neurite outgrowth promoting fragment of mouse laminin (fragment 8) is described in this paper. besides producing excellent yields, this method was shown to be fast and practical, since it is based on a single step which consists in an ion exchange chromatography of elastase digested laminin. | 1992 | 1287649 |
| expression of the phosphorylase kinase gamma subunit catalytic domain in escherichia coli. | the catalytic subunit of phosphorylase b kinase (gamma) and an engineered truncated form (gamma-trc, residues 1-297) have been expressed in escherichia coli. the truncated protein included the entire catalytic domain as defined by sequence alignment with other protein kinases but lacked the putative calmodulin binding domain. full-length protein was produced in insoluble aggregates. some activity was regenerated by solubilization in urea and dilution into renaturating buffer but the activity was ... | 1992 | 1287663 |
| fungal gut colonization with candida or pityrosporum sp. and serum candida antigen in preterm neonates with very low birth weights. | to evaluate the diagnostic value of gut colonization by yeasts and of candida antigen in serum for predicting fungal infection in very premature neonates, faecal and serum samples were obtained biweekly from 27 newborn babies treated at our neonatal intensive care unit. altogether 82 sets of serum and faecal samples were obtained. 17 babies were followed for > or = 4 weeks. blood cultures, both by routine and lysis centrifugation techniques, were performed for bacteria and fungi if infection was ... | 1992 | 1287812 |
| [brief psychotherapy in clinical medicine patients]. | the criteria that "illness is biographical crisis od the individual" and that the only medicine is "personal medicine" is stressed. clinical medicine, which covers medicine in its entirety, demands conceptual and doctrinal reaffirmations so that gradually the patient can come to be dealt with as a human being fron a holistic point of view, which commences with his complaint and consultation, continues with the interview and semiology, to finish with the diagnosis and therapy which, although in s ... | 1992 | 1288068 |
| a biosensor for l-proline determination by use of immobilized microbial cells. | a biosensor to quantify l-proline within 10(-5)-10(-3) mole/l concentration is described. immobilized pseudomonas sp. cells grown in a medium containing l-proline as the only source of carbon and nitrogen were used to create the biosensor. the cells oxidized l-proline specifically consuming o2 and did not react with other amino acids and sugars. the change in oxygen concentration was detected with a clark oxygen membrane electrode. the cells were immobilized by entrapment in polyvinyl alcohol (p ... | 1992 | 1288409 |
| similarity of the constituents between glomerular arteriolar and mesangial lesions in experimental diabetes. | in our previous studies in experimental diabetic rats, we have observed close similarities of ultrastructure and accumulation of igg and igm between the mesangial expansion and arteriolar hyalinosis of the glomerulus, and have presumed that both diabetic lesions are essentially of similar nature. in the present study, we carried out a further study on the constituents of both these lesions, using the pa-tch-sp-pd technique for neutral carbohydrates, sialic acid and glycoproteins and the igg-gold ... | 1992 | 1289017 |
| cutaneous involvement of systemic fungal infections in neutropenic patients. | skin nodules can be the first evidence of a disseminated fungal infection in febrile neutropenic patients. we present our experience in the diagnosis of this clinical problem in five patients treated for serious hematologic conditions in our service. there were two cases of candida sp., two of fusarium sp., and one of trichosporon sp.. the immediate assessment of any suspicious lesion, including a biopsy of the lesion for microbiological and histopathologic examinations, will usually lead to the ... | 1992 | 1289191 |
| interaction of pulmonary surfactant-associated protein (sp-a) with surfactant lipids. | a pulmonary surfactant-associated protein complex with components of 36, 32 and 28 kda was isolated from human lung homogenates and reassembled with surfactant lipids prepared as small unilamellar liposomes. the role of divalent cations in the assembly of this recombinant lipoprotein complex was studied by monitoring the changes in turbidity, intrinsic tryptophanyl fluorescence and surface activity. the protein-facilitated lipid aggregation was promoted on addition of 5 to 20 mm ca2+. intrinsic ... | 1992 | 1289235 |
| the trigemino-pupillary reflex: a model of sensory-vegetative integration. | trigeminal stimulation can induce pupillary changes. in vivo and in vitro studies have demonstrated that electrical impulses applied at the trigeminal level can provoke a miotic response, whose nature has been ascribed to the anti-dromic release of neuropeptides (substance p in particular). in order to better define the pupil response to trigeminal stimulation, we investigated the human pupil response to quantified (painless and painful) corneal stimuli by means of a combined (neurophysiological ... | 1992 | 1289382 |
| sensitivity status of plasmodium falciparum to chloroquine, amodiaquine, quinine, mefloquine and sulfadoxine/pyrimethamine in a tribal population of district sundargarh, orissa. | in a malaria-endemic area of orissa, wherein chloroquine has been in use for over thirty years, 58.3% (14/24) p. falciparum cases did not respond to single dose chloroquine (10 mg base/kg) in in-vivo test. with standard dose (25 mg base/kg) 31.2% cases (10/32) showed resistance, i.e. at ri (15.6%), rii (9.4%) and riii (6.2%) levels. standard dose was superior in response to the single dose therapy [p < 0.05; chi 2 (df 1) = 4.11]. out of eight isolates tested in vitro, two showed resistance to ch ... | 1992 | 1291342 |
| an experimental transmission of porcine strains of blastocystis sp. in the laboratory mice and gerbils. | outbred laboratory mice were successfully infected with porcine strains of blastocystis sp. using faecal stages as well as stages from fresh or passaged cultures. in contrast, inbred balb/c mice and gerbils were only rarely infected and the intensity of their infection was mostly negligible. blastocystis sp. was never observed inside the host cells. these results indicate a low host specificity of blastocystis sp. as well as different sensitivity of investigated hosts to blastocystis sp. infecti ... | 1992 | 1291454 |
| gyrodactylus bohemicus sp. n. (monogenea: gyrodactylidae) from oncorhynchus mykiss (walbaum) and salvelinus fontinalis (mitchill) (clupeiformes: salmonidae) in czechoslovakia. | gyrodactylus bohemicus sp. n (gyrodactylidae: monogenea) is described from the fins, skin and gill filaments of oncorhynchus mykiss (walbaum) and salvelinus fontinalis (mitchill) (clupeiformes: salmonidae) from a trout farm in czechoslovakia. g. bohemicus sp. n. is most closely related to g. thymalli zitnan, 1960 and g. magnus konovalov, 1967 in the shape of the anchors, the ventral and the dorsal bar, but can be distinguished from both these species by the shape of the marginal hook proper. | 1992 | 1291456 |
| vaginal yeast flora of pregnant women in the cusco region of peru. | a study of the vaginal yeast flora in pregnant women living in cusco and in its region (peru), located approximately 3000 m above sea level, is reported. we observed 300 pregnant, healthy and non-diabetic women who attended a gynaecological clinic in the lorena, regional or ipss (instituto peruano de seguridad social) hospitals in cusco. a comprehensive clinical history was obtained from each patient. it included age, work, parity, time of pregnancy, use of contraceptives or antibiotics, type of ... | 1992 | 1291874 |
| vaccines for control of fertility. | major developments in birth control vaccines are on the horizon. the human chorionic gonadotropin (hcg) vaccine has entered phase ii clinical trials after successful completion of phase i studies at 5 centers in india and 4 centers abroad. it is the most advanced vaccine of its type in the world. the trials are being conducted on women of proven fertility who are sexually active. the available results indicate the efficiency of the vaccine to prevent pregnancy in women at or above titres of 50 n ... | 1992 | 1293038 |
| flavobacterium indologenes infection in leopard frogs. | an investigation of an epidemic of infectious disease in a frog (rana pipiens) colony was conducted. six of 40 frogs in a continuous (once through) water flow housing system had weight loss, swollen abdomen, corneal edema, uveitis, subcutaneous edema, petechial hemorrhage, incoordination, and respiratory distress. the frogs had lesions consistent with bacterial septicemia. a gram-negative, nonfermenting bacillus, flavobacterium indologenes (flavobacterium sp biovar iib), was isolated in pure cul ... | 1992 | 1293124 |
| [antimalarial effect of n-hentriacontanol isolated from cuatresia sp (solanaceae)]. | the antimalarial activity of the fatty alcohol, n-hentriacontanol, isolated from the bolivian solanaceae, cuatresia sp, is investigated in vivo through a classical four-day suppressive test against plasmodium berghei and p. vinckei in mice. this product markedly reduced the virulence of experimentally induced p. vinckei infection. n-hentriacontanol belongs to a new class of antimalarial natural compounds to be exploited for therapeutic purposes. | 1992 | 1294019 |