Publications

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nonfermenting gram-negative bacilli infections in a tertiary care hospital in kolar, karnataka.aim: nonfermenting gram-negative bacilli (nfgnb), which are saprophytic in nature, have emerged as important healthcare-associated pathogens. they exhibit resistance not only to beta lactam and the other groups of antibiotics, but also to carbapenems. this study was undertaken to identify the nonfermenters isolated from various clinical samples, to assess their clinical significance, to know the type of healthcare-associated infections they caused, and to know their anti-microbial sensitivity pa ...200921938252
Contemporary unconventional clinical use of co-trimoxazole.Clin Microbiol Infect 2012; 18: 8-17 ABSTRACT: In the late 1960s, the combination of trimethoprim and sulphamethoxazole (co-trimoxazole) was introduced into clinical practice and used to treat many infectious diseases, such as urinary tract infections, respiratory infections, sexually transmitted diseases, Gram-negative sepsis, enteric infections and typhoid fever. Subsequently, co-trimoxazole was reported to be effective against numerous bacterial, fungal and protozoal pathogens, including Noca ...201221851485
contribution of resistance-nodulation-division efflux pump operon smeu1-v-w-u2-x to multidrug resistance of stenotrophomonas maltophilia.kj09c, a multidrug-resistant mutant of stenotrophomonas maltophilia kj, was generated by in vitro selection with chloramphenicol. the multidrug-resistant phenotype of kj09c was attributed to overexpression of a resistance nodulation division (rnd)-type efflux system encoded by an operon consisting of five genes: smeu1, smev, smew, smeu2, and smex. proteins encoded by smev, smew, and smex were similar to the membrane fusion protein, rnd transporter, and outer membrane protein, respectively, of kn ...201121930878
multidrug-resistant gram-negative bacterial infections after liver transplantation - spectrum and risk factors.objectives: gram-negative bacilli infections, especially multidrug-resistant gram-negative bacilli infections, are the leading cause of high mortality after liver transplantation. this study sought to investigate the type of infection, infection rate, pathogenic spectrum, antibiotic-resistance profile, risk factors, and epidemiology of multidrug-resistant gram-negative bacterial infection. methods: a retrospective cohort study was conducted and data of 217 liver transplant patients receiving cad ...201122198738
Characteristics of microbial aerosols released from chicken and swine feces.Bioaerosols generated during livestock and poultry production are significant occupational hazards. This study investigates the characteristics of bioaerosols released from animal feces. Fresh feces from pigs and chickens were obtained and tested in a controlled-environment facility. Airborne viable (culturable) bacteria and fungi were sampled hourly for 48 hr. The predominant species were identified via polymerase chain reaction analysis. The number of bacterial colonies released from chicken f ...201121874960
Tsunami lung.We encountered three cases of lung disorders caused by drowning in the recent large tsunami that struck following the Great East Japan Earthquake. All three were females, and two of them were old elderly. All segments of both lungs were involved in all the three patients, necessitating ICU admission and endotracheal intubation and mechanical ventilation. All three died within 3 weeks. In at least two cases, misswallowing of oil was suspected from the features noted at the time of the detection. ...201122057370
Genotyping of environmental and clinical Stenotrophomonas maltophilia isolates and their pathogenic potential.Stenotrophomonas maltophilia is a highly versatile species with useful biotechnological potential but also with pathogenic properties. In light of possible differences in virulence characteristics, knowledge about genomic subgroups is therefore desirable. Two different genotyping methods, rep-PCR fingerprinting and partial gyrB gene sequencing were used to elucidate S. maltophilia intraspecies diversity. Rep-PCR fingerprinting revealed the presence of 12 large subgroups, while gyrB gene sequenci ...201122110692
Minimal Inhibitory Concentration of Ceftazidime and Co-trimoxazole for Stenotrophomonas Maltophilia using E-test.Stenotrophomonas maltophilia, previously named as Pseudomonas or Xanthomonas maltophilia, is an important nosocomial pathogen201121887057
[the first experience of application of pcr techniques in real-time mode to diagnose bacteriemia during postoperational period in cardiosurgery patients].the results comparison is made between pcr polymerase chain reaction techniques in real-time mode and cultural techniques of analysis of blood samples in patients with suspicion of bacteriemia during early postoperational period. from september to december 2009 43 blood samples from 37 adult patients aged 59+9.2 years were analyzed on suspicion of bacteriemia. the blood samples for pcr analysis (septifast technique roche diagnostics, switzerland) were taken parallel to the inoculation. among 43 ...201122164420
The Ax21 protein is a cell-cell signal that regulates virulence in the nosocomial pathogen Stenotrophomonas maltophilia.Stenotrophomonas maltophilia encodes proteins related to the Rax proteins of Xanthomonas oryzae, which are required for the synthesis and secretion of the Ax21 protein. Here we show that Ax21 acts as a cell-cell signal to regulate a diverse range of functions, including virulence, in this nosocomial pathogen.201121908663
Reducing Sugar-Producing Bacteria from Guts of Tenebrio Molitor Linnaeus (Yellow mealworm) for Lignocellulosic Waste Minimization.The guts of Tenebrio Molitor Linnaeus (yellow mealworm) were used as inocula to isolate reducing sugar-producing bacteria during bioconversion of lignocellulose to reducing sugars in this study. Three carbon sources, i.e., carboxymethyl cellulose (CMC), filter paper (FP), and lignocellulosic waste (LIG), were specifically selected; and two types of culturing media (M1 and M2) were used. After 6 months of sequential cultivation, lignocellulose (i.e., polysaccharides) degradation of enrichments M1 ...201121869568
[Community acquired Stenotrophomonas maltophilia soft-tissue infection in a previously healthy patient.] 201121944651
characterization of copper-resistant rhizosphere bacteria from avena sativa and plantago lanceolata for copper bioreduction and biosorption.copper is a toxic heavy metal widely used to microbial control especially in agriculture. consequently, high concentrations of copper residues remain in soils selecting copper-resistant organisms. in vineyards, copper is routinely used for fungi control. this work was undertaken to study copper resistance by rhizosphere microorganisms from two plants (avena sativa l. and plantago lanceolata l.) common in vineyard soils. eleven rhizosphere microorganisms were isolated, and four displayed high res ...201122002857
exploring the in situ accessibility of small subunit ribosomal rna of members of the domains bacteria and eukarya to oligonucleotide probes.the principle that the small subunit ribosomal rna (ssu rrna) is generally accessible to oligonucleotide probes designed to have high thermodynamic affinity was tested with stenotrophomonas maltophilia, rhodobacter sphaeroides, bacillus subtilis, and saccharomyces cerevisiae. fluorescein-labeled probes, designed to have δg(overall)°=-14±1 and to avoid the potential of nucleobase-specific quenching, were used to target 20 randomly selected sites in each organism. a site was considered accessible ...201222264799
gut-associated bacteria throughout the life cycle of the bark beetle dendroctonus rhizophagus thomas and bright (curculionidae: scolytinae) and their cellulolytic activities.dendroctonus rhizophagus thomas and bright (curculionidae: scolytinae) is an endemic economically important insect of the sierra madre occidental in mexico. this bark beetle has an atypical behavior within the genus because just one beetle couple colonizes and kills seedlings and young trees of 11 pine species. in this work, the bacteria associated with the dendroctonus rhizophagus gut were analyzed by culture-dependent and culture-independent methods. analysis of 16s rrna sequences amplified di ...201222234511
nagz-dependent and nagz-independent mechanisms for the β-lactamases expression in stenotrophomonas maltophilia.the β-n-acetylglucosaminidase (nagz), encoded by nagz gene, is a critical enzyme for the basal-level ampc derepression (ampc expression in absence of β-lactam challenge) in ampd and dacb mutants of pseudomonas aeruginosa. three mutants with a phenotype of basal-level l1 and l2 β-lactamase derepression in stenotrophomonas maltophilia have been reported, including kjδdi (ampd(i) mutant), kjδmrca (mrca mutant), and kjδdiδmrca (ampd(i) and mrca double mutant). in this study, nagz of s. maltophilia w ...201222252801
multilocus sequence typing for clonality analysis of antimicrobial-resistant stenotrophomonas maltophilia strains. 201222259772
the role of nutrients in the biodegradation of 2,4,6-trinitrotoluene in liquid and soil.the widely used explosive 2,4,6-trinitrotoluene (tnt) has residues that are potentially explosive, toxic, and mutagenic. tnt and other explosives can be degraded by microorganisms; however, biostimulation is needed for process efficiency. to investigate the effectiveness of using biostimulation to degrade tnt, we added varying concentrations of a nutrient amendment consisting of inorganic salts, plant extracts, and molasses to soil and liquid media. for the inoculum we used a consortium of bacte ...201222245864
stenotrophomonas maltophilia: an emerging global opportunistic pathogen.summary: stenotrophomonas maltophilia is an emerging multidrug-resistant global opportunistic pathogen. the increasing incidence of nosocomial and community-acquired s. maltophilia infections is of particular concern for immunocompromised individuals, as this bacterial pathogen is associated with a significant fatality/case ratio. s. maltophilia is an environmental bacterium found in aqueous habitats, including plant rhizospheres, animals, foods, and water sources. infections of s. maltophilia c ...201222232370
isolation and characterization of lipase-producing bacteria in the intestine of the silkworm, bombyx mori, reared on different forage.abstract the silkworm, bombyx mori l. (lepidoptera: bombycidae), an oligophagous insect that mainly feeds on mulberry leaves, is susceptible to entomopathogen infection when reared with tricuspid cudrania leaves. a total of 56 dominant bacterial strains, classified into 12 phylotypes based on bacteriological properties and analysis of 16s rrna genes, were isolated from the intestine of the fourth and fifth instar silkworm larvae. ten and seven phylotypes exist in the intestine of the silkworm ...201122243438
endowashers: an overlooked risk for possible post-endoscopic infections.background: prevention of post-endoscopy infections is an important objective to assure patient safety. endowashers, or high throughput irrigation water pumps, are a frequently used device on endoscopes. recommendations published by professional bodies and regulatory health agencies cover not only adequate reprocessing of fiber-endoscopes but also state accepted methods of regular microbial sampling. although major instruments like endoscopes are covered by these recommendations, other devices u ...201122242094
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