Publications

TitleAbstractYear
Filter
PMID
Filter
molecular survey of haemoplasmas in shelter dogs and associations with rhipicephalus sanguineus sensu lato.this study aimed to assess the occurrence of canine haemoplasma infection in domestic dogs and its possible trans-stadial transmission by rhipicephalus sanguineus sensu lato (ixodida: ixodidae) in shelter dogs in diyarbakır province in southeast turkey. blood samples (n = 282) collected from domestic dogs were analysed by polymerase chain reaction (pcr) for the presence of canine haemoplasma. fully engorged nymphs (n = 204) were removed from dogs that were positive for canine haemoplasma by pcr ...201728685834
a retrospective study of the characterization of rickettsia species in ticks collected from humans.rickettsiae (family rickettsiaceae, order rickettsiales) are obligate intracellular bacteria transmitted by arthropod vectors. several rickettsia species causing vector-borne rickettsioses belong to the spotted fever group (sfg). traditionally, rickettsia conorii has been considered as the main etiologic agent of mediterranean spotted fever. however, the molecular characterization of rickettsiae allowed identifying other species involved in spotted fever in the mediterranean region. in this stud ...201728457821
israeli spotted fever in sicily. description of two cases and minireview.mediterranean spotted fever (msf) is endemic in italy, where rickettsia conorii subsp. conorii was thought to be the only pathogenic rickettsia and rhipicephalus sanguineus the vector and main reservoir. r. conorii subsp. israelensis, which belongs to the r. conorii complex, is the agent of israeli spotted fever (isf); apart from israel, it has also been found in italy (sicily and sardinia) and in different regions of portugal. we describe here two severe cases of isf which occurred in otherwise ...201728408252
spotted fever: epidemiology and vector-rickettsia-host relationship in rio de janeiro state.the eco-epidemiological scenario of spotted fever (sf), a tick-borne disease that affects humans and other animals in several countries around the world, was analyzed in rio de janeiro (rj) state, brazil. during the last 34 years, 990 sf cases were reported in rj (the brazilian state with the highest population density), including 116 cases confirmed by serology (rifi) or pcr, among 42.39% of the municipalities with reported cases of sf. the epidemiologic dynamics of sf in rj state are very hete ...201728424664
rickettsia rickettsii infecting rhipicephalus sanguineus sensu lato (latreille 1806), in high altitude atlantic forest fragments, ceara state, brazil.in brazil, spotted fever (sf) is caused by rickettsia rickettsii and rickettsia parkeri strain atlantic forest. in recent years, several human cases of a milder sf have been reported from the maciço de baturité region of ceará state. previous studies in this region found r. parkeri strain atlantic forest to be present in rhipicephalus sanguineus sensu lato and amblyomma ovale ticks. the present study isolated and identified the rickettsia spp. present in this new endemic area in brazil. in march ...201728535905
vector competence of amblyomma americanum (acari: ixodidae) for rickettsia rickettsii.rickettsia rickettsii - the etiologic agent of rocky mountain spotted fever (rmsf) - is widely spread across the americas. in the us, dermacentor spp. ticks are identified as primary vectors of r. rickettsii and rhipicephalus sanguineus s.l. has been implicated in transmission of this pathogen in several locations in the southwest. conversely, ticks of the genus amblyomma are recognized vectors of rmsf in central and south america, but not in the us. a. americanum is one of the most aggressive h ...201728433728
direct evidence of rickettsia typhi infection in rhipicephalus sanguineus ticks and their canine hosts.murine typhus is a rickettsiosis caused by rickettsia typhi, whose transmission is carried out by rat fleas in urban settlements as classically known, but it also has been related to cat fleas in a sub-urban alternative cycle that has been suggested by recent reports. these studies remarks that in addition to rats, other animals like cats, opossums and dogs could be implied in the transmission of rickettsia typhi as infected fleas obtained from serologically positive animals have been detected i ...201728652984
in vitro repellency of deet and β-citronellol against the ticks rhipicephalus sanguineus sensu lato and amblyomma sculptum.rhipicephalus sanguineus sensu lato and amblyomma sculptum can parasite humans and domestic animals and are vectors of pathogens, including zoonoses. repellents are an important tool of tick control. this study aimed to compare the efficacy of n,n-diethyl- 3-methylbenzamide (deet), a standard repellent, versus β-citronellol in a petri dish bioassay. a semicircle of filter paper (31.8cm(2)) was treated with 87μl of one of four concentrations (0.200, 0.100, 0.050 and 0.025mg/cm(2)) of β-citronello ...028495195
efficacy of tagetes minuta (asteraceae) essential oil against rhipicephalus sanguineus (acari: ixodidae) on infested dogs and in vitro.ticks from rhipicephalus sanguineus complex are widely distributed in the world and one species from this complex is the most common tick on dogs in brazil, notably in urban areas. this tick is a vector of several diseases. among others it transmits the agent of canine ehrlichiosis, a major dog infectious disease and the agent of rocky mountain spotted fever. this tick can spread rapidly and develop intolerable infestations within no time. currently tick control is done with acaricides and deman ...027815656
on-host control of the brown dog tick rhipicephalus sanguineus latreille (acari: ixodidae) by metarhizium brunneum (hypocreales: clavicipitaceae).ticks are obligatory blood-sucking arthropods. their life cycle includes a relatively short period of feeding on a vertebrate host and a long off-host period spent in the upper layer of the soil. entomopathogenic fungi are known to be highly effective tick pathogens and the on-host application of these fungi may be a promising economic approach for tick control. in this study, we evaluated the tick control provided by spraying metarhizium brunneum onto the tick's vertebrate host, specifically ge ...201323267821
molecular detection of anaplasma platys, anaplasma phagocytophilum and wolbachia sp. but not ehrlichia canis in croatian dogs.the bacteria anaplasma platys, anaplasma phagocytophilum and ehrlichia canis are tick-borne agents that cause canine vector-borne disease. the prevalence of these pathogens in south eastern europe is unknown with the exception of an isolated case of a. platys detected in a dog imported into germany from croatia. to gain a better insight into their presence and prevalence, pcr-based screening for these bacterial pathogens was performed on domesticated dogs from different regions of croatia. blood ...201728905230
co-circulation of crimean-congo hemorrhagic fever virus strains asia 1 and 2 between the border of iran and pakistan.crimean-congo hemorrhagic fever (cchf) is a tick-borne viral disease that is transmitted by numerous species of ticks, which serve both as a reservoir and vector of cchf virus (cchfv). molecular and serological tests were undertaken on hard ticks (ixodidae spp.) and samples from livestock were collected in 2015 from chabahar county in southeast iran. using rt-pcr, the ticks were tested for the presence of cchfv. in addition, seven livestock were serologically tested for the presence of igg antib ...201729159319
molecular detection of crimean-congo hemorrhagic fever virus in ticks, greece, 2012-2014.crimean-congo hemorrhagic fever virus (cchfv) is transmitted to humans mainly through the bite of infected ticks. in greece, only one clinical case has been observed, in 2008, but the seroprevalence in humans is relatively high (4.2%). to have a first insight into the circulation of cchfv in greece, 2000 ticks collected from livestock during 2012-2014 were tested. cchfv was detected in 36 of the 1290 (2.8%) tick pools (1-5 ticks per pool). two genetic lineages were identified: europe 1 and europ ...201728920177
rickettsia spp. among wild mammals and their respective ectoparasites in pantanal wetland, brazil.the genus rickettsia comprises obligatory intracellular bacteria, well known to cause zoonotic diseases around the world. the present work aimed to investigate the occurrence of rickettsia spp. in wild animals, domestic dogs and their respective ectoparasites in southern pantanal region, central-western brazil, by molecular and serological techniques. between august 2013 and march 2015, serum, whole blood and/or spleen samples were collected from 31 coatis, 78 crab-eating foxes, seven ocelots, 4 ...201829111373
molecular detection and identification of rickettsia species in ticks (acari: ixodidae) collected from belize, central america.little is known about tick-borne rickettsial pathogens in belize, central america. we tested ixodid ticks for the presence of rickettsia species in three of the six northern and western belizean districts. ticks were collected from domestic animals and tick drags over vegetation in 23 different villages in november 2014, february 2015, and may 2015. a total of 2,506 collected ticks were identified to the following species: dermacentor nitens neumann (46.69%), rhipicephalus sanguineus (latreille) ...201728981693
rhipicephalus sanguineus sensu lato (ixodidae) in synantropic rodents in rio grande do sul, brazil.rhipicephalus sanguineus, the brown dog tick, is responsible for maintaining and transmitting various pathogens, both in animals and human beings, and it is of great sanitary importance. this communication reports the first occurrence of rhipicephalus sanguineus sensu lato parasitizing rattus norvegicus in the state of rio grande do sul, brazil, and it is also the first record of this tick species parasitizing rattus rattus in brazil. the rodents were captured from the port area, located in the ...201425054514
laboratory evaluations of the immediate and sustained efficacy of lotilaner (credelio™) against four common species of ticks affecting dogs in north america.effective control of tick infestations on dogs is important to reduce the risk of transmission of bacterial, viral, and protozoal pathogens. laboratory studies were initiated to determine the efficacy of lotilaner against common ticks infesting dogs in the united states.201729089057
ixodidae fauna of domestic dogs in parana, southern brazil.the present study aimed to contribute towards identification and registration of tick species that parasitize dogs in rural and urban areas of three mesoregions of paraná, southern brazil, and to estimate the rate of occurrence of each species. fifty-six dogs with ticks living in three mesoregions: metropolitana de curitiba (mc), centro oriental (cop) and centro sul paranaense (csp), were used in the study. from these 56 dogs, 253 ticks were collected and were identified and morphologically char ...201728678895
contributions to the knowledge of hard ticks (acari: ixodidae) in colombia.the known tick fauna of colombia includes 58 species (15 argasidae and 43 ixodidae). to add to the knowledge of the biology of ticks in colombia, hard ticks (ixodidae) were collected from domestic animals or vegetation during 2014-2016 in 10 of colombia's departments. ticks were identified to species through morphological examinations. taxonomic identification was confirmed for some specimens by molecular methods, including phylogenetic analyses inferred from three tick genes (cytochrome c oxida ...201829055642
tick-borne infections in dogs and horses in the state of espírito santo, southeast brazil.this work aims to identify and quantify the percentage of rickettsia spp., ehrlichia spp., babesia spp. and hepatozoon spp. positive pet dogs, and to identify ticks collected on these animals in the state of espírito santo, in the southeast region of brazil. the study included 378 dogs, 226 females and 152 males, of various breeds and ages (mean age of 4.1 years). all animals were examined for ticks, and whole blood was collected and processed by conventional pcr protocols for babesia spp., anap ...201829279085
ticks and tick-borne pathogens of dogs along an elevational and land-use gradient in chiriquí province, panamá.systematic acarological surveys are useful tools in assessing risk to tick-borne infections, especially in areas where consistent clinical surveillance for tick-borne disease is lacking. our goal was to identify environmental predictors of tick burdens on dogs and tick-borne infectious agents in dog-derived ticks in the chiriquí province of western panama to draw inferences about spatio-temporal variation in human risk to tick-borne diseases. we used a model-selection approach to test the relati ...201728417248
a molecular survey of tick-borne pathogens from ticks collected in central queensland, australia.central queensland (cq) is a large and isolated, low population density, remote tropical region of australia with a varied environment. the region has a diverse fauna and several species of ticks that feed upon that fauna. this study examined 518 individual ticks: 177 rhipicephalus sanguineus (brown dog tick), 123 haemaphysalis bancrofti (wallaby tick), 102 rhipicephalus australis (australian cattle tick), 47 amblyomma triguttatum (ornate kangaroo tick), 57 ixodes holocyclus (paralysis tick), 9 ...201829336689
seasonal distribution and faunistic of ticks in the alashtar county (lorestan province), iran.ticks are non-permanent obligate parasites that have considerable medical-veterinary and zoonosis importance. in this regard a study designed to investigate the distribution and fauna of ticks in the alashtar county in iran from april and march 2014.201729187953
laboratory and field evaluation of brown dog tick behavioral responses to potential semiochemicals.the brown dog tick, rhipicephalus sanguineus sensu lato (parasitiformis: ixodidae), is an ectoparasite of dogs that can be found worldwide. this tick poses unique difficulties in management because it can complete its entire life cycle indoors and has demonstrated acaricide resistance. the ability to monitor for tick presence and abundance is necessary for developing effective control programs. as such, an evaluation of adult brown dog tick behavioral responses to 16 potential semiochemicals was ...201727914935
evaluation of four bed bug traps for surveillance of the brown dog tick (acari: ixodidae).the brown dog tick, rhipicephalus sanguineus (latrielle), can be a serious residential pest due to its unique ability, among ticks, to complete its lifecycle indoors. a single engorged and fertilized female tick can oviposit around 4,000 eggs, allowing indoor establishment to be rapid and easy to miss in early-stage infestations. acaricide treatment is currently the primary method of control, but can be costly and can lead to the development of acaricide resistance in tick populations. traps of ...201526336310
pathogens in ectoparasites from free-ranging animals: infection with rickettsia asembonensis in ticks, and a potentially new species of dipylidium in fleas and lice.vector-borne infections are persistent public health threats worldwide. in recent years, a number of mosquito-borne viruses have emerged or re-emerged to cause major disease outbreaks. other vector-borne pathogens, however, remain understudied and much neglected especially in the developing regions of the world including southeast asia. in this study, the brown dog tick rhipicephalus sanguineus sensu lato, cat louse felicola subrostratus, and cat fleas ctenocephalides felis and ctenocephalides o ...201728969827
the sustained speed of kill of ticks (rhipicephalus sanguineus) and fleas (ctenocephalides felis felis) on dogs by a spot-on combination of fipronil and permethrin (effitix®) compared with oral afoxolaner (nexgard®).the rapid speed of kill of a spot-on, combination of fipronil-permethrin (effitix®, virbac) was shown against infestations of rhipicephalus sanguineus and ctenocephalides felis on dogs. efficacy was determined against new infestations at weekly intervals for one month after treatment. dogs were allocated randomly to either an untreated control or to a single administration, given on day 0, of either topical fipronil-permethrin (6.7-13.4mg/kg and 60-120mg/kg, respectively) or oral afoxolaner (2.7 ...201728807310
comparative analysis of storage conditions and homogenization methods for tick and flea species for identification by maldi-tof ms.ticks and fleas are vectors for numerous human and animal pathogens. controlling them, which is important in combating such diseases, requires accurate identification, to distinguish between vector and non-vector species. recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof-ms) was applied to the rapid identification of arthropods. the growth of this promising tool, however, requires guidelines to be established. to this end, standardization protocols ...201728722283
diversity and distribution of ticks from domestic ruminants in lebanon.ticks (acari: ixodidae) are ectoparasites infesting livestock in every geographic area in the world and they are vectors of several viral, bacterial, and protozoan pathogens to animals and humans worldwide. a deep knowledge of the geographical distribution of these arthropods would have a key role in the control of tick-borne diseases. few data are available about tick presence in domestic ruminants in lebanon. the study aimed at providing an analysis of tick presence and distribution in lebanon ...201728675252
a randomized, controlled study to assess the efficacy and safety of lotilaner (credelio™) in controlling ticks in client-owned dogs in europe.oral administration of lotilaner flavoured chewable tablets (credelio™, elanco) to dogs has been shown to provide a rapid onset of killing activity of infesting ticks, with sustained efficacy for at least 35 days. a study was undertaken in europe to confirm lotilaner's safety and anti-tick efficacy in client-owned dogs.201729089058
laboratory evaluations of the immediate and sustained effectiveness of lotilaner (credelio™) against three common species of ticks affecting dogs in europe.there is a continuing need for novel approaches to tick control in dogs. one such approach lies in the ability of lotilaner (credelio™), an isoxazoline with a rapid onset of action, to provide sustained efficacy against ticks. two studies were undertaken to confirm lotilaner's efficacy, at the minimum dose rate of 20 mg/kg, against the three most common tick species in europe.201729089050
effect of recombinant glutathione s-transferase as vaccine antigen against rhipicephalus appendiculatus and rhipicephalus sanguineus infestation.the ticks rhipicephalus appendiculatus and rhipicephalus sanguineus are the main vectors of theileria parva and babesia spp. in cattle and dogs, respectively. due to their impact in veterinary care and industry, improved methods against r. appendiculatus and r. sanguineus parasitism are under development, including vaccines. we have previously demonstrated the induction of a cross-protective humoral response against rhipicephalus microplus following vaccination with recombinant glutathione s-tra ...201729056423
epidemiological survey of ticks and tick-borne pathogens in pet dogs in south-eastern china.to understand the epidemiology of tick infestation and tick-borne diseases in pet dogs in south-eastern china and to develop a reference for their prevention and treatment, we collected 1550 ticks parasitizing 562 dogs in 122 veterinary clinics from 20 cities of south-eastern china. dogs were tested for common tick-borne pathogens; collected ticks were identified and processed for the detection of tick-borne pathogens. the use of an in vitro elisa diagnostic kit for antibody detection (snap®4dx® ...201728971797
the efficacies of 5 insecticides against hard ticks hyalomma asiaticum, haemaphysalis longicornis and rhipicephalus sanguineus.at present, chemical-based tick control strategies are still the most efficient and widely used methods in control of ticks and tick-borne diseases. in this study, the efficacies of lambda-cyhalothrin, beta-cypermethrin, emamectin benzoate, spirotetramat and hexaflumuron in vitro were evaluated against hyalomma asiaticum, haemaphysalis longicornis and rhipicephalus sanguineus that are widespread and able to transmit a variety of human and animal diseases in china. the results showed that the lc ...201526115939
identification and characterization of micrornas by deep-sequencing in hyalomma anatolicum anatolicum (acari: ixodidae) ticks.hyalomma anatolicum anatolicum (h.a. anatolicum) (acari: ixodidae) ticks are globally distributed ectoparasites with veterinary and medical importance. these ticks not only weaken animals by sucking their blood but also transmit different species of parasitic protozoans. multiple factors influence these parasitic infections including mirnas, which are non-coding, small regulatory rna molecules essential for the complex life cycle of parasites. to identify and characterize mirnas in h.a. anatolic ...201525592818
[investigation of ticks parasitizing on bodies of dogs in shanghai].to investigate the ticks which parasitize on the bodies of dogs in shanghai.201223373258
morphological, biological and molecular characteristics of bisexual and parthenogenetic haemaphysalis longicornis.the reproductive mechanism of haemaphysalis longicornis is quite different from many other animal species. in this article, several characteristics of parthenogenetic and bisexual populations of h. longicornis were analyzed, including some important micro-structures, synchronized life cycle feature and sequences of mitochondrial 16s rrna gene. the results suggested even though many observations of the two populations were similar to each other, some important differences also existed. the genita ...201222560314
temporal and spatial distribution and species diversity of hard ticks (acari: ixodidae) in the eastern region of caspian sea.ticks are important parasites because of their voracious blood-feeding activity, and being as vectors for various agents of diseases in both human and livestock. this study was conducted in a monthly schedule from october 2014 to december 2015, at 45 study sites in three regions bordering the caspian sea, according to the topography including hillside, plain and coastal areas in collecting ticks from the body of sheep. alfa and beta biodiversity indices were calculated and compared between the t ...201627519473
ectoparasites of hedgehogs (erinaceus concolor) from turkey.hedgehogs are small, nocturnal, spiny-coated animals that have been growing in popularity as exotic pets. however, these animals are host to a wide variety of viruses, bacteria, fungi and parasites, some of which are of zoonotic character. thus, because hedgehogs have a potential role to transmit zoonoses including arthropod-borne diseases, we examined them for their ectoparasites. the study was carried out on hedgehogs found dead mainly due to road casualties in the bursa province of turkey. th ...201526281445
synopsis of the hard ticks (acari: ixodidae) of romania with update on host associations and geographical distribution.the current paper is a synoptic review of the distribution and host associations of the 25 species of hard tick fauna (family ixodidae) in romania. in addition to a full literature survey, original data is presented, based on eight years of occasional or targeted sample collection. the literature data on geographical distribution was transposed digitally to the decimal degree coordinate system. for each species, an updated distribution map is given together with all historical data and new host ...201222544174
prevalence of tick infestation (rhipicephalus sanguineus and hyalomma anatolicum anatolicum) in dogs in punjab, pakistan.this study was conducted to identify ticks that infest dogs in the rawalpindi region of punjab, pakistan. a total of 525 dogs were examined between june and september 2010. of these, 60 (11.42%) were infested with ticks. the morphological features of the ticks were identified by using a stereomicroscope to confirm their identity using morphological keys. two species were observed, namely: rhipicephalus sanguineus and hyalomma anatolicum anatolicum. the prevalence rate of these species was 98.33% ...201322485006
species composition, distribution, ecological preference and host association of ticks in cyprus.tick population and species depend on the effect of biotic and abiotic factors, especially vegetation, climate and host density; cyprus, due to the mild climate, favors the appearance and spread of tick-borne infections. our objective was to identify the tick species present in the island, to investigate their geographical distribution and their epidemiological implications. during a three-year study (2004-2006) we collected ticks from domestic and wild animals over the island of cyprus. data on ...201627822784
potential risk posed by the importation of ticks into the uk on animals: records from the tick surveillance scheme.in order to monitor important tick vectors in the uk, public health england's tick surveillance scheme (tss) receives specimens from across the country for identification. in recent years, an increasing number of these specimens have been removed from animals with a recent history of travel outside the uk. this paper presents all data collated by the tss on ticks entering the country on recently travelled or imported animals since surveillance commenced in 2005. ten different tick species repres ...201829217768
the effect of excluding ungulates on the abundance of ixodid ticks on wild rabbit (oryctolagus cuniculus).tick abundance depends heavily on host density, so reducing access to the host should reduce tick populations in a determined area. in this study we compared the parasitic index (pi = average number of ticks per rabbit) of two wild rabbit populations separated 16 years ago by a fence keeping ungulates from moving freely. two areas were selected (closed and open) wherein 50 wild rabbits per area were sampled for ticks. the pi in the open area (pi = 989.62) was significantly higher than in the clo ...201728840404
ticks and bacterial tick-borne pathogens in piemonte region, northwest italy.a molecular screening for tick-borne pathogens was carried out in engorged and in questing ticks collected in verbano cusio ossola county, piemonte region, italy. engorged ticks were removed from wild and domestic animal hosts. the most abundant and common tick species in the area was ixodes ricinus (192 adults, 907 nymphs). few individuals of ixodes hexagonus (15) and rhipicephalus sanguineus (7) were found among the ticks removed from domestic animals (46 examined ticks). the presence of ricke ...201729189951
reprolysin metalloproteases from ixodes persulcatus, rhipicephalus sanguineus and rhipicephalus microplus ticks.metalloproteases (mps) have been considered essential for blood feeding and other physiological functions in several hematophagous animals, including ticks. we report the characterization of mp sequences of three important ticks from asia, africa and america: ixodes persulcatus (ip-mps), rhipicephalus sanguineus (rs-mps) and r. microplus (brrm-mps). amino acid sequence identity between r. microplus and r. sanguineus mps ranged from 76 to 100 %, and identities among i. persulcatus, i. ricinus and ...201424687173
genetic characterization of rhipicephalus sanguineus (sensu lato) ticks from dogs in portugal.the taxonomic status of the brown dog tick rhipicephalus sanguineus (sensu stricto) is a subject of on-going debate; there is a consensus that populations of this tick species should be referred to as r. sanguineus (sensu lato) until its taxonomic status is resolved. recent genetic studies revealed the existence of more than one lineage of r. sanguineus (s.l.) in temperate countries. in this study, we assessed the genetic identity of ticks collected from rural dogs living in several areas locate ...201728285602
tick vectors of cercopithifilaria bainae in dogs: rhipicephalus sanguineus sensu lato versus ixodes ricinus.recently, dermal microfilariae of a cercopithifilaria species (spirurida, onchocercidae), namely cercopithifilaria bainae , were detected in dogs from several geographical areas of the mediterranean basin. evidence from both laboratory and field studies support the role of the brown dog tick, rhipicephalus sanguineus sensu lato, as an intermediate host of this nematode. in the present study, we investigated the competence of ixodes ricinus nymphs as vectors of c. bainae. on november 2012, fully ...201323771741
deltamethrin as inductor agent of precocious ovarian degeneration in rhipicephalus sanguineus s.l. (acari: ixodidae) ticks.the cosmopolitan species rhipicephalus sanguineus s.l. is one of the most widely distributed ticks all over the world. these ectoparasites are vectors of several pathogens and cause significant direct damage to their hosts. the biological success of these ectoparasites has been attributed to their ovaries and salivary glands, organs that ensure their survival in various environmental conditions. the importance of the ovaries in ticks is that, after mating, the individuals are able to lay approxi ...201728597314
comparative analysis of spermatids of rhipicephalus sanguineus sensu lato (ixodidae) and ornithodoros rostratus ticks (argasidae): morphophysiology aimed at systematics.the phylogenetic relationships among tick species (acari: ixodida) have been revisited by several researchers over the last decades. two subfamilies, rhipicephalinae (ixodidae) and ornithodorinae (argasidae), deserve special attention. the male reproductive system morphology, as well as the ultrastructure of the germ cells, may provide important information for phylogeny and systematics of metazoan groups, with spermatozoa exhibiting characters that can be used for this purpose. with that inform ...201626481487
comparative analysis of mitochondrial markers from four species of rhipicephalus (acari: ixodidae).mitochondrial sequences of four mitochondrial markers: 12s rrna, 16s rrna, cytochrome c oxidase subunit i (cox1) and cytochrome b (cytb) from four rhipicephalus species were analyzed to establish genetic relationships and enable molecular identification. field-collected samples from the species rhipicephalus annulatus, rhipicephalus bursa, rhipicephalus sanguineus and rhipicephalus turanicus were amplified by pcr and compared with genbank™ annotated sequences. pcr products were obtained using pr ...201324091196
comparative analyses of mitochondrial and nuclear genetic markers for the molecular identification of rhipicephalus spp.the genus rhipicephalus (acari: ixodidae) comprises a large number of vectors of pathogens of substantial medical and veterinary concern; however, species identification based solely on morphological features is often challenging. in the present study, genetic distance within selected rhipicephalus species (i.e., rhipicephalus bursa, rhipicephalus guilhoni, rhipicephalus muhsamae, rhipicephalus sanguineus sensu lato and rhipicephalus turanicus), were investigated based on molecular and phylogene ...201324103336
genetic analysis of rhipicephalus sanguineus sensu lato ticks parasites of dogs in africa north of the sahara based on mitochondrial dna sequences.the aim of this work was to determine the evolutionary relationship among tick populations of rhipicephalus sanguineus sensu lato distributed in africa north of the sahara and different lineages of r. sanguineus s.l. distributed in different regions of sub-saharan africa, america and europe through the analysis of dna sequences of two mitochondrial genes. one hundred and thirty six 16s rrna gene sequences and twenty-seven 12s rrna gene sequences of r. sanguineus s.l. were analyzed. phylogenetic ...201728495189
new molecular data shed light on the global phylogeny and species limits of the rhipicephalus sanguineus complex.the rhipicephalus sanguineus complex is a group of closely related tick species distributed all around the world. in this study, using mitochondrial 16s ribosomal dna, new specimens of r sanguineus sensu lato from turkey and rhipicephalus camicasi from kenya, were evaluated together with available sequences of this complex in genbank. our objectives were to delimit the complex, re-evaluate its global phylogeny and develop a reconstruction of its biogeographic history. given turkey's geographical ...201627050755
detection of leishmania (v) guyanensis in rhipicephalus (boophilus) microplus (acari: ixodidae) collected from pecari tajacu.previous studies identified the presence of leishmania infantum in rhipicephalus sanguineus and indicated the possibility that it could transmit leishmaniasis to a variety of hosts.201729161493
synergism of thymol, carvacrol and eugenol in larvae of the cattle tick, rhipicephalus microplus, and brown dog tick, rhipicephalus sanguineus.the effects of combinations of the monoterpenes thymol and carvacrol and the phenylpropanoid eugenol in larvae of rhipicephalus microplus (canestrini, 1888) (acari: ixodidae) and rhipicephalus sanguineus sensu lato (s.l.) (acari: ixodidae) were assessed by the larval packet test. the compusyn program was used to make qualitative assessments of the effects (synergistic, additive and antagonistic) of the associations. the effects of all combinations tested against r. microplus larvae were synergis ...201627392803
high efficacy of a 20 amino acid peptide of the acidic ribosomal protein p0 against the cattle tick, rhipicephalus microplus.current strategies to control cattle ticks use integrated control programs (icp) that include vaccination. reduction in the use of chemicals and in the cost of tick control, the delay or elimination of acaricide resistance and the decreasing of environmental pollution are the advantages of using these programs. this integrated program is potentially applicable to all genotypes of chemical resistant ticks. however, the problem here is to improve the efficacy of anti-tick vaccines. the p0 protein ...201525958782
effect of calea serrata less. n-hexane extract on acetylcholinesterase of larvae ticks and brain wistar rats.acetylcholinesterase (ache), an enzyme that hydrolyses acetylcholine (ach) at cholinergic synapses, is a target for pesticides and its inhibition by organophosphates leads to paralysis and death of arthropods. it has been demonstrated that the n-hexane extract of calea serrata had acaricidal activity against larvae of rhipicephalus (boophilus) microplus and rhipicephalus sanguineus. the aim of the present study was to understand the mechanism of the acaricidal action of c. serrata n-hexane extra ...201222608349
transmission of leishmania infantum by rhipicephalus sanguineus (acari: ixodidae) in dogs.rhipicephalus sanguineus is the most widely distributed tick in the world, which is partly due to its biological flexibility and the global distribution of its major host, the domestic dog. in mediterranean region it could be principal reservoir host for leishmania infantum, usually transmitted by the phlebotomine sand flies. in this study, we evaluated the vector potential of r. sanguineus in transmitting l. infantum to uninfected dogs.201829317872
rhipicephalus sanguineus in an imported dog. 201829305477
molecular evidence for transstadial transmission of ehrlichia canis by rhipicephalus sanguineus sensu lato under field conditions.this study investigated possible transstadial transmission of ehrlichia canis by rhipicephalus sanguineus sensu lato collected from shelter dogs and the shelter grounds in diyarbakır province of south-eastern turkey. totally 225 engorged nymphs were collected from eight infected dogs with e. canis and incubated at 28°c for moulting. unfed ticks from the shelter grounds comprising 1,800 larvae, 3,100 nymphs, and 85 adults were sorted according to sampling origin, life stage, and sex into 116 pool ...201729272463
a molecular survey of rickettsias in shelter dogs and distribution of rhipicephalus sanguineus (acari: ixodidae) sensu lato in southeast turkey.canine tick-borne pathogens are the source of emerging diseases and have important zoonotic relevance. dogs play a major role in the transmission of several zoonotic tick-borne pathogens, as reservoirs and/or sentinels. to simultaneously detect anaplasma and ehrlichia species, a reverse line blot assay was conducted on 219 blood samples collected from autochthonous asymptomatic shelter dogs. one hundred and three (47.0%, ci 40.3-53.9) dogs were positive for one or both rickettsial pathogens. sev ...201729186493
in-vitro trials to ascertain sustained release efficacy of assembly pheromone micro particles for the control of brown dog tick, rhipicephalus sanguineus.sustained release micro particles were prepared incorporating assembly pheromone and deltamethrin. two natural polymers, namely, chitosan and calcium alginate and a synthetic polymer, poly-ε-caprolactone were used for encapsulating the assembly pheromone-acaricide combination. the micro particles were subjected to in vitro evaluation freshly after preparation and then at monthly intervals to assess their sustained release efficacy. the response of the unfed stages of dog tick, rhipicephalus sang ...201729114155
first detection of rickettsia conorii ssp. caspia in rhipicephalus sanguineus in zambia.ticks are important vectors for rickettsia spp. of the spotted fever group all around the world. rickettsia conorii is the etiological agent of boutonneuse fever in the mediterranean region and africa. tick identification was based on morphological features and further characterized using the 16s rrna gene. the ticks were individually tested using pan-rickettsia real-time-pcr for screening, and 23s-5s intergenic spacer region, 16s rdna, glta, sca4, ompb, and ompa genes were used to analyze the r ...201728986641
characterization of a sodium channel mutation in permethrin-resistant rhipicephalus sanguineus (acari: ixodidae).the brown dog tick, rhipicephalus sanguineus (latrielle) sensu lato, is an important ectoparasite of dogs and occasionally humans, capable of transmitting several pathogens, such as rickettsia and ehrlichia, which are of veterinary and medical importance. the brown dog tick is distributed worldwide and has an affinity for human habitations in much of its range. in some populations, lack of integrated pest management plans and overuse of pyrethroid pesticides and other sodium channel inhibitors h ...201728981814
mutation in the sodium channel gene corresponds with phenotypic resistance of rhipicephalus sanguineus sensu lato (acari: ixodidae) to pyrethroids.the brown dog tick, rhipicephalus sanguineus sensu lato (latreille), is a cosmopolitan ectoparasite and vector of pathogens that kill humans and animals. pyrethroids represent a class of synthetic acaricides that have been used intensely to try to control the brown dog tick and mitigate the risk of tick-borne disease transmission. however, acaricide resistance is an emerging problem in the management of the brown dog tick. understanding the mechanism of resistance to acaricides, including pyreth ...201728981687
water immersion of dogs close to the time of topical fluralaner treatment does not reduce efficacy against a subsequent experimental challenge with rhipicephalus sanguineus (sensu lato).fluralaner is a novel systemic ectoparasiticide for dogs and cats providing immediate and persistent flea- and tick-control after a single topical dose. prescribing directions recommend waiting 72 h following topical administration before immersing dogs in water. the objective of this study was to determine whether water immersion immediately prior to treatment or earlier than 72 h post-treatment reduced subsequent treatment efficacy.201728946900
molecular detection and phylogenetic analysis of ehrlichia canis in a philippine dog.canine monocytic ehrlichiosis (cme), caused by a rickettsial bacterium, ehrlichia canis, is distributed worldwide, particularly in tropical and subtropical regions. transmission of e. canis is primarily mediated by the vector tick, rhipicephalus sanguineus sensu lato and the bacteria then infect and replicate in monocytes and macrophages. many cases are seen in veterinary hospitals and treated routinely; however, the genetic variation of e. canis strains found in the philippines has been poorly ...201728943246
bacterial microbiota associated with rhipicephalus sanguineus (s.l.) ticks from france, senegal and arizona.ticks of the group rhipicephalus sanguineus (sensu lato) are distributed worldwide and are major pathogen vectors of both dogs and humans. previous phylogenetic reconstructions have suggested the existence of two main lineages within this group, "tropical" and "temperate". symbiotic interactions contribute to vector development, survival, reproduction and competence. the diversity of microbial communities associated with different populations of r. sanguineus (s.l.) remains poorly characterized, ...201728886749
evaluation of the acaricidal activity of thymol incorporated in two formulations for topical use against immature stages of rhipicephalus sanguineus sensu lato (latreille, 1806) (acari: ixodidae).the objective of this study was to assess, for the first time, the in vitro acaricidal activity of two topical formulations containing thymol, on immature stages of rhipicephalus sanguineus sensu lato. for this purpose, two base formulations were prepared: an oil-in-water (o/w) emulsion and a hydroalcoholic solution, containing different thymol concentrations (0.5 to 20 mg/ml). we used the larval packet test for non-engorged larvae and nymphs, and the immersion test for engorged larvae and nymph ...201728875304
interaction between saliva's adenosine and tick parasitism: effects on feeding and reproduction.it has recently been demonstrated that saliva from rhipicephalus sanguineus ticks contains adenosine (ado) and prostaglandin e2 (pge2), two non-protein molecules that have significant immunomodulatory properties. these molecules can inhibit cytokine production by dendritic cells (dcs), while also reducing the expression of cd40 in these cells. however, more studies are needed for a better understanding of their participation in the feeding of ticks in vivo. this work, therefore, evaluated the im ...201728693553
determination of metabolic resistance mechanisms in pyrethroid-resistant and fipronil-tolerant brown dog ticks.rhipicephalus sanguineus (latreille) (ixodida: ixodidae) is a three-host dog tick found worldwide that is able to complete its' entire lifecycle indoors. options for the management of r. sanguineus are limited and its' control relies largely on only a few acaricidal active ingredients. previous studies have confirmed permethrin resistance and fipronil tolerance in r. sanguineus populations, commonly conferred by metabolic detoxification or target site mutations. herein, five strains of permethri ...201728639697
human feet bitten by multiple brown dog ticks, rhipicephalus sanguineus. 201728529887
cercopithifilaria bainae in rhipicephalus sanguineus sensu lato ticks from dogs in brazil.rhipicephalus sanguineus sensu lato (s.l.) ticks act as intermediate host for a range of canine vector-borne pathogens, including nematodes ranked in the genus cercopithifilaria. though being the object of several studies in the last years, information on the distribution of these parasites is still lacking. in this study, the occurrence of cercopithifilaria spp. was investigated in on-host population of r. sanguineus s.l. collected from naturally infested dogs. ticks (n=1906, including one larv ...201728442240
ultrastructural morphology and molecular analyses of tropical and temperate "species" of rhipicephalus sanguineus sensu lato (acari: ixodidae) in brazil.the rhipicephalus sanguineus (latreille) complex (acari:ixodidae) is composed of species with intra- and interspecific morphological variation that make their diagnosis difficult. in the present study, male specimens of the r. sanguineus complex were collected from dogs in six districts of three regions of brazil and submitted to molecular and scanning electron microscopy (sem) analyses. analysis of cox1 gene, 12s rdna, and d-loop rdna shows that ticks classified as r. sanguineus form two differ ...201728399274
larvae of ixodiphagus wasps (hymenoptera: encyrtidae) in rhipicephalus sanguineus sensu lato ticks (acari: ixodidae) from brazil.the biological control of ticks represents an alternative method to the chemical control, given its ecological-friendly approach. amongst the alternatives, the use of parasitoids of the genus ixodiphagus (hymenoptera: encyrtidae) has been largely investigated. the aim of this study was to document and molecularly characterize ixodiphagus wasps in ticks from a tropical region of brazil. from october 2015 to march 2016, rhipicephalus sanguineus sensu lato ticks (n=1814) were collected from natural ...201728344035
brown dog tick, rhipicephalus sanguineus sensu lato, infestation of susceptible dog hosts is reduced by slow release of semiochemicals from a less susceptible host.domestic dog breeds are hosts for the brown dog tick, rhipicephalus sanguineus sensu lato, but infestation levels vary among breeds. beagles are less susceptible to tick infestations than english cocker spaniels due to enhanced production of 2-hexanone and benzaldehyde that act as volatile tick repellents. we report the use of prototype slow-release formulations of these compounds to reduce the burden of r. sanguineus s. l. on english cocker spaniel dogs. twelve dogs were randomly assigned to tw ...201728340941
using plastic tips in artificial feeding of rhipicephalus sanguineus sensu lato (acari: ixodidae) females.this study evaluated the influence of the initial weight, feeding period and temperature on weight gain and biological parameters of the non-parasitic phase of partially engorged rhipicephalus sanguineus females that were artificially fed using plastic tips as feeding devices. the device did not alter the oviposition of the females or any other parameters evaluated. furthermore, it was observed that the temperature of the feeding the group did not affect the weight gain and biology of ticks. thi ...201728327880
a novel assembly pheromone trap for tick control in dog kennels.a novel ecofriendly sticky tick trap device for the control of dog tick rhipicephalus sanguineus using gold nanoparticle assembly pheromone complex as a bait was developed. assembly pheromones comprising of guanine, xanthine and adenine in the ratio of 25:1:1 was encapsulated in gold nanoparticle. the response of the different stages of unfed r. sanguineus ticks was evaluated using petridish bioassay. statistical analysis was done using chi-square test. petridish bioassay with unfed stages of r. ...201728215869
mitochondrial dna analysis of rhipicephalus sanguineus s.l. from the western iberian peninsula.rhipicephalus sanguineus latreille (1806) (ixodida: ixodidae) is considered to be the most widely distributed tick and to have a vast range of habitats and hosts, including livestock, pets and wildlife. in addition to morphological differences, recent investigations using approaches based on molecular genetic markers have revealed the existence of different r. sanguineus lineages in different geographic regions. in this study, 475 ticks collected from dogs in the western iberian peninsula were s ...201728165138
rhipicephalus sanguineus ticks: a new vector from the continent to the uk? 201728154217
first documentation of ivermectin resistance in rhipicephalus sanguineus sensu lato (acari: ixodidae).the brown dog tick, rhipicephalus sanguineus sensu lato (latreille, 1806), is an ectoparasite and disease vector of significant veterinary and public health importance that is distributed widely around the world. the intensive use of synthetic acaricides for tick control exerts a strong selective pressure for brown dog ticks to become resistant to them. here, we investigated claims from the field regarding treatment failure associated with the use of veterinary products containing ivermectin (iv ...201728043394
rhipicephalus sanguineus importation into the uk: surveillance, risk, public health awareness and one health response.as part of public health england's assessment of vectorborne disease risk to public health in the uk, tick specimens are regularly submitted by veterinarians for identification via the tick surveillance scheme. recently, a number of these specimens have been identified as the brown dog tick, rhipicephalus sanguineus this species is non-endemic to the uk and presents a risk to both human and animal health due to its role in the transmission of various tickborne pathogens. although current climati ...201727999153
rhipicephalus sanguineus (acari: ixodidae) species complex: morphometric and ultrastructural analyses.although some studies have already been performed on rhipicephalus sanguineus (latreille) populations, there is still much disarray around its taxonomic classification. rhipicephalus sanguineus, which is also known as " (brown dog tick) is a three-host tick that parasitizes many vertebrates, including occasional human hosts. it presents a worldwide distribution and is a vector of zoonosis with acknowledged public health importance. in western europe, r. turanicus (pomerantsev) share many phenoty ...201627812830
comparative biology of the tropical and temperate species of rhipicephalus sanguineus sensu lato (acari: ixodidae) under different laboratory conditions.recent studies have shown that the taxon rhipicephalus sanguineus sensu lato (s.l.) is represented in latin america by two distinct species, designated as 'tropical species' (distributed from mexico to brazil) and 'temperate species' (restricted to the southern cone of south america). since both tropical and temperate species of r. sanguineus s.l. are parasites primarily of domestic dogs, the reasons for their distinct geographical distribution in south america could be related to particular req ...201727793613
rickettsia species in human-parasitizing ticks in greece.ticks serve as vectors and reservoirs for a variety of bacterial, viral and protozoan pathogens affecting humans and animals. unusual increased tick aggressiveness was observed in 2008-2009 in northeastern greece. the aim of the study was to check ticks removed from persons during 2009 for infection with rickettsia species.201627198214
identification of leishmania spp. promastigotes in the intestines, ovaries, and salivary glands of rhipicephalus sanguineus actively infesting dogs.sand flies are recognized as the major vector of canine visceral leishmaniasis. however, in some areas of brazil where sand flies do not occur, this disease is found in humans and dogs. there has been speculation that ticks might play a role in transmission of canine visceral leishmaniasis and the dna of leishmania spp. has been reported in whole ticks. we investigated the presence of leishmania spp. promastigotes in the intestines, ovaries, and salivary glands of rhipicephalus sanguineus ticks ...201627169724
phylogeography of rhipicephalus sanguineus sensu lato and its relationships with climatic factors.brown dog ticks morphologically identifiable as rhipicephalus sanguineus sensu lato, are distributed world-wide and their systematics is controversial. results of genetic and reproductive compatibility studies of geographically distinct populations of r. sanguineus s.l. indicate that the r. sanguineus complex is paraphyletic. to further elucidate systematic relationships within r. sanguineus s.l. and geographic boundaries of its lineages, we conducted a phylogeographical study of 136 tick specim ...201627003273
transcriptome of larvae representing the rhipicephalus sanguineus complex.rhipicephalus sanguineus sensu lato (ixodida: ixodidae) is possibly the most widespread tick species worldwide, responsible for transmitting several vector-borne pathogens of medical and veterinary importance. here, we explore the transcriptome of r. sanguineus s.l. larvae (putignano strain). we sequenced total rna from r. sanguineus s.l. larvae. a total of 15,566,986 short paired-end reads were de novo-assembled into 33,396 transcripts and then annotated and analyzed. particular attention was p ...201726921517
a brown dog tick (rhipicephalus sanguineus) bite at sea.medical officers (mos) and medical branch ratings (mbrs) must maintain an awareness of the risk of vector-borne diseases among deployed personnel. personnel working in the maritime environment may not expect to be at risk of tick bites, as ticks typically occupy habitats with dense vegetation such as forests or scrub land. however, tick-borne pathogens can cause serious and sometimes fatal disease, and therefore the risk of tick bites and associated diseases should be recognised. we present a ca ...201526867423
establishing the discriminating concentration for permethrin and fipronil resistance in rhipicephalus sanguineus (latreille) (acari: ixodidae), the brown dog tick.rhipicephalus sanguineus (latreille), the brown dog tick, is a veterinary canine and urban pest. these ticks have been found to develop resistance and tolerance to the two commonly used acaricides permethrin and fipronil respectively. we have developed a discriminating concentration that can be used for rapid detection of permethrin and fipronil resistance in brown dog tick populations. the availability of a discriminating concentration for the brown dog tick provides an inexpensive and rapid re ...201626449370
detection of permethrin resistance and fipronil tolerance in rhipicephalus sanguineus (acari: ixodidae) in the united states.permethrin is a commonly used acaricide for tick control on domestic animals and in residential environments, while fipronil use is restricted to on-animal treatment. following widespread reports of permethrin and fipronil application failures to control indoor infestations of rhipicephalus sanguineus (latreille), the brown dog tick, 31 tick populations were obtained from florida and texas for acaricide resistance screening. these field-collected ticks from kennels and residential facilities wer ...201526334817
overwintering of the brown dog tick in residential properties in england--raising awareness. 201526251540
lack of acquired resistance in dogs to successive infestations of rhipicephalus sanguineus ticks from brazil and argentina.comparative studies between brown dog tick rhipicephalus sanguineus populations from brazil (jaboticabal, são paulo) and argentina (rafaela, santa fé) showed significant biological, morphological and genetic differences between them. this work aimed to study, in a comparative way, the acquisition of resistance in domestic dogs to r. sanguineus from jaboticabal and rafaela, after successive and controlled infestations. ticks were kept in a bod incubator under controlled conditions (27 °c, 80 % re ...201526063405
brown dog tick infestation of a home in england. 201525634925
the taxonomic status of rhipicephalus sanguineus (latreille, 1806).the brown dog tick, rhipicephalus sanguineus sensu stricto, is a species with considerable public health and economic importance. however, the taxonomic status of this species is far from resolved. after more than 110 years of scientific work on r. sanguineus s.s., the situation is that there is no type, no solid description, nor is there a consensus about the range of morphological variability within the species. recent findings based on laboratory crosses and molecular genetics strongly sugges ...201525560924
potential role of atp-binding cassette transporters against acaricides in the brown dog tick rhipicephalus sanguineus sensu lato.atp-binding cassette (abc) transporters have been shown to be involved in pesticide detoxification in arthropod vectors and are thought to contribute to the development of drug resistance. little is currently known about the role they play in ticks, which are among the more important vectors of human and animal pathogens. here, the role of abc transporters in the transport of fipronil and ivermectin acaricides in the tick rhipicephalus sanguineus (ixodida: ixodidae) was investigated. larvae were ...201525530472
histopathological study of ovaries of rhipicephalus sanguineus (acari: ixodidae) exposed to different thymol concentrations.thymol is a monoterpene with proven acaricide action for several tick species. in addition to killing these ectoparasites, thymol can also reduce oviposition and egg hatch rate. however, the effects of thymol on the morphophysiology of tick ovaries are still unknown. thus, the aim of this study was to evaluate the morphophysiological changes caused by this active principle in ovaries of rhipicephalus sanguineus after a 6-day feeding period, through the application of morphohistochemical techniqu ...201425300417
first report of cercopithifilaria spp. in dogs from eastern europe with an overview of their geographic distribution in europe.dogs in europe may be parasitized by a variety of species of filarioids. while some species have been studied for decades, others have been only recently reported, and their range of distribution is still unknown. the present study was aimed to investigate the occurrence of cercopithifilaria spp., whose microfilariae reside in the dermis and are transmitted by rhipicephalus sanguineus s.l. ticks, in a dog population from romania and to present the current knowledge of their distribution in europ ...201424825312
new method using quantitative pcr to follow the tick blood meal and to assess the anti-feeding effect of topical acaricide against rhipicephalus sanguineus on dogs.a 28-day study was conducted to assess the dynamic of blood feeding by rhipicephalus sanguineus ticks on dogs treated or not with a novel topical combination of fipronil, amitraz and (s)-methoprene. dogs were infested weekly through exposure to ticks in crates for 4h. ticks were then counted in the crates at 2h and 4h post dog exposure. ticks were also counted and removed from the dogs at 2h, 4h, 6h, 12h and 24h post tick exposure. the inhibition of blood feeding was assessed by both tick quanti ...201424656063
Displaying items 1601 - 1700 of 1724