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in vitro detection of immune responses to mtv-induced mammary tumors: activity of spleen cell preparations from both mtv-free and mtv-infected mice. 19744360546
cell culture techniques in the search for cancer viruses of man. 19714360734
c-type oncogenic viruses of nonhuman primates. 19744360745
sites of synthesis of murine rna tumor virus (oncornavirus) group-specific antigens. 19744360841
dexamethasone stimulation of murine mammary tumor virus expression: a tissue culture source of virus.in mouse cell lines derived from mammary adenocarcinomnas, the synthetic steroid dexamnethasone stimulates production of murine mammary tumor virus. viral rna and antigens are increased as much as 20-fold, and culture fluid supernatants from steroid-treated cells contain type b particles with reverse transcriptase. these cells provide a possible tissue culture source of this virus and a model system for studying the mechanism of action of corticosteroids and the regulation of transcription of in ...19744361099
[regulation of immunologic tumor-host relations in virus-induced mammary carcinoma of the mouse]. 19734361625
membrane alterations which accompany mumtv maturation. i. studies by freeze-cleave techniques. 19744362027
glucocorticoid receptors in mouse mammary tumors. specific binding of glucocorticoids in the cytoplasm. 19744362062
[location of viral antigens in mouse mammary adenocarcinoma by immunoperoxidase]. 19734362169
[immunofluorescence and the immunoelectron microscopy of virus-induced tumors of the mammry glands of cba-bln strain mice]. 19734362195
the polypeptide composition of mouse mammary tumor virus. 19744362427
immunotherapeutic studies in mice with the methanol-extraction residue (mer) fraction of bcg: solid tumors. 19734362523
life cycle of the murine mammary tumor virus, and a comparison with similar forms reported in human mammary carcinomas. 19734362650
effect of murine milk samples and human breast tissues on human leukocyte migration indices. 19744363063
[the effect of thymectomy, irradiation and immunization of c3h-he line mice on the development of syngenous mammary gland tumors]. 19734363186
[proof of the existence of antigens in virus induced mammary gland tumors of mouse line cba-bln]. 19734363190
separation of b and c type virions by centrifugation in gentle density gradients.murine type b particles were separated from type c (rauscher leukemia virus) by means of gentle (low-increment rate) density gradients. the best separation was obtained when the density ranged from 1.13 to 1.20 g/cm(3) when sucrose was used and from 1.12 to 1.28 g/cm(3) with cscl. the buoyant densities of the b and c particle bands in sucrose were 1.18 and 1.16 g/cm(3), respectively. the cscl gradient gave a better separation with the b particles banding at a density of 1.20 g/cm(3) and with the ...19744363254
antigenic structure of murine mammary tumour viruses. 19734363444
[current views on the viral origin of cancer]. 19744363460
virus oncogenesis and tumor immunogenicity in the mouse mammary tumor system. 19744363654
feline malignant mammary tumors. ii. immunologic and electron microscopic investigations into a possible viral etiology. 19744363687
mammary tumor virus in strain gr mice in relation to age and tissue type. 19744363702
differential effect of ionizing radiation on mammary tumor virus activity associated with blood cells and mammary tumor cells. 19744363707
hemic cell-associated mammary tumor virus activity in balb-cfc3h mice. 19744363708
transmission of mammary tumor virus by the gr mouse strain. i. role of the virus in the production of lesions. 19744363709
blocking antibodies and enhancement. 19724364363
transmission of the mammary tumor virus by the gr mouse strain. ii. genetic studies. 19744364735
balb-cfr3: a new high mammary-tumor and high leukemia mouse strain. 19744364738
mammary tumor virus (mtv) antigens in normal and mammary tumor-bearing mice. 19734364792
a locus affecting circulating interferon levels induced by mouse mammary tumour virus. 19744364881
immunochemical characterization of two major polypeptides from murine mammary tumor virus.a major murine mammary tumor viral (mmtv) antigen, sl, originally described by nowinski et al. (1967, 1968, 1971), has been purified from riii mouse milk mmtv by sequential ion-exchange and gel chromatography. the purified protein with sl antigenic reactivity contains carbohydrate, and has an apparent minimal molecular weight of 52,000. it can be designated as gp52 (sl). another major mmtv viral protein with a molecular weight of 27,000 has also been isolated, and antisera have been prepared aga ...19744364896
titrations of various mouse mammary tumor viruses in different mouse strains. 19744365388
mechanism of resistance to mammary tumor development in c57bl and i strains of mice. ii. inherent differences between the two strains. 19744365389
relationship between hydration and proton nuclear magnetic resonance relaxation times in tissues of tumor-bearing and non-tumor-bearing mice: implications for cancer detection. 19744365678
homology between human breast tumour rna and mouse mammary tumour virus genome. 19744365713
presence and transmission of mammary tumor virus and leukemia virus in the balb-cfriii mouse. 19744365907
lymphocyte inhibition by mammary tumor virus in patients with breast cancer. 19744366357
radioimmunoassay for mouse mammary tumor virus-associated antigen. 19744367180
expression of mammary tumor virus antigen on the membranes of lymphoid cells. 19744367759
possible viral etiology of human cancer. 19734367762
[epithelial culture isolated from t 3 mouse mammary adenocarcinoma: characteristics during 5 years]. 19744368127
an epidemiologic study of breast cancer. 19744369771
editorial: neuroblastoma: possible viral aetiology? 19744369946
of birds and mice and men: comments on the use of animal models and molecular hybridization in the search for human tumor viruses. 19744371153
human cancer and animal viral oncology. 19744371267
immunologic evidence for horizontal transmission of mtv. 19744371310
cation preferences for poly(rc)-oligo(dg)-directed dna synthesis by rna tumor viruses and human milk particulates. 19744371968
immunological relationship between antigens induced by leukemia virus & mammary tumor virus in an inbred mouse strain. 19744372168
distribution of virus particles and mammary tumor virus antigens in mouse mammary tumors, transformed balb-c mouse kidney cells, and gr ascites leukemia cells. 19744372372
histocompatibility genes (the h-2 complex) and susceptibility to mammary tumor virus in mice. 19744372373
carcinogenic effects of oral diethylstilbestrol on c3h male mice with and without the mammary tumor virus. 19744372382
further observations on the production of oncornaviruses by mjy-alpha cell line. 19744372383
structure of the mouse mammary tumor virus: characterization of bald particles.the polypeptide, antigenic, and morphological structure of the mouse mammary tumor virus was studied following protease digestion of intact virions. intact, untreated virions (rho = 1.17 g/ml) had characteristic envelope spikes, five major polypeptides, and were precipitated by antisera against gp52. two of the major polypeptides, with molecular weights of 52,000 (gp52) and 36,000 (gp36), had carbohydrate moieties. protease treatment resulted in spikeless, "bald" particles (rho = 1.14 g/ml), whi ...19744372399
[further investigation to the immunology of murine mammary tumours of the cba-bln strain using the colony inhibition tests (author's transl)]. 19744372968
the role of latent viruses in subline differentiation in inbred strains of mice. 19744373307
detection of mouse mammary tumor virus in cat kidney cells infected with purified b particles from riii milk. 19744373580
tumor-specific antigens and reexpression of fetal antigens in mammalian cells. 19744373784
remarks on the ultrastructure of type a, b, and c virus particles. 19744374071
mechanical milk collection from mice for bittner virus isolation. 19744374602
the treatment of breast cancer. 19744375821
[regulating mechanisms in the tumor-host relation in murine mannary carcinomas]. 19734375940
[correlation between the production of mature mammary tumour virus particles and the histomorphological differentiation of murine mammary tumours].the mammary tumour virus (mtv) contents of mammary tumours (mt's) of various mouse strains were investigated by means of the indirect immunofluorescence technique and the electron microscopy. only in acinus-forming, well differentiated mt's mtv-b particles could be observed in high quantity. the release of b particles seems to be correlated with the degree of structural ripeness of the tumor tissue. as to intracytoplasmic a particles such a strong relationship could not be found out. many a part ...19744376679
proceedings: preparation and partial chemical analysis of milk-fat-globule membrane and mammary tumour virus obtained from swiss albino mice. 19744376764
characterization of x gf mice with respect to their resistance to oncogensis. 19744377184
[the most recent biochemical studies in experimental animal models of breast neoplasms]. 19744377391
studies on the possibility of induction of anti-h-2 alloreactive cytotoxic antibodies by syngeneic immunization.our studies on the syngeneic immunization with normal cells did not confirm the possibility of induction of alloreactive cytotoxic antibodies in this way. we were not able to induce any antibodies after syngeneic immunizations within balb/c and balb/cfc3h strains of mice. anti-h-2 cytotoxic antibodies were raised by syngeneic immunization within balb/mo strain of mice, but it was found that this phenomenon was due to the heterogenicity of this strain.19846085470
type and group specific antigenic reactivity associated with the major membrane protein of murine mammary tumor virus. 19846085503
studies of mammary carcinoma metastasis in a mouse model system. i: derivation and characterization of cells with different metastatic properties during tumour progression in vivo.the biological and metastatic properties of cells from a murine mammary adenocarcinoma, mt1, were studied during serial transplantation in syngeneic hosts. over 35 generations the tumour progressed from a well-differentiated, poorly metastatic neoplasm to an anaplastic highly metastatic state. at early passages the tumour yielded uniform cultures of cuboidal epithelial cells, at passage 17 both epitheloid and spindle type cells were present, and by passage 30 only spindle type cells were obtaine ...19846085722
induction of cloned genes after transfer into eukaryotic cells. 19836086030
development and characterization of the balb/cniv mouse strain.the strain balb/cniv/crgl was developed by infecting balb/c/crgl mice with mouse mammary tumor virus from c3hf mice. a balb/c normal mammary duct was transplanted into the gland-free fat pad of a hormone-stimulated female c3hf x balb/c f1 mouse. a hyperplastic alveolar nodule was found in the balb/c ductal outgrowth and was transplanted into another hybrid gland-free fat pad. the resultant hyperplastic alveolar outgrowth was finally transplanted to female balb/c mice. the hyperplastic alveolar o ...19846088036
reversible and persistent changes in chromatin structure accompany activation of a glucocorticoid-dependent enhancer element.a derivative of mouse mammary tumor virus (mtv) dna, ltl, was constructed in vitro and introduced into the genome of mouse l cells. transcription of ltl was stimulated by dexamethasone, a glucocorticoid hormone. two features of ltl chromatin structure are altered upon hormone treatment. first, "moderate" dnaase i sensitivity of the entire ltl element increases following addition of dexamethasone; this alteration persists after hormone withdrawal, when ltl transcription is shut off. second, a dis ...19846088072
functional role for c-myc in mitogenic response to platelet-derived growth factor.in balb/c-3t3 cells, expression of the c-myc gene is stimulated by platelet-derived growth factor (pdgf). using mouse mammary tumour virus promoter: c-myc recombinant plasmids, 3t3 sublines were constructed in which hydrocortisone was the primary determinant of myc mrna content. the c-myc gene product is an intracellular mediator of the growth response to pdgf though probably not the only one. both the human and the mouse c-myc genes stimulate clonal growth of 3t3 cells in pdgf-free medium sugge ...19846088986
mutagenic effects of dna-containing oncogenic viruses and malignant transformation of mammalian cells.it was discovered in the 1970s that oncogenic viruses could induce gene mutations in mammalian cells. the phenomenon seems to be widespread: it was observed with all groups of dna-containing viruses and some retroviruses. the mutagenic effects of the tested viruses at gene level are not locus specific. the viruses induce point mutations, including base substitutions, as well as deletions and insertions. the mutagenic effect of sv40 is controlled by the activity of the early a gene, which encodes ...19846090006
selenium inhibition of the neoplastic transformation in preneoplastic mammary cell populations.the effect of dietary selenium on the tumor-producing capabilities of primary hyperplastic alveolar nodules (han) of the mammary gland of balb/c mice was examined directly using a transplantation assay. the tumor-producing capabilities of primary han exposed to 2.0 ppm dietary selenium was decreased 57% compared to the tumor-producing capabilities of han exposed to 0.2 ppm dietary selenium. this degree of inhibition compared well with the 60% inhibition of mammary tumorigenesis when the mice wer ...19846090012
modulation of enhancer activity by the hormone responsive regulatory element from mouse mammary tumor virus.addition of the transcriptional enhancers present in the u3 region of the harvey murine sarcoma virus (hamusv) long terminal repeat (ltr) to recombinant chimeras in which the hamusv transforming gene (ha-v-ras) is expressed from the mouse mammary tumor virus (mmtv) promoter increases the ability of the mmtv v-ras chimeras to transform mouse fibroblasts in culture 50- to 100-fold. significant stimulation of transfection efficiency occurs only when glucocorticoids are present in the culture medium ...19846090130
progression to steroid autonomy in s115 mouse mammary tumor cells: role of dna methylation.although monoclonal in origin, mammary tumors acquire a marked heterogeneity of cell phenotypes, including a mixture of steroid hormone-sensitive cells and insensitive cells. we describe here long-term studies on the effects of androgen withdrawal on cloned androgen-responsive s115 mouse mammary tumor cells as a model system to investigate mechanisms by which tumor cells lose their steroid sensitivity. in the prolonged absence of androgen, the cells lost hormone-sensitive parameters reproducibly ...19846090471
urethan-induced mammary tumorigenesis in a murine mammary tumor virus (mumtv)-positive mouse strain: evidence for a keratinized nodule as an mumtv-negative precursor lesion for squamous cell tumors.administration of urethan (cas: 51-79-6; carbamic acid, ethyl ester) in the drinking water of breeding female mice of the murine mammary tumor virus (mumtv)-positive dd/tbr strain and the mumtv-negative ddf strain induced so-called keratinized nodules, which were demonstrable in wholemount preparations of mammary glands. it also induced squamous cell tumors (also called adenoacanthoma) at an extremely early age. the keratinized lesions appearing in the lobular areas of the mammary glands showed ...19846090751
hyperplastic and neoplastic changes in the mammary glands of feral mice free of endogenous mouse mammary tumor virus provirus.laboratory colonies of feral mice (mus musculus domesticus) have been established with specific mouse mammary tumor virus (mumtv) genotype, including colonies lacking any proviral dna (ev-) or carrying only a single copy of mumtv dna (ev+). no evidence of a decline in reproductive capacity has been observed in the first 8 generations. both the ev- and ev+ mice showed normal mammary gland development and the development of hyperplastic lesions in the older females. the mice were very resistant to ...19846090752
transgenic mice inherit cancer. 19846090924
the mouse mammary tumor virus model in studies of glucocorticoid regulation. 19846091192
humoral and cellular immune responses to mouse mammary tumor virus (mmtv) in experimental animal models (review).mouse mammary tumor virus (mmtv) has been implicated in the etiology of mouse mammary tumors although genetic, hormonal and environmental factors appear to be involved in the mechanism of carcinogenesis. strains from mice harboring the exogenous mmtv have higher incidences of spontaneous mammary tumors than those who are devoid of the b type particles. however, multiple copies of mmtv provirus are detectable in all inbred strains of mice regardless of whether they develop spontaneous tumors or n ...19846091529
hormonal regulation of the rous sarcoma virus src gene via a heterologous promoter defines a threshold dose for cellular transformation.we have derived rat cell lines producing different and regulatable amounts of pp60v-src by introducing the src gene of rous sarcoma virus (rsv) under the control of the glucocorticoid-responsive transcriptional promoter from the mouse mammary tumor virus (mmtv). we find that the cellular phenotype is strictly dependent upon the dose of pp60v-src with a distinct threshold for changes indicative of neoplastic potential. cells with low constitutive levels of pp60v-src are not phenotypically disting ...19846091899
structure and nucleotide sequence of the putative mammary oncogene int-1; proviral insertions leave the protein-encoding domain intact.many mammary tumors induced by mouse mammary tumor virus (mmtv) contain a provirus in the same region of the host-cell genome, leading to expression of a putative cellular oncogene called int-1. here we present the structure and nucleotide sequence of int-1. we have established several proviral insertion sites exactly by nuclease s1 analysis or by molecular cloning and dna sequencing. the protein-encoding domain of int-1 is distributed over four exons. at the 5' end of the gene two overlapping e ...19846091914
factors affecting spontaneous tumor incidence rates in mice: a literature review.the recent increased use of mice for lifetime oncogenicity testing has resulted in concern for the modulating effects on spontaneous tumor incidence rates by factors other than the test article and the high variability of spontaneous tumor incidence rates in control mice. the various factors shown to affect spontaneous tumor incidence rates in mice must be considered in the planning and conduct of oncogenicity studies with this species if biologically meaningful results are to be achieved. this ...19846091996
isolation and characterization of lymphosarcoma p1798 variants selected for resistance to the cytolytic effects of glucocorticoids in vivo and in culture.clonal subpopulations of lymphosarcoma p1798 have been subjected to glucocorticoid selection in vivo and in culture and the glucocorticoid binding and responsiveness of the resistant variants have been compared with those of the parental lines. cell populations that are resistant to the cytolytic effects of glucocorticoids in vivo exhibit a slightly reduced level of glucocorticoid binding, although nuclear translocation of the hormone-receptor complex is not reduced. sensitive and resistant tumo ...19846092172
genetic changes in mammalian cells reminiscent of an sos response.prior to the isolation of mammalian dna repair genes and identification of their gene products, the comparison between the bacterial sos response and various similar reactions in mammalian cells remains rather speculative. the increasing number of observed phenomena including enhanced dna repair, virus induction, induced cellular differentiation, and neoplastic transformation, all following dna damage or arrest of replication, are, however, suggestive of an sos-like system of growth control and ...19846092260
organization and expression of mouse mammary tumor virus sequences in normal and neoplastic c3hf/hesed mouse tissues.the organization and expression of germinally transmitted mouse mammary tumor virus (mmtv) proviruses in c3hf/hesed mouse tissues were examined. digestion with the restriction enzymes ecori, bamhi, and hindiii and hybridization with cloned probes specific for the long terminal repeat and the 5' and 3' regions of the mmtv genome revealed three full-length (units ib, ii, and v) and two subgenomic (units i and ix) mmtv proviruses in c3hf/hesed mouse germ line dna. the ecori fragments (15.0 and 5.7 ...19846092663
use of the mouse mammary tumor virus long terminal repeat to promote steroid-inducible expression of v-mos.we used the mouse mammary tumor virus long terminal repeat to promote dexamethasone-regulated expression of the moloney murine sarcoma virus (m-msv) transforming gene, v-mos. a recombinant dna vector containing the mouse mammary tumor virus long terminal repeat fused to the m-msv 124 v-mos gene was cotransfected with a plasmid containing the herpes simplex virus thymidine kinase gene (tk) into 3t3tk- cells. individual clones of cells which grew in hypoxanthine-aminopterin-thymidine medium were t ...19846092668
mouse mammary tumor virus-related sequences in mouse lymphocytes are inducible by 12-o-tetradecanoyl phorbol-13-acetate.cdna libraries from el-4 cells treated with 12-o-tetradecanoyl phorbol-13-acetate (tpa) were screened for tpa-inducible sequences by differential hybridization. the most abundant inducible species was a sequence similar to that of mouse mammary tumor virus (mmtv). induction of the mrna corresponding to the mmtv-related sequences was already evident 30 min after tpa treatment, whereas the maximum accumulation occurred after 20 h of exposure to tpa. tpa also increased levels of mmtv-related rna in ...19846092699
integration of type b retroviral dna in virus-induced primary murine thymic lymphomas.in previous studies we described the isolation and characterization of a highly leukemogenic virus, dmba-lv, isolated from a transplanted, chemical carcinogen-induced thymic lymphoma. the virus is composed of a mixture of two unrelated retroviral genomes, one highly related to type b milk-borne mouse mammary tumor virus isolates and the other partially related to type c viral genomes. in the present study, primary thymic lymphomas induced by dmba-lv in cfw/d, nih swiss, c3h/bi/ka, and c57bl/ka m ...19846092711
the methylation pattern of endogenous mouse mammary tumor virus proviral genes is tissue specific and stably inherited.the methylation pattern of mouse mammary tumor virus (mmtv) proviral genes endogenous to the mouse strains c3h, 020, fm/jmsa, c57bl6, and balb/c were investigated in various organs and mammary tumor tissue. digestion of dna with ecori or with ecori and hpaii followed by southern blotting analysis and hybridization to a nick-translated mmtv dna, allowed the distinction between the fully methylated and hypomethylated gene copies. mmtv proviral gene methylation was found to be organ specific, and t ...19846093365
control of gene expression by glucocorticoid hormones.glucocorticoids control the expression of a small number of transcriptionally active genes by increasing or decreasing mrna concentration. either effect can result from a transcriptional or a post-transcriptional mechanism. induction of mouse mammary tumour virus rna results from a stimulation of transcription initiation and depends on the presence of defined regions in proviral dna. these regions bind the glucocorticoid receptor and behave functionally as proto-enhancers. glucocorticoid-inducib ...19846095813
spontaneous and induced metastasis of naturally occurring tumors in mice: analysis of cell shedding into the blood.the lung colonization capability (after iv inoculation) of each of 77 autochthonous virus-induced mammary tumors in c3h/avy female mice was compared with its own spontaneous metastatic behavior. twenty-four of these neoplasms had spontaneously metastasized to the lungs of the tumor bearer. the results were interpreted in the framework of dose-response studies on experimentally induced metastasis. these results indicate that tumors of high lung colonization potential (hcp) overgrow the lungs with ...19846096621
protoneoplasia: the molecular biology of murine mammary hyperplasia.the mouse mammary tumor virus has provided a window into the inner workings of the mammary epithelial cell at the earliest stages of neoplasia. techniques of molecular biology permitted us to look through that window revealing a new biology which deserves consideration as a model for mammary tumorigenesis in all species. according to this model the neoplastic process originates in a single mammary epithelial cell as a result of a critical genetic alteration, such as integration of mumtv (mumhv) ...19846098147
covalent and noncovalent receptor-glucocorticoid complexes preferentially bind to the same regions of the long terminal repeat of murine mammary tumor virus proviral dna.dexamethasone 21-mesylate, an irreversible antiglucocorticoid in htc cells, forms a covalent receptor-steroid complex which can be activated in cell-free systems. the molecular basis of its antiglucocorticoid activity is unknown; it might result from altered dna sequence preferences and/or affinities of the covalent receptor-steroid complex. to test this hypothesis, the affinities of both covalent receptor-antagonist and noncovalent receptor-agonist complexes for defined dna sequences were measu ...19846099146
expression and demethylation of germinally-transmitted balb/c mouse mammary tumor virus dna in abelson mulv b-lymphoid cell lines.murine mammary tumor virus (mmtv) rna expression, dna organization and dna demethylation were examined in balb/c b-lymphoid cell lines produced by transformation with the abelson murine leukemia virus (abmulv). the mmtv dna sequences in abmulv b cell lines, based on restriction mapping with ecori, psti, bglii, bamhi and saci and molecular hybridization with cloned probes of the mmtv ltr, gag-pol or env gene regions, were identical to the germinally-transmitted mmtv dna complement of balb/c mice. ...19846099938
autochthonous humoral immune responses to exogenous and endogenous murine mammary tumor viruses in c3h (jax) and icrc mice. 19846100106
expression of proviral dna of mouse mammary tumour virus and its transcriptional control sequences. 19846101072
isolation of glucocorticoid-unresponsive rat hepatoma cells by fluorescence-activated cell sorting.we have used a mouse mammary tumor virus (mmtv)-infected rat hepatoma cell line as a model system for studying glucocorticoid action. these cells induce tyrosine aminotransferase and mmtv in response to the synthetic glucocorticoid, dexamethasone. the major viral antigen, a glycoprotein of 52,000 daltons (gp52), appears on the surface of infected cells in amounts which reflect the cytoplasmic content of viral rna. using an anti-gp52 antiserum and a fluorescence-activated cell sorter (facs), we h ...19806105925
selection of rat hepatoma cells defective in hormone-regulated production of mouse mammary tumor virus rna.we have been studying the mechanism of glucocorticoid hormone action by using mouse mammary tumor virus (mmtv)-infected rat hepatoma cells as a model system. j2.17, a clonal cell line that contains one mmtv provirus, induces tyrosine aminotransferase (tyratase; l-tyrosine:2-oxoglutarate aminotransferase, ec 2.6.1.5), viral rna, and the cell surface viral glycoprotein gp52 in response to dexamethasone. using a fluorescence-activated cell sorter and a rabbit antiserum directed against gp52, we sel ...19816117075
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