| biochemical and behavioural changes in scrapie. | | 1976 | 782931 |
| platelet inhibition of renal cortical fibrinolytic activity in the rabbit. | a fibrin slide test was utilized to study the effect of platelets and endotoxin on renal cortical fibrinolysis in rabbits. cortical lysis was absent in kidney from animals made markedly thrombocytopenic with goat antirabbit platelet serum or endotoxin; light microscopy was normal and immunofluorescent microscopy for fibrin was negative. no loss of lysis was detected in kidney of animals given goat anti-rabbit albumin or isotonic saline. plasma from animals with endotoxin or antiplatelet antibody ... | 1976 | 785093 |
| characterization of a calf diarrheal coronavirus. | a coronavirus-like agent isolated from feces of a calf with diarrhea and attenuated by consecutive passage in a fetal bovine kidney cell line was characterized as a coronavirus. negatively stained virions were approximately circular, had a mean diameter of 120 nm, and were covered with wide-spaced, petal-shaped projections about 20 nm long. virions in ultrathin sections of infected cell monolayers had a mean diameter of 80 nm, lacked surface projections, and were found within cytoplasmic vesicle ... | 1976 | 786086 |
| [animal losses during air transport]. | | 1976 | 786593 |
| [experimental xenogenic immune pancreatitis. --immunohistological, enzyme histochemical and ultrastructural studies (author's transl)]. | question: repeated intraperitoneal injections of anti-mouse pancreas rabbit serum or of anti-mouse pancreas guinea pig serum produce a chronical sclerotizing pancreatitis. this study has the aim to contribute to the further elucidation of the changes which occur in the acinar cells, as well as to the etiology and pathogenesis of immune pancreatitis, by means of immunohistological, enzyme histochemical and electron microscopic studies. material and methods: anti-mouse pancreas rabbit serum was o ... | 1975 | 786715 |
| caprine pneumonia. ii. biochemical characterisation and serological identification of mycoplasmas. | thirty strains mycoplasma isolated from pneumonic lungs of goats were chracterised biochemically and serologically. on the basis of biochemical characteristics, the strains were divided into three groups. (a) eighteen strains which fermented glucose and mannose, digested serum and reduced tetrazolium, (b) 10 strains which reduced tetrazolium, formed "film and spots" and possessed phosphatase activity and (c) two strains which catabolised arginine only. serologically group a strains were identifi ... | 1976 | 788268 |
| detection of endogenous immunoreactive prolactin in rat mammary epithelial cells during lactation. | the question of whether endogenous prolactin might be detectable in normally lactating rat mammary glands (lmg) was examined by light microscopic immunohistochemistry. in an initial study, sections of lmg were incubated sequentially with i) rabbit anti-rat prolactin (aprl); ii) anti-rabbit gamma globulin: peroxidase conjugate (argammag-per), prepared from goat anti-rabbit gamma globulin and horseradish peroxidase, and iii) 3,3'-diaminobenzidine mixed with h2o2(dab). use of this three-step proced ... | 1976 | 789055 |
| positive end expiratory pressure (peep) and right to left shunting in immature goats. | immature fetal goats were exteriorized by caesarean section and catheters were placed in a pulmonary vein (pv), carotid artery (ca), femoral artery (fa), and the main pulmonary artery (pa). the goats were ventilated at 7.5 and 15 cm h20 positive end expiratory pressure (peep) between control periods at 0 peep. right to left shunting (qs/qt) was calculated for three anatomic sites: lung, foramen ovale, and ductus arteriosus, and for two summation shunts: preductal and total. total qs/qt increased ... | 1976 | 790281 |
| uterine luteolytic hormone: a physiological role for prostaglandin f2alpha. | to summarize luteal function during pregnancy briefly, there are physiological processes initiated by the embryo and/or conceptus in early pregnancy that serve to prolong the life-span of the corpus luteum. some of these processes are well defined, but others remain more obscure. the corpus luteum is maintained in a functional state throughout pregnancy (at least in those species described in this review), even though in several species progesterone production by the corpus luteum is not require ... | 1976 | 790424 |
| localization of transferrin on the surface of the human placenta by electron microscopic immunocytochemistry. | during the course of gestation, a large amount of iron is transferred rapidly and unidirectionally from mother to fetus across the placenta. it has been postulated that one of the first steps involved in placental iron transfer involves binding of the maternal transferrin-iron complex to the surface of the placenta and the subsequent removal of iron and release of transferrin back into the maternal circulation. to determine if transferrin is present on the surface of human placental villi, two d ... | 1976 | 791006 |
| localization of seminal plasma proteinase inhibitors in human spermatozoa as revealed by the indirect immunofluorescence technique. | acidic extracts of washed, ejaculated human spermatozoa contain, besides acrosin, two proteinase inhibitors, a trypsin-chymotrypsin (elastase) inhibitor (husi-i) and a trypsin-acrosin inhibitor (husi-ii). using the indirect immunofluorescence technique these inhibitors could be localized in the spermatozoa. ejaculated spermatozoa were treated with monospecific antibodies raised in rabbits against husi-i and husi-ii, respectively, and with fluorescein-labeled igg from goat directed against the ra ... | 1976 | 791787 |
| insulin of indian sheep & goat pancreatic tissue--isolation and content. | | 1976 | 791858 |
| caprine pneumonia iv: pathogenicity of mycoplasma mycoides subspecies capri and caprine strains of mycoplasma mycoides subsp. mycoides for goats. | | 1976 | 791976 |
| caprine mastitis due to cryptococcus neoformans. | the isolation of cryptococcus neoformans from milk and its demonstration by direct microscopy has been reported in a case of caprine mastitis. this is believed to be the first instance of caprine mastitis in which c. neoformans has been implicated as the etiologic agent. | 1976 | 793054 |
| [antigen spectrum of allergic rhinitis (author's transl)]. | the results of 10.000 individual tests have shown that it is necessary to revise and supplement the influences and the emergence of new antigens support this demand. refined testing for grass pollen seems to be of particular importance, whereby cross reactions must be taken into account. the test program we must, furthermore, take into consideration the geographical incidence distribution of trees and herbs. in austria, a considerable amount of ragweed pollen has recently been ascertained to be ... | 1976 | 793187 |
| visualization principles in thin-layer immunoassays (tia) on plastic surfaces. | macromolecules may adsorb firmly as a monolayer to plastic surfaces and still retain their property to react specifically with antibodies. in the present communication four different principles for visualization of such antigenantibody interactions on plastic surfaces are described. in addition, a diffusion-ingel method for quantitation of antibodies is presented. the described methods offer simple, sensitive and accurate means of assaying antigen-antibody reactions. | 1976 | 793997 |
| acute respiratory, circulatory and pathological changes in the calf after intravenous injections of the galactan from mycoplasma mycoides subsp. mycoides. | twenty of 28 calves, 10-12 weeks of age when given intravenous injections of the galactan from mycoplasma mycoides subsp. mycoides, showed transient apnoea, increased pulmonary arterial and decreased systemic arterial blood pressures, and increased packed-cell volume. necropsy revealed haemorrhages associated with alveolar ducts and vessel walls, areas of pulmonary oedema, usually associated with the haemorrhages, dilated airways and, in some, capillary thrombosis. animals that had shown changes ... | 1976 | 794475 |
| immunity to mycoplasmas causing respiratory diseases in man and animals. | | 1976 | 795287 |
| in vitro effect of thymosin on t-lymphocyte rosette formation in rheumatic diseases. | the in vitro effect of calf thymosin fraction 5 on t-rosette forming cells (e-rfc) was studied in sjögren's syndrome (ss), rheumatoid arthritis (ra), and systemic lupus erythematosus (sle). the baseline percent e-rfc in sixteen normal controls was67-2 +/- 6-9. e-rfc was significantly decreased in sle (42-6 +/- 17-0, p less than 0-0001) and ss (51-8 +/- 16-9, p less than 0-002) but not in ra (59-7 +/- 14-1). ten of twenty-five ss patients and two of eleven ra patients had less than 50% e-rfc, and ... | 1976 | 795579 |
| quantitative studies of peroxidase labeled antibody. i. indirect staining system analyzed by chessboard titraions. | the sensitivity and specificity of immunohistological staining procedures employing horseadish peroxidase labeled antibody were evaluated with the aid of chessboard titrations. two indirect staining systems, detecting antinuclear antibody and pemphigus intercellular antibody, were examined with reference to indirect immunofluorescence. in both systems, chessboard titration results showed that plateau titers of serum antibodies appeared to be a function of label content. the plateau endpoints ref ... | 1976 | 796390 |
| the influence of pyrogen induced fever on absorption of sulpha drugs. | | 1976 | 797098 |
| a comparison of the role of kinins and serotonin in endotoxin induced fever and trypanosoma vivax infections in the goat. | goats were infected with trypanosoma vivax or inoculated with a low pyrogenic dose of lipopolysaccharide (lps) obtained from escherichia coli or salmonella typhimurium in order to obtain evidence about the role of kinins and serotonin in the pathogenesis of trypanosomiasis and in endotoxin induced ruminal stasis. the following conclusions were made: (1) whole blood serotonin and bradykinin-like activity levels and clinical symptoms during fever, induced by lps e coli or t vivax infection are not ... | 1976 | 799824 |
| the physiology of lactation. | | 1976 | 800226 |
| serum igd and ige concentrations in immunodeficiency diseases. | concentrations of igd and ige were measured in sera from 165 patients with well-defined immunodeficiency in an effort to find information possibly relevant to the roles of antibodies of these classes in host defense. values for both immunoglobulins were generally quite low in patients who had marked deficiencies of all three major immunoglobulins, although occasional normal or high normal values for igd were seen in hypogammaglobulinemic patients. group mean igd concentrations were also depresse ... | 1975 | 803218 |
| new immunolatex spheres: visual markers of antigens on lymphocytes for scanning electron microscopy. | new immunochemical reagents consisting of antibodies bound to small latex spheres were used as visual markers for the detection and localization of cell surface antigens by scanning electron microscopy. cross-linked latex spheres of various sizes from 300 to 3,4000 a in diameter were synthesized by aqueous emulsion copolymerization of methacrylate derivatives containing hydroxyl and carboxyl functional groups. proteins and other molecules containing primary amino groups were covalently bonded to ... | 1975 | 803228 |
| lymphocytes bearing b- and t-cell markers in patient with lymphosarcoma cell leukemia. | | 1975 | 803233 |
| rapid diagnosis of venezuelan equine encephalomyelitis by fluorescence microscopy. | goat venezuelan equine encephalomyelitis (vee) antiserum and normal serum were conjugated and evaluated for staining sensitivity and specificity. cross-staining with either eastern or western equine encephalomyelitis virus-infected cells did not occur. the baby hamster kidney (bhk-21) cell line when combined with highly specific vee conjugate detected 100 medium suckling mouse intracerebral lethal doses (suckling mouse ld-50/ic) of the 1b subtype of vee virus per milliliter of equine tissue susp ... | 1975 | 803352 |
| purification from bovine blood of the warfarin-induced precursor of prothrombin. | | 1975 | 803373 |
| immunological cross-reactions of alpha-lactalbumins from different species. | four rabbit antibodies have been prepared, which are specifically directed against alpha-lactalbumins from different sources; namely human, cow, goat and sheep milk. each of these antibodies was tested for its ability to react with, separately, each of the four proteins. the immunological reactions were assessed by means of different techniques: double immunodiffusion in agar gel as well as affinity chromatography of antibodies, using antigens covalently bound to an insoluble matrix. in each cas ... | 1975 | 803446 |
| evaluation of immunological specificity of flurescein-conjugated antisera with agarose-antigen sections. | purified immunoglobulins were conjugated to agarose beads; the beads were frozen, and used to establish the specificity of fluorescein-conjugated antisera. | 1975 | 803474 |
| the distribution of a rabbit thymic antigen and membrane immunoglobulins in lymphoid tissue, with special reference to mucosal lymphocytes. | we have examined rabbit lymphocytes from several tissue sources for membrane immunoglobulins with anti-antiserum for alpha, mu and iota heavy chains, as well as with an antiserum specific for a rabbit thymus lymphocyte antigen (rtla). among lymphocytes from bronchus-associated lymphoid tissue (balt), peyer's patches, thoracic duct and gut mucosa exclusive of peyer's patches, alpha and mu predominated with roughly equal percentages of each being found. the distribution of cells with iota receptor ... | 1975 | 803523 |
| restriction of heterogeneity of goat antibodies specific for human hemoglobin ss. | previously, we had reported the isolation of an antibody population (termed antival antibody) specific for the site of difference between human hemoglobin s(hbs)(beta-6val) and hemoglobin a-1(bba)(beta-6 glu). this population has a stoichiometry of reaction of unity in combining with hbs (alpha-beta-dimer) and shows no reaction with hba. the combination of antival fab fragments with hbs was found to be kinetically homogeneous and had a second order rate constant of 0.58 times 10-6m-1 sec-1 at 20 ... | 1975 | 803525 |
| listeria cell wall fraction: a b cell mitogen. | a crude cell wall-rich fraction of listeria monocytogenes contains a substance or substances which cause blast transformation and thymidine incorporation by mouse spleen cells. the substance, listeria cell wall fraction (lcwf), is a b cell mitogen in the mouse. it is not endotoxin, in that it stimulates b cells in c3h/hej mice. it is weakly mitogenic for rat spleen cells and not at all mitogenic for human peripheral blood or tonsil lymphocytes. thus, it is dissimilar to both endotoxin and purifi ... | 1975 | 803536 |
| comparative effects of strain, species, and sex on the acyltransferase- and sulfotransferase-catalyzed activations of n-hydroxy-n-2-fluorenylacetamide. | the arylhydroxamic acid acyltransferase, an enzyme that promotes the introduction of arylamine groups into nucleic acids, is greater in the stomach, small intestine, colon, and lung of the sprague-dawley rat than in comparable tissues of fischer animals. the enzyme is distributed relatively evenly from the glandular stomach to the distal portion of the colon. no consistent differences in acyltransferase activities of the liver, kidney, brain, or spleen of these two strains were noted. acyltransf ... | 1975 | 803872 |
| two improved methods for preparing ferritin-protein conjugates for electron microscopy. | two improved procedures were developed for activating ferritin so that the ferritin could be covalently linked to antibodies. one procedure involved use of a water-soluble carbodiimide and n-hydroxysuccinimide to prepare ferritin-containing activated esters. the other involved activation of the ferritin with excess glutaraldehyde. the ferritin-antibody conjugates prepared with the two procedures were shown to have a number of properties which made them suitable for locating antigenic components ... | 1975 | 803973 |
| identification and some properties of rat secretory component. | an antiserum, prepared against partially reduced and alkylated rat milk siga, was shown to contain antibodies reacting with a rat milk protein of alpha-2 mobility, which possessed antigenic determinants common with siaa, eluted from sephadex g-200 at the same position as mammalian secretory components, and could be released from rat siga by mild reduction and alkylation. this protein, called rat secretory component, was also detected in saliva, tears and urine. rat free secretory component (fsc) ... | 1975 | 803998 |
| phylogeny of complement components in non-human primates. | the antigenic properties and functional activities of complement components were analyzed in primates to determine their relative evolutionary development. the sera of eight different sub-human primate species were examined by double diffusion in agar and compared to a pool of human serum with rabbit and goat antisera to human complement components clq, cls, c4, c2 c3, c5, c6, c8, c9, properdin, factor b (b), and c1 inhibitor. there are no apparent antigenic differences in complement proteins be ... | 1975 | 803999 |
| control of j chain biosynthesis in relation to heavy and light chain synthesis, polymerization and secretion. | mouse myeloma tumors and some variants derived from them were labeled in vitro with tritiated leucine and the radioactive j chain was assayed in cell lysates by precipitation with an antiserum specific for mouse j chain. the major findings were: 1) j chain can be found in an igg2b-secreting cells (mpc-11). these data, together with previous findings suggest that cells secreting all classes of igg synthesize j chain, even though there is no apparent requirement for j chain in assembly of the igg ... | 1975 | 804006 |
| measurement of kappa:lambda light chain ratios by haemagglutination techniques. | haemagglutination techniques are described for quantitating kappa:lambda ratios in human immunoglobulin. the techniques have been evaluated using monoclonal light chains from myeloma patients, but are applicable to a variety of clinical situations. | 1975 | 804013 |
| interstitial nephritis with anti-tubular-basement-membrane antibody. | to determine the pathogenesis of interstitial nephtitis, immunopathological studies were performed with kidney and serum from a six-year-old boy. the kidney revealed linear staining of tubular basement membranes with antiserums specific for human igg and c3; the membranes also showed fixation of heterologous complement. after incubation of the patient's serum on frozen sections from normal human kidneys, linear staining with igg was detected by indirect fluorescence. this staining was eliminated ... | 1975 | 804130 |
| immunochemical studies on the storage and axonal transport of neurophysins in the hypothalamo-neurohypophyseal system. | | 1975 | 804289 |
| antimyelin antibodies in neurologic diseases. immunofluorescent demonstration. | antimyelin binding activity was determined in patients with multiple sclerosis, amyotrophic lateral sclerosis, other neurologic diseases, and normals. there was an increase in the group mean titer of antimyelin antibodies in patients with multiple sclerosis (ms) in acute exacerbation, amyotrophic lateral sclerosis (als) and guillain-barré syndrome. a lesser degree of binding was found in normals of all ages. there was an increase in the incidence of antimyelin antibodies in the igm class in pati ... | 1975 | 804300 |
| [new knowledge on the biology of coccidia, toxoplasma, sarcosporidia and besnoitia]. | | 1975 | 804307 |
| identification of specific hemoglobins within individual erythrocytes. | the identification of specific antibodies for a variety of inherited hemoglobin variants presents the opportunity to develop sensitive immunochemical measurement techniques for such variants. in this study antibodies specific for human hemoglobins a1 and s were isolated from immune rabbit serum. these were labeled with either fluoroscein isothiocyanate or tetramethylrhodamine isothiocyanate. erythrocytes suspended in agar were incubated with these labeled antibodies and hemoglobins a1, s, and f ... | 1975 | 804334 |
| ehancement and hyperacute rejection of skin grafts in the mousefailure to separate cytotoxic and enhancing antibodies from alloantiserum with physicochemical methods. | b6af1 anti-b10.d2 ascites fluid was fractionated by molecular sieving and cation exchange chromatography in an attempt to separate cytoxic and enhancing antibodies. five fractions were obtained which showed no overlap on analysis with isoelectric focusing. despite this complete physicochemial separation all fractions contained detectable amounts of 7s igg1 and 7s igg2. furthermore, exhancement of b10.d2 skin grafts in b6af1 recipients could be induced with all the fractions. cytotoxic activity w ... | 1975 | 804518 |
| preparation and characterization of rabbit igg fractions. | rabbit igg has been purified and further fractionated by deae-cellulose column chromatography to yield three fractions, fr-i,-ii and -iii-1 that span a broad range in the heterogeneous molecular population of igg. immunodiffusion established that these fractions are free from contamination by iga, igm, transferrin, and hemopexin. the three fractions were assayed for neutral hexose and sialic acid; fr-iii-1 and fr-i yielded the highest and lowest values for these components, resepctively. the ord ... | 1975 | 804520 |
| healthy blood donors with selective absence of immunoglobulin a: prevention of anaphylactic transfusion reactions caused by antibodies to iga. | gel diffusion analysis of sera from 73,569 healthy volunteer blood donors revealed apparent lack of iga in 113 (1:650) samples, all with normal levels of igg and igm. of these, 30 sera had low levels of iga, while the remaining 83 had no iga detectable by a more sensitive hemagglutination inhibition assay. the frequency of absent iga (alga) was 1:886. high-titer anti-iga was detected in the sera of 13 alga donors; only two had any known history of parenteral injection of iga. passive transfusion ... | 1975 | 804531 |
| renal urate excretion in animal models. | several aspects of the comparative physiology and pharmacology of renal urate handling are considered; with special attention to bidirectional transport, the existence of intrarenal urate synthesis and degradation in certain species, and the nature and sites of the reabsorptive and secretory processes. interspecies variations in the sensitivity of the kidney to uricosuric agents and the degree of specificity of the secretory mechanism are discussed. a variety of techniques, especially as applied ... | 1975 | 804668 |
| immunologically-mediated renal disease in waldenström's macroglobulinemia. | a patient with walderström macroglobulinemia associated with nephrotic syndrome is described. serum cryoglobulin and rheumatoid factor were absent. intramembranous electron-dense deposits were demonstrated in kidney biopsy material by electron microscopy. deposits of immunoglobulin g (igg), m (igm) and the third component of complement (c3) were identified in kidney biopsy tissue by immunofluorescent staining methods. the serum immunoglobulins were characterized by chromatographic and immunochem ... | 1975 | 804814 |
| serologic classification of feline caliciviruses by plaque-reduction neutralization and immunodiffusion. | serologic classification of 14 isolated of feline caliciviruses was carried out, using plaque-reduction neutralization tests. sixty to 200 plaque-forming units of virus were employed against 20 antibody units of hyperimmune goat antiserums prepared to individual isolates. the results established that these viruses were related in various degrees, but no 2 were identical. arrangements in order of the greatest intergroup reactions yielded essentially 1 serotype with the exception of 1 virus showin ... | 1975 | 804838 |
| the interaction between sodium dodecylsulphate solubilized human ghosts and antisera to human serum lipoproteins: a non-immune precipitation effect of sodium dodecylsulphate. | | 1975 | 804921 |
| antibody production in milk serum after antigen instillation of the goat mammary gland. ix. sham infection studies with neisseria meningitudis l.c.d.c. 608b. | specific immune globulins have been prepared in goat milk in response to the intramammary gland instillation of neisseria meningitidis 608, serogroup b strain. isolation, purification, and characterization of the goat whey by gel filtration, electrophoresis, and analytical ultracentrifugation demonstrated that the active immune component resided in the iga class of globulins, specifically 9.2-s iga. the potential of the lactating mammary gland as a "biological factory" for the large-scale produc ... | 1975 | 804985 |
| inhibition of rat liver glucose-6-phosphate dehydrogenase synthesis by glucagon. | in fasting-refeeding experiments glucagon was found to inhibit the induction of rat liver glucose-6-p dehydrogenase. the kinetics of induction in the presence of glucagon indicated that the hormone decreased the rate of enzyme synthesis without altering the rate of enzyme degradation. immunochemical titration of the amount of glucose-6-p dehydrogenase protein present in control and glucagon treated rats suggests that glucagon does not produce an inhibited species of the enzyme. isolation of radi ... | 1975 | 805134 |
| binding parameters of the interaction between rat ige and rat mast cell receptors. | rat monoclonal ige was purified by a combination of gel filtration and isoelectric focusing. the binding parameters of the ige-target cell interaction were studied. the target cells used were normal rat peritoneal mast cells (rmc) and neoplastic rat basophilic leukocytes (rbl). binding assays were performed by adding different amounts of ige to a constant cell number. a scatchard analysis of the data indicated that both cell types have a similar affinity for rat ige; the equilibrium constant wa ... | 1975 | 805173 |
| bactericidal activity of meningococcal antisera. blocking by iga of lytic antibody in human convalescent sera. | humoral iga, separated and purified from human sera drawn 12, 27, and 33 days after infection with serogroups b, y, and c meningococci, respectively, has been shown to inhibit complement-mediated bacteriolysis by igg and igm purified from the same sera. inhibition was dependent on the ratio of lytic to blocking antibody, was strain specific, and was greater for igg than igm. igm was found to be more active than igg in a bactericidal test. activity of iga appeared unrelated to the presence of a p ... | 1975 | 805178 |
| the immune response of mice treated with anti-mu antibodies: the effect on antibody-forming cells, their precursors and helper cells assayed in vitro. | previous studies have shown that mice treated from birth with heterologous anti-mu antiserum are severely immunosuppressed with respect to numbers of splenic plaque-forming cells (pfc) to sheep red blood cells (srbc) in all immunoglobulin classes. in this study we have investigated, using in vitro techniques, the cellular site of the deficit created by anti-mu. the primary pfc response of spleen cells originating from anti-mu-treated mice was completely suppressed in vitro. the response was rest ... | 1975 | 805179 |
| immunological studies of the rat olfactory marker protein. | | 1975 | 805214 |
| preparation of precipitating antigens by coupling oligosaccharides to polylysine. | | 1975 | 805564 |
| metabolism of chlorobiphenyls in the goat and cow. | | 1975 | 805805 |
| listeria monocytogenes. isolation from sheep and goats in northern norway. serogrouping and some biochemical reactions. | listeriosis is a zoonosis of world-wide importance in many different animal species, especially cattle, sheep and goats. this report presents results of examinations of listeria monocytogenes strains from sheep and goats in northern norway. | 1975 | 806057 |
| role of carotene and vitamin a in animal feeding. | | 1975 | 806182 |
| [functions of the omasum in small domestic ruminants. ii. fermentation rate and dna content]. | | 1975 | 806195 |
| [functions of the omasum of small ruminants. iii. absorption of water]. | | 1975 | 806207 |
| the antigenicity in guinea pigs and monkeys of three mycobacterial polysaccharides purified by affinity chromatography with concanavalin a. | the antigenicity of 3 polysaccharides purified from culture filtrates of mycobacterim tuberculosis by affinity chromatography using a concanavalin a-agarose absorbent was studied. all 3 purified polysaccharides were found to be potent elicitors of delayed skin test reactions in sensitized guinea pigs and in a tuberculos monkey. this antigenicity could not be attributed to contaminating protein. small dermal reactions were also observed in control guinea pigs. all 3 polysaccharides reacted with p ... | 1975 | 806241 |
| antigen binding to lymphoid cells from unimmunized mice. ii. high frequency of antigen-binding cells for several protein antigens by a morphologically distinct ig-bearing population of t- and b-lymphocytes. | | 1975 | 806357 |
| antigen binding to lymphoid cells from unimmunized mice. iii. multiple antigen binding to single b- and t-cells. | | 1975 | 806358 |
| morphological changes of basophils in immunological reactions--effect of sodium cromoglycate. | the morphological changes in blood basophils, from patients with bronchial asthma, were studied in vitro during immunological reactions, with particular reference to interaction with anti-ige. anti-human ige results in an absolute reduction in basophil numbers in buffy coat preparations as well as basophil cytoplasmic degranulation. an increase in the number of pear-shaped (a-form) basophils is also observed. sodium cromoglycate impairs all these anti-ige induced basophil changes, providing furt ... | 1975 | 806398 |
| development of monospecific antibodies to rat hemopexin and albumin: evidence for immunological cross-reactivity with mouse proteins. | | 1975 | 806414 |
| dog immunoglobulins. i. immunochemical characterization of dog serum, parotid saliva, colostrum, milk and small bowel fluid. | the levels of immunoglobulins a, m and g were measured in dog serum, colostrum, milk, parotid saliva and small bowel fluid using the single radial immunodiffusion method. all the external secretions except early colostrum, by contrast with serum, were found to be rich in iga with small quantities of igm and igg. exocrine immunoglobulins were partially characterized by gel filtration. | 1975 | 806517 |
| initiation by methionine of mouse immunoglobulin light chain containing nh-2terminal pyroglutamic acid. | the mechanism of biosynthesis of nh2-terminal pyroglutamic acid has been studied in a mouse plasmacytoma (rpc-20) which produces an immunoglobulin light (lambda) chain containing nh2-terminal pyroglutamic acid. to this end, initation of lambda chain synthesis in plasmacytoma cell suspensions has been investigated. the analysis of radioactive lambda chain synthesis by these cells was accomplished with an antibody preparation specific for the precipitation of lambda chain protein from total plasma ... | 1975 | 806584 |
| divergent results in radial immunodiffusion with antisera differing in precipitation properties with respect to individual immunoglobulin subclass. i. the antigen factor. | two bovine igg subclasses were used as reference antigens to study subclass influence on radial immunodiffusion results with total bovine igg. in combination with some of eight anti-bovine igg sera tested the use of the same antiserum with one subclass furnished values which were about twice as high as values obtained with the other subclass. it is concluded that immunoglobulin determinations by radial immunodiffusion are influenced by the chosen reference antigen and by subclass ratios in the s ... | 1975 | 806635 |
| leptospirosis in ethiopia: a serological survey in domestic and wild animals. | seven hundred and fifty-eight serum samples from domestic and wild animals in ethiopia were tested for leptospiral antibodies by the microscopic agglutination test. the following percentages of seropositivity were obtained: horse (91.3 per cent), cow (70.7 per cent), pig (57.1 per cent), goat (47.3 per cent), sheep (43.4 per cent), camel (15.4 per cent), and dog (8.3 per cent). all 54 samples from birds and wild animals were seronegative. most of the positive sera exhibited reaction to more than ... | 1975 | 806698 |
| brucellosis in childhood. | brucellosis has always been an unusual disease in children and, concomitant with the control of the disease in domestic animals, reports have become sparse. the pediatrician, therefore, may not be aware of the protean clinical manifestations of childhood brucellosis. in 1973, nine cases occurred during a three-month period in el paso, texas. all cases were marked by spiking fevers and lethargy of four days to four weeks in duration. tender hepatomegaly or splenomegaly was striking in seven patie ... | 1975 | 806883 |
| biochemical and immunological studies on salivary blood group substances. | salivas with various phenotypes were analyzed for chemical components and blood group activity after ultracentrifugation. active blood group substances were isolated from saliva in the pellet form by sedimentation. both the supernatant and the pellet suspended in original volume were studied for content of fucose, total hexose, protein, and sialic acid and the presence of blood group activity. paper chromatography of these saliva pellets after acid hydrolysis demonstrated fucose, galactose, gluc ... | 1975 | 807038 |
| [functions of the omasum in small domestic ruminants. iv. resorption and secretion of electrolytes]. | | 1975 | 807059 |
| gestational changes in the endometrium of the goat. i. histomorphological changes. | | 1975 | 807061 |
| gestational changes in the endometrium of the goat. ii. histochemical characteristics. | | 1975 | 807062 |
| bone marrow histology in monoclonal macroglobulinemia. | rywlin, arkadi, m., civantos, francisco, ortega, rolando s., and dominguez, carlos j.: bone marrow histology in monoclonal macroglobulinemiamam j clin pathol 63. 769-778, 1975. histologic sections and smears of aspirated bone marrow particles in 26 cases of monoclonal macroglobulinemia were studied. the bone marrows did not show uniform histologic features. twenty-two patients had various degrees of lymphoid infiltration of the marrow, including nodules of malignant lymphoma, diffuse lymphocyti ... | 1975 | 807098 |
| studies on stimulation and properties of antiglobulin antibodies. i. influence of heatig at 56 degrees c and 60 degrees c on antigenicity. | the autoimmunogenic properties of whole rabbit serum heated at 56 degrees c and 60 degrees c were studied in rabbits. autologous rabbit serum heated 20 min at 60 degrees c was immunogenic. the state of induced immunity manifested itself by the appearance of antibodies to antigens of heterologous sera: human, goat and guinea pig. no antibodies for autologous sera were found, including autologous sera heated 20 min at 60 degrees c. the antibodies for heterologous sera directed against gamma-globul ... | 1975 | 807178 |
| clinical and immunologic features of selective iga deficiency. | selective absence of serum and secretory iga is probably the most common form of human immunodeficiency. high frequencies of recurrent sinusitis, otitis media, pneumonia, and atopy were noted among a group of 75 such patients, all but 4 of whom were caucasian. seven instances of familial absence of iga were detected among 106 relatives of 34 of the group; in 1 family 1 member from each of 3 successive generations was affected. two iga-deficient children were later found to have normal amounts of ... | 1975 | 807270 |
| hereditary c6 deficiency in man. | an 18-year-old black female (d.b.) in good general health was found to have no hemolytic activity in serum ch50 titrations. functional assays yielded normal values for all c components except c6. c6 was not detectable in plasma or serum by two different functional assays nor by antigenic analysis using monospecific anti-c6 antibody. hemolytic activity was restored by addition of functionally pure c6. by specific functional assay, both parents and 5 of 6 available sibs had approximately half-norm ... | 1975 | 807274 |
| [daily variations in plasma somatotropin in the lactating or dry goat]. | in lactating goats, manual milking, feeding and certain conditions depending on milking routine may increase plasma growth hormone during day time. growth hormone basal levels were higher in lactating goats than in dry goats. statistical difference between the means was highly significant. | 1975 | 807424 |
| membrane-bound immunoglobulins on human leukemic cells. evidence for humoral immune responses of patients to leukemia-associated antigens. | immunoglobulins were detected on the membranes of human leukemic cells by a microcytotoxicity technique. a significant percentage of lymphocytes from normal donors failed to react with goat antisera to human heavy chain determinants or to lambda-light chains. lymphocytes from some normal donors, however, did react with antisera to k-light chains. a high percentage (50-90) of cells from some leukemia patients were killed by antisera to light chains and by one or more antisera to heavy chain deter ... | 1975 | 807598 |
| mycoplasmas isolated from the respiratory tract of horses. | ten mycoplasmas were isolated from 130 nasopharyngeal swabs from thoroughbred horses with acute respiratory disease and three from 198 apparently normal horses. two mycoplasmas were isolated from 21 tracheal swabs taken at necropsy. these mycoplasmas, together with six isolated from the equine respiratory tract by other workers, were subjected to biochemical and serological tests. other properties examined in certain representative strains were appearance under the electron microscope, ability t ... | 1975 | 807616 |
| naturally occurring low molecular weight igm in patients with rheumatoid arthritis, systemic lupus erythematosus and macroglobulinemia. i. purification and immunologic studies. | a method for purification of low molecular weight igm (lmw igm) which utilized salt precipitation, gel filtration, and insoluble immunoadsorbents is described. the yield from pathologic sera containing lmw igm in the range of 15 to 36 mg% was approximately 20%. antisera prepared against lmw igm, igm, and reduced-alkylated igm recognized common determinants. purified lmw igm from seropositive rheumatoid subjects did not have rheumatoid factor activity in agglutination tests but demonstrated bindi ... | 1975 | 807624 |
| isolation of pure igg subclasses from mouse alloantiserum and their activity in enhancement and hyperacute rejection of skin allografts. | b6af1 anti-b10.d2 ascites fluid was fractionated by molecular sieving. from the 7s fraction, igg subclasses were isolated by affinity chromatography with specific antisera against mouse immunoglobulin subclasses coupled to cnrr-activated sepharose 4b. thus, 7s igg1, 7s igg2, 7s igg2a, and 7s igg2b were obtained which were completely pure by criteria of immunodiffusion and immunoelectrophoresis. in the in vitro tests the 7s igg1 completely lacked cytotoxic activity. all the other subclasses were ... | 1975 | 807636 |
| differential susceptibility of rabbit siga subclasses to trypsin cleavage: characterization of the fragments obtained from the g subclass. | rabbit secretory iga (siga) was digested with trypsin at 37 degrees c for 30 min and four fractions were isolated by gel filtration. these fragments were characterized by ultracentrifugation, and by their antigenic properties as undigested siga, fab and fe (two fc fragments differing in their content of secretory component were obtained.) the iga-f subclass molecules were resistant to cleavage and were found in the undigested siga fraction; the iga-g subclass molecules were cleaved into fe and f ... | 1975 | 807638 |
| b lymphocyte differentiation induced by lipopolysaccharide. ii. response of fetal lymphocytes. | cultures of mouse fetal liver and spleen, stimulated by bacterial lipopolysaccharide, gave rise to plasma cells staining for igm, igg2, igg1, and iga. cells containing igm and igg2 were found in cultures from 17-day fetuses, coincident with the appearance of b lymphocytes bearing cell-surface igm. igg1- and iga-containing cells were induced in cultures from 19-day fetuses and 1-day-old mice. the capacity to give rise to immunoglobulin-secreting cells of all classes preceded the development of a ... | 1975 | 807645 |
| comparative study of proteins extracted form metal-induced allergic and foreign body granulomas in man. | in order to characterize proteins unique to organized epitheloid cells, proteins havebeen sequentially extracted form both foreign body and allergic granulomas in man at varoius times after intradermal injection of beryllium oxide suspension. treitium-labeled l-tyrosine was injected intralesionally 2 weeks before excision of granulomas. prolongedextraction with 8 m urea yeilded increases amounts of radioactivie protein form older (8-to 26-week) allergic granulomas but not from 4-to6-week-old or ... | 1975 | 807777 |
| [parathormone determination with synthetic human parathormone 1-34 (pth) (author's transl)]. | using synthetic human parathormone 1-34, a radioimmunological method for measuring this hormone fragment was developed which, as the amino-terminal pth assay, permits the measurement of the concentration gradient in the neighborhood of oth-secreting tumors. the use of preoperative location diagnosis in primary hyperparathyroidism is demonstrated with some typical cases, especially in revision operations. | 1975 | 807827 |
| immunochemical and functional similarities and differences among iron-sulfur proteins involved in mammalian steroidogenesis. | an antibody prepared against bovine adrenodoxin has been employed to study possible similarities or differences among the iron-sulfur protein components of electron transport sequences associated with cytochrome p-450 function in the mitochondria of mammalian steroidogenic tissues. although they are not identical, the "adrenodoxins" in mitochondria from the adrenals of a number of species, including man, are both immunochemically and functionally similar. the ability of the antibody to inhibit a ... | 1975 | 808111 |
| studies on schistosoma bovis in ethiopia. | schistosoma bovis occurs in at least seven of the 14 rovinces of ethiopia. results of faecal and snail surveys in three foci are reported. adwa. one collection showed that nine out of 26 bulinids were infected with s. bovis. the snail host was a tetraploid form of bulinus (n = 36). the examination of 200 specimens of cattle faces revealed no s. bovis eggs, which was attributed to poor technique or light infection. gewani. the snail host was bulinus abyssinicus, which was also infected with s. ha ... | 1975 | 808181 |
| [effects of milking on plasma levels of somatotropic hormone in sheep and goats]. | growth hormone is released after hand-milking in the ewe and in the goat. species, individual and diurnal variations are observed as well as during the lactation period. | 1975 | 808280 |
| [concentrating ability of the gallbladder: a comparative study in some vertebrate species (author's transl)]. | the bile salts, chloride, sodium, potassium and nitrogen content of the gallbladder bile was studied in goat, sheep, cattle, pig, rabbit, dog, monkey (erythrocebus patas), chicken, lizard (lacerta ocellata), frog (rana esculenta), trout (salmo gardnieri) and barbel (barbus barbus). a significant correlation was found between bile salts and sodium (positive), chloride and sodium (negative) and chloride and bile salts (negative). the gallbladder concentrating ability was very high in monkey, lizar ... | 1975 | 808839 |
| methods for studying oviductal physiology. | in studying oviductal physiology, it is important to sort out the complex interrelationships between muscle, cilia, nerves and secretory processes as they each of themselves, or in concert, effect gamete transport. in this review, a variety of physiological techniques and bioengineering approaches which have been used to monitor contractile and ciliary activity, are described and critically evaluated. | 1975 | 809314 |
| egg transport in the fallopian tube. | the transport of eggs from the site of ovulation to the site of implantation is a fundamental step of the reproductive process in the female. the fallopian tube effects the major part of this function and times the passage of eggs into the endometrial environment. as a result of different combinations of speed of progression and retention periods through the various regions of the oviduct, the pattern of transport differs from one species to another. the role of muscular contraction, ciliary mov ... | 1975 | 809316 |
| bacterial hemagglutination by neisseria gonorrhoeae. | direct bacterial hemagglutination was investigated with 20 clinical isolates of neisseria gonorrhoeae. the hemagglutination tests were performed by both a macrotechnique with glass slides and a microtechnique with autotrays. only organisms from form type 1 or 2 colonies caused hemagglutination. there was no statistical difference at a 10% or higher level in hemagglutination powers of type 1 and type 2 organisms, of male urethral and female cervical isolates, and of the eight major human blood ty ... | 1975 | 809353 |
| axonal projections and connections of the principal sensory trigeminal nucleus in the monkey. | to date, anatomical studies of ascending principal sensory trigeminal nuclear (prv) axons in the monkey have been restricted to few incomplete investigations utilizing the marchi method. in the present study total or partial unilateral stereotaxic lesions of prv were made in cebus and rhesus monkeys and analyzed with the aid of a variety of nauta silver impregnation techniques applied to frozen sections. analysis of the fiber degeneration emanating from prv lesions indicates that prv fibers form ... | 1975 | 809492 |
| characterization of a chemotactic factor (leucoegresin) for polymorphonuclear leucocytes from thermal skin site. | a chemotactic factor for pmn leucocytes was extracted in the pseudoglobulin fraction of burned skin lesions in rabbits and then highly purified by chromatography using sephadex g-50, deae-sephadex a-50 and cm-sephex c-50 in this order. chemotactic activity was estimated by a modification of boyden's method during the process of purification. this factor was a protein free of nucleic acid and its molecular weight was approximately 140000 when measured by gel filtration. its activity was relativel ... | 1975 | 809922 |
| immunohistological demonstration of insulin and glucagon in islet tissue of reptiles, amphibians and teleosts using epoxy-embedded material and antiporcine hormone sera. | islet tissue from 2 reptilian, 5 amphibian and 7 teleost species was fixed in glutaraldehyde, embedded in epoxy-resin, stored for up to 3 years, cut in ultrathin sections and stained with the indirect immunofluorescent technique for insulin and glucagon following removal of the plastic. the antisera were directed against porcine insulin and glucagon, or fitc labeled and directed against the globulin fraction of the producer species. positive results were obtained in species from all vertebrate c ... | 1975 | 809978 |