| partial characterization of basic proteins of chicken, turtle and frog central nervous system myelin. | | 1975 | 45933 |
| letter: national dried milk. | | 1975 | 46066 |
| failure to confirm separation of x- and y-bearing human sperm using bsa gradients. | | 1975 | 46109 |
| histamine release during antigen inhalation in experimental asthma in dogs. | histamine release after antigen inhalation was studied in 12 dogs sensitive to nematode antigens. in 19 experiments, histamine was detected in arterial plasma after antigen inhalation; its concentration was correlated with changes in airflow resistance of the respiratory system (rrs) (per cent change above control equals 132.3 plus 250 log histamine concentration). histamine was released from the lung and was not attributable to the concomitant hypoxia. in 6 dogs given aerosols of dilute antigen ... | 1975 | 46138 |
| characterization of factor viii antibodies. | | 1975 | 46139 |
| search for c-type particles in human neoplasia. | the salient biologic and morphologic characteristics of rna tumor (oncornavirus) virus are reviewed. the ultrastructure of replicating oncornaviruses is illustrated in detail. c-type particles wide spread in at least three orders of animals were sighted in human sarcomas and leukemias. one case, an infantile fibrosarcoma, is presented from our cases surveyed for the presence of c-type particles. tissue cultures derived from this tumor contained viral particles and had an elevated reverse transcr ... | 1975 | 46142 |
| serum-stimulated lipases (lipoprotein lipases). immunological crossreaction between the bovine and the human enzymes. | a rabbit antiserum prepared against the serum-stimulated lipase (lipoprotein lipase) from bovine milk crossreacted with serum-stimulated lipases from human milk and from human postheparin plasma, but not with bile salt-stimulated lipase from human milk or with salt-resistant lipase from human postheparin plasma. thus, the serum-stimulated lipase in bovine milk has immunological determinants in common with the serum-stimulated lipases in human milk and in human postheparin plasma. the time-course ... | 1975 | 46150 |
| a gene locus affecting tolerance to bgg in mice. | | 1975 | 46187 |
| thermal denaturation of dna in situ as studied by acridine orange staining and automated cytofluorometry. | | 1975 | 46199 |
| differential chromosomal fluorescence with 33258 hoechst. | | 1975 | 46201 |
| bovine veneral vibriosis: antigenic variation of the bacterium during infection. | host parasite relationships in the female genital tract were studied in bovine venereal vibriosis by investigating agglutinin production and alterations in superficial antigens of the bacterium during the course of infection in two heifers. cervicovaginal mucus (cvm) steamed cell agglutinins were shown to appear earlier and remain at consistently higher levels than whole-cell agglutinins. whole-cell agglutinin titers fluctuated much more than steamed cell titers, suggesting possible changes in w ... | 1975 | 46214 |
| allergic encephalomyelitis. isolation of an encephalitogenic peptide active in the monkey. | a 17-residue peptide (peptide y) was isolated from the cooh-terminal end of the basic protein of bovine myelin by peptic digestion. this peptide induced experimental allergic encephalomyelitis in the rhesus monkey. treatment of peptide y with cyanogen bromide released three amino acids from the cooh-terminal end and resulted in a tetradecapeptide (peptide m) which was also encephalitogenic in the rhesus monkey. the sequence of peptide m is: phe-lys-leu-gly-gly-arg-asp-ser-arg-ser-gly-ser-pro-met ... | 1975 | 46230 |
| carrier-determined tolerance in vitro. | a primary immune response to normal bdf1 spleen cells was obtained in vitro to the t-independent antigen tnp-t4 coliphage. this anti-tnp response was suppressed by exposing the spleen cells for 6 hr to tnp bound either to isologous or heterologous gamma-globulin. the suppression was hapten specific. in contrast, tnp-albumin conjugates did not induce tolerance in vitro. | 1975 | 46237 |
| spontaneous and methylcholanthrene-enhanced thyroiditis in buf rats. ii. induction of experimental autoimmune thyroiditis without completed freund's adjuvant. | the immunization of buffalo strain (buf) rats with rat thyroid extract and bordetella pertussis vaccine without complete freund's adjuvant resulted in experimental autoimmune thyroiditis (eat) in 90% of the rats by the 10th day after immunization. the treatment did not elicit thyroiditis in lewis strain (lew) rats. this susceptibility to the induction of eat without the use of complete freund's adjuvant was inherited by f1 hybrids of buf and lew. neither histamine sensitizing factor from b. pert ... | 1975 | 46238 |
| antigenic relationship between the fourth component of human and guinea pig complement. | antigenic similarity between cell-bound human and guinea pig c4 was detected with antisera specific for each of these molecules. no cross-reaction could be detected between c4 of normal guinea pig or human sera or after treatment of the sera with antigen-antibody c1 complexes. the cross-reactivity between cell-bound human and guinea pig c4 may reflect conformational changes in the c4 molecule as a result of binding of the cell surface. | 1975 | 46239 |
| specific isolation and characterization of antibody directed to binding site antigenic determinants. | the preparation and specificity of antibodies specific for the ligand-binding site of hopc 8, a phosphorylcholine (pc)-binding mouse myeloma protein, are described. antiserum to hopc 8, prepared in rabbits, was adsorbed with an hopc 8-sepharose immunoadsorbent and anti-binding site antibodies were eluted with pc. these antibodies reacted with hopc 8 but not other myeloma proteins, including those with pc-binding specificity different from hopc 8; the specificity of this anti-hopc 8 antibody for ... | 1975 | 46246 |
| immunologic properties of bacterial lipopolysaccharide (lps): correlation between the mitogenic, adjuvant, and immunogenic activities. | bacterial lipopolysaccharide (lps) was demonstrated to have the capacity in mice to enhance the response to soluble bovine serum albumin (bsa) and to interfere with the induction of tolerance to human gamma-globulin (hgg). these adjuvant activities were shown to occur under conditions in which lps could also function as a b cell mitogen. this positive correlation was established by utilizing two experimental situations in which lps was non-mitogenic for spleen cells. thus, on the one hand, it wa ... | 1975 | 46249 |
| the antigenicity of myelin encephalitogenic protein: production of antibodies to encephalitogenic protein with deoxyribonucleic acid--encephalitogenic protein complexes. | rabbits immunized with monkey encephalitogenic protein (ep) complexed to deoxyribonucleic acid (dna) formed complement-fixing antibodies that reacted well with ep from monkey, bovine, and human brain and the large ep of rat brain. reactivity with the small ep of rat brain was much less. the antibodies, analyzed by the quantitative micro-complement fixation test, were of the 7s class, were produced in high titer, and failed to react with histone, lysozyme, or acidic liver extracts. immunohistoche ... | 1975 | 46251 |
| complement-mediated release of histamine from human leukocytes. | activation of either the alternative or classical pathway of complement generated a factor which induced release of histamine from both non-allergic and allergic human basophils. this factor probably is derived from the complement system since 1) its formation was associated with loss of c3 activity in human serum, 2) chemotactic factor, probably also a complement product, was generated simultaneously, 3) heat inactivation blocked its formation, 4) anti-c3 and anti-c5 blocked formation of the fa ... | 1975 | 46261 |
| the hapten-carrier effect in teleost fish. | the sea robin (prionotus evolans), a representative teleost fish, was found to undergo the hapten-carrier effect, suggestive of t-b cell cooperativity, in vivo with dinitrophenyl as the hapten and bovine gamma-globulin or bovine serum albumin as carriers. in contrast to mammalian systems, the secondary anti-hapten response in the sea robin was found to involve exclusively high molecular weight antibody. | 1975 | 46263 |
| localization of prostaglandin on the plasmalemma of rabbit sperm. | prostaglandin and prostaglandin receptors were localized on unwashed ejaculate rabbit sperm utilizing ferritin conjugated antibody to prostaglandin. reaction was obtained over the acrosomal portion of the plasma membrane, with a greater aggregation over the apex of the head. the remainder of the head plasmalemma and flagellum showed no deposits of of reaction product. the reaction did not occur if the sperm were washed prior to incubation with the labelled antisera. however enhanced binding of f ... | 1975 | 46269 |
| an attempt to identify the intestinal receptor for the k88 adhesin by means of a haemagglutination inhibition test using glycoproteins and fractions from sow colostrum. | the k88 antigen of escherichia coli specifically adheres to the piglet intestinal cell; a solution of this antigen agglutinates guinea-pig red cells at 4 degrees c. the latter reaction was used as a model of the former, using inhibition of haemagglutination as an index of specific combination with the k88 adhesin. inhibition was found with mucous glycoproteins and chemical modification of their heterosaccharide residues by mild acid hydrolysis, periodate oxidation or the smith degradation proced ... | 1975 | 46271 |
| experimental allergic optic neuritis (eaon) in the rabbit. a new model to study primary demyelinating diseases. | in new zealand albino rabbits the axons of the nerve fibre layer are myelinated over a long distance within the retina. primary demyelination of these fibres was induced by sensitization of the animals to bovine cerebral white matter or myelin basic protein in complete freund's adjuvant and challenge thereafter by intravitreous injection of basic protein (bp) or purified tuberculin (ppd). production of segmental demyelination as a result of sensitization to brain-specific antigen (bp) and indiff ... | 1975 | 46272 |
| editorial: rotaviruses of man and animals. | | 1975 | 46393 |
| editorial: thymus hormones. | | 1975 | 46451 |
| uracil-specific anti-r.n.a. antibodies in scleroderma. | antibodies to single-stranded r.n.a. were found by counter immunoelectrophoresis in all of 40 sera from patients with scleroderma. these antibodies were specific to the uracil bases of r.n.a. antibodies to r.n.a. were also found in 20 of 40 sera from patients with systemic lupus erythematosus (s.l.e.), but in none of forty controls. antibodies to r.n.a. found in s.l.e. sera could be differentiated immunochemically from those found in scleroderma in that they were more heterogeneous and could rea ... | 1975 | 46513 |
| letter: lateral asymmetry and gonadal differentiation. | | 1975 | 46555 |
| ir-gene control of immunogenicity of insulin and a-chain loop as a carrier determinant. | | 1975 | 46593 |
| [procollagen (author's transl)]. | | 1975 | 46613 |
| a staining method using acridine orange and auramine o for fungi and mycobacteria in bovine tissue. | a rapid staining method using auramine o and acridine orange (aoao) is described for staining mycobacteria and fungi in paraffin sections of bovine tissues. one hundred seventy-seven tissue sections from specimens divided into two general groups on the basis of previous histopathology results were examined with a fluorscent microscope. group i, a total of 77 sections, were from 47 mycobacterial and 30 fungal granulomas. mycobacteria were found by the aoao procedure in 44 of the 47 tissues previo ... | 1975 | 46637 |
| induction of b lymphocyte tolerance by an oligovalent antigen. i. influence of the read-out system. | | 1975 | 46784 |
| migratory patterns of b lymphocytes. i. fate of cells from central and peripheral lymphoid organs in the rabbit and its selective alteration by anti-immunoglobulin. | | 1975 | 46789 |
| the heart as a target organ in systemic allergic reactions: comparison of cardiac analphylaxis in vivo and in vitro. | the purpose of this investigation was to define and quantitatively evaluate cardiac anaphylaxis in vivo. guinea pigs, passively sensitized with graded amounts of rabbit antipenicilloyl antibody, were anesthetized, ventilated, and challenged intravenously with a constant amount of antigen (anaphylaxis in vivo). in other experiments, guinea pig hearts were excised, perfused in a langendorff apparatus, and challenged (analphylaxis in vitro). during in vivo anaphylaxis, sinus rate increased 10-30 be ... | 1975 | 46793 |
| ontogeny of b-lymphocyte function with respect to the heterogeneity of antibody affinity-1,2. | neonatal liver or adult spleen was used as a source of b-lymphocytes in reconstituting lethally irradiated, syngeneic mice. recipients were all given excess adult, syngeneic thymus cells and were immunized with dinitrophenylated bovine gamma globulin. the distribution of avidities of plaque-forming cells produced by immunized recipients of neonatal liver was highly restricted in comparison with animals reconstituted with adult spleen indicating a restriction of b-lymphocyte heterogeneity in the ... | 1975 | 46833 |
| stimulation of phagocytic activity in the reticuloendothelial systems of mice by lipid a complexed with homologous or heterologous proteins. | heterologous proteins are more suitable carriers than homologous proteins for the expression of endotoxic activity of lipid a prepared from the lipopolysaccharides of salmonella typhimurium sl1102 (re form) to stimulate the reticuloendothelial system of mice. | 1975 | 46844 |
| half-lives of two types of rat homocytotropic antibodies in circulation and in the skin. | the half-lives of two classes of rat immunoglobulins with homocytotropic properties, i.e., ige and igg2a, in the circulating blood and in the skin were studied. the catabolism of both normal (reaginic) and pathological (myeloma) ige proteins in circulation was found to be extremely rapid with a half-life of 12 h. in contrast, the half-life of ige antibody in the homologous skin was calculated to be 7.4 days. on the other hand, the half-life of igg2a in circulation was about 5 days regardless of ... | 1975 | 46847 |
| comparsion of the properties of two antigen-induced guinea pig lymphokines. | the properties of macrophage migration inhibition factor (mif) and mitogenic factor (mf) were compared using culture supernatants of antigen-stimulated lymph node cells from inbred guinea pigs. gel filtration on sephadex g-100 indicated molecular weights of about 60,000 and 25,000 for mif and mf, respectively. the lymphokines also differed with respect to heat sensitivity, mif being largely inactivated by 60 degrees c for 20 min, whereas mf was unaffected by this treatment. the time course of pr ... | 1975 | 46849 |
| protein migration into nuclei. i. frog oocyte nuclei in vivo accumulate microinjected histones, allow entry to small proteins, and exclude large proteins. | a technique is presented which enables one to measure the extent to which a protein enters and accumulates in the nucleus of the frog oocyte. in this method, the protein, labeled with 125-i, is microinjected into the oocyte. after incubation, the oocyte is manually enucleated and the radioactivity in the nucleus and cytoplasm is determined. using this technique, proteins lighter than 20,000 daltons were found to enter the nucleus and completely equilibrate between the nucleus and cytoplasm withi ... | 1975 | 46868 |
| strain differences in ease of tolerance induction to bovine gamma-globulin: dependence on macrophage function. | dba/2 mice are highly susceptible while balb/c mice are resistant to the induction of tolerance with ultracentrifuged bgg. as measured by the clearance of a test dose of 125-i-bgg, 2 mg ultracentrifuged bgg is sufficient to induce complete tolerance in dba/2 mice, while balb/c mice exhibit a clearance rate similar to immune controls. balb/c mice pretreated with carrageenan (a macrophage toxic agent) lose their resistance to tolerance induction with 2 mg ultracentrifuged bgg, while treatment with ... | 1975 | 46883 |
| specificity of basophils and lymphocytes in cutaneous basophil hypersensitivity. | using a rosetting technique, it was found that the vast majority of basophils circulating in the blood or accumulating in the skin reactions of guinea pigs primed for cutaneous basophil hypersensitivity (cbh) lacked demonstrable specificity for sensitizing antigen, whether sheep erythrocytes, a soluble protein, or tumor cells. by contrast, one-third of cells teased from late skin reactions formed specific rosettes as did nearly 80% of circulating basophils in animals receiving repeated doses of ... | 1975 | 46884 |
| studies on the control of antibody synthesis. ix. effect of boosting on antibody affinity. | the effect of boosting on antibody affinity was studied in a haptenic system. generally, boosting results in the prompt synthesis of high affinity anti-hapten antibody. however, repeated boosting frequently leads to a decrease in the amount and affinity of the serum antibody. repeated boosting with hapten on a carrier different from that used for priming selectively stimulates synthesis of the highest affinity anti-hapten antibody and does not result in a decrease in affinity. priming with solub ... | 1975 | 46896 |
| radioelectrocomplexing: a counterelectrophoretic method for hapten--antibody interaction. | we have described an application of a radioimmunoassay (ria)method, known as radioelectrocomplexing (rec), which involves the anodal migration of antigen and the cathodal migration of antibody in agar electrophoresis. the agar is divided into zones of free antigen (dnp125i-hsa) and antigen bound with anti-dnp. complete assays of anti-dnp can be performed in 2-4 hr since both immune complex formation and separation of free from bound antigen can be accomplished by electrophoresis in 60-90 min. es ... | 1975 | 46903 |
| fetuin, an inhibitor of lymphocyte transformation. the interaction of fetuin with phytomitogens and a possible role for fetuin in fetal development. | fetuin, the bovine alpha-fetoprotein, contains glycopeptide sequences similar to those found on red cells. as a result, it is capable of strong physical interaction with the phytohemagglutinin isomitogens (h-phap) which possess two or more r (red cell binding) subunits as part of their tetrameric structures. fetuin shows little or no interaction with l-phap, a phytohemagglutinin made up of four l subunits which also lack red cell affinity. despite these differences fetuin is able to inhibit both ... | 1975 | 46911 |
| evidence for specific suppression in the maintenance of immunologic tolerance. | specific suppressor cells have been demonstrated in mice tolerant to the thymus-dependent antigen hgg. transfer of normal thymocytes, normal spleen cells, or immune spleen cells into these tolerant mice did not restore immunocompetence to hgg. furthermore, the transfer of tolerant spleen cells into normal recipients abrogated the response of these recipients to subsequent challenge with immunogenic hgg. spleen cells removed from mice 5, 8, or 11 wk after the induction of tolerance specifically s ... | 1975 | 46917 |
| neuritogenic and encephalitogenic properties of the peripheral nerve basic proteins. | two basic proteins, p1 of molecular weight 14,200 and p2 of molecular weight 12,300, purified from bovine peripheral nerve, were assayed for biological activity. the p1 protein is an exclusively neuritogenic agent, capable of producing clinical signs of experimental allergic neuritis (ean) and histological abnormalities in the peripheral nervous system (pns) of guinea pigs and rabbits, without any changes in their central nervous system (cns). p2 protein, like the cns basic encephalitogenic prot ... | 1975 | 46920 |
| antibodies to fentanyl. | immunization of rabbits with carboxyfentanyl-bovine gamma-globulin conjugate induced antibodies of high titers capable of binding with fentanyl. the antibodies exhibited high average association constants (approximately 1 times 10-7 liter/mol) and were highly specific to fentanyl since no cross-reactivity was observed for a variety of test compounds including other opiates and analgesics. serological cross-reactivity was observed only for chemicals representing various portions of the fentanyl ... | 1975 | 46922 |
| letter: stimulation of prostaglandin biosynthesis by capsaicin, ethanol, and tyramine. | | 1975 | 47139 |
| chemistry of heparin. a short review on recent chemical trends. | | 1975 | 47192 |
| pharmocokinetics of heparin. distribution and elimination. | | 1975 | 47194 |
| antimicrobial drugs in food-producing animals. control mechanisms of governmental agencies. | | 1975 | 47207 |
| treatment of clinical mastitis: two intramammary formulations compared. | two hundred cases of mild clinical mastitis were treated on two farms using two intramammary preparations in quick release bases. one preparation contained penicillin and streptomycin, the other contained lincomycin, neomycin and prednisolone. fifty-eight per cent of cases were clinically and bacteriologically cured using the first preparation; and 61 per cent of cases, with a similar range of organisms, using the second. the main infections were streptococcus uberis and coliform; all but three ... | 1975 | 47208 |
| growth-promoting alpha-globulin and ageing. | | 1975 | 47217 |
| on basic proteins in bovine peripheral nerve myelin. | 1. myeline proteins in bovine peripheral nerve migrated as two main band-(bf and br protein) and one faint middle band (bm protein) on sodium dodecyls sulfate-polyacrylamide gel electrophoresis. the relative mobility of these two main bands differed from those of myelin proteins in the central nervous system. 2. the acid extract of the myelin fraction from bovine peripheral nerve was separated into one main peak and two minor peaks on a sephadex g-75 column. the major component of the second min ... | 1975 | 47245 |
| tissue specificity of nuclear acidic proteins isolated from bovine brain and adrenal medulla. | nuclear acidic proteins from bovine brain and adrenal medulla demonstrated dissimilar electophoretic and chemical profiles. the amino acid analysis of the acidic nuclear protein fraction isolated from these tissues revealed some variation in the ratio of acidic to basic amino acids. the estimation of free carboxylic acid groups confirmed the more acidic nature of the brain proteins. in comparing the acrylamide gels either visually or optically, several electrophoretically specific bands were app ... | 1975 | 47259 |
| the interaction of actinomycin c3 and actinomine with dna. a small-angle x-ray scattering study. | small-angle x-ray scattering was applied to solutions of calf thymus dna and calf thymus dna complexed with various amounts of actinomycin c3 or actinomine in phosphate-saline buffer at ph 6.9 and i equals 0.2. from the measurements of dna in the absence of dye, two cross-section radii of gyration of r-c equals 0.875 plus or minus 0.015 nm and r-c2 equals 0.81 plus or minus 0.02 nm, and a mass per unit length of m/l equals 1906 plus or minus 43 daltons/nm resulted. the investigation of dna compl ... | 1975 | 47290 |
| carrier and hapten functions in immune deviation. i. contrary effects of hapten and carrier on cellular and helper functions of t cells. | carrier and hapten functions have been studied in the immune deviation phenomenon. delayed hypersensitivity to the carrier and anaphylaxis and arthus hypersensitivities to the hapten and to the carrier were studied in guinea-pigs injected intravenously with large doses of carrier, homologous and heterologous hapten-carrier conjugates and subsequently immunized with the hapten-carrier conjugate in freund's complete adjuvant. pretreatment with dnp-bsa or with hgg were found to modify, in opposite ... | 1975 | 47307 |
| ultrastructure and adhesion properties of ruminococcus albus. | morphological studies have shown that cells of the anaerobic rumen bacterium ruminococcus albus have electron-translucent granules of reserve carbohydrate in their cytoplasm, and that they have a polysaccharide "coat" layer external to their gram-negative cell wall. this coat layer, which stains specifically with ruthenium red, forms a compact mat of fibers adjacent to the cell, and fibrous elements also project as much as 0.6 mum from the cells. these radial fibers are clearly visualized by fre ... | 1975 | 47323 |
| variation with age and disease of an amyloid a protein-related serum component. | using the radioactively-labeled alkaline-degraded acid-soluble fraction of amyloid ([ 125i ]daa), we developed a radioimmunoassay for the previously described amyloid-related component of the human serum (saa). screening the sera of 228 normal individuals and of 297 patients with a variety of illnesses, we found that saa is a component of all human sera, including cord blood (mean 94 plus or minus 57 ng/ml). the concentration of this component increases significantly with the aging process, reac ... | 1975 | 47333 |
| association of tissue factor activity with the surface of cultured cells. | tissue factor occurs in a dormant state on the surface of cultured normal human fibroblasts and wish 1 amnion cells. the activity of undisturbed monolayers or cells lifted with brief trypsin treatment (0.125 per cent trypsin for 1 min) increases up to 60-fold upon prolonged digestion with dilute trypsin (0.0025 per cent trypsin for 30 min); activity appears subsequent to cell detachment. up to 70 per cent of the total cellular tissue factor becomes active under these conditions and is released f ... | 1975 | 47334 |
| organochlorine content of milks, dairy products and animal feed ingredients: ireland 1971-1972. | milks (bovine and human) and dairy products (butter, cheese, skim and whey powders, calf-replacer, casein, butter-oil and dietetic food) were collected during 1971/2 throughout ireland together with a more limited samples of the 10 major animal feed ingredients, and analysed for organochlorine insecticide residues using electron-capture gas chromatography. the different materials contained low or negligible levels of chlorinated insecticides. apart from some of the animal feed ingredients the dd ... | 1975 | 47345 |
| the location of regions in guinea pig and bovine myelin basic proteins which induce experimental allergic encephalomyelitis in lewis rats. | myelin basic proteins and peptides derived from them by limited cleavage with pepsin were tested for their ability to induce experimental allergic encephalomyelitis (eae) in lewis rats. the encephalitogenicity of the weakly active bovine protein was found to be associated with both halves of the molecule, peptides (1-88) and (89-169). of the four smaller derivates of peptide (1-88), peptides (1-36), (43-88), (1-42), and (37-88), only the last two were active. this demonstrated that the overlap r ... | 1975 | 47354 |
| reaginic antibody formation in the mouse. v. adoptive antihapten ige antibody response of dinitrophenyl-keyhole limpet hemocyanin-primed spleen cells cultured with dinitrophenyl heterologous carrier conjugates. | dba/1 mice were primed with dinitrophenyl-keyhole lympet hemocyanin (dnp-klh) included in aluminum hydroxide gel, and the adoptive anti-dnp ige antibody response of their spleen cells was studied by transfer into irradiated syngeneic mice. as expected, depletion of t cells by anti-theta antiserum and complement abolished the response of the spleen cells to homologous antigen. if the same t-depleted spleen cells were cultured with dnp-klh for 24 hr and then transferred into irradiated mice, anti- ... | 1975 | 47357 |
| neural antigens and induction of myelination inhibition factor. | rabbits were sensitized or immunized with a variety of central nervous system antigens, including bovine spinal cord, bovine, monkey, human, guinea pig, rabbit and rat s myelin basic proteins, and a polypeptide derived from guinea pig basic protein. the animals were observed for development of experimental allergic encephalomyelitis, and their sera were collected at varying intervals after inoculation and evaluated for presence of precipitating anti-basic protein antibody and for their ability t ... | 1975 | 47359 |
| in vitro response of mouse spleen cells to the solid phase immunogen dnp-o-bio-gel. | the in virto immunogenicity of the solid-phase hapten, dinitrophenyl-ornithine-bio-gel (dnp-o-bio-gel), was investigated in cultures of mouse spleen cells. appropriate combinations of cells and immobilized hapten were determined. large numbers of direct anti-hapten plaque-forming cells (pfc) were generated when 1 times 10-7 c57bl/6 or c57bl/10 spleen cells were cultured with 4 times 10-3 dnp-o-bio-gel beads. specificity studies of the responses of cultured spleen cells to dnp-o-bio-gel yielded t ... | 1975 | 47361 |
| modulation of cyclic amp in purified rat mast cells. i. responses to pharmacologic, metabolic, and physical stimuli. | the cyclic adenosine 3', 5'-monophosphate (camp) content of isolated unstimulated mast cells and the changes induced by a variety of pharmacologic, metabolic, and physical stimuli were studied. a modified bovine serum albumin density gradient purification method consistently provided mast cell preparations which were 95% or more pure, without apparent damage, and a 73% recovery of the mast cells applied to the gradients. the measured camp in unstimulated mast cells was high, a mean of 16 picomol ... | 1975 | 47363 |
| a hemolytic plaque assay for the detection of direct and indirect antibody-forming cells to keyhole limpet hemocyanin. | a procedure is described for the in vitro enumeration of individual lymphoid cells producing antibody against keyhole limpet hemocyanin (klh) by a modification of the hemolytic plaque technique. optimal conditions for the coupling of klh to sheep erythrocytes by chromic chloride are described. plaque-forming cells are detected in a liquid monolayer slide chamber assay system. the kinetics of the in vivo primary and secondary pfc responses of both rabbit popliteal lymph node cells and mouse splee ... | 1975 | 47369 |
| a comparison of the farr technique with equilibrium dialysis for measurement of antibody concentration and affinity. | the farr technique has been compared with equilibrium dialysis in regard to its ability to measure antibody concentration and affinity. an excellent qualitative agreement between the two techniques was obtained. quantitatively, the farr technique tended to underestimate antibody concentration and overestimate antibody affinity. it is likely that these observations are due to the failure of some low affinity antibodies to bind hapten under the conditions of the farr assay. | 1975 | 47370 |
| rapid and high production of precipitating antibodies in rats. | | 1975 | 47372 |
| cardiac histamine-ouabain interaction: potentiation by ouabain of the arrhythmogenic effects of histamine. | cardiac effects of histamine include stimulation of sinus rate and ventricular contractile force, impairment of atrioventricular conduction and increase in ventricular automaticity. atrioventricular block and increase in ventricular automaticity are common features of digitalis toxicity. the purpose of the present investigation was to study the influence of low concentrations of ouabain on the cardiac effects of immunologically released and administered histamine. hearts excised from guinea pigs ... | 1975 | 47388 |
| studies on the pathogenesis of rheumatic heart disease. an immunological relationship between the polysaccharide of group a streptococcus and the glycoprotein of heart valve. | the immunization of rabbits with bovine heart valve homogenates induced the formation of antibody which reacted with ctc soluble extracts and also proteolytic enzyme treated fractions. analysis by fractionation of materials revealed 2 peaks and both peaks contain monosaccharides. the first peak was more antigenic to anti-valvular sera. the cross-reactivity between the valvular glycoprotein and group a streptococcal polysaccharides was confirmed by the immunological inhibition tests. widespread d ... | 1975 | 47405 |
| the antigenic determinant of the lh-releasing hormone for three different antisera. | antigenic determinants of lh-releasing hormone (lh-rh) were investigated by testing the cross-reaction of lh-rh analogues and fragments in lh-rh radioimmunoassay (ria) systems using 3 different antisera against the lh-rh decapeptide. rabbit antiserum no. 419 was generated against lh-rh adsorbed on polyvinylpyrrolidone (pvp). antisera nos. 710 and 742 were produced by immunizing rabbits with lh-rh conjugated either with bovine serum albumin through its c-terminus, or with human serum albumin thro ... | 1975 | 47682 |
| quantification of coomassie blue stained proteins in polyacrylamide gels based on analyses of eluted dye. | | 1975 | 47719 |
| effect of humoral antibodies on the course of theileria parva infection (east coast fever) of cattle. | a study was made of the effects of serum and gamma-globulins from vaccinated cattle (donor cattle) given to cattle (principals) exposed to theileria parva infection (east coast fever (ecf)). the principals were treated with normal serum, with serum containing high antibody titer against t parva (as evaluated by the indirect fluorescent antibody (ifa) technique), or with globulins prepared from normal and from immune serums. for exposure, the principals were inoculated with standardized suspensio ... | 1975 | 47727 |
| comparison of the specificty of human and bovine tuberculin ppd for testing cattle. 1--republic of ireland. | a tuberculin testing trial in cattle was carried out in the republic of ireland to compare the specificity for bovine tuberculosis of a human purified protein derivative (ppd) tuberculin (weybridge) with that of a bovine ppd (rotterdam), and to determine whether discrimination between specific and non-specific reactions to mammalian tuberculin is better with doses of tuberculins smaller than those traditonally used for testing cattle. tests were carried out in 510 cattle, 395 of which were shown ... | 1975 | 47750 |
| comparison of the specificity of human and bovine tuberculin ppd for testing cattle. 2. south-eastern england. | a tuberculin testing trial was carried out in eight counties of south-eastern england to compare the specificity for bovine tuberculosis of weybridge human ppd with that of rotterdam bovine ppd. the matching of these two tuberculins for potency in naturally infected cattle had already been established, the bovine ppd being approximately one-and-a-half times more potent than the human ppd per unit of weight. in 1110 cattle in 25 herds with histories of long-standing freedom from tuberculosis and ... | 1975 | 47751 |
| comparison of the specificity of human and bovine tuberculin ppf for testing cattle. 3. national trial in great britain. | a field trial on a country-wide basis was undertaken to compare the specificity for bovine tuberculosis of single and comparative tuberculin tests in cattle using either weybridge human or weybridge bovine ppd. the tests were made on 10,305 cattle in 179 herds distributed throughout all regions of england, scotland and wales. results showed that a comparative tuberculin test using avian ppd with either human or bovine ppd had a much higher efficiency than a single injection of mammalian tubercul ... | 1975 | 47752 |
| large peptides of bovine and guinea pig myelin basic proteins produced by limited peptic hydrolysis. | bovine and guinea pig myelin basic proteins were cleaved with pepsin at ph 3.0 or ph 6.0 (enzyme/substrate, 1:500, w/w), and the peptides were isolated and identified. at ph 3.0 cleavage of the bovine protein occurred principally at three sites: phe-phe (88-89), phe-phe (42-43), and leu-asp (36-37). minor cleavages occurred at leu-ser (110-111), phe-ser (113-114), and ile-phe (152-153). a study of the time course of the hydrolysis showed that the reaction was biphasic; nearly all of the protein ... | 1975 | 47756 |
| exposure of histone antigenic determinants in chromatin. | the exposure of antigenic determinants of histones present in "native" chromatin was studied by: (1) testing their ability to elicit anti-histone antibodies and (2) measuring their ability to interact with anti-histone sera. to this end, antisera specific to purified histone fractions and to purified rat liver chromatin were elicited in rabbits. the anti-chromatin sera did not react with pure histone fractions and pure histone fractions f2b, f3, f2a1, and f2a2 failed to inhibit the complement fi ... | 1975 | 47758 |
| phosphoprotein phosphatases for myelin basic protein in myelin and cytosol fractions of brain. | phosphoprotein phosphatase (phosphoprotein phosphohydrolase ec 3.1.3.16) activity for myelin basic protein was found to be present in the myelin fraction of rat brain. the enzyme activity was in a latent form and solubilized by 0.2% triton x-100 treatment with about 50% increase of activity. the cytosol fraction from bovine brain also had phosphoprotein phosphatase activity for myelin basic protein, which was resolved into at least two peaks of activity on deae-cellulose column chromatography. m ... | 1975 | 47761 |
| the phosphorylation site associated with the oxidation of exogenous donors of electrons to photosystem i. | 1. the photosystem i-mediated transfer of electrons from diaminodurene, diaminotolune and reduced 2,6-dichlorophenolindophenol to methylviologen is optimal at ph 8-8.5, where phosphorylation is also maximal. in the presence of superoxide dismutase, the efficiency of phosphorylation rises from smaller than or equal to 0.1 at ph 6.5 to 0.6-0.7 at ph 8-8.5, regardless of the exogenous electron donor used. 2. the apparent km (at ph 8.1) for diaminodurene is 6-10-minus 4 m and for diaminotoluene is 1 ... | 1975 | 47763 |
| immunological activities of rat lymphocytes. ii. isolation of suppressor cells of the mitogenic response of rat thoracic duct lymphocytes. | | 1975 | 47788 |
| myxoviruses. | myxoviruses can be divided into 2 groups, orthomyxoviruses and paramyxoviruses. the former comprise the influenza group which is subdivided into types a,b and c. influenza b and c are purely human pathogens but influenza a, which includes a large number of antigenic subtypes, occurs in nature in pigs, horses, birds and man. all influenza a viruses irrespective of origin are chemically, biologically and genetically related. the epidemics which they cause are curious and puzzling and are an import ... | 1975 | 47825 |
| studies on parainfluenza-3 virus neuraminidase. | various parainfluenza-3 virus (piv-3) strains differ in neuraminidase (na) activity, some being neuraminidase strong (nas) and some neuraminidase weak (naw). no difference in the ability of these strains to elicit antibody activity to hemagglutinin (ha) or na of piv-3 was observed. samples of formalin-treated piv-3 released more n-acteylneuraminic acid (nana) than samples of piv-3 not treated with formalin when these samples were incubated with the substrate sialolactose for 16 h at 37 degrees c ... | 1975 | 47826 |
| demonstration of a unique antigenic specificity for the collagen alpha1 (ii) chain from cartilaginous tissue. | the rabbit antibody response to native collagen (chain composition [alpha1(ii)3) from cartilaginous tissue, has been examined by agglutination assays, gel diffusion, haemagglutination-inhibition studies, and immunoadsorption. the results show that the rabbit antibody response to the cartilage-type collagen is characterized by considerable reactivity to both helical [alpha1(ii)]3 as well as alpha1(ii) chains. this is in contrast to the rat antibody response to the same antigens where titres are g ... | 1975 | 47839 |
| letters to the editor: phosphotungstate:phospholipid interactions: direct staining and plasmal reactions. | | 1975 | 47873 |
| the role of t cells in igg production; thymus-dependent antigens induce b cell memory in the absence of t cells. | b cell memory was shown to develop in congenitally athymic (nu/nu) mice after injection with small amounts of thymus-dependent antigens, in particular heterologous serum proteins, such as fown gamma-globulin (fgg) or dnp-bovine-serum albumin (dnp-bsa). large doses of proteins (10 mg) tended to produce a specific b cell unresponsiveness, although there was still some evidence of b cell priming. the antigen did not have to be in a multivalent form to interact with b cell so as to induce immunologi ... | 1975 | 47875 |
| preventive effect of hapten-reactive thymus-derived helper lymphocytes on the tolerance induction in hapten-specific precursors of antibody-forming cells. | the role(s) of helper t lymphocytes in preventing or altering tolerance induction in dnp-specific b lymphocytes was studied. as dnp-reactive helper t cells were reactive against the dnp-portion of the dnp-d-gl molecule, we could probe definitively the physiological role of helper t cells in preventing tolerance induction in b lymphocytes by dnp-d-gl. the results demonstrated that the induction of dnp-specific b cell tolerance by dnp-d-gl can be completely prevented by the presence of dnp-reactiv ... | 1975 | 47879 |
| isolation and analysis of the mechanism of action of an inactivator of c4b in normal human serum. | a complement regulatory principle, c4b inactivator, was isolated in a partially purified form from normal human serum. the c4b inactivator, a beta1-globulin with an approximate mol wt of 88,000 daltons, and which may be identical to c3b inactivator, cleaved c4b in free solution or on the surface of cells and rendered it unable to participate in hemolytic reactions or to interact with cells, having receptors for c4b. c/b inactivator functioned by cleaving the alpha-polypeptide chain of c4b at a s ... | 1975 | 47888 |
| evidence for the clonal abortion theory of b-lymphocyte tolerance. | this paper deals with the behavior of adult mouse bone marrow cells placed in tissue culture with or without antigen, and subsequently assessed for immune competence after adoptive transfer into lethally x-irradiated, syngeneic hosts. attention was focussed on b lymphocytes through using hapten human gamma globulin (hgg) preparations as putative tolerogens in tissue culture, the t-cell-independent antigens dnp-pol and nip-pol as challenge injections in adoptive hosts, and numbers of hapten-speci ... | 1975 | 47889 |
| separation of antigen-specific lymphocytes. i. enrichment of antigen-binding cells. | normal mouse spleen cells were fractionated in dishes coated with thin layers of dnp-gelatin or nip-gelatin, which were insoluble at 4 degrees c. highly viable cells were recovered from the dishes by melting the gel at 37 degrees c. nip3- gelatin layers bound approximately 0.1% and dnp4-gelatin layers 0.5% of normal spleen cells. increasing numbers of low affinity cells were bound with increasing dnp density of the adsorbent. the binding to insoluble dnp-gelatin was hapten-specific since it was ... | 1975 | 47891 |
| the in vitro induction of immunological tolerance in the b lymphocyte by oligovalent thymus-dependent antigens. | b-cell tolerance has been induced by oligovalent thymus-dependent antigens in an entirely in vitro system. dissociated spleen cells from congenitally athymic (nu/nu) mice were preincubated for 24 h with 0.1 -- 1 mg/ml of either fowl gamma globulin (fgg) of dnp-human gamma globulin (dnp-hgg). after washing, the cells were tested for the ability to mount in vitro, thymus-independent responses against fgg and dnp. a state of specific responsiveness to either fgg or dnp was thus demonstrated. featur ... | 1975 | 47897 |
| spasmolytic constituents of cedrus deodara (roxb.) loud: pharmacological evaluation of himachalol. | himachalol has been identified as the major antispasmodic constituent in the wood of cedrus deodara. the pharmacological studies of himachalol on various isolated smooth muscles (guinea pig ileum, rabbit jejunum, rat uterus, and guinea pig seminal vesicle) and against different agonists (acetylcholine, histamine, serotonin, nicotine, and barium chloride) indicated spasmolytic activity similar to that of papaverine. it was a more potent antagonist of barium chloride-induced spasm of guinea pig il ... | 1975 | 47907 |
| letter: rotaviruses of man and animals. | | 1975 | 47983 |
| detection of circulating immune complexes in pathological human sera. | the results of attempts to detect circulating immune complexes in sera by two methods are described. both rely on the binding of the first complement component (c1) to the complex for its detection. the results, using these methods, of attempts to show which patients have complexes which contain nucleic acid and hence possibly viral antigens in the complexes are discussed. the anti-complementary complexes found in some patients seem to be associated with immune-complex vascular disease. | 1975 | 47998 |
| development and application of sequence-specific radioimmunoassays for analysis of the metabolism of parathyroid hormone. | | 1975 | 48177 |
| cell-surface-associated nucleic acid in tumorigenic cells made visible with platinum-pyrimidine complexes by electron microscopy. | platinum-pyrimidine complexes have been used as the sole electron-dense stains for electron microscopy on 18 types of mammalian cells. these recently discovered complexes react avidly with nucleic acids in vitro, are antitumor agents, and are highly soluble in water. in cells, they are selective for regions rich in nucleic acid and make visible the cellular chromatin, nucleolus, and ribosomes. in addition, cells that are tumorigenic exhibit electron-dense patches at the plasma membrane; normal c ... | 1975 | 48252 |
| replication of ipn virus: a cytochemical and biochemical study in swt cells. | although ipn virus failed to multiply at 30 degrees, it replicated at 16 degrees and 22 degrees in swt cells. at 22 degrees the viral eclipse period lasted nearly 6 hr with maximal virion titers attained by 24 hr, whereas replication at 16 degrees was much slower. the replication of the virion was inhibited by 0.05 mug/ml of ad which did not interfere with the production of reovirus. biochemical studies revealed that cellular dna synthesis was markedly reduced (greater than 50%) soon after infec ... | 1975 | 48260 |
| steric hindrance of con a receptor sites by antigen: a possible explanation of ir regulated responses. | spleen cells from mice that respond poorly (c57bl/6) or well (cba, c3h/hej and b6d2f1) to dnp-bgg, an antigen under ir gene regulation, were cultured with the t cell mitogen con a and varying concentrations of dnp-bgg and dnp-klh. it was found that dnp-bgg dpressed the responses of c57bl/6 spleen cells to con a stimulation to a much greater degree than did dnp-klh; the con a stimulated responses of spleen cells from the other strains were impaired equally and less severely by both antigens. the ... | 1975 | 48261 |
| heterophile antigens and antibodies in transplantation and tumors. | | 1975 | 48298 |
| [synchronization of estrus in heifers by means of gestagenic preparations]. | studied were the synchronizing effect, the course of treatment, the combination of the gestagens cap and mga with pms as well as their effect on the conception rate in heifers. a total of 143 heifers of the bulgarian brown breed (89 test and 59 control), aged 17--18 months, weighing 350--360 kg on an average, were used in the experiment, divided into three test and three control groups. i test group. twenty-five animals were given daily one tablet of synchrorosyn-peach, the tablet containing 10 ... | 1975 | 48304 |